119 results about "Artificial skin" patented technology

The invention provides a recombinant humanized elastin with high biological activity as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering and biological materials. 100% of the amino acid sequence of the elastin is derived from human elastin and is prepared by expression of genetically engineered bacteria; the protein has a typical beta-spiral hydrophobic structural domain and a lysine-rich hydrophilic structural domain, has a good bionic structure and functional characteristics, can significantly promote proliferation, adhesion and migration of human skin fibroblasts, also has excellent antioxidant, anti-wrinkle and anti-aging activities, and can be used for preparing a biomimetic protein. The hydrogel can be widely applied to the fields of skin repair dressings, injection implants, artificial skin, biomedical materials, functional skin care products and the like; in addition, the elastin is good in solubility, high in stability, mature in expression system and suitable for large-scale production.

The present invention discloses a waterborne polyurethane and silica gel composite biomimetic material, its preparation method and application, belonging to the technical field of biomimetic skin. The biomimetic material includes a silica gel layer and a polyurethane layer. The material of the silica gel layer includes two-component silica gel, and the material of the polyurethane layer includes double-bond terminated waterborne polyurethane. The preparation method of the biomimetic material is as follows: Spray the double-bond terminated waterborne polyurethane into a mold, heat and dry it to obtain a polyurethane layer; Spray a platinum catalyst on the surface of the polyurethane layer, then mix the A component and B component of the two-component silica gel evenly and pour them into the mold, oscillate and level them, and cure and form to obtain the waterborne polyurethane and silica gel composite biomimetic material; The biomimetic material has the characteristics of high peel strength, good environmental performance, good folding resistance, high wear resistance, good heat resistance, etc., and especially has a baby-like skin-friendly touch, and has broad application prospects in artificial skin, soft robots, wearable devices, prosthetic epidermis, etc.

The invention relates to artificial skin, in particular to self-generating full-layer artificial skin for wound treatment and application of the self-generating full-layer artificial skin. The artificial epidermis has an epidermis-dermis structure similar to skin, and the artificial epidermis structure is composed of an electrostatic spinning nano-film with piezoelectric capacity and has the functions of sterilization, protection and bioelectricity generation of an epidermis layer; the artificial dermal structure is composed of a photo-crosslinking polysaccharide and enzyme crosslinking protein interpenetrating hydrogel skeleton, by adding bioactive components and loading living cells, the effects of releasing active components and cytokines, remodeling wound extracellular matrix and promoting cell proliferation are achieved, and finally healing of acute and chronic wounds is remarkably accelerated. The invention provides a simple, feasible, economical and effective treatment method for sealing a large-area wound, sterilizing the wound and promoting healing of the wound.

The invention discloses a waterborne polyurethane and silica gel composite biomimetic material and a preparation method and application thereof, and belongs to the technical field of biomimetic skin, the biomimetic material comprises a silica gel layer and a polyurethane layer, the silica gel layer is made of two-component silica gel, the polyurethane layer is made of double-bond end-capped waterborne polyurethane, and the two-component silica gel is made of polyurethane. The preparation method of the biomimetic material comprises the following steps: spraying double-bond end-capped waterborne polyurethane into a mold, heating and drying to obtain a polyurethane layer; spraying a platinum catalyst on the surface of the polyurethane layer, then uniformly mixing the component A and the component B of the two-component silica gel, pouring the mixture into a mold, oscillating, paving, curing and forming to obtain the waterborne polyurethane and silica gel composite biomimetic material. The biomimetic material has the characteristics of high peel strength, good environmental protection performance, good folding resistance, high wear resistance, good heat resistance and the like, particularly has a skin-friendly touch feeling like a baby, and has a wide application prospect in the aspects of artificial skin, soft robots, wearable equipment, artificial limb epidermis and the like.

The invention discloses a skin care product anti-aging effect multi-dimensional comprehensive detection system, which comprises an in-vitro cell model experiment module for detecting indexes such as cell activity, proliferation, migration, apoptosis and antioxidant enzyme activity by simulating natural aging and light aging conditions; the content change of skin structure components such as collagen, elastic fibers and laminin is quantitatively analyzed, the DNA damage repair capacity is reflected by measuring the expression quantity of gammaH2AX, epigenetics research is developed, and the methylation state change of a CpG island is monitored; and the tissue engineering and three-dimensional skin model experiment module is used for performing morphological observation, biochemical index detection and skin physiological parameter evaluation under the condition of simulating a real skin environment by utilizing an artificial skin model. The whole process from the in-vitro cell model to the clinical human body test is covered, and the actual efficiency of the skin care product for delaying or improving the skin aging process can be accurately and objectively evaluated through multi-angle and multi-level data integration analysis.

The invention belongs to the field of human health exposure research, and particularly relates to an efficient method for measuring adsorption and release parameters of volatile organic compounds (VOCs) of skin or artificial skin. The method comprises the following steps: designing a skin loader for bearing experimental skin and keeping the experimental skin in a hydration state in an experimental process; a testing system suitable for skin VOCs adsorption and release is developed; establishing a physical model for describing a skin VOCs release process; the skin to be measured is placed in the constant-temperature environment chamber, sufficient VOCs gas is injected to enable the skin to be adsorbed to a balanced state, then pure air is introduced to promote the skin to release VOCs, and meanwhile the gas-phase VOCs concentration in the chamber is measured. And carrying out nonlinear fitting on the concentration data by combining particle swarm optimization and an ant colony algorithm, and calculating diffusion coefficients (Dm) and distribution coefficients (K) of different VOCs in the skin. The testing system is low in cost, easy and convenient to operate, high in calculation method precision, good in expansibility, suitable for testing of other materials and capable of being widely applied to laboratories and engineering application.

The invention provides a preparation method of a composite artificial flap with a bionic structure and function induction activity. The preparation method comprises the following steps: S1, preparing an epidermis induction layer; s2, preparing a dermis functional remodeling layer; s3, preparation of a vascularization integration substrate layer; and S4, three-layer compounding and sterilization. Through an epidermis-basilar membrane-dermis three-layer integrated structure, a micron-nano multistage gradient pore and basilar membrane belt adhesion structure of natural skin is accurately simulated, and the problems of epidermis-dermis layering and non-uniform cell infiltration of an existing skin flap are solved.

The utility model relates to the field of medical instruments, in particular to a newborn artificial skin puncher with a hole diameter adjusting function, which comprises a base, a sliding seat is slidably connected to the upper end of the base, the sliding seat is driven by a driving component to move up and down, and a switching block is connected to the center in the sliding seat through a rotating rod. A cutting component is arranged on the periphery of the switching block in the circumferential direction, the bottom face of the sliding base is connected with a pressing plate through a buffering component, a through groove is formed in the lower portion of the base, a switching block is rotationally connected into the through groove, and cutting holes matched with the cutting component are formed in the periphery of the switching block in the circumferential direction. The device is provided with the switching block, the dividing column, the conversion block and the like, and effectively solves the problem that the aperture is inconvenient to adjust when the artificial skin of the newborn is manufactured at present.

The application discloses a hydrogel based on a mechanical interlocking principle, and a preparation method and application thereof. The hydrogel is composed of a flexible high polymer main chain and a rigid short side chain. The flexible high polymer main chain is used as the main body, and the rigid short side chain is added into the flexible high polymer chain as a mechanical interlocking unit. The super-strong, super-tough and crack growth resistant hydrogel material based on the mechanical interlocking structure can be applied to soft robots, artificial skin, flexible electronic devices, artificial tendons, cartilage repair and regeneration. The rigid polypeptide in the application has a single end connected with acrylic acid to provide an unsaturated carbon-carbon double bond for participating in copolymerization.

The invention provides a medical instrument skin stimulation test data processing method based on an artificial skin model, and relates to the technical field of skin stimulation tests. Collecting the original OD value of each pore in the 96-pore plate under the wavelength of 570nm; calculating the survival rate and the standard deviation of the positive control group and the test sample group; verifying the data validity of the negative control group and the positive control group; and performing skin irritation judgment according to the average survival rate of the test sample group to obtain a skin irritation type. According to the method, the in-vitro skin model based on HTWSkinRHE is established, strict standardized culture conditions and grouping design are adopted, the batch-to-batch consistency and data comparability of the experimental model are remarkably improved, and the model can better meet more medical instrument requirements; the cell activity is detected by simulating a complete skin layered structure and combining an MTT method, and the judgment threshold value is highly matched with the in-vivo stimulation response.

Disclosed are an artificial muscle module, artificial skin implementing a micro-expression, and a manufacturing method therefor. The artificial muscle module of the present invention comprises: an outer coil including a coiled outer yarn having a hollow; and an inner elastic body located inside the hollow and including an elastic body. Through the introduction of a tension supporter, free-standing is possible without an initial load, resulting in high utilization and enabling implementation of micro-emotional expressions.

The invention discloses a high-efficiency anti-infection double-layer artificial skin and a preparation method thereof. The high-efficiency anti-infection double-layer artificial skin has an excellent antibacterial effect and can reduce the occurrence of mucous membrane and instrument related infections. The collagen scaffold is a recombinant collagen scaffold with a three-dimensional pore structure, provides a suitable physical structure for cells, is beneficial to adhesion and proliferation of fibroblasts, vascular endothelial cells and the like, and has a remarkable skin wound repair effect. According to the invention, mTGase is mainly used for cross-linking glutamine and lysine sites to form protein-protein cross-linking; the Bis-PEG6-Acid can be used for cross-linking the Lys (Lys-NH2) and another protein, so as to form PEG (Polyethylene Glycol)-protein cross-linking; the mTGase and the Bis-PEG6-Acid do not directly and synergistically catalyze the same reaction site, a primary protein network is firstly constructed by the mTGase, the crosslinking density is further enhanced by the Bis-PEG6-Acid, a double-network structure is realized, and the mechanical strength, the degradation resistance and the hydrophilicity of the stent material are enhanced. And the hydrogel also has good tensile elasticity and biocompatibility, and is free of cytotoxicity and sensitization.

The utility model discloses a double-blind acupuncture placebo needle for clinical tests, which relates to the technical field of medical experimental instruments and is characterized in that one end of a needle body is a passivated needle tip (skin contact end), the other end of the needle body is a sharpened needle tip (connected with a needle handle) capable of puncturing artificial skin gel, and the sharpened end of the needle tip is nested in a hollow needle handle. The needle body can slide in the needle body containing hole channel of the needle handle when being subjected to external force, the needle body containing hole channel is filled with the gel artificial skin capable of simulating the acupuncture hand feeling, the needle point can pierce the gel artificial skin when being subjected to external force, and therefore the hand feeling during acupuncture is simulated, and a needle applicator is covered. The technical problem that double blindness cannot be completed due to the fact that an existing placebo needle cannot simulate the real needle inserting hand feeling is solved; and the passivated needle head can be used for stabbing without stabbing into a subject, so that the subject feels painful, and whether the subject is a comforting needle or not cannot be distinguished, and therefore, double-blind operation can be smoothly completed.

The invention relates to the field of polymer composite materials, and discloses a hollow glass nano-microsphere modified organic silicon elastomer and a preparation method thereof. The material is prepared from the following components in percentage by mass: 60-90% of vinyl silicone oil, 0.1-10% of hydrogen-containing silicone oil, 1-20% of an MQ silicon resin solution, 1-50 ppm of a catalyst, 0.05-0.3% of an inhibitor, 5-20% of a solvent and 0.1-5% of hollow glass nano-microsphere turbid liquid, the m / q value of MQ silicon resin is 0.5-1.0, and hollow glass nano-microspheres are silicon dioxide hollow submicrospheres. The preparation method comprises the following steps: mixing the raw materials, adding a catalyst, hydrogen-containing silicone oil and an inhibitor into two groups, mixing, defoaming, curing and forming. The material has good flexibility, ductility, air permeability and moisture permeability, high thermal stability and excellent biocompatibility, and is suitable for the fields of artificial skin and the like.

The invention provides macroporous structure eutectic hydrogel as well as a preparation method and application thereof, and belongs to the technical field of hydrogel preparation. The preparation method comprises the following steps: firstly preparing an MXene coated MWCNTs-NH2 dispersion liquid, then adding PVA and a pore-forming agent into the MXene coated MWCNTs-NH2 dispersion liquid to obtain a mixed liquid, then grinding the mixed liquid to obtain a pre-gel liquid, then carrying out circulating freezing-unfreezing on the pre-gel liquid, then soaking in an acidic solution to obtain a macroporous hydrogel, and finally, carrying out ultrasonic treatment on the macroporous hydrogel to obtain the porous hydrogel. Soaking the macroporous hydrogel in a mixed solution of a deep eutectic solvent and deionized water for solvent replacement, and obtaining the macroporous hydrogel after soaking; the prepared macroporous structure eutectic hydrogel has excellent mechanical property, conductivity, antibacterial property and environmental stability; the method can be applied to preparation of wearable sensors or intelligent artificial skins, and the prepared sensors have excellent sensitivity.

The application is a preparation of a composite stem cell active biomaterial and its application in artificial skin, effectively solves the problems of high production cost, complex production process and non-use for active substance research of the existing method, and comprises a double-layer composite material, the lower layer is an active material layer in the form of hydrogel composed of active liposomes and stem cells, and the upper layer is an antibacterial layer; the preparation method of the thiolated hyaluronic acid / H active factor composite hydrogel is relatively mild, does not need to add a crosslinking agent and contains a large amount of active ingredients; by using the mechanism, the environment can induce angiogenesis through endothelial cells / fat stem cells combined with active factors and platelet lysate and rapidly construct microtissues in vitro, which is beneficial to the scaffold in large-scale cultivation of artificial skin basement layer in vitro to meet the needs of patients.

This invention belongs to the field of biomedical materials, specifically providing a highly permeable hydrophilic polyurethane artificial membrane based on chemical grafting and its preparation method, comprising the following steps: S1 - Substrate pretreatment: Immersing a polyurethane film in an organic solvent, ultrasonically cleaning and soaking for 0.5-2 hours, then removing and drying to constant weight to obtain a pretreated substrate; S2 - Preparation of hydrophilic modified polyurethane: Completely immersing the substrate obtained in step S1 in a hydrophilic modification solution, introducing an inert gas for 10-30 minutes, heating to 35-45°C, and reacting under stirring or shaking in the dark for 6-24 hours to obtain a hydrophilic modified polyurethane membrane; S3 - Post-treatment: The hydrophilic modified polyurethane membrane from step S2 is sequentially acid-washed, water-washed, and then vacuum-dried to obtain the hydrophilic polyurethane artificial membrane. The hydrophilic polyurethane artificial membrane of this invention, prepared by chemical grafting, possesses high permeability and excellent mechanical properties.

The invention discloses a silk material with a function of promoting cell proliferation and application of the silk material. According to the functional material, a human-derived I-type collagen truncated fragment is expressed by a silkworm middle silk gland, and a sericin promoter serving as a tissue-specific promoter can drive an exogenous gene to be specifically expressed in the middle silk gland; finally, the silkworm cocoons are combined with sericin to be secreted in vitro, foreign protein can be separated after certain treatment is carried out on the silkworm cocoons, and the expression product can maintain biological activity. The hydrogel prepared from the silk material has the effect of promoting wound healing, and can be applied to the aspects of promoting skin regeneration and repair, bone transplantation substitutes, cartilage reconstruction, artificial skin and the like.

1. The name of the design product: insulin injection simulation artificial skin. 2. The use of the design product: the product is mainly used to demonstrate injection site selection and injection process in subcutaneous injection education process. 3. The design points of the design product: the combination of shape, pattern and color. 4. The picture or photo that best indicates the design points: perspective view. 5. The design for which protection is sought contains color. 6. The back of the design product is a part that is not easy to see or cannot be seen during use, and the rear view is omitted.

This utility model discloses a quantitative measuring device for evaluating the stickiness of cosmetics, belonging to the field of cosmetic testing technology. It includes a fixing component, a telescopic component, a measuring component, a pressure block, and test skin. The fixing component includes a base and a column; the base is placed on a working surface; the column is mounted on the base; the telescopic component is mounted on the base; the measuring component includes a connector and a force sensor; the force sensor is mounted on the column; the connector is connected to the force sensor; the test skin includes a first skin and a second skin, which are in contact with each other; the pressure block is used to enhance the contact between the first and second skins; the first skin is mounted on the telescopic component, and the second skin is connected to the connector. This utility model uses artificial skin to simulate the contact and separation process of skin, making operation simple and reducing the influence of subjective factors and external environment on the evaluation results; by measuring the force during the skin separation process through the force sensor, it achieves intuitive quantitative evaluation.

The invention provides antibacterial hydrogel artificial skin and a preparation method thereof, and belongs to the technical field of 3D printing biomaterials, the antibacterial hydrogel artificial skin is prepared from the following raw materials by weight: 1-3 parts of aluminum chloride hexahydrate, 30-45 parts of water, 15-22 parts of graft modified chitosan, 8-15 parts of acrylamide, and 1-3 parts of N, N-dimethylformamide. The coating comprises the following components in parts by weight: 0.2-0.8 part of N, N '-methylene bisacrylamide, 0.5-1.5 parts of a photoinitiator, 6-12 parts of quaternized chitosan and 4-8 parts of nano-hydroxyapatite. Wherein the graft modified chitosan is prepared from the following raw materials: 5 to 10 parts of chitosan, 80 to 120 parts of acetic acid solution with the concentration of 2 percent, 0.8 to 2.5 parts of glycidyl methacrylate and 0.05 to 0.2 part of azodiisobutyronitrile. According to the application, by adopting a formula taking the grafted modified chitosan as a core, a collaborative design of multi-scale crosslinking and personalized 3D printing is formed, high mechanical strength, broad-spectrum long-acting bacteriostasis, precise form adaptation and excellent biocompatibility of the hydrogel are realized, and the clinical complex wound repair requirements are met.

The invention provides a polarization imaging-based automatic detection method for preventing cheating of a camouflage mask. The method comprises the following steps of: acquiring and constructing data; preprocessing and labeling the data; training a preset model; calculating a detection frame coordinate, a category label and a confidence coefficient; and defining a camouflage tendency value according to the category label and the confidence coefficient, and judging the authenticity of the detected target according to the camouflage tendency value and a preset judgment threshold value. According to the method, the polarization reflection characteristics are utilized to construct the face surface polarization characteristics, and the distinguishability of different materials (such as silica gel, artificial skin and three-dimensional printing materials) in the microstructure and the reflection mode is enhanced by calculating the linear polarization degree and the polarization angle and carrying out energy weighted fusion.

The application relates to an apparatus which comprises at least one portion of artificial skin (103) and a plurality of sensors (105) dispersed within the at least one portion of artificial skin (103). The plurality of sensors (105) have at least one physical property which is configured to be modified when the plurality of sensors (105) are exposed to a parameter such that the modification of the physical property can be detected by an external detector (211). The application also relates to detection systems comprising such apparatus.

The invention relates to the field of tissue engineering biological materials, in particular to an epidermal stem cell-loaded imitation skin ridge microneedle and a preparation method and application thereof, and the method comprises the following steps: providing a target skin sample; a three-dimensional structure of the target skin sample is obtained through laser femtosecond three-dimensional reconstruction, image processing software is adopted for analysis and quantification, and skin ridge size parameters of the target skin sample are obtained; preparing a microneedle mold according to the skin ridge size parameters; respectively preparing a needle tip solution and a substrate solution, and adding the epidermal stem cells in the culture logarithmic phase into the needle tip solution to obtain a mixed solution; filling a microneedle area of a microneedle mold with the mixed solution, and performing centrifugal treatment; and filling a substrate area of a microneedle mold with the substrate solution, carrying out illumination crosslinking, and demolding to obtain the epidermal stem cell-loaded artificial skin ridge microneedle. The method can realize stable survival of epidermal stem cells in the artificial skin ridge microneedle, and has the effects of reconstructing a skin wound skin ridge structure and improving wound healing quality.

The application discloses a silk material with a cell proliferation function and application thereof, the functional material is expressed by a human type I collagen truncated fragment in a middle silk gland of a domestic silkworm, a sericin promoter is used as a tissue-specific promoter to drive specific expression of an exogenous gene in the middle silk gland, and finally the exogenous gene is combined with sericin protein and is secreted to outside of the body, the exogenous protein can be separated after a certain treatment of a cocoon, and the expression product can keep biological activity. The silk material is used to prepare a hydrogel, has a function of promoting wound healing, and can be applied to aspects of promoting skin regeneration and repair, bone graft substitutes, cartilage reconstruction, artificial skin and the like.

Disclosed is an artificial adipose tissue comprising a three-dimensional tissue comprising: a population of cells including an adipocyte; and a fragmented extracellular matrix.

The present invention provides a skin in-situ printing device, which is used for applying biological glue for forming artificial skin by printing on a predetermined part of a human body, and comprises: a carrying device; a plurality of print head devices; a coating power device; a print head moving device; and a control device, wherein the print head device comprises: a printing unit, which is used for accommodating and applying different biological glues or biological glue components, and has a cylinder for accommodating the biological glue and a needle for applying the biological glue arranged at the bottom of the cylinder; a printing lifting component, which is used for lifting the printing unit, and has a lifting groove, a lifting screw arranged in the lifting groove, a lifting ball bearing which is sleeved on the lifting screw through a threaded combination to form a ball screw pair with the lifting screw, and a lifting drive motor; a printing moving component, which is used for horizontally moving the printing lifting unit, so that the printing unit moves horizontally; and a printing bracket, which has a mounting portion, a connecting portion and a accommodating portion.

The present application relates to the technical field of biomedical materials, in particular to a super-elastic hydrogel material, a super-elastic injectable scaffold and a preparation method and application thereof. The super-elastic hydrogel material is obtained by cross-linking a base material with an initiator; the base material comprises GelMA and PEGDA with a molecular weight of 2000-20000; the injectable scaffold comprises a three-dimensional structure scaffold composed of super-elastic material, which can restore the original shape after being compressed and injected. The present application utilizes the excellent mechanical properties and shape memory of super-elastic material to realize solid injection and restore the original shape after injection, thereby providing a new idea and approach for large-size injectable scaffolds. The scaffold of the present application can be used in the fields of tissue engineering and regenerative medicine, minimally invasive injection transplantation, artificial skin, myocardial patch, in-vitro engineered tissue model construction, tissue physiological function remodeling and pathological process mechanism research, mechanical regulation and stress regulation mechanism research, drug development and drug screening, etc.

To provide a culture insert container that reduces an amount of fibroblast cells used, eliminates the need of a hollowing process, improves yield and eliminates various processes requiring proficiency, to dramatically increase production efficiency, and to provide a method for producing tension-applied three-dimensional artificial skin employing the same.SOLUTION: The present invention provides a culture insert container having a lower culture container having a bottom portion equipped with a porous membrane and a sidewall, and having an upper insertion fixture having a cylindrical sidewall that is inserted into the lower culture container, wherein the cylindrical sidewall has a land arranged so as to protrude from a top end of the sidewall of the lower culture container at a location so as to cause a top surface of the porous membrane and a bottom end portion of the cylindrical sidewall which is inserted within the lower culture container to be maintained in a non-contact state, and a land arranged at an outer surface of the cylindrical sidewall so as to cause a constant distance to be maintained between an inner surface of the sidewall of the lower culture container and the outer surface of the cylindrical sidewall.SELECTED DRAWING: Figure 9

The application discloses a multifunctional gradient structure artificial skin and a preparation method and application thereof, and belongs to the technical field of biomaterials and biomedical engineering. The upper layer of the application is a silica gel layer, which is used for simulating epidermis to keep a moist environment of a wound and simultaneously plays a barrier role; the intermediate layer is a degradable polymer / natural bioactive protein composite layer, which is used for enhancing mechanical properties; and the lower layer is a hydrogel layer, which is formed by cross-linking hyaluronic acid and carboxymethyl chitosan to form a Schiff base bond and then cross-linking collagen through catalysis. The composition of the hydrogel layer is a component of an extracellular matrix, and the three-dimensional network structure formed is beneficial to cell growth, can simulate a dermis layer to play a function of promoting tissue regeneration and remodeling, and finally the artificial skin with good mechanical strength, capable of preventing wound infection and promoting wound healing is obtained.