167 results about "Histiocyte" patented technology

The instant disclosure relates to lipid particles that harbor cationic lipids, the particles found to be capable of delivering associated cargoes - particularly nucleic acid cargoes when formulated as nucleic acid-lipid particles - intracellularly to skin tissue cells when administered topically to a subject. The instant disclosure provides compositions comprising such lipid particles, optionally in association with a therapeutic agent (e.g., a therapeutic mRNA and / or nucleic acid controller system), as well as methods and kits for delivering a lipid particle-associated therapeutic agent and / or for treating or preventing a disease or disorder, e.g., a skin disease or disorder, in a subject, using one or more lipid particle compositions provided herein.

The invention discloses a composition for regulating scalp micro-ecology and application thereof, and relates to the technical field of daily chemical products. The composition for regulating scalp micro-ecology comprises the following components: emu oil, melaleuca alternifolia leaf oil, Brazil green propolis and hydrogenated castor oil. The invention provides a composition for regulating scalp microecology and application thereof, the composition is prepared by compounding an anti-inflammatory and antibacterial component emu oil with melaleuca alternifolia leaf oil, Brazilian green propolis and hydrogenated castor oil, the composition has synergistic bacteriostasis and anti-inflammation effects, keeps scalp microbe balance, reduces red and swollen skin and itching, and also can promote the regeneration capacity of skin tissue cells.

The invention relates to a method for extracting high-purity extracellular vesicles from liver tissues of pelteobagrus vachelli, which comprises the following steps of: firstly, mixing collagenase II and collagenase IV, performing enzymolysis on the tissues, adding a CaCl2 solution, incubating, and digesting the liver tissues into a tissue suspension; then removing cells and fragments through low-speed centrifugation, and filtering to remove impurities by using needle filters of 0.8 [mu] m and 0.22 [mu] m; and finally, further purifying by combining ultracentrifugation with a high-precision iodixanol density gradient centrifugation method, and merging density layers with high purity to obtain high-purity EVs. The method has the characteristics of high extraction purity, high efficiency and simplicity and convenience in operation, and has a wide application prospect.

The invention provides a method, device and system for evaluating the effect of intervening small molecules by tumors and a storage medium, and relates to the technical field of bioinformatics, the method comprises the following steps: obtaining a first transcriptome representation of a tumor cell population before target small molecule intervention and a second transcriptome representation of a normal tissue cell population corresponding to the tumors; inputting the first transcriptome representation and the condition vector into a prediction model to obtain a third transcriptome representation; calculating a first distance and a second distance; an intervention effect is evaluated based on a comparison of the first distance and the second distance. According to the method, the influence of the drug on the transcriptome of the whole cell population is efficiently predicted, and the distance between the transcriptome and the health state is quantitatively compared, so that a set of brand new standard for improving the evaluation target from the traditional'killing cell 'to'reversing disease state' is established, and an objective decision basis is provided for developing more accurate drugs.

The invention provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody as well as a hybridoma cell strain and application thereof, and belongs to the technical field of immune globulin. The invention provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody and also provides a hybridoma cell strain OTI10F11, the hybridoma cell strain OTI10F11 is preserved in the China General Microbiological Culture Collection Center on April 26, 2025, and the preservation number of the hybridoma cell strain OTI10F11 is No.46356. The invention further provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody. The hybridoma cell strain OTI10F11 provided by the invention can stably secrete an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody, and can be specifically combined with H < + > / K < + > ATPase beta protein, so that the specificity, accuracy and reliability of immunodetection are remarkably improved, and the hybridoma cell strain OTI10F11 can be suitable for marking H < + > / K < + > ATPase beta protein in tissue cells.

The invention relates to a sample pretreatment method for desorption electrospray ionization mass spectrometry imaging of a biological tissue frozen section, and the method comprises the following steps: pre-spraying the surface of the frozen biological tissue section, a pre-spraying solvent being a histocompatible solvent, and being used for enhancing the extraction of components in biological tissue cells, the detection sensitivity of components in biological tissues, especially in cells, is improved.

The utility model provides an organ-like tissue breaking device for test tube and sample temperature control accessory thereof, relates to experimental equipment technical field, including protection mechanism, breaking mechanism, locking mechanism and liquid extraction mechanism, breaking mechanism and locking mechanism both are installed in protection mechanism, locking mechanism can push breaking mechanism to move, and the liquid extraction mechanism is located in the protection mechanism. The protection mechanism is arranged at one end of the crushing mechanism, the end of the crushing mechanism extends out of the interior of the protection mechanism and is limited, the liquid taking mechanism is arranged at the other end of the protection mechanism, the end of the liquid taking mechanism is connected with the end of the crushing mechanism in an inserted mode, the crushing mechanism comprises a variable frequency motor, a storage battery, a transmission rod and a crushing tool bit, and the variable frequency motor is electrically connected with the storage battery. During use, the variable frequency motor and the storage battery are respectively arranged in the pen-shaped protective shell to drive the transmission rod to drive the crushing tool bit to rotate to crush tissue cells, and the device is high in integration degree, small in size, capable of directly performing crushing operation in a test tube and capable of crushing a small amount of tissue.

The invention provides a digital breeding-oriented Chinese cabbage leaf cell automatic segmentation and phenotype measurement system and method, and relates to the technical field of plant phenotype analysis. According to the system, joint coding of cell contours and tissue semantics is achieved through a feature extraction sub-network, scale alignment, global feature convergence and feature re-calibration, and pixel-level cell instance masks and tissue categories are output at the same time through mask decoding and category decoding. In combination with multi-tissue phenotypic parameter classification, in-tissue phenotypic index summarization and structured output, cell-level and tissue-level automatic quantitative analysis of upper epidermis, lower epidermis, fence tissues, sponge tissues, vascular bundles and the like is realized, and the efficiency and precision of multi-tissue cell screening and phenotypic statistics are remarkably improved. The method solves the problems that in the prior art, high-precision automatic segmentation and systematic phenotypic measurement for multi-tissue cells of the cross section of the Chinese cabbage leaf are insufficient, and digital breeding decision making is difficult to directly serve.

This invention provides a high-throughput quantitative analysis method and apparatus for biomedical cell and histological staining images. The method includes: image preprocessing, identification of tissue / cell regions, identification of stained regions, and calculation of pixel values ​​of the identified regions to output corresponding quantitative values. This invention can meet the needs of high-throughput, batch processing and analysis of stained image results. It can flexibly adjust the image recognition threshold and selection range through various parameters based on differences in staining background color effects and different background colors generated during image acquisition from different batches of experimental samples, to achieve optimal batch recognition results. Furthermore, this invention can effectively identify and ignore background noise in the image, such as specks similar in color to the target analyte. This invention can automatically process large batches of sample images, saving time and effort, and has greater flexibility in parameter adjustment, generating the required quantitative results based on parameter adjustments.

The invention discloses a composition for enhancing cold resistance based on pyroptosis regulation and a preparation method thereof, and belongs to the technical field of biological medicines. The preparation method of the composition for enhancing cold resistance based on pyroptosis regulation comprises the following steps: S1, respectively preparing an angelica sinensis aqueous extract and an astragalus membranaceus aqueous extract; and S2, mixing the angelica sinensis aqueous extract and the astragalus membranaceus aqueous extract according to the mass ratio of 1: (4-20) to obtain the composition for enhancing cold resistance based on pyroptosis regulation. The invention further provides a composition obtained based on the preparation method, a preparation and application. The composition provided by the invention can significantly prolong the survival time of acute cold exposure mice; the core body temperature of an acute cold exposure mouse is obviously improved, liver injury is relieved, and liver tissue cell pyroptosis pathway protein expression is regulated.

Application of a high-purity inonotus obliquus polysaccharide component belongs to the technical field of biological medicine, and the high-purity inonotus obliquus polysaccharide component plays a role in preventing and treating by inhibiting autophagy of lung tissue cells and depending on an lncRNA Rmst pathway, and can reduce the number of autophagosomes in lung tissues and the level of conversion from LC3-I to LC3-II; according to the method, the sugar content of IOP can be remarkably increased compared with crude polysaccharide, the protein content is remarkably reduced, four active components with specific molecular weights are obtained through separation, the molecular weight ranges are definite (IOP-g1gt, IOP-g2gt, IOP-g3gt and IOP-g4), and the requirements of medicine research and development for controllable purity and definite components are met.

The invention discloses a culture method of in-vitro uniform monolayer cells of prawns, and belongs to the technical field of biology. The method comprises the following steps: (1) obtaining lymphatic tissues of a last-stage prawn at the early stage of shelling, completely stripping an outer membrane to obtain internal tissues, and cutting the internal tissues into small tissue blocks; (2) cleaning the small tissue blocks, inoculating the small tissue blocks to a culture plate coated with a gelatin solution, adding a complete culture solution into an inoculation system at intervals within 12 hours after inoculation for culture, replacing the complete culture solution 24-48 hours after inoculation, and inducing tissue cells to continuously migrate to form an in-vitro cell layer; (3) blowing and beating the cell culture to obtain a cell suspension, and centrifuging to obtain a cell monolayer; and (4) suspending the cell monolayer by adopting a complete culture solution, then inoculating the cell monolayer into a culture plate coated with a gelatin solution, and culturing to obtain the in-vitro uniform monolayer cells of the prawns. According to the method, the prawn monolayer cells which are long in in-vitro survival time, uniform and stable and are cultured in vitro can be obtained, and an important tool is provided for related virology research.

The application discloses an albumin nano-drug based on HSP90 inhibitor and a preparation method and application thereof, and belongs to the technical field of medicines.The short peptide A6 is modified to human blood albumin through a connecting chain, and the human blood albumin modified by the A6 is self-assembled with geldanamycin derivative G2111 in the presence of an organic solvent, so that the G2111 is combined to the hydrophobic region of the human blood albumin through a non-covalent bond, effective loading of the G2111 is realized, the solubility of the geldanamycin derivative is improved, the drug is targetedly released into cancer cells with high expression of CD44, the targeted accumulation of the drug in tumor tissues is enhanced, damage to other normal tissues and cells caused by off-target effects of the drug is avoided, the albumin nano-drug can be simultaneously used for chemotherapy of leukemia and solid tumors, and has important practical significance.

The application relates to the technical field of biological sample pretreatment, and discloses a high-efficiency separation method of a tissue cell extraction device. The method comprises the following steps: performing parameter calibration and reference loss modeling of a driving motor, and eliminating background interference of an actuator; applying a disturbance signal to a dissociation chamber, locking the characteristic resonance frequency of a tissue sample through frequency domain energy analysis, and performing pulsating crushing to accelerate the dissociation of a matrix structure; extracting the net torque of the motor in real time, deducing the fluid dynamic viscosity, adaptively adjusting the motor speed according to the viscosity drop trend, maintaining the fluid shear force in a preset target interval, and avoiding mechanical damage to cells; calculating the mechanical energy dissipation power in real time, and performing feedforward temperature control compensation to eliminate temperature overshoot caused by thermal conduction lag. The application realizes closed-loop control of the physical phase evolution of a heterogeneous tissue dissociation process, solves the limitation of a fixed parameter in a traditional method, shortens the dissociation time, and significantly improves the single-cell yield and survival rate.

The present disclosure provides compositions and methods for the treatment and prevention of macrophage activation syndrome (MAS) or hemophagic lymphocyte hyperplasia (HLH). The methods involve administering to a patient in need thereof an antibody or fragment thereof that is specific for human GM-CSF protein.

The present invention provides lipid nanoparticles for delivering nucleic acid to spleen tissue that can improve the efficiency of nucleic acid delivery to spleen tissue cells, and a method for delivering nucleic acid to spleen tissue by using same. A lipid nanoparticle for use in delivering a nucleic acid to a spleen tissue, including(A) an ionic lipid represented by the formula (1),(B) an anionic phospholipid or a compound represented by the formula (2),(C) cholesterol, and(D) a dimyristoylglycerol PEG represented by the formula:(definitions of symbols in the formula are as described in the specification), and a method for delivering a nucleic acid to a spleen tissue by using same.

The invention relates to a polypeptide oligonucleotide conjugate with a structure as shown in a formula (I) or (II) or a pharmaceutically acceptable salt or a stereoisomer thereof, and an application of the polypeptide oligonucleotide conjugate or the pharmaceutically acceptable salt or the stereoisomer thereof. The polypeptide oligonucleotide conjugate can be used for inhibiting expression of one or more target genes of central nervous system tissue cells, and has the advantages of good in-vivo effect, long action time and good safety.

The invention discloses a pelteobagrus fulvidraco brain tissue cell line PYCB sensitive to viruses and application thereof, and belongs to the technical field of aquatic organism cell culture and disease prevention and control. The preservation number of the Pelteobagrus fulvidraco brain tissue cell line PYCB is CCTCC (China Center For Type Culture Collection) NO: C2025148. The Pelteobagrus fulvidraco brain tissue cell line PYCB is successfully constructed by taking the Pelteobagrus fulvidraco brain tissue as an object, and the cell line can be continuously and stably subcultured, is high in proliferation speed and simple in culture method, is sensitive to Pelteobagrus fulvidraco small RNA viruses and Pelteobagrus fulvidraco stem casing viruses, and can be used for isolated culture of viruses. The invention provides an important research platform and an experimental material for separating and identifying fish viruses and virus vaccines and developing medicines.

To provide a humanized rodent that expresses a heavy chain containing a VL domain. [Solution] A non-human animal, tissue, cell, and genetic material is provided which includes a modification of an endogenous non-human heavy chain immunoglobulin sequence and contains functional ADAM6 activity in a rodent (e.g., mouse), wherein the non-human animal rearranges the human immunoglobulin light chain gene segment in relation to the heavy chain constant domain and expresses an immunoglobulin-like molecule which contains a human immunoglobulin light chain variable domain fused to the heavy chain constant domain and is a cognitive of a human immunoglobulin light chain variable domain fused to the light chain constant domain.

The application provides a method for separating nucleic acid binding proteins in solid tissue cells, and the method comprises the following steps: step S1, preparing solid tissue into a cell suspension 1; step S2, cross-linking the cell suspension 1 by using a cross-linking agent to form a cell suspension 2 with nucleic acid-NABPs complexes; step S3, lysing red blood cells in the cell suspension 2 of step S2 to remove high-abundance protein interference; step S4, after the cells treated in step S3 are lysed by using an organic reagent, the nucleic acid-NABPs complexes in the solid tissue cells are selectively enriched by using affinity chromatography; and step S5, after the nucleic acid-NABPs complexes obtained in step S4 are de-cross-linked, nucleic acid binding proteins (NABPs) in the solid tissue cells are obtained.

The invention relates to an instrument for selecting a small number of cells in tissues at different positions of a space in the technical field of cell culture. The instrument comprises a shell, a fixing device, a cutting station, an operation table, an XYZ-axis moving assembly, a manipulator and a cutting blade, the fixing device is installed on the bottom face of the inner side of the shell, the cutting station is fixed to the fixing device, the XYZ-axis moving assembly is installed on the upper end face of the inner side of the shell, and the XYZ-axis moving assembly receives an instruction of the operation table and moves the mechanical arm to a designated station; the manipulator is installed on the XYZ-axis moving assembly, and the cutting blade is arranged at the end of the manipulator. Cells are selected at different positions in the same space through the XYZ-axis moving assembly, meanwhile, various problems caused by manual operation errors are well avoided through the full-automatic cutting process, the cell selection process is simple and convenient, meanwhile, the mechanical arm is detachably connected with the cutting blade, and the cutting efficiency is improved. An operator can replace cutting blades with different specifications according to actual requirements, so that tissue cells with different sizes can be cut.

The present application relates to the technical field of feeding of crucian carp, and discloses a compound functional feed for repairing carbonate alkali damage of crucian carp, which is composed of alpha-ketoglutaric acid, linoleic acid, laurylene and basic feed, wherein the weight ratio of the alpha-ketoglutaric acid accounts for 1.5% of the total weight of the compound functional feed for repairing carbonate alkali damage of crucian carp, and the weight ratio of the linoleic acid accounts for 0.7% of the total weight of the compound functional feed for repairing carbonate alkali damage of crucian carp. The compound functional feed for repairing carbonate alkali damage of crucian carp can significantly enhance the repair capacity of the tissue cells of the crucian carp, improve the disease resistance of the crucian carp, reduce the risk of diseases and promote the energy metabolism process of the crucian carp, so as to ensure the efficient use of energy and maintain the normal homeostasis of the body.

Methods and compositions are provided to identify oligonucleotides that bind targets of interest. The targets include tissues, cells, circulating biomarkers such as microvesicles, including those derived from various diseases. The oligonucleotides can be used in diagnostic and therapeutic applications.

The application discloses application of a killer cell lectin-like receptor G1 (KLRG1) gene as a marker in preparation of a systemic lupus erythematosus (SLE) secondary hemophagocytic lymphohistiocytosis (HLH) detection preparation, and the preparation is used for detecting the expression level of the KLRG1 gene in a biological sample. Research finds that the expression level of the KLRG1 gene in SLE secondary HLH patients is significantly lower than that of healthy control groups and SLE patients, and has good diagnostic sensitivity and specificity. The application also discloses a preparation for detecting the expression level of the KLRG1 gene in a biological sample of a patient, the preparation contains a primer pair for detecting the mRNA level of the KLRG1 gene expression, or contains an antibody for detecting the protein level of the KLRG1 gene expression, and only needs to extract peripheral blood mononuclear cells (PBMC) after blood drawing to detect whether SLE patients are secondary to HLH, and the preparation is simple, rapid, and accurate in result.

This invention belongs to the field of biology, specifically relating to a composition and its use against foot-and-mouth disease virus (FMDV). This invention discovered that compounds Pasireotide and Oxyclozanide alone cannot significantly inhibit FMDV replication. Unexpectedly, the combined use of Pasireotide and Oxyclozanide significantly inhibits the expression of FMDV RNA polymerase 3D protein, reducing the half-maximal tissue-cell infection dose (TCID) of FMDV. 50 It can significantly reduce the replication ability of foot-and-mouth disease virus and has the effect of antagonizing foot-and-mouth disease virus infection; and there are currently no clear clinical studies or drug instructions mentioning that there is a direct physical / chemical incompatibility between the two, so it can be used to prepare drugs against foot-and-mouth disease virus and has good application prospects.

This invention discloses a method for genetic transformation of bamboo using tRNA-like sequences to assist the cross-species movement of exogenous genes. The method includes the following steps: transferring a target gene from tobacco callus cells to bamboo callus cells using tRNA-like sequences, thereby enabling the target gene to be expressed in the bamboo callus cells; the tRNA-like sequence is shown in SEQ ID No. 1 or SEQ ID No. 2. The method provided by this invention effectively avoids the problem of damage to bamboo callus tissue caused by Agrobacterium infection, providing a new approach for rapid verification of gene function in bamboo in the future.

The application discloses a kind of detection methods and purposes of SOS1 protein content, belong to biological medicine technical field.The method includes the following steps: sample to be tested enzymolysis, obtain the enzymolysis product comprising the characteristic peptide segment of SOS1 protein, the amino acid sequence of characteristic peptide segment is: FEIPEPEPTEADR and QLTLLESDLYR, stable isotope-labeled SOS1 protein characteristic peptide segment is used as internal standard, the signal value of characteristic peptide segment and internal standard peptide segment is detected using liquid chromatography-tandem mass spectrometry, the relative content of SOS1 protein is calculated by peak area ratio, and the absolute content is obtained by substituting calibration curve linear equation.The method can be used for the quantitative detection of SOS1 protein in tumor sample and other tissues, cells, blood sample, with the advantages of high sensitivity, good accuracy and the like.

This invention describes compositions and methods for the delivery of fully human post-translationally modified therapeutic monoclonal antibodies, or their antigen-binding fragments, that bind to C5, to human subjects for ocular indications, particularly for the treatment of AMD. The nucleotide sequence encoding the antibody is delivered via an rAAV vector that targets ocular tissue cells for transgene expression.

The invention relates to the technical field of immune globulin, in particular to an anti-gastrin antibody and a hybridoma cell strain and application thereof. The invention provides an anti-gastrin antibody, and also provides a hybridoma cell strain OTI3D11, and the preservation number of the hybridoma cell strain is CGMCC (China General Microbiological Culture Collection Center) No.46359. The invention also provides an anti-gastrin antibody and a hybridoma cell strain OTI3D11. According to the present invention, the hybridoma cell strain OTI3D11 can stably secrete the anti-gastrin monoclonal antibody, and can specifically bind to the Gastrin protein (especially G-17), such that the immunodetection of the hybridoma cell strain OTI3D11 has specificity and sensitivity, and the hybridoma cell strain OTI3D11 can be suitable for the labeling of the Gastrin protein in tissue cells.