11 results about "Magnoflorine" patented technology

The invention discloses a method for determining four mangnolia officinalis extracts in import and export cosmetics, which is used for detecting magnolol, honokiol, magnoflorine and magnoliavine, and comprises the following steps: S1, uniformly mixing a cosmetic sample with a solvent; s2, after uniform mixing, carrying out vortex oscillation treatment on the sample; s3, after vortex oscillation treatment, carrying out ultrasonic extraction on the sample at room temperature to obtain an extracting solution; s4, mixing the extracting solution with an adsorbent to obtain a purified to-be-detected solution; and S5, filtering the to-be-detected liquid, and determining the to-be-detected liquid through LC-MS / MS to obtain a detection result. According to the present invention, the target object in the cosmetics with different matrix types can be effectively represented, the core functional components such as magnolol and honokiol in the cortex magnoliae officinalis extract can be detected, the qualitative and quantitative analysis can be further performed on the potential supervision risk substance, and the dual functions of quality evaluation and safety supervision can be provided for the cosmetics.

The present invention relates to the technical field of drug analysis, and specifically relates to an analytical method for Litsea lancilimba formula granules. The Litsea lancilimba formula granules are formed by decocting Litsea lancilimba decoction pieces in water while collecting volatile oil and carrying out concentration, drying, and granulation. The analytical method comprises the following items: descriptions, identification, tests, extractives, characteristic chromatographic fingerprints, and assay. The identification method uses thin-layer chromatography for identification; in the characteristic chromatographic fingerprints, by taking the peak corresponding to the reference peak of magnoflorine reference substance as an S peak, high performance liquid chromatography is used to mark four characteristic peaks; and the assay is to determine the content of magnoflorine. The analytical method established in the present invention improves the analytical requirements for medicinal materials, decoction pieces, standard decoctions and formula granules of Litsea lancilimba, which helps to establish a perfect quality control system for various products of Litsea lancilimba, and helps to ensure the quality and clinical efficacy of the Litsea lancilimba formula granules.

This invention provides a method for constructing HPLC characteristic chromatograms of processed medicinal slices, standard decoctions, and formulated granules of *Sophora flavescens*, comprising: A) dissolving and extracting the test sample raw material using a solvent to obtain a test solution; B) determining the test solution using high-performance liquid chromatography (HPLC) to obtain HPLC characteristic chromatograms of processed medicinal slices, standard decoctions, and formulated granules of *Sophora flavescens*; the HPLC chromatographic conditions are as follows: a C18 column; mobile phase A is acetonitrile solution, mobile phase B is 0.05% trifluoroacetic acid aqueous solution, and gradient elution is performed; specifically, the gradient elution is as follows: 0–15 min, phase A: 5%–11%, phase B: 95%–89%; 15–40 min, phase A: 11%–18%, phase B: 89%–82%; 40–60 min, phase A: 18%–21%, phase B: 82%–79%. This invention employs high-performance liquid chromatography (HPLC) with acetonitrile-0.05% trifluoroacetic acid solution as the mobile phase for gradient elution. Using magnoflorine, isocoryne, styraxine, and styraxine as references, a method for establishing characteristic HPLC chromatograms of processed slices, standard decoctions, and formulated granules of *Sophora flavescens* was developed. This provides more scientific technical means for controlling the medicinal quality of processed slices, standard decoctions, and formulated granules of *Sophora flavescens*.

The invention discloses a method for simultaneously determining seven mass markers (Q-Marker) in serum of traditional Chinese medicine particles for treating cervical pain, which is characterized in that the seven mass markers are magnoflorine, puerarin, tetrahydropalmatine, paeoniflorin, notoginsenoside R1, ligustilide and decuricin; the method comprises the following steps: adding a serum sample into acetonitrile and acetone, carrying out vortex and centrifugation, taking a supernatant, loading the supernatant into a Hybrid SPEcolumn, washing with water to remove salt and polar interferents, eluting a target analyte with acetonitrile, filtering the eluent through a microfiltration membrane, carrying out sample introduction determination, and carrying out quantitative analysis by adopting a high performance liquid chromatography-mass spectrometry method and an electrospray ion source. And quantitative analysis is carried out in a positive / negative ion multi-reaction monitoring detection mode. The method is efficient, sensitive and high in selectivity, and solves the problems of serious matrix interference, indefinite target components, difficulty in simultaneous analysis of multiple components and the like in the prior art.

The invention discloses a method for producing aporphine alkaloid and recombinant bacteria used by the method. According to the invention, the synthetic route of the aporphine alkaloid is divided into four modules for research, and the de novo synthesis of the aporphine alkaloid is realized through various strategies; wherein the original strain is an IMX581 yeast strain. Experiments prove that the engineering strain constructed by the invention can be used for efficiently producing aporphine alkaloids such as (R)-croflorin, (R)-glaziovine, (S)-glaziovine, magnoflorin and the like. The aporphine alkaloids disclosed by the invention can be used for efficiently producing aporphine alkaloids such as (R)-croflorin, (R)-glaziovine, (S)-glaziovine, magnoflorin and the like. The method has an important application value.

The extraction method of effective components of traditional Chinese medicine composition for treating knee osteoarthritis based on entropy weight method and response surface method belongs to the technical field of traditional Chinese medicine extraction, establishes the content determination method of index components in the composition, i.e., sinomenine, magnoflorine, paeonol glycoside, paeoniflorin and dioscin, and carries out methodology investigation; the comprehensive score of index component content is calculated by the entropy weight method as an evaluation index, the influence factors are ethanol concentration, solid-liquid ratio and soaking time, the experiment is designed by applying the response surface method, the index weight is determined by the comprehensive weighting theory of evaluation index, the comprehensive score is calculated, the multiple quadratic regression model of comprehensive score and investigation factors is established, the variance analysis is carried out, the optimal process is predicted and verified, and the optimized extraction process parameters of Jiantongkang are determined. The extraction condition optimization research of index components of the composition based on the entropy weight method and the response surface method is strong in pertinence, simple in operation, high in accuracy, scientific and reasonable in process, and is suitable for the content determination of medicinal material effective components.

This invention relates to a method for constructing the characteristic chromatogram of the traditional Chinese medicine Zhikang Capsules, as well as the chromatogram and its application. The method involves gradient elution using high-performance liquid chromatography (HPLC) to determine the contents of catechins, magnoflorine, berberine hydrochloride, and 1,4-bis[4-(glucoseoxy)benzyl]-2-isobutyl malate, thus constructing the characteristic HPLC chromatogram of Zhikang Capsules. Nine characteristic peaks were identified as the characteristic chromatogram, covering major components such as alkaloids, phenols, anthraquinones, and glycosides. A quality evaluation system for Zhikang Capsules was constructed. This method exhibits good specificity, precision, repeatability, and solution stability, and can be used to standardize the production of Zhikang Capsules and provide comprehensive quality control to ensure the uniformity and stability of the product's intrinsic quality.

The invention belongs to the technical field of medicines, and finds that aporphine alkaloids, especially magnoline and nuciferine, can reduce the BUN level and SCr level of a cisplatin-induced acute kidney injury model mouse through a large number of experimental screening, can obviously relieve cisplatin-induced kidney function impairment, and can be used for preparing medicines for treating acute kidney injury. The compound can be used as a medicine for reducing adverse reaction of renal function impairment caused by cis-platinum.

The application belongs to the technical field of traditional Chinese medicine detection, and specifically provides a construction method and a quality detection method for characteristic chromatograms of Sarcandra glabra medicinal preparation, wherein octadecylsilane bonded silica gel is used as a filler, acetonitrile is used as mobile phase A, and a water solution containing potassium dihydrogen phosphate is used as mobile phase B, a gradient elution program is continuously optimized, the column temperature is controlled to be 23-27 DEG C, 11 common characteristic peaks are finally obtained, 9 common characteristic peaks are effectively separated, the characteristic chromatogram obtained has a smooth baseline, the characteristic peaks have good peak shapes, and the detection time is short, thereby providing a scientific basis for comprehensively establishing the quality control standard of Sarcandra glabra formula granules. Moreover, the peak positions of tryptophan, syringin, isovanillin and magnoflorine can be accurately positioned, the known information of the characteristic peaks is increased, and the integrity and characteristics of Sarcandra glabra medicinal preparation (for example, formula granules and other preparations) are fully reflected.

The application belongs to the technical field of natural medicine chemistry, and particularly relates to a method for extracting compounds in Magnolia officinalis by using a low eutectic solvent and a detection method. Magnolia officinalis powder and a low eutectic solvent are mixed, and then soaking and ultrasonic extraction are sequentially performed to obtain an extraction liquid containing target compounds; the target compounds are magnoliabine, syringin, magnolol and honokiol. The low eutectic solvent is used to assist ultrasonic extraction of the Magnolia officinalis powder, and strong hydrogen bond interaction is formed between the low eutectic solvent and the target compounds, which is beneficial to the extraction of the target compounds, so that the extraction rate of the four target compounds in the Magnolia officinalis powder is improved.

The present application belongs to the technical field of medicine detection, and particularly relates to a detection method of large-fruit Litsea cubeba formula granules. The large-fruit Litsea cubeba formula granules are prepared by decocting large-fruit Litsea cubeba decoction pieces with water, collecting volatile oil, concentrating, drying and granulating. The detection method comprises the items of appearance, identification, examination, extract, characteristic spectrum and content determination. The identification method is identification by thin layer chromatography. The characteristic spectrum is obtained by high performance liquid chromatography, and four characteristic peaks are indicated with the peak corresponding to the reference peak of magnoflorine as S peak. The content determination is detection of the content of magnoflorine. The detection method established by the present application improves the detection requirements of large-fruit Litsea cubeba medicinal materials, decoction pieces, standard decoction and formula granules, and is helpful to establish a perfect quality control system of large-fruit Litsea cubeba products, and to guarantee the quality and clinical efficacy of large-fruit Litsea cubeba formula granules.