83 results about "Targeted Modification" patented technology

The invention relates to the technical field of biomedical engineering and drug delivery, and particularly discloses a novel targeted drug delivery system based on rose fruit extracellular vesicles, which comprises the following steps: preparing rose fruit source extracellular vesicles; preparing a medicine carrying vesicle; preparing targeted modified drug-loaded vesicles; according to the application disclosed by the invention, a stem related pathway is specifically down-regulated through the components of the rose fruits, and meanwhile, conventional tumor cells are killed by using adriamycin, so that a complementary treatment mechanism is formed, and the tumor recurrence risk is fundamentally reduced. The phenylboronic acid group of DSPE-PEG-PBA is used for specifically recognizing sialic acid over-expressed on the surface of a tumor cell, so that the enrichment of the medicine in tumor tissues is remarkably improved, and meanwhile, the distribution of normal tissues is reduced. By means of the natural biocompatibility and low immunogenicity of the RHNVs and in combination with the long circulation characteristic of a PEG chain, the delivery efficiency is guaranteed, the systemic toxicity is reduced to the maximum extent, and particularly the cardiotoxicity and myelosuppression of adriamycin are relieved.

The application discloses a method for producing deoxythymidine triphosphate by using a recombinant strain, and comprises the following steps: step 1: using a CRISPR / Cas9-mediated gene editing method to perform targeted modification on an E. coli genome, and the targeted modification comprises the following contents: a. knocking out a nucleoside triphosphate pyrophosphatase gene (mazG / yhdE) to block a path of degrading dTTP into dTMP; b. knocking out a nucleotide nucleosidase gene (ppnN) to block a path of degrading dTMP into thymine; and c. knocking out a nucleotidase gene (yfbR / yfdR / ushA / umpH / umpG / yjjG) to block a path of degrading dTMP into dT. The application provides a method for producing deoxythymidine triphosphate by using a recombinant strain, and the conversion rate of the method reaches 80% to 90%, and after ion exchange chromatography purification, the purity can reach more than 99%.

This invention relates to the field of metal material strengthening technology, specifically a method and system for enhancing the wear resistance of gate valves based on surface modification. The method includes: receiving a gate valve strengthening command; determining a target gate valve based on the command; identifying a set of target modification regions based on the target gate valve; performing surface pretreatment on multiple target modification regions within the target modification region set to obtain multiple pretreated modification regions; setting multiple powder mass ratios; constructing multiple laser cladding parameters; obtaining multiple cladding samples based on the laser cladding parameters and powder mass ratios; performing cladding performance testing on the multiple cladding samples to obtain multiple performance scores; determining a target cladding parameter combination based on the performance scores; and performing laser cladding on the multiple pretreated modification regions using the target cladding parameter combination to obtain a wear-resistant strengthened gate valve. This invention can improve the wear resistance of gate valves and extend their service life.

A duchenne muscular dystrophy-related exonic splicing enhancer, sgRNA and gene editing tool can be applied in the preparation of drugs for treating duchenne muscular dystrophy. The gene editing tool designed on the basis of cytosine deaminase AID mutants and Cas9 mutants can perform site-specific modification on a mammalian genome by using an adeno-associated virus (AAV) as a vector. By optimizing an encoding nucleic acid sequence and an element composition structure of the editing tool, site-specific targeted modification of mammalian genetic material DNA can be efficiently achieved; and by performing targeted genetic manipulation on the nucleic acid sequence carrying disease mutations, a pathogenic mutation cannot be retained in a mature protein amino acid sequence or the pathogenic mutation cannot perform its function, so that the purpose of treating various gene mutation type genetic rare diseases is achieved, and the advantages of high efficiency, safety and stability are achieved.

The invention relates to the field of computer data processing, and discloses a data integration method, device and equipment, and the method comprises the steps: generating equipment identification information corresponding to a plurality of pieces of computer equipment associated with a target user, and building an equipment information list; and if the equipment information of the current computer equipment is matched with the equipment information list, calling real-time modification data of the current computer equipment, carrying out modification action identification on the real-time modification data, obtaining target modification data corresponding to the target user, and carrying out data integration and updating on cloud storage data of the target user. According to the data integration method, device and equipment of the computer equipment, the computer equipment and the operation user generating modified data are synchronously recognized in the data integration process, the pertinence and integration efficiency of the data are effectively improved, and meanwhile, the user can conveniently, rapidly and accurately inquire the needed data.

The invention relates to the technical field of fusion membrane nano preparations, and provides a nano preparation capable of being used for cerebral arterial thrombosis gene drug targeted delivery, which comprises lipid-calcium phosphate nanoparticles, which are composed of DOPA modified calcium phosphate cores and lipidosome and are used for providing positive charges to obtain efficient gene loading efficiency and proton sponge effect, the intracellular efficient delivery and release of the gene are realized; and the exosome membrane is prepared from a cell culture solution extracted from bone marrow-derived mesenchymal stem cells and is used for improving the transfection efficiency of the carried medicine. According to the present invention, by using the process characteristic that the inner layer phospholipid and the outer layer phospholipid are not synchronously prepared during the LCP preparation process, the biological membrane of the exosome and the LCP outer layer phospholipid are fused during the LCP preparation of the outer layer phospholipid so as to construct the fusion membrane preparation having the characteristics of the biological membrane and capable of carrying out AMD3100 targeting modification, such that the advantages of the biological membrane and the AMD3100 in the targeting delivery system are combined so as to achieve the target delivery effect; and the bottleneck of drug delivery for brain diseases is solved.

The invention discloses a modified exosome as well as a preparation method and application thereof, and relates to the technical field of biological medicines, CD44 targeted modification and composite active ingredients are synergistic to realize efficient repair of burn wounds and sensitive skin, and the modified exosome can be specifically enriched on the burn wounds or damaged sensitive skin parts through CD44 targeted peptide fragment coupling, so that the effect of repairing the burn wounds and the sensitive skin is achieved. The defects that a traditional repairing product is poor in targeting property and low in active ingredient utilization rate are overcome, the loaded dipotassium glycyrrhizinate, glutathione and FGF form a synergistic effect, wound healing and skin barrier repairing can be promoted through the FGF, the dipotassium glycyrrhizinate and the glutathione can be used for strongly resisting inflammation and oxidation, release of sensitive skin inflammatory factors is reduced, and the repairing effect is achieved. By matching with nitrogen protection incubation and a pulse ultrasonic loading process, the stability of active ingredients is greatly improved, finally, the repair effects that the healing process of burn wounds is remarkably accelerated, and sensitive skin percutaneous moisture loss is obviously reduced are achieved, and safety and effectiveness are both considered.

The application discloses a kind of targeted drug-loaded nanoparticles response release performance detection method, specifically related to targeted nano-drug detection field, for solving the problem that only according to single release curve in the release performance detection of existing targeted drug-loaded nanoparticles, it is difficult to distinguish ordinary environment early leakage, insufficient release of channel plugging and stimulation response release. By generating a structure reference record containing initial drug loading, targeting modification amount and channel plugging state, and dividing the homologous sample into unmodified drug-loaded control group, targeted drug-loaded detection group and targeted modification blank correction group, and then combining the non-stimulation leakage curve, the stimulation switching release curve and the corrected release curve, the effect of distinguishing early leakage, stimulation response release and channel plugging effect is achieved, so that the detection result more accurately reflects the plugging leakage reduction ability and stimulation release ability of targeted drug-loaded nanoparticles.

The invention discloses a nano-drug for promoting cupric death of tumor cells and a preparation method of the nano-drug. The nano-drug comprises hyaluronic acid; a dopamine; copper ions; a diethyldithiocarbamic acid-copper conjugate; and a method for preparing the same. A peptide fragment with a targeting function; modifying a thiol ligand; the preparation method comprises the following steps: preparing a diethyl dithiocarbamic acid-copper conjugate, forming a nano-structure inner core, wrapping the conjugate, forming a copper ion protection layer, and purifying and carrying out targeted modification on a nano-drug. The preparation method comprises the following steps of: preparing the diethyl dithiocarbamic acid-copper conjugate, forming a nano-structure inner core, wrapping the conjugate, forming a copper ion protection layer, and carrying out targeted modification on the nano-drug. The copper ions are further fixed through the coordination effect of dopamine and the copper ions, wrapping of the diethyl dithiocarbamic acid-copper conjugate and the thiol-copper coordination effect of the modified thiol ligands, so that a double-coordination mechanism is formed, the loading capacity of the copper ions in the nano-drug is remarkably improved, and the stability of the nano-drug is improved. The copper death of tumor cells can be more effectively triggered, the copper ion loading capacity can be improved, and a material basis is provided for the efficient copper death effect.

The invention provides a retina photosensitive neuronal lesion zebrafish model and a construction method thereof, and relates to the technical field of gene engineering. Wherein the sgRNA comprises a guide sequence which can specifically recognize and be combined with a nucleotide sequence shown as SEQ ID NO.4 in an elovl2 gene. The high specificity of the sgRNA ensures the precise targeted modification of the elovl2 gene, and obviously reduces the off-target risk. The genetic background of the constructed zebrafish model is clear, and the retinopathy phenotype can be reliably attributed to the lovl2 gene mutation, so that a stable biological tool which is highly similar to human diseases is provided for related disease research and drug screening.

The invention discloses a precise editing method of a gamma-PGA high-yield strain based on CRISPR-Cas9n, and relates to the technical field of high-yield strains. According to the invention, a CRISPR-Cas9n double-nickase system is adopted, and adjacent DNA nicks are generated through the synergistic effect of double sgRNA, so that the non-specific cutting risk is greatly reduced; the double sgRNA targeting design is combined with a homologous recombination template, so that directional editing of key genes is realized, the homologous recombination efficiency reaches 75 + / -5%, and target modification is ensured to be stably integrated into a genome; by virtue of a PEG-mediated protoplast conversion method, the limitation of low efficiency of traditional electrotransfection is overcome, and the CRISPR carrier is rapidly removed in combination with a temperature-sensitive plasmid elimination system, so that cytotoxicity caused by continuous expression is avoided; under optimized fermentation conditions, the gamma-PGA yield of the edited strain reaches 15 + / -2g / L and is increased by 200% compared with that of the original strain.

The disclosure describes novel systems, methods, and compositions for the manipulation of nucleic acids in a targeted fashion. The disclosure describes non-naturally occurring, engineered CRISPR-Cas systems, components, and methods for targeted modification of nucleic acids such as DNA. Each system includes one or more protein components and one or more nucleic acid components that together target nucleic acids.

The invention relates to the technical field of biological medicine, and particularly discloses a lipidosome targeting SELENOP + macrophages, a preparation method of the lipidosome and application of the lipidosome in lung squamous cell carcinoma treatment. According to the lipidosome, an SELENOP antibody is used as a targeted modification molecule, si-SELENOP and s-ABCA1 / G1 are loaded, and accurate delivery of siRNA in target cells is achieved through specific binding of the antibody and a SELENOP receptor on the surface of a SELENOP + macrophage. The targeting liposome prepared by the invention can specifically inhibit cholesterol efflux pathways of SELENOP + macrophages, reverse tumor phenotypic polarization, remodel a tumor immune microenvironment and recover anti-tumor activity of immune cells such as CD8 + T cells, NK cells and the like. When the liposome is independently used or combined with a PD-1 antibody, the growth and metastasis of the lung squamous cell carcinoma can be remarkably inhibited, the treatment effect is improved, a brand new metabolism-immunity synergistic targeted treatment strategy is provided for the lung squamous cell carcinoma, and the liposome has a good clinical application prospect.

The invention belongs to the technical field of genetic engineering and plant genetic breeding, and particularly relates to a gene for regulating and controlling the cold tolerance of rice in the seedling stage and application of the gene. In order to excavate the gene for regulating and controlling the cold resistance of the rice, the nucleotide sequence of the rice gene OsCYP75B3 is mutated by virtue of a CRISPR / Cas9 genome targeted modification technology, and an experimental result shows that the cold resistance of the mutated rice in the seedling stage is remarkably reduced, so that the gene OsCYP75B3 is proved to negatively regulate and control the cold resistance of the rice in the seedling stage; the functional gene for regulating and controlling the cold resistance of the rice in the seedling stage is determined for the first time, and an important reference is provided for analyzing a molecular mechanism of the cold resistance of the rice in the seedling stage; based on the discovery, it is expected that the expression level of the gene OsCYP75B3 is improved through an overexpression technology, the gene OsCYP75B3 is applied to rice genetic engineering breeding, the cold resistance of rice is finally enhanced, and a key technical support is provided for guaranteeing grain safety.

This application relates to the field of game and animation production technology, and in particular to a method for modifying the appearance of animated characters, an electronic device, and a storage medium. This application first identifies the target driving bone from the skeletons of the target character model and adds a modification control component to the target driving bone; it then identifies the target modification controlled object associated with the target driving bone from a pre-set candidate control list; it captures bone pose transformation commands through the modification control component and determines the initial pose data, target pose data, and process pose data of the target character model based on the bone pose transformation commands, and then performs pose distance analysis to obtain a pose distance matrix; it performs radial basis function interpolation calculation based on the pose distance matrix to determine the corresponding modification weights for the process pose data; and finally, it performs character modification on the target modification controlled object to obtain the target animation of the modified character model. This method improves visual expressiveness while ensuring animation production efficiency.

A neural network-guided method for precise control of material modification, relating to the field of material modification technology, addresses the problem of uneven surface modification caused by the inability of existing plasma modification techniques to precisely control plasma parameters for nonlinear phase transitions in microstructures. This invention first obtains the initial state parameters and target modification parameters of the material to be modified; then, the obtained parameters are input into a pre-trained neural network model to generate preliminary and high-precision plasma control parameters; next, the operating parameters of the plasma device are controlled by a control unit to modify the material; finally, the state parameters of the material are collected in real time during the modification process, and these real-time state parameters are used as feedback input to the neural network model to dynamically update the plasma control parameters. This invention enables precise and adaptive control of plasma material modification to address nonlinear phase transitions in microstructures.

The present invention relates to methods of generating one or more transgenic cells using circular single stranded DNA (CiSSD) as a donor template, as well as target genome modification. The methods include transferring one or more DNA polynucleotides into a cell for site-specific nuclease-mediated DNA repair, and selecting one or more cells into which the transferred DNA is introduced in the genome of the cell.

The invention belongs to the technical field of biological medicines, and particularly relates to a myocardial targeted engineered extracellular vesicle as well as a preparation method and application thereof. The invention provides an engineered extracellular vesicle. The engineered extracellular vesicle is an engineered extracellular vesicle subjected to overexpression modification of gap junction protein alpha 1; the amino acid sequence of the gap connecting protein alpha 1 is shown as SEQ ID NO. 1. The engineered extracellular vesicles are modified by expression gap connecting protein alpha1, and compared with a control, the engineered extracellular vesicles modified by the gap connecting protein alpha1 are more ingested by myocardial cells, have higher heart targeting performance and can provide targeting drug carriers for patients with heart diseases. The invention provides a new drug research and development approach and means for clinical treatment of heart failure or myocardial injury, also provides a heart targeted modification tool for industrial research and development and basic scientific research users, and has very important medicinal value.

The invention provides an object modification method and device, electronic equipment and a storage medium, and relates to the technical field of large models, in particular to the technical field of artificial intelligence such as natural language processing. Determining target abnormal request ends with abnormal return quantity from the candidate object request end set according to the return object quantity; obtaining a target filtering strategy corresponding to the target exception request end, and determining a target modification strategy corresponding to the filtering strategy; and based on the target modification strategy, obtaining a target return object of the target exception request end, which is not filtered out by the target filtering strategy.

The invention discloses a method for improving the expression intensity of a bacillus subtilis self-induced expression system, and belongs to the technical field of genetic engineering and microbial engineering. According to the bacillus subtilis recombinant bacteria disclosed by the invention, pBLSI4310SP35UP3A containing Vibrio fischeri LuxI / R type QS is taken as a carrier, bacillus subtilis WB600 is taken as an expression host, and raw starch alpha-amylase derived from marine bacillus pincare sp.ZY is taken as a target protein. The activity of the bacillus subtilis recombinant extracellular AmyZ1 obtained by transforming a-35 region of a response module promoter RPlug < x > IR6 is 435 U / mL, which is 1.47 times of that of a control group, and the activity of the bacillus subtilis recombinant extracellular AmyZ1 is further improved to 926 U / mL, which is 3.14 times of that of the original control group, by directionally transforming an amino acid sequence of LuxR in the induction module, so that the activity of the bacillus subtilis recombinant extracellular AmyZ1 is improved to 926 U / mL, which is 3.14 times of that of the original control group. The self-induction expression intensity of the Vibrio fischeri LuxI / R type QS in the bacillus subtilis is obviously improved, and the self-induction expression intensity of the Vibrio fischeri LuxI / R type QS in the bacillus subtilis is obviously improved.

The invention discloses a pet stem cell exosome and drug co-delivery system and a delivery method. The system comprises an exosome acquisition module, a drug loading module, a targeted modification module, a quality control module and a finished product output and delivery module, the exosome acquisition module is used for extracting exosomes from the pet stem cells by using a differential centrifugation method; the drug loading module is used for efficiently and stably loading different types of drugs into the exosome; the targeted modification module is used for providing a targeted ligand for the finished product output and delivery module and realizing efficient combination of the targeted ligand and the exosome; the quality control module is used for strictly detecting the exosomes and removing unqualified products; the finished product output and delivery module converts the qualified targeting ligand into a clinically available preparation, and selects an adaptive administration mode to ensure that the drug smoothly enters the pet body, the rejection reaction caused by a heterologous vector is avoided, the drug can be naturally swallowed and degraded by target cells after entering the body, and the diseased tissue can be accurately positioned.

Provided are a docetaxel adiposome formulation, a preparation method therefor, and use thereof. The docetaxel adiposome formulation comprises a monomolecular phospholipid membrane and docetaxel and a neutral lipid that are wrapped in the monomolecular phospholipid membrane. Targeted modification is further carried out on the surface of the docetaxel adiposome. The adiposome formulation has good bioavailability and safety, and the anti-cancer effect of the formulation is significantly superior to that of a clinically used docetaxel injection.

To provide novel CRISPR DNA targeting enzymes and systems.SOLUTION: The disclosure describes novel systems, methods, and compositions for the manipulation of nucleic acids in a targeted fashion. The disclosure describes non-naturally occurring, engineered CRISPR systems, components, and methods for targeted modification of nucleic acids. Each system includes one or more protein components and one or more nucleic acid components that together target nucleic acids.SELECTED DRAWING: None

The application provides an interface configuration processing method and device, computer equipment and a computer readable storage medium. The interface configuration processing method comprises: in response to a touch operation of an interface management control in an operation page of a data service platform, displaying an interface management interface; obtaining a target modification instruction of a new editing function interface of the interface management interface; generating a temporary variable assignment statement of the new editing function interface according to variable editing information carried by the target modification instruction, the temporary variable assignment statement being used to assign a return value of an existing function interface to a variable of the new editing function interface; obtaining an edited interface code of the new editing function interface according to the temporary variable assignment statement and the edited interface code of the new editing function interface; and updating an interface configuration code set of the data service platform based on the edited interface code. The application can improve the reuse degree of the overall interface, improve the reuse degree of the overall interface, and reduce the time of repeated development.

The invention provides a cycloidal gear crowned shape modification method for eliminating a speed reducer gap and jittering, which comprises the following steps of: S1, determining a target shape modification working surface of a cycloidal gear to be modified between a tooth root working starting point and a tooth crest working ending point; s2, standard tooth profile data and preset drum shape modification amount data of the target shape modification working face are obtained; s3, a combined drum shape modification function is constructed, wherein the combined drum shape modification function is used for describing a continuous and non-constant drum shape quantity change rule at different positions in the tooth height direction of the working face; and S4, according to the combined drum shape modification function, a numerical control machining instruction is generated so as to machine the target shape modification working face, and a shape modification tooth face with the combined drum shape characteristic is formed. Compared with the prior art, the combined drum shape modification method is adopted, so that the technical problems of large backlash, large rotation error, low transmission precision and poor whole machine rigidity caused by partial backlash easily generated in a traditional shape modification mode are solved.

The application belongs to the technical field of biological medicine, and discloses a kind of high-efficiency vascular endothelial cell targeting vesicle system, which is prepared by hemangioma stem cell-derived exosome HemSCs-Exos and bone marrow mesenchymal stem cell-derived apoptotic body BMSCs-ABs, and the application further discloses a preparation method of the vesicle system and its application in promoting endothelial cell proliferation and migration. Unlike existing targeted modification, the ingenious targeting relationship between BMSCs-ABs and endothelial cells in the application belongs to the combination of non-special modification, which is simple and efficient. The fusion between double EVs has the characteristics of "1+1>2", which not only retains the therapeutic components of HemSCs-Exos and BMSCs-ABs, but also has a significant cell-specific distribution after fusion. The fusion method is simple and stable.

The disclosure describes novel systems, methods, and compositions for the manipulation of nucleic acids in a targeted fashion. The disclosure describes non-naturally occurring, engineered CRISPR-Cas systems, components, and methods for targeted modification of nucleic acids such as DNA. Each system includes one or more protein components and one or more nucleic acid components that together target nucleic acids.

The application provides a virtual vehicle modification method and system based on a virtual space, which comprises the following steps: importing an initial vehicle model and a modification part model into a virtual space to obtain an initial vehicle model and a modification part model; performing contact identification based on the modification part model to obtain a contact matrix, and performing collision information acquisition based on a collision detection model to obtain a collision matrix; outputting a modification sequence of the modification part model based on the contact matrix and the collision matrix to obtain a target modification sequence; performing sequence parallel optimization on the target modification sequence to obtain a final modification sequence; and assembling the modification part model to the initial vehicle model based on the final modification sequence to complete the virtual modification of the vehicle. The application can avoid falling into a local optimum condition, significantly improve the speed of sequence planning, improve the accuracy of sequence output, effectively shorten the overall assembly time and greatly improve the assembly efficiency.

The invention belongs to the technical field of nano drug delivery systems, and particularly relates to a bone morphogenetic protein loaded nano drug delivery system and application in postoperative healing, and the bone morphogenetic protein loaded nano drug delivery system comprises a composite carrier, a targeted modification layer and loaded bone morphogenetic protein; the composite carrier is formed by compounding an inorganic nano material and a biodegradable polymer, the targeted modification layer is a bone injury part specific recognition molecule, and the nano drug-loading system has an intelligent release mechanism responding to a bone healing microenvironment and can be applied to orthopedic postoperative healing.