Preparation of phellinus igniarius sporocarp phenolic active substance and application of phellinus igniarius sporocarp phenolic active substance in regulation of intestinal flora and uric acid metabolism

An active substance, the technology of Sanghuangzi, which is applied in the direction of drug combination, food science, and resistance to vector-borne diseases, etc., can solve the problems of mixed functional components, low extraction rate and insufficient extraction of polyphenol active substances, and achieve enhanced The excretion of uric acid, the preparation method is simple and efficient, and the effect of reducing serum uric acid

Active Publication Date: 2022-08-02
FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the current problems of low extraction rate of polyphenol active substances, insufficient extraction, and mixed functional components in the fruiting body of Phellinus phellifolia, the first object of the present invention is to provide a high-purity mulberry polyphenol content of more than 60%. Preparation method of yellow fruiting body phenolic active substance

Method used

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  • Preparation of phellinus igniarius sporocarp phenolic active substance and application of phellinus igniarius sporocarp phenolic active substance in regulation of intestinal flora and uric acid metabolism
  • Preparation of phellinus igniarius sporocarp phenolic active substance and application of phellinus igniarius sporocarp phenolic active substance in regulation of intestinal flora and uric acid metabolism
  • Preparation of phellinus igniarius sporocarp phenolic active substance and application of phellinus igniarius sporocarp phenolic active substance in regulation of intestinal flora and uric acid metabolism

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Get 100g of poplar Phellinus (Sanghuangporus vaninii) fruit body 100g of cultivation age 3 years, pulverize after oven dry and cross 40 mesh sieves, the fruit body powder that obtains is added the water of 5 times of amount to soak overnight, add acetone-ethanol mixture, make acetone: The volume ratio of ethanol:water is 10:80:10. After stirring evenly, the multi-frequency ultrasonic device is used for extraction. The multi-frequency ultrasonic device is set to: ultrasonic power 500W, extraction temperature 30 ℃; ultrasonic frequency 40KHz and 80KHz are used alternately, 5min each time, a total of 20min. 5000r / min centrifugation, then add acetone-ethanol-water mixture (V 丙酮 : V 乙醇 : V 水 =10:80:10), after stirring evenly, the extraction was repeated once with a multi-frequency ultrasonic device, the supernatants of the two times were combined, and 6.98 g of acetone ethanol crude extract was obtained after concentration by rotary evaporation (extraction rate was 6.98%)....

Embodiment 2

[0035] Embodiment 2: Effect experiment of phenolic active substance of Phellinus linteus fruiting body

[0036] 1. Experimental steps

[0037] (1) Using gallic acid as the standard, the total phenolic content in each group of samples was determined by the Folin-phenol method; using rutin as the standard, the flavonoid content in each group of samples was determined by the sodium nitrite-aluminum nitrate method.

[0038] (2) Determination of xanthine oxidase inhibitory activity: take 0.6 mL of phosphate buffer solution (0.1 mol / L, pH=8.5), 0.2 mL of xanthine solution (2 mmol / L) and different solutions (3.0 mg / mL, DMSO Preparation) 0.1mL was vortexed in a 1.5mL centrifuge tube; then 0.2mL xanthine oxidase solution (0.1U / mL) was added and reacted in a water bath at 25°C for 30min; after completion, 0.2mL HCl solution (1.0mol / mL) was added L) Terminate the reaction. Using allopurinol as a positive control, the absorbance was measured at a wavelength of 290 nm. The formula for c...

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Abstract

The invention provides preparation and application of phellinus igniarius sporocarp phenolic active substances, and the method comprises the following steps: by taking phellinus igniarius sporocarp powder as a raw material, firstly soaking the phellinus igniarius sporocarp powder in water, and then adding an acetone-ethanol mixed solution for multi-frequency ultrasonic extraction; after a crude product is obtained, carrying out fractional extraction by adopting an ethyl acetate and n-butyl alcohol mixed solution; after an extract is obtained, macromolecular substances such as polysaccharide and protein in a sample are removed by utilizing a membrane separation technology; and performing rotary evaporation and freeze-drying to obtain the phellinus igniarius phenol active substance. The phellinus igniarius sporocarp phenol active substance extracted by the method is relatively high in purity and relatively strong in activity, not only can effectively improve the diversity of intestinal flora and promote the growth of intestinal beneficial flora, but also can increase the excretion of uric acid, creatinine and urea nitrogen in urine and effectively reduce the content of uric acid, creatinine and urea nitrogen in serum; the product can be used as a natural auxiliary food for maintaining uric acid metabolism homeostasis and regulating intestinal flora without side effects.

Description

technical field [0001] The invention belongs to the technical field of deep processing of edible fungi, in particular to the preparation of an active substance of Phellinus linteus fruiting body and its application in the regulation of uric acid metabolism. Background technique [0002] Phellinus linteus, also known as mulberry ear, mulberry and sun eye, belongs to Basidiomycota, Agaricomycetes, Hymenochaetales, Hymenochaetaceae, Phellinus genus. Sanghuangporus), is a kind of precious medicinal fungi. Modern research has found that Phellinus linteus contains a variety of active substances such as polysaccharides, flavonoids, triterpenes, polyphenols, pyrones and furans, so it has antioxidant, immune regulation, hypoglycemic, anti-tumor and anti-cancer properties. kind of effect. In recent years, with the continuous deepening of the research on the pharmacological effects of Phellinus linteus and the popularity of its natural anti-cancer product properties, Phellinus linteu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/07A23L33/105A61P1/00A61P19/06
CPCA61K36/07A61P1/00A61P19/06A23L33/105A61K2236/333A61K2236/39A61K2236/53A61K2236/51A23V2002/00A23V2200/32A23V2200/30A23V2250/208Y02A50/30
Inventor 殷朝敏高虹范秀芝史德芳姚芬乔鑫李雨虹李江涛程雅清李晨刘梦凡卢琪薛淑静杨德
Owner FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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