2498 results about "Stationary phase" patented technology

A method of expanding / maintaining undifferentiated hemopoietic stem cells or progenitor cells by obtaining undifferentiated hemopoietic stem cells or progenitor cells; and either seeding the undifferentiated hemopoietic stem cells or progenitor cells into a stationary phase plug-flow bioreactor in which a three-dimensional stromal cell culture has been pre-established on a substrate in the form of a sheet, the substrate including a non-woven fibrous matrix forming a physiologically acceptable three-dimensional network of fibers, thereby expanding / maintaining undifferentiated hemopoietic stem cells or progenitor cells, or culturing the undifferentiated hemopoietic stem cells or progenitor cells in conditioned medium obtained from such a reactor.

The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and / or increased amount of isoprene. The invention also provides for systems that include a non-flammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.

A golf game employing r.f-tag coded golf balls has a playing area with r.f. antennae located underneath to enable the number of strokes taken by a player to be counted. Separate antennae are provided for the tee area and hole, respectively, and the antennae around the hole are smaller to improve resolution. The antennae are connected to a computer which monitors successive moving and stationary phases of a golf ball to count the number of strokes taken by a player.

A method for detecting information on occupied parking space in a traffic network, in particular a town, uses a parking space detection control center. Parking space information relating to a stationary phase of a vehicle and the vehicle location is transmitted to the control center from a navigation system at the vehicle. Information relating to empty parking space is generated by the control center and information relating to empty parking space is transmitted from the control center to a navigation system at the vehicle end.

A multicapillary sample preparation device, especially useful for handling biological samples, comprising a plurality of uniform capillary tubes coated with a stationary phase, and arranged in a monolithic element. The multicapillary device is suitable for attachment to a pipette, micropipette, syringe, or other analytical or sample preparation instrument.

Pressure-driven microfluidic separation devices, such as may be used for performing high performance liquid chromatography, are provided. Multiple separation columns may be defined in a single device and packed with stationary phase material retained by porous frits. One or more splitters may be provided to distribute slurry and / or mobile phase among multiple separation columns. In one embodiment, separation devices are substantially planar and fabricated with multiple device layers. Systems and methods employing slurry for packing separation devices are also provided.

Novel compositions are disclosed for use as a stationary phase in chromatography comprising an inorganic substrate that is modified with at least one silane having the formula R1δ-Qα-(CH2)βSiR2γX3-γ. In a preferred embodiment, the inorganic substrate is silica gel and is modified with at least two silanes. Methods of preparation and use in chromatographic applications are disclosed. The novel compositions provide superior chromatographic performance, reduced silanol activity, enhanced stability, and reproducibility in preparation and performance.

Chromatographic separation devices include multiple batch-processed columns joined by a body structure and adapted to perform parallel analyses. Both slurry-packed and monolithic column embodiments are provided. One or more liquid-permeable frits of various types may be used to retain stationary phase material within columns. A fluidic distribution network may be used to distribute stationary phase material and / or mobile phase solvents to multiple columns. Separation devices, including microfluidic embodiments, may be fabricated with various materials including polymers. Multi-column fabrication and separation methods are provided.

A method for multi-dimensional chromatography of a polyolefin polymer, comprising introducing a solution of the polyolefin polymer into a liquid flowing through a first liquid chromatography stationary phase or a field flow fractionation device and subsequently flowing the solution through a second liquid chromatography stationary phase, the second liquid chromatography stationary phase comprising graphitic carbon, the polyolefin polymer emerging from the liquid chromatography stationary phase with a retention factor greater than zero.

A stationary vacuum deposition machine for use in a method for processing substrates to make magnetic hard disks includes a series of stations and a transport. The series of stations includes an entrance station for receiving substrates into the machine and a predetermined station. The transport operates in a cycle with each cycle including a transport phase and a stationary phase. The transport causes all the substrates that are in the machine to be moved during the transport phase, and be temporarily held stationary during the stationary phase, such that during each stationary phase a predetermined one of the stations is occupied by one of the substrates while each of a plurality of others of the stations is occupied by a respective one of a plurality of others of the substrates. The machine further includes a plurality of vacuum deposition stations and a scanning beam generator. Each vacuum deposition station operates during each stationary phase such that each station causes a thin film to be deposited on a respective one of the substrates. The scanning beam generator directs a scanning beam at the substrate occupying the predetermined station while the substrate is held stationary to produce a textured pattern.

A separation column and a method of making the separation column are provided. The separation column includes a separation channel and a separation medium in the channel. The separation medium includes a porous matrix, and the porous matrix includes a support and a stationary phase. The support includes a metal organic polymer, such as a photopolymer, and the stationary phase includes a bonded phase. The separation medium can be used to separate a sample of analytes.

Novel compositions are disclosed for use as a stationary phase in chromatography comprising an inorganic substrate that is modified with at least one silane having the formula R1δ-Qα-(CH2)βSiR2γX3-γ. In a preferred embodiment, the inorganic substrate is silica gel and is modified with at least two silanes. Methods of preparation and use in chromatographic applications are disclosed. The novel compositions provide superior chromatographic performance, reduced silanol activity, enhanced stability, and reproducibility in preparation and performance.

The present invention is directed to enantioselective polyelectrolyte complex films. Further, said films may be free or isolated membranes, or coatings on substrates such a porous substrates, capillary tubes, chromatographic packing material, and monolithic stationary phases and used to separate chiral compounds. The present invention is also directed to a method for forming such enantioselective polyelectrolyte complex films.

Devices comprising a mechanism for selectively diverting a portion of a mobile phase flowing through a mobile-phase transporting conduit to a fluid-transporting conduit comprising a stationary phase for separating sample components are disclosed, as well as systems and methods for using the same.

A novel process for the selective elimination of fatty acid compounds containing carbon-carbon double bonds in trans configuration from a substrate containing cis- and trans-isomers of said fatty acid compounds, by selective adsorption by a microporous zeolite material is disclosed. The pore size and shape of usable zeolite materials enable differentiation between cis- and trans-isomers of unsaturated fatty acid chains. The zeolite materials used have a selectivity ratio alphatrans / cis higher than 1.00; this ratio is defined based on the elution properties of cis and trans double bond containing fatty acid methylesters dissolved in n-hexane during a column chromatography experiment with the zeolite material as the stationary phase and n-hexane as the mobile phase. Besides selective adsorption of trans-unsaturated fatty acid compounds, simultaneous or subsequent total or partial hydrogenation of the double bonds in said compounds can be carried out while using the same or similar zeolite material, containing finely dispersed catalytic active metals. The majority of these catalytic active sites must be inside the pores.

A method separating tocotrienols from tocopherols and the isomers thereof on a large-scale or commercially feasible basis. Separation is accomplished using reverse phase partition liquid chromatography. The chromatography column is prepared using a stationary phase comprising a hydrophobic or aromatic reverse phase chromatography media. The mobile phase consists of a solvent which is capable of solubilizing the crude stock, tocotrienols and tocopherols. The chromatography column containing the stationary and mobile phases is loaded with a crude feed stock and the tocotrienols, tocopherols and / or isomers thereof are eluted at a predetermined linear velocity.

Embodiments of the present invention provide a method of chromatographic delipidation comprising separating a lipid-containing sample on a superficially porous stationary phase at greater than about 70° C., at least about 80° C., having at least one mobile phase comprising an ion-pairing agent in water, an ion-pairing agent in an organic modifier, an acid in an organic modifier, and an alcohol. The invention provides minimal protein losses and high run-to-run reproducibility. The on-column delipidation method aventageously utilize reversed phase liquid chromatography.

The invention discloses a rapid prototyping shape memory high polymer material and a preparation method and application thereof. The high polymer material comprises a physical cross-linking hard-segment structure which can be rapidly cooled and formed and a high polymer soft-segment structure which can be fixed and temporarily deformed, wherein the hard-segment structure is formed by combining diisocyanate and a crystallization type chain extender; the soft-segment structure is formed by combining polyester type or polyether type polyhydric alcohol with the crystallization type chain extender; and the molar ratio of hydroxyl in the polyhydric alcohol to diisocyanate group in the diisocyanate is 1:2-1:5. The material is functional, and the printed 3D product has the capability of self-deforming and restoring the time dimensionality; the material has good cooling forming performance, and the hard-segment part can rapidly form a physical cross-linking point in the temperature difference range of melt extrusion; the contained stationary phase and restoration phase double components are wide in material selection and low in price, a preparation process is simple, the defects that synthetic raw materials are expensive, the process is complex and the like in the prior art can be overcome, and the material is suitable for large-scale production.

A non-planar microfabricated gas chromatography column comprises a planar substrate having a plurality of through holes, a top lid and a bottom lid bonded to opposite surfaces of the planar substrate, and inlet and outlet ports for injection of a sample gas and elution of separated analytes. A plurality of such planar substrates can be aligned and stacked to provide a longer column length having a small footprint. Furthermore, two or more separate channels can enable multi-channel or multi-dimensional gas chromatography. The through holes preferably have a circular cross section and can be coated with a stationary phase material or packed with a porous packing material. Importantly, uniform stationary phase coatings can be obtained and band broadening can be minimized with the circular channels. A heating or cooling element can be disposed on at least one of the lids to enable temperature programming of the column.

A reverse phase column chromatographic process of purification of 6-acetyl-4,1′,6′trichlorogalactose and 4,1′,6′trichlorogalactose is described which uses silanized silica as stationary phase and water or predominatly aqeous mixture derived from water and small proportion of organic solvents as a mobile phase.

There are provided ligand compositions and stationary phases comprising a polyhedral oligomeric silsequioxane moiety. Also provided are chromatographic devices comprising the stationary phases, and methods of making and using the ligands, stationary phases and chromatographic devices of the invention.

Nanocolumn chromatography devices useful, e.g., in CEC and nanoLC are disclosed. An exemplary chromatography device of the invention includes a nanocolumn, e.g., a capillary, packed with a particulate stationary phase material and a solid support. The solid support, or in situ frit, is adjacent to and integral with the stationary phase material. An in situ frit of the invention may be a mixture of the stationary phase material and a polymeric network of cross-linked poly(diorganosiloxane), e.g., poly(dimethylsiloxane), which may optionally be sintered. The invention also provides methods of making and using such devices.

An optical data-storage hard disk drive that uses stationary Phase-Change Microhead Array Chips in place of conventional flying-heads, rotary voice-coil actuators, or other similar types of servo-tracking mechanisms to simultaneously record and / or reproduce data to and / or from a multitude of data-tracks located across the data-surfaces of a multitude of phase-change based disc media using a multitude of microheads.

The invention provides super macroporous polymer microspheres and a preparation method thereof. The preparation method comprises the following steps of: firstly, preparing an oil-in-water in-water composite emulsion as a template for super macroporous microspheres through a two-step emulsion process; then, solidifying an oil phase by using a solvent removal method to form super macroporous microspheres provided with inner-outer through pore passages; and finally, after molding the microspheres, further crosslinking microsphere skeleton molecules to obtain microspheres with rigid resin structures. The microspheres prepared by the method have a through pore passage structure, the controllable particle size range is 0.1-300 microns, the controllable pore size range is 0.09-90 microns, and the controllable porosity range is 10-90%. Super macroporous structures are beneficial for biological macromolecules to penetrate through and enter the microspheres, the mass transfer by convection in the microspheres can be realized, and the rigid structure can tolerate higher pressure and higher flow velocity. The super macroporous polymer microspheres can be used as stationary phase fillers for chromatographic separation, immobilized carriers of enzymes, cell culture micro-carriers, tissue engineering micro scaffold materials, adsorbing materials and the like.

A method and apparatus for chromatography of a polyolefin polymer by flowing a solution of the polyolefin polymer through liquid flowing through a graphitic carbon liquid chromatography stationary phase. The method can be used to determine the monomer to comonomer ratio of a polyolefin copolymer such as a copolymer of ethylene and 1-octene or a copolymer of propylene and ethylene.

The present invention is generally directed to a microfabricated gas chromatograph column having two patterned substrates, each optionally having a stationary phase material coating, bonded together to provide a continuous flow channel. The flow channel can have a serpentine arrangement or a modified serpentine arrangement comprising alternating series of consecutive turns in one direction where each series has enough turns to move carrier gas and analyte molecules from the center of the column cross section to an outer wall of the channel or from one outer wall of the channel to the opposite outer wall. Different portions of the substrates can be coated with differing thicknesses of stationary phase material and / or with different stationary phase materials. The column can have a circular cross-section or a semi-circular cross-section where the flat portion of the cross-section has grooves. Also disclosed is the related method of making the microfabricated gas chromatograph column.

Monolithic cartridges including a plurality of nominally aligned polymer fibers can be used as stationary phase materials for liquid chromatography separations. Bundles of fibers are packed together so as to form capillary channels between the fibers. Different polymer compositions permit the “chemical tuning” of the separation process. The fibers can be physically or chemically bonded at spaced locations throughout the cartridge or can be packed together under pressure by use of an encasing wrap to form the capillary channels. Use of fibers allows a wide range of liquid flow rates with very low backpressures. Applications in HPLC, cap-LC, prep-scale separations, analytical separations, waste remediation / immobilization, extraction of selected organic molecules / ions from solution, purification of liquid streams (process waste, drinking water, pure solvents), selective extraction of cell matter and bacteria from growth media, and immobilization of cell matter and bacteria are envisioned.

A novel sorbent suitable for use as a stationary phase in a chromatography column, the core of which consists of an organic polymer of synthetic or natural origin. Further, the carrier exhibits a plurality of covalently bonded non-aromatic zwitterionic groups on its surface. Additionally, the invention also relates to a method for purifying a particular biological macromolecule, such as a protein or a nucleic acid, by zwitterionic ion exchange chromatography as well as an ion exchange column suitable for use in the zwitterionic ion exchange chromatography.

The present invention is a disposable apparatus for the rapid, low-volume solid phase extraction of analytes from a variety of sources. The apparatus of the present invention is configured as a pipette tip and contains a loosely confined stationary phase. The mobility of the stationary phase particles enables rapid mixing and equilibration with a sample solution during agitation. The analyte may thereby be extracted in less time with less solvent, removing the need for a separate concentration step.