The application belongs to the technical field of
urea nitrogen detection, and particularly relates to an integrated
microfluidic chip device and method for detecting
urea nitrogen; wherein a cover plate is bonded to a main plate, and the main plate is bonded to a base plate; the main plate is provided with a light path channel, a micro flow channel, a
reaction chamber, a
sample chamber, a control chamber and a groove; a photoelectric
detector is fixed in the groove, an LED
light source, a lens, a spectroscope and a reflecting mirror are fixed in the light path channel, the
sample chamber and the
reaction chamber are communicated through the micro flow channel, and the
sample chamber and the outside are communicated through the micro flow channel; the cover plate is etched with an
injection port communicated with the
reaction chamber and the control chamber; the application adopts the
microfluidic analysis chip idea, and through
microfabrication technology, functional elements such as the LED
light source, the lens, the spectroscope, the reflecting mirror, the reaction chamber, the sample chamber, the control chamber, the micro flow channel, the photoelectric
detector and the main control
chip are integrated on the
chip like integrated circuits, and the idea of transferring the functions of the whole laboratory to the chip is concentratedly embodied.