Gelatin/nano silver composite material decorated by microbial transglutaminase as well as preparation method and application thereof

A technology of transglutaminase and composite material, applied in the field of gelatin/nano-silver composite material and its preparation, can solve the problems of unsatisfactory mechanical properties of gelatin-based material, affecting the normal growth of tissues and cells, etc. Infect bacteria, improve mechanical and mechanical properties and stability, and prepare simple effects

Inactive Publication Date: 2013-10-23
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the mechanical properties of gelatin-based materials are not ideal, so further cross-linking modification is usually required to improve the mechanical properties of the material
Commonly used chemical modifiers include aldehydes (formaldehyde, glutaraldehyde), epoxy compounds, and carbodiimides, etc., but chemical modifiers are often cytotoxic, causing the modified biomaterials to affect tissue after implantation. and normal cell growth

Method used

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  • Gelatin/nano silver composite material decorated by microbial transglutaminase as well as preparation method and application thereof
  • Gelatin/nano silver composite material decorated by microbial transglutaminase as well as preparation method and application thereof
  • Gelatin/nano silver composite material decorated by microbial transglutaminase as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A gelatin / nano-silver composite material modified by microbial transglutaminase is prepared by the following method:

[0026] (1) Preparation of nano-silver: weigh 0.14g AgNO 3 Add to 200mL deionized water, stir and dissolve at room temperature; weigh 0.19g NaBH 4 Add to 25mL deionized water, sonicate at room temperature for 5 minutes to get NaBH 4 solution; to the above AgNO 3 Add 0.06g of sodium citrate to the solution, and then add NaBH dropwise to the solution at a rate of 2 drops / second under constant temperature magnetic stirring at 25°C. 4 Solution, stop dripping until the solution turns yellowish brown, continue to stir and react for 8 hours; finally, centrifuge the reacted solution, wash the precipitate with ethanol and acetone for 3 times, and dry it in vacuum at 40°C to obtain silver nanoparticles;

[0027] (2) Solution preparation: weigh 6g of gelatin and add it to 100mL deionized water, stir in a water bath at 50°C for more than 2 hours until the gelatin...

Embodiment 2

[0034] A gelatin / nano-silver composite material modified by microbial transglutaminase is prepared by the following method:

[0035] (1) Preparation of nano-silver: weigh 0.1g AgNO 3 Added to 100mL deionized water, stirred and dissolved at room temperature to obtain AgNO 3 solution; weigh 0.15g NaBH 4 Add to 20mL deionized water, sonicate for 3 minutes at room temperature to dissolve NaBH 4 solution; to the above AgNO 3 Add 0.04g of sodium citrate to the solution, and under constant temperature magnetic stirring at 20°C, add NaBH dropwise to the solution at a rate of 3 drops / second 4 Solution, until the solution turns yellowish brown, stop the dropwise addition, and continue to stir and react for 6 hours. Finally, the reacted solution was centrifuged, the precipitate was washed twice with ethanol and acetone, and vacuum-dried at 35° C. to obtain silver nanoparticles;

[0036] (2) Solution preparation: weigh 4g of gelatin and add it to 50mL deionized water, stir in a water...

Embodiment 3

[0039] A gelatin and nano-silver composite material modified by microbial transglutaminase is prepared by the following method:

[0040] (1) Preparation of nano-silver: weigh 0.2g AgNO 3 Add to 300mL deionized water, stir and dissolve at room temperature; weigh 0.3g NaBH 4 Add it into 40mL deionized water, and dissolve it by ultrasonication at room temperature for 8 minutes; 3 Add 0.08g of sodium citrate to the solution, and then add NaBH dropwise to the solution at a rate of 4 drops / second under constant temperature magnetic stirring at 30°C. 4 Solution, until the solution turns yellowish brown, stop the dropwise addition, and continue stirring for 10 hours. Finally, the reacted solution was centrifuged, the precipitate was washed 4 times with ethanol and acetone, and vacuum-dried at 45° C. to obtain silver nanoparticles.

[0041] (2) Solution preparation: weigh 8g of gelatin and add it to 150mL of deionized water, stir in a water bath at 60°C for more than 3 hours until t...

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Abstract

The invention belongs to the field of biological materials and interdiscipline of polymer chemistry and biochemistry and discloses a gelatin/nano silver composite material decorated by microbial transglutaminase and a preparation method and an application thereof. The gelatin/nano silver composite material decorated by microbial transglutaminase is prepared by utilizing a gelatin/nano silver composite material as main material and utilizing the microbial transglutaminase as a modifier, the material has good biocompatibility, non-toxicity and strong antibacterial activity, the mechanical properties and water stability of the material are good, and meanwhile, the material is simple in preparation process, mild in reaction conditions, easy to control and low in production cost, thereby having good application prospect and very extensive usage in biomedical materials and biomedicine field, such as antibacterial and biodegradable skin dressing capable of reducing infection and inflammation and protecting wound, wound healing agent and artificial skin, and the like.

Description

technical field [0001] The invention belongs to the fields of biomedical materials, polymer chemistry and biochemistry, and in particular relates to a gelatin / nano-silver composite material modified by microbial transglutaminase and its preparation method and application. Background technique [0002] As a protein-like natural polymer and biomacromolecular material with abundant natural sources, gelatin has good biocompatibility, no antigenicity under physiological conditions, can be completely absorbed in the body, and is non-toxic to the human body. It has a wide range of applications in skin, wound dressing and healing agents. However, gelatin material is brittle, easily broken, and easily absorbs water and swells; in addition, gelatin is a proteinaceous substance that can provide rich nutrients for bacteria, so it is easy to breed bacteria in a humid environment; and gelatin will occur at higher temperatures. Dissolution, these limit the application of gelatin in biomat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08L89/00A61L15/32A61L15/18A61L15/42A61L27/60A61L27/42A61L27/48A61L27/50
Inventor 熊燕飞王燕梅王海斌潘晨郭雪成韩梅王艺峰
Owner WUHAN UNIV OF TECH
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