176 results about "Biological macromolecule" patented technology

The invention belongs to the technical field of membrane separation, and particularly relates to a low-pressure high-flux polyamide / polysulfone hollow fiber composite nanofiltration membrane and a preparation method thereof. The preparation method of the composite nanofiltration membrane comprises the following steps: dissolving sulfonated polysulfone, poly (1-vinyl-3-butylimidazole) bromide and polyvinylpyrrolidone to prepare a spinning solution, and spinning to obtain a base membrane; carrying out hydrophilic modification on the base membrane by using 1, 3-propane sultone; then, an aqueous phase solution containing poly (gamma-glutamic acid) and triethylene tetramine and an organic phase solution containing trimesoyl chloride and 3, 5-diaminobenzoic acid are subjected to interfacial polymerization in the inner cavity of the modified base membrane to form a polyamide separation layer; and finally, carrying out heat treatment to obtain the composite nanofiltration membrane. Through the synergistic effect of zwitterionic ionization of the base membrane and biomacromolecule construction of the separation layer, the mass transfer resistance of water is remarkably reduced, unification of high flux and high selectivity is achieved, and good structural stability is achieved.

The invention relates to the technical field of ion characterization and preparation, in particular to a high-throughput preparation mass spectrum device and method giving consideration to high-resolution characterization. The device comprises an ion source, an ion transmission cavity, a quadrupole mass screening cavity, an ion dissociation cavity, an ion deflection cavity, an ion deceleration and deposition cavity and a high-resolution mass spectrum characterization cavity which are sequentially arranged, wherein the high-resolution mass spectrum characterization cavity is formed in one side of the ion deflection cavity in the vertical direction; soft ionization is combined with a quadrupole analyzer to screen ions, the ions are dissociated through a dissociation cavity, an ion deflection cavity is used for selecting a high-resolution characterization or high-throughput preparation function, a high-resolution mass spectrum characterization cavity is used for achieving high-resolution characterization of screened and fragmented ions, and an ion deceleration and deposition cavity is used for high-throughput preparation of the ions. According to the method, perfect compatibility of high-throughput preparation and high-resolution mass spectrum characterization is realized through ion deflection design, and the method has a wide application prospect in the fields of efficient preparation of biological macromolecules, synthetic macromolecules and the like and the like.

The invention relates to the technical field of biomacromolecule separation and purification engineering, and discloses a chromatographic separation method for removing endotoxin in a vaccine preparation, which comprises the following steps: preparing an equilibrium buffer solution containing citrate with specific concentration and L-arginine hydrochloride; providing a mixed mode chromatography medium with hydrophobic and ion exchange complex ligands and balancing with a buffer; according to the method, through the synergistic effect of 8.0-12.0 mmol / L of citrate and 150-250 mmol / L of L-arginine hydrochloride, the endotoxin micelle structure is destroyed and is induced to be dissociated into monomers, and meanwhile, L-arginine is used for inhibiting monomer aggregation, so that the monomers are diffused into inner holes of the medium in a small size to be adsorbed, and the endotoxin micelle structure is separated into the medium. Therefore, the problem of separation caused by size overlapping of endotoxin micelles and antigens is solved, and double breakthrough of deep removal of endotoxin and high recovery rate of antigens is realized.

The invention relates to the technical field of building materials, in particular to a bio-based multifunctional sound insulation wallboard and a preparation method thereof. The wallboard comprises a sound absorption layer and a sound insulation layer. The sound absorption layer is cooperatively constructed by an inactivated mycelium three-dimensional porous framework and microalgae biomass, and the sound insulation layer is a high-density complex formed by compounding bio-based raw materials such as wood fiber, straw, recycled paper pulp and shell filler. And the two are combined through a biological-physical mechanical interlocking interface formed by hypha growth, extension and embedding to form a natural acoustic gradient structure from porous low acoustic impedance to compact high acoustic impedance. According to the preparation method, an in-situ growth process of mycelium culture and microalgae coating is embedded into a hot-pressing process, a three-dimensional anchoring network is formed by actively embedding mycelia into the sound insulation layer, the microalgae permeates into pores and is subjected to hot pressing to generate biomacromolecules for bonding and filling, a porous structure is synchronously constructed by a one-step method, and firm interlayer bonding is realized. The wallboard has excellent broadband acoustic performance, stable bonding strength and low-carbon and environment-friendly benefits.

The invention relates to an aptamer specifically combined with beta-lactoglobulin and application of the aptamer, and belongs to the technical field of biological detection. According to the aptamer and the preparation method thereof, a binding domain base, participating in recognition, of the aptamer is predicted through molecular docking firstly, then the binding domain base is subjected to directional mutation, the aptamer with the recognition performance improved is obtained, the affinity of the aptamer to beta-lactoglobulin is 10.6 nM, and the aptamer has no recognition capacity on alpha-lactalbumin, casein, bovine serum albumin, immune globulin G, lactose and the like and can be used for preparing the aptamer with the recognition performance improved. Therefore, the aptamer disclosed by the invention has good sensitivity and specificity on the beta-lactoglobulin. On the basis, a fluorescence polarization method is directly constructed by utilizing the characteristic that the beta-lactoglobulin is a biomacromolecule, when the beta-lactoglobulin exists, an aptamer marked by a fluorophore FAM recognizes the beta-lactoglobulin to form an aptamer beta-lactoglobulin compound, and the fluorescence polarization value is relatively large, so that the beta-lactoglobulin compound can be used for identifying the beta-lactoglobulin. Therefore, the specific detection of the low-concentration beta-lactoglobulin is realized.

The invention relates to a circulating tumor cell enrichment, separation and dyeing integrated system and application thereof.The circulating tumor cell enrichment, separation and dyeing integrated system is composed of a bottom plate module, a platform module, a multi-pipette module, a power module and a shell module; the platform module comprises a sample tube rotating and uniformly mixing module, a magnetic adsorption module, a reagent module, a waste liquid pool module and a gun head module, and the sample tube rotating and uniformly mixing module, the magnetic adsorption module and the reagent module are fixedly mounted on a bottom plate I in sequence. The CTC separation and dyeing integrated system is developed on the basis of the immunomagnetic bead technology, enrichment separation and immunofluorescent dyeing of CTC can be automatically completed by running a program, manual operation errors are effectively avoided through a programmed process, and the CTC separation and dyeing integrated system is particularly suitable for clinical CTC detection. The system is also suitable for other enrichment and separation applications based on an immunomagnetic bead technology, including but not limited to enrichment and separation of biological macromolecules such as cells, exosomes, proteins and the like.

Provided is an antibody-drug conjugate. Specifically, the conjugate couples a cytotoxic substance and a biological macromolecule by means of a novel linker. The prepared antibody-drug conjugate has an excellent activity of inhibiting the growth of tumor cells.

The invention discloses a zinc ion battery electrolyte containing phycocyanin as well as a preparation method and application of the zinc ion battery electrolyte. The zinc ion battery electrolyte is prepared from soluble zinc salt, phycocyanin and water, wherein the concentration of the phycocyanin is 0.1 mmol / L to 0.5 mmol / L; the concentration of the soluble zinc salt is 1 mol / L to 2 mol / L. In the zinc ion battery electrolyte disclosed by the invention, phycocyanin is a natural biomacromolecule which is natural, non-toxic, low in cost and environment-friendly, has various structures and is rich in various active functional groups, and the problems of high toxicity, single function, poor economic benefit and high cost of an organic additive in the prior art are solved. Besides, the zinc ion battery electrolyte can inhibit dendritic crystal growth, a stable interface layer is formed on the surface of a zinc negative electrode, side reactions are reduced, and the cycling stability and coulombic efficiency of the battery are improved.

The invention discloses a method for preparing a biomacromolecular drug delivery carrier based on a simple and efficient interface cross-linking system. An amphiphilic polymer monomer containing an epoxy group is prepared through RAFT polymerization so that the amphiphilic polymer monomer can exist on an oil-water interface, meanwhile, a three-arm cross-linking agent which has environmental sensitivity and is terminated by an amino group is prepared, and the amino group on the cross-linking agent and the epoxy group on the amphiphilic monomer are subjected to a cross-linking reaction on the oil-water interface; a drug delivery vehicle is formed. The carrier can entrap biomacromolecules and accurately target tumors through a high-permeability long-retention effect, and glutathione rich in a tumor microenvironment can break disulfide bonds in the carrier and release drugs entrapped in the carrier, so that the aim of treating the tumors is fulfilled. Therefore, the invention provides a drug delivery carrier which is simple, convenient, efficient in entrapment of biomacromolecules and high in stability, and the prepared carrier can be efficiently taken by tumor cells so as to regulate and control apoptosis of the tumor cells and is high in safety.

The application discloses a kind of water-based silicon-based antibacterial antiviral material and preparation method thereof.The material is formed by coating and low-temperature solidification from water-based ceramic precursor slurry, and the slurry includes modified silica sol, functional film-forming silane, self-assembled antiviral precursor solution and other components.By partially grafting amino-rich bioactive macromolecules with epoxy-functional silane, a self-assembled antiviral precursor with core-shell structure is constructed, effectively shielding the positive charge of the biological macromolecule, solving the flocculation problem when mixed with anionic silica sol, and realizing single-component water-based storage.Meanwhile, by utilizing the low surface energy characteristics of long-chain alkyl silane, the antiviral component is driven to migrate to the coating surface during the solidification process, forming a gradient structure with surface enrichment and dense bottom layer.The material does not require high-temperature sintering and can be solidified at low temperature using amino self-catalysis, with excellent storage stability, long-lasting broad-spectrum antiviral performance and good wear resistance.

The invention discloses a device for continuously preparing biological macromolecular micro-nano particles by liquid nitrogen. The device comprises a supporting platform, a freezing flat plate positioned on the supporting platform, a movable spraying frame, a movable scraping plate and a control panel, a liquid nitrogen containing cavity is formed in the freezing flat plate, and the containing cavity is filled with liquid nitrogen; a spraying device is arranged on the movable spraying frame and is used for spraying materials on the surface of the freezing flat plate; the movable spraying frame and the movable scraping plate are arranged at the two opposite ends of the freezing flat plate respectively and electrically connected with the control panel, and the control panel can control the movable spraying frame and the movable scraping plate to move oppositely or reversely in the extending direction of the freezing flat plate; the method has the advantages that continuous production can be achieved, the biological activity of the medicine is reserved, and energy consumption is reduced.

The present application relates to the preparation field of organic polymer monolithic column, in particular to a kind of double-continuous supermacroporous cellulose / polystyrene composite monolithic column and its preparation method and application.Firstly, O / W Pickering emulsion is prepared using modified cellulose nanocrystal (CNC), and Janus CNC-PS with single side grafted polystyrene (PS) polymer brush is prepared by electron activation regeneration-atom transfer radical polymerization (AGET-ATRP) reaction system, and double-continuous phase emulsion gel (Bijel) is prepared by one-step stirring method using Janus CNC-PS with different HLB values as stabilizer.Then, double-continuous supermacroporous cellulose / polystyrene composite monolithic column is obtained by in-situ active polymerization through AGET-ATRP reaction using Janus CNC-PS as macromolecular initiator and Bijel as emulsion template.The obtained monolithic column has uniform skeleton structure, double-continuous skeleton and pore, high mechanical strength, and the unmodified side of CNC covers the outer surface of skeleton, so that the pore surface has hydrophilicity and is easy to modify.The monolithic column effectively overcomes the defects of current polymer monolithic column, such as poor biocompatibility, low mechanical strength and low specific surface area, and has great application potential in the field of enzyme and cell immobilization and biomacromolecule separation and analysis.

The invention relates to an antibody-biomacromolecule conjugate. The antibody-biomacromolecule conjugate comprises an antibody targeting module, a complement effect module and a connexon, the antibody targeting module can be specifically combined with a cell membrane surface antigen, and the complement effect module is a key enzyme C1s in a classical complement pathway or a functional structural domain with complement splitting activity of the key enzyme C1s. The linker is used for connecting the antibody targeting module with the complement effect module, and is designed to generate cutting or conformational change on the outer side of a cell membrane or in an extracellular microenvironment, so that the complement effect module is converted from a limited state to a functional accessible state; to promote initiation of a classical complement pathway in the neighborhood of the cell membrane located by the antibody targeting module. The antibody-biomacromolecule conjugate provided by the invention can be used for engineering regulation and control of complement-related reactions.

The invention discloses a reversible dynamic aperture solid nanopore array based on a temperature-sensitive phase change layer and a preparation method and application of the reversible dynamic aperture solid nanopore array, and belongs to the technical field of single molecule detection and nanostructure regulation, the array introduces the temperature-sensitive phase change layer into the inner wall of a solid nanopore, and when the local temperature crosses the phase transition point of the temperature-sensitive phase change layer, reversible volume change is generated; therefore, the effective aperture of the nanopore is continuously adjustable in a sub-nanometer scale range. An independent micro heating unit and an independent temperature sensing unit are integrated below each nanopore in the array, and single-pore-level accurate temperature control adjustment is achieved in a row-column addressing mode. In combination with ionic current monitoring and an external control module, the aperture can be set in real time according to the size requirements of different target molecules, and dynamic aperture regulation and control with a programmable structure, quick response and good repeatability are realized. The array structure provided by the invention is suitable for single molecule level detection of objects such as multiple types of small molecule pollutants, tumor-related biomarkers, biomacromolecules and the like, and has the advantages of high sensitivity, high flux and wide applicability.

The application discloses a kind of biological hybrid micro machine population oral delivery system of imitating Helicobacter pylori survival mechanism, belong to oral stomach medicine delivery technical field.The system is by active power module (flagellum driven chlamydomonas reinhardtii), environmental regulation module (acid urease modified on algal surface) and multifunctional load module (macrophage membrane wrapped drug-loaded nanoparticles) synergistic assembly through polydopamine (PDA) spatial conformation control layer is formed.Active power module utilizes self-movement ability to penetrate mucus barrier and extend intracavity retention time to 12 hours or more;Environmental regulation module produces local alkalization effect by catalyzing urea hydrolysis, provides chemical shield for system in extremely acid environment of pH 1.0-3.0, protects active power module from gastric acid inactivation;Multifunctional load module is accurately delivered to gastric epithelial cells by inflammation targeting effect, such as CRISPR / Cas9 biological macromolecule, and realizes up to 31.8% in-vivo gene editing efficiency.The application provides a non-invasive, modular active delivery platform for the precise treatment of gastric diseases such as gastritis through the synergistic effect of physical penetration, metabolic reprogramming and gene intervention.

This invention provides a crystallization plate suitable for in-situ X-ray diffraction of biomolecules, comprising: a body and multiple crystallization units formed within the body; each crystallization unit includes a through-hole and a frustum-shaped sample slot support within the through-hole; the lower bottom surface of the frustum-shaped sample slot support is connected to the lower bottom surface of the through-hole, and the upper bottom surface of the frustum-shaped sample slot support is located between the upper top surface and the lower bottom surface of the through-hole; the side surface of the frustum-shaped sample slot support and the side surface of the through-hole form a crystallization buffer bath. The crystallization plate of this invention is applicable to in-situ testing of biomolecular crystals, and features simple operation, good compatibility, high efficiency, and low cost. It can greatly facilitate the rapid collection of single-crystal diffraction data or drug screening at synchrotron radiation crystallography beamlines under room temperature in-situ conditions.

The invention relates to the technical field of separation and concentration of biological macromolecules, in particular to a concentration column for biological macromolecular substances, which comprises a chamber filled with a water absorption material layer and a separation membrane arranged at the bottom of the chamber, the water absorbing material can absorb solvent components to generate osmotic pressure difference to drive solvents in a solution and other substances with the particle size smaller than that of the biological macromolecular substances to pass through the separation membrane, and the biological macromolecular substances are intercepted by the separation membrane. The concentration column can realize separation and / or concentration of biological macromolecular substances without depending on external pressure or centrifugal force.

An amphiphilic drug carrier and the use thereof in the ocular delivery of a biomacromolecular drug. The amphiphilic drug carrier is capable of penetrating the ocular mucus barrier, loading a biomacromolecular drug by means of non-covalent interaction, and delivering the biomacromolecular drug for the treatment of fundus diseases.

The application discloses a fibrin-based hemostatic powder and a preparation method and application thereof. The hemostatic powder is prepared from fibrin and a hydrophilic biological macromolecule through in-situ blending, freeze-drying and grinding, and the hydrophilic biological macromolecule is one or more of carboxymethyl chitosan, epsilon-polylysine, gelatin and collagen. The hemostatic powder has a porous and loose microstructure, exhibits excellent powder forming property, rapid liquid absorption capacity (normal saline or blood) and strong hemostatic performance, and has a wide application prospect.

The present application provides a method of producing a “liquid” array of ligand (such as glycan) modified bacteriophage where the ligand modification is encoded genetically within the bacteriophage genome. This method will allow for the determination of the ligand binding profile of biomacromolecules and cells. Furthermore the method allows the elucidation of ligand-protein interactions where ligand binding is co-operative and synergistic.

The invention relates to the technical field of carbohydrate informatics, in particular to a carbohydrate biomacromolecule property prediction method and system based on a graph neural network and chemical fingerprints. Comprising the following steps: modeling a carbohydrate chain sequence into an undirected graph, regarding monosaccharide and glucosidic bonds as nodes in the graph, introducing a virtual node for storing fingerprint features, extracting molecular fingerprint information from the sequence, constructing two different adjacent matrixes Afull and Aori with virtual node connection and without virtual node connection, and constructing two adjacent matrixes Afree and Aori; the dimension of the fingerprint features is reduced to be consistent with the node features, the features are replaced with molecular fingerprint features by positioning the positions of virtual nodes in the graph, the first three layers transmit Aori to enable a model to learn topological structure information of the graph, and the last layer transmits Afull to achieve fusion of the graph structure and the fingerprints. And pooling and splicing node features obtained after convolution of each layer to obtain final sugar chain representation for predicting different properties of sugar chains. According to the method, the graph structure and chemical fingerprint information of the sugar chain can be effectively combined to obtain more meaningful sugar chain representation.

The invention provides a conjugate as well as a preparation method and application thereof. The conjugate has the structure of M-[L] a (I) wherein M is a biomacromolecule linked to L through a nucleophilic functional group contained in M; a is an integer from 1 to 8; l is a compound of formula (Ia) wherein R1 is an electron withdrawing group; and R2 comprises a functional molecule.

The invention belongs to the technical field of biomacromolecular structure analysis, and particularly relates to a compound system and application thereof in small molecular weight protein cryoelectron microscope structure analysis. In particular, the present invention provides a composite system named Trimbdy, which greatly enhances the overall rigidity and stability of the composite. By utilizing the Trimbdy system provided by the invention, the atomic resolution (less than or equal to 3) cryoelectron microscope structures of a plurality of proteins with the molecular weight of less than 50 kDa are successfully analyzed, and the potential of serving as a simple, universal and efficient tool is proved, so that the Trimbdy system has a good practical application value.

This invention provides the application of trifluoromethanesulfonyl magnetic nanospheres in chemiluminescent immunoassay. The trifluoromethanesulfonyl magnetic nanospheres are used for direct covalent coupling with antigens or antibodies. The specific preparation method includes first forming epoxy magnetic nanobead micelles, then adding trifluoromethanesulfonyl chloride as a modifier in batches under conditions of 0-25°C to obtain a crude product of trifluoromethanesulfonyl magnetic nanospheres. Then, the particles are screened by column chromatography and centrifugation to obtain the final product of trifluoromethanesulfonyl magnetic nanospheres. The trifluoromethanesulfonyl magnetic nanospheres have stronger thermal stability than p-toluenesulfonyl magnetic nanobeads, eliminating the need for an activation step. Under the action of ammonium sulfate catalyst, they are directly covalently coupled with amino-containing biomacromolecules, reducing antibody cross-linking and magnetic bead aggregation caused by activators such as EDC or glutaraldehyde.

Provided is a cell-penetrating peptide capable of delivering a variety of biological macromolecules such as proteins, antibodies, nucleic acids, etc. into cells across the membrane. In addition, a fusion protein, conjugate and complex containing the cell-penetrating peptide, and the use of the cell-penetrating peptide are also provided.

The present application relates to the technical field of biological medicine, and more particularly to a multifunctional antibody K1932 binding human CD19, CD3 and Fc gamma R, wherein the multifunctional antibody is composed of a bivalent Fab fragment specifically recognizing CD19 on the surface of B lymphocyte, a bivalent single-chain antibody recognizing CD3 molecule on the surface of T lymphocyte, and a human immunoglobulin Fc domain binding with Fc receptor, wherein the single-chain antibody recognizing CD3 molecule is connected with the C-terminal of Fab light chain through a connecting peptide Linker1, and the heavy chain and the light chain of the CD3 single-chain antibody are connected by a flexible Linker2; the biological macromolecule with the three functional domains is a highly directional immunotherapy drug, which is suitable for injection administration, can guide T cells to attack CD19-positive B lymphocytes, and the Fc domain is combined with Fc gamma R, so that the in-vivo half-life of the biological macromolecule is adjusted and the unnecessary influence on normal cells is reduced.

This invention belongs to the field of in vitro detection technology, specifically relating to a chemiluminescent immunoassay kit and its application in immunoassay. The chemiluminescent immunoassay kit provided by this invention uses a first target protein recognition molecule and a second target protein recognition molecule to capture proteins. Horseradish peroxidase on the second target protein recognition molecule undergoes a polymerization reaction with a chemiluminescent reagent and norepinephrine, forming a product enriched with the chemiluminescent reagent. Under the action of an alkaline pH adjuster and hydrogen peroxide, a chemiluminescent signal is directly generated. This eliminates the need for covalent modification of the protein recognition molecule with the chemiluminescent reagent, reducing the loss of biomolecule activity, improving the utilization rate of the protein recognition molecule, facilitating the control of batch-to-batch variations of the chemiluminescent reagent, and effectively improving detection sensitivity. The results of the examples show that the chemiluminescent immunoassay kit provided by this invention improves the detection sensitivity of pTau217 to approximately 0.25 pg / mL.

This invention discloses a rapid phenotypic drug susceptibility detection method for Mycobacterium tuberculosis infected samples. The method utilizes the incorporation of deuterium into biomolecules synthesized by metabolically active Mycobacterium tuberculosis in a heavy water (D2O) environment, thereby generating C-D characteristic peaks in single-cell Raman spectroscopy. The inhibition of metabolic activity by anti-tuberculosis drugs leads to a decrease in the C-D signal. By calculating the metabolic change rate between drug-treated and untreated samples, the bacterial drug response can be quantified, and susceptibility / resistance determination and MIC value can be established. This invention provides a rapid phenotypic drug susceptibility detection method for Mycobacterium tuberculosis based on single-cell Raman spectroscopy and heavy water labeling technology (Raman-DIP). This method detects the metabolic changes of individual bacteria under the action of anti-tuberculosis drugs to determine drug susceptibility and minimum inhibitory concentration (MIC), enabling a rapid detection process from sample to result.

The invention discloses a multi-stage chromatographic purification combined cryoelectron microscope sample preparation optimization method and system, and belongs to the technical field of cryoelectron microscope sample preparation. A sample purification time chain is constructed through biological information analysis, a time-purity coordinate system is established based on the time chain, a fluctuation curve is generated, the purification correlation degree between the overall trend index and two adjacent days is calculated, and purification conditions and sample preparation parameters are adjusted according to the positive and negative correlation degree. Data driving is used for replacing experience operation, time correlation of the purification process is captured, collaborative optimization of purification and sample preparation is achieved, the sample purity stability and sample preparation efficiency are effectively improved, and the problems that an existing method depends on experience, lacks dynamic adjustment and is poor in collaboration are solved. The method is suitable for efficient purification and optimization of biomacromolecule (such as protein and nucleic acid compounds) cryoelectron microscope sample preparation.