28 results about "Podophyllotoxin" patented technology

The application belongs to the technical field of animal models for basic scientific research, and particularly relates to a method for establishing an acute lung injury animal model, which comprises the following steps: sending a podophyllotoxin or a podophyllotoxin reagent into a rat body through oral administration or intragastric perfusion to obtain the acute lung injury animal model. The method for establishing the acute lung injury animal model provided by the application uses the podophyllotoxin as an inducer for establishing a mouse acute lung injury model, and the mouse can orally take or intragastrically perfuse the podophyllotoxin to achieve the method, without needing a special medicine injection device, so that the method is simple in medicine taking, few in experimental steps, and does not need complex surgical operations.

The invention relates to the technical field of centrifugal devices, in particular to a centrifugal device for podophyllotoxin extraction.The centrifugal device comprises a fixing frame, a light-phase liquid collecting ring, a heavy-phase liquid collecting ring, a liquid inlet core rod, a rotary drum assembly and a driving motor, supporting seats are fixedly connected to the two ends of the top face of the fixing frame, and a U-shaped frame is fixedly connected between the two supporting seats; the rotary drum assembly is rotationally connected to the fixing frame and comprises a bottom plate, sealing parts are fixedly connected to the bottom plate in an annular equal-angle mode, and a top cover part is fixedly connected to the top ends of the multiple sealing parts. According to the centrifugal machine, through the arrangement of the rotary drum assembly and the movable plate, when the viscosity of mixed liquid is large, the inclination angle of the movable plate can be adjusted, so that the gradient of the movable plate is reduced, the mixed liquid conveniently flows and climbs upwards along the movable plate during centrifugation, and meanwhile, the top end of the movable plate expands outwards, so that the centrifugal force of the mixed liquid on the outer edge of the top end of the centrifugal cavity is increased; and therefore, the mixed liquid with relatively high viscosity can be conveniently centrifuged, and can be conveniently adjusted according to the viscosity of the mixed liquid during multiple times of centrifugation.

A whitening composition containing the following components: Mori folium, Panacis quinquefolii radix, (-)-podophyllotoxin, and (+)-glaucarubinone. Further provided is the use of the whitening composition in the preparation of a product for whitening the skin, or a product for inhibiting melanin synthesis, or a product for improving skin pigmentation.

The invention discloses a podophyllotoxin extraction management system based on the Internet of Things, and particularly relates to the field of the Internet of Things, the podophyllotoxin extraction management system comprises a management equipment arrangement module, an environment monitoring module, an extraction environment analysis module, an abnormal environment judgment module, an extraction sensing module, an extraction purity evaluation module and an extraction management module. The extraction purity is monitored through the management equipment when podophyllotoxin is extracted, so that an extraction environment is divided into an abnormal area and a normal area, the extraction environment is flexibly adjusted through the extraction terminal, the change condition of the extraction purity is obtained, the extraction quality of podophyllotoxin is evaluated, the optimal extraction time is determined, and the podophyllotoxin extraction efficiency is improved. On one hand, by determining the optimal extraction time, the yield of podophyllotoxin is maximized, and the waste of raw materials is avoided, on the other hand, the method is not limited to manual inspection, the extraction process is intelligently monitored, extraction parameters are adjusted in real time, the stability of the extraction process is ensured, and reference is provided for subsequent extraction of podophyllotoxin.

The application discloses a class of podophyllotoxin triazole ether derivatives, a preparation method and application thereof, belongs to the technical field of chemical pharmacy, and particularly relates to a class of podophyllotoxin triazole derivatives and application thereof in preparation of antitumor drugs. The application combines a triazole structure with a podophyllotoxin parent body by using a click chemistry reaction, designs and synthesizes a series of novel 1,2,3-triazole podophyllotoxin derivatives, optimizes the kinetic characteristics of the podophyllotoxin by introducing the triazole structure, and improves the anticancer effect and drug property. Molecular docking shows that the series of compounds have strong binding energy on AKT1 and tubulin, can target inhibit tubulin / AKT1, and in-vitro antitumor activity research shows that the molecules have strong inhibitory activity on the proliferation of human colon cancer cell strains, and can provide a new strategy for structural derivatization of podophyllotoxin.

The present invention relates to the technical field of centrifugal devices, and particularly relates to a centrifugal device for extracting podophyllotoxin, which includes a fixed frame, a light-phase liquid collection ring, a heavy-phase liquid collection ring, a liquid inlet core rod, a drum assembly, and a driving motor. Both ends of the top surface of the fixed frame are fixedly connected with support seats, and a U-shaped frame is fixedly connected between the two support seats. The drum assembly is rotatably connected to the fixed frame. The drum assembly includes a bottom plate, and closed parts are fixedly connected to the bottom plate in an annular and equiangular manner. The tops of multiple closed parts are fixedly connected with top cover parts. In the present invention, through the setting of the drum assembly and the movable plate, when the viscosity of the mixed liquid is relatively high, the inclination angle of the movable plate can be adjusted to reduce the slope of the movable plate, facilitating the upward flow and climbing of the mixed liquid along the movable plate during centrifugation. At the same time, the top of the movable plate expands outward, increasing the centrifugal force of the mixed liquid at the outer edge of the top of the centrifugal chamber, thereby facilitating the centrifugation of the mixed liquid with a relatively high viscosity and facilitating adjustment according to the viscosity of the mixed liquid during multiple centrifugations.

This invention discloses a data analysis-based monitoring system for the podophyllotoxin extraction process, specifically relating to the pharmaceutical field. The system includes a podophyllotoxin extraction data acquisition module, a podophyllotoxin extraction parameter monitoring module, a data preprocessing module, an impact data analysis module, an anomaly diagnosis module, a process optimization module, and a historical data storage module. The data analysis-based podophyllotoxin extraction process monitoring system completes the task of collecting and transmitting the first basic data during the podophyllotoxin extraction process through the podophyllotoxin extraction data acquisition module, providing a solid data foundation. Through the impact data analysis module, it obtains the second extraction effect, accurately grasping the degree of influence of each parameter during the extraction process on the final extraction effect, providing a quantitative basis for the extraction effect. Through the process optimization module, it obtains process optimization parameters, allowing for rapid and accurate adjustment of process parameters based on actual production conditions to achieve the best production results.

The invention belongs to the field of basic medical research, and particularly relates to a testicular toxicity animal model establishing method which comprises the following steps: performing intragastric administration on male rats with a preparation containing podophyllotoxin according to the dosage of 15 mg / kg per day; the medicine is continuously administrated for 4 days; according to the method, the rat can be induced to generate testicular toxicity phenotypes, and the phenotypes at least include that the number of sperms is remarkably reduced, the activity of the sperms is reduced, the testosterone level of serum is abnormally increased, and spermatogenic tubules in testicular tissue are irregular in shape and spermatogenic epithelium is thinned.

The invention provides a biotin-podophyllotoxin conjugate and a preparation method and application of a clathrate compound, and relates to the field of cancer treatment. The preparation method comprises the following steps: reacting-COOH of biotin with-OH or-NH2 of a connexon through acylation reaction, and coupling with-OH of podophyllotoxin, so as to prepare a plurality of biotin-podophyllotoxin conjugates, and preparing the biotin-podophyllotoxin conjugate inclusion compounds from the biotin-podophyllotoxin conjugates by using a solvent evaporation method. The reaction conditions are mild, the prepared biotin-podophyllotoxin conjugate and clathrate have specific reductive release or oxidative release characteristics in tumor cells, the targeting of podophyllotoxin is enhanced, the uptake rate of podophyllotoxin by tumor cells is enhanced, the toxicity of podophyllotoxin to tumor cells is improved, and the biotin-podophyllotoxin conjugate and clathrate have broad application prospects. Therefore, the compound has a wide application prospect in the field of anti-tumor treatment.

The present invention discloses a podophyllotoxin extraction management system based on the Internet of Things, which specifically relates to the field of the Internet of Things, including a management equipment layout module, an environmental monitoring module, an extraction environment analysis module, an abnormal environment judgment module, an extraction perception module, an extraction purity assessment module, and an extraction management module. The extraction purity is monitored by the management equipment during podophyllotoxin extraction, thereby dividing the extraction environment into abnormal areas and normal areas. The extraction environment is flexibly adjusted by the extraction terminal, and the change in extraction purity is obtained. The extraction quality of podophyllotoxin is evaluated, thereby determining the optimal extraction time. On the one hand, by determining the optimal extraction time, the yield of podophyllotoxin is maximized, avoiding the waste of raw materials. On the other hand, it is not limited to manual inspections, and the extraction process is intelligently monitored, and the extraction parameters are adjusted in real time to ensure the stability of the extraction process, while providing a reference for subsequent podophyllotoxin extraction.

The present invention discovers a compound capable of promoting proteasome activation contained in a Juniperus persica extract, and provides cosmetics, foods, pharmaceuticals and the like and / or raw materials incorporated therein for activating proteasomes in cells such as human skin cells using the compound to create a beautiful appearance. A proteasome activator containing, as an active ingredient, one or more compounds represented by formula (I) or formula (II) or a modified body thereof. (In the formula, R1 and R2 each independently represent a hydrogen atom, a hydroxyl group, a methoxy group, an acetoxy group, or a glucosyl group, and R3 each independently represent a hydrogen atom, a hydroxyl group, or a methoxy group)

The invention discloses a catalytic asymmetric synthesis method of a beta-substituted gamma-butyrolactone compound, which comprises the following steps: under the action of palladium acetate, a chiral ligand, an acidic additive, an oxidant and a pro-oxidant, reacting an allyl alcohol compound with an alkenyl ether compound in an organic solvent to obtain the beta-substituted gamma-butyrolactone compound, the invention also provides podophyllotoxin, a homologue thereof and a synthesis method of a chiral intermediate compound in a synthesis process of the podophyllotoxin. According to the method, one-pot reaction / oxidation reaction is adopted, so that catalytic asymmetric synthesis of the beta-substituted-gamma-butyrolactone compound is economically and efficiently realized; the reaction substrates are two chemical raw materials and are good in universality, reaction conditions are mild, the process is simple and safe, and operation is easy and convenient.

The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a compound traditional Chinese medicine composition for treating skin warts and condyloma and application thereof. Raw materials of the compound traditional Chinese medicine composition comprise the following components: astilbin, sophoramine, houttuynia cordata, honeycomb, podophyllotoxin, cumin and brucea javanica. A preparation method comprises the following steps: first, extracting brucea javanica oil by pressing brucea javanica to obtain residues; then, adding houttuynia cordata, honeycomb, cumin and the residues into water to obtain decoction by boiling, adding tannase into the decoction to obtain enzyme hydrolysate by enzymolysis, inactivating, and finally, uniformly mixing and dispersing the enzyme hydrolysate, brucea javanica oil, astilbin, sophoramine and podophyllotoxin to obtain the compound traditional Chinese medicine composition. The application has the synergistic effect which is significantly better than that of the original traditional Chinese medicine prescription, and has a good application market prospect for treating skin warts and condyloma.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a compound traditional Chinese medicine composition for treating skin warts and condyloma and application thereof. The compound traditional Chinese medicine composition is prepared from the following raw material components: astilbin, sophocarpidine, herba houttuyniae, honeycomb, podophyllotoxin, fructus cnidii and brucea javanica. The preparation method comprises the following steps: firstly, squeezing the brucea javanica to extract brucea javanica oil to obtain decoction dregs; the preparation method comprises the following steps: adding water into herba houttuyniae, nidus vespae, fructus cnidii and medicine residues, decocting to obtain decoction, adding tannase into the decoction for enzymolysis to obtain enzymatic hydrolysate, inactivating, and finally mixing and dispersing the enzymatic hydrolysate, brucea javanica oil, astilbin, sophocarpidine and podophyllotoxin uniformly to obtain the medicine. All the components are compatible, and the synergistic effect is remarkably superior to that of an original traditional Chinese medicine prescription; the traditional Chinese medicine composition has a good application market prospect for treating skin warts and condyloma.

The invention discloses application of podophyllotoxin in preparation of a marine antifouling agent for preventing adhesion of large fouling organisms. The invention also provides a marine antifouling agent taking podophyllotoxin or the derivative thereof as an effective component. The antifouling agent can optionally contain auxiliary components such as isothiazolinone compounds and the like, and can be prepared into antifouling paint to be applied to the surfaces of marine artificial facilities such as culture netting, ships, wharfs and the like in manners of spraying, dipping and the like. The podophyllotoxin has natural biological activity, an environment-friendly antifouling scheme is provided, the problems that in the existing antifouling technology, an antifouling agent is not high in activity, large in addition amount and poor in environmental friendliness are solved, and the podophyllotoxin is particularly suitable for preventing and treating attachment of large fouling organisms such as perna viridis.

The invention belongs to the technical field of organic matter synthesis, and particularly relates to a preparation method and application of a synthetic intermediate of a podophyllotoxin compound and a method for synthesizing the podophyllotoxin compound. According to the preparation method provided by the invention, 6-allyl heliotropin is taken as an initial raw material, a core tetracyclic skeleton of podophyllotoxin is constructed through an I2-mediated oxidative free radical cyclization lactonization method, and a synthetic intermediate of a podophyllotoxin compound is simply and conveniently synthesized. Furthermore, 11 kinds of podophyllotoxin family molecules can be integrally and asymmetrically synthesized by the synthesized intermediate compound 6 and compound 7 through 1-3 steps of reaction, so that the preparation method provided by the invention can efficiently construct a skeleton structure of podophyllotoxin, avoids the use of transition metals, enriches synthesis strategies of podophyllotoxin natural products, and has a good application prospect. And a novel, green and simple synthetic route is provided for synthesis of corresponding active natural products and similar structures.

The present invention discloses a real-time monitoring system for the extraction of podophyllotoxin based on intelligent sensors, specifically relating to the field of podophyllotoxin extraction, including a data batch division module, a data acquisition module, a data analysis module, a data comprehensive evaluation module, and a real-time feedback warning module. The data acquisition module includes a raw material data acquisition unit and a feedback data acquisition unit, which are used to collect target data in real time and transmit the collected data to the data analysis module; the data analysis module includes a raw material data analysis unit and a feedback data analysis unit, which are used to analyze the data transmitted by the data acquisition module; the data comprehensive evaluation module includes a real-time monitoring data analysis unit for the extraction of podophyllotoxin by intelligent sensors, which is used to comprehensively analyze the data transmitted by the data analysis module; by dividing various types of data in the process of podophyllotoxin extraction into different acquisition batches, the present invention significantly improves the accuracy and timeliness of monitoring.

The utility model provides a kind of sharp condyloma acuminatum traditional chinese medicine external application pollution-preventing patch, it is related to medical technology field, including protective layer, control release layer, drug-loading layer and adhesive layer, the control release layer is below protective layer, the drug-loading layer is below control release layer, the adhesive layer is below drug-loading layer;The drug-loading layer includes grid structure layer, brucea javanica bitter alcohol layer and porous starch microsphere layer, the brucea javanica bitter alcohol layer and porous starch microsphere layer are inside grid structure layer;The utility model adopts multilayer composite structure, treatment main body is drug-loading layer, treat by podophyllotoxin cooperation brucea javanica bitter alcohol layer, brucea javanica bitter alcohol can alleviate symptom and auxiliary remove wart body by the effect of heat-clearing and detoxifying, antiviral and erosion wart, podophyllotoxin is cell division inhibitor, can directly destroy wart body, treatment effect is good, medicine is fixed by grid structure layer, podophyllotoxin is loaded by porous starch microsphere layer, improves the stability of drug loading.

The utility model relates to the technical field of extraction devices, and discloses a microwave extraction device for podophyllotoxin extraction, which comprises a stirring device, the inner cavity of the stirring device is fixedly connected with a stirring inner wall, the side surface of the stirring inner wall is provided with a movable telescopic hole, the side surface of the stirring inner wall is provided with a crushing cutter clamping port, and the crushing cutter clamping port is provided with a crushing cutter. A sealing ring is fixedly connected to the side face of the movable telescopic hole, a telescopic rod is movably connected to the side wall of the inner ring of the movable telescopic hole, a movable fixing block is fixedly connected to the side face of the telescopic rod, an inner wall scraping ring is fixedly connected to the side face of the telescopic rod, and a telescopic pushing air cylinder is fixedly connected to the side face of the movable fixing block. According to the microwave extraction device with a crushing and stirring structure and a good stirring effect, sufficient mixing between a solvent and a material can be ensured, so that the contact area between the solvent and the material is increased, and the solvent can more quickly permeate into the material under the action of microwaves and is in effective contact and reaction with podophyllotoxin molecules.

The invention belongs to the field of basic medicine, and particularly relates to a method for establishing a blood coagulation disorder animal model, which comprises the following steps of: performing intragastric administration on a healthy rat with a preparation containing podophyllotoxin according to the dose of 10mg / kg per day, and continuously performing administration for 4 days; wherein the drug administration can induce the rat to generate blood coagulation disorder phenotypes, and the phenotypes at least comprise prothrombin time prolonging, activated partial thromboplastin time prolonging and thrombin time prolonging. According to the establishment method of the blood coagulation disorder animal model, it is determined that podophyllotoxin can cause whole-body blood coagulation disorder, and the stable and repeatable blood coagulation disorder animal model is provided.

The invention belongs to the field of basic medicine, and particularly relates to a podophyllotoxin-based zebrafish intestinal brain axis toxicity modeling method which comprises the following steps: step 1, exposure treatment of zebrafish: selecting wild type AB-line zebrafish which is fertilized for 3 days, and treating for 2 days by dissolving podophyllotoxin in water; step 2, multi-omics joint detection: (1) extracting zebra fish intestinal tract and brain contents, performing 16S rRNA sequencing, quantitative metabolite detection and transcriptomics detection, and identifying substances; and 3, performing correlation analysis on the toxicity of the intestinal brain axis: performing correlation analysis on the differential flora, the differential metabolite and the differential expression gene identified in the step 2, and constructing an intestinal flora-metabolite-brain gene regulatory network. The invention aims to solve the problems that podophyllotoxin toxicity research in the prior art is limited to single organ evaluation, and an internal association mechanism among intestinal injury, dysbacteriosis, metabolic disorder and brain toxicity induced by podophyllotoxin is not revealed from an intestinal brain axis level system.

The invention discloses an artificial intelligence-based podophyllotoxin purification production optimization management system, and particularly relates to the technical field of podophyllotoxin purification production management. A high-speed camera is combined with a deep learning model to carry out visual sorting on raw materials to remove waste materials in the raw materials, and unqualified raw materials detected by near infrared spectroscopy are subjected to trigger pretreatment; the waste removal accuracy, the pretreatment triggering accuracy and the sorting precision are improved; dynamic counter-current extraction is adopted, so that the extraction efficiency and the purity of podophyllotoxin in a light phase are improved; the gradient slope or flow velocity is adjusted based on the real-time UV absorption peak shape AI, the leading edge and trailing of an elution peak are identified through the AI, only the middle section of the main peak is collected, the low-purity edge part is abandoned, when the main peak of podophyllotoxin is detected, a fraction collector is triggered to be started to collect high-purity eluent, and when impurity peaks are detected, the fraction collector is automatically switched to a waste liquid pipeline, so that the recovery rate of target components is increased; the purity and the yield of the podophyllotoxin are favorably improved.

The application discloses a kind of rapid proliferation methods of arisaema consanguineum callus, belong to plant tissue culture technical field, break arisaema consanguineum seed dormancy by sand storage method, seed is soaked after distilled water and gibberellin solution and river sand is fully mixed, after storage germination is sowed in the soil added with equal amount of matrix, arisaema consanguineum seedling is obtained after cultivation;Take arisaema consanguineum seedling fresh leaf as explant, cut into small pieces after disinfection on the MS medium added with hormone and cultivate 30d to induce callus, the callus induced is cultivated to proliferate at the optimum temperature of 15 DEG C, inoculate to the same fresh medium after cultivation 20d to prevent browning generation, to obtain high-quality callus, after every 20d, fresh medium can be replaced to realize arisaema consanguineum callus rapid mass proliferation.By the above method, a large amount of high-quality arisaema consanguineum callus can be quickly obtained, which provides sufficient materials for podophyllotoxin extraction and medicinal value development.

The present application relates to the field of pharmaceutical chemistry technology, and specifically discloses a podophyllotoxin derivative, its preparation method, and application. The general structural formula of the podophyllotoxin derivative is: wherein the general structural formula of R is: R1 is selected from one of CH3, (CH2)4CH3, citronellol, and piperonyl alcohol; X is selected from one of S, S-S, Se, O, C, and C-C; and n is 1 or 2. The podophyllotoxin derivatives in this application have good in vitro antitumor activity, specifically releasing or accumulating drugs in the tumor environment to achieve low-toxicity, high-efficiency, targeted tumor treatment.

The application discloses an indazole-nitrogen-substituted podophyllotoxin derivative, a synthesis method and application thereof, and belongs to the field of medicine. Different substituents, such as methyl, ethyl, propyl, benzyl, fluorine atom, chlorine atom, bromine atom, iodine atom and the like, are introduced into 1 and 3 positions of indazole podophyllotoxin to obtain a podophyllotoxin derivative shown in formula (V) with significantly improved antitumor activity. In vitro tumor cell activity inhibition experiments show that the antitumor activity of the compound shown in formula (V) is significantly improved compared with that of podophyllotoxin.

The invention relates to the technical field of biological medicines, in particular to application of podophyllotoxin drugs in preparation of products for inducing endoplasmic reticulum autophagy. According to the invention, it is found for the first time that podophyllotoxin drugs up-regulate TEX264 through OTUD3 to induce endoplasmic reticulum autophagy. The important target of the podophyllotoxin medicine is further clarified while a new action mechanism of the podophyllotoxin medicine is found, and a new theoretical basis is provided for clinical application of the podophyllotoxin medicine.

The present invention belongs to the technical field of cosmetics, and particularly relates to a whitening composition containing American ginseng and mulberry leaves and its uses. The whitening composition comprises the following components: mulberry leaves, American ginseng, (-)-Podophyllotoxin, and (+)-Glaucarubinone. Experimental results show that the composition of the present invention has obvious inhibitory effects on both tyrosinase activity and the expression of tyrosine-related proteins, thereby achieving the effect of skin whitening. Therefore, the whitening composition of the present invention can be used to prepare various skin whitening products, or products for inhibiting melanin synthesis, or products for improving skin pigmentation, and has broad market prospects.

The utility model discloses a filter pressing structure for preparing podophyllotoxin, which belongs to the technical field of podophyllotoxin preparation, and comprises a frame body mechanism and a plurality of filter pressing plate mechanisms, the frame body mechanism is provided with a plurality of filter pressing plate mechanisms for solid-liquid separation in a sliding manner, and the filter pressing plate mechanisms are arranged on the frame body mechanism in a sliding manner. A plug pin mechanism for preventing the filter pressing plate mechanism from shaking is inserted into the filter pressing plate mechanism, and a screwing mechanism is in threaded connection with the plug pin mechanism. Through the arrangement of the frame body mechanism, the filter pressing plate mechanism, the bolt mechanism and the screwing mechanism, a worker can conveniently and quickly replace the filter pressing plates, so that the worker can conveniently maintain and replace the filter pressing plates, and the worker can conveniently increase and reduce the number of the filter pressing plates; through the arrangement of the square groove, the transverse through groove, the longitudinal through groove and the liquid discharge channel, the volume of the inner cavity of the filter pressing plate and the flow rate of liquid are effectively improved, and the working efficiency of the device is favorably improved.