106 results about "Targeted Modification" patented technology

The present disclosure provides compositions and methods for the targeted modification of RNA molecules by RNA prime editing. The compositions and methods may be conducted invitro or in vivo within cells (e.g., human cells) for the therapeutic correction of disease-causing mutations and / or installation of motifs or mutations in RNA molecules of interest as a tool for scientific research. The disclosure provides compositions and methods for conducting RNA prime editing of a target RNA molecule (e.g., an RNA transcript) that enables the incorporation of one or more nucleotide changes and / or targeted mutagenesis of a target RNA molecule. The nucleotide change can include a single-nucleotide change, an insertion of one or more nucleotides, or a deletion of one or more nucleotides. More in particular, the disclosure provides a variety of configurations of the RNA prime editors each comprising a nucleic acid programmable RNA binding proteins (napRNAbp), such as Cas13, and an RNA-dependent RNA polymerase (RDRP), which are provided as fusion proteins or which can be separately provided in trans. The RNA prime editors are guided to a target RNA site by a guide RNA, which can be a rpegRNA that includes a template region for the synthesis of an RNA sequence to be installed on the RNA molecule attached to an available 3′ terminus. In others embodiments, the RNA template can be provided in trans.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat transcription factor TaWRKY22 and a coding gene thereof in regulation and control of plant stripe rust resistance. The function of the wheat transcription factor TaWRKY22 is researched through molecular biology and genetics methods, it is found that knockout of the TaWRKY22 gene significantly improves the resistance of wheat to stripe rust, overexpression of the TaWRKY22 gene reduces the resistance of wheat to stripe rust, and then it is indicated that the wheat transcription factor TaWRKY22 plays a negative regulation role in wheat stripe rust resistance. Therefore, the TaWRKY22 gene is subjected to targeted modification through a gene editing technology, a new genetic material and a molecular breeding strategy can be provided for wheat stripe rust resistance breeding, and an important material and technical support are provided for breeding of wheat disease-resistant varieties.

The invention provides a modification processing method and processing system based on beam shaping, and the method comprises the following steps: obtaining three-dimensional light field distribution corresponding to a three-dimensional model through a holographic algorithm according to the three-dimensional model of a target modification structure, and generating a phase modulation diagram; loading the phase modulation diagram through a spatial light modulator, and modulating the wavefront phase of the incident laser, so that a light beam is focused on the surface of the material or in the material to form a preset spatial curved surface light field; and the spatial curved surface light field is focused on the material, so that the material in the light field region is subjected to nonlinear absorption or phase change, and a modified structure of a three-dimensional model is directly formed. According to the method, the limitation of traditional point-by-point scanning type machining is broken through, the machining efficiency and flexibility of a complex three-dimensional structure are remarkably improved, the method is suitable for scenes such as transparent material internal waveguide inscribing, curved surface splinter separation, large-area stripping, functionalized surface preparation and opaque material huge removal machining, and the machining efficiency and quality are remarkably improved.

The invention relates to the technical field of biomedical engineering and drug delivery, and particularly discloses a novel targeted drug delivery system based on rose fruit extracellular vesicles, which comprises the following steps: preparing rose fruit source extracellular vesicles; preparing a medicine carrying vesicle; preparing targeted modified drug-loaded vesicles; according to the application disclosed by the invention, a stem related pathway is specifically down-regulated through the components of the rose fruits, and meanwhile, conventional tumor cells are killed by using adriamycin, so that a complementary treatment mechanism is formed, and the tumor recurrence risk is fundamentally reduced. The phenylboronic acid group of DSPE-PEG-PBA is used for specifically recognizing sialic acid over-expressed on the surface of a tumor cell, so that the enrichment of the medicine in tumor tissues is remarkably improved, and meanwhile, the distribution of normal tissues is reduced. By means of the natural biocompatibility and low immunogenicity of the RHNVs and in combination with the long circulation characteristic of a PEG chain, the delivery efficiency is guaranteed, the systemic toxicity is reduced to the maximum extent, and particularly the cardiotoxicity and myelosuppression of adriamycin are relieved.

The invention discloses a copywriting modification method and device, a terminal and a storage medium, and relates to the technical field of artificial intelligence. The method comprises the steps of generating a second evaluation standard for an initial copywriting and a first evaluation standard through a copywriting evaluation model; evaluating the initial copywriting according to the second evaluation standard to obtain a target evaluation opinion; and generating a target modification opinion based on the target evaluation opinion. According to the document evaluation model, the initial first evaluation standard is supplemented and perfected, and a more comprehensive second evaluation standard is generated. The initial copywriting is evaluated based on the second evaluation standard, accurate target evaluation opinions can be output to clarify specific problems existing in the copywriting, targeted target modification opinions are generated according to the target evaluation opinions, and automatic modification of the copywriting can be achieved. The problems that the manual modification level is difficult to keep consistent and the cost is high are solved.

The application discloses a method for producing deoxythymidine triphosphate by using a recombinant strain, and comprises the following steps: step 1: using a CRISPR / Cas9-mediated gene editing method to perform targeted modification on an E. coli genome, and the targeted modification comprises the following contents: a. knocking out a nucleoside triphosphate pyrophosphatase gene (mazG / yhdE) to block a path of degrading dTTP into dTMP; b. knocking out a nucleotide nucleosidase gene (ppnN) to block a path of degrading dTMP into thymine; and c. knocking out a nucleotidase gene (yfbR / yfdR / ushA / umpH / umpG / yjjG) to block a path of degrading dTMP into dT. The application provides a method for producing deoxythymidine triphosphate by using a recombinant strain, and the conversion rate of the method reaches 80% to 90%, and after ion exchange chromatography purification, the purity can reach more than 99%.

This invention relates to the field of metal material strengthening technology, specifically a method and system for enhancing the wear resistance of gate valves based on surface modification. The method includes: receiving a gate valve strengthening command; determining a target gate valve based on the command; identifying a set of target modification regions based on the target gate valve; performing surface pretreatment on multiple target modification regions within the target modification region set to obtain multiple pretreated modification regions; setting multiple powder mass ratios; constructing multiple laser cladding parameters; obtaining multiple cladding samples based on the laser cladding parameters and powder mass ratios; performing cladding performance testing on the multiple cladding samples to obtain multiple performance scores; determining a target cladding parameter combination based on the performance scores; and performing laser cladding on the multiple pretreated modification regions using the target cladding parameter combination to obtain a wear-resistant strengthened gate valve. This invention can improve the wear resistance of gate valves and extend their service life.

A duchenne muscular dystrophy-related exonic splicing enhancer, sgRNA and gene editing tool can be applied in the preparation of drugs for treating duchenne muscular dystrophy. The gene editing tool designed on the basis of cytosine deaminase AID mutants and Cas9 mutants can perform site-specific modification on a mammalian genome by using an adeno-associated virus (AAV) as a vector. By optimizing an encoding nucleic acid sequence and an element composition structure of the editing tool, site-specific targeted modification of mammalian genetic material DNA can be efficiently achieved; and by performing targeted genetic manipulation on the nucleic acid sequence carrying disease mutations, a pathogenic mutation cannot be retained in a mature protein amino acid sequence or the pathogenic mutation cannot perform its function, so that the purpose of treating various gene mutation type genetic rare diseases is achieved, and the advantages of high efficiency, safety and stability are achieved.

The invention relates to the field of computer data processing, and discloses a data integration method, device and equipment, and the method comprises the steps: generating equipment identification information corresponding to a plurality of pieces of computer equipment associated with a target user, and building an equipment information list; and if the equipment information of the current computer equipment is matched with the equipment information list, calling real-time modification data of the current computer equipment, carrying out modification action identification on the real-time modification data, obtaining target modification data corresponding to the target user, and carrying out data integration and updating on cloud storage data of the target user. According to the data integration method, device and equipment of the computer equipment, the computer equipment and the operation user generating modified data are synchronously recognized in the data integration process, the pertinence and integration efficiency of the data are effectively improved, and meanwhile, the user can conveniently, rapidly and accurately inquire the needed data.

The invention belongs to the technical field of biological materials, and particularly relates to a pathogen-targeted engineered exosome as well as a preparation method and application thereof. The engineered exosome provided by the invention comprises an adipose-derived stromal cell exosome, antibacterial peptide M27-39 and 6 '-sialic acid lactose, and integrates two functions of resisting influenza virus and relieving viral pneumonia, targeted modification of 6'-SL can simulate a host receptor structure, and binding of HA and sialic acid is competitively blocked. After the antibacterial peptide M27-39 carried by the exosome is released in the lung, the direct antiviral effect can be achieved, excessive inflammatory response induced by viruses is inhibited, and meanwhile lung injury caused by influenza virus infection is relieved by promoting alveolar epithelial cell repair and relieving pathological injury of viral pneumonia. Therefore, the engineered exosome provided by the invention can be combined with pathogenic microorganisms in a targeted manner, and has obvious functions of resisting pathogenic microorganisms, resisting inflammation, repairing barriers and intervening the pathological process of infectious lung injury.

The invention discloses a carbonyl reductase directional modification method for synthesis of atorvastatin intermediates, which belongs to the technical field of enzyme gene engineering, and is characterized in that carbonyl reductase genes are used as templates, mutation primer pairs are designed, double-site mutation is carried out by adopting an overlapping extension PCR (polymerase chain reaction) method, escherichia coli is transformed, IPTG (isopropyl-beta-d-thiogalactoside) is used for inducing expression, and mutant carbonyl reductase is prepared. The K205D / R237W double mutation modified carbonyl reductase improves the enzyme activity, combines the synergistic effect of the thiophene pyridine coenzyme analogue, improves the conversion rate of the atorvastatin intermediate and the product purity, is significantly superior to the traditional chemical reduction method, avoids the low-temperature and high-pressure conditions of the traditional chemical method, reduces the energy consumption, and is suitable for industrial production. The use amount of an organic solvent and the generation amount of byproducts are reduced, and the generation amount of wastewater is reduced.

The invention discloses application of a thermal induction type RCA1 gene in improving heat resistance of plants. The nucleotide sequence of the thermal induction type RCA1 gene is shown as SEQ ID NO. 1. The RCA1 gene disclosed by the invention has the function of maintaining the photosynthesis efficiency of plants under high-temperature stress, enriches the heat-resistant molecular theory of the azalea, and has important practical significance in improving the heat-sensitive defect of the azalea, improving the cultivation efficiency and improving the adaptability and attractiveness of the azalea in landscape architecture. In addition, the application of the RCA1 gene is not limited to rhododendron, can also be used for heat resistance improvement of other plants, and is of great significance to plant heat resistance oriented transformation.

The invention relates to the technical field of fusion membrane nano preparations, and provides a nano preparation capable of being used for cerebral arterial thrombosis gene drug targeted delivery, which comprises lipid-calcium phosphate nanoparticles, which are composed of DOPA modified calcium phosphate cores and lipidosome and are used for providing positive charges to obtain efficient gene loading efficiency and proton sponge effect, the intracellular efficient delivery and release of the gene are realized; and the exosome membrane is prepared from a cell culture solution extracted from bone marrow-derived mesenchymal stem cells and is used for improving the transfection efficiency of the carried medicine. According to the present invention, by using the process characteristic that the inner layer phospholipid and the outer layer phospholipid are not synchronously prepared during the LCP preparation process, the biological membrane of the exosome and the LCP outer layer phospholipid are fused during the LCP preparation of the outer layer phospholipid so as to construct the fusion membrane preparation having the characteristics of the biological membrane and capable of carrying out AMD3100 targeting modification, such that the advantages of the biological membrane and the AMD3100 in the targeting delivery system are combined so as to achieve the target delivery effect; and the bottleneck of drug delivery for brain diseases is solved.

The invention discloses a modified exosome as well as a preparation method and application thereof, and relates to the technical field of biological medicines, CD44 targeted modification and composite active ingredients are synergistic to realize efficient repair of burn wounds and sensitive skin, and the modified exosome can be specifically enriched on the burn wounds or damaged sensitive skin parts through CD44 targeted peptide fragment coupling, so that the effect of repairing the burn wounds and the sensitive skin is achieved. The defects that a traditional repairing product is poor in targeting property and low in active ingredient utilization rate are overcome, the loaded dipotassium glycyrrhizinate, glutathione and FGF form a synergistic effect, wound healing and skin barrier repairing can be promoted through the FGF, the dipotassium glycyrrhizinate and the glutathione can be used for strongly resisting inflammation and oxidation, release of sensitive skin inflammatory factors is reduced, and the repairing effect is achieved. By matching with nitrogen protection incubation and a pulse ultrasonic loading process, the stability of active ingredients is greatly improved, finally, the repair effects that the healing process of burn wounds is remarkably accelerated, and sensitive skin percutaneous moisture loss is obviously reduced are achieved, and safety and effectiveness are both considered.

The application discloses a kind of targeted drug-loaded nanoparticles response release performance detection method, specifically related to targeted nano-drug detection field, for solving the problem that only according to single release curve in the release performance detection of existing targeted drug-loaded nanoparticles, it is difficult to distinguish ordinary environment early leakage, insufficient release of channel plugging and stimulation response release. By generating a structure reference record containing initial drug loading, targeting modification amount and channel plugging state, and dividing the homologous sample into unmodified drug-loaded control group, targeted drug-loaded detection group and targeted modification blank correction group, and then combining the non-stimulation leakage curve, the stimulation switching release curve and the corrected release curve, the effect of distinguishing early leakage, stimulation response release and channel plugging effect is achieved, so that the detection result more accurately reflects the plugging leakage reduction ability and stimulation release ability of targeted drug-loaded nanoparticles.

The invention belongs to the technical field of biology and medicine, and particularly relates to a tumor targeted photo-thermal chemotherapy drug delivery system and a preparation method and application thereof. According to the tumor targeted photo-thermal chemotherapy drug delivery system provided by the invention, a core carrier adopts a black phosphorus nanosheet (BPNS) and ferroferric oxide (Fe3O4) composite structure, BPNS generates heat under irradiation of near-infrared light to realize photo-thermal therapy, and Fe3O4 endows a magnetic targeting function; the medicine part is a ferroptosis inducer RSL3, glutathione (GSH) is reduced and reactive oxygen species (ROS) is increased through an inhibition system Xc-, and tumor cell ferroptosis is induced; the targeting modification layer is liver cancer cell membrane protein (Pro), and liver cancer cell surface receptors are specifically recognized through a homologous targeting mechanism, so that the enrichment efficiency of drugs in tumor tissues is improved. The system can significantly improve the treatment effect and reduce the toxicity to normal tissues, and provides a new solution for liver cancer treatment.

The invention discloses a nano-drug for promoting cupric death of tumor cells and a preparation method of the nano-drug. The nano-drug comprises hyaluronic acid; a dopamine; copper ions; a diethyldithiocarbamic acid-copper conjugate; and a method for preparing the same. A peptide fragment with a targeting function; modifying a thiol ligand; the preparation method comprises the following steps: preparing a diethyl dithiocarbamic acid-copper conjugate, forming a nano-structure inner core, wrapping the conjugate, forming a copper ion protection layer, and purifying and carrying out targeted modification on a nano-drug. The preparation method comprises the following steps of: preparing the diethyl dithiocarbamic acid-copper conjugate, forming a nano-structure inner core, wrapping the conjugate, forming a copper ion protection layer, and carrying out targeted modification on the nano-drug. The copper ions are further fixed through the coordination effect of dopamine and the copper ions, wrapping of the diethyl dithiocarbamic acid-copper conjugate and the thiol-copper coordination effect of the modified thiol ligands, so that a double-coordination mechanism is formed, the loading capacity of the copper ions in the nano-drug is remarkably improved, and the stability of the nano-drug is improved. The copper death of tumor cells can be more effectively triggered, the copper ion loading capacity can be improved, and a material basis is provided for the efficient copper death effect.

The invention provides a retina photosensitive neuronal lesion zebrafish model and a construction method thereof, and relates to the technical field of gene engineering. Wherein the sgRNA comprises a guide sequence which can specifically recognize and be combined with a nucleotide sequence shown as SEQ ID NO.4 in an elovl2 gene. The high specificity of the sgRNA ensures the precise targeted modification of the elovl2 gene, and obviously reduces the off-target risk. The genetic background of the constructed zebrafish model is clear, and the retinopathy phenotype can be reliably attributed to the lovl2 gene mutation, so that a stable biological tool which is highly similar to human diseases is provided for related disease research and drug screening.

The invention discloses a precise editing method of a gamma-PGA high-yield strain based on CRISPR-Cas9n, and relates to the technical field of high-yield strains. According to the invention, a CRISPR-Cas9n double-nickase system is adopted, and adjacent DNA nicks are generated through the synergistic effect of double sgRNA, so that the non-specific cutting risk is greatly reduced; the double sgRNA targeting design is combined with a homologous recombination template, so that directional editing of key genes is realized, the homologous recombination efficiency reaches 75 + / -5%, and target modification is ensured to be stably integrated into a genome; by virtue of a PEG-mediated protoplast conversion method, the limitation of low efficiency of traditional electrotransfection is overcome, and the CRISPR carrier is rapidly removed in combination with a temperature-sensitive plasmid elimination system, so that cytotoxicity caused by continuous expression is avoided; under optimized fermentation conditions, the gamma-PGA yield of the edited strain reaches 15 + / -2g / L and is increased by 200% compared with that of the original strain.

The disclosure describes novel systems, methods, and compositions for the manipulation of nucleic acids in a targeted fashion. The disclosure describes non-naturally occurring, engineered CRISPR-Cas systems, components, and methods for targeted modification of nucleic acids such as DNA. Each system includes one or more protein components and one or more nucleic acid components that together target nucleic acids.

The invention relates to the technical field of biological medicine, and particularly discloses a lipidosome targeting SELENOP + macrophages, a preparation method of the lipidosome and application of the lipidosome in lung squamous cell carcinoma treatment. According to the lipidosome, an SELENOP antibody is used as a targeted modification molecule, si-SELENOP and s-ABCA1 / G1 are loaded, and accurate delivery of siRNA in target cells is achieved through specific binding of the antibody and a SELENOP receptor on the surface of a SELENOP + macrophage. The targeting liposome prepared by the invention can specifically inhibit cholesterol efflux pathways of SELENOP + macrophages, reverse tumor phenotypic polarization, remodel a tumor immune microenvironment and recover anti-tumor activity of immune cells such as CD8 + T cells, NK cells and the like. When the liposome is independently used or combined with a PD-1 antibody, the growth and metastasis of the lung squamous cell carcinoma can be remarkably inhibited, the treatment effect is improved, a brand new metabolism-immunity synergistic targeted treatment strategy is provided for the lung squamous cell carcinoma, and the liposome has a good clinical application prospect.

The invention belongs to the technical field of genetic engineering and plant genetic breeding, and particularly relates to a gene for regulating and controlling the cold tolerance of rice in the seedling stage and application of the gene. In order to excavate the gene for regulating and controlling the cold resistance of the rice, the nucleotide sequence of the rice gene OsCYP75B3 is mutated by virtue of a CRISPR / Cas9 genome targeted modification technology, and an experimental result shows that the cold resistance of the mutated rice in the seedling stage is remarkably reduced, so that the gene OsCYP75B3 is proved to negatively regulate and control the cold resistance of the rice in the seedling stage; the functional gene for regulating and controlling the cold resistance of the rice in the seedling stage is determined for the first time, and an important reference is provided for analyzing a molecular mechanism of the cold resistance of the rice in the seedling stage; based on the discovery, it is expected that the expression level of the gene OsCYP75B3 is improved through an overexpression technology, the gene OsCYP75B3 is applied to rice genetic engineering breeding, the cold resistance of rice is finally enhanced, and a key technical support is provided for guaranteeing grain safety.

The invention relates to the technical field of biological detection, and particularly discloses a detection method of a bionic nano-carrier system based on a GPC3 engineering macrophage membrane in liver cancer treatment, and the detection method comprises the steps of S1, nanoparticle preparation, S2, drug encapsulation, S3, macrophage membrane MM preparation, S4, nanoparticle and macrophage membrane combination, S5, GPC3 targeted modification, and S6, in-vitro cell experimental verification. Through modification of the GPC3 targeting peptide, the nanoparticles can specifically target liver cancer cells, so that the targeting property of liver cancer treatment is greatly improved, and damage to normal tissues is reduced; the use of the macrophage membrane effectively avoids the phagocytosis of an immune system, enhances the circulation time and stability of the drug in vivo, improves the immunotherapy effect, and can significantly induce ferroptosis of liver cancer cells in combination with sorafenib (Sor) and astragaloside IV (As), thereby providing a novel treatment strategy and improving the treatment effect of liver cancer.

This application relates to the field of game and animation production technology, and in particular to a method for modifying the appearance of animated characters, an electronic device, and a storage medium. This application first identifies the target driving bone from the skeletons of the target character model and adds a modification control component to the target driving bone; it then identifies the target modification controlled object associated with the target driving bone from a pre-set candidate control list; it captures bone pose transformation commands through the modification control component and determines the initial pose data, target pose data, and process pose data of the target character model based on the bone pose transformation commands, and then performs pose distance analysis to obtain a pose distance matrix; it performs radial basis function interpolation calculation based on the pose distance matrix to determine the corresponding modification weights for the process pose data; and finally, it performs character modification on the target modification controlled object to obtain the target animation of the modified character model. This method improves visual expressiveness while ensuring animation production efficiency.

The invention discloses a method for overcoming tumor immune desert-type drug resistance by using a nano-carrier delivery vaccine. The method comprises the following steps: realizing targeted immune activation and microenvironment remodeling by constructing a three-stage structure collaborative delivery platform; the system comprises core-shell lipid nanoparticles (LNP) constructed based on a liposome self-assembly technology, mesoporous silica nanoparticles (MSNs) are adopted as a slow release module, and a manganese dioxide (MnO2) nano-cluster is anchored by a surface modified amino column [6] arene-lactose pyridine host-guest complex through a pi-pi accumulation effect; according to the present invention, through the dual targeting modification, the tumor cell membrane camouflage (retaining EGFR, CD44 and other membrane antigens) and the ApoA1 mimic peptide (22A peptide) synergistically act, the enrichment amount of the nanometer vaccine in the tumor tissue is 5.2 times of the normal liver tissue, and the problems that the traditional vaccine difficultly penetrates through the compact matrix of the immune desert type tumor and the lymph node activation efficiency is low are solved.

The invention discloses a berberine nano material as well as a preparation method and application thereof, and belongs to the technical field of nano materials. According to the preparation method provided by the invention, EGCG and BBR are combined with a specific nano-carrier to obtain the berberine nano-material capable of remarkably improving the water solubility of berberine and greatly improving the bioavailability of the berberine, and then the berberine nano-material can be accurately enriched at a renal lesion part through a targeted modification technology, so that the bioavailability of the berberine is greatly improved. The treatment effect on kidney diseases is enhanced, and meanwhile, the distribution of medicines in other organs is reduced, so that the side effects are reduced. The preparation method disclosed by the invention is simple and convenient, industrial production is convenient to realize, and the prepared product provides an efficient, safe and economical novel drug delivery scheme for clinical treatment of kidney diseases.

A neural network-guided method for precise control of material modification, relating to the field of material modification technology, addresses the problem of uneven surface modification caused by the inability of existing plasma modification techniques to precisely control plasma parameters for nonlinear phase transitions in microstructures. This invention first obtains the initial state parameters and target modification parameters of the material to be modified; then, the obtained parameters are input into a pre-trained neural network model to generate preliminary and high-precision plasma control parameters; next, the operating parameters of the plasma device are controlled by a control unit to modify the material; finally, the state parameters of the material are collected in real time during the modification process, and these real-time state parameters are used as feedback input to the neural network model to dynamically update the plasma control parameters. This invention enables precise and adaptive control of plasma material modification to address nonlinear phase transitions in microstructures.

The present invention relates to methods of generating one or more transgenic cells using circular single stranded DNA (CiSSD) as a donor template, as well as target genome modification. The methods include transferring one or more DNA polynucleotides into a cell for site-specific nuclease-mediated DNA repair, and selecting one or more cells into which the transferred DNA is introduced in the genome of the cell.

The invention belongs to the technical field of biological medicines, and particularly relates to a myocardial targeted engineered extracellular vesicle as well as a preparation method and application thereof. The invention provides an engineered extracellular vesicle. The engineered extracellular vesicle is an engineered extracellular vesicle subjected to overexpression modification of gap junction protein alpha 1; the amino acid sequence of the gap connecting protein alpha 1 is shown as SEQ ID NO. 1. The engineered extracellular vesicles are modified by expression gap connecting protein alpha1, and compared with a control, the engineered extracellular vesicles modified by the gap connecting protein alpha1 are more ingested by myocardial cells, have higher heart targeting performance and can provide targeting drug carriers for patients with heart diseases. The invention provides a new drug research and development approach and means for clinical treatment of heart failure or myocardial injury, also provides a heart targeted modification tool for industrial research and development and basic scientific research users, and has very important medicinal value.