Detecting reagent of urea nitrogen and detecting test paper of urea nitrogen

A detection reagent and detection test paper technology, which is applied in the field of urea nitrogen detection, can solve the problems of high material requirements, complex process, and the detection accuracy needs to be improved, and achieves strong anti-interference ability, eliminates interference, and ensures fast and relatively accurate detection. Effect

Active Publication Date: 2017-05-10
复星诊断科技(长沙)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of liquid-locking structure has a complicated process, requiring the liquid-locking parts to absorb liquid at a rate less than or equal to the liquid-absorbing speed of the reaction layer. The material requirements for the liquid-locking parts are relatively high, and a process is added to the test paper production process, making the production of test paper The process is more complicated
[0006] In addition, in the above existing technologies, the detection accuracy needs to be improved, and the detection is not fast enough

Method used

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  • Detecting reagent of urea nitrogen and detecting test paper of urea nitrogen
  • Detecting reagent of urea nitrogen and detecting test paper of urea nitrogen
  • Detecting reagent of urea nitrogen and detecting test paper of urea nitrogen

Examples

Experimental program
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Effect test

Embodiment 1

[0055] A kind of detection test paper 1 of urea nitrogen, its preparation method comprises the following steps:

[0056] Step 1: Paste double-sided adhesive tape 200 on the reactive base layer 100 made of PET material with a certain hardness, and punch through holes 110 for later use.

[0057] Step 2. Tear off the oil paper of the double-sided adhesive 200 on the reaction base layer 100, and paste the film material 320 on the double-sided adhesive tape 200 of the PET board. The film material 320 needs to completely cover the through hole 110 on the PET substrate.

[0058] Step 3: First prepare the detection reagent 310: including 50KU / L urease, 1.0g / L trehalose, 20g / L chromogenic substance, 0.15g / L BSA, 0.1g / L sodium chloride. Add the prepared detection reagent 310 dropwise to the corresponding through hole 110 of the membrane material 320 with a dispenser. After 20 minutes, the reaction layer 300 was obtained.

[0059] Step 4: Paste a blood filter membrane on the reaction l...

Embodiment 2

[0062] In the preparation method of urea nitrogen detection test paper 1 in this embodiment, the detection reagent 310 includes 55KU / L urease, 1.2g / L trehalose, 22g / L chromogenic substance, 0.20g / L BSA, 0.12g / L Sodium chloride. Other steps are all the same as in Example 1.

Embodiment 3

[0064] In the preparation method of urea nitrogen detection test paper 1 in this embodiment, the detection reagent 310 includes 57.5KU / L urease, 1.3g / L trehalose, 25g / L chromogenic substance, 0.15g / L BSA, 0.13g / L of sodium chloride. Other steps are all the same as in Example 1.

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Abstract

The invention discloses a detecting reagent of urea nitrogen and detecting test paper of urea nitrogen. The detecting reagent contains 50-64 KU / L urease, 1.0-1.5 g / L trehalose and 20-30 g / L developing matter. According to the detecting reagent of urea nitrogen, multiple specific enzymes are specifically matched, and the aim of fast and accurately detecting the content of urea nitrogen can be achieved. The detecting test paper of urea nitrogen comprises a reaction base layer, a reaction layer, a flood filtering layer and a sample suction layer. The layers are overlapped to form a siphon system, the anti-interference capacity is higher, interference of endogenous substances is effectively eliminated, and it is further guaranteed that the content of urea nitrogen is fast and accurately detected.

Description

technical field [0001] The invention relates to the technical field of urea nitrogen detection, in particular to a urea nitrogen detection reagent and a urea nitrogen detection test paper. Background technique [0002] Currently, methods for detecting the presence or amount of a certain component in blood include liquid reagent testing and dry chemical test strip testing. Dry chemical test paper has the characteristics of convenient use, simple operation and quick results. [0003] According to the flow path of the liquid sample on the dry chemical test paper, the dry chemical test paper can be divided into various types, and the vertical vertical detection test paper is a kind of dry chemical test paper. The vertical vertical detection test paper includes stacking the carriers with the reaction reagents on top of each other, the liquid sample flows through each reaction reagent layer from top to bottom, and finally displays the detection results. For example, U.S. Patent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52
CPCG01N33/525
Inventor 彭勇李文韬李小慧
Owner 复星诊断科技(长沙)有限公司
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