The invention relates to an extract product capable of preventing oxidation, delaying aging, and nourishing heart and cerebral vessels, and a preparation method thereof. According to the preparation method, a grape extract and a mixed fruit powder are prepared firstly, and are mixed with yeast for fermentation, after metabolism of yeast, organic conversion of resveratrol in the grape extract, andanthocyanidin and a plurality of trace elements in the mixed fruit powder is realized, so that adsorption on nutrients is improved, and the biological value is increased. It is confirmed by animal experiments that the extract product is capable of prolonging mouse life time and rat loaded swimming time obviously, reducing blood urea nitrogen content, reducing blood lactic acid accumulation, and increasing hepatic glycogen content, possesses obvious anti-fatigue activity, is capable of increasing the activity of superoxide dismutase in rat serum, and accelerating malonaldehyde removing rate, and possesses obvious anti-oxidation activity. The extract product is prepared from pure natural materials, no chemical raw material is added, no toxic or side effect is caused by long term eating. Theextract product is suitable for fatigable people, people high in cholesterol content, and people with long term smoking and drinking habits.