626 results about "Stationary phase" patented technology

The invention discloses an organic sample analysis method based on micro gas chromatography and a chromatographic analyzer, particularly relates to the technical field of micro gas chromatography, and is used for solving the problems of unstable separation performance and low complex sample detection precision caused by insufficient uniformity of a stationary phase coating of an existing micro chromatographic column. The method comprises the following steps: constructing a stationary phase distribution initial profile by continuously pre-charging carrier gas, alternately applying segmented temperature gradient, micro-negative pressure and directional pulse flow to remodel a uniform distribution layer, generating a correction factor set of a temperature-resistance coupling relationship by combining a correction mixture and a tracer gas pulse, dynamically adjusting a carrier gas flow velocity and a temperature program to generate a matched operation curve, and finally, carrying out dynamic calibration. High-precision separation detection and quantitative analysis are achieved, stable separation performance is maintained in a miniaturized structure by cooperatively controlling stationary phase distribution optimization and real-time parameter feedback correction, and the detection precision and reproducibility of a wide-boiling-range organic mixture are improved.

The invention discloses a fruit and vegetable exosome extraction method based on ligand fishing. The fruit and vegetable exosome extraction method comprises the following steps: suspending amino-functionalized Fe3O4 (at) SiO2 nanoparticles in a glutaraldehyde solution, and adding activated agglutinin to obtain Fe3O4 (at) SiO2-agglutinin nanoparticles; adding a glutaraldehyde solution into agarose gel to obtain a size exclusion chromatographic column filler; the method comprises the following steps: dispersing Fe3O4 (at) SiO2-lectin nanoparticles in a PBS buffer solution to obtain a uniform suspension, adding the uniform suspension into a size exclusion chromatographic column filler to obtain a uniform slurry, filling the size exclusion chromatographic column with the uniform slurry, and flushing with the buffer solution to form a magnetic bead stationary phase; and loading fruit and vegetable supernatant into a size exclusion chromatographic column, passing through the magnetic bead stationary phase, cleaning residual impurities combined on the exosome-magnetic bead compound by using a buffer solution, and dissociating the exosome from the magnetic beads by using a competitive eluent of the exosome to obtain the high-purity fruit and vegetable exosome. The problem that an existing exosome extraction method is difficult to meet the requirements of high efficiency, simplicity, high purity and high activity at the same time is solved.

The invention discloses a preparation method and application of a color master batch, belongs to the technical field of color master batch processing, and is used for solving the technical problem that the dispersity, color fastness and antistatic property of the color master batch in the prior art need to be further improved. The preparation method comprises the following steps: adding a modified pigment dispersion, modified polyurethane, polylactic acid and an auxiliary additive into a double-screw extruder, and carrying out melt extrusion and grain-sized dicing to obtain the color master batch. The modified polyurethane is used as a switch phase, the polylactic acid is used as a stationary phase, and the modified pigment dispersion and the auxiliary additive are used as assistants, and melt extrusion is carried out to obtain the color master batch. The antistatic property and color fastness of the color master batch are improved, and the mechanical property and dispersity of the color master batch are also improved.

The invention provides a stationary phase for DNA synthesis and a preparation method and application thereof. The preparation method of the stationary phase for DNA synthesis, provided by the invention, comprises the following steps: S1, preparing nano magnetic ferroferric oxide particles, and coating the surfaces of the nano magnetic ferroferric oxide particles with SiO2 layers to obtain a nano magnetic composite material Fe3O4-SiO2; s2, carrying out azide group modification on the surface of the nano magnetic composite material Fe3O4 (at) SiO2 to form a surface-modified nano magnetic composite material Fe3O4 (at) SiO2 (at) N3; and S3, loading the initiator to the surface of the surface-modified nano magnetic composite material Fe3O4 (at) SiO2 (at) N3 through Click reaction to obtain the stationary phase for DNA synthesis. The stationary phase provided by the invention can be used for DNA synthesis, and has the characteristics of high bonding strength, good stability, difficult shedding of initiators, high DNA synthesis yield and the like.

The invention discloses a construction method and an identification method of characteristic chromatograms of a senecio scandens medicinal material as well as decoction pieces, standard decoction and formula granules thereof, and relates to the technical field of traditional Chinese medicine identification. The construction method of the specific chromatogram comprises the following steps: extracting a to-be-detected sample with methanol to obtain a test solution; the method comprises the following steps: dissolving 5-caffeoylquinic acid, chlorogenic acid, caffeic acid, 4-caffeoylquinic acid, rutin, hyperoside, isoquercitrin and 4, 5-dicaffeoylquinic acid with methanol to obtain a reference solution; and a liquid chromatograph is adopted for determination. A chromatographic column of the liquid chromatograph takes phenyl-methyl polysiloxane as a stationary phase, acetonitrile as a mobile phase A and 0.05 vol%-0.3 vol% formic acid aqueous solution as a mobile phase B for gradient elution. The information of the characteristic chromatogram constructed by the method is rich, and the quality of the senecio scandens medicinal material, decoction pieces, standard decoction and formula granules can be quickly and comprehensively reflected.

The invention discloses a recyclable biochip stationary phase as well as a preparation method and application thereof, and belongs to the field of biochips. The preparation method of the biochip stationary phase comprises the following steps: (1) activating a glass slide by adopting plasma, and then putting the glass slide into a silane coupling agent solution to react, so that a silane coupling agent is covalently bound with the glass slide to obtain a functionally modified biochip; and (2) combining the 5'end modified single chain oligo with the functionally modified biochip to obtain the biochip stationary phase. The biochip stationary phase disclosed by the invention is low in cost, excellent in physicochemical property, good in stability and tolerance and capable of adapting to a complex environment; more importantly, the method can be recycled, the resource utilization rate is increased, the pollution is reduced, and the method has huge application potential in the leading technical field of DNA synthesis by an enzyme method and the like.

The invention discloses a strong-hydrophobicity chromatographic stationary phase modified by a covalent triazine skeleton containing an octyl chain as well as a preparation method and application of the strong-hydrophobicity chromatographic stationary phase. Activated silica gel is modified by utilizing a silane coupling agent, so that gamma-glycidyl ether oxypropyl trimethoxy is introduced into the surface of the silica gel; and performing in-situ growth of a covalent triazine skeleton layer containing an octyl chain through a Schiff base condensation reaction to prepare the strong-hydrophobicity chromatographic stationary phase. A covalent triazine skeleton containing a hydrophobic octyl chain in the structure is used for modifying the surface of silica gel, and a bonding phase contains a triazine ring, a benzene ring, an octyl chain and an amino group. According to the stationary phase, a hydrophobic long octyl chain is introduced into a bonding unit of CTF, so that the structure, morphology and performance of the CTF are changed, a'flower-on-sphere 'morphology is formed, and the stationary phase has a rigid porous structure, adjustable hydrophilicity / hydrophobicity, a rich pi electron system, strong hydrophobicity and good separation selectivity for non-polar compounds, and can be widely applied to separation of various samples.

The invention relates to a hydrophilic core-shell type chromatographic stationary phase material and application thereof. The hydrophilic core-shell type chromatographic stationary phase material comprises spherical silicon dioxide and a mesoporous layer which coats the spherical silicon dioxide, contains an amide group and is compounded with silicon dioxide. The preparation method comprises the following steps: firstly preparing solid silicon dioxide microspheres, then coating the surfaces of the silicon dioxide microspheres with mesoporous silicon dioxide shell layers through surface in-situ growth, and finally modifying the mesoporous shell layers through grafting and polymerization reaction to obtain the hydrophilic core-shell material containing the amide groups. The aperture of the material is mesoporous 2-20 nanometers, hydrophilic oligosaccharide substances can be separated, radix morindae officinalis oligosaccharide can be separated, and compared with a traditional chromatographic column, the separation degree is better, the column efficiency is higher, and the good separation performance is achieved. The preparation steps are simple, the reaction conditions are mild, and the method is simple.

The invention discloses a polymer stationary phase carrier for DNA synthesis as well as a preparation method and application of the polymer stationary phase carrier. The polymer stationary phase carrier comprises a polymer substrate, and the surface of the polymer substrate is provided with active functional groups such as ester groups, carboxyl groups, halogen or hydroxyl groups, so that the polymer substrate can be modified; the surface of the polymer substrate is modified with a first functional group, the first functional group is covalently bonded with the polymer substrate, and the first functional group is a functional group capable of reacting with an ester group, a carboxyl group or halogen; the surface of the polymer substrate is also modified with a functional group, and the functional group is a group capable of generating a click reaction; the polymer stationary phase carrier further comprises an initiation chain coupled to the polymer substrate through the functional group; and the initiating chain is coupled with the surface of the polymer substrate with the modified functional group through the second functional group. The stationary phase carrier disclosed by the invention can be used for carrying out a biological DNA synthesis reaction, and has relatively high yield and accuracy.

The invention provides a method for separating EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) through supercritical fluid chromatography, and relates to the technical field of separation and purification. The invention provides a method for separating EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) by supercritical fluid chromatography, which comprises the following steps: by taking supercritical carbon dioxide and an organic reagent as mobile phases, and by utilizing the specific mobile phase property of supercritical CO2 after being mixed with the organic reagent (saturated aliphatic hydrocarbon) and matching with a normal-phase chromatographic column, separating the EPA from the DHA by supercritical fluid chromatography. A specific stationary phase (one or more of a silica gel surface bonded propylurea stationary phase, a silica gel surface bonded phenyl stationary phase and a silica gel surface bonded imidazolyl stationary phase) is adopted, effective separation and preparation of the EPA and the DHA in the fish oil can be achieved, the EPA and the DHA in the fish oil can be effectively separated within 50 min, the separation speed is high, the separation effect is good, and the method is suitable for industrial production. The purity of the obtained EPA and DHA is 98% or above, the purity of the EPA and the DHA is high, and the EPA and the DHA have good application prospects in the fields of medicine production and food and health care products.

The invention provides a separation and detection method of a cis-isomer in olotinib. According to the method, chiral chromatographic column high performance liquid chromatography is adopted, cellulose derivative bonded silica gel is taken as a stationary phase, and n-hexane and ethanol are taken as a mobile phase. The method provided by the invention can effectively separate and measure the cis-isomer in the olotinib, and has the advantages of high efficiency, simplicity, convenience, high sensitivity, good separation effect and separation degree of 5.42. The invention provides a detection method for developing and marketing the olatinib and accurately detecting the cis-isomer in the olatinib, and ensures the product quality.

The invention relates to a core-shell type chromatographic stationary phase material. The core-shell type chromatographic stationary phase material comprises spherical silicon dioxide and a covalent organic framework which coats the spherical silicon dioxide and contains an amide group. The preparation method comprises the following steps: firstly preparing modified silicon dioxide microspheres, then coating the surfaces of the silicon dioxide microspheres with a covalent organic framework through surface in-situ growth, and finally modifying the self-polycondensation covalent organic framework through oxidation reaction to obtain the self-polycondensation covalent organic framework material containing amide groups. The pore diameter of the material is 5-15 nanometers of mesopores, hydrophilic oligosaccharide substances can be separated, radix morindae officinalis oligosaccharide can be separated, and the material has good separation performance. The preparation steps are simple, the reaction conditions are mild, the method is simple and convenient, and good preparation reproducibility and stability are achieved.

The invention provides a method for detecting related substances of bromo-n-butane, which adopts a gas chromatography detection method, and the chromatographic conditions are as follows: a chromatographic column is a DB-624 quartz capillary column, and the specification is 30m * 0.32 mm * 1.8 [mu] m; the column temperature is 40 DEG C; the stationary phase is 6% of cyanopropyl phenyl-94% of dimethyl polysiloxane; the temperature of a sample injector is 250 DEG C; the sample size is 1 [mu] L; the split ratio is 20: 1; the carrier gas is nitrogen; the flow rate of nitrogen is 2.1 mL / min; a detector is a hydrogen ion spark detector, and the temperature of the detector is 250 DEG C. The invention further provides application of the method. The method can be used for rapidly and accurately detecting the related substances of the n-butyl bromide, realizes effective separation of the n-butyl bromide and impurities thereof, and is of great significance in improving the effective quality of the n-butyl bromide, controlling the content of the impurities brought into the synthesis reaction of the bupivacaine hydrochloride by the starting material and improving the quality stability of the bupivacaine hydrochloride.

The invention discloses a method for detecting rafenasin related substances, which comprises the following steps: detecting by adopting a high performance liquid chromatography under the chromatographic conditions that a stationary phase is an octadecyl silane bonded silica gel chromatographic column, a mobile phase A is a phosphate buffer aqueous solution, a mobile phase B is methanol, and gradient elution is carried out; the gradient elution procedure is as follows: within 0-10 minutes, the volume fraction of the mobile phase A is gradually changed from 88-92% to 73-77%; within 10-43 min, the volume fraction of the mobile phase A is gradually changed from 73-77% to 38-42%; within 43-45 min, the volume fraction of the mobile phase A is gradually changed from 38-42% to 88-92%; within 45-50 min, the volume fraction of the mobile phase A is kept at 88-92%. According to the chromatographic conditions disclosed by the invention, effective separation between the main peak of the rafenasin and each impurity can be realized, the specificity is good, and the sensitivity is high.

The invention belongs to the technical field of chromatographic separation materials, and discloses a preparation method and application of a GM-8 nanosheet material. The preparation method of the material comprises the following steps: respectively dissolving Cu (NO3) 2.3 H2O and 1, 3, 6, 8-tetra (4-carboxybenzene) pyrene in an organic solvent, mixing, transferring into a reaction bottle, adding trifluoroacetic acid and pyridine, and uniformly mixing and stirring to obtain a reaction mixture; and heating the reaction mixture to react for 7-9 hours to obtain a blue precipitate, cooling the blue precipitate to room temperature, and washing and drying the blue precipitate to obtain the ultra-stable microporous GM-8 nanosheet material. The nanosheet GM-8 is used as a gas chromatography stationary phase, high-efficiency separation and trace detection of xylene isomers can be realized, and the chromatographic column still keeps excellent separation capacity after being stored in an environment with air humidity of 65-90% for 31 months. The capillary gas chromatographic column can realize high-efficiency separation of disubstituted benzene isomers such as xylene, the separation degree of m-xylene / p-xylene is up to 18.2, and a new solution is provided for high-efficiency separation of isomers with similar properties.

Gas chromatography (GC) relies on the interaction of vapor molecules with the stationary phase coated on the column inner wall to separate different vapor molecules. Here, graphene is integrated as the ultrathin and electrically tunable stationary phase within a GC column. Graphene has different interactions with different vapor molecules, which leads to separation of those vapor molecules. In addition, by configuring graphene into a field effect transistor (FET) design, the molecule-graphene interaction can be controlled or tuned by adjusting the graphene Fermi level through the FET gate voltage. As a result, the binding energy between graphene and adsorbed vapor molecules can be tuned and hence the elution time and / or elution order of vapor molecules can be changed.

The invention discloses a cyclodextrin bonded covalent organic framework chiral stationary phase for chiral amino acid separation and a preparation method of the cyclodextrin bonded covalent organic framework chiral stationary phase. The method comprises the following steps: modifying silica gel microspheres by using a silane coupling agent to obtain silanized silica gel microspheres, reacting the silanized silica gel microspheres with a covalent organic framework by using a one-pot method to prepare covalent organic framework modified silica gel microspheres, and carrying out a thiol-alkyne click strategy by using vulcanized cyclodextrin as a functional molecule to obtain the covalent organic framework modified silica gel microspheres. The cyclodextrin bonded covalent organic framework chiral stationary phase is prepared; the cyclodextrin bonded covalent organic framework has strong chiral recognition capability, a packed column of the cyclodextrin bonded covalent organic framework can realize baseline separation of 12 dansyl amino acids within 30 minutes in liquid chromatography, the separation degrees of valine, isoleucine and leucine respectively reach 5.017, 4.351 and 3.782, the preparation process is simple to operate, the reaction condition is mild, the cost is low, and the cyclodextrin bonded covalent organic framework is suitable for industrial production. The practical application prospect is realized.

The invention discloses a preparation method of a dehydroabietic acid cellulose ester (DCE) bonded silica gel stationary phase, which comprises the following steps: firstly, carboxylating the surface of a SiO2 microsphere with a core-shell structure, then carrying out acylating chlorination, and then preparing amphiphilic DCE coated SiO2 solid particles with DCE in a chemical bonding manner. The DCE-SiO2 solid particle has the pore diameter of 5-30nm and the specific surface area of 50-400m < 2 > / g, has the characteristics of intact particle shape, uniform particle size, high mechanical strength, high pressure resistance and the like, and can be used as a liquid chromatographic column filler. The preparation method is simple and convenient, the column pressure is low, the repeatability is good, the chemically modified DCE has a phenanthrene ring skeleton and has good selective separation performance on saponin drugs containing ring structures, and the application prospect in the pharmaceutical industry is wide.

Systems, methods, and cartridges taught herein improve chromatographic performance in electrospray systems that feature chromatographic columns having a conductive or semiconductive stationary phase by electrically connecting a fluid junction located upstream of the chromatographic column to a fluid union located downstream of the chromatographic column using an electrical conductor. The electrical conductor creates a voltage equipotential between a first end of the chromatographic column and a second end of the chromatographic column that neutralizes current flow through the chromatographic column. Accurate electrospray current measurements are enabled while post-column peak dispersion and repeatable retention time are achieved.

The invention belongs to the field of preparation of gas adsorption metal organic framework compounds, and discloses a krypton gas separation compound as well as a preparation method and application thereof. According to the invention, 1, 2-bis [2-methyl-3-thienyl-5-pyridine] perfluorocyclopentane is adopted to form the rigid metal organic framework material for the first time, and the rigid metal organic framework material is used as a stationary phase to be applied to gas adsorption and separation processes. According to the preparation method, expensive gas separation equipment does not need to be used, separation conditions are simple and convenient, meanwhile, pure and stable gas can be obtained, and the gas yield is high. The krypton gas separation compound prepared by the method is high in yield and purity, has good thermal stability, adsorbability and separation performance, and can be widely applied to separation and purification of industrial gas rich in krypton gas and nuclear waste gas.

A method for separating and purifying 4MP1 from a mixture containing 4MTP2 and 1-Hexene comprises the following steps: continuously introducing a solution in which 4MTP2, 1-Hexene and 4MP1 with any concentration are dissolved into a simulated moving bed chromatography system as a raw material solution, collecting a high-purity 4MP1 solution from raffinate of the simulated moving bed chromatography system by adopting a metal organic framework material as a stationary phase, and separating the high-purity 4MP1 solution from the raffinate of the simulated moving bed chromatography system. The extraction liquid is a solution containing impurities 4MTP2 and 1-Hexene. The invention provides a brand new method for separating 4MP1 from 4MTP2 and 1-Hexene, and a complete separation scheme is provided for the prior art; in the separation of high-concentration 4MP1, the relative purity of the separated and purified 4MP1 can reach gt; the recovery rate is gt; and in the separation of low-concentration 4MP1, the rectification level is also exceeded, and the method is more suitable for 4MP1 thermosensitive substances.

The invention discloses a stationary phase of a novel zwitterionic monomer containing a multi-charge structure as well as a preparation method and application of the stationary phase, and belongs to the technical field of hydrophilic interaction chromatography (HILIC) stationary phases. According to the method, multi-charge zwitterions are used as hydrophilic monomers, the multi-charge structure of the multi-charge zwitterions endows the monomers with higher hydrophilic performance, and the multi-charge zwitterions are immobilized on the surface of a matrix by utilizing an active free radical polymerization method, so that a series of novel super-hydrophilic HILIC stationary phases are finally prepared. The multi-charge zwitterionic monomer adopted by the invention not only has better hydrophilic performance and higher charge density, but also can efficiently, simply and conveniently realize matrix surface grafting and modification. The developed stationary phase is suitable for HILIC column chromatography separation analysis, and the HILIC chromatographic performance can be greatly improved; the method can also be applied to the fields of biological sample pretreatment, material science, marker clinical diagnosis and the like, and has high economic and social values.

A 3,5,5-trimethylhexanoic acid composition containing 3,5,5-trimethylhexanoic acid and t-butyl alcohol in which the t-butyl alcohol concentration in terms of toluene-d8 measured by gas chromatography-olfactometry / mass spectrometry under the conditions below exceeds 0 ppb by volume and is 200 ppb by volume or less is provided. The composition is excellent in sulfuric acid coloring evaluation,(Measurement Conditions)The 3,5,5-trimethylhexanoic acid composition is concentrated, andThe concentrated sample is measured by gas chromatography-olfactometry using a column using dimethylpolysiloxane as a stationary phase, and the structure of a measured component is analyzed with a mass spectrometry detector.

The present invention relates to development of stationary phases and preparation of extraction columns having substantially improved capacity (i.e., amount of material purified per single chromatographic run) for lab scale to industrial scale extraction chromatographic separation, from small scale to industrial scale, of rare earth elements (REEs) and the platinum group metals (PGMs). More specifically the invention relates to preparation of stationary phases and extraction columns for extraction of REEs or PGMs as a group from containing matrices of typical REE or PGM feedstock and separation and purification of individual REEs or PGMs from each other.

The invention relates to a chromatography concentration method and device for a macromolecular dilute sample, belongs to the field of molecular chromatography concentration, and overcomes the inconvenience of laboratory instrument operation. When the macromolecular dilute sample mobile phase and the dry hydrogel particle stationary phase generate directional relative movement, the mobile phase solvent on the two-phase interface is continuously absorbed by the relatively excessive stationary phase, so that macromolecular solute with the particle size larger than that of the hydrogel particles is continuously subjected to concentration polarization enrichment on the interface; the concentration of macromolecular solutes on the interface is submerged and diluted by continuous supplementation of the relatively excessive mobile phase solvent following the interface to the interface; after passing through a chromatography path with enough length, the concentration of the macromolecular solute on the continuously moving interface is close to or reaches a highest equilibrium value under the superposition of two effects of enrichment and dilution, and the initial interface component of the mobile phase collected at the outlet of the chromatography column is a macromolecular concentrated solution close to or with the highest concentration multiple. The invention also discloses a macromolecular dilute sample chromatography concentration device consisting of the dry hydrogel particle medium and the chromatographic column.

The invention belongs to the technical field of purification of polypeptide drugs, and particularly relates to a purification method of tirpotide, which comprises the following steps: (a) preparing a tirpotide crude product solution by using an acetonitrile aqueous solution; (b) carrying out primary purification on the crude product solution of the tilpotide by selecting a specific stationary phase, a mobile phase and an elution gradient to obtain a first fraction; (c) performing secondary purification on the first fraction by selecting a specific stationary phase, a mobile phase and an elution gradient to obtain a second fraction; and (d) carrying out nanofiltration concentration, salt exchange, filtration sterilization and freeze-drying on the second fraction to obtain a tiall peptide product. The purity of the product obtained through the purification method is up to 99.5% or above, and the total recovery rate of purification is up to 80% or above. According to the method, the single-needle elution period is only 35-40 minutes, so that the use of organic solvents in the purification process of the tilpotide is greatly reduced, the purification cost is reduced, and the method tends to the long-term development of environmental protection.

The present invention belongs to the technical field of purification of polypeptide drugs, and specifically relates to a method for purifying tilpotide, comprising: (a) preparing a crude tilpotide solution using an acetonitrile aqueous solution; (b) selecting a specific stationary phase, mobile phase, and elution gradient to purify the crude tilpotide solution for a first time to obtain a first fraction; (c) selecting a specific stationary phase, mobile phase, and elution gradient to purify the first fraction for a second time to obtain a second fraction; (d) performing nanofiltration concentration, salt exchange, filtration sterilization, and lyophilization on the second fraction to obtain the tilpotide product. The product obtained by the purification method of the present invention has a purity of more than 99.5%, and the total recovery rate of purification is more than 80%. The single-needle elution cycle of the present invention is only 35 to 40 minutes, which greatly reduces the use of organic solvents in the purification process of tilpotide, reduces purification costs, and is more environmentally friendly in the long term.

The invention provides ester group functionalized cyclotriveratrum hydrocarbon as well as a preparation method and application thereof, and relates to the technical field of gas chromatographic analys.According to the ester group functionalized cyclotriveratrum hydrocarbon and the preparation method and application thereof, a long alkyl chain and an ester group are introduced to the lower edge of cyclotriveratrum hydrocarbon, so that the property of the cyclotriveratrum hydrocarbon serving as a gas chromatographic stationary phase is improved; by introducing a non-polar alkyl chain and a polar ester group, the melting point of the cyclotriveratrum hydrocarbon can be reduced, the film-forming property and the thermal stability of the cyclotriveratrum hydrocarbon are improved, and the separation performance of the cyclotriveratrum hydrocarbon serving as a stationary phase of a capillary gas chromatographic column on an analyte is improved; the method can effectively separate methyl benzaldehyde isomers, bromobenzaldehyde isomers, nitrobenzaldehyde isomers, diethylbenzene isomers, benzene series, homologues, cis-trans isomers and multi-component complex mixtures.

The invention relates to a supercritical fluid chromatographic separation method of bakuchiol, which comprises the following steps: dispersing a crude product in a solvent to prepare a test sample; carrying out supercritical fluid chromatographic separation on the test sample; the supercritical fluid chromatographic separation conditions are as follows: a mobile phase is a mixture of supercritical carbon dioxide and C1-C3 alcohol compounds in a volume ratio of (99.5-98.5): (0.5-1.5), and isocratic elution is performed; the stationary phase of the chromatographic column is octadecylsilane chemically bonded silica. The supercritical fluid chromatographic separation method of the bakuchiol makes up the research blank of analyzing and separating the bakuchiol by adopting the supercritical fluid chromatography in the traditional method, and can realize accurate detection and effective preparation of the bakuchiol. Meanwhile, the separation method is short in time consumption and good in separation effect.

The invention provides an extraction and separation method of tantalum-niobium ore, and relates to the technical field of tantalum-niobium ore decomposition. The tantalum-niobium ore extraction and separation method comprises the steps that target tantalum-niobium ore is taken and added into an ammonium bifluoride solution to be heated and decomposed, and decomposition liquid is obtained after solid-liquid separation; and carrying out gradient elution on the decomposition liquid by taking ion exchange resin as a stationary phase and hydrochloric acid as a mobile phase to respectively obtain fluoroniobic acid, fluotantalic acid and residual liquid. The ammonium bifluoride solution is adopted to replace traditional high-concentration acid, so that the environmental pollution is reduced, the extraction efficiency and the resource recovery rate are improved, and the resource waste is reduced. Meanwhile, equipment corrosion is reduced, the service life of equipment is prolonged, cost is reduced, and economic benefits are improved. The gradient elution technology of the ion exchange resin simplifies the process and improves the product quality.