506 results about "Histiocyte" patented technology

The invention discloses a drug resistance prediction method and system based on comparative learning and multi-modal fusion, and the method comprises the steps: firstly generating a molecular map and a molecular fingerprint based on the SMILES of a target drug, and extracting the molecular features of the drug through a comparative learning model constructed through combining a map attention network and a map convolution network; and then, acquiring protein expression, gene expression and metabolic expression data from the target tissue cells, extracting modal features through a deep convolutional network, a Transform encoder and a multi-dimensional attention network, and realizing adaptive fusion of the multi-modal features through a heterogeneous interactive attention mechanism. And finally, jointly inputting the fused multi-modal features and drug molecular features into a multi-layer sensor to realize high-precision prediction of the drug resistance of cells to drugs. By introducing a contrast learning and multi-modal feature fusion mechanism, the characterization capability and prediction precision of the model are effectively improved, and efficient and reliable support can be provided for drug screening and clinical decision making.

The invention belongs to the technical field of kidney histopathology and immunological evaluation, and particularly relates to a kidney pathology detection kit, a detection method and application, the detection kit comprises 37 rare metal element labeled monoclonal antibodies combined with antigens of in-situ tissue cells, and the detection method comprises the following steps: (1) collecting a sample to be detected, preparing a pre-dyed paraffin section; (2) mixing 37 monoclonal antibodies labeled by rare metal elements, and carrying out antibody staining on various antigen targets on the surfaces of cells in the in-situ tissue; (3) carrying out nucleus DNA staining on the stained tissue sample; and (4) detecting the stained tissue sample through an imaging mass spectrum flow system, and collecting original information data through laser spot firing. The method is an analysis method for fine grouping identification and relative quantification of structural cells, immune cells and pathological injury cells in the clinical nephropathy sample in-situ tissue. And auxiliary diagnosis information is provided for diagnosis, grading, treatment and prognosis detection of various clinical nephropathy.

The present invention relates to the field of tissue-derived cell identification, in particular to a method for identifying tissue-derived cells in the body fluid based on single-cell sequencing technology. In the present invention, on the basis of high-throughput single-cell RNA sequencing data of cell samples obtained from body fluids, by means of reference component analysis (RCA), expression of known tissue-related marker genes, and a tissue-derived cell prediction model constructed based on logistic regression, the specific identification of tissue-derived cells in body fluids is achieved.

The invention provides a mammary gland pathological cell image detection method and system based on an improved YOLOv7 model, and the method comprises the steps: S1, collecting mammary gland pathological section images, and generating a data set; s2, carrying out frame labeling on cells obtained in the data set; s3, segmenting the marked data set into a training set and a verification set; step S4, establishing a YOLOv7 network, and respectively adding an ASFF module and a CBAM module in three branches of the YOLOv7 network to obtain an improved YOLOv7 network; and S5, inputting the training set into the improved YOLOv7 network for training, and verifying to obtain the high-precision breast pathological section cell detection model. The improved model can effectively avoid a large semantic gap between non-adjacent levels of mammary gland cell characteristics, greatly enhance the detection capability of multi-scale cell targets such as tiny early cancer cells and large normal mammary gland tissue cells, and improve the recognition precision of early mammary gland lesion cells.

The instant disclosure relates to lipid particles that harbor cationic lipids, the particles found to be capable of delivering associated cargoes - particularly nucleic acid cargoes when formulated as nucleic acid-lipid particles - intracellularly to skin tissue cells when administered topically to a subject. The instant disclosure provides compositions comprising such lipid particles, optionally in association with a therapeutic agent (e.g., a therapeutic mRNA and / or nucleic acid controller system), as well as methods and kits for delivering a lipid particle-associated therapeutic agent and / or for treating or preventing a disease or disorder, e.g., a skin disease or disorder, in a subject, using one or more lipid particle compositions provided herein.

The invention provides a tissue protection solution and application thereof. The tissue protection liquid comprises a basic culture medium; vitamins, wherein the vitamins comprise vitamin E; the antioxidant is prepared from at least one of N-acetylcysteine amide, superoxide dismutase and catalase; and a specific cell factor, wherein the specific cell factor comprises at least one of a ROCK inhibitor Y27632 and insulin. Therefore, when the tissue protection liquid is used for storing and transporting a tissue sample, the loss in the process from tissue sample separation to experiment starting can be reduced, the function and activity of tissue cells are ensured, and the success rate of establishment of various primary tissue cultures is effectively improved.

The invention discloses an application of a Piezo1 inhibitor in preparation of a medicine for treating necrotizing enterocolitis. The Piezo1 inhibitor can be applied in preparation of the medicine for treating the necrotizing enterocolitis. The Piezo1 inhibitor can also be applied to preparation of drugs for relieving NEC intestinal epithelial barrier injury. The Piezo1 inhibitor can also be applied to preparation of drugs for reducing calcium influx in intestinal epithelial cells and calcium / calmodulin dependent protein kinase activity. The application has the beneficial effects that the effect and mechanism of the Piezo1 for regulating and controlling the calcium ion influx and promoting the NEC severity degree by the calcium / calmodulin dependent protein kinase activity level are discussed for the first time from multiple aspects of animals, tissues, cells, molecules and the like, and the effect and mechanism of the activated intestinal epithelial cell Piezo1 in NEC inflammatory injury and barrier repair are clarified for the first time; the knockout of the gene can improve the NEC severity of mice, and a new intervention target is provided for clinical treatment.

The invention discloses a composition for regulating scalp micro-ecology and application thereof, and relates to the technical field of daily chemical products. The composition for regulating scalp micro-ecology comprises the following components: emu oil, melaleuca alternifolia leaf oil, Brazil green propolis and hydrogenated castor oil. The invention provides a composition for regulating scalp microecology and application thereof, the composition is prepared by compounding an anti-inflammatory and antibacterial component emu oil with melaleuca alternifolia leaf oil, Brazilian green propolis and hydrogenated castor oil, the composition has synergistic bacteriostasis and anti-inflammation effects, keeps scalp microbe balance, reduces red and swollen skin and itching, and also can promote the regeneration capacity of skin tissue cells.

The invention belongs to the technical field of biomedical engineering and microfluidics, and particularly discloses a vascularized organ chip and method for large-scale culture, the vascularized organ chip comprises an upper runner layer and a lower culture layer, and the runner layer and the culture layer are fixedly sealed; a liquid storage pipe matched with the flow channel layer is adhered to the upper surface of the flow channel layer; according to the vascularized organ chip, the flow channel layer is used for pouring a culture solution to provide fluid stimulation and nutrients for cells; the three parallel-connected large-scale culture chambers of the culture layer are used for culturing cells, so that blood circulation of a microvascular system in a human body can be simulated, and a microenvironment in tissues can be reproduced more truly; meanwhile, the requirement for culturing a large number of tissue cells through the chip can be met, and the cell yield and the experiment scale can be effectively increased.

The invention discloses an AAV vector targeting improvement method applicable to gene therapy drugs, which comprises the following steps: performing high-throughput analysis on target tissue cells through a screening sub-module, screening candidate receptor genes interacting with AAV vector targeting molecules, and determining target receptor genes; the selection submodule is used for designing a gene editing system and selecting a promoter with tissue specific expression to drive the expression of the gene editing system; the introduction submodule is used for introducing the gene editing system into target tissue cells by adopting a gene transfer technology; and the evaluation sub-module is used for selecting the most suitable gene transfer mode according to the characteristics and treatment requirements of the target tissue, and detecting and evaluating the cells subjected to gene editing by utilizing a PCR molecular biological technology. The targeting property of the AAV vector to target tissue cells is improved, the infection of non-target tissues is reduced, and the side effect risk is reduced, so that the gene therapy effect is remarkably improved.

The invention discloses a schizophrenia risk structure variation identification and function evaluation method based on three-generation sequencing, and relates to the field of molecular biology, whole genome sequencing is performed on peripheral blood DNA of a patient through three-generation sequencing, multi-tool joint detection is adopted, multi-sample results are integrated, and a high-confidence SV data set is generated; through cross-queue comparison, the patient specific SV is screened, and the high-risk potential pathogenic SV is identified in combination with an SV priority ordering tool SVJudge. By combining transcription factor binding analysis, SCZ drug target data and histocyte specific expression data, the influence of SV on gene regulation is evaluated, and the potential action mechanism of SV in SCZ is analyzed. The SCZ risk gene is screened based on SVJudge scoring and patient carrying conditions, the genetic risk and pathogenic mechanism of the SCZ are analyzed and verified through pathway enrichment, a protein interaction network and a functional module, and a new technical means and theoretical basis are provided for genetic research of the SCZ.

The method comprises the following steps: fixing with a pre-fixing solution, wrapping and forming with a solid agent, fixing with a fixing solution, embedding with an embedding agent, decomposing melanin with a melanin decomposing solution, pre-decoloring with a pre-decoloring solution, decoloring with a decoloring solution, and eluting with a rinsing solution. The invention provides a melanin removing method capable of effectively avoiding retina RPE layer cell exfoliation, protein denaturation and nucleic acid degradation. The method mainly comprises the following steps: keeping the original state of tissue cells by adopting a pre-fixing solution, keeping the complete tissue structure by adopting a solid agent, decomposing melanin particles by adopting a melanin decomposing solution composed of various extracts, separating melanin by adopting a pre-decoloring solution and a decoloring solution, eluting the melanin decomposed on the surface of the tissue by adopting a rinsing solution, and the like. On the premise that the retina tissue structure is intact and protein, nucleic acid and cell structures on the surface of the tissue section are not damaged, melanin particles in the tissue section are effectively decomposed, the biological activity of various organelles on the surface of the section is kept, and the requirements of subsequent chemical staining of various tissues are met.

The invention relates to the technical field of tissue engineering, and particularly discloses preparation of an exosome and electrostatic spinning composite material for promoting bone regeneration and application of the exosome and electrostatic spinning composite material in bone tissue regeneration. Identifying the multiplication capacity, the surface marker and the multidirectional differentiation capacity of the cells after the cells are separated and cultured, and finally extracting and identifying the exosome; pLLA and gelatin are dissolved in a cosolvent trifluoroethanol, composite fibers are prepared through electrostatic spinning, and a composite fiber membrane is obtained through spinning by adopting a receiver; the exosome is loaded on the surface of the PLLA / gelatin composite fiber membrane. According to the invention, the exosome derived from human mesenchymal stem cells is combined with the membrane material to prepare the exosome capable of stably releasing, the good stability and low immunogenicity of the exosome are exerted, and the effect of accurately promoting local bone regeneration is realized. And defect repair and functional reconstruction are realized.

The invention discloses an extraction method and application of high-molecular-weight turbellarian worm genome DNA, and belongs to the field of turbellarian worm genome extraction.The turbellarian worm genome DNA extraction is achieved by improving a lysate formula and removing ethylenediaminetetraacetic acid and increasing Mg < 2 + > on the basis of a conventional reagent in a classic Tris-HCl-phenol-chloroform method to inhibit activity of deoxyribonuclease II in a turbellarian worm body to inhibit degradation of genome DNA, and the high-molecular-weight turbellarian worm genome DNA is obtained. Meanwhile, ribonuclease A is added, RNA in cells can be degraded in the tissue cell digestion process, so that the step of two times of purification is omitted, the loss of DNA yield is reduced, experimental steps are simplified, especially the step of DNA purification is reduced, the problem of degradation in the extraction process of the turbellarian turbellarian worm genome DNA is solved, and the genome DNA yield (gt; 10 micrograms per turbellarian worm), and the turbellarian worm genome ultra-long sequencing or PacBio high-fidelity sequencing requirements are met.

The utility model discloses a thyroid needle biopsy device, including thyroid needle biopsy device main part, first locking piece, second locking piece and cushion pad, the inner wall of alcohol tube is movably connected with push component, the bottom of spray tube inner wall is fixedly connected with dispersion component. Through the arrangement of the mounting plate, the alcohol pipe and the spray pipe, the alcohol pipe can be fixed and limited by the mounting plate, the alcohol pipe is used for storing disinfection alcohol, and the disinfection alcohol can be sprayed to the skin of a patient through the spray pipe, so that the problem that the skin of the patient needs to be disinfected by alcohol before thyroid tissue cells are obtained is solved; however, the problems that an existing thyroid needle biopsy device body does not have a disinfection function, an alcohol disinfection device needs to be additionally prepared in advance, great inconvenience is caused, and the efficiency and the quality of cell extraction are affected due to the fact that the outer skin of a cell extraction area is not accurately disinfected manually are solved.

The present invention provides a method for preparing a keratinized gingival repair scaffold, wherein the active powder of biological microspheres is dispersed in a protein slurry to obtain a loose surface stock solution, which is freeze-dried together with a cell-free matrix material, and after two cross-linkings, a keratinized gingival repair scaffold with a loose surface and a dense surface is obtained. When in use, the loose surface of the porous sponge structure contacts the wound surface, which is conducive to the attachment and growth of tissue cells, and the dense surface can seal and protect the wound to a certain extent in an open healing environment. The obtained keratinized gingival repair scaffold can effectively maintain and regulate the pH environment in the oral cavity, inhibit bacteria and resist bacteria, and inhibit the growth of acid-producing bacteria such as Streptococcus mutans and bacteria with strong caries-causing properties, and at the same time has the ability to stop bleeding, promote bone growth and periodontal ligament regeneration, and inhibit periodontal epithelial regression.

The invention relates to a culture method of a pancreatic cancer organ model. The culture method comprises the following steps: cleaning tissues and chopping the tissues through a chopping device; transferring the chopped tissue into a centrifugal tube filled with tissue digestive juice for digestion, and collecting upper suspension and residual tissue precipitate; mixing the collected upper-layer suspension with the digested residual precipitate, and treating to obtain cell and tissue precipitates; embedding the tissues and the cells into matrigel; and after the matrigel is solidified, culturing tissue cells by using a corresponding organoid culture medium, and obtaining primary organoid tissues which are pancreatic cancer tissues. Due to the adoption of the technical scheme, the culture method of the pancreatic cancer organoid model can be used for stably culturing pancreatic cancer organoid with high success rate, and the invention also relates to a culture method of the pancreatic cancer para-carcinoma organoid model.

The present disclosure provides a method and a gene detection panel for evaluating treatment response, recurrence and survival by detecting genetic variants and their changes before and after concurrent chemoradiotherapy in tumor tissues of patients with esophageal cancer. The present disclosure develops a set of esophageal cancer NGS analysis panel. Aiming at 402 mutation sites including 35 genes that frequently occur in esophageal squamous cell carcinoma tissue cells, 62 pairs of esophageal squamous cell carcinoma tissues before and after CCRT are analyzed for specific site variation, hoping to find new predictive markers. The present disclosure combines these potential markers into an esophageal cancer detection panel, which has extremely high value for improving the prognosis of esophageal cancer.

The present invention belongs to the field of traditional Chinese medicines, and particularly relates to Leixihaokang, a preparation method therefor, and use thereof. The Leixihaokang of the present invention is prepared from the following active pharmaceutical ingredients in parts by weight: 10 parts of Tripterygium glycosides and 0.1-1.3 parts of Artemisia annua extract. According to the present invention, animal experiments prove that the Leixihaokang can ameliorate renal damage in nephrotic syndrome caused by adriamycin, reduce the occurrence of proteinuria, and reduce the apoptosis of renal tissue cells. Meanwhile, the Leixihaokang of the present invention has a good inhibitory effect on renal, hepatic, splenic, and cardiac toxicity in the treatment process of nephrotic syndrome. According to the present invention, animal experiments also prove that the Leixihaokang can ameliorate skin injury in imiquimod-induced psoriasis, reduce inflammatory infiltration and alleviate skin damage. Meanwhile, the Leixihaokang has a certain degree of inhibitory effect on the hepatic and reproductive system toxicity in the treatment process of psoriasis.

The invention relates to a method for extracting high-purity extracellular vesicles from liver tissues of pelteobagrus vachelli, which comprises the following steps of: firstly, mixing collagenase II and collagenase IV, performing enzymolysis on the tissues, adding a CaCl2 solution, incubating, and digesting the liver tissues into a tissue suspension; then removing cells and fragments through low-speed centrifugation, and filtering to remove impurities by using needle filters of 0.8 [mu] m and 0.22 [mu] m; and finally, further purifying by combining ultracentrifugation with a high-precision iodixanol density gradient centrifugation method, and merging density layers with high purity to obtain high-purity EVs. The method has the characteristics of high extraction purity, high efficiency and simplicity and convenience in operation, and has a wide application prospect.

The invention discloses a multi-immunohistocyte image segmentation method, and relates to the technical field of image processing, and the method comprises the steps: obtaining a to-be-segmented multi-immunohistocyte image, and carrying out the image focusing processing of the multi-immunohistocyte image, so as to obtain a target multi-immunohistocyte image containing a cell internal region and a background region; performing uniform sampling processing on the target multi-immune tissue cell image to form a prompt point set; carrying out mapping processing on the prompt point set through a pre-training cell image segmentation model, and carrying out feature extraction on the multi-immune tissue cell image to obtain a prompt embedding vector and an image embedding vector; and performing mask processing of global semantic features and local detail features on the prompt embedding vector and the image embedding vector, generating a segmentation mask corresponding to each prompt point, integrating the segmentation masks corresponding to the prompt points, and outputting a cell image segmentation result of the multiple immune tissue cell images. And accurate identification of the multi-scale cells is realized.

The invention provides a method, device and system for evaluating the effect of intervening small molecules by tumors and a storage medium, and relates to the technical field of bioinformatics, the method comprises the following steps: obtaining a first transcriptome representation of a tumor cell population before target small molecule intervention and a second transcriptome representation of a normal tissue cell population corresponding to the tumors; inputting the first transcriptome representation and the condition vector into a prediction model to obtain a third transcriptome representation; calculating a first distance and a second distance; an intervention effect is evaluated based on a comparison of the first distance and the second distance. According to the method, the influence of the drug on the transcriptome of the whole cell population is efficiently predicted, and the distance between the transcriptome and the health state is quantitatively compared, so that a set of brand new standard for improving the evaluation target from the traditional'killing cell 'to'reversing disease state' is established, and an objective decision basis is provided for developing more accurate drugs.

The invention relates to the field of biological medicine, in particular to application of S100A9 in treatment or detection of diabetic cardiomyopathy. The research finds that the expression of S100A9 in the heart tissue of a diabetic cardiomyopathy mouse is obviously increased. Overexpression of the S100A9 can aggravate myocardial injury and cardiac dysfunction, and inhibition of expression or activity of the S100A9 can improve illness conditions. Both the S100A9 inhibitor paquinimod and the macrophage scavenger chlorophosphonate liposome can reduce the concentration of S100A9 in serum, relieve cardiac enlargement and myocardial fibrosis and recover the left ventricular ejection fraction. In addition, the S100A9 can be used as a marker for detecting the diabetic cardiomyopathy, and related detection products can reflect the severity of the illness state by detecting the expression quantity of the S100A9 in tissue cells or body fluid. The invention provides a new target spot, medicine and detection means for treatment and detection of diabetic cardiomyopathy.

The tissue repair preparation at least comprises ECM, exosome and degradable glue, the ECM is an acellular matrix such as SIS, SIM and UBM, is granular and is injectable micro powder, and the content of the ECM is 10-50 mg / ml; the exosome is a healing-promoting cell, and is obtained by induction, polarization culture, centrifugation, supernatant collection and concentration and purification. The degradable glue is a polysaccharide with viscoelasticity; the advantages of a conventional filling agent are reserved, exosomes can be slowly released and internalized by various tissue cells, the activity of fibroblasts can be stimulated, the effect is lasting, and generation of new collagen is promoted; the defects of short effect and non-lasting effect of the existing filling agent are overcome; the product provided by the invention can achieve the effects of promoting rapid regeneration of collagen and maintaining for a long time; the functions of filling, face-lifting, tissue repairing and the like are achieved; the effect is remarkable, stable and lasting; the composition has multiple functions of quick effect, obvious effect and slow release and long-acting effect.

The invention provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody as well as a hybridoma cell strain and application thereof, and belongs to the technical field of immune globulin. The invention provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody and also provides a hybridoma cell strain OTI10F11, the hybridoma cell strain OTI10F11 is preserved in the China General Microbiological Culture Collection Center on April 26, 2025, and the preservation number of the hybridoma cell strain OTI10F11 is No.46356. The invention further provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody. The hybridoma cell strain OTI10F11 provided by the invention can stably secrete an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody, and can be specifically combined with H < + > / K < + > ATPase beta protein, so that the specificity, accuracy and reliability of immunodetection are remarkably improved, and the hybridoma cell strain OTI10F11 can be suitable for marking H < + > / K < + > ATPase beta protein in tissue cells.

The invention discloses a morphological prediction method for tissue cells and blood cells of a fluorescence in-situ hybridization image, relates to the field of data image processing, and solves the common problems of fuzzy cell boundary, heterogeneity cell morphological difference, high noise background, insufficient data labeling, low efficiency, subjectivity and the like in the existing fluorescence in-situ hybridization image analysis. The method comprises the following steps: acquiring tissue cells and blood cells of a fluorescence in-situ hybridization image, and performing multi-scale image enhancement on the tissue cells and the blood cells; extracting local maximum values and local minimum values of tissue cells and blood cells by adopting a local extreme value feature extraction method; identifying the morphological characteristics of the edge of the cell nucleus for confirming the cell nucleus morphology of the convex defect of the cell nucleus; and based on the morphological characteristics of the identified cell nucleus edge and the morphological characteristics of the cell nucleus with the convex defect, finishing the prediction of the final morphology of the cell nucleus. The invention is also applicable to the fields of biology and cancer detection.

The invention relates to a sample pretreatment method for desorption electrospray ionization mass spectrometry imaging of a biological tissue frozen section, and the method comprises the following steps: pre-spraying the surface of the frozen biological tissue section, a pre-spraying solvent being a histocompatible solvent, and being used for enhancing the extraction of components in biological tissue cells, the detection sensitivity of components in biological tissues, especially in cells, is improved.

The utility model provides an organ-like tissue breaking device for test tube and sample temperature control accessory thereof, relates to experimental equipment technical field, including protection mechanism, breaking mechanism, locking mechanism and liquid extraction mechanism, breaking mechanism and locking mechanism both are installed in protection mechanism, locking mechanism can push breaking mechanism to move, and the liquid extraction mechanism is located in the protection mechanism. The protection mechanism is arranged at one end of the crushing mechanism, the end of the crushing mechanism extends out of the interior of the protection mechanism and is limited, the liquid taking mechanism is arranged at the other end of the protection mechanism, the end of the liquid taking mechanism is connected with the end of the crushing mechanism in an inserted mode, the crushing mechanism comprises a variable frequency motor, a storage battery, a transmission rod and a crushing tool bit, and the variable frequency motor is electrically connected with the storage battery. During use, the variable frequency motor and the storage battery are respectively arranged in the pen-shaped protective shell to drive the transmission rod to drive the crushing tool bit to rotate to crush tissue cells, and the device is high in integration degree, small in size, capable of directly performing crushing operation in a test tube and capable of crushing a small amount of tissue.

The invention provides a digital breeding-oriented Chinese cabbage leaf cell automatic segmentation and phenotype measurement system and method, and relates to the technical field of plant phenotype analysis. According to the system, joint coding of cell contours and tissue semantics is achieved through a feature extraction sub-network, scale alignment, global feature convergence and feature re-calibration, and pixel-level cell instance masks and tissue categories are output at the same time through mask decoding and category decoding. In combination with multi-tissue phenotypic parameter classification, in-tissue phenotypic index summarization and structured output, cell-level and tissue-level automatic quantitative analysis of upper epidermis, lower epidermis, fence tissues, sponge tissues, vascular bundles and the like is realized, and the efficiency and precision of multi-tissue cell screening and phenotypic statistics are remarkably improved. The method solves the problems that in the prior art, high-precision automatic segmentation and systematic phenotypic measurement for multi-tissue cells of the cross section of the Chinese cabbage leaf are insufficient, and digital breeding decision making is difficult to directly serve.

The invention discloses a method for extracting calreticulin from tissues and application. The tissue cell extract extracted by the method contains calreticulin and can be used for preparing drugs or vaccines for preventing cancers. Experimental results show that after the tissue cell extract is immunized for several weeks, specific immune response of an organism can be triggered, cancerous cells in the organism can be removed, and therefore the aim of preventing cancers is achieved. The tissue cell extract provided by the invention can be used as a tumor preventive vaccine for preventing autologous or allogenic tumors and reducing the incidence rate of cancers in healthy people.