28 results about "Urea nitrogen" patented technology

The invention discloses a xanthine oxidase inhibitory peptide with a kidney improving function and application thereof, and belongs to the technical field of biological medicines.The xanthine oxidase inhibitory peptide is screened out by conducting enzymolysis, separation and purification on litopenaeus vannamei processing by-products and utilizing a molecular docking technology, the amino acid sequence of the xanthine oxidase inhibitory peptide is Pro-Leu-Gly-Pro-Pro-Pro, and the amino acid sequence of the xanthine oxidase inhibitory peptide is Pro-Leu-Gly-Pro-Pro-Pro. And the half inhibition concentration of the polypeptide is 2.356 + / -0.2448 mM. The xanthine oxidase inhibitory peptide is proved to have an improvement effect on hyperuricemia mice, the improvement effect is mainly characterized by reducing the content of serum uric acid, creatinine and urea nitrogen of the hyperuricemia mice, inhibiting the activity of xanthine oxidase (XOD) in serum and livers of the mice, inhibiting synthesis of uric acid and improving kidney injury of the mice, and Toxinpred prediction shows that the xanthine oxidase inhibitory peptide has the effect of inhibiting the activity of xanthine oxidase (XOD) in the serum and livers of the mice. The xanthine oxidase inhibitory peptide is free of toxic and side effects, so that the xanthine oxidase inhibitory peptide has a wide application prospect in preparation of food or drugs for improving renal functions.

The invention discloses a xanthine oxidase inhibitory peptide with uric acid reducing activity and application thereof, and belongs to the technical field of biological medicines.The xanthine oxidase inhibitory peptide is obtained by conducting enzymolysis, separation and purification on processing byproducts such as litopenaeus vannamei heads and shells with xanthine oxidase inhibitory activity. The xanthine oxidase inhibitory peptide is screened by using a molecular docking technology, and the amino acid sequence of the xanthine oxidase inhibitory peptide is Pro-Pro-Val-Pro-Pro-Pro. The xanthine oxidase inhibitory peptide has an XOD (X-Oxidase) half inhibitory concentration of 3.181 + / -0.1786 mM. The invention further proves that the xanthine oxidase inhibitory peptide has a certain improvement effect on hyperuricemia mice, and the xanthine oxidase inhibitory peptide mainly has the effects of reducing the content of serum uric acid, creatinine and urea nitrogen of the hyperuricemia mice, inhibiting the activity of xanthine oxidase (XOD) in serum and liver of the mice, inhibiting synthesis of uric acid and improving kidney injury of the mice, and the xanthine oxidase inhibitory peptide has a certain improvement effect on the hyperuricemia mice through Toxinpred prediction. The xanthine oxidase inhibitory peptide uric acid reducing peptide is free of toxic and side effects and has a wide market application prospect.

The invention discloses an intestinal strain capable of effectively regulating uric acid metabolism, a microbial agent of the intestinal strain and application of the intestinal strain in uric acid reducing products, and belongs to the technical field of microorganisms. According to the enterococcus pluvialis ZQ205, the enterococcus pluvialis ZQ205 which is free of toxic and side effects and capable of effectively relieving and treating the hyperuricemia is found for the first time, and the collection number of the enterococcus pluvialis ZQ205 in the China Center for Type Culture Collection is CCTCC NO: M 2025844; the strain can significantly reduce the levels of uric acid, creatinine and urea nitrogen in serum of mice, can effectively reduce the levels of inflammatory factors such as IL-6 and TNF-alpha in serum of hyperuricemia mice, and can improve kidney injury caused by hyperuricemia.

The invention discloses a fungus extract capable of effectively regulating uric acid metabolism and relieving inflammation as well as a preparation method and application of the fungus extract, and belongs to the technical field of deep processing of edible fungi. Schizophyllumcommuneh and poplar phellinus sporocarp are mixed and extracted, and the prepared fungus extract can effectively inhibit activity of xanthine oxidase in serum and liver of mice, reduce content of uric acid, creatinine, urea nitrogen and the like in serum and promote excretion of uric acid, creatinine, urea nitrogen and the like in urine; meanwhile, the content of inflammatory markers such as IL-6 and TNF-alpha in mouse serum can be effectively reduced, inflammatory infiltration of liver and kidney cells is relieved, and the problem that an existing edible mushroom extract is not ideal in treatment effect on hyperuricemia is solved.

The present invention relates to a uric acid-lowering probiotic complex and its application. The probiotic complex comprises Lactobacillus reuteri FPH2951 and Bifidobacterium breve FPHC4024; wherein Lactobacillus reuteri FPH2951 is classified as Lactobacillus reuteri and has a deposit number of GDMCC No. 62946; and Bifidobacterium breve FPHC4024 is classified as Bifidobacterium breve and has a deposit number of GDMCC No. 62945. The probiotic complex of the present invention can significantly reduce serum uric acid (UA), creatinine (Cr), and blood urea nitrogen (BUN) levels and increase urine excretion of creatinine (Cr) and blood urea nitrogen (BUN) through the synergistic effect of Lactobacillus reuteri FPH2951 and Bifidobacterium breve FPHC4024, and has broad application prospects.

The invention provides a combined lactobacillus salivarius YS-UR90 and a preparation method of the combined lactobacillus salivarius YS-UR90. The saliva combined lactobacillus provided by the invention can secrete protease and glycosidase, and the HCT and FLS gene expression levels of the saliva combined lactobacillus are high, so that the saliva combined lactobacillus can promote phenolic acid active ingredients to generate biotransformation during fermentation, and the proportion of chlorogenic acid and the like in fermentation liquor is increased. For example, marine fish oligopeptide and casein phosphopeptides are used as a composite nitrogen source, dandelion powder is used as a functional substrate, and a composite fermentation product obtained through fermentation of saliva and lactobacillus has the effect of promoting uric acid excretion and has a certain protection effect on kidney injury. The compound fermentation product has the effect of improving hyperuricemia mainly by inhibiting XOD activity, increasing serum urea nitrogen level, improving defect expression of uric acid synthesis gene HPRT1, inhibiting expression of uric acid reabsorption URAT1 and GLUT9 genes and improving expression of excretion genes OAT1 and ABCG2.

The invention provides an application of ACR11 protein in improving the content of plant protein and the utilization efficiency of urea, and provides an application of the ACR11 protein in improving the yield of the plant protein and / or improving the utilization efficiency of the plant to a urea nitrogen fertilizer, and the ACR11 protein can improve the yield of the plant protein and improve the utilization efficiency of the plant to the urea nitrogen fertilizer. The ACR11 protein disclosed by the invention can also improve the utilization efficiency of a plant on urea nitrogen fertilizer, and is specifically characterized in that a transgenic plant formed by transferring the coding gene of the duckweed ACR11 protein disclosed by the invention into a receptor plant is cultured under the condition of taking urea as a nitrogen source; compared with a receptor plant cultured under the same condition, the transgenic plant has higher biomass, higher protein content, higher protein yield and higher protein production efficiency under the same urea nitrogen fertilizer input. The invention provides a new way for improving the utilization efficiency of urea by crops and improving the protein yield of the crops in agriculture, and is beneficial to promoting the sustainable development of green agriculture.

The invention discloses a walnut-derived xanthine oxidase inhibitory peptide and application thereof, and belongs to the technical field of bioactive peptide biology. The amino acid sequence of the xanthine oxidase inhibitory peptide provided by the invention is as shown in SEQ ID No. 1. Through in-vitro activity determination, the IC50 value of the inhibitory peptide is 3.34 mg / mL, and it is proved that the inhibitory peptide has high inhibitory activity on xanthine oxidase. Besides, the inhibitory peptide can reduce the levels of creatinine, urea nitrogen, adenosine deaminase, glutamic oxalacetic transaminase and glutamic-pyruvic transaminase, regulate the expression of kidney uric acid resorption protein URAT1, uric acid excretion protein GLUT9 and ABCG2, and significantly reduce the generation of uric acid, can be used for inhibiting xanthine oxidase and preventing or improving hyperuricemia, is applied to preparation of drugs for improving hyperuricemia, and has broad application prospects. The medicine takes the active peptide as the only active ingredient or one of the active ingredients, and has a good application prospect.

The invention provides lactobacillus reuteri and an application thereof. The lactobacillus reuteri provided by the invention has a preservation number of CGMCC (China General Microbiological Culture Collection Center) NO. 32834 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. The lactobacillus reuteri provided by the invention can inhibit the activity of xanthine oxidase, promote the degradation of creatinine and guanosine, remarkably reduce the content of blood uric acid, reduce the content of creatinine and urea nitrogen, slow down tissue damage caused by hyperuricemia, improve the health level of damaged tissues caused by hyperuricemia, and improve the quality of the tissue damaged by hyperuricemia. Meanwhile, the probiotics have multiple beneficial effects in the aspects of eliminating inflammation, enhancing the immune function and the like, and the problem that existing uric acid reducing probiotics are insufficient in effectiveness is solved. The application can be used for developing microecological preparations, functional foods or nutritional supplements which are mainly used for reducing uric acid and have multiple effects (one bacterium has multiple purposes), and has a good industrialization prospect.

1. Name of the designed product: packing bag (containing phosphorus urea nitrogen). 2. Use of the designed product: used for packing bag of fertilizer. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo showing the design points: front view. 5. The designed product is a thin product, and other views are omitted.

The invention belongs to the technical field of fertilizers, and particularly relates to PBAT-based environment-friendly slow-release coated urea and a preparation method thereof. The PBAT-based environment-friendly slow-release coated urea provided by the invention comprises urea particles and a coating layer coating the urea particles, and the coating layer comprises the following raw materials: PBAT, an organic matter modifier and an inorganic matter modifier; the organic matter modifier comprises at least one of methyl cellulose or polyvinyl ether, and the inorganic matter modifier comprises at least one of bentonite or hydrotalcite. The coating material used in the PBAT-based environment-friendly slow-release coated urea has good biodegradability, degradation products have little harm to soil, the time required for the nitrogen nutrient release amount of the PBAT-based environment-friendly slow-release coated urea to reach 90% is 80-120 days, and the PBAT-based environment-friendly slow-release coated urea is suitable for growth of crops such as corn, soybeans and rice.

One embodiment of a blood urea nitrogen (BUN) sensor measures urea nitrogen in a biological sample by measuring a differential voltage signal based on first and second voltage signals, the first and second voltage signals each representing a detected amount of carbon dioxide. The first signal represents a detected amount of carbon dioxide resulting from an enzymatic reaction of a biological sample contacting a sensor surface having urease immobilized thereon. The second voltage signal represents a detected amount of background carbon dioxide in the biological sample. The differential voltage signal is indicative of the amount of urea nitrogen in the biological sample, which can be determined by an appropriate correlation. Other embodiments of a BUN sensor and a method of sensing a BUN are also provided, as are other aspects.

The invention provides a pharmaceutical composition for preventing, treating or relieving uric acid metabolic disorder and application, the pharmaceutical composition comprises a xanthine oxidase inhibitor and a URAT1 inhibitor, and the xanthine oxidase inhibitor is selected from teguxostat or febuxostat; the URAT1 inhibitor is selected from the group consisting of dotenod, Velnod, Lesinurad, Ruzinurad, SAP-001, ABP-671, SHR4640 and the like. The pharmaceutical composition provided by the invention can effectively reduce the concentration of uric acid in serum of a patient with hyperuricemia, so that the hyperuricemia is effectively prevented, treated or relieved. Compared with a single drug and the sum thereof, the combined drug composition can effectively inhibit serum uric acid, serum creatinine and urea nitrogen.

The application belongs to the technical field of urea nitrogen detection, and particularly relates to an integrated microfluidic chip device and method for detecting urea nitrogen; wherein a cover plate is bonded to a main plate, and the main plate is bonded to a base plate; the main plate is provided with a light path channel, a micro flow channel, a reaction chamber, a sample chamber, a control chamber and a groove; a photoelectric detector is fixed in the groove, an LED light source, a lens, a spectroscope and a reflecting mirror are fixed in the light path channel, the sample chamber and the reaction chamber are communicated through the micro flow channel, and the sample chamber and the outside are communicated through the micro flow channel; the cover plate is etched with an injection port communicated with the reaction chamber and the control chamber; the application adopts the microfluidic analysis chip idea, and through microfabrication technology, functional elements such as the LED light source, the lens, the spectroscope, the reflecting mirror, the reaction chamber, the sample chamber, the control chamber, the micro flow channel, the photoelectric detector and the main control chip are integrated on the chip like integrated circuits, and the idea of transferring the functions of the whole laboratory to the chip is concentratedly embodied.

The present invention discloses a composition for synergistically relieving physical fatigue and its application. The composition of the present invention can synergistically regulate anti-oxidation, calcium ion metabolism and energy metabolism, synergistically improve the body's ATP content and glycogen level, accelerate the clearance of lactic acid and urea nitrogen, prolong exercise time, and significantly relieve physical fatigue. Its synergistic effect is stronger than that of using red ginseng, black wolfberry and ganoderma lucidum alone. At the same time, the ingredients of the composition of the present invention are simple and all derived from natural plants; at the same time, after scientific demonstration and experimental verification, the mechanism of action of the composition is clear, and the fatigue-relieving interaction relationship between the various ingredients is clear, with the advantages of being green and safe. In addition, the preparation process of the composition of the present invention is simple and easy to operate, and does not require the investment of professional and technical personnel and large-scale instruments and equipment. The production cost is low, which is convenient for industrial development.

The invention discloses oral liquid with uric acid reducing and kidney protecting effects and a preparation method and application thereof, and belongs to the technical field of biology. The oral liquid with the effects of reducing uric acid and protecting kidney comprises the following raw materials: dendrobium officinale, American ginseng, taurine and a tea extract, and auxiliary materials comprise xylitol, potassium sorbate and purified water. Dendrobium officinale and American ginseng are decocted and purified and then are mixed with other raw materials. Experiments prove that the oral liquid not only can improve weight growth, reduce blood uric acid and blood urea nitrogen levels and blood xanthine oxidase activity, but also can improve kidney indexes of hyperuricemia mice, exert an anti-oxidation effect, inhibit inflammatory response and relieve pathological damage of renal tissues, thereby exerting the effects of reducing uric acid and protecting kidneys.

The present invention relates to a bacterial agent for alleviating hyperuricemia and its application. The bacterial strain in the bacterial agent for alleviating hyperuricemia is Akkermansia muciniphila. Akkermansia muciniphila Akk09 strain and Bifidobacterium animalis subsp. lactis Bifidobacterium animalum subsp. milk The two strains are composed of the BLa80 strain. They complement and enhance each other, synergizing their effects in alleviating hyperuricemia, specifically by lowering blood uric acid levels; inhibiting xanthine oxidase activity; reducing urea nitrogen and creatinine levels; alleviating kidney inflammation and oxidative stress caused by hyperuricemia; and regulating intestinal flora disturbances caused by hyperuricemia.

The invention discloses fermentation mucus lactobacillus with a uric acid reducing function and application of the fermentation mucus lactobacillus. The fermentation mucus lactobacillus is preserved in the China General Microbiological Culture Collection Center on August 4, 2025, and the preservation number is CGMCC No.35505. The invention further discloses a preparation method of the fermentation mucus lactobacillus. The fermented lactobacillus mucus has excellent creatinine degradation capability, can reduce serum uric acid, creatinine and urea nitrogen levels of an individual with hyperuricemia, and alleviates liver or kidney injury caused by the individual with hyperuricemia; in addition, the fermented lactobacillus mucus can also improve the composition of intestinal flora and inhibit the abnormal change of the flora related to metabolic disorder, thereby restoring the stable state of the microecology of the intestinal tract. Therefore, the fermented lactobacillus mucilaginosus can be used for preparing uric acid reducing functional foods, health foods or microecological preparations.

The application discloses use of allicin in promoting urea nitrogen conversion into nitrogen element in nucleotide or amino acid. The application finds through experiments that plant source compound allicin has the effect of promoting rumen urea nitrogen conversion into nitrogen element in nucleotide, sugar nucleotide or amino acid, thus, feeding ruminants with allicin directly or adding allicin into the basic daily ration of ruminants as a feed additive can promote urea nitrogen conversion into nitrogen element in nucleotide, sugar nucleotide or amino acid in the feed; the application further provides a method for promoting ruminants to convert urea nitrogen into nitrogen element in nucleotide, sugar nucleotide or amino acid for non-treatment purposes, which comprises: feeding ruminants with allicin directly or as a feed additive. The application has application prospects in providing high-quality protein sources for livestock and poultry, reducing feed costs and improving production performance of livestock and poultry and the like.

The utility model belongs to the technical field of detection card, concretely relates to a urea nitrogen detection card, including card shell, slide and membrane material assembly spare, membrane material assembly spare is embedded in the inside of card shell, one side of card shell is provided with sample adding port no.

The invention relates to the technical field of medical instruments, and provides a dialysis urea clearance rate online monitoring method, a computer system and a monitoring system.The monitoring method comprises the steps that S1, a detection light source is adopted to irradiate dialysis waste liquid, and the incident light intensity I0 is recorded; s2, collecting the initial transmission light intensity I1 penetrating through the dialysis waste liquid and the transmission light intensity It at the current t moment, and calculating the ratio Rt of the urea nitrogen concentration after dialysis to the urea nitrogen concentration before dialysis on the basis of I1 and It; and S3, calculating a urea clearance index Kt / Vt value at the current t moment based on the Rt. The method has the advantages that the final urea clearance index Kt / Vt value is obtained according to the data of the illumination intensity of the dialysis waste liquid, real-time monitoring of urea data is achieved, the accuracy of the final urea clearance rate calculation result is improved, medical staff can conveniently and timely obtain the urea clearance condition of a dialyzer on blood, and the medical staff can conveniently and rapidly know the urea clearance condition of the dialyzer on the blood. In addition, invasive operation in a traditional blood sampling measurement mode is avoided, discomfort brought to a patient is avoided, and the infection risk is reduced.

One embodiment of a blood urea nitrogen (BUN) sensor measures urea nitrogen in a biological sample by measuring a differential voltage signal based on first and second voltage signals, each representing the amount of carbon dioxide detected. The first signal represents the amount of detected carbon dioxide resulting from an enzymatic reaction in the biological sample where a urease enzyme comes into contact with the immobilized sensor surface. The second voltage signal represents the amount of background carbon dioxide detected in the biological sample. The differential voltage signal indicates the amount of urea nitrogen in the biological sample, which can be determined by appropriate correlation. Other embodiments of the BUN sensor and methods for detecting BUN are also provided, as in other embodiments.

The present invention relates to the field of dialysis technology, and specifically to an AI-based dialysis scheme optimization system, the system comprising: based on continuous dialysis cycle data, analyzing changes in urea nitrogen concentration and dialysate discharge volume, obtaining dynamic characteristics of cycle nitrogen, and then optimizing sodium concentration settings, combining abnormal linkage of blood flow rate, heart rate and systolic pressure, outputting physiological linkage warning signals, adjusting ultrafiltration volume and dialysis duration, and generating equipment control instructions. The present invention, by realizing multi-parameter trend induction and real-time linkage control, can analyze the complex coupling relationship between multi-dimensional physiological signals in a continuous dialysis cycle, automatically identify key metabolic states and dynamic changes in parameters, drive adaptive responses of core parameters such as sodium concentration and ultrafiltration operation, and output dynamic control instructions for hemodynamics and volume abnormalities based on the risk signal generation mechanism, so that the dialysis process has the capabilities of intelligent discrimination, automatic sorting and rapid matching, and forms an optimization strategy for individual conditions.

The present application relates to the technical field of reducing uric acid, and discloses a composition capable of reducing uric acid, which comprises 20-30 parts of chicory extract, 15-20 parts of coix seed powder, 10-20 parts of sunflower disc extract, 9-15 parts of galangal extract, 10-15 parts of polygonatum extract and 7-15 parts of xanthine oxidase inhibiting peptide; the xanthine oxidase inhibiting peptide is extracted by enzymolysis of bonito processing byproducts. The composition has a high inhibiting effect on XOD activity, can reduce the levels of creatinine and urea nitrogen, realizes uric acid reduction, and has the effects of inhibiting renal tubular expansion and reducing renal interstitial inflammatory cell infiltration, and has the effect of protecting kidney. Meanwhile, the bonito processing byproducts are utilized by preparing XOD inhibiting active polypeptide, a new utilization way of bonito processing byproducts is developed, and the utilization value of bonito processing byproducts is improved.

The invention belongs to the technical field of functional microorganisms and application thereof, and relates to compound lactic acid bacteria and application thereof in preparation of products for relieving hyperuricemia. The compound lactobacillus is prepared from lactobacillus plantarum PL4-9, pediococcus acidilactici FB1-3 and lactobacillus plantarum sc3-2, and the preservation numbers of the lactobacillus plantarum PL4-9, the pediococcus acidilactici FB1-3 and the lactobacillus plantarum sc3-2 are CGMCC (China General Microbiological Culture Collection Center) NO. 34075, CGMCC NO. 34076 and The compound lactic acid bacteria formed by mixing the raw materials according to the volume ratio of 1: 1: 1 can obviously reduce the serum uric acid and urea nitrogen level of a mouse with hyperuricemia, and also has an obvious relieving effect on abnormal increase of serum xanthine oxidase activity; the generation of uric acid can be reduced by inhibiting the activity of liver xanthine oxidase, and meanwhile, the liver and kidney injury induced by hyperuricemia can be remarkably relieved.

The invention relates to a lactobacillus plantarum LP-SG7, a postbiotics, a composition and application. The lactobacillus plantarum LP-SG7 provided by the invention is high in biological activity and can tolerate gastric acid and cholate. The lactobacillus plantarum LP-SG7 or the composition containing the viable bacteria of the lactobacillus plantarum LP-SG7 and the metagenic powder described in the invention can significantly reduce the content of blood uric acid, urea nitrogen and creatinine in a model mouse with oteracil potassium induced hyperuricemia, and reduce the content of xanthine oxygen in liver tissues.

A walnut-derived xanthine oxidase inhibitory peptide and its application belong to the field of bioactive peptide biotechnology. The amino acid sequence of the xanthine oxidase inhibitory peptide provided by this invention is shown in SEQ ID No. 1. In vitro activity assays showed that the IC50 of this inhibitory peptide... 50 The concentration was 3.34 mg / mL, demonstrating its high inhibitory activity against xanthine oxidase. Furthermore, this inhibitory peptide can reduce the levels of creatinine, blood urea nitrogen, adenosine deaminase, aspartate aminotransferase, and alanine aminotransferase, regulate the expression of renal uric acid reabsorption protein URAT1, uric acid excretion protein GLUT9, and ABCG2, and significantly reduce uric acid production. It can be used to inhibit xanthine oxidase, prevent or improve hyperuricemia, and is applicable to the preparation of drugs for improving hyperuricemia. These drugs, with the active peptide as the sole active ingredient or one of its active ingredients, have promising application prospects.

The invention provides an application of glutamate dehydrogenase GDH in improving the content of plant protein and the utilization efficiency of urea, and provides an application of the glutamate dehydrogenase GDH in improving the yield of plant protein and / or improving the utilization efficiency of urea nitrogen fertilizer by plants, and the glutamate dehydrogenase GDH can improve the yield of plant protein and improve the utilization efficiency of urea nitrogen fertilizer by plants. The glutamate dehydrogenase GDH provided by the invention can also improve the utilization efficiency of urea nitrogen fertilizer by plants, and is specifically characterized in that a transgenic plant formed by transferring the encoding gene GDH1 of the Lemna minor glutamate dehydrogenase into a receptor plant is cultured under the condition of taking urea as a nitrogen source; compared with the receptor plant, the transgenic plant has higher protein content and urea nitrogen fertilizer utilization efficiency. The invention provides a new way for increasing the protein content of crops and increasing the utilization efficiency of urea by the crops in agriculture, and has important significance for sustainable development of green agriculture.