9 results about "Podophyllum" patented technology

The invention belongs to the technical field of novel composite medicines, and particularly relates to a polysaccharide composite nanoparticle adjuvant as well as a preparation method and application thereof. The polysaccharide composite nanoparticle adjuvant comprises mannose functionalized mesoporous silica nanoparticles and podophyllum hexandrum polysaccharide, and the podophyllum hexandrum polysaccharide is loaded in channels of the mannose functionalized mesoporous silica nanoparticles and on the surfaces of the mannose functionalized mesoporous silica nanoparticles. Nanoparticles are prepared through a solution gel method, carboxyl groups are contained on the surfaces of the nanoparticles through chemical denaturation, the nanoparticles and aldehyde groups on D-mannose are subjected to an esterification reaction under the action of a catalyst, mannose functionalized mesoporous nanoparticles are obtained, then polysaccharide is loaded on pore channels and the surfaces of the mesoporous nanoparticles, and construction of the nanoparticles is completed. The retention time and the action effect of the medicine in the body can be greatly improved, meanwhile, the toxic and side effects are reduced or avoided, and the polysaccharide nanoparticle adjuvant has the key and breakthrough points of targeting and autonomous targeting polysaccharide nanoparticle adjuvants.

The invention relates to the field of medicinal plant genetic engineering, and particularly discloses a CYP450 gene participating in lignan synthesis in a plant and a coding product thereof. The invention aims to analyze the oxidation modification step of the lignan biosynthetic pathway in the plant, and provides a gene element for the subsequent synthetic biological application. The invention discloses a C-5 hydroxylase gene sequence and an amino acid sequence thereof required by biosynthesis of aryl tetrahydronaphthalene lignans in podophyllum hexandrum for the first time. Reference is provided for subsequent rational design and directional transformation of lignan compound structure modification enzymes, development and utilization of biosynthetic pathway elements and synthetic biology heterologous efficient production of podophyllotoxin compounds.

The invention discloses a medical application of an isopentenyl flavonoid compound or an aryl naphthalene lignan compound in podophyllum hexandrum. The isopentenyl flavone compound and the aryl naphthalene lignan compound can obviously inhibit proliferation of FLS cells induced by TNF-alpha, and show anti-RA activity in a rat RA model induced by a complete Freund's adjuvant; the isopentenyl flavonoid compound can play an anti-RA effect by inhibiting JAK1, inhibit phosphorylation of proteins such as PLC-gamma, p38 and JNK in FLS cells induced by TNF-alpha, inhibit NF-kappa B intracellular expression, and inhibit a JAK1 downstream signal channel at the cellular level; the aryl naphthalene lignan compound can play an anti-RA effect by inhibiting TNF-alpha, inhibit expression of RIP-1 and TRAF2 / 4 / 5 in FLS cells induced by TNF-alpha, inhibit expression in NF-kB nucleus, and inhibit a TNF-alpha downstream signal channel at the cellular level.

The invention discloses traditional Chinese medicine granules for treating lung cancer and a preparation method thereof, and relates to the technical field of traditional Chinese medicine formulas. The invention discloses traditional Chinese medicine granules for treating lung cancer. The traditional Chinese medicine composition is prepared from aged black nightshade, marsdenia tenacissima, podophyllum, thunberg fritillary bulb, sculellaria barbata, oldenlandia diffusa, ganoderma applanatum, poria cocos, phellinus igniarius, honeysuckle, fructus forsythiae, dandelion, carbonized sanguisorba root, humifuse euphorbia herb, phyllanthus urinaria, folium isatidis, astragalus membranaceus, cottonrose hibiscus leaves, pericarpium trichosanthis, lance asiabell root, radix ranunculi ternati, spicate clerodendranthus herb, platycodon grandiflorum, aster, rhizoma atractylodis macrocephalae, herba cepbalanoplosis segeti, scorpio, turtle shell, radix ophiopogonis, codonopsis pilosula, radix pseudostellariae, momordica grosvenori, fig, Japanese ardisia herb and pyrrosia lingua. The traditional Chinese medicine has the effects of detoxifying, astringing to stop bleeding, astringing lung and improving symptoms, can finally reduce relapse, and is safe and reliable.

A method for improving germination rate of seeds of endangered plant podophyllum hexandrum comprises the following steps: step 1, after podophyllum hexandrum fruits are harvested in the same year, pulp and peel do not need to be peeled off, the podophyllum hexandrum fruits can be dried at low temperature after being dried in the shade, and the storage time is selected to be November; 2, the fruits are taken out from the end of January or the beginning of February of the next year, peel and pulp are peeled off, full seeds without plant diseases and insect pests and nicks are soaked with clear water, and then the seeds are soaked and disinfected with a 0.1% potassium permanganate solution; 3, soaking the swollen and disinfected seeds with a GA3 solution with the concentration of 100 mg / L, then taking out the seeds, and washing the seeds with clear water; and 4, uniformly placing the seeds on the laid filter paper by using tweezers, and carrying out germination operation to wait for germination. From low-temperature dry storage of podophyllum hexandrum fruits to final seed germination, the phenomenon of seed mildew and rot easily caused by poor control of the water content of sand in the sand storage process can be reduced, meanwhile, the germination rate of the podophyllum hexandrum can be effectively increased, and the method is worthy of popularization and application.

A method for extracting sesquiterpenoids from the stems and leaves of Arisaema heterophyllum and its application are disclosed. This method can effectively extract sesquiterpenoids G and I from the stems and leaves of Arisaema heterophyllum, effectively solving the problem of preparing drugs for the treatment of Alzheimer's disease. The preparation method is as follows: the dried stems and leaves of Arisaema heterophyllum are pulverized, heated under reflux, filtered, concentrated under reduced pressure, dissolved in water, and extracted sequentially with petroleum ether, ethyl acetate, and n-butanol, respectively, and then concentrated and dried. The concentrated and dried ethyl acetate fraction is eluted, the fraction retained for the retention time is collected, concentrated and dried to obtain the final product. This method is easy to operate, scientifically sound, and can effectively extract sesquiterpenoids G and I from the stems and leaves of Arisaema heterophyllum. These compounds can significantly improve Aβ25-35-induced PC-12 cell damage, opening up new uses for the active ingredients in the stems and leaves of Arisaema heterophyllum, improving the commercial and industrial value of Arisaema heterophyllum, and simultaneously opening up new avenues for the treatment of Alzheimer's disease, resulting in significant economic and social benefits.

The present application relates to the technical field of podophyllotoxin extraction, and particularly relates to a podophyllotoxin separation and purification device, which comprises a juice pressing mechanism, and the juice pressing mechanism is used for juice pressing treatment of chopped rhizomes and leaves. In the present application, the pressing root disc rotates in the positioning ring when descending, so that the arc-shaped cutter at the bottom of the pressing root disc can cut the plant rhizomes in two sections when extruding the plant rhizomes, facilitating the re-chopping of the uncut rhizomes, thereby facilitating the improvement of the juice pressing extraction effect of the plant rhizomes. The above operation can simultaneously perform the juice pressing extraction of medicinal juice of the plant leaves and rhizomes of the podophyllum genus, the obtained medicinal liquid can separate the podophyllotoxin through the decocting method, the medicinal liquid obtained through the juice pressing has less impurities, so that the medicinal substances obtained after decocting can be conveniently filtered, the separation speed of the podophyllotoxin is accelerated, and the medicinal substances in the plant leaves and rhizomes can be simultaneously extracted through the juice pressing mechanism, so that the waste of effective medicinal resources is avoided.

The invention discloses a traditional Chinese medicine composition for treating thyroid cancer as well as a preparation method and application thereof, and relates to the technical field of traditional Chinese medicine formulas. The traditional Chinese medicine composition for treating thyroid cancer comprises aged black nightshade, marsdenia tenacissima, podophyllum, thunberg fritillary bulb, sculellaria barbata, radix bupleuri, fried gardenia, cortex moutan, angelica sinensis, ligusticum wallichii, poria cocos, radix curcumae, radix ranunculi ternati, radix semiaquilegiae, blackberry lily, wild buckwheat, rhizoma smilacis glabrae, rhizoma dioscoreae nipponicae, rhizoma cyperi, radix paeoniae alba, salvia chinensis, salvia miltiorrhiza, plantain herb, burdock and polygala tenuifolia. And the traditional Chinese medicine composition is safe and reliable.

The invention discloses traditional Chinese medicine granules for treating liver cancer and a preparation method thereof, and relates to the technical field of traditional Chinese medicine formulas. The traditional Chinese medicine granules for treating liver cancer are prepared from aged black nightshade, marsdenia tenacissima, podophyllum, thunberg fritillary bulb, sculellaria barbata, oldenlandia diffusa, lucid ganoderma, phellinus igniarius, polygonum cuspidatum, sedum sarmentosum, lysimachia christinae hance, hypericum japonicum, dandelion, roasted turtle shell, astragalus membranaceus, ginger processed pinellia, scorpio and reed rhizome. The traditional Chinese medicine composition is composed of sargentgloryvine stem, patrinia heterophylla, codonopsis pilosula, Chinese yam, bighead atractylodes rhizome, fried endothelium corneum gigeriae galli, curcuma zedoary, radix curcumae, inula flower, achyranthes root, Chinese polyphaga, honeycomb, processed rhizoma cyperi, radix ophiopogonis, fig and silybum marianum, has the effects of detoxifying and improving symptoms, can finally reduce relapse, and is safe and reliable.