139 results about "Eukaryotic organism" patented technology

For analysis of the state of a plant (2) or of another eukaryote, an evaluation device or an evaluation system (30) has an evaluation circuit (34) which is designed to carry out an evaluation (36) of spectral data. The spectral data or data generated on the basis thereof by preprocessing are divided into multiple parts and analyzed using an analysis logic. The analysis logic comprises at least one attention mechanism.

The invention belongs to the field of gene engineering, and relates to a DNA binding protein based on TraC effect protein, a fusion protein with a cytosine / adenine base editing function, an editing system and nucleic acid molecules or constructs for encoding the DNA binding protein and the fusion protein. The invention also relates to complexes and kits for gene editing comprising the fusion proteins of the invention or the nucleic acid molecules encoding them. The base editing tool disclosed by the invention can realize efficient, accurate and safe base editing in cells of various eukaryotes such as animals and plants. The unique characteristics of the base editing tool enrich the types of existing base editing tools, and the base editing tool has wide application prospects.

Provided herein are engineered Cas13 proteins with enhanced collateral activity. The engineered proteins comprise RNA binding domains (RBDs) fusions within an active site-proximal loop within a higher eukaryotes and prokaryotes nucleotide-binding (HEPN) domain. Also provided are compositions comprising and methods of using the engineered Cas13 proteins to detect target nucleic acids at attomolar sensitivity.

The invention provides nucleic acids, polypeptides, transgenic plants, compositions and methods for conferring pesticidal activity (e.g., insecticidal activity) to bacteria, plants, plant cells, tissues and seeds. Nucleic acids encoding the insecticidal proteins can be used to transform prokaryotic and eukaryotic organisms to express the insecticidal proteins. The recombinant organisms or compositions containing the recombinant organisms or insecticidal proteins or in combination with an appropriate agricultural carrier can be used to control an insect pest in various environments.

The invention relates to the field of genetic engineering in biotechnology, in particular to a high-precision base editor capable of expanding the editing range and improving the editing efficiency. According to the editor, the Cas9 variant SpRY which is not limited by PAM and cytosine deaminase CDA1 with different truncation lengths are fused, the CDA1 delta-SpRY-BE3 high-precision editor which is wider in editing range and higher in editing efficiency is constructed, and the editor can effectively edit any cytosine on a genome while keeping high editing precision. Tests in yeast and rice cells show that compared with an existing standard editor CDA1-SpRY-BE3, the editor has the advantages that the editing efficiency is remarkably improved on multiple target spots, the application value of the editor in higher eukaryotes is proved, and a more efficient and accurate tool is provided for genetic disease treatment and crop breeding.

The invention belongs to the technical field of gene engineering, and particularly relates to a biotin ligase mutant as well as a coding gene, an expression vector, a recombinant cell and application thereof. The amino acid sequence of the biotin ligase mutant is as shown in SEQ ID NO. 2. The biotin ligase (Bar A) mutant provided by the invention can be efficiently expressed in eukaryotes, the expression level of the biotin ligase (Bar A) mutant is obviously improved in mammalian cells compared with a wild type, and the biotin ligase (Bar A) mutant has good catalytic activity after expression, can effectively catalyze biotinylation of protein containing a biotin receptor sequence, and has good application prospects. The method has a wide application prospect in the fields of biotinylated protein preparation, and protein labeling, positioning, immunodetection, affinity purification, function research and the like based on a biotin-avidin system (BAS).

The present invention relates to a modified stem cell and an ex vivo method for preparing defined mature cells from genetic programming of said modified stem cell which can be carried out in absence of certain growth factors, as well as a kit allowing to transform stem cells, and the different uses of the mature cells, for example for in vitro drug screening and toxicology or as cultivated meat. The genetic programming involves a molecular switch between two mutually exclusive programs of stem cell expansion and differentiation. The invention may be used for stem cells or other progenitors cells of any type, from any eukaryotic organism, but finds particular application in pluripotent stem cells from humans and livestock animals.

The present invention generally relates to systems (100) and methods for generating, managing, and displaying non-fungible digital representations (102) of living, biological, and / or eukaryotic organisms (104). More specifically, the systems (100) and methods for generating, managing, and displaying non-fungible digital representations (102) include genetic material (106), such as single nucleotide polymorphisms. The systems (100) and methods can record genetic data from the genetic material (106) on a blockchain (114) and can generate and display the non-fungible digital representation (102) of the living, biological, and / or eukaryotic organisms (104) that are based on the genetic data on the blockchain (114).

The invention relates to the field of nucleic acid editing, discloses a Cas protein, a combined protein, a nucleic acid molecule, a ribonucleoprotein compound, a recombinant vector, a transgenic cell and application thereof, and particularly discloses the Cas protein, the combined protein, a gene for coding the Cas protein or the combined protein, the nucleic acid molecule, the ribonucleoprotein compound and the recombinant vector. The invention discloses a transgenic cell, application thereof and a gene editing method. The Cas protein and the crRNA component thereof provided by the invention can perform specific targeted editing on genome DNA in eukaryotes and have wider target recognition, and the off-target potential of the Cas protein and the crRNA component thereof can be reduced by virtue of the PAM characteristics of four bases of the Cas protein and the crRNA component thereof.

To provide a composition used for targeted genome editing in a eucaryotic cell.SOLUTION: There is provided a composition for introducing a Cas9 / RNA composite into an eucaryotic cell for modifying a targetted endogenous nucleic acid sequence, the composition including: a recombined Cas9 protein; a single strand guide RNA; and the Cas9 / RNA composite formed by at least parts of the recombinant Cas9 protein and the single strand guide RNA, where the single strand guide RNA is included by at least a two-fold molar excessive amount with respect to the recombinant Cas9 protein. The Cas9 / RNA composite is formed by combining the recombinant Cas9 protein and the single strand guide RNA, the Cas9 / RNA composite forms a composite before being introduced into the eucaryotic cell, the single strand guide RNA is an RNA or synthesized RNA transferred in vitro, and the combination of the recombinant Cas9 protein and the single strand guide RNA may cause modification of the target endogenous nucleic acid sequence in a nucleus of the eucaryotic cell.SELECTED DRAWING: Figure 1a

The invention relates to the technical field of bioinformatics and molecular cytogenetics, in particular to a method for counting centromeres of chromosomes based on a Nuf2 antibody and application. The method comprises the following steps: performing chromosome centromere counting on a Nuf2 antibody and a centromere specific protein Nuf2 through an immunofluorescence reaction, so that the number of chromosome centromeres can be directly observed, and the number of chromosomes can be efficiently and quickly determined. Besides, due to the fact that the specific protein Nuf2 of the centromere is highly conservative in eukaryotes, the invention further provides application of the method to identification of the plant ploidy, the method is easy and convenient to operate and low in cost, the ploidy identification efficiency is remarkably improved, and a universal technical platform is provided for genetic breeding.

The present invention provides a versatile platform system for regulating gene expression in cells of eukaryotic organisms, including plants, algae and fungi, and mammals. A platform system of the invention includes an expression control element and expression system comprising the same. Expression control elements according to the invention can be inserted into a variety of cell types having the capability of alternative splicing and can be regulated by a polyamine or polyamine analogues. Host cells comprising the expression control elements of the invention are typically grown in a cell or tissue culture, or form part of a living plant, algae, or fungi.

The present invention relates to Split-Intron Final Self-Inactivating (SIN) retroviral vectors which comprises a viral major splice donor (mSD) comprising mutations, a mouse mammary tumor vector long terminal repeat (MMTV-LTR) or a Type B leukemogenic virus long terminal repeat (TBLV-LTR), a eukaryotic splice acceptor (eSA), and a eukaryotic splice donor (eSD). Furthermore, the invention relation to the use and methods of uses of such vectors in gene therapy.

The invention provides a SpuFz1 variant derived from eukaryotes and application of the SpuFz1 variant in gene editing, particularly provides the SpuFz1 variant and modified omega RNA, and finds that the modified omega RNA and SpuFz1 mutant combination can remarkably improve the editing efficiency and greatly reduce the off-target rate for the first time.

The invention discloses base-modified mRNA (messenger Ribonucleic Acid) and application thereof in a cell-free protein synthesis system, and belongs to the technical field of nucleic acid modification. Natural nucleoside triphosphate is completely replaced by different types of basic group modified nucleoside triphosphate, mRNA with different chemical modifications is obtained through an in-vitro transcription method and purification, and the mRNA is put into an in-vitro cell-free protein synthesis system for reaction. A reaction element recombination system-PURE system based on prokaryote Escherichia coli purified protein and an eukaryote CFPS system taking rabbit reticulated red lysate as a material are mainly selected to detect the corresponding translation efficiency of mRNA modified by different bases in two cell-free protein synthesis systems respectively. Different regulatory activities may be shown in different cell-free protein synthesis systems when different modified nucleotides are doped into mRNA, base modified triphosphoric acid capable of improving the translation efficiency of an in-vitro protein synthesis system is screened out, and the stability of mRNA in the cell-free protein synthesis system and the corresponding protein expression level are improved.

The Quadruplet Expanded DNA (QED) eukaryote genetic code comprising twenty nondegenerate QED codons encode proteins (the protein-encoding codons), and thirty-five nondegenerate QED codons (the noncoding codons) being highly correlated with cis-regulatory elements control and regulate transcription, alternate splicing, and polymerization in eukaryotic protein synthesis using canonical amino acids. The QED eukaryote genetic code is an advancement to gene therapeutics that allows for the correction of dysfunctional proteins. Additionally, the QED eukaryote genetic code is further applicable for changing paradigms relating to identifying cures for monogenic rare, multigene cancer, and neurodegenerative diseases.

An engineered tyrosyl-tRNA synthetase / tRNA pair that co-translationally incorporates O-sulfotyrosine in response to UAG codons in E. coli and mammalian cells is described herein. This platform enables recombinant expression of eukaryotic proteins homogeneously sulfated at chosen sites.

Novel combinations of cysteine-rich insecticidal proteins (CRIPs) and insecticides (IAs), such as chemicals, molecules, nucleotides, polynucleotides, peptides, polypeptides, proteins, toxins, toxic substances, poisons, insecticides, pesticides, organic compounds, inorganic compounds, prokaryotes or eukaryotes (and substances produced from such prokaryotes or eukaryotes), are described and claimed for the control and / or eradication of pests. [Solution] Novel insecticide combinations, compositions, and methods for using them are provided. The present invention relates to a combination of a cysteine-rich insecticide peptide (CRIP) and an insecticide (IA). The present invention also describes the use of the combination and composition for controlling insects. This specification describes the gene encoding CRIP, compositions and combinations containing CRIP and IA, and methods useful for controlling pests using them.

A method for improving the replication of covalent closed circular plasmids is provided. [Solution] The method comprises the step of preparing a covalent closed circular plasmid having a Pol I-dependent origin of replication and an insert containing a structured DNA sequence selected from the group consisting of a reverse repeat sequence, a directional repeat sequence, a homopolymer repeat sequence, a eukaryotic origin of replication, or a eukaryotic promoter enhancer sequence, wherein the structured DNA sequence is located at a distance of less than 1000 bp from the Pol I-dependent origin of replication in the direction of replication. The method also comprises the step of modifying the covalent closed circular recombinant molecule so that the Pol I-dependent origin of replication is replaced with a Pol III-dependent origin of replication, thereby improving the replication of the covalent closed circular plasmid with the resulting Pol III-dependent origin of replication. A covalent closed circular recombinant DNA molecule without antibiotic markers is also provided.

Disclosed are split complementary base editing systems based on bimolecular deaminases and uses thereof. A split complementary base editing system mainly includes base editing fusion proteins A and B that are from splitting at a deaminase domain embedded inside a Cas9 nickase (nCas9), and a guide RNA (gRNA). The present disclosures of split complementary cytosine base editing systems can greatly reduce Cas9-dependent and Cas9-independent off-target effects in the genome while maintaining robust on-target cytosine base editing. The present disclosures also provide the split complementary adenine base editing systems. The split complementary cytosine (adenine) base editing systems are widely applicable to various eukaryotic organisms, and can be used in crop genetic breeding, animal breed improvement, and even clinical treatment of human genetic diseases.

The invention belongs to the field of gene engineering. Specifically, the invention relates to a modular gene editing tool and application thereof. Specifically, the invention provides a modularized gene editing tool protein capable of accurately editing a genome nucleotide sequence of an organism, a gene editing system comprising the protein and a method for performing gene editing by using the system. The modular gene editing tool disclosed by the invention can realize efficient, accurate and universal gene editing in cells of various eukaryotes such as animals and plants. The types of existing editing tools are enriched, and the method has a wide application prospect.

The present invention. relates to a disinfectant composition against parasitic organisms. The invention also relates to a method for disinfecting at least one area to prevent parasitosis. The invention also relates to the use of at least one compound selected from fatty acids and fatty alcohols and which have a chain length of to carbon atoms in a disinfectant composition for improving penetration of active compounds contained in said composition into the durable forms, in particular eggs or oocysts, of parasitic organisms.

The present invention relates to an oligonucleotide comprising: a) a nucleotide sequence of a broom-kojin-ricin loop (SRL) of a eukaryotic / mammalian 60S ribosomal subunit wherein the SRL nucleotide sequence comprises at least one adenine; and b) at least one cut-dependent marker; wherein the oligonucleotide is a single strand. Preferably, the oligonucleotides form at least one loop structure or stem-loop structure. In another aspect, the invention relates to a method of detecting active shiga toxin in a sample, the method comprising the steps of: a) providing at least one oligonucleotide according to the invention; b) providing a sample of shiga toxin to be detected; c) incubating the sample with at least one single-stranded oligonucleotide; and d) detecting a labeled signal, wherein the signal indicates the presence of Shiga toxin in the sample. The invention additionally comprises a kit comprising at least one oligonucleotide according to the invention.

Provided are: a nucleic acid cleavage enzyme which comprises a nuclease domain having a specific amino acid sequence and a nucleic acid binding domain; a use of the nucleic acid cleavage enzyme; a method for producing a variant of a eukaryotic cell, the method comprising deleting a centromere region of a genome on a chromosome in the eukaryotic cell; a use of the method; a vector or a DNA fragment for use in engineering of a gene of a eukaryotic organism, the vector or the DNA fragment including a telomeric repeat sequence; and a use of the vector or the DNA fragment.

The present invention relates to a disinfectant composition against parasitic organisms. The invention also relates to a method for disinfecting at least one area to prevent parasitosis. The invention also relates to the use of at least one compound selected from fatty acids and fatty alcohols and which have a chain length of 10 to 26 carbon atoms in a disinfectant composition for improving penetration of active compounds contained in said composition into the durable forms, in particular eggs or oocysts, of parasitic organisms.

The present application provides a kind of eukaryotic pan-transcriptome annotation method, comprising the following steps: the transcriptome data of more than 3 different sources of eukaryotic species sample is compared to reference genome, obtains corresponding gene structure annotation information, integrates, obtains the transcriptome annotation result of reference genome dependence;Sample transcriptome data is spliced and assembled, and corresponding mature transcript sequence information is obtained, and the mature transcript sequence information is compared with reference genome, and corresponding gene structure information is obtained, to obtain the transcriptome annotation result of reference genome independence;Integrate structural transcript annotation information, filter transcript that cannot be compared with reference genome, obtain new transcript annotation information;Integrate sample high-throughput transcriptome data, and the pan-transcriptome annotation information of target eukaryotic species can be obtained.The present application can obtain more complete transcriptome annotation structure of eukaryote, and different strategies are mutually verified, which greatly improves the annotation accuracy.

An engineered tyrosyl-tRNA synthetase / tRNA pair that co-translationally incorporates O-sulfotyrosine in response to UAG codons in E. coli and mammalian cells is described herein. This platform enables recombinant expression of eukaryotic proteins homogeneously sulfated at chosen sites.

To perform a status analysis of a plant or other eukaryote, an evaluation device or system (30) includes an evaluation circuit (34) configured to perform an evaluation (36) of spectral data. The evaluation includes at least one attention mechanism.