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Antheraea pernyi silk fibrion biology medicine material and the preparation method

A technology of tussah silk fibroin and biomedical materials, which is applied in the field of biomedical materials and can solve the problems of inability to meet the requirements of three-dimensional stent shaping and mechanical properties.

Inactive Publication Date: 2007-09-19
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the molecular weight is too small, it cannot meet the requirements for the preparation of three-dimensional scaffolds for biomedical materials and mechanical properties.

Method used

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  • Antheraea pernyi silk fibrion biology medicine material and the preparation method
  • Antheraea pernyi silk fibrion biology medicine material and the preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Put 0.6 kg of leftover tussah silk (a by-product of a silk reeling factory) into 10 liters of 0.05% sodium carbonate aqueous solution, boil for 0.5 hours, repeat the treatment three times, and remove the sericin around the fibroin to obtain pure tussah silk white;

[0023] 2. Put the pure tussah silk fibroin after natural drying into a ternary solution with a weight ratio of lithium thiocyanate: mercaptoethanol: urea of ​​80:5:5 by weight, at a temperature of 60°C Dissolved under high temperature for 20 hours, purified by filtration and ultrafiltration, ultrasonic defoaming for 1 hour, and dried to make pure tussah silk fibroin;

[0024] 3. Put the pure tussah silk fibroin protein in formic acid solution, dissolve it for 4 hours at a temperature of 30°C, the final concentration of the solution is 10%, filter, purify, and defoam to make spinning raw materials;

[0025] 4. Using the electrospinning method, the distance from the receiving plate to the spinneret is 10cm...

Embodiment 2

[0028] 1. Put 1 kg of cocoon layer into 20 liters of neutral soap solution with a concentration of 0.5%, boil it for 2 hours, remove the sericin around the silk fibroin, wash it thoroughly with hot water to obtain pure tussah silk fibroin;

[0029] 2. Place the dried pure tussah silk fibroin in a ternary solution of lithium thiocyanate: mercaptoethanol: urea = 85:5:10 by weight, and treat it for 50 hours at a temperature of 40°C. Dissolved into tussah silk fibroin protein solution, purified by filtration, dialysis, and dried into pure tussah silk fibroin protein;

[0030] 3. Put the pure tussah silk fibroin and polylactic acid with a mass ratio of 90:10 in a formic acid solution with a concentration of 80%, dissolve them for 2 hours at a temperature of 50°C, purify by filtration, and defoam Make the spinning stock solution that the final concentration of solution is 10%;

[0031] 4. Electrospinning is used to spray the above spinning stock solution according to conventional c...

Embodiment 3

[0034] 1, prepare pure tussah silk fibroin by the method for step 1 in embodiment one;

[0035] 2. Treat the dried pure tussah silk fibroin in a solution of lithium thiocyanate: mercaptoethanol: urea = 98:1:1 by weight at a temperature of 80°C for 5 hours, and obtain tussah silkworm after dissolving Silk fibroin solution, filtered, purified, and dried into pure tussah silk fibroin;

[0036] 3. Put pure tussah silk fibroin and gelatin in a mass ratio of 80:20 in hexafluoroisopropanol solution, dissolve at 20°C for 150 hours, filter, purify, and defoam The final concentration of the solution is 5% spinning raw material;

[0037] 4. Using the electrospinning method, the distance from the receiving plate to the spinneret is 15cm, and the voltage is 15KV to spray the above spinning stock solution to obtain a three-dimensional non-woven fabric composed of fibers with a tussah silk fibroin content of 80% and a gelatin content of 20%. Biomedical materials with spun mesh structure. ...

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Abstract

The invention discloses bio-medical engineering material and preparation method thereof, particularly relates to 3D tissue repair material suitable for adhesively growing of cell and preparation method thereof, belonging to bio-medical material technique domain. The natural tussah silk is used as raw material. After antheraea pernyi silk fibroin dissolves, 3D non-woven web structure composed of antheraea pernyi silk fibroin fibre is obtained by electrostatic spinning, wherein the fibre diameter is 50nm-20 mu m, the hole between the fibres is 1-500 mu m, the thickness of non-woven web is 50nm-20mm, and molecular weight of the antheraea pernyi silk fibroin is 10X10<4>-20X10<5>D. The bio-medical engineering material has features of non-poison, harmless, good biocampatibility, good adhesive growth ability of cell, etc. Because of the wide materials supply and low cost, the said material will replace some high-cost bio-medical material such as collagen, and will be used for repair material such as artificial skin, tendon, cartilage, duramater and so on, particularly for histiocyte induction material.

Description

technical field [0001] The invention relates to a biomedical engineering material and a preparation method thereof, in particular to a three-dimensional tissue repair material and a preparation method thereof, which belong to the technical field of biomedical materials. Background technique [0002] Tussah silk contains a large amount of Arg-Gly-Asp (arginine-tyrosine-aspartic acid, RGD) tripeptide sequence, and this sequence has been proved to be beneficial for cell adhesion [Minoura N, Aiba S, Gotoh Y, Tsukada M, Imai Y.Attachment and growth of fibroblast cells on silkfibroin.Biochem Biophys Res Commun, 1995,208(2):511-516; PierschbacherMD, Ruoslahti E, [J].Nature, 1984, (309):30- 33; Pierschbacher MD, Ruoslahti E. Pro Natl Acad Sci USA, 1984, (81): 5985-5988]. This point was proved in the in vitro cell culture experiment of Sofia [Sofia S, McCarthy MB, Gronowic G, et. al. Biomed Mater Res, 2001, (54): 139-148.]. The above disclosed research conte...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/22A61L31/06
Inventor 左保齐
Owner SUZHOU UNIV
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