Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Use and preparation method of magnoflorine in preparation of medicament for preventing and treating insomnia and depression

A technology for magnolia alkaloids and depression, applied in the fields of health care products and medicine, can solve the problems of time-consuming and laborious separation of active components of jujube seed, large dosage of traditional Chinese medicine, many side reactions, etc., and achieves large preparation amount, easy operation and loss of loss. less effect

Inactive Publication Date: 2018-10-12
TIANJIN MEDICAL UNIV
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of alkaloid monomer component Magnolin and its application, to solve the problem of many side effects of antidepressant and insomnia drugs in the prior art, and the problem of large dosage of traditional Chinese medicine
[0005] The second object of the present invention is to provide a method for preparing magnolialine by high-speed countercurrent chromatography, which can extract pure magnolialine from the Chinese jujube seed medicinal material, so as to overcome the time-consuming and labor-intensive extraction and separation of the active components of the wild jujube kernel in the prior art, and the relative fixation The sample has irreversible adsorption and the problem of low yield

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use and preparation method of magnoflorine in preparation of medicament for preventing and treating insomnia and depression
  • Use and preparation method of magnoflorine in preparation of medicament for preventing and treating insomnia and depression
  • Use and preparation method of magnoflorine in preparation of medicament for preventing and treating insomnia and depression

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation method of Magnolin

[0028] (1) Preparation of crude extract:

[0029] Grind 250g jujube kernels, pass through a 40-mesh sieve, degrease with petroleum ether, add 1200ml, 800ml, 600ml of 70% ethanol aqueous solution, heat and reflux for extraction 3 times, each time for 1 hour, filter, and combine the extracts , the filtrate is concentrated with ethanol until it has no alcohol smell, then dissolved with 2 times the amount of water, extracted 5 times with n-butanol, the extract is concentrated under reduced pressure, and the solvent is recovered to obtain the n-butanol crude extract, which is the sample to be separated. figure 1 shown.

[0030] (2) Adopt high-speed countercurrent chromatography to separate magnolialine and spinosin, 6 "'-feruloylspinosin:

[0031] Take n-butanol, ethyl acetate and water at a volume ratio of 2:3:5, place them in a separatory funnel to prepare a two-phase solvent system, take the upper phase as the stationary phase, and the l...

Embodiment 2

[0036] Pharmacodynamic experiment of magnolanine on CUMS depression and insomnia mouse model

[0037] 1 Experimental materials

[0038] 1.1 Experimental animals

[0039]Healthy male ICR mice (weight 20±2 g) were purchased from the Experimental Animal Center of the Institute of Radiation Medicine, Chinese Academy of Medical Sciences; they were reared for 3 days before the experiment to adapt to the environment. The room temperature is 22±2℃, and the relative humidity is 65%~70%. 12 hours of sunlight, free food and water. 1.2 Reagents and medicines:

[0040] Magnolialine, Spinosin (made in our laboratory); Venlafaxine Hydrochloride Sustained-Release Capsules (Chengdu Kanghong Pharmaceutical Group Co., Ltd.)

[0041] 1.3 Instruments

[0042] Stopwatch (Hoyer-Olidas, Switzerland);

[0043] AUX 120 analytical balance (Shimadzu (Hong Kong) Co., Ltd.);

[0044] ZH-YLS-1A Animal Autonomous Activity Recorder (Anhui Zhenghua Biological Instrument Equipment Co., Ltd.);

[0045] K...

Embodiment 3

[0094] Magnolialine is added with pharmaceutically acceptable excipients to prepare various dosage forms by conventional methods, such as liquid injections of various specifications, powder injections, emulsions for injection, tablets, pills, capsules, ointments, creams, patches, Liniments, powders, sprays, implants, drops, suppositories, ointments, candies, etc.

[0095] The administration route of magnolidine includes various administration routes: oral administration, injection administration, implant administration, intracavitary administration, sublingual administration, anal administration, transdermal administration, internal and external application, etc.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a use and preparation method of magnoflorine in preparation of a medicament for preventing and treating insomnia and depression. The preparation method utilizes high-speed countercurrent chromatography to separate magnoflorine. Compared with the prior art, the method for separating and purifying an alkaloid monomer magnoflorine has high monomer preparation and purificationefficiency, a large preparation amount, a low loss, high product purity, simple operation, economy and environmental friendliness. The high performance liquid chromatography (HPLC) detection result shows that the alkaloid monomer magnoflorine separated and purified through one step has purity of 95% or more. The animal pharmacodynamic experimental result shows that the magnoflorine can significantly improve and treat insomnia and depression.

Description

technical field [0001] The invention relates to a medicine and health product field, and relates to the application and preparation method of magnolanine, an active ingredient of alkaloids, in the treatment of depression and insomnia. Background technique [0002] Depression is a syndrome characterized by significant and persistent depression as the main clinical manifestation. In severe cases, suicide and self-mutilation will occur. At present, the global incidence of depression has reached 3.3%, while in developed countries it exceeds 6%. There are more than 700 million people with depression worldwide, and the prevalence of depression in my country is between 3% and 5%. With the development of society, this figure is still rising. Insomnia refers to the inability to sleep normally. The specific symptoms are tossing and turning, poor sleep quality, and reduced sleep time. Severe cases may cause insomnia all night [3] . In today's society, more and more people suffer from ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/473C07D221/18A61P25/24A61P25/20
CPCA61K31/473A61P25/20A61P25/24C07D221/18
Inventor 乔卫
Owner TIANJIN MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com