280 results about "Lung alveolus" patented technology

The invention provides a methylation detection combined marker based on free DNA (cfDNA) in pulmonary alveolar lavage fluid (BAL) and application of the methylation detection combined marker. The methylation combination marker optimizes methylation detection targets in early screening of lung cancer, reduces detection difficulty and cost, and has high diagnosis accuracy, sensitivity and specificity.

A facemask and accessories which overcomes the adverse effects and limitations of current designs by moving the maxilla and mandible in forward direction, which assists in correcting maxillary and mandibular skeletal and dentoalveolar deficiency and help expand the upper airway by advancing the mandible. This is accomplished by a design that is supported only by forehead and produces forces in front of maxilla and mandible. The extra-oral facebow design is characterized by providing two independent force adjustments, one above the forehead and the other one in front of the face by attachments to the upper and lower jaw bones and teeth. In particular, the two independent adjustments are provided by a fully adjustable forehead pad and bilateral adjustable bows on opposite sides of the facebow's extra-oral frame to provide protracting forces.

The invention relates to the technical field of medical data analysis, in particular to a sepsis prediction system based on deep learning. The system comprises a sepsis risk assessment module, a capillary barrier injury analysis module, a myocardial inhibition analysis module and a sepsis prediction module, and blood lactic acid concentration data of a patient are obtained through a lactic acid analyzer; acquiring oxygenation state data of a patient through a blood gas analyzer, and performing sepsis risk assessment according to the oxygenation state data of the patient and the blood lactic acid concentration data of the patient, so as to obtain sepsis risk data; obtaining a lung structure image through a high-resolution chest CT; performing alveolar feature extraction according to the lung structure image to obtain alveolar data; according to the pulmonary alveolar data, capillary barrier injury analysis is carried out, so that capillary barrier injury data is obtained; an echocardiogram is obtained by an echocardiograph. The sepsis prediction accuracy is improved based on a medical data analysis technology.

The invention relates to a method for carrying out pathogenic microorganism identification and gene variation state joint detection on a pulmonary alveolar lavage fluid sample based on high-throughput sequencing. The method comprises the following steps: extracting nucleic acid from the pulmonary alveolar lavage fluid sample; constructing a metagenome capture library, and constructing a lung cancer polygene library; carrying out final library pooling; performing high-throughput sequencing; and analyzing data. Compared with the prior art, the scheme provided by the invention is based on a technical path of'macro 'captured by metagenome and'target' of targeted high-throughput sequencing; after the constructed final library is subjected to high-throughput sequencing and data analysis, index output (pathogenic microorganism identification, host chromosome copy number variation, gene mutation, gene fusion, gene copy number variation and methylation state) of multiple biomarkers can be met in one-time detection.

The utility model discloses a pulmonary alveolar lavage device which comprises a suction catheter and an ultrasonic probe, wherein the suction catheter is used for entering a bronchus through an insertion tube of an endoscope for pulmonary alveolar lavage, and the ultrasonic probe is used for entering the bronchus through the suction catheter. The suction catheter comprises a non-transparent flexible section located at the far end and a transparent section communicated with the near end of the flexible section, and the hardness of the transparent section is larger than that of the flexible section. By using the alveolar lavage device, the shape of the bronchus into which the suction catheter enters and the runner condition of the suction catheter can be observed in real time, and the suction catheter can be prevented from being blocked.

The invention belongs to the technical field of microorganisms, and particularly relates to application of bifidobacterium longum in preparation of products for prevention, treatment and / or adjuvant treatment of pneumonia. The preservation number of the bifidobacterium longum is GDMCC No: 65969, and the bifidobacterium longum can improve the body weight decrease trend of a pneumonia mouse and reduce the lung visceral organ index of LPS increase; the content of pro-inflammatory factors such as IL-1beta, TNF-alpha and IL-6 in serum is remarkably reduced, mRNA expression of inflammation-related genes such as COX-2, IFN-gamma and TNF-alpha in lung tissue is reduced, and dose dependence is achieved; meanwhile, alveolar structure damage and inflammatory cell infiltration of mouse lung tissue are relieved, and lung tissue pathological damage is repaired. The strain can be used as a core component for developing pneumonia prevention or adjuvant therapy related products, provides a novel microbial intervention means for pneumonia prevention and treatment, and has important application value.

The invention provides a small animal breathing machine and a control method, the small animal breathing machine comprises an air pump set, a breathing control valve, a breathing machine controller and a touch screen displayer, the air pump set comprises a plurality of air pump units arranged in parallel and a silica gel pipeline, the multiple air pump units are connected in parallel, and the air pump units with different flows can be freely combined according to different animal and tidal volume requirements; the air flow of each air pump unit is accurately distributed, meanwhile, the high-precision and large-flow requirements of the breathing machine are met, a three-way pipeline and a valve element are arranged in the breathing control valve, and the pulmonary alveolar connecting end, the three-way pipeline and the air outlet pipeline form an air inlet path; the alveolar connecting end, the three-way pipeline and the waste collecting end form an exhaust gas path, and the valve element can switch the direction of an internal gas path of the three-way pipeline. The breathing control valve controls the airflow direction only through the action of the valve element, so that the air pump unit can operate continuously, frequent starting and stopping are not needed, the service life of the air pump unit is remarkably prolonged, and the response speed is increased.

The invention belongs to the field of biological medicine, and particularly relates to an inhalable small molecule compound lung targeting delivery system and application thereof in radiation-induced lung injury protection. The invention develops a probiotic-drug symbiotic delivery system based on lactobacillus rhamnosus on the basis of finding that a small molecular compound with a molecular structure as shown in a formula (I) has excellent SOD and CAT enzyme activity and can prevent radiation-induced lung injury caused by radiotherapy for the first time. Wherein the small molecule compound is loaded on the surface of lactobacillus rhamnosus and is directly delivered to the alveolar cavity through aerosol inhalation, so that the targeting property and bioavailability of the small molecule compound at the RILI part can be enhanced. # imgabs0 # (I)

The invention provides an organ chip, a lung organ model, a construction method and a simulation method, the organ chip comprises a culture membrane and an actuating membrane, the organ chip is further provided with a first culture chamber, a second culture chamber, an actuating chamber and a perfusion channel, the culture membrane separates the first culture chamber and the second culture chamber, the perfusion channel is communicated with the first culture chamber, and the actuating chamber is communicated with the second culture chamber. A communication port of the perfusion channel and the first culture chamber faces the culture membrane, preferably directly faces the culture membrane, the perfusion channel is used for connecting an external air source, the actuating chamber separates the second culture chamber and the actuating chamber, and the actuating chamber can deform in response to a pressure change of the actuating chamber so as to conduct the pressure change between the actuating chamber and the second culture chamber; further, the culture membrane is deformed. The organ chip can more truly simulate the respiratory movement process of pulmonary alveoli and the multi-directional shear stress generated by gas to cells, and is beneficial to meeting the research requirements of respirator-related lung injury.

The invention provides a co-culture system of human alveolar epithelial cells and endothelial cells, which is used for researching interaction in an epithelial-endothelial barrier in lungs. It is indicated that infection with the highly pathogenic influenza A virus A / HK / 483 / 97 (H5N1) can significantly reduce alveolar fluid clearance (AFC) and increase protein permeability (APP). Administration of therapeutic extracellular vesicles (EV) after infection effectively restores AFC and APP, reduces viral replication, and enhances expression of antiviral genes. According to the invention, the treatment potential of EV is highlighted, and a valuable tool is provided for researching acute lung injury and improving a lung drug delivery technology.

The invention relates to a lung in-vitro model and a construction method and application thereof. The lung in-vitro model provided by the invention is good in stability and controllability and high in maturity, can better simulate functions and structural characteristics of pulmonary alveoli-capillary barriers, has the advantage of high simulation, and has a good application prospect in the aspects of disease simulation, drug detection, screening, evaluation, research and development and the like.

The invention belongs to the technical field of biological medicines, and particularly relates to application of small interfering RNA (Ribonucleic Acid) of a targeted Zyx gene. The invention provides siRNA (small interfering Ribonucleic Acid) of a targeted Zyx gene, and further provides application of the siRNA in prevention and treatment of fibrosis interstitial lung diseases. Experimental data prove that in a mouse bleomycin model, siRNA of a targeted Zyx gene can effectively improve the survival rate of a sick mouse, alveolar wall thickening, lung substantive thickening and inflammatory cell infiltration of a Zyx knock-down group are not obvious, and pathological damage caused by bleomycin is effectively relieved through knock-down of the Zyx gene; collagen fiber deposition of a Zyx knock-down group is reduced, and the transcription level of fibrosis-related genes is obviously reduced.

The invention belongs to the field of biological medicines, and discloses application of flufenidone in preparation of a medicine for treating pancreatic diseases. Researches find that the flufenidone can improve serum amylase and lipase levels of an acute pancreatitis model mouse, and can improve pancreatic tissue edema, necrosis and inflammatory cell infiltration of the acute pancreatitis model mouse; the traditional Chinese medicine composition can improve acute pancreatitis model mouse lung tissue alveolar interval thickening and inflammatory cell infiltration, and provides guarantee for application of the traditional Chinese medicine composition in preparation of drugs for treating pancreatic diseases.

A reagent kit, staining method, and application for combining dual immunohistochemistry with elastin fiber staining. The reagent kit includes a combination of primary antibodies, consisting of anti-CK7 and anti-CD34 monoclonal antibodies from different species, such as rabbit anti-human CK7 monoclonal antibody and mouse anti-human CD34 monoclonal antibody. By selecting CK7 and CD34 as the primary antibody combination, the growth pattern of tumor epithelium can be accurately indicated, and the proliferation and thickening of alveolar septal fibrous tissue caused by tumors can be clearly revealed, as opposed to structural changes of alveoli induced by other factors. Combined with independent Victoria blue staining, the elastin fibers of the walls of arteries and veins, as well as the pleura of the lungs, can be clearly visualized, aiding in the assessment of the integrity of elastin fibers on the lung surface and in determining the extent of tumor infiltration.

The invention provides a monitoring method, system and device based on a shared EIT wearable sensor and a medium, and the method comprises the steps: measuring the dynamic change of electrical impedance in a thoracic cavity through a sensor device attached to a chest region of a tested person, so as to collect signals related to pulmonary ventilation and blood flow; the collected signals related to lung ventilation and blood flow are subjected to signal processing, the pulmonary alveolar ventilation volume and the lung blood flow within unit time are calculated according to the signals so as to obtain the real-time ventilation / blood flow perfusion ratio, and regional analysis is carried out so that synchronous monitoring of the ventilation / blood flow perfusion ratios of different lung areas can be carried out. By sharing the EIT sensor array, synchronous monitoring of systemic circulation, pulmonary ventilation and the V / Q ratio is achieved, the number of used sensors is reduced, and the burden and medical cost of a patient are reduced. By combining an intelligent algorithm and a data fusion technology, comprehensive physiological information is provided, and a new solution is provided for personalized medical treatment and remote monitoring.

The invention relates to application of visceral odorobacter and outer membrane vesicles thereof in preparation of drugs for treating acute lung injury, and relates to the technical field of drugs. The modeling medicine lipopolysaccharide is applied to a mouse individual in a nasal dripping mode, the administration mode is that each mouse is administered once a day, and intragastric administration is carried out once a day after modeling is carried out for two days and lasts for 7 days. Experiments prove that visceral odorobacter and outer membrane vesicles of the visceral odorobacter reduce the number of M1 type alveolar macrophages by down-regulating proinflammatory factors IL-6, TNF-alpha and IL-1beta in lung tissues and inhibiting three proinflammatory factors IL-6, TNF-alpha and IL-1beta in serum, so that the effect of remarkably reversing acute lung injury is achieved. The visceral odor bacilli and the outer membrane vesicles thereof are used for preventing and treating the acute lung injury, and a new method and means are provided for clinically treating the acute lung injury.

The invention relates to the technical field of medical image processing, in particular to a lung vessel tree reconstruction method based on multi-scale watershed segmentation, which comprises the following steps: acquiring lung CT image data; based on the lung CT image data, executing image preprocessing operation to obtain preprocessed image data; based on the preprocessed image data, executing multi-scale analysis to obtain multi-scale feature data; based on the multi-scale feature data, executing a drainage basin segmentation algorithm to obtain a blood vessel segmentation result; based on the blood vessel segmentation result, executing a blood vessel tree reconstruction operation to obtain reconstructed lung blood vessel tree data; according to the method, through innovative combination of multi-scale analysis and drainage basin segmentation, comprehensive processing of blood vessels with different thicknesses is realized. Multi-scale analysis can capture blood vessel characteristics under different scales, and drainage basin segmentation can accurately position blood vessel boundaries, so that the method can process thick and large blood vessels such as main pulmonary artery and the like and capillary vessels around tiny pulmonary alveoli at the same time.

The invention relates to the technical field of medicines, in particular to application of lycium barbarum-derived extracellular vesicles in preparation of a medicine for treating acute kidney injury. The used extracellular vesicles derived from Chinese wolfberry fruits have a lipid bilayer membrane structure, are good in stability, free of remarkable toxicity, good in blood compatibility and high in-vivo safety after long-term intravenous injection, are used for preparing the medicine for treating the acute kidney injury, and can reduce apoptosis, inhibit rising of blood urea nitrogen and creatinine levels and relieve injury of kidney tissues; the invention further provides application of the extracellular vesicles derived from the Chinese wolfberry fruits in preparation of the medicine for treating the lung injury disease, the weight loss is relieved, lung tissue is protected, meanwhile, the pulmonary alveolar-capillary barrier can be repaired, and in addition, the medicine can be used for treating the lung injury disease. The invention also provides application of the extracellular vesicles derived from Chinese wolfberry fruits in preparation of nuclear transcription factor kappa B inhibitors and / or inhibition of apoptosis and / or repair of pulmonary alveolar capillary barriers.

The invention relates to the field of biology, in particular to a fluorescence and bar code pedigree tracking alveolar organ and a construction method and application thereof. The invention particularly aims at the problems of specific sorting of AT2 cells, culture of organoids and pedigree retracking in the disease process, and is used for simulating and researching the in-vitro pathological process of chronic obstructive pulmonary disease (COPD). The alveolar organ with fluorescence and bar code pedigree tracking is constructed and can track the fate transformation of alveolar type II cells (AT2) in a disease model, and the cell pedigree is further decoded, so that a new research basis is provided for early diagnosis and treatment of COPD (chronic obstructive pulmonary disease).

The invention discloses a lung organ chip based on a stretchable biological membrane and a preparation method of the lung organ chip, and belongs to the field of biomedicine and pharmacology. The chip comprises a top cell culture layer, a bottom vacuum negative pressure layer and a stretchable porous membrane layer, the top cell culture layer comprises a cell culture chamber, a fluid micro-channel and a fluid micro-channel outlet / inlet, and the cell culture chamber, the fluid micro-channel and the fluid micro-channel outlet / inlet are communicated with one another; the bottom vacuum negative-pressure layer comprises a vacuum negative-pressure chamber, a gas micro-channel and a gas micro-channel outlet / inlet, and the vacuum negative-pressure chamber, the gas micro-channel and the gas micro-channel outlet / inlet are communicated with one another; the stretchable porous film layer is a deformable single-layer elastic film, and a plurality of through round holes simulating the alveolar structure are formed in the middle of the stretchable porous film layer. The method has the characteristics of low cost, easiness in manufacturing, easiness in operation and the like, and application and popularization of the lung organ chip are facilitated.

The invention belongs to the technical field of organoid culture, and particularly relates to a pulmonary alveolar organoid with an endogenous vascular network and a construction method of the pulmonary alveolar organoid. Aiming at the problems that mesoderm and endoderm are difficult to induce at the same time, the operation process is long, the difficulty is high and the like when a vascularized alveolar organ is constructed by an existing method, the invention provides the construction method of the alveolar organ with the endogenous vascular network. The method comprises the following steps: simultaneously and directionally inducing and differentiating human induced pluripotent stem cells (hiPSC) into fixed endoderm and mesoderm, anterior intestine endoderm, abdominal side anterior intestine endoderm and lung progenitor cells by adopting a one-step method, and promoting endothelial cell proliferation in an endogenous induced differentiation process through a specific growth factor combination. A functional vascular network is formed; and vascularized alveolar organs are induced to be generated. According to the invention, a qi-blood barrier structure in which vascular endothelial cells and pulmonary epithelial cells are tightly connected is successfully reproduced, the gas exchange function of lung tissues can be simulated, and a new way is provided for construction of vascularized alveolar organs.

The invention belongs to the technical field of medicines, and particularly discloses application of binding molecules of actin binding protein LASP1 in prevention and treatment of acute respiratory distress syndrome (ARDS). According to the invention, the LASP1-loaded nanoparticles are constructed, so that the problems that the drug is difficult to penetrate and easy to remove and degrade and the ARDS is difficult to treat are solved. According to the invention, through intranasal instillation of the HA-DA / BP / LASP1 nanoparticles, the level of inflammatory factors can be reduced, the alveolar epithelial barrier structure and function can be repaired, and the death rate of ARDS model mice can be reduced.

The invention relates to a bronchoalveolar lavage fluid rapid detection method based on a fluorescent staining technology and a corresponding detection device, and aims to improve the detection efficiency of alveolar lavage and sampling screening. The detection device comprises an endoscope assembly, a lavage fluid supply module, a recovery module and a control module. Wherein the recovery module is internally provided with an assembly used for adding a fluorescent coloring agent into the recovery liquid, the fluorescent coloring agent is injected into the recovery liquid in the recovery process of the lavage fluid according to needs, and an electromagnetic drive stirring mechanism is combined, so that dynamic and uniform mixing of the dyeing liquid and the lavage fluid is achieved. The control module automatically adjusts the injection amount, the mixing speed and the stirring duration of the coloring agent according to preset parameters or real-time detection data, and the dyeing reaction time in the operation process is remarkably saved.

The invention belongs to the technical field of biological medicine manufacturing, and particularly provides a preparation method of an engineered exosome capable of loading mitochondria for treating pulmonary fibrosis and the engineered exosome. The method comprises the following steps: constructing fusion plasmids for expressing mitochondrial targeting peptide fragment-therapeutic protein genes, dissolving the fusion plasmids in non-enzyme ultrapure water, adding the fusion plasmids into a lipid nano-preparation, and fully mixing to obtain a nano-preparation containing the therapeutic protein genes; the nano preparation is delivered into alveolar epithelial cells and is efficiently transfected, so that the homeostatic balance of the injured alveolar epithelial cells in the development of pulmonary fibrosis is effectively regulated and controlled, the secretion of an exosome with a therapeutic effect is promoted, and the engineered exosome is further obtained through mannose modification. The exosome can effectively load mitochondria and sequentially and accurately target macrophage mitochondria on the basis of a mannose target head and a mitochondrial targeting peptide fragment, so that macrophage mitochondrial homeostatic balance is effectively regulated and controlled, the purpose of reverse treatment of pulmonary fibrosis is achieved, and the exosome has high clinical application value.

The application discloses a lung ventilation-perfusion imaging area image segmentation and quantitative analysis system and relates to the technical field of medical image analysis.The system comprises a data acquisition module, which acquires SPECT V / Q image data and case information corresponding to the SPECT V / Q image data, and generates a structured data set; a feature extraction module, which performs format conversion and multi-scale feature extraction on the SPECT V / Q image data in the structured data set, and generates a compressed feature map data set; a lung lobe segmentation module, which inputs the compressed feature map data set into a U-Net model, segments lung lobe areas, performs post-processing, and generates a lung lobe area mask image; and an image fusion module, which performs alpha blending fusion on the lung lobe area mask image and the SPECT V / Q image data, and generates a color fusion image.The application captures lung alveolus-to-lung lobe structure by fusing four branch features of a hollow pyramid network, and reduces GPU memory consumption.

The invention discloses a formula for reducing phlegm and resolving masses, and a preparation method and application of freeze-dried powder of the formula for reducing phlegm and resolving masses, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition inhibits triple negative breast cancer adhesion-metastasis by regulating an FAK / SRC pathway, and comprises the following components by weight: 10-20 g of poria cocos, 10-20 g of rhizoma bolbostemmae, 10-20 g of radix trichosanthis, 5-12 g of curcuma zedoary, 10-20 g of selfheal, 10-20 g of oyster (smashed and decocted first), and 5-12 g of uniflower swisscentaury root. In-vitro experiments show that HTSJF can inhibit TNBC cell invasion, migration and adhesion (P is less than 0.01) in a dose-dependent manner, and along with reduction of the phosphorylation level of an FAK / SRC pathway, it is prompted that HTSJF inhibits TNBC transfer by regulating the pathway. In-vivo experiments prove that the HTSJF can obviously inhibit the reduction of TNBC pulmonary metastasis focus (P is less than 0.05) and recover part of normal pulmonary alveolar structures, and the inhibition effect of the HTSJF on TNBC pulmonary metastasis is further proved.

The invention provides a compound based on aspergillus oryzae spores. The compound is composed of carbonized aspergillus oryzae spore microsphere surfaces and pores loaded with quercetin. The average porosity of the carbonized aspergillus oryzae spore microspheres is 25%-45%; the mass of the quercetin accounts for 30%-50% of the total mass of the compound. According to the compound, the solubility and bioavailability of quercetin are remarkably improved, efficient pulmonary alveolar deposition is guaranteed through the optimized aerodynamic diameter of the compound, and rapid intake of macrophages is guaranteed through the rough surface of the compound. An in-vivo and in-vitro ALI model verifies the safety of the compound QU-cASP prepared by the invention, and the compound QU-cASP has excellent effects in the aspects of targeted delivery, oxidative stress inhibition and macrophage polarization regulation. The invention also provides a method for preparing the compound, an application of the compound in preparation of a medicine for relieving acute lung injury, and an inhalable preparation taking the compound as an active ingredient.

The invention relates to the technical field of organoid culture, in particular to a pulmonary fibrosis organoid model and a construction method and application thereof. The pulmonary fibrosis organ-like model is obtained by co-culturing lentivirus stably transfected alveolar epithelial cells, human embryonic lung fibroblasts MRC-5 and macrophages. According to the invention, macrophages are innovatively introduced, a ternary co-culture system of lentivirus stably transfected alveolar epithelial cells, human embryonic lung fibroblasts MRC-5 and macrophages is constructed, and a key'epithelial injury-immune infiltration-fibroblast activation 'pathological triangle in a pulmonary fibrosis pathogenesis process can be more comprehensively simulated; the finally obtained pulmonary fibrosis organ-like model provides an efficient tool for pulmonary fibrosis mechanism research and research, development and screening of anti-pulmonary fibrosis drugs.

The present invention belongs to the field of biomedicine technology, and in particular to the application of Corynebacterium casei in the preparation of medicines for preventing and treating lung injury. The present invention constructs a mouse model of lung injury caused by radon inhalation and finds that after exposure to different radon levels, the mice lose weight, increase the lung coefficient, reduce the lung tidal volume, minute ventilation and maximum inspiratory flow, and their respiratory function is inhibited. The alveolar cavity of the mice is abnormally expanded, the lung septum is thickened and fractured, accompanied by more fibrin exudation, obvious inflammatory infiltration appears near the bronchus, congestion occurs in small blood vessels, and collagen deposition in lung tissue can lead to the occurrence of pulmonary fibrosis, causing lung injury. Oral administration of Corynebacterium casei can effectively inhibit the above-mentioned phenomenon. Therefore, Corynebacterium casei can be used for the prevention and treatment of lung injury, especially lung injury caused by radon exposure.

An opening is formed in the side wall of the front section of a reducing part of a Venturi tube, an air suction tube externally connected with an oxygen source is introduced into the opening, an air suction tube body is located in the Venturi tube, and an air suction tube outlet extends to the position of a patient interface; a valve block matched with the pipe diameter is arranged in the air suction pipe, the valve block is fixedly connected with a shaft rod of the adjusting rotating wheel, the valve block can be driven to rotate in the air suction pipe by rotating the adjusting rotating wheel, and therefore the opening degree of an air inlet channel of the air suction pipe is adjusted. According to the utility model, constant bias flow towards the interior of the lung is added in the whole breathing process, and meanwhile, the bias flow can be used for adjusting the flow, so that the oxygen saturation of a patient in the exercising process is in a stable state; in addition, the phenomena of alveolar collapse or pulmonary atelectasis aggravation caused by overlarge shearing force at the end of expiration of the patient can be reduced as much as possible.