Thin-layer chromatographic identification method for Zanthoxylum dissitum Hemsl

A thin-layer chromatography and single-sided acupuncture technology, which is applied in the field of medicine, can solve the problems of mixed use of single-sided acupuncture and double-sided acupuncture, lack of quality control standards for single-sided acupuncture, etc., and achieves the effects of low cost, high accuracy and clear spots.

Pending Publication Date: 2021-05-04
ZHUZHOU QIANJIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of perfect quality control standards for single-sided needles, the confusion and use of single-sided needles and double-sided needles is serious.

Method used

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  • Thin-layer chromatographic identification method for Zanthoxylum dissitum Hemsl
  • Thin-layer chromatographic identification method for Zanthoxylum dissitum Hemsl
  • Thin-layer chromatographic identification method for Zanthoxylum dissitum Hemsl

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079]Example 1 Single-sided needle layer chromatographic identification method

[0080]Take a single-sided needle for the test, two-sided needle for the test, the single-sided needle product is sample, while the white ethanol solvent is controlled, and thin layer chromatographic analysis, including the following steps:

[0081](1) Preparation of the solution solution:

[0082]Take 2G Test Powder (No. 2 Sieve), set the tapered bottle, 40 ml of ethanol, sealed, ultrasonic treatment for 60 min, filtration, water bath, residue, and 2 ml of ethanol dissolve, as a test solution.

[0083](2) Preparation of the solution solution:

[0084]Take a monolithic alkali control, add 1 mg / ml of a solution solution.

[0085]Take two-squeezepine control, add ethanol to the solution of 0.5 mg / ml of the reaction solution;

[0086]The ethanol used is 95% ethanol.

[0087](3) Thin layer chromatographic detection: (refer to China Pharmacopoeia 2015 Edition Appendix 0502)

[0088]Take 5 μl of the test solution, 8 μl of the test ...

Embodiment 2

[0090]Example 2 Single-sided needle layer chromatographic identification method

[0091]A single-sided thin layer chromatographic identification method, including the steps of:

[0092](1) Preparation of the solution solution:

[0093]Take 1.2G single-sided needle for the test powder (2 sieve), tapered bottle, 30 ml of ethanol, sealed, ultrasonic treatment 90min, filtration, water bath, residue plus 2mL ethanol dissolved, as a test product Solution;

[0094](2) Preparation of the solution solution:

[0095]Take a magnolkine control, add ethanol to the solution of 0.8 mg / ml of a control solution;

[0096]Take two-squeezepine control, add ethanol to the solution of 0.4 mg / ml of the reaction solution;

[0097](3) Thin layer chromatographic detection: (refer to China Pharmacopoeia 2015 Edition Appendix 0502)

[0098]10 μL of the test solution, 10 μl of the test solution, separately on the same silicone GF254 sheet, the dot pattern is 8 mm strips, with trichloromethane: methanol: water: diethylamine = 35: 8...

Embodiment 3

[0100]Example 3 Single-sided needle layer chromatographic identification method

[0101]A single-sided thin layer chromatographic identification method, including the steps of:

[0102](1) Preparation of the solution solution:

[0103]Take 2.8g single-sided needle for the test powder (two sieves), set the tapered bottle, 50 ml of ethanol, sealed, ultrasonic treatment for 60 min, filter, water bath, residue plus 2ml ethanol dissolve, as a test product Solution;

[0104](2) Preparation of the solution solution:

[0105]Take a magnoli-carnitine control, add ethanol to the solution of 1.5 mg / mL of the solution;

[0106]Take the chlorinated oxide, add 0.7 mg / ml of the solution solution to the ethanol;

[0107](3) Thin layer chromatographic detection: (refer to China Pharmacopoeia 2015 Edition Appendix 0502)

[0108]8 μL of the test solution, 5 μL of the test solution, separately on the same silicone GF254 thin layer plate, the dot pattern is 8 mm strips, with trichloromethane: methanol: water: diethylamine ...

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Abstract

The invention discloses a thin-layer chromatographic identification method for Zanthoxylum dissitum Hemsl. According to the thin-layer chromatographic identification method, magnoflorine and nitidine chloride are taken as reference substances, specific spots of thin-layer chromatography of the Zanthoxylum dissitum Hemsl are determined, and the method can be used for identifying the Zanthoxylum dissitum Hemsl and distinguishing the Zanthoxylum dissitum Hemsl and the zanthoxylum nitidum. The method is good in repeatability, high in accuracy degree, good in separation, clear in spots, simple, feasible, rapid, low in cost and suitable for distinguishing Zanthoxylum dissitum Hemsl and Zanthoxylum nitidum and quality control and authenticity identification of Zanthoxylum dissitum medicinal materials. The method is good in safety, economical, environment-friendly, safe, high in efficiency and good in application prospect.

Description

Technical field[0001]The invention belongs to the field of medical technology. More specifically, it is related to a single-sided thin layer chromatographic identification method.Background technique[0002]Single-sided needle is a zanthoxylun, Zanthoxylun, Zanthoxylumdissitum Hemsley, and Z.echinocarpum Hemsley, often greenery, mainly in the name of "National Chinese Herbal Compilation". It is included in the Chinese medicine monograph contained in "China Plant", "Hunan Chinese Medicine Standard", "Choice of China Herbal Resources" in Guangxi. Folk is often used to treat low back pain, toothache, bruises, hernia pain, and women's moonlight. Roots, stems, leafs can be used in medicine, high medicinal value, attending toothache, low back pain, women have many menstruation, postpartum menstrual irregularities. Single-sided needles are mainly used as the main raw materials for gynecologic capsules, gynecologic films, etc., and is widely used.[0003]The two-side needle as a long history of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/94G01N30/95
CPCG01N30/90G01N30/94G01N30/95
Inventor 凌勇根龚云白璐王月秀何娟娟
Owner ZHUZHOU QIANJIN PHARMA
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