174 results about "Phellinus linteus" patented technology

The invention relates to an artificial cultivation method for phellinus linteus. Artificial domestication cultivation research of the phellinus linteus newly starts in China, a few exploration tests are conducted in parts of areas, and the problems such as sporocarp is low in productivity and poor in quality (unshaped) exist mainly due to the fact that cultivation techniques are immature. The artificial cultivation method for the phellinus linteus comprises the steps that phellinus linteus strains are activated, 0.3 square centimeter stain blocks are taken on the aseptic condition to be inoculated to phellinus linteus cultivation culture media in cultivation bags, the stain blocks are cultivated at constant temperature being 28 DEG C without light until mycelia grow all over the cultivation bags, and cultispecies are obtained; when the mycelia in the cultivation bags are yellow or have protuberant nodular primordia, the cultivation bags are moved out of yellow areas, and semi-bag-off soil-cast yellow-out cultivation is conducted. According to the artificial cultivation method for the phellinus linteus, mulberry fields are used for phellinus linteus cultivation, and growing conditions of generation, differentiation and development of wild phellinus linteus sporocarps are imitated in nature. The phellinus linteus is high in productivity, good in quality (similar to wild phellinus linteus in shape), short in growth cycle, simple in cultivation mode, and capable of being cultivated in large scale.

The invention relates to a preparation method of a biological composite polysaccharide efficient nutrient solution. The invention comprises the steps of selecting a strain, preparing a nutrient, culturing a liquid spawn, generating a mycelium in a fermentor, filtrating the fermentation broth and generating the nutrient solution which is rich in microbial polysaccharide after concentration. The key of the invention lies in that the composite nutrient solution is made by mixing the mycelium culture medium extracted from the respective fermentor after deep fermentation of the following microbial strains after the above steps according to the following weight proportion: mythic fungus of 5 to 20 percent, aweto of 5 to 20 percent, poria of 5 to 15 percent, grey polypore of 5 to 15 percent, boletus of 5 to 15 percent, phellinus linteus of 5 to 10 percent, manyzoned polypore of 5 to 20 percent, mushroom of 6 to 20 percent, bailing of 5 to 10 percent mushroom and agaricus blazei strain of 5 to 20 percent. The nutrient solution is flask separated, sealed and sterilized under bacteria free environment to become the final product.

The invention aims at providing a mixed fungus ultrafine powder which reserves active ingredients maximumly, is easy to be absorbed and has multiple health care functions, and a preparation method thereof. The preparation method comprises the following steps: A. putting selected good Sparassia crispa fruit body, Trametes robiniophila Murr fruit body and Phellinus linteus fruit body into a vacuum freezing drying oven for drying, and slicing for standby; and putting liquid fermented Antrodia camphorate mycelium into the vacuum freezing drying oven, and drying for standby; B. respectively processing and grinding the four types of dry products obtained in the step A by a motor-driven grinder with 30-100 meshes of sieve mesh; C. processing and grinding coarse powders and Ganoderma spore obtained in step B by an ultrafine grinder with the 1000-2500 meshes of sieve mesh; and D. fully mixing the ultrafine powders obtained in step C by the following weight percent: 60-80% of Sparassia crispa fruit body ultrafine powders, 5-10% of Trametes robiniophila Murr fruit body ultrafine powders, 5-10% of Ganoderma lucidum karst spores, 5-10% of Phellinus linteus fruit body ultrafine powders, and 5-10% of Antrodia camphorate liquid fermentation mycelium ultrafine powders.

The invention discloses a Phellinus linteus polysaccharide separation and purification method which is characterized by mainly comprising the following steps: preparing a Phellinus linteus polysaccharide component by an ammonium oxalate extraction-ethanol precipitation process, carrying out anion exchange chromatography and gel filtration chromatography to separate and purify the polysaccharide, and carrying out concentration, dialysis, vacuum freeze drying and the like on the product to obtain the Phellinus linteus polysaccharide pure product. The method does not influence the chemical structure of the natural polysaccharide, and can keep the original bioactivity of the natural polysaccharide. The method has the advantages of low requirements for equipment and environment, simple process and low cost, is flexible and practical, and is beneficial to popularization, development and application.

The invention relates to a phellinus igniarius bacterial strain liquid fermenting culture medium and a method for fermenting and producing phellinus linteus polysaccharides. The culture medium has a prescription shown as follows: 20 to 30g/L of glucose, 2 to 6g/L of yeast powder, 1 to 3g/L of KH2PO4, 0.3 to 0.7g/L of MgSO4.7H2O, 110mg/L of VB, and 1L of water; a pH is under a natural condition; a fermenting condition is that a temperature is at 28 to 32 DEG C, a rocking speed is at 140 to 180rpm, inoculation amount is 20 to 30%, cultivation is carried out for 9 to 11 days; the method comprises the following steps of: centrifuging, collecting fermenting liquid, washing via distilled water, precipitating to obtain a mycelium, then extracting polysaccharide inside or outside cells. With the adoption of the phellinus igniarius bacterial strain liquid fermenting culture medium and the method for fermenting and producing the phellinus linteus polysaccharides provided by the invention, yield of the mycelium of phellinus igniarius and content of the polysaccharide inside or outside the cells can be obviously increased; moreover, yields of the phellinus linteus polysaccharides is 6.34% higher than that of polysaccharide extracted by a common hot water extracting method. An animal test proves that extracted phellinus linteus polysaccharides have obvious biological activity such as tumour resistance, oxidization resistance, and improvement of immunity.

The invention provides a synthetic medium for culturing a phellinus igniarius liquid. The synthetic medium takes glucose as a carbon source and 20 kinds of amino acid as a nitrogen source, and consists of inorganic salt and vitamin. The ratio of carbon to nitrogen is designed as 50 to 1; and the specific concentration g/l is as follows: the glucose: 30 to 80 grams; glutamic acid: 0.1 to 1.5 grams; glutamine: 0.1 to 1.5 grams; aspartic acid: 0.1 to 1.5 grams; asparagines: 0.1 to 1.5 grams; glutamic acid: 0.01 to 0.1 gram; tryptophan: 0.01 to 0.1 gram; valine: 0.01 to 0.1 gram; alanine: 0.01 to 0.1 gram; etc. During the whole culture process of the synthetic medium, phellinus igniarius mycelium is quick in growth; the yield of the phellinus igniarius mycelium can reach 20 grams per liter to the highest degree; simultaneously no agricultural and sideline product is introduced as the carbon source and the nitrogen source; substances such as proteins in the medium, medium compositions and so on are not carried; phellinus igniarius exopolysaccharide produced through fermentation has high purity and stable quality; and the yield of phellinus igniarius polysaccharide can reach 0.198 gram per liter to the highest degree. The synthetic medium has the advantages of clear compositions, stable quality and so on, and can be used for mass culture of the phellinus igniarius liquid and research of the regulation and control rule of polysaccharide anabolism.

The invention discloses lucid ganoderma tea, lucid ganoderma teabag and a processing technology thereof. The lucid ganoderma tea comprises the following four components in parts by weight: 50-60 parts of inonotus obliquus powder, 10-20 parts of phellinus igniarius powder, 10-20 parts of phellinus linteus powder and 5-30 parts of lucid ganoderma extract. Thus, the lucid ganoderma tea disclosed by the embodiment of the invention has perfect mouthfeel, can give full play to the medicinal effect and healthcare effect of lucid ganoderma, improves the body immunity, and realizes an effect of adjusting and protecting the central nervous system, digestive system, endocrine system and respiratory system and the organs such as liver, prostate and the like.

The invention discloses a method for extracting high-activity phellinus linteus polysaccharide at low pressure and low temperature. The method comprises the following steps: mixing male agaric mycelium obtained by a liquid fermentation method with deionized water evenly at the feed-to-liquid ratio of 1g to 40-60mL; extracting at -0.04MPa to -0.07MPa and 50-60 DEG C for 1-3 hours; and centrifuging and separating an extract liquid, and carrying out alcohol precipitation to obtain active phellinus linteus polysaccharide. The extraction method disclosed by the invention is simple, low in extraction cost, and easy to operate. Compared with a hot water extraction method and an ultrasonic assist extraction method, the yield of the phellinus linteus polysaccharide is increased; the active structure of the phellinus linteus polysaccharide can be well kept; the high-activity phellinus linteus polysaccharide is obtained.

The invention provides a phellinus linteus strain and a breeding method of the phellinus linteus strain. The breeding method comprises the steps of preparation and mutagenesis of protoplast, screening of a mutagenesis strain, and verification of the mutagenesis strain. The shake flask biomass of the phellinus linteus strain obtained through the breeding method reaches 17.92 g/L and is higher than that of an original strain by 9.51%. The yield of flavone reaches 0.47 g/L and is higher than that of the original strain by 52.16%, and the yield of polysaccharide reaches 3.75 g/L and is higher than that of the original strain by 67.95%. The biological yield and the yield of metabolic active matter are also remarkably higher than those of the parent strain SH1, and the free radical scavenging capability is remarkably improved.

The invention discloses a preparation method of a zinc-enriched liquid fermentation product of phellinus linteus. The preparation method comprises the following steps: inoculating a phellinus linteus strain onto a liquid seed culture medium added with zinc-enriched yeast and a diaphragma juglandis extract, rapidly culturing with the help of a low-frequency ultrasound with a certain frequency and intensity, inoculating the phellinus linteus liquid strain onto a liquid fermentation culture medium added with zinc-enriched yeast and a plukenetia volubilis leave enzymolysis product and culturing in an ultra-low-frequency magnetic field with a certain frequency and intensity to obtain the zinc-enriched liquid fermentation product of phellinus linteus. According to the preparation method, by the physical-assisted synergistic fermentation of seed liquid and fermentation two-phase low-frequency ultrasound and ultra-low-frequency magnetic fields and the synergistic effect of exogenous additives such as the diaphragma juglandis extract and the plukenetia volubilis leave enzymolysis product, the contents and activities of organic zinc and zinc polysaccharide in final fermentation product of phellinus linteus are significantly increased.

The present invention relates to a crude exopolysaccharides (EPS) produced from Phellinus baumii mycelium comprising mannose and/or arabinose and a preparation method thereof.

The invention relates to a preparation method of a composite solid-state probiotic agent including phellinus linteus. The method comprises the following steps of inoculating phellinus linteus seed fluid to solid-state fermentation culture mediums, performing fermentation, and degrading cellulose and hemicellulose type substances for producing monosaccharide; and then sequentially adding after fermentation bacterial fluid for a plurality of times, performing fermentation to obtain fermentation products, and then drying the fermentation products so as to obtain the composite solid-state probiotic agent. According to the preparation method disclosed by the invention, stagewise solid-state fermentation is performed according to requirement differences of different probiotics for nutrient substances, so that the culture mediums can be efficiently decomposed; phellinus linteus grows through solid-state substrates to produce active polysaccharide, and besides, the cellulose and hemicellulose type substances are degraded to generate the monosaccharide; and the solid-state probiotic agent has special paste fragrance, contains fungus polysaccharide, is high in effective viable count, is high in the content of organic nitrogen, and has the efficacy of adjusting the flora in the intestinal tracts of animals, enhancing immunity, increasing the utilization rate of the solid-state substrates, reducing the fetor of excrement of livestock and improving environment.

The invention discloses a preparation method of phellinus linteus polysaccharides. The preparation method comprises the following steps: (1) removing impurities from wild phellinus linteus and crushing the wild phellinus linteus; (2) freezing phellinus linteus in the step (1) to obtain freeze-dried powder; (3) breaking the wall of the freeze-dried powder obtained in step (2) in a low temperature environment to obtain phellinus linteus powder; (4) adding water to the phellinus linteus powder treated in the step (3) for leaching, taking a filtrate, and performing concentration under reduced pressure to obtain a water extract; (5) adding a mixture of ethanol, n-butanol and methanol to the water extract of the step (4) to obtain a precipitate; (6) washing the precipitate with an organic solvent and drying the precipitate to obtain the phellinus linteus polysaccharides. The preparation method of the phellinus linteus polysaccharides has the advantages that on the premise of maintaining activity of the phellinus linteus polysaccharides, the content and purity of the phellinus linteus polysaccharides are increased, and the medicinal value of the phellinus linteus polysaccharides is further improved.

The invention discloses mushroom concentrated liquid and drink containing beta-glucan, which is liquid type health-care drink prepared by mixing 5-50 percent of concentrated extract liquid of nine mushrooms of birch mushroom, phellinus linteus, ganoderma lucidum, grifola frondosa, hericium erinaceum, shiitake mushroom, sparasis crispa(Wulf)Fr., pine mushroom and aweto, 1.6-3 percent of sugar or sugar alcohol, 2.0-3.5 percent of honey, 0.5-0.8 percent of syrup and 0.01-0.03 percent of acid with water till 500ml. The drink is easy to be internally digested and absorbed by a human body, can improve the immunity resisting ability of the human body, and has the advantages of rich nutrition, enhancement and improvement of human immunity, virus resistance, fungus resistance, reduction of blood sugar, stimulation of hematopoiesis, reduction of cholesterin, reduction of blood lipid, aging resistance and the like, and can be used as a novel health-care type drink. The invention further discloses a method for preparing the mushroom concentrated liquid and drink containing delta-dextran.

A composition for promoting the proliferation of hematopoietic stem cells, which contains mushroom extracts, and more particularly to a composition and functional food for promoting the proliferation of hematopoietic stem cells, which contain, as active ingredients, an Inonotus Obliquus extract, a Ganoderma Luciderm extract, a Phellinus Linteus extract, and plant extracts promoting the immune activity-enhancing effects of these mushroom extracts. The composition and functional food assists in enhancing immune activity by significantly promoting the proliferation of hematopoietic stem cells, and shows positive effects on the increase in anticancer activity, anti-inflammatory activity and cell activity, the regeneration of damaged tissue and the prevention of cardiovascular diseases, through the proliferation of hematopoietic stem cells and the enhancement of immune activity.

The invention relates to a cultivation technology of fungi and in particular relates to a Korean phellinus linteus plastic bag cultivation method. The method comprises the following steps: adding a culture medium into a stirrer according to a formula ratio, and adding water for stirring; bagging by using polypropylene edible mushroom bags; sterilizing by using constant-pressure steam; reducing the temperature to 45-60 DEG C, and transferring into a sterile room for stacking, cooling to the temperature of 25-30 DEG C, and inoculating under sterile conditions; transferring into a culture room after inoculating, wherein the edible mushroom bags are full of mycelia within 30-40 days; transferring the mushroom bags full of mycelia into a fruiting room to enter a fruiting body cultivation stage, arranging the mushroom bags, and opening the bag cover until yellow mushrooms come out; picking the fruiting bodies within 120-180 days after maturation, wherein the picking manner is that the mushroom bag is held by one hand and the fruiting bodies are picked by the other hand; and drying and packaging. According to the method disclosed by the invention, the fruiting bodies of the Korean phellinus linteus and pharmacological active ingredients of the mycelium are maintained to the greatest degree; and by experiencing the method, the content of cultivated fruiting bodies and various components of the mycelium is equivalent to content of various components of wild phellinus linteus.

The invention belongs to the technical field of phellinus linteus cultivation, and provides a method for performing large-scale and stable cultivation on phellinus linteus. The method comprises the following steps: compounding zeolite powder and diatomite, performing high-temperature sterilization, rapidly cooling to a certain temperature by utilizing liquid nitrogen, and inoculating the phellinus linteus into a complexing body of the zeolite powder and the diatomite in a sterile room; prefabricating a culture medium by using water, mulberry sawdust, a carbon source, a nitrogen source, a phosphorus fertilizer, a potassium fertilizer, magnesium sulfate and agar powder, and performing sterilization by using high-pressure steam; mixing the complexing body inoculated with the phellinus linteus and a nutritional medium according to a proportion, putting the mixture into a grid plate, packaging by using plastic, putting into a culture room, controlling the temperature and the relative humidity of the culture room, maintaining a dark state, and cultivating for a certain time to obtain uniform mycelium. When the method is used for cultivating the phellinus linteus, the survival rate is high, the quality is stable and large-scale cultivation can be realized.

The invention relates to water-holding and moisturizing essence and a preparation method thereof. The water-holding and moisturizing essence is prepared from, by weight, a component A, a component B and a component C, wherein the component A comprises deionized water, dipropylene glycol, sodium hyaluronate (HA), NANO sodium hyaluronate, a PEG/PPG-17/6 copolymer, an amino acid moisturizing agent (trimethylglycine trimethylglycocoll), cetearyl glucoside, an acrylates/vinyl isodecanoate crosspolymer, an ammonium acryloyldimethyltaurate/VP copolymer and ethylene diamine tetraacetic acid; the component B comprises triethanolamine (TEA); the component C comprises panthenol, a CH collagen crosslinked polymer, beta-glucan, Xinlishi, SUCUS, Tridermol Hydrant, Waterin, phellinus linteus extracts, CalmYang, caprylhydroxamic acid and flavor essence. All the components in the formula have synergetic effects so that the product can achieve the effects of penetrating into pores and intensively moisturizing the skin; water is supplemented, the skin is moisturized, and the skin dryness phenomena caused by season, age, skin type and the like are effectively improved.

The invention provides a traditional Chinese medicine for treating tumor, reducing blood glucose and curing gout. The traditional Chinese medicine is prepared from, in parts by weight, 5-120 parts of phellinus pini, 5-120 parts of phellinus linteus, 5-120 parts of inonotus obliquus, 5-120 parts of ganoderma applanatum and 5-120 parts of ganoderma tropicum. The traditional Chinese medicine can reduce blood glucose, reduce the uric acid value of a patient suffering from gout and relieve gout symptoms, has the effects of supporting the healthy energy, preventing relapse, metastasis and other conditions on the patient suffering from tumor and has the effect of improving the quality of life of the patient suffering from tumor.

There is disclosed novel Phellinus linteus Yoo. This mushroom is identified as novel as analyzed by restriction fragment length polymorphism and by microbiological features. A polysaccharide substance isolated from the fruiting bodies or mycelia the strain and other Phellinus spp. has an anticancer activity by inciting the cytotoxicity of T-cells and the antibody forming potency of B-cells. Also, the polysaccharide substance shows a potent activity against cancer metastasis. The polysaccharide substance, which can stimulate the immunity of the body, may be effective in the prophylaxis and treatment of immune-related diseases, such as cancers and AIDS, and in the study on the basic mechanism of the diseases.

The invention relates to a preservation method of phellinus linteus. A main material of a culture medium is prepared from the following components by mass: 50% of tung tree wood dust, 15% of maple tree wood dust, 25% of wheat bran, and 10% of corn cob powder; an auxiliary material is prepared from the following components by mass: 16% of magnesium sulfate, 34% of potassium dihydrogen phosphate, and 50% of gypsum powder; a formula of the culture medium of a wood dust tube comprises the main material, the auxiliary material and water according to a ratio of 1:0.003:1.4. The preparation method comprises the following steps of firstly, uniformly mixing the two types of wood dust in the main material, sequentially adding the wheat bran and the water, and uniformly mixing; adding the mixed auxiliary material, uniformly mixing, adding water and uniformly stirring; separately loading the culture medium into test tubes, packaging, sterilizing, and inoculating, so as to obtain the phellinus linteus preservation strain when the phellinus linteus can well grow and be free from infection by infectious microbes after re-transfer verification. The preservation method has the advantages that the effect is excellent, and the preserved strain can be effectively preserved for 2 to 5 years; after activating, the growth rate of hypha, growth condition, biology conversion rate and the like are basically free from influence.

The invention discloses an oral rhizoma polygonati edible-fungus liquid for resisting fatigue and improving the immunity and a preparation method thereof. The oral liquid adopts pure natural food materials including myrtle fruits, hericium erinaceus, cordyceps sinensis, tremella, agaric, lentinus edodes, cordyceps militaris, poria cocos, polyporus umbellatus and phellinus linteus as raw materialsfor extraction and concentration to obtain an extractive solution, and then the extractive solution is mixed with rhizoma polygonati nano-powder and subjected to homogenization, sterilizing, encapsulation and the like to obtain the oral rhizoma polygonati edible-fungus liquid with the functions of improving the immunity and resisting fatigue. The oral liquid also has the effects of replenishing blood, invigorating qi, nourishing yin, invigorating the kidney, moistening the lung, promoting salivation and the like and has a wide application range. People of all ages and sexes can drink the oralliquid. The preparation method is simple, easy to operate and environmentally friendly and can be used for industrial production.

The invention discloses a traditional Chinese medicine hair-blacking and hair-smoothing agent. The agent is prepared from, by weight, 20-40 parts of radix rehmanniae preparata, 15-30 parts of radix polygonum multiflorum preparata, 22-30 parts of herba ecliptae, 20-30 parts of gynostemma pentaphyllum, 20-30 parts of fructus ligustri lucidi, 18-28 parts of rhizoma chuanxiong, 18-28 parts of angelicasinensis, 5-10 parts of dried ginger, 5-12 parts of meadowfoam seed oil, 6-12 parts of lophatheri herbak, 5-10 parts of phellinus linteus, 3-8 parts of ganoderma, 5-10 parts of Sarcodon imbricatus, 5-10 parts of arimillaria mellea, 6-12 parts of gulfweed and 5-15 parts of black mulberries. The invention further provides a preparation method of the traditional Chinese medicine hair-blacking and hair-smoothing agent. The traditional Chinese medicine hair-blacking and hair-smoothing agent has the good permeation promoting capacity, active ingredients in the product can reach deep skin tissue bymore rapidly permeating through fur and can provide adequate nutrients for the fur, metabolism is promoted, the product effect is better, and the effects of blacking hair, nourishing hair, protectinghair and achieving moisturizing and maintaining are achieved.