62 results about "Microbial transglutaminase" patented technology

A preparation method of using no-rinsing minced fillet of transglutaminase comprises the following steps: removing scale, skin and internal organs of material fish, simply washing, obtaining the minced fillet after separation by a flesh separator, adding sucrose, sorbitol, sodium tripolyph-osphate and focal sodium as antifreezers, adding microbial transglutaminase, stirring at low temperature, packing for shaping, after freezing at temperature of minus 35 DEG C, refrigerating in a cold store with temperature minus 18 DEG C, and the frozen minced fillet without rinsing is the same as the rinsedminced fillet by common method in quality.

The present invention provides a method for producing an improved biomaterial comprising treating a collagen biomaterial with a transglutaminase under conditions which permit the formation of cross-links within the collagen. Preferably, the transglutaminase is a tissue transglutaminase, a plasma transglutaminase or a microbial transglutaminase. In a preferred embodiment, the collagen biomaterial further comprises a cell adhesion factor, such as fibronectin. The invention further provides biomaterials obtainable by the methods of the invention, and medical implants and wound dressings comprising the same.

The invention discloses protein-grafted natural polysaccharide, a preparation method and an application thereof, and concretely relates to bio-protein-grafted chitosan oligosaccharide, a preparation method and an application, which belongs to the high-molecular modification field. The bio-protein-grafted chitosan oligosaccharide is obtained by dissolved chitosan oligosaccharide and a bio-protein solution under effect of a catalyst microbial transglutaminase. According to the invention, reaction condition is mild, technology is simple, and the reaction substances are natural macromolecule with characteristics of environmental protection. The prepared water-soluble chitosan oligosaccharide bio-protein copolymer has good film forming ability, moisture-absorption effect, moisture-retention effect and antioxidation effect.

The invention discloses a glutamine transaminase with improved heat stability and application thereof. High-efficiency expression of microbial transglutaminase (MTG) in escherichia coli serves as a modification platform, a label facilitating improvement of heat stability is added at the C end of MTG maturase, preference is given to IGCIILT to obtain a mutant strain with good enzymatic property, and the heat stability is improved by 2.5 times and 3 times. Modified enzyme is suitable for industrial application, the production cost can be reduced, and the production efficiency can be improved.

The invention discloses a rapid hemostatic product for war wounds, as well as a preparation method and application thereof. The rapid hemostatic product comprises a substrate with added microbial transglutaminase (mTG), wherein the amino acid sequence of the transglutaminase is the protein sequence coded by the gene shown in SEQ ID NO.: 3, or a protein sequence shown in SEQ ID NO.: 4. More specifically, the rapid hemostatic product is selected from a hemostatic agent, a hemostatic device, a hemostatic first-aid kit or a common hemostatic product, the substrate is in a fluid or semi-solid form, which can be absorbed by a human body, or in a malleable gel or colloid form, and the rapid hemostatic product contains the microbial transglutaminase and gelatin. The rapid hemostatic product has the advantages of rapid hemostasis, convenience for use, organ adhesion prevention, wound healing promotion, low toxic and side effects, complete absorption and the like; except for hemostasis in a war, the rapid hemostatic product can also be widely applied to rapid hemostasis for wounds in fire control, accidents and other wild environments.

The invention belongs to the field of biological materials and interdiscipline of polymer chemistry and biochemistry and discloses a gelatin/nano silver composite material decorated by microbial transglutaminase and a preparation method and an application thereof. The gelatin/nano silver composite material decorated by microbial transglutaminase is prepared by utilizing a gelatin/nano silver composite material as main material and utilizing the microbial transglutaminase as a modifier, the material has good biocompatibility, non-toxicity and strong antibacterial activity, the mechanical properties and water stability of the material are good, and meanwhile, the material is simple in preparation process, mild in reaction conditions, easy to control and low in production cost, thereby having good application prospect and very extensive usage in biomedical materials and biomedicine field, such as antibacterial and biodegradable skin dressing capable of reducing infection and inflammation and protecting wound, wound healing agent and artificial skin, and the like.

The invention discloses a method for separating and purifying microbial transglutaminase. The method comprisies the following steps: adding 50-200mg/L dispase in streptomycete fermented liquid, subjecting to ethanol depositing, anion exchange chromatography and cation exchange chromatography, de-salting, concentrating, filtering, and freezing to be dry, so as to obtain the microbial transglutaminase, wherein the purity of the microbial transglutaminase is higher than 95% and the specific activity is 20000-50000U/g. The method disclosed by the invention is simple and is capable of obtaining high-purity transglutaminase from microbial fermentation product with the yield of higher than 70%.

Injectable macroporous hydrogels, and methods of producing same, are described. The injectable macroporous hydrogels may be formed by mixing gelatin microgels with an enzyme. In at least some embodiments, the enzyme may be transglutaminase, and more specifically microbial transglutaminase (mTG).

The present invention relates to a method for generating an antibody-payload conjugate by means of a microbial transglutaminase (MTG). The method comprises a step of conjugating a linker comprising or having the peptide structure (shown in N->C direction) Gly-(Aax)_m-B-(Aax)_n via the N-terminal primary amine of the N-terminal glycine (Gly) residue to a glutamine (Gln) residue comprised in the heavy or light chain of an antibody.

The invention relates to a protein-transglutaminase (TG) reinforcement method of fragile wool fabrics. The method comprises the steps of firstly, dipping the fragile wool fabrics into a keratin solution; dipping the fragile wool fabrics into a TG solution; spreading out the dipped fragile wool fabrics, and carrying out reaction on the fragile wool fabrics for 8-4h at the temperature of 50-60 DEG C; finally, washing the fragile wool fabrics for three times by water, spreading out the washed fragile wool fabrics, and drying the wool fabrics at a shade place. The protein-TG reinforcement method has the beneficial effects that under the help of microbial transglutaminase (MTG), keratin is used for reinforcing the fractured polypeptide chains, so that the appearances of wool fabric cultural relics are not influenced, and the method has prominent superiority in the aspect of high temperature aging resistance.

The invention relates to a method for improving the gel coagulation property of milk protein concentrates and an application of the method. According to the method disclosed by the invention, the gel coagulation property of the milk protein concentrates is improved by treating the milk protein concentrates through a microorganism namely transglutaminase. By controlling the time of a crosslinking reaction to be 1-2h, controlling the reaction temperature to be 33-35 DEG C, controlling the pH to be 7.0-7.5 and controlling the addition quantity of enzymes in protein to be 2.0-2.5U/g, the modified milk protein concentrates with good gel coagulation intensity and good water-holding capacity are obtained. Compared with ordinary milk protein concentrates, the milk protein concentrates under the condition have the advantages that the gel coagulation intensity is improved by 150-206%, and the water-holding capacity is improved by 50-90%. The modified milk protein concentrates are applied to the preparation of stirring type yoghourt, and under the premise that the fermentation time of the yoghourt is not obviously changed, the gel coagulation intensity and the water-holding capacity of the stirring type yoghourt can be notably improved.

The invention discloses a microbe transglutaminase modified soya flour-based wood adhesive and a preparation method thereof, and relates to a wood adhesive and a preparation method thereof. The microbe transglutaminase modified soya flour-based wood adhesive solves the problems that the conventional formaldehyde-type adhesives constantly release formaldehyde, a mode of copolymerizing or blending a soy-based adhesive and synthetic resin improves the water resistance but causes existing volatile toxic substances, consumes a great deal of energy and pollutes environment, and isolated soy protein is adopted as a raw material to modify the soy-based adhesive to cause high cost. The adhesive of the invention is prepared from water, defatted soya flour and microbe transglutaminase. The preparation method comprises the following steps: 1, weighing raw materials; 2, after temperature rise of the water, adding the defatted soya flour, and stirring to prepare slurry; and 3, adjusting pH value of the slurry, adding the microbe transglutaminase, performing stirring reaction, adjusting pH value, stirring again, discharging, and cooling to room temperature. The adhesive has low formaldehyde release, low cost, low energy consumption and no environmental pollution in the using process.

The invention discloses a mulberry resource long-acting hypoglycemic medicine composition as well as granules and a preparation method thereof. The mulberry resource long-acting hypoglycemic medicine composition comprises a mulberry resource hypoglycemic activity extract, a pueraria root flavonoid extract, gelatin and microbial transglutaminase; and the preparation method of the mulberry resource long-acting hypoglycemic granules comprises the following steps: preparing the mulberry resource hypoglycemic activity extract, preparing the pueraria root flavonoid extract, preparing the hypoglycemic granules, and the like. The mulberry resource hypoglycemic medicine composition and the granules prepared by using the method disclosed by the invention can be used for controlling the level of postprandial blood sugar in multi-way and long-acting modes.

The invention discloses microbial transglutaminase and rapid bleeding stopping products for traumatic blood loss in daily life. The products adopt mutant microbial transglutaminase as a main active substrate or an effective component. The raw material microbial transglutaminase used in the invention is a mutant enzyme obtained through gene mutation in the circumstance of not changing the functions and the original low toxicity of traditional microbial transglutaminase, wherein compared with wild type enzymes, the mutant enzyme has amino acid mutagenesis at 4 loci. The enzyme has the advantages of excellent heat resistance and good stability at normal temperature. The rapid bleeding stopping products for the traumatic blood loss in daily life, which are prepared through the mutant enzyme, comprise patches, creams (ointments and plasters), pastes and liniments. Proved by tests, the products disclosed by the invention have the effects of rapidly stopping bleeding, easing pain, preventing wound adhesion, blocking bacteria and the like and have the advantages of low toxic or side effects and convenience in use.

The invention discloses a purification method of an MTGase (Microbial Transglutaminase) crude enzyme. The purification method comprises the following steps: dissolving MTGase crude enzyme powder with water to prepare an MTGase crude enzyme solution with the concentration of 1 weight percent to 2 weight percent; preparing a PEG (Polyethylene Glycol)/(NH4)2SO4 double-water-phase solution to form an upper-layer PEG phase and a lower-layer salt phase; uniformly mixing the MTGase crude enzyme solution with the PEG/(NH4)2SO4 double-water-phase solution; standing at 20 DEG C to 40 DEG C for 1h to 2h; enabling MTGase to enter the salt phase in a double-water-phase system and collecting the salt phase in the double-water-phase system; carrying out nanofiltration membrane treatment on the collected salt phase and collecting inter-membrane liquid of the nanofiltration membrane treatment, so as to obtain purified MTGase. The purification method has the advantages that purification is simple and convenient, the purity of the MTGase obtained by purification and separation is relatively high and the recycling rate is high.

Provided herein are conjugated immunoglobulins and methods for generating conjugated immunoglobulins using a microbial transglutaminase.

The invention discloses a method for improving specific activity of microbial transglutaminase and belongs to the field of enzyme engineering. Mutant enzyme with specific activity improved by 40 percent is obtained by using a site-specific mutagenesis technology to insert a loop, coming from the interior of the transglutaminase maturase and playing an importance effect to TGase catalytic activity, or an amino acid sequence, coming from the vicinity of an catalytic activity center, between streptomyces hygroscopicus transglutaminase leading peptide and maturase, and the method is more suitable for industrial production and application.

In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases. In another aspect, the present disclosure provides glutamine-containing substrates (or Q-tag substrates) that are more resistant to proteases/clipping and therefore, more stable, than other Q-tag substrates, and their uses in substrate tags for cross-linking to an amine-donor tag via an isopeptide bond mediated by a microbial transglutaminase.

The invention discloses a rapid hemostatic product for clinical operation wounds, as well as a preparation method and application of the rapid hemostatic product. The rapid hemostatic product comprises a matrix containing a microbial transglutaminase (mT6), wherein the amino acid sequence of the transglutaminase is the protein sequence coded by the gene shown in SEQ ID NO.: 3, or the protein sequence shown in SEQ ID NO.: 4. Specifically, the rapid hemostatic product is selected from a hemostatic, a hemostatic device, a hemostatic first-aid kit or a common hemostatic product; the matrix adopts a fluid or semisolid form, which can be absorbed by a human body, or a plastic gel or colloid form. The rapid hemostatic product contains the microbial transglutaminase and gelatin. The rapid hemostatic product has the advantages of rapid hemostatic property, convenience for use, prevention on organ adhesion, promotion on wound healing, low toxic and side effects, complete absorption and the like. The rapid hemostatic product can be used as a medical hemostatic material or product for clinical operation wounds, can stop bleeding rapidly, prevents organ adhesion, is easy to absorb, has little possibility of spreading diseases, and is low in cost.

The invention relates to the use of an immunologically reactive microbial transglutaminase or its immunologically reactive parts or analogues, which are present in a complex with gliadin or its immunologically reactive parts or analogues, for the diagnosis and/or therapy control of coeliac disease or sprue as well as gluten sensitivity, and a kit for determining the diagnosis and/or therapy control of coeliac disease or sprue as well as of gluten sensitivity, by means of the previously mentioned complex.