374 results about "Biological macromolecule" patented technology

The invention discloses a Janus cotton fabric with radiation refrigeration and antibacterial functions and a preparation method thereof.The preparation method creatively achieves multifunctional integration through multistage structural design and interface engineering and specifically comprises the steps that 1, a bionic silicon dioxide nano sheath layer grows on the surface of cotton fiber in situ through a controllable sol-gel method; the monodispersity of the nanoparticles is ensured by optimizing the ratio of the TEOS to the silane coupling agent; (2) introducing high-refractive-index nanoparticles to construct a dual-scale photonic crystal structure, and enhancing solar spectrum reflection by using a plasma resonance effect; (3) performing hydrophilic interface modification by adopting a biomacromolecule self-assembly technology to form a super-hydrophilic antibacterial layer; and (4) constructing a super-hydrophobic interface on the single side of the fabric through precise spraying, and finally forming a Janus structure with an asymmetric wetting gradient. The preparation process is mild in condition and high in controllability, and the obtained Janus fabric has excellent antibacterial performance (the antibacterial rate gt; 99.5%) and radiation refrigeration performance (daytime sub-environment temperature drop gt; 8 DEG C).

The application discloses a chromatography material raw material composition, a chromatography material and a preparation method and application thereof, a whole column and a chromatography pile. The chromatography material raw material composition, with the total weight of raw material A and pore former B being 100%, comprises the following components: 20%-70% of raw material A; 30%-80% of pore former B; 0.1 ‰-1 ‰ of catalyst C; and the raw material A comprises a substance containing a polycyclic epoxy group. The chromatography material composition of the application can solve the pain points of the application of the current chromatography microsphere filler on biological macromolecules. The chromatography material has the advantages of uniform internal structure, good preparation reproducibility, high toughness, modifiable functional ligand and high hydrophilicity, and is more suitable for the separation and purification of biological molecules.

The present invention relates to a method for increasing the content and molecular weight of aloe polysaccharides in aloe vera. The method comprises the following steps: firstly removing the blank part of the tip and tail of the aloe vera, retaining the aloe vera leaves, and cultivating the leaves with pure water; peeling and beating the aloe vera leaves to obtain aloe vera gel; placing the obtained aloe vera gel in a light irradiation reaction tank; setting an agitator at the tank mouth of the light irradiation reaction tank; placing a color-adjustable LED lamp above the light irradiation reaction tank, and subjecting the aloe vera gel to alternating light treatments of blue light, red light, and no light. The present invention successfully increases the content and molecular weight of aloe polysaccharides in aloe vera gel by using an external field such as a red and blue alternating light field. In addition, the application of appropriate light regulation can simulate the natural system and produce biological macromolecules (such as polysaccharides) by controlling the polymerization reaction through the external field. Since the high molecular weight and content of aloe polysaccharides are crucial to the biological activity of aloe polysaccharides, the present invention is of great significance to the development of the medicinal value of aloe polysaccharide products.

The invention provides a preparation method of a calcium emulsifying and embedding system with high bioaccessibility, which comprises the following steps: S1) heating and mixing biomacromolecules and an emulsifier in water to obtain a water phase; the biomacromolecules are protein colloids and / or polysaccharide colloids; the method comprises the following steps: heating and mixing a calcium source substance and anhydrous cream to obtain an oil-solid phase; and S2) mixing the oil-solid phase with a water phase, and emulsifying to obtain the calcium emulsifying and embedding system. Compared with the prior art, the preparation method has the advantages that the natural macromolecular protein and / or polysaccharide with high nutritional value is used as a carrier, the calcium source substance is embedded through a water-oil-solid three-phase emulsification technology, and the preparation method is simple, easy to operate, low in cost and environmentally friendly; in addition, a water-oil-solid three-phase homogenized emulsion is created, the problem that the dispersion stability of milk mineral salt in a liquid food matrix is poor is effectively solved, release of free calcium ions in the small intestine environment can be promoted, and the calcium bioaccessibility is remarkably improved.

The invention relates to an anti-inflammatory and repair-promoting hydrogel for a uterine cavity as well as a preparation method and application of the anti-inflammatory and repair-promoting hydrogel, and relates to the technical field of biological medicines. The uterine cavity anti-inflammatory and repair-promoting hydrogel is prepared from the following raw materials: an anti-inflammatory component, a biomacromolecule with active ester and an amino high-molecular polymer. The hydrogel for resisting inflammation and promoting repair of the uterine cavity, which is prepared by the invention, has good biological safety, injectability and adhesiveness with tissues; in intrauterine adhesion prevention treatment, a three-dimensional network structure formed by crosslinking of the biomacromolecules with the active ester and the amino high-molecular polymer plays a physical isolation role, the anti-inflammatory component plays an anti-inflammatory role by inhibiting expression of an inflammation signal channel, and the biomacromolecules with the active ester and the amino high-molecular polymer achieve a synergistic treatment effect.

The invention belongs to the technical field of membrane separation, and particularly relates to a low-pressure high-flux polyamide / polysulfone hollow fiber composite nanofiltration membrane and a preparation method thereof. The preparation method of the composite nanofiltration membrane comprises the following steps: dissolving sulfonated polysulfone, poly (1-vinyl-3-butylimidazole) bromide and polyvinylpyrrolidone to prepare a spinning solution, and spinning to obtain a base membrane; carrying out hydrophilic modification on the base membrane by using 1, 3-propane sultone; then, an aqueous phase solution containing poly (gamma-glutamic acid) and triethylene tetramine and an organic phase solution containing trimesoyl chloride and 3, 5-diaminobenzoic acid are subjected to interfacial polymerization in the inner cavity of the modified base membrane to form a polyamide separation layer; and finally, carrying out heat treatment to obtain the composite nanofiltration membrane. Through the synergistic effect of zwitterionic ionization of the base membrane and biomacromolecule construction of the separation layer, the mass transfer resistance of water is remarkably reduced, unification of high flux and high selectivity is achieved, and good structural stability is achieved.

The invention relates to the technical field of ion characterization and preparation, in particular to a high-throughput preparation mass spectrum device and method giving consideration to high-resolution characterization. The device comprises an ion source, an ion transmission cavity, a quadrupole mass screening cavity, an ion dissociation cavity, an ion deflection cavity, an ion deceleration and deposition cavity and a high-resolution mass spectrum characterization cavity which are sequentially arranged, wherein the high-resolution mass spectrum characterization cavity is formed in one side of the ion deflection cavity in the vertical direction; soft ionization is combined with a quadrupole analyzer to screen ions, the ions are dissociated through a dissociation cavity, an ion deflection cavity is used for selecting a high-resolution characterization or high-throughput preparation function, a high-resolution mass spectrum characterization cavity is used for achieving high-resolution characterization of screened and fragmented ions, and an ion deceleration and deposition cavity is used for high-throughput preparation of the ions. According to the method, perfect compatibility of high-throughput preparation and high-resolution mass spectrum characterization is realized through ion deflection design, and the method has a wide application prospect in the fields of efficient preparation of biological macromolecules, synthetic macromolecules and the like and the like.

The invention provides a bispecific antibody and a purification method thereof. The purification method comprises the following steps: a) loading a sample containing a bispecific antibody to a affinity chromatographic column, and leaching and eluting; the biomacromolecules in the sample are adsorbed on the affinity chromatography column in the leaching stage, the biomacromolecules comprise antibodies, host cell proteins and protein aggregates, and the antibodies comprise bispecific antibodies and homodimers; separating the antibody from the affinity chromatography column in the elution stage, and collecting the eluate in the elution stage; b) loading the eluent to a hydrophobic membrane material, carrying out flow-through, and collecting a flow-through liquid containing the purified antibody; and the flow-through liquid used for flow-through contains 0.73 to 0.77 M of (NH4) 2SO4. The method can solve the problem that homodimers in bispecific antibodies are difficult to efficiently remove in the prior art, and is suitable for the field of antibody purification.

The invention discloses a method for realizing high-efficiency selenium labeling of protein, which comprises the following steps of: in the process of expressing plasmids containing target protein through a prokaryote expression system, realizing high-efficiency replacement of cysteine sulfur atoms in the target protein by selenium atoms by regulating and controlling the ratio of selenium to sulfur elements in a culture medium; on the premise of keeping a good growth state of cells, the selenium labeling efficiency can reach 86%, and the protein expression quantity is stabilized at about 1mg / g (protein / thallus wet weight); structural analysis shows that the secondary structure of the obtained selenium-labeled protein is basically consistent with that of natural protein and is not obviously changed. The method is easy and convenient to operate, high in labeling efficiency, high in protein yield, good in repeatability and suitable for structural biology research such as nuclear magnetic resonance, an effective means is provided for research of the functional mechanism of sulfur atoms in biomacromolecules (including small peptides, polypeptides, peptide fragments, proteins and protein or peptide complexes) with selenium as a probe, and the method has a wide application prospect. Good scientific research application prospects and industrial popularization values are realized.

The invention relates to an ROS response type persulfide biomacromolecule nano prodrug, a nano preparation and a preparation method and application of the ROS response type persulfide biomacromolecule nano prodrug and the nano preparation, and belongs to the technical field of nano preparations. According to the invention, the hydrogen persulfide is protected by utilizing ROS to respond to a small molecule group, so that the defect that the existing hydrogen persulfide is unstable is overcome; meanwhile, biomacromolecules are used as a carrier of the hydrogen persulfide, so that the liver targeting property, the water solubility and the biological safety of the hydrogen persulfide are improved, and multi-site modification and adjustability of the drug loading capacity are realized; in addition, the nano preparation is simple in reaction process, mild in condition and beneficial to clinical transformation.

The invention belongs to the technical field of nano-drugs, and particularly relates to an artificially evolved protein cage nano-drug as well as a preparation method and application thereof. The artificial evolution protein cage nano-drug provided by the invention comprises an artificial evolution protein cage and a nucleic acid biomacromolecular drug, the artificial evolution protein cage is obtained by artificially obtaining a 240 polymer nano-protein cage C27-A with a PDB number of 6NJ8 by an artificial evolution system, and the amino acid sequence of a protein subunit of the artificial evolution protein cage is as shown in SEQ ID NO.1; the artificially evolved protein cage can be used for efficiently packaging nucleic acid type biological macromolecular drugs inside, has the advantages of high protein yield, good stability, good tumor treatment effect, excellent safety and the like, and can solve the technical problem of low drug packaging efficiency of protein cage nano drugs in the prior art.

The invention discloses immunoregulation hydrogel based on ginsenoside Rd, a preparation method and application, and relates to the technical field of biomedical materials and hydrogels. The hydrogel is prepared by taking natural polysaccharides modified by phenylboronic acid groups, derivatives of the natural polysaccharides and methacrylic anhydride modified protein biopolymers as biological matrix materials; ginsenoside Rd is used as a bioactive functional component, and polyvinyl alcohol is used as a cross-linking agent; a first dynamic reversible cross-linked network is formed through chemical cross-linking, and is endowed with injectability, self-healing property and glucose responsiveness; ginsenoside Rd is taken as a main raw material and then subjected to addition polymerization reaction under the irradiation of a photoinitiator and light, a second static covalent cross-linked network is formed, mechanical strength and structural stability are given to the system, in addition, ginsenoside Rd is loaded in the system through boric acid ester bonds, and the slow-release effect is achieved. The problems of stability and bioavailability during local application of the Chinese herbal medicine extract are solved, and healing of chronic and serious wounds can be more effectively promoted.

The invention relates to the technical field of biomacromolecule separation and purification engineering, and discloses a chromatographic separation method for removing endotoxin in a vaccine preparation, which comprises the following steps: preparing an equilibrium buffer solution containing citrate with specific concentration and L-arginine hydrochloride; providing a mixed mode chromatography medium with hydrophobic and ion exchange complex ligands and balancing with a buffer; according to the method, through the synergistic effect of 8.0-12.0 mmol / L of citrate and 150-250 mmol / L of L-arginine hydrochloride, the endotoxin micelle structure is destroyed and is induced to be dissociated into monomers, and meanwhile, L-arginine is used for inhibiting monomer aggregation, so that the monomers are diffused into inner holes of the medium in a small size to be adsorbed, and the endotoxin micelle structure is separated into the medium. Therefore, the problem of separation caused by size overlapping of endotoxin micelles and antigens is solved, and double breakthrough of deep removal of endotoxin and high recovery rate of antigens is realized.

The invention relates to the technical field of building materials, in particular to a bio-based multifunctional sound insulation wallboard and a preparation method thereof. The wallboard comprises a sound absorption layer and a sound insulation layer. The sound absorption layer is cooperatively constructed by an inactivated mycelium three-dimensional porous framework and microalgae biomass, and the sound insulation layer is a high-density complex formed by compounding bio-based raw materials such as wood fiber, straw, recycled paper pulp and shell filler. And the two are combined through a biological-physical mechanical interlocking interface formed by hypha growth, extension and embedding to form a natural acoustic gradient structure from porous low acoustic impedance to compact high acoustic impedance. According to the preparation method, an in-situ growth process of mycelium culture and microalgae coating is embedded into a hot-pressing process, a three-dimensional anchoring network is formed by actively embedding mycelia into the sound insulation layer, the microalgae permeates into pores and is subjected to hot pressing to generate biomacromolecules for bonding and filling, a porous structure is synchronously constructed by a one-step method, and firm interlayer bonding is realized. The wallboard has excellent broadband acoustic performance, stable bonding strength and low-carbon and environment-friendly benefits.

The invention relates to the technical field of protease separation, in particular to a novel earthworm protease separation and purification process. According to the present invention, the ultra-high speed separation, the molecular weight spectrum comparison extraction and the affinity chromatography purification scheme are adopted, such that the different biological macromolecules in the earthworm can be effectively separated, the earthworm protease-containing cell lysis solution can be concentrated, and most of the impurities can be removed so as to obtain the high-purity protease solution, the method is simple in operation and does not depend on complex chemical reactions, contact between protein and chemical reagents can be reduced, meanwhile, damage of high temperature to earthworm protease is avoided through a low-temperature centrifugation scheme, the enzymatic activity of earthworm protease is kept, high-purity and high-activity earthworm protease is obtained through affinity chromatography purification, operation is easy, and the method is suitable for industrial production.

The invention provides nano bubble water, a preparation method, toothpaste and an oral cavity cleaning care solution, belongs to the technical field of oral cavity cleaning, and relates to corresponding formulas of the toothpaste and the oral cavity cleaning care solution, the particle size of bubbles in the nano bubble water is smaller than 100 nm, and the average particle size of the bubbles is 50-80 nm. According to the nano bubble water and the preparation method, the concentration in the nano bubble water is increased, and the stability is improved; the invention also provides a toothpaste and an oral cavity cleaning care solution, the nano bubble water is used for replacing chemicals such as a chemical bacteriostatic agent and a preservative, and the nano bubble water contains a large amount of active oxygen substances such as hydroxyl free radicals (. OH). The active oxygen has extremely strong oxidation capacity, can quickly oxidize cell walls, cell membranes and internal biomacromolecules of bacteria and viruses, destroys normal physiological structures and metabolic functions of the bacteria and viruses, and enables the bacteria and viruses to lose activity and vitality, thereby achieving the purpose of sterilization. The antibacterial preservative has the advantages of being safe and environment-friendly to human bodies and the environment while having antibacterial and preservative effects.

The invention relates to an aptamer specifically combined with beta-lactoglobulin and application of the aptamer, and belongs to the technical field of biological detection. According to the aptamer and the preparation method thereof, a binding domain base, participating in recognition, of the aptamer is predicted through molecular docking firstly, then the binding domain base is subjected to directional mutation, the aptamer with the recognition performance improved is obtained, the affinity of the aptamer to beta-lactoglobulin is 10.6 nM, and the aptamer has no recognition capacity on alpha-lactalbumin, casein, bovine serum albumin, immune globulin G, lactose and the like and can be used for preparing the aptamer with the recognition performance improved. Therefore, the aptamer disclosed by the invention has good sensitivity and specificity on the beta-lactoglobulin. On the basis, a fluorescence polarization method is directly constructed by utilizing the characteristic that the beta-lactoglobulin is a biomacromolecule, when the beta-lactoglobulin exists, an aptamer marked by a fluorophore FAM recognizes the beta-lactoglobulin to form an aptamer beta-lactoglobulin compound, and the fluorescence polarization value is relatively large, so that the beta-lactoglobulin compound can be used for identifying the beta-lactoglobulin. Therefore, the specific detection of the low-concentration beta-lactoglobulin is realized.

Disclosed in the present invention is a monolithic column material. Quaternary ammonium groups are grafted onto the inner surfaces of pores and channels of the monolithic column material. The quaternary ammonium group is present in the form of EP-OCH2CH(OH)CH-N+R1R2R3. The monolithic column prepared in the present invention is rich in hydroxyl, has excellent compatibility with an eluted analyte containing biomacromolecules, has excellent specific adsorption capacity for nucleic acid substances, and is suitable for enrichment and separation of DNA and / or RNA substances.

The present invention relates to an isolated peptide comprising the amino acid sequence of Formula (I): (O)n-K-(O)m-Z; wherein K is lysine optionally modified with a self-immolative moiety Z is tryptophan or is absent; O is GHGX1Y (SEQ ID NO: 1); G is glycine, H is histidine, Y is tyrosine; each X1 is independently selected from alanine, glycine, serine, asparagine, histidine (H), arginine (R), aspartic acid, tyrosine, and proline; n is 0 – 5; m is 0 – 5; n+m is 3, 4, 5, 6, 7 or 8, and optionally, wherein not all of the X1 residues are proline (P). Such peptide is a coacervate forming peptide. The present invention also relates to methods of preparing coacervate compositions comprising the isolated peptide, optionally also comprising one or more biomacromolecules (payloads). The present invention further relates to the coacervate compositions per se, and their uses, e.g. in methods of treatment or diagnosis.

The invention discloses ionic conductive hydrogel, a preparation method, a wearable flexible sensor and application, and belongs to the technical field of hydrogel. The preparation method comprises the following steps: adding chitosan into an acrylic acid solution, dissolving, adding a peroxide initiator, aluminum salt and a polydopamine solution, and polymerizing at 65-75 DEG C to obtain the ionic conductive hydrogel. By controlling the polymerization temperature and the charging sequence, the contradiction between the high-temperature requirement of peroxy compounds and the thermal degradation of biological macromolecules is broken, and the transparency and the sensitivity of the ionic conductive hydrogel are improved. The obtained ionic conductive hydrogel has high transparency (95%) and high sensitivity, has a wide monitoring range when being used as a hydrogel sensor, can accurately detect micro deformation of 1% strain, still keeps high sensitivity under 600% strain, has a sensitivity coefficient of 47, and has a good application prospect. And the high-end application requirements of a new-generation flexible electronic system in the fields of medical monitoring, intelligent man-machine interaction, wearable equipment, intelligent robots and the like are met.

The invention discloses a compound for preventing and treating related diseases caused by aldehyde metabolism disorder and application of the compound. The compound is a compound as shown in a formula (I) or a stereoisomer, a tautomer, a solvate and pharmaceutically acceptable salt of the compound as shown in the formula (I). The compound can be combined with active aldehyde to reduce the generation of an active aldehyde-biomacromolecule adduct and play a role in removing aldehyde, and can be used for treating various system diseases caused by aldehyde metabolism disorder. # imgabs0 #

The invention provides a biosensor based on heterostructure double holes and a preparation method and application thereof. The device comprises a silicon nitride chip fixed between two electrolyte-filled liquid storage tanks, a layer of graphene film is transferred on the silicon nitride chip in advance, one nanopore is etched on a graphene interface, and the other nanopore is etched on a silicon nitride interface. The two side liquid pools are respectively connected with the positive electrode and the negative electrode of the patch clamp amplifier. When a certain potential difference is applied to the two electrodes, the biological macromolecules in the liquid pool can be captured into the nanometer channel. When two ends of a molecule are captured by two different nanopores at the same time, due to different interaction between a silicon nitride-graphene heterogeneous interface and the molecule and the action of electric field force, the molecule can form a dragsaw phenomenon between the two pores, and the time of the molecule passing through the nanopores is prolonged. According to the invention, the rate of chain molecules such as DNA, protein and the like passing through the nanopore can be regulated and controlled, and accurate control of single molecules and even gene sequencing can be realized.

The invention relates to a circulating tumor cell enrichment, separation and dyeing integrated system and application thereof.The circulating tumor cell enrichment, separation and dyeing integrated system is composed of a bottom plate module, a platform module, a multi-pipette module, a power module and a shell module; the platform module comprises a sample tube rotating and uniformly mixing module, a magnetic adsorption module, a reagent module, a waste liquid pool module and a gun head module, and the sample tube rotating and uniformly mixing module, the magnetic adsorption module and the reagent module are fixedly mounted on a bottom plate I in sequence. The CTC separation and dyeing integrated system is developed on the basis of the immunomagnetic bead technology, enrichment separation and immunofluorescent dyeing of CTC can be automatically completed by running a program, manual operation errors are effectively avoided through a programmed process, and the CTC separation and dyeing integrated system is particularly suitable for clinical CTC detection. The system is also suitable for other enrichment and separation applications based on an immunomagnetic bead technology, including but not limited to enrichment and separation of biological macromolecules such as cells, exosomes, proteins and the like.

The invention relates to a self-immobilized hydrogen peroxide imaging tracer agent as well as a preparation method and application thereof, and belongs to the technical field of nuclear medicine. The invention provides a series of self-fixing type hydrogen peroxide imaging tracers based on aryl boric acid ester. The tracers comprise [18F] HYAS, [18F] HYAT and the like. The series of tracers are high in self-fixing capacity and can react with H2O2 to form QM intermediates, so that the QM intermediates and nucleophilic reagents in biomacromolecules form covalent bonds; in addition, the series of tracers have the physicochemical property of being capable of penetrating through the blood brain barrier; meanwhile, experiments show that the series of tracers can distinguish tissues with different H2O2 concentrations in vivo, and in animal brains treated by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine, ingestion increase of the series of tracers can be observed, and the series of tracers have very strong H2O2 responsiveness. In conclusion, the series of tracers have great application prospects in non-invasive imaging of H2O2 in the brain.

Provided is an antibody-drug conjugate. Specifically, the conjugate couples a cytotoxic substance and a biological macromolecule by means of a novel linker. The prepared antibody-drug conjugate has an excellent activity of inhibiting the growth of tumor cells.

The invention discloses a preparation method and application of a myofibrillar protein emulsion based on enzymatic modification. According to the technology, a myofibrillar protein aqueous suspension is subjected to enzymatic deamidation and glycosylation reaction through protein glutaminase and glucan, a protein-polysaccharide compound with good emulsifying performance is constructed, soybean oil serves as an oil phase, and emulsion systems with different oil phase volume fractions are constructed through a controllable homogenizing technology. The obtained emulsion has the characteristics of natural degradability, excellent rheological property, uniform particle size distribution, long-term physical stability and the like. Compared with a traditional chemical modification technology, the technology adopts a whole bio-based raw material combined enzyme method green technology, the problems of organic solvent residues and the like are effectively solved, and the production cost is reduced while the product safety is ensured. The emulsion has remarkable application advantages in the fields of functional food active ingredient delivery, drug controlled release carriers, cosmetic transdermal absorption systems and the like, and a new thought is provided for development of a biomacromolecule-based delivery system.

The utility model provides an integrated cell lysis device which comprises a shell and a temperature control bin cover, a temperature control module, a semiconductor module and a heat dissipation water row are arranged in the shell, a test tube hole is formed in the temperature control bin cover, and the temperature control module is located below the test tube hole and is in close contact with the test tube hole; the outer side of the semiconductor module is also provided with a heat dissipation cavity, and the heat dissipation cavity is communicated with a heat dissipation water row through a pipeline. According to the device, degradation and denaturation of biological macromolecules in cells can be effectively reduced in the quick-freezing process, and the unfreezing process is beneficial to release of cell contents, so that the extraction efficiency is improved. The device is compact in structure, simple and convenient to operate and efficient in lysis, can automatically perform lysis only by putting a sample into a container and setting a program, has a wide application range, can be suitable for various cell types, and can be used in the fields of scientific research experiments, biotechnology, medical diagnosis and the like.

The present invention relates to a method for calculating the molecular weight of biomacromolecule materials based on AI algorithms. After some biopolymer materials are dissolved into aqueous solutions, it is difficult to form single molecular chains. The present invention selects an ionic liquid as the dissolution system. After the macromolecule materials are dissolved, they can well form single molecular chains, and then rheological methods are used to test the rheological data of the biomacromolecules dissolved in the ionic liquid. The present invention selects the Rouse model as the polymer solution model. By measuring a large amount of data, the model is trained with AI algorithms and non-linear fitting is carried out to obtain the key parameter values, so as to calculate the molecular weight value of the measured materials. This calculation method has the characteristics of accuracy and rapidity, and can be well promoted to the computer research field of polymer materials.

Disclosed is a monolithic column material, primary amine groups or secondary amine groups or tertiary amine groups being grafted on the internal surfaces of pores and channels of the monolithic column material. The primary amine groups, secondary amine groups or tertiary amine groups exist in the form of EP-OCH2CH(OH)CH-NR1R2. A monolithic column prepared by the present invention is rich in hydroxyl groups, has excellent compatibility with elution objects containing biological macromolecules, and also has excellent specific adsorption capacity for nucleic acid substances, making it suitable for enrichment and separation of DNA and / or RNA substances.