76 results about "MICRO PIPETTE" patented technology

A multicapillary sample preparation device, especially useful for handling biological samples, comprising a plurality of uniform capillary tubes coated with a stationary phase, and arranged in a monolithic element. The multicapillary device is suitable for attachment to a pipette, micropipette, syringe, or other analytical or sample preparation instrument.

Here we describe an integrated microfluidic chip for delivery of fluids inside or in proximity of individual cells or for measurement of physical quantities. In this device, a cell suspension is pumped through a microchannel in which cells are lined up, pushed on a static microneedle or micropipette, and then collected in a reservoir. The channels are microfabricated. Valves can be embedded in the system to allow fluid flow control thus enhancing the efficiency of the system and reducing malfunctioning due to clogging or any other unexpected issues.

Disclosed is a device and a process for examining cells using the patch-clamp method. A plane arrangement is used of a first number of micro-cuvettes for receiving cells and a plane arrangement of a second number of micro-pipettes which can be positioned in relation to the plane arrangement of micro-cuvettes in such a manner that a multiplicity of cells located in the micro-curvettes can be examined simultaneously.

The invention relates to a method for preparing silicon nanowires, belonging to the nanophase material preparation technical field. The method comprises the following steps that: a silicon chip is washed and shaken by acetone and alcohol and is processed by an acid cleaning liquid and a No.1 standard cleaning solution; a silicon dioxide spheres solution with a weight percentage density between 0.02 and 2.0 percent is added on the silicon chip surface which is washed off in the step one drop by drop by a micro pipette, the processed silicon chip is placed in air to air naturally; the silicon chip with silicon dioxide sphere arrays is annealed for one to three hours at a temperature of between 900 and 1000 DEG C; afterwards, the silicon chip is put into a diluent hydrofluoric acid solution to be eroded for 2 to 20 minutes so that the diameters of the spheres become small; an Ag film with a thickness between 20 and 80nm is deposited on the silicon chip surface by the plating technique or the vacuum evaporating technique; and a sample deposited with the Ag film is dipped into the hydrofluoric acid and hydrogen peroxide etching solution (or ferric nitrate) to be eroded for 4 to 100 minutes. The preparation method can rapidly prepare a large area of silicon nanowire arrays, thereby being suitable for the large-scale industrial production.

The present invention is directed towards an apparatus and method for disinfecting biological samples, including semen and the like, and for removing a biological sample pellet from surrounding media while reducing the risk of recontamination of the pellet with said media. An insert receptacle includes a receiving cylinder having a drain in a floor which allows the fluids to decant slowly down the side of a tube. The insert receptacle further includes an aspiration channel which allows an aspiration device, such as a modified micro-pipette, to be utilized for extracting a sample pellet from the bottom of the tube. Utilizing the novel apparatus, a method for reducing pathogens in a biological sample includes decanting the biological sample through the receiving cylinder and into a tube where it passes through a multi-layer gradient including an enzyme suitable for removal of at least one pathogen from the biological sample. Centrifuging of the insert receptacle, tube, and sample, isolates a sample pellet of the biological sample from the surrounding at least one pathogen. After the sample pellet is isolated it is aspirated via the aspiration device without re-contaminating the sample pellet through contact with the surrounding at least one pathogen.

The invention relates to a method of realizing the screening and the monoclone preparation of hybridoma cell in one step. The method comprises that the semisolid medium of the monoclone of hybridoma cell is firstly prepared, the basic fibroblast growth factor of human is added into the fusion cell, then hydridoma fusion factor and clone factor are added and well-mixed, then the mixed material and the semisolid medium are gently well-mixed and packaged into plates and cultivated in the condition of 35 DEG C and CO2 with concentration of 5 percent; when visible clone colony grows in the plates, the clone is aspirated from the culture medium by a micropipettor and moved onto a cell culture plate for liquid scale-up culture, and one clone is moved in one hole; till the cell grows to half to two-third of the hole bottom, the culture supernatant is aspirated to do positive detection, and the positive hole is chosen to directly scale-up frozen and stored for further carrying out the characteristic identification of antibody or ascites production. The method of realizing the screening and the monoclone preparation of hybridoma cell in one step has the advantages of the simple operation, the short culture period, not requiring feeder cells and easily achieving the screening and the monoclone preparation of hybridoma cell after cell fusion in one step.

The invention discloses a portable loader for a micropipette tip box. The portable loader comprises a box body, and an upper-layer plate, a fan and a lower-layer plate which are arranged in the box body, wherein the top of the box body is provided with an openable and closable tip inlet, the upper-layer plate is fixed below the tip inlet, the lower-layer plate is arranged below the upper-layer plate, and the fan is fixedly arranged above the lower-layer plate on the box body. The portable loader adopts a simple physical principle, and tips are arranged in transverse slotted holes of the lower-layer plate of the loader by setting the wind speed of the fan, so that the loading efficiency of tips used in a laboratory can be greatly improved, and the labor intensity of operators is reduced by convenient and quick loading. The contamination probability of the tips can be reduced by virtue of the loader. The loader has a simple structure, is easy to process, has a small size, is convenient to operate, can save a space for the laboratory, is also suitable for loading of other tips with various sizes, and can be widely popularized and used.

A method of measuring a sound pressure amplitude in a liquid through using an acoustic suspension liquid drop is disclosed. The method comprises the following steps of firstly, adding a continuous phase fluid into a transparent rectangular groove body; using a micropipettor to add small and non-soluble liquid drops in the continuous phase fluid; starting an ultrasonic transducer and adjusting output power of a power amplifier so that the liquid drops suspend in the continuous phase fluid; through a high speed camera arranged on a side orientation of a rectangular groove, shooting a picture 1 of tiny liquid drops; then making the high speed camera move horizontally along a focal length direction and shooting a picture 2 of a front panel of the groove body with scales; carrying out image processing analysis on the picture 1 and the picture 2; digitizing a suspension position of the liquid drops under electric power, increasing output power of a power amplifier and successively acquiring the suspension positions of the tiny liquid drops under different electric power; and finally, through a theoretical-formula inverse computation, acquiring sound pressure amplitudes under different ultrasonic electric power. By using the sound pressure amplitude measurement method, problems generated when a hydrophone is used to measure the sound pressure amplitude are solved, accuracy is good and applicability is high.

A micro pipette sensing device includes a micro pipette and a sensing device. The micro pipette includes a main portion, an operation portion and at least one tube portion. The tube portion defines an operation space therein. The sensing device is located at a proper position in the operation space of the tube portion. The sensing device includes a MEMS flow sensor for sensing a gas movement in the operation space, thereby effectively improving the precision of solution to be taken and therefore improving the precision of the overall measurement and analysis results.

The invention belongs to the technical field of superfine functional material application and in particular discloses a method for producing liquid marbles by using micro-nano lyophobic granules. The method for producing the transparent liquid marbles comprises the following steps: controlling indoor temperature to be 15-30 DEG C and indoor humidity to be 50% or greater; fully laying a layer of silicon dioxide powder to the inner bottom of a lyophobic plastic container, and covering the surface of the container with the powder completely; dropping 10-1000 microliters of a liquid by using a micropipettor onto the silicon dioxide powder layer; and under the conditions that the frequency is 30-100 Hz and the acceleration is 20-30 m/s<2>, and performing vibration treatment on the liquid droplets and the powder for 30-60 seconds, thereby obtaining the liquid marbles. The liquid marbles produced by using the method have the characteristics of being high in stability and high in transparency, are capable of relatively clearly observing phenomena in experiments, and can be used as environmental-friendly micro biochemical experiment containers for experiments.

The invention relates to an automatic positioning method and a device for tips of different micro pipettes in an automatic microinjection system, belonging to the field of automatic microinjection system. The present invention provides an automatic positioning method for different micro pipette tips in an automatic microinjection system in order to solve the problem that the existing needle tip positioning method is difficult to separate the injection needle from the background, so that the needle tip position of the suction needle cannot be accurately positioned. The automatic positioning method comprises the following steps: pre-processing the acquired original image; the pixel value difference being made between the filtered image and the denoised image; determining a maximum connectingarea of a predetermined number; making the circumscribed rectangle of the connected region, and determining the average position of the intersection point of the circumscribed rectangle and the imageboundary as the root position of the needle tip; calculating the distance between all points in the outer contour and the root position, and the included angle between the line segment connected withthe root position and the horizontal axis of the image; making a distance curve to determine the position of the tip of the needle. The invention is suitable for automatic microinjection of organisms.

A multi-actuated micro-pipette controller is actuated in at least three different ways. First, the multi-actuated micro-pipette controller may be employed similar to a striper for denudation of cumulus cells and transfer of oocytes and embryos by depressing an actuator assembly operably coupled to a ballonet. Second, the multi-actuated micro-pipette controller may be actuated similar to a SWEMED™ denudation pipette by pressing directly on the ballonet while it is seated in a primary housing and a secondary housing, without employing an actuator assembly of the primary housing. Third, the multi-actuated micro-pipette controller may be actuated by removing the ballonet and employing it similar to a PASTEUR™ pipette.

The invention discloses an irregular micro-gel preparation method based on a liquid bridge phenomenon. A height-controllable mechanical lifting platform is adopted, and surface materials with different hydrophilcity/hydrophobicity are fixedly arranged at the fixed end and the liftable end of the mechanical platform respectively. A certain volume of hydro-gel precursor solution is weighed by using a micropipette and is dropwise added between the two surface materials, and the hydro-gel precursor drops are stretched or compressed to form a liquid bridge. By adjusting the shapes of contact parts between the two surface materials and the drops, the material characteristics and the spacing between the two surfaces, liquid bridges with different target shapes can be obtained. The hydro-gel precursor drops with different characteristics can be solidified by using different physical and chemical cross-linking methods. The irregular micro-gel preparation method is simple, convenient and rapid, and is not limited by the materials; the shape is controllable; high-throughput and internal gradient liquid bridges can be directly formed by virtue of simple technical improvement; the irregular micro-gel preparation method is quite potential, and provides powerful support for preparation of a tissue engineering base structure unit in the future.

A fused silica micropipette having a complex shape, and method of manufacture, are disclosed. The fused silica micropipette may be used for microinjection applications, such as assisted conception, wherein the micropipette penetrates a cell membrane prior to injection. A high temperature, laser powered microforge capable of working with fused silica to make the complex structures of the micropipette of the present invention is also disclosed.

The invention discloses a rapid loading device for the gun head of a micro pipette, and belongs to a medical experiment auxiliary tool. The rapid loading device consists of a gun head temporary storage box, a gun head storage tube matrix box, a control board, a storage tube matrix channel, a support, a fixed board, a control board handle and a limiting board, wherein a gun head dredging board is arranged at the bottom of the gun head temporary storage box; the gun head dredging board is provided with a plurality of hopper-shaped dredging holes; 3-6 upright posts are uniformly arranged on the inner periphery of a hopper; a plurality of dredging tubes are arranged in the gun head storage tube matrix box; the perforated control board capable of moving horizontally is arranged below the gun head storage tube matrix box; a storage tube matrix channel and the support are arranged in sequence below the control board; holes in the dredging board and the control board correspond to pipelines in the gun head storage tube matrix box and the storage tube matrix channel one above the other. The rapid loading device has the advantages that a large quantity of disordered gun heads can be orderly placed into a gun head box through simple manual operation, so that high speed and high efficiency are achieved. The rapid loading device is mainly applied to sorting of the gun head of the micro pipette in a laboratory.

The invention belongs to the field of nanometer operation, and discloses a method for large-scale assembling and manufacturing of a ZnO-base nanometer device by adopting floating potential dielectrophoresis. The method includes the first step of preparing dispersive mixing solution, the second step of carrying out ultrasonic-assisted dispersion, the third step of adopting floating potential dielectrophoresis to assemble ZnO nanowires, specifically speaking, applying alternating voltage signals needed by an electric field on a source electrode and a back grid electrode of a floating potential microelectrode chip respectively through probes, taking 1-2mL of dispersed ZnO nanowire sample solution through a micropipettor and titrating the solution to a floating potential microelectrode and applying sine AC signals to the microelectrode, wherein the frequency is 1-2MHz, the peak-to-peak value voltage is 8-10 Vp-p, and the floating potential dielectrophoresis duration is 3-8s, and the fourth step of carrying out aftertreatment, specifically speaking, enabling the electrode chip subjected to the floating potential dielectrophoresis to be rinsed in deionized water and to be heated for 30mins at 105 DEG C in a vacuum drying box, and finally obtaining the ZnO-base nanometer device through annealing. According to the method for large-scale assembling and manufacturing of the ZnO-base nanometer device by the adopting floating potential dielectrophoresis, the floating potential dielectrophoresis technology is adopted, and the problems that the ZnO-base nanometer device is low in effective assembling efficiency and can not be assembled in a large scale are solved.

The presently claimed invention applies to a genetic material processing and manipulation method and related product. The claimed invention relates to a method for changing the inherited characteristics of a cell through micro-beam chromosome modification. In one preferred embodiment, improvements to ‘genomic surgery’ are applied to modify source cell genetic material (101). Source cell (106) is stabilized through micro-pipette (102) applied negative pressure. Excised genetic material (101) undergoes a pre-cutting manipulation step (205) to enhance subsequent genetic manipulation. In an additional embodiment genetic material manipulation is aided by a spindle cutting step (303). The presently claimed invention provides a high quality alternate approach to directed genetic recombination without requiring the use of restriction enzymes and is used for chromosomal repair, removal of defects and new organism creation.

The invention relates to a preparation method of silicosis mouse models. The preparation method of the silicosis mouse models is characterized in that after experiment preparation, isoflurane in an anesthesia machine is adopted to anesthetize mice in a model group, 3 seconds after rapid-shallow breaths of the mice become deep and slow, each mouse is taken out and placed at the center of a palm, the tip of a thumb presses against the underjaw of each mouse, a micropipette is adopted to instill a SiO2 micro-sized particle suspension through the nose of each mouse, the SiO2 micro-sized particle suspension is instilled into the nose wings to be absorbed, closed-chest massage is conducted for 2-3 minutes, then the mice are placed into cages, and after an experiment is finished, the mice are sent back to a normal-temperature animal feed room; only normal saline of the same volume is used for conducting nasal inhalation on mice in a contrast group; weight indexes of the mice are obtained before and after the experiment, the mice are killed at the first week, the second week, the fourth week, the eighth week and the sixteenth week respectively, lung tissue is taken to be pathologically andbiochemically compared and identified, and a model preparation effect is evaluated. According to the preparation method of the silicosis mouse models, a model production technology is stable and efficient; the method can be repeated many times and is noninvasive, environmentally friendly and humanistic, and labor and time are saved.

The invention relates to the technical field of experimental instruments, and discloses a micropipettor which comprises a gun body, a slotted hole is formed in the middle of the gun body, a sleeve isintegrally formed below the gun body, the lower portion of the sleeve is movably sleeved with a gun head, and the middle of the gun body is movably sleeved with a piston matched with the slotted hole.A bumping post movably sleeves the middle part of the top of the gun body and the piston; and a pressure spring movably sleeves the outer surface of the piston and is matched with the slotted hole. According to the micropipettor, the pipetting amount is controlled through the rotary piston, the downward pressing distance of the compression sleeve is directly converted into the suction amount of the gun head, namely the compression storage capacity of the compression spring is the suction amount of the gun head, and the elastic force of the compression spring can directly act on the extensionsleeve through cooperation of the extension sleeve and the compression sleeve, the extension sleeve moves upwards to drive the piston to move upwards for suction, the process that in the prior art, after the pipetting amount value is adjusted, downward pressing is still needed for energy storage for liquid suction is changed, and the operation steps of workers are simplified.

The invention relates to a preparation method of a pipettor for transferring trace liquid drop losslessly based on pyroelectric effect. The method comprises the following steps: preparing a layer of super-hydrophobic layer with uniform thickness on a pyroelectric material, and preparing a layer of light-absorbing layer with uniform thickness on a lower surface of the pyroelectric material to obtain a composite material; fixing a sheet metal with a hole at a center at a light outlet of an infrared laser pen as a diaphragm, then aligning the light-absorbing layer of the composite material with the sheet metal with the hole, and fixing the composite material on a lower surface of the sheet metal with the hole, thereby obtaining the pipettor. The trace pipettor disclosed by the invention can effectively and precisely transfer the quantitative solution, the transferring process is free from any loss, the transferring precision is high, and an inaccurate detection result is avoided.