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Bio-macromolecular hydrogel and preparation method thereof

A technology of biological macromolecules and hydrogels, which can be used in inactive components of polymer compounds, medical science, prostheses, etc. It can solve the problems of poor mechanical strength, reduced biocompatibility, cytotoxicity, etc., and achieve good mechanical properties. , The preparation method is simple, and the biocompatibility is good.

Inactive Publication Date: 2012-09-26
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011]Technical solution: In order to overcome the poor mechanical strength of biomacromolecular hydrogels and the cytotoxicity and biocompatibility caused by common chemical cross-linking agents or initiators remaining in the system Insufficiency of reduced resistance, the present invention is an enzyme-catalyzed and ion-crosslinked interpenetrating network biomacromolecule hydrogel, which has good biocompatibility and high mechanical strength, and can be used for cell and tissue culture , as a scaffold material for tissue repair or tissue engineering, it can be made into a membrane or porous or tubular shape, etc.

Method used

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  • Bio-macromolecular hydrogel and preparation method thereof

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preparation example Construction

[0031] The preparation method of the biomacromolecule hydrogel of the present invention: dissolve the polysaccharide macromolecule in deionized water to obtain a polysaccharide macromolecule aqueous solution, dissolve proteins or polypeptides in the polysaccharide macromolecule aqueous solution, or add additives to obtain a macromolecular mixed solution . Add an enzyme catalyst, mix evenly, and catalyze the cross-linking reaction at 0°C-50°C for 0.5-24 hours to cross-link the protein or polypeptide to obtain a semi-interpenetrating network hydrogel. The semi-interpenetrating network hydrogel is further cross-linked by divalent ions to obtain a high-strength biomacromolecular hydrogel with an interpenetrating network structure. The protein is one or more of collagen, gelatin, silk fibroin, zein, elastin, and fibronectin; the polypeptide or molecule containing amino acid residues is a small molecule peptide containing lysine and glutamine or One or more of oligomers; polysaccha...

Embodiment 1

[0034] Weigh 0.6g of sodium alginate and dissolve it in 20ml of deionized water to obtain a sodium alginate aqueous solution with a mass concentration of 3%. Weigh 3g of gelatin and add it to the above sodium alginate aqueous solution, heat and dissolve in a water bath at 50°C, and let it stand for defoaming. A homogeneously mixed gelatin and sodium alginate macromolecular aqueous solution is obtained.

[0035] Add 0.5ml of transglutaminase solution with a concentration of 100 U / ml, mix quickly and evenly, place in a flat plate or a columnar mold, and react and crosslink at 40°C for 1 hour to obtain a protein semiinteractive protein crosslinked by gelatin through a peptide bond. Penetrating network hydrogel, which contains alginate macromolecules.

[0036] The obtained semi-IPN hydrogel was taken out from the mold and immersed in a calcium chloride solution with a molar concentration of 5 mol / L for 1 hour to obtain a film-like or block-like interpenetrating network biomacromol...

Embodiment 2

[0039] Weigh 0.1g of guar gum and dissolve it in 10ml of deionized water to obtain an aqueous solution of guar gum with a mass concentration of 1%. Weigh 1g of gelatin and add it to the above aqueous solution of sodium alginate, heat and dissolve in a water bath at 50°C, and let it stand for defoaming. Obtain evenly mixed guar gum and gelatin macromolecular aqueous solution.

[0040] Add 0.5ml of microbial transglutaminase solution with a concentration of 50U / ml, mix quickly and uniformly, place in a tubular or columnar mold, and react and crosslink at 40°C for 1 hour.

[0041] The obtained semi-IPN hydrogel was taken out from the mold, soaked in a CaHPO4 solution with a molar concentration of 10 mol / L for 1 hour, and a tubular or block-shaped interpenetrating network biomacromolecule hydrogel was obtained. The obtained hydrogel is repeatedly washed with water to remove residual calcium ions, and then stored in a deionized aqueous solution. Further freeze-dried at -80°C to ob...

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Abstract

The invention discloses a bio-macromolecular hydrogel prepared by enzyme catalysis and ion cross-linking, and a preparation method of the bio-macromolecular hydrogel. The bio-macromolecular hydrogel comprises a protein or polypeptide bio-macromolecular network formed by enzyme-catalyzed cross-linking of protein or polypeptide or amino acid residue-containing molecule, accounting for 1-99 wt% of total dry mass of the hydrogel; and a polysaccharide bio-macromolecular network formed by bivalent ion cross-linking of polysaccharide macromolecule, accounting for 1-99 wt% of total dry mass of the hydrogel. The above two networks are inter-penetrated without chemical bonding. The hydrogel has the advantages of excellent mechanical properties, no use of chemical cross-linking agent, simple and effective preparation method, good bio-compatibility and mechanical strength, and capability of steam sterilization; may be in the forms of wet or dry film, porous sponge, tube and particles; and can be used in cell / tissue culture, and used as tissue repair material, tissue engineered scaffold or drug release carrier.

Description

technical field [0001] The invention relates to an enzyme-catalyzed and ion-crosslinked high-strength biomacromolecular hydrogel and a preparation method thereof, relates to an interpenetrating network biomacromolecular hydrogel and a preparation method thereof, and belongs to the field of biomedical materials. Background technique [0002] Biomacromolecular hydrogel is a kind of three-dimensional cross-linked network mainly composed of natural substances such as protein and polysaccharide molecules, which can swell in water and retain a large amount of water. Because biomacromolecular hydrogel has a rich water environment, good biocompatibility, viscoelasticity similar to biological tissue structure, and can provide good transport channels for the diffusion of nutrients and bioactive substances, its excellent Water absorption, water retention and biomimetic properties make biomacromolecular hydrogels widely used in biomedical materials and tissue engineering, and can be use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/22A61L27/20A61K47/42C08J3/24C08J3/075C08L89/00C08L5/04C08L5/00
Inventor 李新松文采方硕
Owner SOUTHEAST UNIV
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