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Multicapillary device for sample preparation

a multi-capillary, sample technology, applied in the direction of ion-exchangers, separation processes, instruments, etc., can solve the problems of sample molecules that cannot be physically exchanged or diffused from one capillary to another

Inactive Publication Date: 2007-01-25
BIOEDGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0015] The invention is a high surface area multicapillary sample preparation device especially useful for handling biological samples. The multicapillary device does not require use of a silica type porous substrate. Rather, the device incorporates a plurality of parallel capillary tubes, wherein the cavity of each tube remains open and unobstructed throughout sample processing. The capillary tubes of the device function independently of one another so that sample molecules are incapable of being physically exchanged or diffusing from one capillary to another. The multicapillary device is preferably disposed in a housing that is suitable for attachment to a “pipette” or other sample preparation or analytical instrument, enabling the isolation, purification, concentration and / or fractionation of nucleic acids or biological samples in the micro- and nanoliter range, as well as larger mass loads and volumes. In an embodiment of the invention, the multicapillary device features a monolithic element pierced with multiple uniform capillaries. The monolithic element is typically mounted in the lower end of a pipette tip, syringe needle or tubing, and is operated using a pipette.

Problems solved by technology

The capillary tubes of the device function independently of one another so that sample molecules are incapable of being physically exchanged or diffusing from one capillary to another.

Method used

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  • Multicapillary device for sample preparation
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  • Multicapillary device for sample preparation

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0051] C-1 Stationary Phase

[0052] A 5% solution of trimethylchlorosilane in toluene is pumped at 10 μL / min for six hours through a 1 mm outer diameter×25 mm long multicapillary glass rod pierced with approximately 4400 capillaries of 10 μm diameter at 105° C. The multicapillary rod is rinsed with toluene, acetone and methanol, and dried with a nitrogen stream.

example 2

[0053] C-4 Stationary Phase

[0054] A 10% solution of butyldimethylchlorosilane in toluene is pumped at 40 μL / min for six hours through a 2 mm outer diameter×300 mm long multicapillary glass rod pierced with approximately 4600 capillaries of 25 μm diameter at 105° C. The multicapillary rod is rinsed with toluene, acetone and methanol, and dried with a nitrogen stream.

example 3

[0055] C-8 Stationary Phase

[0056] A 10% solution of octyltrichlorosilane in toluene is pumped at 50 μL / min for six hours through a 2.3 mm outer diameter×25 mm long multicapillary glass rod pierced with approximately 1400 capillaries of 40 μm diameter at 105° C. The multicapillary rod is rinsed with toluene, acetone and methanol, and dried with a nitrogen stream.

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Abstract

A multicapillary sample preparation device, especially useful for handling biological samples, comprising a plurality of uniform capillary tubes coated with a stationary phase, and arranged in a monolithic element. The multicapillary device is suitable for attachment to a pipette, micropipette, syringe, or other analytical or sample preparation instrument.

Description

REFERENCE TO RELATED APPLICATIONS [0001] This application claims benefit of U.S. patent application No. 10 / 955,377 filed Sep. 30, 2004, and U.S. Provisional Patent Application No. 60 / 507,474 filed Sep. 30, 2003.BACKGROUND OF THE INVENTION [0002] 1. Field of the Invention [0003] The present invention relates to a multicapillary sample preparation device especially useful for handling biological samples. In particular, the multicapillary device is suitable for use with a pipette, micropipette, syringe, or other similar analytical instrument. [0004] 2. Background Art [0005] Many biological samples are commonly separated by gel electrophoresis and analyzed by matrix assisted laser desorption / ionization mass spectrometry (MALDI-MS). One disadvantage of these techniques, however, is that analysis is strongly affected by the presence of salts, buffers and low molecular weight organic compounds commonly used in the preparation of biological samples. In order to improve the sensitivity and s...

Claims

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Application Information

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IPC IPC(8): B01D15/08G01N30/56G01N30/60
CPCG01N30/56G01N2030/567G01N30/6078G01N30/6043
Inventor BELOV, YURI P.PANTANO, CARLO G.LVOVA, KSENIA
Owner BIOEDGE
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