89 results about "Podophyllum" patented technology

The present invention describes a method for inducing improved seed germination in a high altitude medicinal plant, Podophyllum hexandrum Royle through hot water treatment at 40° C.-100° C. for 30-120 seconds.

The invention discloses nitrogen substitution podophyllum type derivatives with antitumor activity and a preparation method and application thereof. The nitrogen substitution podophyllum type derivatives (as formula V) with fine antitumor activity are prepared by introducing 1-(3-amino propyl) imidazole, benzimidazole, 2-thienylmethylamine or trihydroxymethyl aminomethane to C ring 4-position activated by 4-demethylation surface podophyllotoxin. The nitrogen substitution podophyllum type derivatives can act on tumor cells with multiple paths and multiple target points, and accordingly better antitumor curative effect is achieved. Antitumor activity of the derivatives is evidently increased compared with that of the 4-demethylation surface podophyllotoxin. The derivatives can be prepared into antitumor drugs to apply to antitumor therapies.

The invention provides a method for preparing high-purity podophyllotoxin from Chinese podophyllum root, consisting of two parts of: extraction of alcohols used for raw materials to obtain extractum and extraction of the extractum to obtain podophyllotoxin crude product, and high-pressure column chromatography of the podophyllotoxin crude product. The method comprises the following specific steps of: after superfine pulverizing rootstalks of the Chinese podophyllum root, heating and extracting the pulverized rootstalks by using alcoholic solvents under the ultrasonic condition for three times, merging extracting solutions in the three times, and concentrating the alcoholic solvents by the merged extracting solutions to obtain red brown extractum with the specific gravity of 1.0-1.2; then adding water with the volume of 1-2 times of that of the extractum to obtain alcohol-water phase of podophyllotoxin; extracting the alcohol-water phase of podophyllotoxin by using methylene dichloride with the volume equal to that of the alcohol-water phase of podophyllotoxin for three times, merging methylene dichloride phases in the three times, and concentrating to obtain yellowish podophyllotoxin crude product with the purity of 40% to 50%; and dissolving the yellowish podophyllotoxin crude product, loading sample to a high-pressure column with silica gel of 300-400 meshes, eluting by using mixed eluent of acetone + methylene dichloride solvent, collecting eluent of product sections, concentrating, filtering, and drying to obtain the podophyllotoxin fine product with the purity of more than 99%.

The invention discloses a podophyllum hexandrum tissue culture seedling and exercising seedling transplanting acclimatization method. The podophyllum hexandrum tissue culture seedling and exercising seedling transplanting acclimatization method comprises the steps of bottle-opening seedling exercising of podophyllum hexandrum tissue culture seedlings and young seedlings in a cultivation chamber, transplanting matrix seedling exercising, greenhouse transplanting, management after transplanting, field acclimatization and the like. The method can improve transplanting survival rate of the podophyllum hexandrum tissue culture seedlings by above 15%, podophyllum hexandrum plants survived after field acclimatization grow robustly, and main leaves can reach 4-5. Compared with field artificial planting plants with the same growth age limit, the main leaves are 2-3 more than those of the field artificial planting plants, roots are thick and solid, and the number of the roots are more.

The present invention relates to the extraction and purification technology of podophyllotoxin via solvent crystallization process and compression chromatographic process. The present invention has simple operation, is easy to use in industrial production to obtain podophyllotoxin with purity not lower than 97 %. Podophyllotoxin may be extracted directly from Chinese podophyllum root or may be obtained from refining the extract of Chinese podophyllum root.

The invention discloses a sulfur-substituted podophyllum derivative, and a synthetic method and application thereof. Through sulfur substitution reaction, 2-mercapto-1,3,4-thiadiazole or 3-mercapto-1,2,4-triazole is respectively introduced into a C-4 site of podophyllotoxin or 4'-demethyl-epipodophyllotoxin so as to obtain the sulfur-substituted podophyllum derivative represented by a formula (V). Cell apoptosis test results of in-vitro BGC823, HepG2 and A549 tumor cell strains show that sulfur-substituted podophyllum derivative has good anti-tumor activity, high safety and low toxicity and can be prepared into clinical anticancer medicines.

The invention discloses a medicine for treating occupational chronic lead poisoning caused by spleen-stomach disharmony and a preparation method. The medicine comprises the following medicinal materials: valeriana jatamansi, uraria crinita, mentha spicata, camphorwood seed, eupatorium japonicum, gnaphalium tranzschelii, bushy dasiphora flower, diuranthera major, dracontomelon duperreanum, desmodium triquetrum, dendrobe, arisaema intermedium blume, helwingia root, podophyllum hexandrum, polygonum chinense, adiantum capillus-veneris, lithospermum, garden balsam stem, fructus polygoni orientalis, kamuning, herba pyrolae, peperomia blanda, ternate buttercup root, marsdenia tinctoria, acmella ciliate, indian lettuce, creeping violet herb, centella, nude pronephrium rhizome, ovateleaf holly bark and liquorice. According to the medicine disclosed by the invention, the traditional Chinese medicinal materials with different medicine properties are scientifically combined to achieve the effects of detoxifying, regulating vital energy, regulating the middle warmer, calming the adverse-rising energy, accelerating poison discharge and treating both symptoms and root causes; the medicine is safe, effective, relatively few in adverse effects and relatively short in treatment course; the preparation technology of the medicine is simple, convenient and low in cost; and the occupational chronic lead poisoning is not easy to recur after being cured.

The invention discloses a sulfur-substituted podophyllum derivative and a bioconversion, separation and purification method thereof. The sulfur-substituted podophyllum derivative with antitumor activity, shown as Formula (I) or (II), is obtained by modifying the molecular structure of podophyllotoxin or 4'-demethylepipodophyllotoxin through a bioconversion method. Preliminary study on in-vitro tumor cytotoxicity shows that the sulfur-substituted podophyllum derivative disclosed by the invention has stronger inhabitation action on the growth of tumor cells such as BGC823, HepG2, A549 and the like, and thus can be used as an antitumor compound to achieve a potential drug development value. According to the invention, the podophyllotoxin and the 4'-demethylepipodophyllotoxin are converted into the sulfur-substituted podophyllum derivative by using a bioconversion method, and therefore, the sulfur-substituted podophyllum derivative has the advantages of low cost and E-factor, simplicity in operation, easiness for culturing, controllable quality, capability of effectively preventing chemical residuals and the like.

The invention provides an external application traditional Chinese drug for treating psoriasis vulgaris. The external application traditional Chinese drug is a agent prepared from the following raw materials, by weight, 5-9 parts of densefruit pittany root bark, 5-9 parts of japanese ampelopsis root tuber, 5-9 parts of lightyellow sophora root, 9-15 parts of fructus kochiae, 5-9 parts of pinellia, 2-5 parts of amur corktree bark, 3-9 parts of common cnidium fruit, 1-5 parts of alum, 6-9 parts of stiff silkworm, 2-4 parts of chaulmoogratree seed, 9-15 parts of dried alum, 6-12 parts of mirabilite, 10-30 parts of wild buckwheat rhizome, 1-4 parts of glaber herba sarcandrae inj, 3-9 parts of japanese pogodatree flower, 2-6 parts of kansu euphorbia root, 9-15 parts of sulphur, 6-9 parts of kaempferia galanga, 3-9 parts of pomegranata rind, 6-15 parts of common bletilla tuber, 3-9 parts of podophyllum, 3-9 parts of siberian cocklebur fruit, 6-12 parts of smoked plum, 9-15 parts of flatspine pricklyash, 6-9 parts of psoralea corylifolia, 3-9 parts of common camptotheca fruit, 9-15 parts of Chinese soapberry fruit, and 1-30 parts of Chinese azalea flower. According to the traditional Chinese drug preparation of the present invention, the traditional Chinese drug five element theory is adopted, the medicine is applied aiming at the symptoms according to the psoriasis vulgaris etiopathogenisis, and the external application traditional Chinese drug has effects of heat clearing, detoxification, blood activating, and collateral dredging, and is suitable for more than 99% psoriasis vulgaris patients.

The invention relates to a radiationproof Chinese herbal preparation for the ultrasound department and a preparation method of the radiationproof Chinese herbal preparation. The Chinese herbal preparation comprises the following raw materials of effective components: sesame, radix angelicae pubescentis, papaya, houttuynia cordata, acanthopanax, semen cassia, podophyllum, lucid ganoderma, tremella fuciformis, spirulina, gingko, day lily, centella, seabuckthorn, lycium barbarum and ginseng. The radiationproof Chinese herbal preparation is a pure Chinese herbal preparation prepared from medicinal materials with proper compatibility by using the modern advanced pharmaceutical technology, has the effects of clearing away heat and toxic materials and smoothing liver and gallbladder, has favorable treating effects on symptoms such as fatigue, lassitude, neurasthenia and the like induced by the fact that an ultrasonic worker works on an ultrasonic job for a long term, and is exact in medicinal effect, short in treatment course, high in effect taking speed and free of toxic or side effect.

The invention discloses an acidamide substitution podophyllum derivative with antineoplastic activity and a preparation method and application thereof. According to the invention, 2-pyrazine pyrazine, 5-methyl-2-pyrazine pyrazine, theophylline acetic acid and acetic acid are subjected to acidamide substitution reaction with 4'-demethylepipodophyllotoxin, so that the acidamide substitution podophyllum derivative with the antineoplastic activity, expressed by the formula (V), can be obtained. The acidamide substitution podophyllum derivative acts on cancer cells by means of multiple ways and multiple target points, and thus has better antineoplastic efficacy. As indicated by the in vitro cell activity inhibition test, the acidamide substitution podophyllum derivative disclosed by the invention has excellent antineoplastic activity and low toxic and side effects, and can be made into antineoplastic drugs for clinic antineoplastic treatment.

The invention discloses an aspergillus fumigatus which can produce podophyllotoxin, epipodophyllotoxin biglucoside and astragaloside, and application thereof. The aspergillus fumigatus TEQA CGMCC No. 3115 has the function of producing podophyllotoxin, epipodophyllotoxin biglucoside and flavonoids astragaloside which have the physiological property the same as that of Chinese podophyllum root. The aspergillus fumigatus can be used for preparing podophyllotoxin and astragaloside. The method for preparing podophyllotoxin, epipodophyllotoxin biglucoside and astragaloside has the advantages of simple fermentation conditions, easy bacterium culture and short production period, with potential application for industrialized mass production. Besides, the aspergillus fumigatus provides a new way for resource development of podophyllotoxin and has important meaning to protect ecological diversity of plants.

The invention discloses an esterified podophyllum derivative with antitumor activity and a preparation method and the usage of the esterified podophyllum derivative. The esterified podophyllum derivative with antitumor activity and shown as a formula (V) is obtained by means of esterification reaction of adamantine formic acid, orotic acid, 4-(pyrimidine sulfo) phenylacetic acid, cyclohexane carboxylic acid, 3,4-dimethoxy benzoic acid, 2-butoxycarbonyl-3-phenylalanine or 4-methoxy phenylacetic acid with podophyllotoxin or 4'-demethyl-epipodophyllotoxin. The esterified podophyllum derivative has multi-way multi-target actions on tumor cells to achieve better antitumor curative effects. According to in-vitro cell viability inhibition tests, the esterified podophyllum derivative has excellent antitumor activity and low toxic and side effects and can be prepared into antitumor drugs to be applied to antitumor treatment.

Provided is a winter seedling culture technology for the Tibetan herb podophyllum hexandrum Royle. The technology includes the concrete steps of firstly, selecting seeds; secondly, determining the sowing period and the sowing environment; thirdly, soaking the complete and dry seeds with good maturity in a 5 mol / L gibberellin water solution for 24-36 h, sowing the seeds in seedling culture pots with rich humus soil, and then placing the seedling culture pots with the seeds in soil neatly, wherein two seeds are sown in each pot, and the sowing depth ranges from 3 cm to 4 cm; fourthly, performing field management on seedlings; fifthly, transplanting the seedlings, wherein the seedling culture pots are sheared to be broken after the overground parts of the podophyllum hexandrum Royle turn yellow and wither in late autumn, and podophyllum hexandrum Royle roots with soil are cultivated in soil good in light transmittance and rich in humus in a place with the altitude ranging from 2200 m to 3400 m and the annual precipitation ranging from 500 mm to 800 mm. By means of the technology, the germination rate of the seeds is larger than 96%, the germination potential is larger than 85%, the seedlings grow healthily and strongly, the root number reaches 2 or 3 in the transplanting process, and the root length reaches 18 cm.

The invention discloses a Tibetan medicine composition, which comprises the following raw materials by weight: 10-20 parts of gymnadnia conopsear broyn, 5-15 parts of Saussurea medusa, 5-15 parts of Podophyllum hexandrum and 5-15 parts of lithospermum. The invention also discloses a preparation method and an application of the Tibetan medicine composition. The Tibetan medicine composition can be taken as an efficacy component to be added in a cosmetic system and can substitute an antiseptic in a matrix for bacteriostasis and anticorrosion, the Tibetan medicine composition is safe and has no stimulation to sensitive skin, and has the efficacy of whitening, spot removing and sun protection.

The invention discloses an esterified podophyllum derivative with antineoplastic activity and a preparation method and application thereof. According to the invention, 2-pyrazine pyrazine, 5-methyl-2-pyrazine pyrazine, theophylline acetic acid, fluoroquinolone acid and 2-quinoline carboxylic acid are subjected to esterification reaction with 4'-demethylepipodophyllotoxin, so that the esterified podophyllum derivative with the antineoplastic activity, expressed by the formula (V), can be obtained. The esterified podophyllum derivative acts on cancer cells by means of multiple ways and multiple target points, and thus has better antineoplastic efficacy. As indicated by the in vitro cell activity inhibition test, the esterified podophyllum derivative disclosed by the invention has excellent antineoplastic activity and low toxic and side effects, and can be made into antineoplastic drugs for clinic antineoplastic treatment.

The invention discloses a Chinese herb additive for Chinese shrimp feed. The Chinese herb additive comprises, by weight, 12-20 parts of roots of straight ladybell, 10-20 parts of roots of paniculate swallowwort, 10-20 parts of Chinese incarvilla herbs, 5-15 parts of fructus viticis, 4-12 parts of usnea longissima ach, 3-9 parts of chain fern, 10-20 parts of hawksbill, 10-20 parts of mantis, 6-18 parts of ranunculus ternatus thunb, 5-13 parts of podophyllum, 4-12 parts of ephedra sinica, 6-8 parts of alpinia oxyphylla, 5-15 parts of panax zingiberensis and 5-10 parts of canna. The invention further discloses Chinese shrimp feed containing the Chinese herb additive and a preparation method thereof. The Chinese herb additive in the feed can be used for improving the immunity of Chinese shrimps, preventing the Chinese shrimp from suffering from viral diseases and preventing the yield from being lowered; in addition, sweet potato vines, clamshell powder and pine needle meal in the feed can additionally supplement microelements and nutrients needed by the Chinese shrimps.

The invention relates to oral liquid for preventing puerperal fever and belongs to the technical field of traditional Chinese medicines. The oral liquid for preventing puerperal fever is prepared from the following raw materials in parts by weight: 8-12 parts of impatiens balsamina, 5-10 parts of winged euonymus twig, 8-12 parts of Artemisia indica, 5-8 parts of podophyllum, 3-8 parts of radix et rhizome rubiae, 5-10 parts of seed of pomegranate, 5-10 parts of root of Onosma paniculatum, 6-12 parts of cinnamon, 8-12 parts of Corydalis pygmaea, 5-10 parts of veronica eriogyne, 3-8 parts of caulis spatholobi, 3-8 parts of halite and 2-4 parts of sal ammoniac. According to the oral liquid for preventing puerperal fever, the various traditional Chinese medicine components are reasonable in compatibility, the effects of dispelling wind and eliminating dampness, dredging wind cold, warming main and collateral channels and replenishing blood and regulating vital energy can be achieved, and the puerperal fever is greatly reduced, so that the probability of rheumatism, rheumatoid and other postpartum complications caused by the puerperal fever is greatly reduced.

The invention discloses a brewing method of date palm and polygonatum odoratum sparkling wine. According to the method, fresh date palms and polygonatum odoratum are adopted as raw materials, and fruit wine is obtained through raw material pretreatment, mixing, fermentation, bottling, secondary fermentation, bottle transferring, residual removing and packaging; traditional Chinese medicine ingredients including gentiana purdomii marq, silkworm larva, silkworm larva, podophyllum hexandrum, decaisne fargesii, phlomis younghusbandii, astragalus membranaceus, cortex berberidis, walnut kernels, Chinese yam, balanophora harlandii, donkey-hide gelatin, liquorice and the like are added, the nutritive value of the date palms and the polygonatum odoratum is fully utilized, and the utilized raw materials and the added traditional Chinese medicine ingredients achieve a synergistic effect, so that the fruit wine has the nutritive ingredients of the raw materials as well as the health-care effects of moistening the lung for removing phlegm and relieving a cough and promoting the secretion of saliva or body fluid, is beneficial to the body health, and a preparation method is simple, feasible and high in operability; due to the fact that the finished product fruit wine is subjected to secondary fermentation in bottles, the wine body can contain a great amount of carbon dioxide, the luster is bright and smooth, rich and fine foam is achieved, the wine body is fresh and soft sweet in taste and has delicate fragrance, the wine fragrance and the fruit fragrance coexist, and remarkable economic benefits are achieved.

The invention relates to the technical field of plant planting, in particular to a method for cultivating seedlings of podophyllum hexandrum. The technical scheme comprises seed collection and harvest, seed treatment, seedling cultivating field selection, field preparation, sowing, field management and transplanting. According to the method for cultivating the seedlings of podophyllum hexandrum, seeds of podophyllum hexandrum are stored in low temperature sand so as to break the dormancy of the seeds of podophyllum hexandrum, and the germination rate of the seeds is improved. The germination rate of the seeds of podophyllum hexandrum treated by the method for cultivating the seedlings of podophyllum hexandrum is up to 95% or above, and the survival rate of the seedlings of podophyllum hexandrum reaches 90% or above.

The invention discloses a method for transforming podophyllotoxin into 4-(2,3,5,6-tetramethylpyrazine-1-group)-4'-demethylepipodophyllotoxin, comprising the following steps of: (1) adding podophyllotoxin into a liquid fermentation medium, and carrying out biotransformation by inoculating second-class liquid seeds of humic soil fungi; (2) separating and purifying the product from the Step (1), followed by adding into the liquid fermentation medium, carrying out biotransformation by inoculating second-class liquid seeds of podophyllum taxa endophytes; then continuously carrying out biotransformation by adding ligustrazine, and collecting the transformation product. The invention also discloses a method for purifying 4-(2,3,5,6-tetramethylpyrazine-1-group)-4'-demethylepipodophyllotoxin from the above product and a method for detecting the content of 4-(2,3,5,6-tetramethylpyrazine-1-group)-4'-demethylepipodophyllotoxin. According to the invention, the molecular structure of podophyllotoxin is modified by the biotransformation method, which has advantages of mild reaction condition, low E-factor, quality controllability and simple separation process.

The invention discloses a method for preparing Tibetan medicine for treating hemorrhoids. The Tibetan medicine is prepared from active ingredients prepared from the following components according to a certain weight ratio by a mixed crushing method or aqueous extraction and alcohol precipitation method or an alcohol extraction and aqueous precipitation method: dendrobe, podophyllum hexandrum, wallichiana, Tibetan madder, cremanthodium reniforme, borax, lac, whin, procumbent speedwell, androsace umbellata and myricaria germanica, and the Tibetan medicine can be prepared into any common oral dosage forms. The Tibetan medicine has the functions of killing insects, diminishing inflammation and promoting blood circulation to remove blood stasis. The Tibetan medicine is used for treating hemorrhoids, slight pain, stabbing pain, burning pain, swelling pain, itching bleeding of anus and skin around the anus, defecation haemorrhage, perianal pain and the like.

The invention relates to the technical field of traditional Chinese medicinal liquor, and particularly relates to liquor containing ginseng and cornua cervi pantotrichum and a preparation method thereof. The liquor is prepared from ginseng with effects of tonifying vital energy, restoring pulse, preventing exhaustion, cornua cervi pantotrichum and blood thereof for tonifying kidneys, tonifying Yang, promoting essence, nourishing blood, supplementing marrow and strengthening bones, along with podophyllum for eliminating stagnation, removing stasis and removing toxicity, obscured homalomena rhizome for strengthening tendons and bones, parasitic loranthus for tonifying liver and kidneys and strengthening tendons and bones, Chinese wolfberry fruits for nourishing liver and kidneys, replenishing vital essence to improve eyesight, Chinese wolfberry leaves for tonifying deficiency, boosting essence, promoting generation of body fluid and tonifying liver, and is added with herba epimedii, lily, Chinese dates, indigo naturalis and fructus rubi to regulate medicinal property. The medicinal materials are combined to achieve the effects of boosting essence, nourishing blood, tonifying Yang and strengthening bones, can be used for nourishing Yang, nourishing yin and relieving toxin of liquor. By adopting ultrasonic treated cornua cervi pantotrichum and blood thereof, tissues and erythrocyte can be crushed, soluble substances can be increased, and nutritional substances can be utilized sufficiently. Finally the liquor containing cornua cervi pantotrichum has rich nutrition, can be used for strengthening tendons and bones, relieving fatigue, tonifying Yang and nourishing yin, and cannot easily causes internal fire if being taken for more.

The invention discloses an aspergillus fumigatus which can produce podophyllotoxin, epipodophyllotoxin biglucoside and astragaloside, and application thereof. The aspergillus fumigatus TEQA CGMCC No. 3115 has the function of producing podophyllotoxin, epipodophyllotoxin biglucoside and flavonoids astragaloside which have the physiological property the same as that of Chinese podophyllum root. Theaspergillus fumigatus can be used for preparing podophyllotoxin and astragaloside. The method for preparing podophyllotoxin, epipodophyllotoxin biglucoside and astragaloside has the advantages of simple fermentation conditions, easy bacterium culture and short production period, with potential application for industrialized mass production. Besides, the aspergillus fumigatus provides a new way forresource development of podophyllotoxin and has important meaning to protect ecological diversity of plants.

The invention discloses an imitative-ecological podophyllum hexandrum seedling raising method, belongs to the field of ecological planting of medicinal plants and aims at solving the problems of low seedling raising rate, slow seedling growth, long seedling raising period, high cost and incidence rate increase of root rot, damping off and other diseases at a seedling stage of an existing method. The method comprises the steps of seed harvesting and storage, seed germination accelerating, sowing in a greenhouse holed tray, young seedling growth promotion and prevention and control of the diseases at the seedling stage, early sowing, planting and transplantation of podophyllum hexandrum holed-tray seedlings and culture and harvesting of high-quality podophyllum hexandrum seedlings. By adopting the imitative-ecological podophyllum hexandrum seedling raising method, the most suitable environment and nutrition conditions are created at different growth and development stages, the physiological conditions of the seedlings are improved, the seedling raising rate of the method is improved, seedling raising time is shortened, high-quality strong seedlings are cultured, further the reviving rate in a next year is improved, growth and development of the seedlings are promoted, the incidence rate is reduced, the cost is reduced, and the productivity is improved through intensive measures to achieve the best seedling raising effect.

The invention relates to the technical field of traditional Chinese medicine wine, and in particular, relates to a fresh deer blood wine and a preparation method thereof. The fresh deer blood wine gives full play to a role of deer blood in nourishing essence and blood and roles of deer bones in tonifying deficiency and strengthening bones and muscles, is supplemented by podophyllum for eliminatingstagnation and dispersing blood stasis and detoxifying and hgriopsis cumingii meat for nourishing yin and clearing heat, and is also combined with associated effect initiating of glossy privet fruit,radix polygonati officinalis, herba epimedii radix scrophulariae, indian kalimeris herb, radix adenophorae, cornus officinalis and radix achyranthis bidentatae, and various drugs are combined to achieve the effect of nourishing both qi and blood, nourishing yang and nourishing yin, avoids vigorous deficiency fire, and removes the poison of alcohol; the fresh deer blood is subjected to ultrasonictreatment, erythrocytes are disintegrated, dissolution substances are increased, the nutrients of the fresh deer blood are more fully utilized, and the fresh deer blood wine rich in nutrients, capableof nourishing both qi and blood and nourishing yin and yang is ultimately obtained.