30 results about "Nitidine chloride" patented technology

The invention discloses a method for preparing target antineoplastic drug chlorinated nitidine compound, products thereof and an injection containing the products. The method for preparing the target antineoplastic drug chlorinated nitidine compound comprises the steps of pre-treating the chlorinated nitidine to acquire carboxylic acid-modified chlorinated nitidine, then using amphiphilic polymers to implement non-covalent functionalized modification on the outer wall of a single-wall carbon nano tube that is cut to be 20-500 nm long, and coupling the carboxylic acid-modified chlorinated nitidine with the single-wall carbon nano tube after the non-covalent functionalized modification by means of esterification and amidation reactions to acquire the compound. The preparation method of the compound is simple, the solubility of the resulting products can reach 5.0mg / mL, and the antineoplastic target is strong, thus favorably settling the problems of low solubility and strong toxicity of the chlorinated nitidine; in addition the invention can be applied clinically as a substitute for the chlorinated nitidine and provides a new water-soluble compound for the clinical trial of the chlorinated nitidine.

The invention discloses an application of nitidine chloride in resisting osteoporosis and in preventing and treating bone loss diseases caused by osteoclasts; the inventor, through researches, discovers that the nitidine chloride can inhibit the formation of the osteoclasts and a bone absorption function in a dose-dependent mode for the first time; it discovers that the nitidine chloride, by intervening RANKL-activated NF-kB and NFAT signaling pathways, can develop an effect of inhibiting the osteoclasts; and by establishing an ovariectomized osteoporosis mouse model, it further provides the effect of the nitidine chloride on the osteoclasts. The nitidine chloride can be used as an ideal medicine for preventing and treating the bone loss diseases caused by the osteoclasts in the future.

The invention relates to a traditional Chinese medicine extract having poultry and livestock mycoplasma resistant activity. The traditional Chinese medicine extract contains any one or more of the following components: 1-512 mu g / mL of nitidine chloride, 1-512 mu g / mL of ethoxychelerythrine, 1-512 mu g / mL of chelerythrine, 1-512 mu g / mL of sanguinarine, 1-512 mu g / mL of coptisine, 1-512 mu g / mL of palmatine, 1-512 mu g / mL of epiberberine, and 1-512 mu g / mL of glycyrrhetinic acid. The invention further provides a traditional Chinese medicine composition having poultry and livestock mycoplasma resistant activity. Both the traditional Chinese medicine extract and the traditional Chinese medicine composition provided by the invention have the stronger effect for inhibiting poultry and livestock mycoplasma activity in vitro, are safe, green and free from medicine residue, and can be applied to treating and preventing poultry and livestock mycoplasma diseases.

The invention discloses a double ternary two-dimensional column high-performance liquid chromatography method for determining the content of acanthine chloride in toothpaste, and belongs to the technical field of high-performance liquid chromatography for determining substance content. Including the following steps: (1) drawing a standard curve; (2) placing the toothpaste to be tested in a centrifuge tube with a stopper and distilling it to volume with methanol; (3) preparing the solution to be tested; (4) using double ternary high performance liquid chromatography Two-dimensional column separation method, ultraviolet detector to determine the chlorinated chlorinated chlorinated, and the content of chlorinated chlorinated was obtained according to the standard curve. The method of the invention is simple and fast in operation, good in selectivity and good in reproducibility; it can make the chromatographic peak of the target analyte and the impurity chromatographic peak achieve better baseline separation, can eliminate the interference of interfering substances in the toothpaste matrix, and accurately detect the toothpaste Quantitative analysis of dihedral chloride in the sample, and the separation effect is good and the linear range is wide.

The invention discloses a method for purifying nitidine chloride. The method comprises the following steps: after smashing a nitidine medicinal material, reflux-extracting by using acidity alcohol solution, merging acid alcohol extracting solution, regulating the pH value by using acid-base, stewing and filtering; alcohol-precipitating, refrigerating, filtering, and concentrating to obtain the extracting solution as sample solution; uniformly mixing the sample solution and normal phase silica gel or aluminium oxide, drying by distillation, separating and purifying through the normal phase silica gel or aluminium oxide, eluting by using ethyl acetate-absolute ethyl alcohol, recycling a solvent, collecting fractions, dissolving by using methyl alcohol, merging the fractions with the high content through the TLC identification and recrystallizing, to obtain a product as the nitidine chloride; and executing the HPLC assay, wherein the transfer rate is more than 80%, and the crystallization purity is higher than 90%. The method is capable of efficiently separating and purifying the nitidine chloride, and is suitable for the large-scale production. The toxicity is small, few solvents are used, the economic value is high, the environmental pollution is small, and the purity is high.