70 results about "Artificial skin" patented technology

The invention provides a recombinant humanized elastin with high biological activity as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering and biological materials. 100% of the amino acid sequence of the elastin is derived from human elastin and is prepared by expression of genetically engineered bacteria; the protein has a typical beta-spiral hydrophobic structural domain and a lysine-rich hydrophilic structural domain, has a good bionic structure and functional characteristics, can significantly promote proliferation, adhesion and migration of human skin fibroblasts, also has excellent antioxidant, anti-wrinkle and anti-aging activities, and can be used for preparing a biomimetic protein. The hydrogel can be widely applied to the fields of skin repair dressings, injection implants, artificial skin, biomedical materials, functional skin care products and the like; in addition, the elastin is good in solubility, high in stability, mature in expression system and suitable for large-scale production.

The invention relates to artificial skin, in particular to self-generating full-layer artificial skin for wound treatment and application of the self-generating full-layer artificial skin. The artificial epidermis has an epidermis-dermis structure similar to skin, and the artificial epidermis structure is composed of an electrostatic spinning nano-film with piezoelectric capacity and has the functions of sterilization, protection and bioelectricity generation of an epidermis layer; the artificial dermal structure is composed of a photo-crosslinking polysaccharide and enzyme crosslinking protein interpenetrating hydrogel skeleton, by adding bioactive components and loading living cells, the effects of releasing active components and cytokines, remodeling wound extracellular matrix and promoting cell proliferation are achieved, and finally healing of acute and chronic wounds is remarkably accelerated. The invention provides a simple, feasible, economical and effective treatment method for sealing a large-area wound, sterilizing the wound and promoting healing of the wound.

The invention provides a preparation method of a composite artificial flap with a bionic structure and function induction activity. The preparation method comprises the following steps: S1, preparing an epidermis induction layer; s2, preparing a dermis functional remodeling layer; s3, preparation of a vascularization integration substrate layer; and S4, three-layer compounding and sterilization. Through an epidermis-basilar membrane-dermis three-layer integrated structure, a micron-nano multistage gradient pore and basilar membrane belt adhesion structure of natural skin is accurately simulated, and the problems of epidermis-dermis layering and non-uniform cell infiltration of an existing skin flap are solved.

The utility model relates to the field of medical instruments, in particular to a newborn artificial skin puncher with a hole diameter adjusting function, which comprises a base, a sliding seat is slidably connected to the upper end of the base, the sliding seat is driven by a driving component to move up and down, and a switching block is connected to the center in the sliding seat through a rotating rod. A cutting component is arranged on the periphery of the switching block in the circumferential direction, the bottom face of the sliding base is connected with a pressing plate through a buffering component, a through groove is formed in the lower portion of the base, a switching block is rotationally connected into the through groove, and cutting holes matched with the cutting component are formed in the periphery of the switching block in the circumferential direction. The device is provided with the switching block, the dividing column, the conversion block and the like, and effectively solves the problem that the aperture is inconvenient to adjust when the artificial skin of the newborn is manufactured at present.

The application discloses a hydrogel based on a mechanical interlocking principle, and a preparation method and application thereof. The hydrogel is composed of a flexible high polymer main chain and a rigid short side chain. The flexible high polymer main chain is used as the main body, and the rigid short side chain is added into the flexible high polymer chain as a mechanical interlocking unit. The super-strong, super-tough and crack growth resistant hydrogel material based on the mechanical interlocking structure can be applied to soft robots, artificial skin, flexible electronic devices, artificial tendons, cartilage repair and regeneration. The rigid polypeptide in the application has a single end connected with acrylic acid to provide an unsaturated carbon-carbon double bond for participating in copolymerization.

The invention provides a medical instrument skin stimulation test data processing method based on an artificial skin model, and relates to the technical field of skin stimulation tests. Collecting the original OD value of each pore in the 96-pore plate under the wavelength of 570nm; calculating the survival rate and the standard deviation of the positive control group and the test sample group; verifying the data validity of the negative control group and the positive control group; and performing skin irritation judgment according to the average survival rate of the test sample group to obtain a skin irritation type. According to the method, the in-vitro skin model based on HTWSkinRHE is established, strict standardized culture conditions and grouping design are adopted, the batch-to-batch consistency and data comparability of the experimental model are remarkably improved, and the model can better meet more medical instrument requirements; the cell activity is detected by simulating a complete skin layered structure and combining an MTT method, and the judgment threshold value is highly matched with the in-vivo stimulation response.

Disclosed are an artificial muscle module, artificial skin implementing a micro-expression, and a manufacturing method therefor. The artificial muscle module of the present invention comprises: an outer coil including a coiled outer yarn having a hollow; and an inner elastic body located inside the hollow and including an elastic body. Through the introduction of a tension supporter, free-standing is possible without an initial load, resulting in high utilization and enabling implementation of micro-emotional expressions.

The invention discloses a high-efficiency anti-infection double-layer artificial skin and a preparation method thereof. The high-efficiency anti-infection double-layer artificial skin has an excellent antibacterial effect and can reduce the occurrence of mucous membrane and instrument related infections. The collagen scaffold is a recombinant collagen scaffold with a three-dimensional pore structure, provides a suitable physical structure for cells, is beneficial to adhesion and proliferation of fibroblasts, vascular endothelial cells and the like, and has a remarkable skin wound repair effect. According to the invention, mTGase is mainly used for cross-linking glutamine and lysine sites to form protein-protein cross-linking; the Bis-PEG6-Acid can be used for cross-linking the Lys (Lys-NH2) and another protein, so as to form PEG (Polyethylene Glycol)-protein cross-linking; the mTGase and the Bis-PEG6-Acid do not directly and synergistically catalyze the same reaction site, a primary protein network is firstly constructed by the mTGase, the crosslinking density is further enhanced by the Bis-PEG6-Acid, a double-network structure is realized, and the mechanical strength, the degradation resistance and the hydrophilicity of the stent material are enhanced. And the hydrogel also has good tensile elasticity and biocompatibility, and is free of cytotoxicity and sensitization.

The utility model discloses a double-blind acupuncture placebo needle for clinical tests, which relates to the technical field of medical experimental instruments and is characterized in that one end of a needle body is a passivated needle tip (skin contact end), the other end of the needle body is a sharpened needle tip (connected with a needle handle) capable of puncturing artificial skin gel, and the sharpened end of the needle tip is nested in a hollow needle handle. The needle body can slide in the needle body containing hole channel of the needle handle when being subjected to external force, the needle body containing hole channel is filled with the gel artificial skin capable of simulating the acupuncture hand feeling, the needle point can pierce the gel artificial skin when being subjected to external force, and therefore the hand feeling during acupuncture is simulated, and a needle applicator is covered. The technical problem that double blindness cannot be completed due to the fact that an existing placebo needle cannot simulate the real needle inserting hand feeling is solved; and the passivated needle head can be used for stabbing without stabbing into a subject, so that the subject feels painful, and whether the subject is a comforting needle or not cannot be distinguished, and therefore, double-blind operation can be smoothly completed.

The invention relates to the field of polymer composite materials, and discloses a hollow glass nano-microsphere modified organic silicon elastomer and a preparation method thereof. The material is prepared from the following components in percentage by mass: 60-90% of vinyl silicone oil, 0.1-10% of hydrogen-containing silicone oil, 1-20% of an MQ silicon resin solution, 1-50 ppm of a catalyst, 0.05-0.3% of an inhibitor, 5-20% of a solvent and 0.1-5% of hollow glass nano-microsphere turbid liquid, the m / q value of MQ silicon resin is 0.5-1.0, and hollow glass nano-microspheres are silicon dioxide hollow submicrospheres. The preparation method comprises the following steps: mixing the raw materials, adding a catalyst, hydrogen-containing silicone oil and an inhibitor into two groups, mixing, defoaming, curing and forming. The material has good flexibility, ductility, air permeability and moisture permeability, high thermal stability and excellent biocompatibility, and is suitable for the fields of artificial skin and the like.

The application is a preparation of a composite stem cell active biomaterial and its application in artificial skin, effectively solves the problems of high production cost, complex production process and non-use for active substance research of the existing method, and comprises a double-layer composite material, the lower layer is an active material layer in the form of hydrogel composed of active liposomes and stem cells, and the upper layer is an antibacterial layer; the preparation method of the thiolated hyaluronic acid / H active factor composite hydrogel is relatively mild, does not need to add a crosslinking agent and contains a large amount of active ingredients; by using the mechanism, the environment can induce angiogenesis through endothelial cells / fat stem cells combined with active factors and platelet lysate and rapidly construct microtissues in vitro, which is beneficial to the scaffold in large-scale cultivation of artificial skin basement layer in vitro to meet the needs of patients.

This invention belongs to the field of biomedical materials, specifically providing a highly permeable hydrophilic polyurethane artificial membrane based on chemical grafting and its preparation method, comprising the following steps: S1 - Substrate pretreatment: Immersing a polyurethane film in an organic solvent, ultrasonically cleaning and soaking for 0.5-2 hours, then removing and drying to constant weight to obtain a pretreated substrate; S2 - Preparation of hydrophilic modified polyurethane: Completely immersing the substrate obtained in step S1 in a hydrophilic modification solution, introducing an inert gas for 10-30 minutes, heating to 35-45°C, and reacting under stirring or shaking in the dark for 6-24 hours to obtain a hydrophilic modified polyurethane membrane; S3 - Post-treatment: The hydrophilic modified polyurethane membrane from step S2 is sequentially acid-washed, water-washed, and then vacuum-dried to obtain the hydrophilic polyurethane artificial membrane. The hydrophilic polyurethane artificial membrane of this invention, prepared by chemical grafting, possesses high permeability and excellent mechanical properties.

1. The name of the design product: insulin injection simulation artificial skin. 2. The use of the design product: the product is mainly used to demonstrate injection site selection and injection process in subcutaneous injection education process. 3. The design points of the design product: the combination of shape, pattern and color. 4. The picture or photo that best indicates the design points: perspective view. 5. The design for which protection is sought contains color. 6. The back of the design product is a part that is not easy to see or cannot be seen during use, and the rear view is omitted.

This utility model discloses a quantitative measuring device for evaluating the stickiness of cosmetics, belonging to the field of cosmetic testing technology. It includes a fixing component, a telescopic component, a measuring component, a pressure block, and test skin. The fixing component includes a base and a column; the base is placed on a working surface; the column is mounted on the base; the telescopic component is mounted on the base; the measuring component includes a connector and a force sensor; the force sensor is mounted on the column; the connector is connected to the force sensor; the test skin includes a first skin and a second skin, which are in contact with each other; the pressure block is used to enhance the contact between the first and second skins; the first skin is mounted on the telescopic component, and the second skin is connected to the connector. This utility model uses artificial skin to simulate the contact and separation process of skin, making operation simple and reducing the influence of subjective factors and external environment on the evaluation results; by measuring the force during the skin separation process through the force sensor, it achieves intuitive quantitative evaluation.

The invention provides antibacterial hydrogel artificial skin and a preparation method thereof, and belongs to the technical field of 3D printing biomaterials, the antibacterial hydrogel artificial skin is prepared from the following raw materials by weight: 1-3 parts of aluminum chloride hexahydrate, 30-45 parts of water, 15-22 parts of graft modified chitosan, 8-15 parts of acrylamide, and 1-3 parts of N, N-dimethylformamide. The coating comprises the following components in parts by weight: 0.2-0.8 part of N, N '-methylene bisacrylamide, 0.5-1.5 parts of a photoinitiator, 6-12 parts of quaternized chitosan and 4-8 parts of nano-hydroxyapatite. Wherein the graft modified chitosan is prepared from the following raw materials: 5 to 10 parts of chitosan, 80 to 120 parts of acetic acid solution with the concentration of 2 percent, 0.8 to 2.5 parts of glycidyl methacrylate and 0.05 to 0.2 part of azodiisobutyronitrile. According to the application, by adopting a formula taking the grafted modified chitosan as a core, a collaborative design of multi-scale crosslinking and personalized 3D printing is formed, high mechanical strength, broad-spectrum long-acting bacteriostasis, precise form adaptation and excellent biocompatibility of the hydrogel are realized, and the clinical complex wound repair requirements are met.

The invention provides a polarization imaging-based automatic detection method for preventing cheating of a camouflage mask. The method comprises the following steps of: acquiring and constructing data; preprocessing and labeling the data; training a preset model; calculating a detection frame coordinate, a category label and a confidence coefficient; and defining a camouflage tendency value according to the category label and the confidence coefficient, and judging the authenticity of the detected target according to the camouflage tendency value and a preset judgment threshold value. According to the method, the polarization reflection characteristics are utilized to construct the face surface polarization characteristics, and the distinguishability of different materials (such as silica gel, artificial skin and three-dimensional printing materials) in the microstructure and the reflection mode is enhanced by calculating the linear polarization degree and the polarization angle and carrying out energy weighted fusion.

The application discloses a silk material with a cell proliferation function and application thereof, the functional material is expressed by a human type I collagen truncated fragment in a middle silk gland of a domestic silkworm, a sericin promoter is used as a tissue-specific promoter to drive specific expression of an exogenous gene in the middle silk gland, and finally the exogenous gene is combined with sericin protein and is secreted to outside of the body, the exogenous protein can be separated after a certain treatment of a cocoon, and the expression product can keep biological activity. The silk material is used to prepare a hydrogel, has a function of promoting wound healing, and can be applied to aspects of promoting skin regeneration and repair, bone graft substitutes, cartilage reconstruction, artificial skin and the like.

Disclosed is an artificial adipose tissue comprising a three-dimensional tissue comprising: a population of cells including an adipocyte; and a fragmented extracellular matrix.

The present application relates to the technical field of biomedical materials, in particular to a super-elastic hydrogel material, a super-elastic injectable scaffold and a preparation method and application thereof. The super-elastic hydrogel material is obtained by cross-linking a base material with an initiator; the base material comprises GelMA and PEGDA with a molecular weight of 2000-20000; the injectable scaffold comprises a three-dimensional structure scaffold composed of super-elastic material, which can restore the original shape after being compressed and injected. The present application utilizes the excellent mechanical properties and shape memory of super-elastic material to realize solid injection and restore the original shape after injection, thereby providing a new idea and approach for large-size injectable scaffolds. The scaffold of the present application can be used in the fields of tissue engineering and regenerative medicine, minimally invasive injection transplantation, artificial skin, myocardial patch, in-vitro engineered tissue model construction, tissue physiological function remodeling and pathological process mechanism research, mechanical regulation and stress regulation mechanism research, drug development and drug screening, etc.

The application discloses a multifunctional gradient structure artificial skin and a preparation method and application thereof, and belongs to the technical field of biomaterials and biomedical engineering. The upper layer of the application is a silica gel layer, which is used for simulating epidermis to keep a moist environment of a wound and simultaneously plays a barrier role; the intermediate layer is a degradable polymer / natural bioactive protein composite layer, which is used for enhancing mechanical properties; and the lower layer is a hydrogel layer, which is formed by cross-linking hyaluronic acid and carboxymethyl chitosan to form a Schiff base bond and then cross-linking collagen through catalysis. The composition of the hydrogel layer is a component of an extracellular matrix, and the three-dimensional network structure formed is beneficial to cell growth, can simulate a dermis layer to play a function of promoting tissue regeneration and remodeling, and finally the artificial skin with good mechanical strength, capable of preventing wound infection and promoting wound healing is obtained.

The invention belongs to the technical field of artificial skin preparation, and particularly relates to a system and method for preparing artificial skin through 3D printing, the system integrates key steps of cell extraction, amplification, artificial skin preparation and culture and the like, rapid amplification of skin cells and efficient construction of the artificial skin are achieved, the overall efficiency is remarkably improved, and the cost is reduced. The preparation period is shortened; the integrated square cabin system provided by the invention can move, so that the timeliness of cell extraction is ensured; besides, the prepared artificial skin with the net ridge structure has a certain thickness, the upper surface and the lower surface are flat, and the artificial skin has a wavy staggered three-dimensional structure, so that more epidermal stem cell dryness can be reserved, and the maintenance of epidermal stem cell marker protein is facilitated.

The application discloses a kind of based on artificial leather's leakage testing device for indwelling needle, it is related to the leakage testing field of indwelling needle, including base, the top of base is rotatably connected with carousel, carousel top is equipped with device body, the center of device body top is equipped with leakage testing assembly, the top of leakage testing assembly is equipped with mounting column, mounting column outer wall is equipped with air bag, the top of leakage testing assembly is provided with artificial leather displacement assembly, one end of artificial leather displacement assembly is rotatably connected with cam, the bottom of artificial leather displacement assembly is movably connected with artificial leather, and the top of artificial leather is equipped with connecting column.The leakage testing device for indwining needle based on artificial leather of the application, by integrating automation flow, pneumatic seal, bionic material fitting, precision mechanical transmission and sensing monitoring technology, traditional inefficient, inaccurate manual leak detection method is successfully replaced, can effectively improve the efficiency, accuracy, consistency, health standard and of indwining needle leak detection.

The present invention discloses a microscopic observation preparation method based on Gastrodia elata powder and an application thereof, and relates to the technical field of Gastrodia elata powder preparation. The following scheme is proposed, comprising the following steps: S1, weighing: taking out an electronic scale, grabbing an appropriate amount of dry Gastrodia elata slices for weighing, S2, crushing: putting the weighed Gastrodia elata slices into a powder preparation device for crushing; the method performs microscopic observation on Gastrodia elata powder, and can identify and judge the quality of Gastrodia elata from various aspects, avoiding deviation in identification results caused by accidental or human factors, and ensuring the quality of Gastrodia elata. The method also assists in preparation through a heating mechanism, avoiding the situation where the solution is spilled or difficult to spread during heating, resulting in difficulty in permeabilization, and no manual hand-holding is required during permeabilization, saving time and effort, and avoiding the situation of artificial skin burns, thereby increasing the preparation speed, saving a lot of preparation time, improving the preparation efficiency of Gastrodia elata powder, and making identification more convenient.

According to the technical scheme, the artificial skin dialysis detection equipment for the medical dermatology department is characterized by comprising a base, a fixing plate is fixedly installed on the top face of the base, a rotating table is arranged on the top face of the fixing plate, an adjusting hole is formed in the top face of the fixing plate, the rotating table is movably connected with the adjusting hole in a sleeved mode, and the rotating table is movably connected with the adjusting hole in a sleeved mode. A support frame is fixedly mounted on the top surface of the fixed plate; the detection assembly is arranged in the supporting frame and used for detecting the permeability of the artificial skin, a base, a fixing plate, a rotating table, the supporting frame, a storage box, a distinguishing plate, a connecting pipe, a transition box, a mounting plate, a fixing pipe, a spray head and a telescopic pipe which are arranged are used in cooperation, fluorescent emulsion is smeared on the artificial skin, the emulsion can permeate into the artificial skin, and the permeability of the artificial skin is detected. And the fluorescent agent enters the artificial skin and generates obvious fluorescent bright spots or areas under the irradiation of ultraviolet rays, so that a tiny permeation phenomenon can be accurately detected.

The invention provides a multilayer artificial skin flap containing a built-in nutritional blood vessel and a preparation method of the multilayer artificial skin flap, and belongs to the technical field of tissue engineering and regenerative medicine. The artificial skin flap is sequentially provided with a first decellularized meat pulp matrix layer, a nutritional blood vessel, a fabric layer and a second decellularized meat pulp matrix layer from top to bottom. The nutrition blood vessel can construct a nutrition delivery channel for surrounding tissues, and promote rapid proliferation of cells and formation of a new blood vessel network; the decellularized meat pulp matrix layer provides a bionic microenvironment and supports cell adhesion, migration and growth; the fabric layer plays a role in enhancing mechanical support and simultaneously endows the flap with good stitchability and tear resistance; in addition, the nutritional blood vessel and the fabric layer are both made of degradable filaments, the degradation period can be regulated and controlled according to needs, and space and mechanical support smooth transition is vacated for tissue growth. The artificial skin flap has excellent biocompatibility, controllable degradability, active vascularization ability and proper mechanical properties, and is suitable for full-thickness skin defect repair and soft tissue regeneration engineering.

This invention provides a highly efficient transdermal small molecular weight recombinant transdermal human elastin, its preparation method, and its applications, belonging to the fields of genetic engineering and biomaterials technology. The amino acid sequence of the elastin is derived from human elastin, with a His tag at its N-terminus for purification, and a TD1 transdermal peptide fused to its C-terminus to enhance transdermal delivery. The elastin is prepared through expression in genetically engineered bacteria, has a small molecular weight, and possesses a typical β-helical hydrophobic domain, exhibiting excellent biomimetic structure and functional properties. The small molecular weight recombinant transdermal human elastin demonstrates high transdermal efficiency and excellent biocompatibility, and can be widely used in skin repair dressings, implants, artificial skin, biomedical materials, and functional skincare products. Furthermore, the elastin exhibits good solubility, high stability, and a mature expression system, making it suitable for large-scale production.

The invention discloses a preparation method of exosome-loaded enzyme response type sandwich bionic artificial skin and application of the exosome-loaded enzyme response type sandwich bionic artificial skin in diabetic wounds. According to the prepared enzyme response type bionic artificial skin, hypoxia exosomes prepared from hypoxia stimulated cells are loaded through a fiber membrane; the engineering exosome of the high-load chemotactic factor CCL-2 is prepared by self-assembled polypeptide gel loading and inflammatory factor stimulation, and the two exosomes are combined to regulate and control the immune microenvironment of the diabetic wound surface so as to better promote the healing of the diabetic wound surface.

The present disclosure provides a care device with improved pleasantness when the care device is brought into contact with and worn on the skin. The care device of the present disclosure is a care device that is brought into contact with or worn on the skin. The care device of the present disclosure includes a skin wearing member containing an elastomer. The skin wearing member has an Asker C hardness of 30 degrees or less and a coefficient of static friction of 3 or less. Here, the coefficient of static friction is a coefficient of static friction for an artificial skin sheet that satisfies the following characteristics (A) and (B). (A) A compressive stress of the artificial skin sheet at a time of being pushed in a thickness direction at a depth of 2 mm is between 0.01 MPa and 0.05 MPa inclusive. (B) Calculated average roughness Ra of a surface of the artificial skin sheet is from 5 μm to 10 μm.

The application belongs to the technical field of bionic skin material preparation, and specifically provides a silicone and water-based polyurethane composite bionic skin material and a preparation method thereof. The material is prepared by compounding a water-based polyurethane synthetic leather, human silicone and a conductive fiber cloth base. The water-based polyurethane material of the epidermis gives the material the touch of baby skin; the middle silicone material acts as a muscle layer, ensuring that the overall material has a soft and elastic meat feeling; the conductive fiber cloth at the lowermost layer can not only act as a heating system, but also provide a linkage between the skin and the core control mechanism of the robot as a wire. This process not only solves the problem of low surface energy of silicone and difficulty in gluing, but also retains the soft and elastic meat feeling of silicone and gives it the touch of baby skin. The material has the advantages of baby skin touch, high strength and good durability, so the flexible robot skin material will have a broad application prospect in the fields of artificial skin, soft robot, wearable device, soft toy and prosthesis epidermis.

The present application relates to the field of biological materials, in particular to a high-efficiency transdermal recombinant humanized elastin as well as a preparation method and application thereof. In view of the problem that macromolecular drugs and proteins are difficult to be effectively absorbed through skin in the prior art, the present application discloses a high-efficiency transdermal recombinant humanized elastin, a coding gene, a recombinant vector, a recombinant genetically engineered bacterium, and a preparation method and application thereof. The protein has cell penetration ability and transdermal ability, and is obtained by protease cleavage from a precursor elastin. The nucleotide sequence of the coding gene of the precursor elastin is a sequence shown in SEQ ID NO. 3 or an equivalent sequence. The recombinant humanized elastin of the present application can be applied to skin care products, dressings, implants, artificial skin, artificial blood vessels, medical devices, biological materials or functional foods, and is particularly suitable for external preparations for anti-wrinkle, anti-aging or skin barrier repair.