8 results about "Targeted Modification" patented technology

This invention discloses an engineered bacterium that efficiently catalyzes the synthesis of N-acetyl-natamycin and its applications, belonging to the fields of biocatalysis and enzyme engineering technology. This invention also provides an N-acetyltransferase mutant. Mt GNAT W289A / S121G This mutant, obtained through targeted modification, can catalyze the synthesis of N-acetyl-natamycin using natamycin as a substrate and acetyl-CoA as an acetyl donor. After fed-batch fermentation, the wet cell yield reached 124.8 g / L. In a 1 L reaction system using a 30 g / L catalyst, the N-acetyl-natamycin yield reached 31.2 g / L, with a conversion rate of 90.2%. The wet cells obtained from 1 L of fermentation could catalyze four batches of reactions, yielding a cumulative total of 122.3 g of N-acetyl-natamycin. This invention features mild reaction conditions, high selectivity, and few byproducts, making it suitable for the efficient preparation of N-acetyl-natamycin.

A neural network-guided method for precise control of material modification, relating to the field of material modification technology, addresses the problem of uneven surface modification caused by the inability of existing plasma modification techniques to precisely control plasma parameters for nonlinear phase transitions in microstructures. This invention first obtains the initial state parameters and target modification parameters of the material to be modified; then, the obtained parameters are input into a pre-trained neural network model to generate preliminary and high-precision plasma control parameters; next, the operating parameters of the plasma device are controlled by a control unit to modify the material; finally, the state parameters of the material are collected in real time during the modification process, and these real-time state parameters are used as feedback input to the neural network model to dynamically update the plasma control parameters. This invention enables precise and adaptive control of plasma material modification to address nonlinear phase transitions in microstructures.

The application provides an interface configuration processing method and device, computer equipment and a computer readable storage medium. The interface configuration processing method comprises: in response to a touch operation of an interface management control in an operation page of a data service platform, displaying an interface management interface; obtaining a target modification instruction of a new editing function interface of the interface management interface; generating a temporary variable assignment statement of the new editing function interface according to variable editing information carried by the target modification instruction, the temporary variable assignment statement being used to assign a return value of an existing function interface to a variable of the new editing function interface; obtaining an edited interface code of the new editing function interface according to the temporary variable assignment statement and the edited interface code of the new editing function interface; and updating an interface configuration code set of the data service platform based on the edited interface code. The application can improve the reuse degree of the overall interface, improve the reuse degree of the overall interface, and reduce the time of repeated development.

This invention discloses a targeted anti-aging microemulsion skincare formulation containing proanthocyanidin C1 and its preparation method, comprising the following components by weight percentage: oil phase: 12%–22%; nonionic surfactant: 18%–28%; co-surfactant: 6%–12%; proanthocyanidin C1: 0.8%–4.5%; marine collagen oligopeptides: 2%–7%; targeted modification component: 0.2%–0.8%; deionized water: balance; the formulation is an oil-in-water microemulsion with a particle size of 10–50 nm and a polydispersity index ≤0.2; the purity of the proanthocyanidin C1 is ≥95%; this invention relates to the field of anti-aging technology. This targeted anti-aging microemulsion skincare formulation containing proanthocyanidin C1 and its preparation method involve compounding an oil phase, nonionic surfactant, and co-surfactant in a specific ratio, combined with a two-stage high-pressure microfluidic homogenization process, to prepare an oil-in-water nanoscale microemulsion with a particle size strictly controlled within the range of 10–40 nm and a polydispersity index ≤0.15. The system is uniform and delicate, without stratification, flocculation, or oil droplet aggregation.

The embodiment of the invention provides a program development method and device, a computer program product and a storage medium. According to the method, various program templates with complete functions are provided for a user, and on the basis, the user can select a proper target program template when the user needs to carry out program development. A user can be supported to submit program development requirements according to a natural language, a target modification object concerned by the user is automatically positioned in a target program template, and modification content meeting the program development requirements is generated for the target modification object by utilizing a large model; on the basis, the target modification object can be modified in the target program template according to the modification content generated by the large model, so that a program product required by a user is generated. Therefore, the program development threshold can be effectively reduced, and the program development quality can be effectively guaranteed.

This application discloses a method for targeted modification to improve the activity of halohydrin dehalogenase, belonging to the field of bioengineering technology. The halohydrin dehalogenase mutant obtained by this application is produced by single-point mutation or combination mutation of alanine at position 60 and histidine at position 179 in the sequence shown in SEQ ID NO.1. Compared with the wild-type halohydrin dehalogenase, the mutant obtained in this application shows significantly improved enzyme activity and dehalogenation efficiency in the catalytic preparation of 1-chloro-2-propanol and 2-chloro-1-ethanol. The mutant enzyme activity is increased by 1.78 times, and the dehalogenation efficiency is increased by 77.8%, showing good prospects for industrial application.

The present invention provides a method for plant genome modification, preferably for the targeted modification of at least one genomic target sequence, for obtaining at least one modified cell, wherein modification of a cell is achieved by providing a genome modification or editing system together with at least one regeneration booster, or a combination of regeneration boosters, which is / are transiently active in the cell and / or with at least one epigenetically regulating chemical. Preferably, the effector molecules are introduced by means of particle bombardment. Furthermore, the modified plant cell is regenerated to obtain a plant, which inherits the modification to its progeny. In addition, methods, tools, constructs and strategies are provided to effectively modify the genome of a plant cell or at least one genomic target site in a plant cell, to obtain said modified cell and to regenerate a plant tissue, organ, plant or seed from the modified cell. Finally, the present invention also relates to an improved method of regenerating a plant tissue, organ or a plant from a single plant cell.

The application discloses a terrain special effect processing method and device, a storage medium, equipment and a program product. The method comprises the following steps: acquiring terrain height field data of a target model, determining terrain features of the target model based on the terrain height field data; based on the terrain features, determining target region information of the target model that meets preset terrain features, wherein the target region information comprises spatial feature information and modification parameter information of the target region; based on the target region information, selecting a target modification tool from preset terrain modification tools, and controlling the target modification tool to modify the terrain of the target region, so that the terrain of the target region matches the terrain of the target modification tool. The target modification tool can make the terrain form, terrain height and terrain material of the target region consistent with the preset details of the target modification tool, and the modification can be quickly completed through the target modification tool, thereby improving the modification efficiency. The terrain features quantize the terrain form (such as high curvature for ridges and high slope for steep slopes), ensure that the terrain feature recognition conforms to the natural law, and avoid the deviation of artificial judgment.