315 results about "Stationary phase" patented technology

The invention discloses a fruit and vegetable exosome extraction method based on ligand fishing. The fruit and vegetable exosome extraction method comprises the following steps: suspending amino-functionalized Fe3O4 (at) SiO2 nanoparticles in a glutaraldehyde solution, and adding activated agglutinin to obtain Fe3O4 (at) SiO2-agglutinin nanoparticles; adding a glutaraldehyde solution into agarose gel to obtain a size exclusion chromatographic column filler; the method comprises the following steps: dispersing Fe3O4 (at) SiO2-lectin nanoparticles in a PBS buffer solution to obtain a uniform suspension, adding the uniform suspension into a size exclusion chromatographic column filler to obtain a uniform slurry, filling the size exclusion chromatographic column with the uniform slurry, and flushing with the buffer solution to form a magnetic bead stationary phase; and loading fruit and vegetable supernatant into a size exclusion chromatographic column, passing through the magnetic bead stationary phase, cleaning residual impurities combined on the exosome-magnetic bead compound by using a buffer solution, and dissociating the exosome from the magnetic beads by using a competitive eluent of the exosome to obtain the high-purity fruit and vegetable exosome. The problem that an existing exosome extraction method is difficult to meet the requirements of high efficiency, simplicity, high purity and high activity at the same time is solved.

The invention provides a method for separating EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) through supercritical fluid chromatography, and relates to the technical field of separation and purification. The invention provides a method for separating EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) by supercritical fluid chromatography, which comprises the following steps: by taking supercritical carbon dioxide and an organic reagent as mobile phases, and by utilizing the specific mobile phase property of supercritical CO2 after being mixed with the organic reagent (saturated aliphatic hydrocarbon) and matching with a normal-phase chromatographic column, separating the EPA from the DHA by supercritical fluid chromatography. A specific stationary phase (one or more of a silica gel surface bonded propylurea stationary phase, a silica gel surface bonded phenyl stationary phase and a silica gel surface bonded imidazolyl stationary phase) is adopted, effective separation and preparation of the EPA and the DHA in the fish oil can be achieved, the EPA and the DHA in the fish oil can be effectively separated within 50 min, the separation speed is high, the separation effect is good, and the method is suitable for industrial production. The purity of the obtained EPA and DHA is 98% or above, the purity of the EPA and the DHA is high, and the EPA and the DHA have good application prospects in the fields of medicine production and food and health care products.

A method for separating and purifying 4MP1 from a mixture containing 4MTP2 and 1-Hexene comprises the following steps: continuously introducing a solution in which 4MTP2, 1-Hexene and 4MP1 with any concentration are dissolved into a simulated moving bed chromatography system as a raw material solution, collecting a high-purity 4MP1 solution from raffinate of the simulated moving bed chromatography system by adopting a metal organic framework material as a stationary phase, and separating the high-purity 4MP1 solution from the raffinate of the simulated moving bed chromatography system. The extraction liquid is a solution containing impurities 4MTP2 and 1-Hexene. The invention provides a brand new method for separating 4MP1 from 4MTP2 and 1-Hexene, and a complete separation scheme is provided for the prior art; in the separation of high-concentration 4MP1, the relative purity of the separated and purified 4MP1 can reach gt; the recovery rate is gt; and in the separation of low-concentration 4MP1, the rectification level is also exceeded, and the method is more suitable for 4MP1 thermosensitive substances.

The invention discloses a stationary phase of a novel zwitterionic monomer containing a multi-charge structure as well as a preparation method and application of the stationary phase, and belongs to the technical field of hydrophilic interaction chromatography (HILIC) stationary phases. According to the method, multi-charge zwitterions are used as hydrophilic monomers, the multi-charge structure of the multi-charge zwitterions endows the monomers with higher hydrophilic performance, and the multi-charge zwitterions are immobilized on the surface of a matrix by utilizing an active free radical polymerization method, so that a series of novel super-hydrophilic HILIC stationary phases are finally prepared. The multi-charge zwitterionic monomer adopted by the invention not only has better hydrophilic performance and higher charge density, but also can efficiently, simply and conveniently realize matrix surface grafting and modification. The developed stationary phase is suitable for HILIC column chromatography separation analysis, and the HILIC chromatographic performance can be greatly improved; the method can also be applied to the fields of biological sample pretreatment, material science, marker clinical diagnosis and the like, and has high economic and social values.

The present invention belongs to the technical field of purification of polypeptide drugs, and specifically relates to a method for purifying tilpotide, comprising: (a) preparing a crude tilpotide solution using an acetonitrile aqueous solution; (b) selecting a specific stationary phase, mobile phase, and elution gradient to purify the crude tilpotide solution for a first time to obtain a first fraction; (c) selecting a specific stationary phase, mobile phase, and elution gradient to purify the first fraction for a second time to obtain a second fraction; (d) performing nanofiltration concentration, salt exchange, filtration sterilization, and lyophilization on the second fraction to obtain the tilpotide product. The product obtained by the purification method of the present invention has a purity of more than 99.5%, and the total recovery rate of purification is more than 80%. The single-needle elution cycle of the present invention is only 35 to 40 minutes, which greatly reduces the use of organic solvents in the purification process of tilpotide, reduces purification costs, and is more environmentally friendly in the long term.

This invention discloses an analytical method for benzothiazole contaminants, comprising the following steps: detection by high performance liquid chromatography (HPLC), with the following chromatographic conditions: a phenylhexyl column as the stationary phase, mobile phase A being water, mobile phase B being acetonitrile, and gradient elution; wherein the benzothiazole contaminants are at least one of 2-aminobenzothiazole, 2-hydroxybenzothiazole, benzothiazole, 2-methylbenzothiazole, 2-mercaptobenzothiazole, 2,5-dimethylbenzothiazole, 2-cyanobenzothiazole, ethyl 2-carboxylate benzothiazole, 2-chlorobenzothiazole, 2-bromobenzothiazole, 2-methylthiobenzothiazole, phenylthiocyanate, N-tert-butyl-2-benzothiazole sulfenamide, 2-(2-hydroxyphenyl)-benzothiazole, N-cyclohexyl-2-benzothiazole sulfinamide, dibenzothiazole disulfide, and N,N-dicyclohexyl-2-benzothiazole sulfonamide.

The invention relates to the technical field of floridoside and an isomer monomer thereof, and discloses a method for preparing high-purity floridoside and an isomer monomer thereof on a large scale, which is used for carrying out large-scale preparation and purification research on the high-purity floridoside and the isomer monomer thereof and preparing the floridoside and the isomer monomer thereof from a porphyra haitanensis crude extract. And the structure and purity of the compound are identified. The method comprises the following steps: firstly, by utilizing a DART-MS technology, through a single factor experiment, optimizing experiment parameters such as extraction times and elution, and obtaining optimal extraction efficiency; and secondly, based on a two-step purification method of a prep-HPLC (Prep-High Performance Liquid Chromatography) technology, enriching the target compound by using a reversed-phase (C18) mode stationary phase in the first step, and separating the pair of isomers by using a normal-phase (NH3) mode stationary phase in the second step. The obtained floridoside and isomer monomers thereof are subjected to structure and purity identification by mass spectrometry (MS) and nuclear magnetism (NMR). Results are as follows: the two compounds are identified as floridoside and isomers thereof through high-resolution mass spectrometry, and the purities of the floridoside and the isomers thereof are up to 99.5% or above.

The invention provides a micro-plastic filling permeation immersion type interface coupling reaction experiment device which comprises a rectangular frame, a reaction column cylinder, a T-shaped strip and an iron convex block, a piston rod is movably arranged in the reaction column cylinder, a material guide pipe is arranged at the upper end of the right side of the reaction column cylinder, and a micro plug valve is arranged at a liquid inlet in the lower end of the reaction column cylinder. Guide grooves are formed in the left side and the right side of the interior of the rectangular frame, a magnetic block is installed on the upper portion of the interior of the rectangular frame, a U-shaped iron frame is fixed to the upper end of the right side of an iron protruding block, and a positioning rod is installed at the right end of the lower portion of the U-shaped iron frame in a penetrating and sliding mode. And the reaction solution uniformly permeates through the filling layer by utilizing the negative pressure of the driving core, so that the full-domain immersion type full contact between the micro plastic particles and the reaction solution is realized.

The application relates to the technical field of isotope separation, in particular to a SiO2@CeO2 core-shell material with silica supported cerium dioxide, a preparation method thereof and application of the SiO2@CeO2 core-shell material in Ge / Ga separation. 68 Efficient adsorption of Ge and 68 Effective elution of Ga; the SiO2@CeO2 core-shell material is used as a stationary phase, hydrochloric acid is used as a mobile phase, Ge / Ga is efficiently separated based on solid-phase extraction, and therefore the SiO2@CeO2 core-shell material can be used as 68 Ge- 68 Ga generator column packing.

To provide a method capable of separating a monofunctional species, bifunctional species, etc. of a fluorinated ether compound having a polyfluoropolyether chain and a predetermined functional group in good yield and with high separation performance.A separation method for separating a compound represented by the formula (1) and a compound represented by the formula (2) from a mixture containing them by chromatography using a stationary phase and a mobile phase, wherein the mobile phase contains at least one type of specific solvent selected from a hydrofluoroolefin, a hydrochlorofluoroolefin, a chlorofluoroolefin, a cyclic hydrofluoroolefin, a cyclic hydrochlorofluoroolefin, a cyclic chlorofluoroolefin, a cyclic hydrofluorocarbon, a cyclic hydrochlorofluorocarbon, a cyclic chlorofluorocarbon and a perfluoroketone:A-(OX)m—O—Z—(R)n1  Formula (1)(R)n2—Z—(OX)m—O—Z—(R)n3  Formula (2)

The invention discloses radioactive gas separation and purification equipment, a radioactive gas separation and purification method, a radioactive gas separation and purification medium and radioactive gas separation and purification equipment, and relates to the technical field of radioactive gas separation and purification. The radioactive gas separation and purification equipment comprises a first separation column, a second separation column, a carrier gas container, a quantitative structure, a chromatographic column, a detection assembly, a first control valve, a storage container, a temperature controller and a to-be-treated gas container. The carrier gas container is connected with the first control valve, and the to-be-treated gas container, the quantitative structure, the first control valve, the first separation column, the second separation column, the chromatographic column and the storage container are sequentially connected. The first separation column, the second separation column and the chromatographic column are all filled with stationary phases which are all used for purifying target substances in the to-be-treated gas based on different adsorption rates of the stationary phases relative to different substances. Therefore, due to the arrangement of the first separation column, the second separation column and other structures, large-volume ambient air or gaseous effluent can be purified, and subsequent radioactive substance concentration measurement is facilitated.

The invention discloses an immobilized artificial membrane chromatography (IAMC) stationary phase as well as a preparation method and application thereof, and belongs to the technical field of bionic chromatography stationary phases. According to the method, a matrix containing an initiating group is used as an initiator, under the action of a catalyst, a phospholipid monomer is fixed on the surface of the matrix by utilizing an active free radical polymerization method, and the stable IAMC stationary phase is formed. According to the method, the phospholipid can be efficiently, simply and conveniently grafted on the surface of the silica gel matrix, and the prepared stationary phase has good acid-base stability and bionic property. The IAMC stationary phase provided by the invention has a wide application prospect, can greatly improve the application potential of a chromatographic technology in the fields of life science, environmental protection, drug research and development and the like, and has relatively high economic and social values.

A chromatography system is provided. The chromatography system is configured to process a feed fluid containing a plurality of components, wherein at least one component of the plurality of components of the feed fluid is a target component. The chromatography system comprises: a flow path comprising a plurality of fluid control components configured to control a fluid flow; a stationary phase, wherein the stationary phase is at least one membrane adsorber connected to the flow path and the stationary phase is configured to isolate the target component. The flow path is configured such that harvesting of the target component is optimized.

The utility model provides an environment-friendly row type pigment chromatography device which comprises chromatography tubes, a flow divider and an injector, the chromatography tubes are used for loading a stationary phase, the bottoms of the multiple chromatography tubes are communicated with the flow divider so that the multiple chromatography tubes can be connected to the flow divider in parallel, the injector is used for loading chromatography liquid, and the flow divider is communicated with the flow divider so that the multiple chromatography tubes can be connected to the flow divider in parallel. And the output end of the injector is communicated with the flow divider. According to the invention, multiple groups of pigment chromatography experiments can be completed at one time through the multiple parallel chromatography tubes, and multiple samples can be treated at the same time, so that time and resources are saved, and the experiment efficiency is improved; meanwhile, the environment-friendly row type pigment chromatography device has good air tightness, leakage of poisonous gas in the experiment process can be avoided, after the experiment is finished, chromatography liquid can be recycled through an injector and can be reused, waste liquid discharge is reduced, pollution to the environment is reduced, and the purposes of energy conservation and environment protection are achieved.

The invention discloses a method for simultaneously measuring the concentrations of hydantoin and hydantoin acid based on liquid chromatography, which comprises the following steps: respectively weighing hydantoin and hydantoin acid standard substances, dissolving with a solvent, fixing the volume, shaking uniformly and filtering to prepare a standard substance solution; taking a to-be-detected sample, dissolving the to-be-detected sample with a solvent, fixing the volume, uniformly shaking, filtering to prepare a sample solution, taking the standard substance solutions and the sample solution with different concentrations, respectively carrying out automatic sample injection analysis through a high performance liquid chromatography automatic sample injector, and recording a chromatogram map; establishing a linear regression curve by taking the concentration of the standard substance as an abscissa and the peak area in the chromatogram as an ordinate; and substituting the peak area of the sample to be detected into the linear regression curve to obtain the content of hydantoin and hydantoic acid. According to the present invention, by using the hydrophobic difference caused by the molecular structures of the hydantoin and the hydantoic acid, the retention behavior of the hydantoin and the hydantoic acid on the chromatographic column stationary phase can be adjusted by accurately optimizing the chromatographic conditions, particularly the organic phase / water phase ratio in the mobile phase, such that the efficient and rapid baseline separation is achieved;

The application discloses a kind of cell membrane chromatographic column based on biotin ligase directional covalent fixation and its preparation method and application, belong to chromatography science and technology field. By bonding 2-imino biotin to silica gel, then using biotin ligase specific recognition with Avi-Tag cell membrane fragments, and stable covalent bonding occurs with 2-imino biotin silica gel, so that cell membrane is fixed on the surface of silica gel to obtain Avi-Tag bridged cell membrane chromatographic stationary phase, finally using wet column packing, obtain cell membrane chromatographic column based on biotin ligase directional covalent fixation. The cell membrane chromatographic column can prolong the service life of cell membrane chromatographic column, improve the stability of cell membrane chromatographic column during use, and the short peptide tag (Avi-Tag) used can improve the non-specific adsorption and poor specificity of the cell membrane chromatographic column prepared based on protein tag technology to a certain extent, providing technical support for the wide application of cell membrane chromatographic column.

The invention discloses a method for detecting 5 '(E)-VPA phosphoramidite monomer related substances, and belongs to the technical field of liquid chromatography detection. Related substances comprise 5 '-(E)-VP-2'-OMe-A (Bz), 5 '(Z)-VPA, 5'-(E)-VP-2 '-OMe-A-CE-P and 5' (E)-VPA-OX, and chromatographic detection conditions comprise that the column temperature is 23-27 DEG C, a stationary phase of a chromatographic column is octadecyl bonded silica gel, a mobile phase A is an ammonium acetate solution, a mobile phase B is acetonitrile, gradient elution is performed, and the like. According to the method, four related substances in the 5 '(E)-VPA phosphoramidite monomer are effectively separated through specific liquid chromatography conditions, the system adaptability, specificity, linearity and range, detection limit, quantitation limit and accuracy verification of the method all meet acceptable standards of pharmacopoeia, and detection of multiple related substances can be completed within a short time; and a reliable means is provided for detecting and controlling the quality of the 5 '(E)-VPA phosphoramidite monomer.

Disclosed in the present application is a method for mRNA capping. The method comprises connecting mRNA to a stationary phase and carrying out a capping reaction to obtain a capped mRNA. The present application creatively establishes an immobilized mRNA-based capping reaction-separation coupling strategy and provides an efficient, simple, convenient, and low-cost capping method, and the capping operation can be carried out intermittently and can also be continuously carried out on a column. The present application provides a research basis for the development and application of a novel mRNA production process.

The application discloses a bonded imine three-component spherical COF-based stationary phase and a preparation method and application thereof, and the preparation method comprises the following steps: S1, synthesizing an imine three-component spherical COF containing an alkyne group bonding arm; S2, bonding monosubstituted 6-azido-6-deoxy-3,5-dimethoxy anilino carbamoylated beta-cyclodextrin to the three-component spherical COF prepared in the step S1 by means of a click chemistry method to prepare a bonded imine three-component spherical COF-based chiral stationary phase. The bonded imine three-component spherical COF-based stationary phase has the advantages of simple and convenient synthesis process, good stability of the prepared bonded imine three-component spherical COF-based stationary phase and good application prospect in HPLC separation.

The invention discloses a liquid phase detection method of an oxime ester photoinitiator, and relates to the field of chemical analysis and detection. According to the method, a standard solution, a reaction solution to be detected and a mobile phase are prepared, a sample to be detected is subjected to pretreatment such as quenching and extraction, a high performance liquid chromatography gradient elution technology is combined to realize detection, the mobile phase adopts a phosphoric acid aqueous solution with the concentration of 0.05-0.1% and acetonitrile, a gradient elution program optimizes the separation effect, and a chromatographic column adopts a C18 stationary phase. According to the method, the separation degree of the impurities such as diester impurities in the oxime ester photoinitiator synthesis reaction is larger than or equal to 1.5, the detection limit is as low as 0.010 mg / L, the quantification limit is as low as 0.032 mg / L, the reaction process can be effectively monitored, and the method has the advantages of being easy and convenient to operate, low in cost and high in accuracy.

The present disclosure is directed to stationary phase materials for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified silica particle stationary phase materials, which are optionally also methoxy-terminated polyethylene glycol surface modified.

The invention discloses a method for detecting related substances in testosterone enanthate by high performance liquid chromatography. A mobile phase A and a mobile phase B are used as mixed mobile phases for gradient elution; the mobile phase A is water; the mobile phase B is acetonitrile; the volume ratio of the mobile phase A to the mobile phase B is (5%-70%): (30%-95%); a chromatographic column adopted by the high performance liquid chromatography method is Agilent ZORBAX Eclipse XDB-C8, and the detection method is characterized in that the detection method is simple and easy to implement. According to the method, the testosterone enanthate and the related substances thereof are separated and detected by screening a stationary phase chromatographic column and optimizing a mobile phase elution mode and an elution component ratio, and compared with a method in European Pharmacopoeia, after the chromatographic column is subjected to gradient elution, not only is the retention time of an impurity D prolonged, but also the quality of the impurity D is improved. And the separation degree of the impurity F and the impurity G is increased, so that the elution capacity and the separation degree among the impurities are enhanced.

The invention relates to the technical field of pharmaceutical analysis equipment, and discloses pharmaceutical active component quantitative analysis equipment which comprises a chromatographic column, the chromatographic column comprises a hollow column body, a filter screen is fixedly arranged at the lower end in the column body, a stationary phase is arranged above the filter screen, a piston is arranged above the stationary phase, and the piston is connected with a driving part; the piston comprises a first shell facing the stationary phase, a second shell is coaxially arranged on the side, away from the stationary phase, of the first shell in a sliding mode, an elastic supporting piece is arranged between the first shell and the second shell, a plurality of connecting holes are coaxially formed in the bottom of the first shell and the bottom of the second shell, liquid discharging columns are arranged in the connecting holes, and liquid inlets are formed in the tops of the liquid discharging columns. A liquid outlet is formed in the side wall of the bottom of each liquid discharging column, the multiple liquid discharging columns are connected with a driving mechanism, and the second shell is connected with a liquid inlet pipe. According to the invention, the state of a stationary phase bed layer can be actively adjusted, and uniform introduction and dispersion of liquid medicine are realized, so that the chromatographic separation efficiency and the quantitative analysis accuracy are improved.

The invention discloses a method for detecting amino acid components of cuttlebone medicinal materials, decoction pieces, standard decoction or formula granules and application, and relates to the technical field of traditional Chinese medicine identification. The detection method comprises the following steps: hydrolyzing and extracting cuttlebone medicinal materials or decoction pieces thereof, standard decoction and formula granules to obtain a test solution; dissolving the reference substance with a first hydrochloric acid solution with the concentration of 0.05 mol / L-1 mol / L to obtain a reference substance solution; and / or performing hydrolysis extraction on a cuttlebone reference medicinal material to obtain a reference medicinal material solution; determining by adopting a liquid chromatograph; a chromatographic column of the liquid chromatograph takes octadecylsilane chemically bonded silica as a stationary phase, a mixed solution of acetonitrile and a sodium acetate solution with the concentration of 0.05 mol / L-1 mol / L as a mobile phase A and a mixed solution of acetonitrile and water as a mobile phase B for gradient elution; in the mobile phase A, the volume ratio of the acetonitrile to the sodium acetate solution is 6: 94-8: 92; and in the mobile phase B, the volume ratio of acetonitrile to water is (3: 1)-(5: 1). The detection method disclosed by the invention can be used for determining various amino acid components in the cuttlebone, effectively identifying common similar and counterfeit products, and providing a scientific basis for formulating the quality standard of the cuttlebone.

The invention discloses a glucose-fructose efficient separation and high-purity fructose preparation method, and belongs to the technical field of monosaccharide purification, the preparation method adopts functional resin as a stationary phase and water as an eluent, and chromatographic separation is carried out at 55-65 DEG C; the method specifically comprises the following steps: S1, filling a chromatographic column with functional resin, and balancing with 55-65 DEG C water; s2, pumping a mixed sugar solution of glucose and fructose into a chromatographic column; s3, eluting with water at 55-65 DEG C, and respectively collecting a glucose fraction and a fructose fraction; and S4, respectively concentrating, crystallizing and drying the glucose fraction and the fructose fraction to obtain a high-purity fructose product. According to the separation method, the purity of the obtained glucose is 94.4%-95.1%, the recovery rate of the glucose is 97.1%-98.0%, the purity of the obtained fructose is 98.6%-99.2%, and the recovery rate of the obtained fructose is 95.5%-96.8%.

This disclosure relates to methods for performing size exclusion chromatography. Embodiments of this disclosure are characterized by methods for improving the separation of protein analytes in size exclusion chromatography, for example, by using a low concentration of amino acids or derivatives thereof in the mobile phase and using a stationary phase comprising porous particles with surface-modified polyethylene groups capped with hydroxyl groups.

The invention relates to the technical field of chiral stationary phase fillers, in particular to an underived beta-cyclodextrin chiral stationary phase as well as a preparation method and application thereof. Underived beta-cyclodextrin is bonded on the surface of silica gel through urea or thiourea groups respectively, and the structure is shown as a formula I in the specification. The preparation process of the underived beta-cyclodextrin chiral stationary phase filler provided by the invention has the advantages of simple and easily-controlled conditions, no need of complex derivatization and good repeatability. The novel chiral stationary phase is suitable for different mobile phase systems of high performance liquid chromatography, can efficiently separate N-containing chiral compounds such as imidazole antibacterial drugs and triazole pesticides in various structure types, and can meet the requirements of daily analysis, production process and use process quality control and the like of the chiral drugs and the chiral pesticides.

The present invention provides a method for simultaneously determining 5-hydroxymethylfurfural and its oxidation product, including: providing a reaction mixture obtained after 5-hydroxymethylfurfural is oxidized, the reaction mixture comprising 5-hydroxymethylfurfural and its oxidation product; filtering the reaction mixture to obtain a filtrate and a filter residue; preparing the filtrate as a first solution to be tested, mixing the filter residue with an inorganic base so that the insoluble oxidation product in the filter residue undergoes a salt-forming reaction and dissolves, and preparing the filtrate as a second solution to be tested; performing high performance liquid chromatography analysis on the first solution to be tested and the second solution to be tested, respectively, the conditions for high performance liquid chromatography analysis comprising: an organic acid analytical column as the stationary phase, and a column temperature of 25°C to 60°C; an inorganic acid aqueous solution as the mobile phase, and a flow rate of the mobile phase of 0.4 to 1 mL / min; an injection volume of 1 μL to 20 μL; and adopting dual wavelength detection, with a first detection wavelength of 200 nm to 240 nm and a second detection wavelength of 250 nm to 300 nm. The method provided by the present invention has good separation, high accuracy, and high precision.

The invention relates to a preparation method of a silica ball surface coated polymer and bonded functional stationary phase chromatography filler. The preparation method comprises the following steps: S1, silica gel etherification; s2, coating a polymer on the surface of the vinylated silica gel; and S3, bonding octadecyl or aminopropyl, and performing functional modification on the silicon-based material of which the surface is coated with the polymer to obtain the required silica gel matrix chromatographic filler. According to the preparation method of the silica ball surface coated polymer and bonded functional stationary phase chromatographic filler, the pH range used by silica gel matrix chromatographic filler is expanded, and meanwhile, the polymer on the surface effectively covers silicon hydroxyl on the silica gel surface, so that non-specific adsorption of strong-polarity and alkaline substances is avoided, and the service life of the silica gel matrix chromatographic filler is prolonged. And the separation efficiency of the filler can also be obviously improved.

The application discloses a separation and detection method of methoxy PEG derivatives and methylsulfonyl PEG derivatives, which comprises the following steps: mixing a sample to be detected, ammonia and an ammonium salt catalyst, carrying out a derivatization reaction to obtain a derivative product, and detecting the derivative product by using a liquid chromatography method. The sample to be detected is a methoxy PEG derivative or a methylsulfonyl PEG derivative sample. In the method, the stationary phase of the chromatographic column is octadecylsilane bonded silica gel, the mobile phase A is a 0.01-5% formic acid aqueous solution in terms of volume percentage concentration, the mobile phase B is acetonitrile and methanol in a volume ratio of (1-5):1, a gradient elution program is adopted, and an electrospray detector is used. The method can effectively separate and quantitatively analyze the methoxy PEG derivatives and the methylsulfonyl PEG derivatives, and the method is completely in line with the standards and performs well in the quantitative limit, the linear range, the accuracy and the like, thereby providing an effective guarantee for subsequent product quality control or evaluation.