21 results about "Magnoflorine" patented technology

The invention belongs to the technical field of biological medicine, and particularly relates to application of magnoflorine in preparation of a product for preventing / treating liver injury. The invention discusses the protective effect and mechanism of magnoflorine on MTX hepatotoxicity for the first time, and magnoflorine regulates ROS accumulation and mPTP permeability through targeting GSK3beta to inhibit pyroptosis of hepatocytes so as to reduce MTX hepatotoxicity. A new target and a new choice are provided for the prevention and treatment of MTX hepatotoxicity, and a research foundation is laid for the research of a new magnoflorine component liver-protecting drug.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly provides a construction method and a quality detection method of a specific chromatogram of a tinospora sinensis merr medicinal preparation, octadecylsilane chemically bonded silica is used as a filler, acetonitrile is used as a mobile phase A, an aqueous solution containing monopotassium phosphate is used as a mobile phase B, and a gradient elution procedure is continuously optimized; according to the method, the column temperature is controlled to be 23-27 DEG C, 11 common characteristic peaks are finally obtained, effective separation of 9 common characteristic peaks is achieved, the obtained characteristic chromatogram is stable in base line, good in characteristic peak shape and short in detection time, and a scientific basis is provided for comprehensively establishing the quality control standard of the tinospora sinensis merr formula granules. And moreover, the peak positions of tryptophan, syringin, isovanillin and magnoflorine can be accurately positioned, the known information of characteristic peaks is increased, and the integrity and characteristics of the tinospora sinensis merr medicinal preparations (such as preparations such as formula granules) are fully reflected.

The invention discloses a content determination method of a traditional Chinese medicine Qianjin coptis chinensis pill, which comprises the following steps: a test solution and a reference solution of the Qianjin coptis chinensis pill are taken for detection under the chromatographic conditions that a chromatographic column with octadecylsilane chemically bonded silica as a filler is adopted, a mobile phase A is selected from acetonitrile, and a mobile phase B is a buffer saline solution; and obtaining content information of the Qianjin coptis pill according to a detection result. The reference substances are catalpol, berberine hydrochloride, palmatine hydrochloride, epiberberine, coptisine, caffeic acid, ferulic acid, magnoflorine, jateorhizine and columbamine. The chemical components of the Qianjin coptis pill are comprehensively and systematically analyzed, and a technical method is provided for perfecting and improving the medicine quality standard of the Qianjin coptis pill and better controlling the medicine quality.

The invention discloses a method for determining four mangnolia officinalis extracts in import and export cosmetics, which is used for detecting magnolol, honokiol, magnoflorine and magnoliavine, and comprises the following steps: S1, uniformly mixing a cosmetic sample with a solvent; s2, after uniform mixing, carrying out vortex oscillation treatment on the sample; s3, after vortex oscillation treatment, carrying out ultrasonic extraction on the sample at room temperature to obtain an extracting solution; s4, mixing the extracting solution with an adsorbent to obtain a purified to-be-detected solution; and S5, filtering the to-be-detected liquid, and determining the to-be-detected liquid through LC-MS / MS to obtain a detection result. According to the present invention, the target object in the cosmetics with different matrix types can be effectively represented, the core functional components such as magnolol and honokiol in the cortex magnoliae officinalis extract can be detected, the qualitative and quantitative analysis can be further performed on the potential supervision risk substance, and the dual functions of quality evaluation and safety supervision can be provided for the cosmetics.

The present invention provides a method for establishing and detecting a fingerprint of a traditional Chinese medicine composition, and specifically relates to a method for establishing and detecting an HPLC fingerprint of a traditional Chinese medicine composition whose raw materials include spinach seeds and gardenia. The method comprises the following steps: preparing a sample of the traditional Chinese medicine composition, accurately weighing it, adding an extraction solvent, ultrasonically treating it, cooling it, centrifuging it, and then filtering it with suction; taking the filtrate and spinning it to obtain a residue; adding a solvent to dissolve the residue, fixing the volume, shaking it well, filtering it, and taking a subsequent filtrate to obtain a sample solution; preparing a reference solution: taking six reference substances, including genipin gentiobioside, gardeniaside, magnolamine, and spinosin, to prepare a mixed reference solution; and using HPLC to perform analysis and determination to complete the construction of a fingerprint. The method has strong specificity, high precision, good stability, and strong repeatability, and provides richer information for the intrinsic quality control of traditional Chinese medicine compositions whose raw materials include spinach seeds and gardenia.

The present invention relates to the technical field of drug analysis, and specifically relates to an analytical method for Litsea lancilimba formula granules. The Litsea lancilimba formula granules are formed by decocting Litsea lancilimba decoction pieces in water while collecting volatile oil and carrying out concentration, drying, and granulation. The analytical method comprises the following items: descriptions, identification, tests, extractives, characteristic chromatographic fingerprints, and assay. The identification method uses thin-layer chromatography for identification; in the characteristic chromatographic fingerprints, by taking the peak corresponding to the reference peak of magnoflorine reference substance as an S peak, high performance liquid chromatography is used to mark four characteristic peaks; and the assay is to determine the content of magnoflorine. The analytical method established in the present invention improves the analytical requirements for medicinal materials, decoction pieces, standard decoctions and formula granules of Litsea lancilimba, which helps to establish a perfect quality control system for various products of Litsea lancilimba, and helps to ensure the quality and clinical efficacy of the Litsea lancilimba formula granules.

This invention provides a method for constructing HPLC characteristic chromatograms of processed medicinal slices, standard decoctions, and formulated granules of *Sophora flavescens*, comprising: A) dissolving and extracting the test sample raw material using a solvent to obtain a test solution; B) determining the test solution using high-performance liquid chromatography (HPLC) to obtain HPLC characteristic chromatograms of processed medicinal slices, standard decoctions, and formulated granules of *Sophora flavescens*; the HPLC chromatographic conditions are as follows: a C18 column; mobile phase A is acetonitrile solution, mobile phase B is 0.05% trifluoroacetic acid aqueous solution, and gradient elution is performed; specifically, the gradient elution is as follows: 0–15 min, phase A: 5%–11%, phase B: 95%–89%; 15–40 min, phase A: 11%–18%, phase B: 89%–82%; 40–60 min, phase A: 18%–21%, phase B: 82%–79%. This invention employs high-performance liquid chromatography (HPLC) with acetonitrile-0.05% trifluoroacetic acid solution as the mobile phase for gradient elution. Using magnoflorine, isocoryne, styraxine, and styraxine as references, a method for establishing characteristic HPLC chromatograms of processed slices, standard decoctions, and formulated granules of *Sophora flavescens* was developed. This provides more scientific technical means for controlling the medicinal quality of processed slices, standard decoctions, and formulated granules of *Sophora flavescens*.

The invention provides a preparation method and application of a spina date seed extract. Comprising the following steps: 1, extracting; and step 2, purification. The invention also relates to application of the spina date seed extract in preparation of drugs for improving and / or treating depression and anxiety. According to the adsorption and analysis characteristics of the macroporous adsorption resin on the magnoflorine components in the spina date seeds, the conditions for purifying the magnoflorine components in the spina date seeds are determined, and theoretical basis and technical support are provided for preparing the magnoflorine components in the spina date seeds and realizing industrial production. According to the method, scientific technologies such as activity tracking, separation and purification and the like are combined on the basis of a traditional method, and a strategy of accurately positioning and enriching a core functional substance group becomes a successful example of modern research of traditional Chinese medicines; the substance obtained by the preparation method not only retains the concept of synergistic effect of multiple components of traditional Chinese medicines, but also greatly improves the effectiveness and controllability of the medicine through enrichment, and lays a foundation for developing and preparing the medicine for improving and treating depression and anxiety disorder.

The invention relates to construction of a Qingbai Tongbi capsule fingerprint spectrum and application of the Qingbai Tongbi capsule fingerprint spectrum in drug quality control. The HPLC fingerprint spectrum of the Qingbai Tongbi capsule has 24 characteristic fingerprint chromatographic peaks, wherein the peak 4 is catechin, the peak 10 is sinomenine, the peak 12 is gallic acid, the peak 13 is magnoflorine, the peak 14 is paeoniflorin, the peak 17 is liquiritin, the peak 18 is 1, 2, 3, 4, 6-O-pentagalloylglucose, and the peak 24 is ammonium glycyrrhizinate.

The invention discloses a method for simultaneously determining seven mass markers (Q-Marker) in serum of traditional Chinese medicine particles for treating cervical pain, which is characterized in that the seven mass markers are magnoflorine, puerarin, tetrahydropalmatine, paeoniflorin, notoginsenoside R1, ligustilide and decuricin; the method comprises the following steps: adding a serum sample into acetonitrile and acetone, carrying out vortex and centrifugation, taking a supernatant, loading the supernatant into a Hybrid SPEcolumn, washing with water to remove salt and polar interferents, eluting a target analyte with acetonitrile, filtering the eluent through a microfiltration membrane, carrying out sample introduction determination, and carrying out quantitative analysis by adopting a high performance liquid chromatography-mass spectrometry method and an electrospray ion source. And quantitative analysis is carried out in a positive / negative ion multi-reaction monitoring detection mode. The method is efficient, sensitive and high in selectivity, and solves the problems of serious matrix interference, indefinite target components, difficulty in simultaneous analysis of multiple components and the like in the prior art.

The invention discloses a method for producing aporphine alkaloid and recombinant bacteria used by the method. According to the invention, the synthetic route of the aporphine alkaloid is divided into four modules for research, and the de novo synthesis of the aporphine alkaloid is realized through various strategies; wherein the original strain is an IMX581 yeast strain. Experiments prove that the engineering strain constructed by the invention can be used for efficiently producing aporphine alkaloids such as (R)-croflorin, (R)-glaziovine, (S)-glaziovine, magnoflorin and the like. The aporphine alkaloids disclosed by the invention can be used for efficiently producing aporphine alkaloids such as (R)-croflorin, (R)-glaziovine, (S)-glaziovine, magnoflorin and the like. The method has an important application value.

The extraction method of effective components of traditional Chinese medicine composition for treating knee osteoarthritis based on entropy weight method and response surface method belongs to the technical field of traditional Chinese medicine extraction, establishes the content determination method of index components in the composition, i.e., sinomenine, magnoflorine, paeonol glycoside, paeoniflorin and dioscin, and carries out methodology investigation; the comprehensive score of index component content is calculated by the entropy weight method as an evaluation index, the influence factors are ethanol concentration, solid-liquid ratio and soaking time, the experiment is designed by applying the response surface method, the index weight is determined by the comprehensive weighting theory of evaluation index, the comprehensive score is calculated, the multiple quadratic regression model of comprehensive score and investigation factors is established, the variance analysis is carried out, the optimal process is predicted and verified, and the optimized extraction process parameters of Jiantongkang are determined. The extraction condition optimization research of index components of the composition based on the entropy weight method and the response surface method is strong in pertinence, simple in operation, high in accuracy, scientific and reasonable in process, and is suitable for the content determination of medicinal material effective components.

The invention provides a long-acting bactericide for plants. The long-acting bactericide comprises the following components in percentage by weight: 0.5-3% of a radix stemonae diphyllae extracting solution; 0.5-3% of a calendula extracting solution; 0.5-3% of a myrrh extracting solution; 0.5-3% of a cortex phellodendri extracting solution; 0.05 to 0.5 percent of a cationic surface active agent; 87.5% to 97.95% of water; according to the plant long-acting bactericide provided by the invention, alkaloid contained in the radix stemonae, berberine and magnoflorine in the cortex phellodendri, polysaccharide amino acid in the radix stemonae and gallic acid in the myrrh can form a stable acid-base association body, and the acid-base association body jointly acts on bacteria and viruses, so that the killing effect on the bacteria and the viruses is realized; the cationic surface active agent has remarkable dispersing and compatibilizing effects on flavone and resin in the natural plant extract, a stable clear solution can be formed, and meanwhile, R4-N + in the cationic surface active agent can also form a stable association body for alpha-gallic acid and beta-gallic acid brass to jointly act on bacteria and viruses.

This invention relates to a method for constructing the characteristic chromatogram of the traditional Chinese medicine Zhikang Capsules, as well as the chromatogram and its application. The method involves gradient elution using high-performance liquid chromatography (HPLC) to determine the contents of catechins, magnoflorine, berberine hydrochloride, and 1,4-bis[4-(glucoseoxy)benzyl]-2-isobutyl malate, thus constructing the characteristic HPLC chromatogram of Zhikang Capsules. Nine characteristic peaks were identified as the characteristic chromatogram, covering major components such as alkaloids, phenols, anthraquinones, and glycosides. A quality evaluation system for Zhikang Capsules was constructed. This method exhibits good specificity, precision, repeatability, and solution stability, and can be used to standardize the production of Zhikang Capsules and provide comprehensive quality control to ensure the uniformity and stability of the product's intrinsic quality.

The invention belongs to the technical field of medicines, and finds that aporphine alkaloids, especially magnoline and nuciferine, can reduce the BUN level and SCr level of a cisplatin-induced acute kidney injury model mouse through a large number of experimental screening, can obviously relieve cisplatin-induced kidney function impairment, and can be used for preparing medicines for treating acute kidney injury. The compound can be used as a medicine for reducing adverse reaction of renal function impairment caused by cis-platinum.

The invention discloses a kadsura pepper stem medicinal material detection method, which comprises: taking a kadsura pepper stem medicinal material test solution and a reference substance solution for detection, the reference substances comprise magnoflorine, sinomenine and tetrandrine, and the chromatographic conditions of the detection are that: a T3 chromatographic column is adopted, a T3 chromatographic column is adopted, a T3 chromatographic column is adopted, a T3 chromatographic column is adopted, a T3 chromatographic column is adopted, and a T3 chromatographic column is adopted; the mobile phase A is an acid aqueous solution, an alkali aqueous solution and / or a buffer salt aqueous solution, the mobile phase B is one or more of acetonitrile, methanol and tetrahydrofuran, the flow velocity is 0.1-1.0 ml / min, the column temperature is 20-40 DEG C, the detection wavelength is 200-400 nm, and the sample size is 1-10 [mu] l; and obtaining component information or component information and content information of the kadsura pepper stem medicinal material according to a detection result. The method disclosed by the invention is simple, convenient, efficient and rapid, and provides a theoretical basis for the quality control of the kadsura pepper stem medicinal material and the deep research of the pharmacodynamic material basis through the detection of the kadsura pepper stem medicinal material.

The application belongs to the technical field of traditional Chinese medicine detection, and specifically provides a construction method and a quality detection method for characteristic chromatograms of Sarcandra glabra medicinal preparation, wherein octadecylsilane bonded silica gel is used as a filler, acetonitrile is used as mobile phase A, and a water solution containing potassium dihydrogen phosphate is used as mobile phase B, a gradient elution program is continuously optimized, the column temperature is controlled to be 23-27 DEG C, 11 common characteristic peaks are finally obtained, 9 common characteristic peaks are effectively separated, the characteristic chromatogram obtained has a smooth baseline, the characteristic peaks have good peak shapes, and the detection time is short, thereby providing a scientific basis for comprehensively establishing the quality control standard of Sarcandra glabra formula granules. Moreover, the peak positions of tryptophan, syringin, isovanillin and magnoflorine can be accurately positioned, the known information of the characteristic peaks is increased, and the integrity and characteristics of Sarcandra glabra medicinal preparation (for example, formula granules and other preparations) are fully reflected.

The application belongs to the technical field of natural medicine chemistry, and particularly relates to a method for extracting compounds in Magnolia officinalis by using a low eutectic solvent and a detection method. Magnolia officinalis powder and a low eutectic solvent are mixed, and then soaking and ultrasonic extraction are sequentially performed to obtain an extraction liquid containing target compounds; the target compounds are magnoliabine, syringin, magnolol and honokiol. The low eutectic solvent is used to assist ultrasonic extraction of the Magnolia officinalis powder, and strong hydrogen bond interaction is formed between the low eutectic solvent and the target compounds, which is beneficial to the extraction of the target compounds, so that the extraction rate of the four target compounds in the Magnolia officinalis powder is improved.

The invention belongs to the technical field of medicine detection, and particularly relates to a multi-component content determination method of four-ingredient Tibetan cat milk soup. According to the method disclosed by the invention, the four-ingredient Tibet cat milk soup is determined by using specific high performance liquid chromatography conditions, so that complete separation of 13 components including gallic acid, loganic acid, gentiopicroside, magnoflorine, chebulagic acid, chebulagic acid, jateorhizine, berberine hydrochloride, quercetin, naringenin, apigenin, kaempferol and rhamnocitrin is realized; the quality condition of the four-ingredient Tibetan cat milk soup can be rapidly, simply, accurately and reliably known, the purpose of controlling the quality of the four-ingredient Tibetan cat milk soup by measuring the content of the 13 chemical components is achieved, and reference is provided for development and quality standard formulation of the four-ingredient Tibetan cat milk soup of the classic famous formula of Tibetan medicine.

The present invention discloses a series of novel amphiphilic quaternary ammonium salt magnoflorine analogues, their preparation methods and antibacterial applications. This series of compounds uses magnoflorine analogues as substrates, and hydrophilic quaternary ammonium salt fragments are introduced onto the phenolic hydroxyl groups through nucleophilic substitution reactions, thereby preparing a series of amphiphilic quaternary ammonium salt magnoflorine analogues, and their structural general formula is as follows. Compared with the existing magnoflorine analogues, the amphiphilic quaternary ammonium salt magnoflorine analogues prepared in the present invention have excellent antibacterial effects, especially have good antibacterial effects on Staphylococcus aureus ATCC 29213 and various methicillin-resistant Staphylococcus aureus (MRSA). At the same time, their water solubility is improved, their biological toxicity is reduced, and they have a high yield, and are expected to be further developed into semi-synthetic antibacterial drugs in clinical practice.

The present application belongs to the technical field of medicine detection, and particularly relates to a detection method of large-fruit Litsea cubeba formula granules. The large-fruit Litsea cubeba formula granules are prepared by decocting large-fruit Litsea cubeba decoction pieces with water, collecting volatile oil, concentrating, drying and granulating. The detection method comprises the items of appearance, identification, examination, extract, characteristic spectrum and content determination. The identification method is identification by thin layer chromatography. The characteristic spectrum is obtained by high performance liquid chromatography, and four characteristic peaks are indicated with the peak corresponding to the reference peak of magnoflorine as S peak. The content determination is detection of the content of magnoflorine. The detection method established by the present application improves the detection requirements of large-fruit Litsea cubeba medicinal materials, decoction pieces, standard decoction and formula granules, and is helpful to establish a perfect quality control system of large-fruit Litsea cubeba products, and to guarantee the quality and clinical efficacy of large-fruit Litsea cubeba formula granules.