Lactobacillus plantarum UA-416 strain and application thereof

A technology of UA-416 and Lactobacillus plantarum, applied in the field of Lactobacillus plantarum UA-416 strain, can solve the problem of low uric acid reduction of lactic acid bacteria, and achieve the effect of huge market space, broad application prospect and wide audience.

Active Publication Date: 2018-05-18
JILIN MINGZHIYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports about lactic acid bacteria reducing uric acid and anti-gout

Method used

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  • Lactobacillus plantarum UA-416 strain and application thereof
  • Lactobacillus plantarum UA-416 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1 The preparation of plant lactobacillus (Lactobacillus plantarum) UA-416 strain of the present invention

[0051] 1. Isolation of Lactobacillus plantarum strains

[0052] 1. Sample material: traditional fermented food chili sauce.

[0053] Take 5g of solid hot sauce, add 45mL of normal saline (10-fold dilution) and mix well, take 0.5mL of diluent, and dilute to 10 times with normal saline again -3 , take 100μL liquid (10 -1 、10 -2 、10 -3 ) in solid BCP medium for cultivation (cultivation temperature 37°C, cultivation time 48-72h). Pick a single colony that produces a yellow circle and inoculate it on a solid MRS medium plate to continue culturing (cultivation temperature 37°C, culture time 48h), and repeatedly streak culture and purify on a solid MRS medium plate (cultivation temperature 37°C, culture time 48h) , to obtain multiple strains of pure culture strains.

[0054] 2. Inoculate the multiple strains of purely cultured strains obtained above into...

Embodiment 2

[0088] Example 2 Effect of plant lactobacillus (Lactobacillus plantarum) UA-416 strain of the present invention on hyperuricemia in mice induced by oxonic acid potassium salt

[0089] 1. Establishment and grouping of animal models

[0090] 60 male Kunming mice, weighing 22-25g, were randomly divided into 3 groups, 20 in each group, and the grouping treatment was as follows: the model group and the UA-416 group were treated with canned stomach yeast extract (15g / kg) twice a day for 7 consecutive days, ethylamine Butanol (250mg / kg) once a day, the concentration of UA-416 group was 1.0×10 9 CFU / mL Lactobacillus plantarum UA-416 bacterial solution (12ml / kg body weight). 2 hours after the last administration, a one-time intraperitoneal injection of oxonate potassium (300 mg / kg). The blank control group was given the same dose of gastric or intraperitoneal injection of sterilized normal saline. After the experiment, the mice in each group were removed from the eyeballs to collect...

Embodiment 3

[0096] Example 3 Preparation of fermented uric acid-lowering medicine and food homologous composition

[0097] 1. Preparation of Lactobacillus plantarum UA-416 starter

[0098] Plantarum UA-416 was expanded to 20L, centrifuged at 6000r / min for 20min, resuspended in normal saline, centrifuged again at 6000r / min for 20min, resuspended in normal saline and washed, centrifuged again at 6000r / min for 20min, collected the sludge, dissolved in In 500mL of freeze-drying protectant, freeze-dry to get Lactobacillus plantarum UA-416 fermentation agent, the number of bacteria is about 1.0×10 9 CFU / g.

[0099] The formula of the lyoprotectant used: 90g of fructooligosaccharide and 10g of trehalose were dissolved in tertiary water, and the volume was adjusted to 500mL.

[0100] 2. Preparation of fermented uric acid-lowering medicine and food homologous composition

[0101] Mix 30 to 50 parts of corn silk, 20 to 40 parts of celery seed, 10 to 30 parts of mulberry, and 10 to 20 parts of ac...

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Abstract

The invention discloses a lactobacillus plantarum UA-416 strain and application thereof, and belongs to the field of functional food microbes. Blanks of lactobacillus plantarum in the effects of lowering uric acid and resisting gout are filled up. According to the lactobacillus plantarum (Lactobacillus plantarum) UA-416 strain provided by the invention, the bacterial strain is already collected inthe China Center for Type Culture Collection (CCTCC) on July 25, 2017; the collection number is CCTCC NO: M2017419. The lactobacillus plantarum UA-416 strain provided by the invention can be used foreffectively lowering the level of blood uric acid, is used for improving the levels of urea nitrogen and creatinine and inhibiting the activity of xanthine oxidase in serum, and has prevention and treatment effects on uricemia or the gout.

Description

technical field [0001] The invention belongs to the technical field of functional food microorganisms, and in particular relates to a strain of Lactobacillus plantarum UA-416 and an application thereof. Background technique [0002] Gout is a group of heterogeneous diseases that cause tissue damage caused by long-term purine metabolism disorders or elevated blood uric acid. The biochemical marker of gout is hyperuricemia, which refers to the supersaturated state of urate in the extracellular fluid. It is generally believed that hyperuricemia occurs when the blood uric acid is 416 μmol / L, and about 5-12% of patients with hyperuricemia will develop into hyperuricemia. gout. The acute attack of gout is an acute inflammatory reaction caused by the deposition of sodium urate in the form of crystals in joints and tissues around the joints. Gout can not only invade bones and joints, but also easily involve the kidneys and cardiovascular system. Hyperuricemia and primary gout are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P19/06C12R1/25
CPCA23L33/135C12N1/20A23V2002/00A61K2035/115C12R2001/25C12N1/205A23V2400/169A23V2200/30
Inventor 崔伟东梁铁
Owner JILIN MINGZHIYUAN BIOTECH
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