150 results about "Stationary phase" patented technology

A method for separating and purifying 4MP1 from a mixture containing 4MTP2 and 1-Hexene comprises the following steps: continuously introducing a solution in which 4MTP2, 1-Hexene and 4MP1 with any concentration are dissolved into a simulated moving bed chromatography system as a raw material solution, collecting a high-purity 4MP1 solution from raffinate of the simulated moving bed chromatography system by adopting a metal organic framework material as a stationary phase, and separating the high-purity 4MP1 solution from the raffinate of the simulated moving bed chromatography system. The extraction liquid is a solution containing impurities 4MTP2 and 1-Hexene. The invention provides a brand new method for separating 4MP1 from 4MTP2 and 1-Hexene, and a complete separation scheme is provided for the prior art; in the separation of high-concentration 4MP1, the relative purity of the separated and purified 4MP1 can reach gt; the recovery rate is gt; and in the separation of low-concentration 4MP1, the rectification level is also exceeded, and the method is more suitable for 4MP1 thermosensitive substances.

The invention discloses a stationary phase of a novel zwitterionic monomer containing a multi-charge structure as well as a preparation method and application of the stationary phase, and belongs to the technical field of hydrophilic interaction chromatography (HILIC) stationary phases. According to the method, multi-charge zwitterions are used as hydrophilic monomers, the multi-charge structure of the multi-charge zwitterions endows the monomers with higher hydrophilic performance, and the multi-charge zwitterions are immobilized on the surface of a matrix by utilizing an active free radical polymerization method, so that a series of novel super-hydrophilic HILIC stationary phases are finally prepared. The multi-charge zwitterionic monomer adopted by the invention not only has better hydrophilic performance and higher charge density, but also can efficiently, simply and conveniently realize matrix surface grafting and modification. The developed stationary phase is suitable for HILIC column chromatography separation analysis, and the HILIC chromatographic performance can be greatly improved; the method can also be applied to the fields of biological sample pretreatment, material science, marker clinical diagnosis and the like, and has high economic and social values.

This invention discloses an analytical method for benzothiazole contaminants, comprising the following steps: detection by high performance liquid chromatography (HPLC), with the following chromatographic conditions: a phenylhexyl column as the stationary phase, mobile phase A being water, mobile phase B being acetonitrile, and gradient elution; wherein the benzothiazole contaminants are at least one of 2-aminobenzothiazole, 2-hydroxybenzothiazole, benzothiazole, 2-methylbenzothiazole, 2-mercaptobenzothiazole, 2,5-dimethylbenzothiazole, 2-cyanobenzothiazole, ethyl 2-carboxylate benzothiazole, 2-chlorobenzothiazole, 2-bromobenzothiazole, 2-methylthiobenzothiazole, phenylthiocyanate, N-tert-butyl-2-benzothiazole sulfenamide, 2-(2-hydroxyphenyl)-benzothiazole, N-cyclohexyl-2-benzothiazole sulfinamide, dibenzothiazole disulfide, and N,N-dicyclohexyl-2-benzothiazole sulfonamide.

The invention discloses radioactive gas separation and purification equipment, a radioactive gas separation and purification method, a radioactive gas separation and purification medium and radioactive gas separation and purification equipment, and relates to the technical field of radioactive gas separation and purification. The radioactive gas separation and purification equipment comprises a first separation column, a second separation column, a carrier gas container, a quantitative structure, a chromatographic column, a detection assembly, a first control valve, a storage container, a temperature controller and a to-be-treated gas container. The carrier gas container is connected with the first control valve, and the to-be-treated gas container, the quantitative structure, the first control valve, the first separation column, the second separation column, the chromatographic column and the storage container are sequentially connected. The first separation column, the second separation column and the chromatographic column are all filled with stationary phases which are all used for purifying target substances in the to-be-treated gas based on different adsorption rates of the stationary phases relative to different substances. Therefore, due to the arrangement of the first separation column, the second separation column and other structures, large-volume ambient air or gaseous effluent can be purified, and subsequent radioactive substance concentration measurement is facilitated.

A chromatography system is provided. The chromatography system is configured to process a feed fluid containing a plurality of components, wherein at least one component of the plurality of components of the feed fluid is a target component. The chromatography system comprises: a flow path comprising a plurality of fluid control components configured to control a fluid flow; a stationary phase, wherein the stationary phase is at least one membrane adsorber connected to the flow path and the stationary phase is configured to isolate the target component. The flow path is configured such that harvesting of the target component is optimized.

The application discloses a kind of cell membrane chromatographic column based on biotin ligase directional covalent fixation and its preparation method and application, belong to chromatography science and technology field. By bonding 2-imino biotin to silica gel, then using biotin ligase specific recognition with Avi-Tag cell membrane fragments, and stable covalent bonding occurs with 2-imino biotin silica gel, so that cell membrane is fixed on the surface of silica gel to obtain Avi-Tag bridged cell membrane chromatographic stationary phase, finally using wet column packing, obtain cell membrane chromatographic column based on biotin ligase directional covalent fixation. The cell membrane chromatographic column can prolong the service life of cell membrane chromatographic column, improve the stability of cell membrane chromatographic column during use, and the short peptide tag (Avi-Tag) used can improve the non-specific adsorption and poor specificity of the cell membrane chromatographic column prepared based on protein tag technology to a certain extent, providing technical support for the wide application of cell membrane chromatographic column.

The present disclosure is directed to stationary phase materials for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified silica particle stationary phase materials, which are optionally also methoxy-terminated polyethylene glycol surface modified.

The invention discloses a method for detecting related substances in testosterone enanthate by high performance liquid chromatography. A mobile phase A and a mobile phase B are used as mixed mobile phases for gradient elution; the mobile phase A is water; the mobile phase B is acetonitrile; the volume ratio of the mobile phase A to the mobile phase B is (5%-70%): (30%-95%); a chromatographic column adopted by the high performance liquid chromatography method is Agilent ZORBAX Eclipse XDB-C8, and the detection method is characterized in that the detection method is simple and easy to implement. According to the method, the testosterone enanthate and the related substances thereof are separated and detected by screening a stationary phase chromatographic column and optimizing a mobile phase elution mode and an elution component ratio, and compared with a method in European Pharmacopoeia, after the chromatographic column is subjected to gradient elution, not only is the retention time of an impurity D prolonged, but also the quality of the impurity D is improved. And the separation degree of the impurity F and the impurity G is increased, so that the elution capacity and the separation degree among the impurities are enhanced.

The invention relates to the technical field of pharmaceutical analysis equipment, and discloses pharmaceutical active component quantitative analysis equipment which comprises a chromatographic column, the chromatographic column comprises a hollow column body, a filter screen is fixedly arranged at the lower end in the column body, a stationary phase is arranged above the filter screen, a piston is arranged above the stationary phase, and the piston is connected with a driving part; the piston comprises a first shell facing the stationary phase, a second shell is coaxially arranged on the side, away from the stationary phase, of the first shell in a sliding mode, an elastic supporting piece is arranged between the first shell and the second shell, a plurality of connecting holes are coaxially formed in the bottom of the first shell and the bottom of the second shell, liquid discharging columns are arranged in the connecting holes, and liquid inlets are formed in the tops of the liquid discharging columns. A liquid outlet is formed in the side wall of the bottom of each liquid discharging column, the multiple liquid discharging columns are connected with a driving mechanism, and the second shell is connected with a liquid inlet pipe. According to the invention, the state of a stationary phase bed layer can be actively adjusted, and uniform introduction and dispersion of liquid medicine are realized, so that the chromatographic separation efficiency and the quantitative analysis accuracy are improved.

The invention discloses a glucose-fructose efficient separation and high-purity fructose preparation method, and belongs to the technical field of monosaccharide purification, the preparation method adopts functional resin as a stationary phase and water as an eluent, and chromatographic separation is carried out at 55-65 DEG C; the method specifically comprises the following steps: S1, filling a chromatographic column with functional resin, and balancing with 55-65 DEG C water; s2, pumping a mixed sugar solution of glucose and fructose into a chromatographic column; s3, eluting with water at 55-65 DEG C, and respectively collecting a glucose fraction and a fructose fraction; and S4, respectively concentrating, crystallizing and drying the glucose fraction and the fructose fraction to obtain a high-purity fructose product. According to the separation method, the purity of the obtained glucose is 94.4%-95.1%, the recovery rate of the glucose is 97.1%-98.0%, the purity of the obtained fructose is 98.6%-99.2%, and the recovery rate of the obtained fructose is 95.5%-96.8%.

The application discloses a separation and detection method of methoxy PEG derivatives and methylsulfonyl PEG derivatives, which comprises the following steps: mixing a sample to be detected, ammonia and an ammonium salt catalyst, carrying out a derivatization reaction to obtain a derivative product, and detecting the derivative product by using a liquid chromatography method. The sample to be detected is a methoxy PEG derivative or a methylsulfonyl PEG derivative sample. In the method, the stationary phase of the chromatographic column is octadecylsilane bonded silica gel, the mobile phase A is a 0.01-5% formic acid aqueous solution in terms of volume percentage concentration, the mobile phase B is acetonitrile and methanol in a volume ratio of (1-5):1, a gradient elution program is adopted, and an electrospray detector is used. The method can effectively separate and quantitatively analyze the methoxy PEG derivatives and the methylsulfonyl PEG derivatives, and the method is completely in line with the standards and performs well in the quantitative limit, the linear range, the accuracy and the like, thereby providing an effective guarantee for subsequent product quality control or evaluation.

The invention discloses a method for detecting the content of formaldehyde in polyethylene glycol 400, and relates to the technical field of detection. The invention relates to a method for detecting the content of formaldehyde in polyethylene glycol 400, which comprises the following steps: S1, precisely weighing 4-(3-methylphenyl)-2H-pyridine [4, 3, e]-1, 2, 4-thiodiazine-1, 1-dioxo, and adding methanol to dissolve the 4-(3-methylphenyl)-2H-pyridine [4, 3, e]-1, 2, 4-thiodiazine-1, 1-dioxo to prepare a reference solution; s2, precisely weighing polyethylene glycol 400 and reactants to prepare a test solution; and S3, respectively detecting the test solution and the reference solution by adopting a high performance liquid chromatography, and calculating the content of formaldehyde in polyethylene glycol 400, wherein the chromatographic conditions of the high performance liquid chromatography are as follows: gradient elution is adopted; a chromatographic column is characterized in that octadecylsilane chemically bonded silica is used as a stationary phase; the mobile phase comprises a first mobile phase and a second mobile phase, the first mobile phase is a buffer solution, and the second mobile phase is an organic solvent. The method provided by the invention can accurately measure the content of formaldehyde in polyethylene glycol 400.

The utility model discloses a column chromatography separation device which comprises a chromatographic column and further comprises a pressurizing structure which is detachably connected with the top end of the chromatographic column and is used for pushing a mobile phase in the chromatographic column to move downwards so as to accelerate the separation process, the switch is arranged at the upper part of the chromatographic column or the lower part of the pressurizing structure and is used for connecting or disconnecting the pressurizing structure and the inner cavity of the chromatographic column; the blocking piece is arranged in the chromatographic column, is positioned at the bottom of the stationary phase and is used for preventing the stationary phase from losing. The column chromatography separation device disclosed by the utility model is simple in structure, convenient to use, high in separation speed, good in separation effect and low in use cost.

A process for the production of a membrane protein is provided. The process including the steps of: expressing a membrane protein in a host organism present in an aqueous medium, liberating the membrane protein from the host organism, adding a detergent solution to solubilize the membrane protein, recovering a liquid fraction of the solubilized membrane protein, subjecting the liquid fraction to chromatography to bind or retain the membrane protein on a stationary phase, and eluting the stationary phase with an elution buffer to produce the membrane protein. The process can produce relatively large amounts of membrane proteins in an efficient way without compromising the quality of the end product.

a kind of sp 2 C covalent organic framework functionalized silicon-based chromatographic stationary phases, their preparation methods, and applications. Preparation steps: (1) Preparation of SiO2-NH2: Aminated activated spherical silica particles are prepared using an amino-containing silane coupling agent; (2) SiO2@sp 2 C-COF preparation: Using TMT and TFPT as raw materials, sp was obtained through a Schiff base-mediated aldehyde-alcohol condensation reaction initiated by a self-assembled monolayer auxiliary surface. 2 A C covalent organic framework is loaded onto the surface of a silica matrix via C=N bonds. 2 C-COF possesses an in-plane π-conjugated, highly ordered, and robust framework structure. Its unique hydrophilic covalent triazine groups and hydrophobic benzene rings can provide various interactions such as hydrophobicity, π-π stacking, hydrogen bonding, and hydrophilicity. This enables the stationary relative shape-restricted and geometric isomers to exhibit special selectivity and separation capabilities, overcoming the instability of common imine-bonded COFs in liquid chromatography (HPLC) and the limited selectivity of relatively shape-restricted and geometric isomers stationed in traditional bonded silica gel.

This invention relates to a new stationary phase carrying functional groups comprising a halogen substituted aromatic ring. Target molecules can interact with this stationary phase by halogen bonding. The stationary phase is suitable for SPE or chromatographic separations.

The invention belongs to the technical field of high performance liquid chromatography, and particularly relates to a preparation method and application of chiral hybrid microspheres containing beta-cyclodextrin shells. Firstly, (3-mercaptopropyl) triethoxy silane and vinyl cyclodextrin are used as raw materials, and a beta-cyclodextrin derivative modified by triethoxy silane is generated in a mercapto-alkene click chemistry mode. And reacting the beta-cyclodextrin with tetraethyl silicate and solid silicon dioxide to obtain a core-shell hybrid microsphere containing a beta-cyclodextrin shell layer. The beta-cyclodextrin can be used as a chiral selective agent and has a chiral recognition function, so that the hybrid microsphere can be used as a chiral chromatographic stationary phase and is used for separation and analysis of chiral enantiomers, and compared with chiral hybrid microspheres containing beta-cyclodextrin shells in the prior art, the loading capacity and firmness degree of the beta-cyclodextrin on the surface of a matrix are improved; the chiral separation capability of the chiral hybrid microspheres is improved.

This application belongs to the field of seed detection and analysis technology, specifically relating to a method for constructing a characteristic chromatogram of Isatis indigotica seeds and a method for detecting Isatis indigotica seeds. The construction method includes the following steps: providing a reference solution; preparing a test solution using Isatis indigotica seeds; detecting the reference solution and the test solution using ultra-high performance liquid chromatography (UHPLC); importing the chromatograms obtained from detecting the reference solution and the test solution into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system to establish a characteristic chromatogram of Isatis indigotica seeds; the reference solution contains alkaloids as a reference standard; the UHPLC method meets the following conditions: the stationary phase includes a C18 column; the mobile phase includes mobile phase A and mobile phase B, mobile phase A including methanol and / or mobile phase B including water; and a gradient elution program is used. This application uses UHPLC to construct a characteristic chromatogram reflecting the secondary metabolites of Isatis indigotica seeds, providing a novel reference for evaluating the quality of Isatis indigotica seeds.

The invention discloses a preparation method of a high-capacity cation chromatographic stationary phase, which comprises the following steps: preparing non-crosslinked PS (polystyrene) seed microspheres by dispersion polymerization, dispersing the non-crosslinked PS seed microspheres in water under the action of a proper emulsifier to form a seed microsphere emulsion, swelling the seed microspheres at room temperature by a proper reagent to obtain the high-capacity cation chromatographic stationary phase. Then adding a mixed system of anhydride monomers, an initiator and a pore-foaming agent, continuing swelling for a certain time, and then heating to initiate the monomers to have a polymerization reaction; and after the reaction is finished, cleaning to remove the pore-foaming agent in the product microspheres, and finally, putting the cleaned polymer microspheres into hot water and stirring to promote hydrolysis of anhydride in pores, thereby obtaining the porous polymer microspheres with complete crosslinking function.

Provided is an amine separation method that includes separating an amine through chromatography using a stationary phase including a ligand having a crown ether-like cyclic structure and being supported on a carrier; and a mobile phase containing a salt of a cation and an acid anion at a concentration of 0.2 mM or more and 50.0 mM or less and containing a solvent having a water content of 50 vol. % or less.

The invention belongs to the technical field of pharmaceutical analysis, and discloses a liquid chromatography detection method of a pimobendan intermediate, which comprises the following steps: injecting octadecyl bond and silica gel as a stationary phase, a phosphate buffer solution with the pH value of 3.0-5.0 as a water phase and methanol (or acetonitrile) as an organic phase into a high performance liquid chromatograph under the conditions that the column temperature is 30-60 DEG C, the sample injection is 5-30 mu L and the flow velocity is 0.5-2 ml / min, and carrying out gradient elution to obtain the pimobendan intermediate. And the sample is analyzed in a wavelength range of 200-230nm. By adjusting the mobile phase system and optimizing the mobile phase gradient, a main peak and an impurity peak are completely separated, the analysis speed is high, the detection sensitivity is high, and the stability and the quality of a product in production can be better detected.

The invention discloses an HPLC (High Performance Liquid Chromatography) detection method for ipragmine hydrochloride enantiomers, and belongs to the technical field of pharmaceutical analysis. The HPLC detection method provided by the invention comprises the following steps: dissolving ipragmine hydrochloride to be detected in a diluent to prepare a test solution; detecting the test solution by adopting normal-phase high performance liquid chromatography; the stationary phase of the normal-phase high performance liquid chromatography is silica gel of which the surface is covalently bonded with cellulose-tri (3-chloro-4-methyl phenyl carbamate); a mobile phase of the normal-phase high performance liquid chromatography is a mixed solution of normal hexane, isopropanol, trifluoroacetic acid and diethylamine. According to the detection method established by the invention, the ipragmine hydrochloride main peak and the S-configuration enantiomer impurity peak can be subjected to baseline separation, the separation degree can reach 4.5 or above, the method is high in specificity and sensitivity, and various methodology verification parameters all meet the drug quality control requirements.

The application discloses a method for improving the chromatographic separation degree of impurities A and B in acetylcysteine raw materials, and is characterized by the following steps: controlling the column temperature of a chromatographic column within a specific low temperature range of 15 DEG C plus or minus 2 DEG C, and using the difference between the distribution behaviors of impurities A and B between a stationary phase and a mobile phase at different temperatures to significantly improve the separation degree of the two impurities. When the column temperature is 15 DEG C, the separation degree of impurities A and B can reach 2.06, which is much higher than the pharmacopoeia standard, and the separation degrees under the conditions of 30 DEG C, 35 DEG C and 40 DEG C all fail to meet the standard. The application does not need to replace the chromatographic column, is simple and convenient to operate, low in cost, can stably meet the accurate quantification requirements of related substances in acetylcysteine raw materials, and has good popularization and application value.

The application discloses a method for separating spirodiphenol by using an analog moving bed chromatography. The method for separating spirodiphenol specifically disclosed comprises the following steps: separating left-handed spirodiphenol and right-handed spirodiphenol from a spirodiphenol racemate by using an analog moving bed chromatography system; and the analog moving bed chromatography system has the following conditions: a stationary phase: silica gel coated with chiral polymer; and a mobile phase: a mixed solution of n-hexane and ethanol. The application can realize complete separation of two single enantiomers, obtain high purity and high recovery rate, and is more suitable for industrial production while being continuously operated. The process has strong stability, constant product quality, high automation degree, higher yield and lower cost than batch chromatography. The method also has the advantages of high yield, low cost, recyclable elution phase, green environmental protection, automatic and continuous production, stable product quality and the like.

This invention discloses a method for determining the content of gallic acid and (+)-catechin in *Hedysarum heterotropoides* medicinal materials. After reflux treatment with 60% methanol solution in a water bath, the *Hedysarum heterotropoides* medicinal materials are subjected to high-performance liquid chromatography (HPLC) with octadecyl-bonded silica gel as the stationary phase and acetonitrile (A)-0.1% phosphoric acid aqueous solution (B) as the mobile phase. The peak area values ​​of gallic acid and (+)-catechin showed good linearity in the injection amounts of 10.00–100.0 μg and 200.0–2000 μg, respectively, with average recoveries of 98.31% and 97.68%, and RSD values ​​of 1.2% and 1.2%, respectively. The method is simple and accurate, and can be used for the determination of gallic acid and (+)-catechin content, providing a theoretical basis for the quality control of *Hedysarum heterotropoides* medicinal materials.

Predict the retention time of the target substance obtained by high-performance liquid chromatography. [Solution] In the retention time prediction method, a parametric function is created using a linear combination of the partition coefficients between the mobile phase and multiple stationary phase models. For multiple standard substances, the partition coefficients between the mobile phase and multiple stationary phase models are calculated by quantum chemical calculations using molecular structure information. Using the retention times and partition coefficients of the multiple standard substances, the parameters of the partition coefficients in the linear combination of the parametric function are determined by linear regression. A specific function is created by substituting the parameters into the parametric function. For the target substance, the partition coefficients between the mobile phase and multiple stationary phase models are calculated by quantum chemical calculations using molecular structure information. Using the specific function, the retention time is calculated from the partition coefficients for the target substance.

The invention belongs to the technical field of analytical chemistry, and particularly relates to a method for detecting N-fluorenylmethoxycarbonyl protected histidine and enantiomers thereof, which comprises the following steps: (1) preparing a system applicability solution; (2) injecting the system applicable solution into a high performance liquid chromatograph, and separating by adopting a chiral chromatographic column which takes amylose-tri (3, 5-dimethyl phenyl carbamate) covalently bonded porous silica gel microspheres as a stationary phase; (3) carrying out gradient elution by adopting a binary mobile phase system containing a mobile phase A and a mobile phase B; (4) obtaining a chromatogram map; and (5) calculating the contents of the N-fluorenylmethoxycarbonyl protected histidine and the enantiomer according to the chromatogram. The invention discloses a method for detecting Fmoc-His (Trt)-OH and an enantiomer thereof by an HPLC (High Performance Liquid Chromatography) method. The method has the advantages of very high sensitivity and separation degree, good repeatability and durability, simplicity in operation and stable and reliable result.

This plasmalogen detection method comprises: a sample preparation step for mixing a Schiff's reagent and a specimen containing a plasmalogen to obtain a sample; a reaction step for reacting the plasmalogen in the sample with the Schiff's reagent; and a contact step for bringing at least some of the sample reacted in the reaction step into contact with a stationary phase containing a silica gel.

The invention relates to the technical field of chemical analysis, and discloses a high performance liquid chromatography method for long-chain fatty acid derivative impurities, which comprises the following steps: dissolving a test sample by using tetrahydrofuran and acetonitrile (2: 1) as a solvent, using octadecylsilane (C18) as a stationary phase, using an acidic aqueous solution as a mobile phase A, using an acidic organic solution as a mobile phase B, and using an ultraviolet detector as a detector. High performance liquid chromatography is adopted for gradient elution of the impurities of the long-chain fatty acid derivative, and detection is carried out according to the method, so that the method can effectively detect impurities difficult to separate in the impurities of the long-chain fatty acid derivative, and can be used as a detection method for related substances of the long-chain fatty acid derivative. Impurities which are not easy to separate by a conventional C18 chromatographic column are separated under the system, the separation degree of the impurities and main components is relatively good, the accuracy is high, the operation is simple, and the method has a good application prospect.

The present invention refers to a method for the purification of a collagen-like protein mixture, comprising or consisting of the following steps: a) providing the collagen-like protein mixture in a solvent to form a collagen-like protein solution, followed by heating of the collagen-like protein solution followed by allowing to cool the collagen-like protein solution and b) purifying the collagen-like protein solution of a) by a reversed-phase chromatography which includes a mobile phase in form of at least one mobile phase solvent and a stationary phase in form of at least one column. Furthermore, the present invention refers to the collagen-like protein obtained by the method according to the invention. In addition, the present invention refers to the use of the collagen-like protein obtained by the method according to the invention for medical applications.