264 results about "Histiocyte" patented technology

The invention discloses a drug resistance prediction method and system based on comparative learning and multi-modal fusion, and the method comprises the steps: firstly generating a molecular map and a molecular fingerprint based on the SMILES of a target drug, and extracting the molecular features of the drug through a comparative learning model constructed through combining a map attention network and a map convolution network; and then, acquiring protein expression, gene expression and metabolic expression data from the target tissue cells, extracting modal features through a deep convolutional network, a Transform encoder and a multi-dimensional attention network, and realizing adaptive fusion of the multi-modal features through a heterogeneous interactive attention mechanism. And finally, jointly inputting the fused multi-modal features and drug molecular features into a multi-layer sensor to realize high-precision prediction of the drug resistance of cells to drugs. By introducing a contrast learning and multi-modal feature fusion mechanism, the characterization capability and prediction precision of the model are effectively improved, and efficient and reliable support can be provided for drug screening and clinical decision making.

The present invention relates to the field of tissue-derived cell identification, in particular to a method for identifying tissue-derived cells in the body fluid based on single-cell sequencing technology. In the present invention, on the basis of high-throughput single-cell RNA sequencing data of cell samples obtained from body fluids, by means of reference component analysis (RCA), expression of known tissue-related marker genes, and a tissue-derived cell prediction model constructed based on logistic regression, the specific identification of tissue-derived cells in body fluids is achieved.

The invention provides a mammary gland pathological cell image detection method and system based on an improved YOLOv7 model, and the method comprises the steps: S1, collecting mammary gland pathological section images, and generating a data set; s2, carrying out frame labeling on cells obtained in the data set; s3, segmenting the marked data set into a training set and a verification set; step S4, establishing a YOLOv7 network, and respectively adding an ASFF module and a CBAM module in three branches of the YOLOv7 network to obtain an improved YOLOv7 network; and S5, inputting the training set into the improved YOLOv7 network for training, and verifying to obtain the high-precision breast pathological section cell detection model. The improved model can effectively avoid a large semantic gap between non-adjacent levels of mammary gland cell characteristics, greatly enhance the detection capability of multi-scale cell targets such as tiny early cancer cells and large normal mammary gland tissue cells, and improve the recognition precision of early mammary gland lesion cells.

The instant disclosure relates to lipid particles that harbor cationic lipids, the particles found to be capable of delivering associated cargoes - particularly nucleic acid cargoes when formulated as nucleic acid-lipid particles - intracellularly to skin tissue cells when administered topically to a subject. The instant disclosure provides compositions comprising such lipid particles, optionally in association with a therapeutic agent (e.g., a therapeutic mRNA and / or nucleic acid controller system), as well as methods and kits for delivering a lipid particle-associated therapeutic agent and / or for treating or preventing a disease or disorder, e.g., a skin disease or disorder, in a subject, using one or more lipid particle compositions provided herein.

The invention discloses a composition for regulating scalp micro-ecology and application thereof, and relates to the technical field of daily chemical products. The composition for regulating scalp micro-ecology comprises the following components: emu oil, melaleuca alternifolia leaf oil, Brazil green propolis and hydrogenated castor oil. The invention provides a composition for regulating scalp microecology and application thereof, the composition is prepared by compounding an anti-inflammatory and antibacterial component emu oil with melaleuca alternifolia leaf oil, Brazilian green propolis and hydrogenated castor oil, the composition has synergistic bacteriostasis and anti-inflammation effects, keeps scalp microbe balance, reduces red and swollen skin and itching, and also can promote the regeneration capacity of skin tissue cells.

The invention relates to a method for extracting high-purity extracellular vesicles from liver tissues of pelteobagrus vachelli, which comprises the following steps of: firstly, mixing collagenase II and collagenase IV, performing enzymolysis on the tissues, adding a CaCl2 solution, incubating, and digesting the liver tissues into a tissue suspension; then removing cells and fragments through low-speed centrifugation, and filtering to remove impurities by using needle filters of 0.8 [mu] m and 0.22 [mu] m; and finally, further purifying by combining ultracentrifugation with a high-precision iodixanol density gradient centrifugation method, and merging density layers with high purity to obtain high-purity EVs. The method has the characteristics of high extraction purity, high efficiency and simplicity and convenience in operation, and has a wide application prospect.

The invention discloses a multi-immunohistocyte image segmentation method, and relates to the technical field of image processing, and the method comprises the steps: obtaining a to-be-segmented multi-immunohistocyte image, and carrying out the image focusing processing of the multi-immunohistocyte image, so as to obtain a target multi-immunohistocyte image containing a cell internal region and a background region; performing uniform sampling processing on the target multi-immune tissue cell image to form a prompt point set; carrying out mapping processing on the prompt point set through a pre-training cell image segmentation model, and carrying out feature extraction on the multi-immune tissue cell image to obtain a prompt embedding vector and an image embedding vector; and performing mask processing of global semantic features and local detail features on the prompt embedding vector and the image embedding vector, generating a segmentation mask corresponding to each prompt point, integrating the segmentation masks corresponding to the prompt points, and outputting a cell image segmentation result of the multiple immune tissue cell images. And accurate identification of the multi-scale cells is realized.

The invention provides a method, device and system for evaluating the effect of intervening small molecules by tumors and a storage medium, and relates to the technical field of bioinformatics, the method comprises the following steps: obtaining a first transcriptome representation of a tumor cell population before target small molecule intervention and a second transcriptome representation of a normal tissue cell population corresponding to the tumors; inputting the first transcriptome representation and the condition vector into a prediction model to obtain a third transcriptome representation; calculating a first distance and a second distance; an intervention effect is evaluated based on a comparison of the first distance and the second distance. According to the method, the influence of the drug on the transcriptome of the whole cell population is efficiently predicted, and the distance between the transcriptome and the health state is quantitatively compared, so that a set of brand new standard for improving the evaluation target from the traditional'killing cell 'to'reversing disease state' is established, and an objective decision basis is provided for developing more accurate drugs.

The invention provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody as well as a hybridoma cell strain and application thereof, and belongs to the technical field of immune globulin. The invention provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody and also provides a hybridoma cell strain OTI10F11, the hybridoma cell strain OTI10F11 is preserved in the China General Microbiological Culture Collection Center on April 26, 2025, and the preservation number of the hybridoma cell strain OTI10F11 is No.46356. The invention further provides an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody. The hybridoma cell strain OTI10F11 provided by the invention can stably secrete an anti-human H < + > / K < + > ATPase beta protein monoclonal antibody, and can be specifically combined with H < + > / K < + > ATPase beta protein, so that the specificity, accuracy and reliability of immunodetection are remarkably improved, and the hybridoma cell strain OTI10F11 can be suitable for marking H < + > / K < + > ATPase beta protein in tissue cells.

The invention relates to a sample pretreatment method for desorption electrospray ionization mass spectrometry imaging of a biological tissue frozen section, and the method comprises the following steps: pre-spraying the surface of the frozen biological tissue section, a pre-spraying solvent being a histocompatible solvent, and being used for enhancing the extraction of components in biological tissue cells, the detection sensitivity of components in biological tissues, especially in cells, is improved.

The utility model provides an organ-like tissue breaking device for test tube and sample temperature control accessory thereof, relates to experimental equipment technical field, including protection mechanism, breaking mechanism, locking mechanism and liquid extraction mechanism, breaking mechanism and locking mechanism both are installed in protection mechanism, locking mechanism can push breaking mechanism to move, and the liquid extraction mechanism is located in the protection mechanism. The protection mechanism is arranged at one end of the crushing mechanism, the end of the crushing mechanism extends out of the interior of the protection mechanism and is limited, the liquid taking mechanism is arranged at the other end of the protection mechanism, the end of the liquid taking mechanism is connected with the end of the crushing mechanism in an inserted mode, the crushing mechanism comprises a variable frequency motor, a storage battery, a transmission rod and a crushing tool bit, and the variable frequency motor is electrically connected with the storage battery. During use, the variable frequency motor and the storage battery are respectively arranged in the pen-shaped protective shell to drive the transmission rod to drive the crushing tool bit to rotate to crush tissue cells, and the device is high in integration degree, small in size, capable of directly performing crushing operation in a test tube and capable of crushing a small amount of tissue.

The invention provides a digital breeding-oriented Chinese cabbage leaf cell automatic segmentation and phenotype measurement system and method, and relates to the technical field of plant phenotype analysis. According to the system, joint coding of cell contours and tissue semantics is achieved through a feature extraction sub-network, scale alignment, global feature convergence and feature re-calibration, and pixel-level cell instance masks and tissue categories are output at the same time through mask decoding and category decoding. In combination with multi-tissue phenotypic parameter classification, in-tissue phenotypic index summarization and structured output, cell-level and tissue-level automatic quantitative analysis of upper epidermis, lower epidermis, fence tissues, sponge tissues, vascular bundles and the like is realized, and the efficiency and precision of multi-tissue cell screening and phenotypic statistics are remarkably improved. The method solves the problems that in the prior art, high-precision automatic segmentation and systematic phenotypic measurement for multi-tissue cells of the cross section of the Chinese cabbage leaf are insufficient, and digital breeding decision making is difficult to directly serve.

The invention discloses a method for extracting calreticulin from tissues and application. The tissue cell extract extracted by the method contains calreticulin and can be used for preparing drugs or vaccines for preventing cancers. Experimental results show that after the tissue cell extract is immunized for several weeks, specific immune response of an organism can be triggered, cancerous cells in the organism can be removed, and therefore the aim of preventing cancers is achieved. The tissue cell extract provided by the invention can be used as a tumor preventive vaccine for preventing autologous or allogenic tumors and reducing the incidence rate of cancers in healthy people.

This invention provides a high-throughput quantitative analysis method and apparatus for biomedical cell and histological staining images. The method includes: image preprocessing, identification of tissue / cell regions, identification of stained regions, and calculation of pixel values ​​of the identified regions to output corresponding quantitative values. This invention can meet the needs of high-throughput, batch processing and analysis of stained image results. It can flexibly adjust the image recognition threshold and selection range through various parameters based on differences in staining background color effects and different background colors generated during image acquisition from different batches of experimental samples, to achieve optimal batch recognition results. Furthermore, this invention can effectively identify and ignore background noise in the image, such as specks similar in color to the target analyte. This invention can automatically process large batches of sample images, saving time and effort, and has greater flexibility in parameter adjustment, generating the required quantitative results based on parameter adjustments.

The invention discloses a composition for enhancing cold resistance based on pyroptosis regulation and a preparation method thereof, and belongs to the technical field of biological medicines. The preparation method of the composition for enhancing cold resistance based on pyroptosis regulation comprises the following steps: S1, respectively preparing an angelica sinensis aqueous extract and an astragalus membranaceus aqueous extract; and S2, mixing the angelica sinensis aqueous extract and the astragalus membranaceus aqueous extract according to the mass ratio of 1: (4-20) to obtain the composition for enhancing cold resistance based on pyroptosis regulation. The invention further provides a composition obtained based on the preparation method, a preparation and application. The composition provided by the invention can significantly prolong the survival time of acute cold exposure mice; the core body temperature of an acute cold exposure mouse is obviously improved, liver injury is relieved, and liver tissue cell pyroptosis pathway protein expression is regulated.

Application of a high-purity inonotus obliquus polysaccharide component belongs to the technical field of biological medicine, and the high-purity inonotus obliquus polysaccharide component plays a role in preventing and treating by inhibiting autophagy of lung tissue cells and depending on an lncRNA Rmst pathway, and can reduce the number of autophagosomes in lung tissues and the level of conversion from LC3-I to LC3-II; according to the method, the sugar content of IOP can be remarkably increased compared with crude polysaccharide, the protein content is remarkably reduced, four active components with specific molecular weights are obtained through separation, the molecular weight ranges are definite (IOP-g1gt, IOP-g2gt, IOP-g3gt and IOP-g4), and the requirements of medicine research and development for controllable purity and definite components are met.

The invention discloses a culture method of in-vitro uniform monolayer cells of prawns, and belongs to the technical field of biology. The method comprises the following steps: (1) obtaining lymphatic tissues of a last-stage prawn at the early stage of shelling, completely stripping an outer membrane to obtain internal tissues, and cutting the internal tissues into small tissue blocks; (2) cleaning the small tissue blocks, inoculating the small tissue blocks to a culture plate coated with a gelatin solution, adding a complete culture solution into an inoculation system at intervals within 12 hours after inoculation for culture, replacing the complete culture solution 24-48 hours after inoculation, and inducing tissue cells to continuously migrate to form an in-vitro cell layer; (3) blowing and beating the cell culture to obtain a cell suspension, and centrifuging to obtain a cell monolayer; and (4) suspending the cell monolayer by adopting a complete culture solution, then inoculating the cell monolayer into a culture plate coated with a gelatin solution, and culturing to obtain the in-vitro uniform monolayer cells of the prawns. According to the method, the prawn monolayer cells which are long in in-vitro survival time, uniform and stable and are cultured in vitro can be obtained, and an important tool is provided for related virology research.

The application discloses an albumin nano-drug based on HSP90 inhibitor and a preparation method and application thereof, and belongs to the technical field of medicines.The short peptide A6 is modified to human blood albumin through a connecting chain, and the human blood albumin modified by the A6 is self-assembled with geldanamycin derivative G2111 in the presence of an organic solvent, so that the G2111 is combined to the hydrophobic region of the human blood albumin through a non-covalent bond, effective loading of the G2111 is realized, the solubility of the geldanamycin derivative is improved, the drug is targetedly released into cancer cells with high expression of CD44, the targeted accumulation of the drug in tumor tissues is enhanced, damage to other normal tissues and cells caused by off-target effects of the drug is avoided, the albumin nano-drug can be simultaneously used for chemotherapy of leukemia and solid tumors, and has important practical significance.

The application relates to the technical field of biological sample pretreatment, and discloses a high-efficiency separation method of a tissue cell extraction device. The method comprises the following steps: performing parameter calibration and reference loss modeling of a driving motor, and eliminating background interference of an actuator; applying a disturbance signal to a dissociation chamber, locking the characteristic resonance frequency of a tissue sample through frequency domain energy analysis, and performing pulsating crushing to accelerate the dissociation of a matrix structure; extracting the net torque of the motor in real time, deducing the fluid dynamic viscosity, adaptively adjusting the motor speed according to the viscosity drop trend, maintaining the fluid shear force in a preset target interval, and avoiding mechanical damage to cells; calculating the mechanical energy dissipation power in real time, and performing feedforward temperature control compensation to eliminate temperature overshoot caused by thermal conduction lag. The application realizes closed-loop control of the physical phase evolution of a heterogeneous tissue dissociation process, solves the limitation of a fixed parameter in a traditional method, shortens the dissociation time, and significantly improves the single-cell yield and survival rate.

The invention relates to the technical field of biological tissue cell detection, in particular to an in-situ biological tissue cell free radical detection device and method based on EPR. The technical problems that in the prior art, a fluorescent probe is prone to being oxidized, the detection accuracy is affected, a traditional EPR method needs to freeze or fix a sample, a cell sample is prone to being inactivated, and free radicals in the sample are inactivated or attenuated are solved. According to the technical scheme, the in-situ biological tissue cell free radical detection device based on the EPR comprises a culture cabin, a central controller, a culture dish, a cleaning fluid storage rack, a control assembly, a detection assembly and a temperature control assembly; through the design of the movable sterile liquid suction head and the liquid conveying channel, automatic sampling and conveying are achieved, the problem that free radicals are inactivated or attenuated in traditional EPR detection is solved, the authenticity and reliability of a detection result are ensured, and real-time, dynamic and in-situ detection of biological free radicals is achieved through automatic operation of a central control system.

The present disclosure provides compositions and methods for the treatment and prevention of macrophage activation syndrome (MAS) or hemophagic lymphocyte hyperplasia (HLH). The methods involve administering to a patient in need thereof an antibody or fragment thereof that is specific for human GM-CSF protein.

The present invention provides lipid nanoparticles for delivering nucleic acid to spleen tissue that can improve the efficiency of nucleic acid delivery to spleen tissue cells, and a method for delivering nucleic acid to spleen tissue by using same. A lipid nanoparticle for use in delivering a nucleic acid to a spleen tissue, including(A) an ionic lipid represented by the formula (1),(B) an anionic phospholipid or a compound represented by the formula (2),(C) cholesterol, and(D) a dimyristoylglycerol PEG represented by the formula:(definitions of symbols in the formula are as described in the specification), and a method for delivering a nucleic acid to a spleen tissue by using same.

The invention relates to a polypeptide oligonucleotide conjugate with a structure as shown in a formula (I) or (II) or a pharmaceutically acceptable salt or a stereoisomer thereof, and an application of the polypeptide oligonucleotide conjugate or the pharmaceutically acceptable salt or the stereoisomer thereof. The polypeptide oligonucleotide conjugate can be used for inhibiting expression of one or more target genes of central nervous system tissue cells, and has the advantages of good in-vivo effect, long action time and good safety.

The invention provides a preparation method and application of a pilose antler periosteum mesenchymal stem cell exosome.The preparation method comprises the steps that after pilose antler periosteum tissue is obtained, the periosteum tissue is put into a DPBS culture dish containing antibiotics for standby application, then the periosteum tissue is cut up, DPBS is used for cleaning till no blood exists, digestive enzymes are added into the cut periosteum tissue, and the pilose antler periosteum mesenchymal stem cell exosomes are obtained. The preparation method comprises the following steps of: reacting in an incubator containing carbon dioxide to decompose periosteum tissues, adding the periosteum tissues into an alpha-MEM complete culture solution, filtering and centrifuging digested and separated periosteum tissue cells to obtain primary periosteum cells at the bottom layer, and culturing the counted primary periosteum cells by using a culture solution to obtain the mesenchymal stem cells of the pilose antler periosteum, and culturing and filtering the mesenchymal stem cells of the pilose antler periosteum to obtain the exosome of the mesenchymal stem cells of the pilose antler periosteum, which is used for promoting proliferation and repair of human fibroblasts, improving skin quality, promoting hair growth and the like.

The invention relates to the technical field of cell-loaded biological medicine, in particular to a novel efficient nucleic acid delivery method of a carrier siRNA (small interfering Ribonucleic Acid) medicine, which comprises the following steps: constructing a multifunctional nano-carrier taking a biodegradable polymer as a core, the surface of a multifunctional nano-carrier is modified with a targeting ligand, through specific binding of the targeting ligand and a vascular endothelial cell surface receptor and reversible adjustment of penetration promoting molecules on tight connection, the siRNA / carrier compound can efficiently penetrate vascular endothelium including a blood brain barrier, and meanwhile, the siRNA / carrier compound has the advantages that the targeting ligand can be used for preparing a targeted medicine for treating vascular endothelial cells, and the targeted medicine can be used for treating vascular endothelial cells. According to the present invention, the nucleic acid delivery efficiency is significantly improved, the siRNA can be massively delivered into the tumor or the specific tissue cell due to the precise targeting and the efficient penetrating power, and the selected biodegradable polymer and the selected modification molecule have good biocompatibility so as not to cause the obvious immunoreaction and toxicity in the body.

The invention discloses a pig UBC promoter and application thereof, and belongs to the field of gene engineering. The pig endogenous UBC promoter is separated and identified, it is determined through cell tests that the pig endogenous UBC promoter can drive an exogenous gene to be efficiently, stably and widely expressed in pig tissue cells, and the problems that in the transgenic pig product preparation process, the exogenous promoter possibly faces gene silencing, expression inconsistency and low expression efficiency are solved. The porcine endogenous UBC promoter has practical application value in preparation of transgenic pigs.

The application belongs to the technical field of medicine, and relates to a use of minocycline in preparation of an antitumor synergist. The minocycline, as the antitumor synergist, has a synergistic effect with 5-fluorouracil and gemcitabine, significantly enhances the curative effect of an antitumor drug, reduces the dosage of the antitumor drug, and reduces side effects of the antitumor drug on the body or damage of the antitumor drug to normal tissue cells.

The present invention generally relates to the field of recombinant gene expression in host cells. In particular, the invention relates to a recombinant human myeloid-derived growth factor (MYDGF) protein that exhibits a minimal degree of degradation upon expression in a host cell. The recombinant protein is therefore highly suitable for medical use, in particular for treating heart tissue damage and preventing cell death in myocardial tissue. The invention also provides a nucleic acid which encodes the recombinant protein and a host cell that expresses the recombinant protein. The invention also provides a method for producing the recombinant protein in a host cell.

The invention discloses a pelteobagrus fulvidraco brain tissue cell line PYCB sensitive to viruses and application thereof, and belongs to the technical field of aquatic organism cell culture and disease prevention and control. The preservation number of the Pelteobagrus fulvidraco brain tissue cell line PYCB is CCTCC (China Center For Type Culture Collection) NO: C2025148. The Pelteobagrus fulvidraco brain tissue cell line PYCB is successfully constructed by taking the Pelteobagrus fulvidraco brain tissue as an object, and the cell line can be continuously and stably subcultured, is high in proliferation speed and simple in culture method, is sensitive to Pelteobagrus fulvidraco small RNA viruses and Pelteobagrus fulvidraco stem casing viruses, and can be used for isolated culture of viruses. The invention provides an important research platform and an experimental material for separating and identifying fish viruses and virus vaccines and developing medicines.