11 results about "Podophyllotoxin" patented technology

The application belongs to the technical field of animal models for basic scientific research, and particularly relates to a method for establishing an acute lung injury animal model, which comprises the following steps: sending a podophyllotoxin or a podophyllotoxin reagent into a rat body through oral administration or intragastric perfusion to obtain the acute lung injury animal model. The method for establishing the acute lung injury animal model provided by the application uses the podophyllotoxin as an inducer for establishing a mouse acute lung injury model, and the mouse can orally take or intragastrically perfuse the podophyllotoxin to achieve the method, without needing a special medicine injection device, so that the method is simple in medicine taking, few in experimental steps, and does not need complex surgical operations.

A whitening composition containing the following components: Mori folium, Panacis quinquefolii radix, (-)-podophyllotoxin, and (+)-glaucarubinone. Further provided is the use of the whitening composition in the preparation of a product for whitening the skin, or a product for inhibiting melanin synthesis, or a product for improving skin pigmentation.

The application discloses a class of podophyllotoxin triazole ether derivatives, a preparation method and application thereof, belongs to the technical field of chemical pharmacy, and particularly relates to a class of podophyllotoxin triazole derivatives and application thereof in preparation of antitumor drugs. The application combines a triazole structure with a podophyllotoxin parent body by using a click chemistry reaction, designs and synthesizes a series of novel 1,2,3-triazole podophyllotoxin derivatives, optimizes the kinetic characteristics of the podophyllotoxin by introducing the triazole structure, and improves the anticancer effect and drug property. Molecular docking shows that the series of compounds have strong binding energy on AKT1 and tubulin, can target inhibit tubulin / AKT1, and in-vitro antitumor activity research shows that the molecules have strong inhibitory activity on the proliferation of human colon cancer cell strains, and can provide a new strategy for structural derivatization of podophyllotoxin.

The invention belongs to the field of basic medical research, and particularly relates to a testicular toxicity animal model establishing method which comprises the following steps: performing intragastric administration on male rats with a preparation containing podophyllotoxin according to the dosage of 15 mg / kg per day; the medicine is continuously administrated for 4 days; according to the method, the rat can be induced to generate testicular toxicity phenotypes, and the phenotypes at least include that the number of sperms is remarkably reduced, the activity of the sperms is reduced, the testosterone level of serum is abnormally increased, and spermatogenic tubules in testicular tissue are irregular in shape and spermatogenic epithelium is thinned.

The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a compound traditional Chinese medicine composition for treating skin warts and condyloma and application thereof. Raw materials of the compound traditional Chinese medicine composition comprise the following components: astilbin, sophoramine, houttuynia cordata, honeycomb, podophyllotoxin, cumin and brucea javanica. A preparation method comprises the following steps: first, extracting brucea javanica oil by pressing brucea javanica to obtain residues; then, adding houttuynia cordata, honeycomb, cumin and the residues into water to obtain decoction by boiling, adding tannase into the decoction to obtain enzyme hydrolysate by enzymolysis, inactivating, and finally, uniformly mixing and dispersing the enzyme hydrolysate, brucea javanica oil, astilbin, sophoramine and podophyllotoxin to obtain the compound traditional Chinese medicine composition. The application has the synergistic effect which is significantly better than that of the original traditional Chinese medicine prescription, and has a good application market prospect for treating skin warts and condyloma.

The invention discloses application of podophyllotoxin in preparation of a marine antifouling agent for preventing adhesion of large fouling organisms. The invention also provides a marine antifouling agent taking podophyllotoxin or the derivative thereof as an effective component. The antifouling agent can optionally contain auxiliary components such as isothiazolinone compounds and the like, and can be prepared into antifouling paint to be applied to the surfaces of marine artificial facilities such as culture netting, ships, wharfs and the like in manners of spraying, dipping and the like. The podophyllotoxin has natural biological activity, an environment-friendly antifouling scheme is provided, the problems that in the existing antifouling technology, an antifouling agent is not high in activity, large in addition amount and poor in environmental friendliness are solved, and the podophyllotoxin is particularly suitable for preventing and treating attachment of large fouling organisms such as perna viridis.

The utility model provides a kind of sharp condyloma acuminatum traditional chinese medicine external application pollution-preventing patch, it is related to medical technology field, including protective layer, control release layer, drug-loading layer and adhesive layer, the control release layer is below protective layer, the drug-loading layer is below control release layer, the adhesive layer is below drug-loading layer;The drug-loading layer includes grid structure layer, brucea javanica bitter alcohol layer and porous starch microsphere layer, the brucea javanica bitter alcohol layer and porous starch microsphere layer are inside grid structure layer;The utility model adopts multilayer composite structure, treatment main body is drug-loading layer, treat by podophyllotoxin cooperation brucea javanica bitter alcohol layer, brucea javanica bitter alcohol can alleviate symptom and auxiliary remove wart body by the effect of heat-clearing and detoxifying, antiviral and erosion wart, podophyllotoxin is cell division inhibitor, can directly destroy wart body, treatment effect is good, medicine is fixed by grid structure layer, podophyllotoxin is loaded by porous starch microsphere layer, improves the stability of drug loading.

The invention belongs to the field of basic medicine, and particularly relates to a method for establishing a blood coagulation disorder animal model, which comprises the following steps of: performing intragastric administration on a healthy rat with a preparation containing podophyllotoxin according to the dose of 10mg / kg per day, and continuously performing administration for 4 days; wherein the drug administration can induce the rat to generate blood coagulation disorder phenotypes, and the phenotypes at least comprise prothrombin time prolonging, activated partial thromboplastin time prolonging and thrombin time prolonging. According to the establishment method of the blood coagulation disorder animal model, it is determined that podophyllotoxin can cause whole-body blood coagulation disorder, and the stable and repeatable blood coagulation disorder animal model is provided.

The invention belongs to the field of basic medicine, and particularly relates to a podophyllotoxin-based zebrafish intestinal brain axis toxicity modeling method which comprises the following steps: step 1, exposure treatment of zebrafish: selecting wild type AB-line zebrafish which is fertilized for 3 days, and treating for 2 days by dissolving podophyllotoxin in water; step 2, multi-omics joint detection: (1) extracting zebra fish intestinal tract and brain contents, performing 16S rRNA sequencing, quantitative metabolite detection and transcriptomics detection, and identifying substances; and 3, performing correlation analysis on the toxicity of the intestinal brain axis: performing correlation analysis on the differential flora, the differential metabolite and the differential expression gene identified in the step 2, and constructing an intestinal flora-metabolite-brain gene regulatory network. The invention aims to solve the problems that podophyllotoxin toxicity research in the prior art is limited to single organ evaluation, and an internal association mechanism among intestinal injury, dysbacteriosis, metabolic disorder and brain toxicity induced by podophyllotoxin is not revealed from an intestinal brain axis level system.

The application discloses an indazole-nitrogen-substituted podophyllotoxin derivative, a synthesis method and application thereof, and belongs to the field of medicine. Different substituents, such as methyl, ethyl, propyl, benzyl, fluorine atom, chlorine atom, bromine atom, iodine atom and the like, are introduced into 1 and 3 positions of indazole podophyllotoxin to obtain a podophyllotoxin derivative shown in formula (V) with significantly improved antitumor activity. In vitro tumor cell activity inhibition experiments show that the antitumor activity of the compound shown in formula (V) is significantly improved compared with that of podophyllotoxin.

The utility model discloses a filter pressing structure for preparing podophyllotoxin, which belongs to the technical field of podophyllotoxin preparation, and comprises a frame body mechanism and a plurality of filter pressing plate mechanisms, the frame body mechanism is provided with a plurality of filter pressing plate mechanisms for solid-liquid separation in a sliding manner, and the filter pressing plate mechanisms are arranged on the frame body mechanism in a sliding manner. A plug pin mechanism for preventing the filter pressing plate mechanism from shaking is inserted into the filter pressing plate mechanism, and a screwing mechanism is in threaded connection with the plug pin mechanism. Through the arrangement of the frame body mechanism, the filter pressing plate mechanism, the bolt mechanism and the screwing mechanism, a worker can conveniently and quickly replace the filter pressing plates, so that the worker can conveniently maintain and replace the filter pressing plates, and the worker can conveniently increase and reduce the number of the filter pressing plates; through the arrangement of the square groove, the transverse through groove, the longitudinal through groove and the liquid discharge channel, the volume of the inner cavity of the filter pressing plate and the flow rate of liquid are effectively improved, and the working efficiency of the device is favorably improved.