7 results about "Astilbin" patented technology

The invention provides a pharmaceutical composition for treating prostatitis and a preparation method thereof. The pharmaceutical composition is prepared from the following raw materials in parts by weight: 20 to 60 parts of palmatine, 20 to 60 parts of paeoniflorin, 10 to 30 parts of formononetin, 20 to 60 parts of astilbin and 10 to 40 parts of stigmasterol. In-vitro cell tests and in-vivo animal experiments prove that the pharmaceutical composition has the effect of relieving the chronic prostatitis reaction.

The present application belongs to the field of biochemistry and health food, and particularly relates to a method for extracting astilbin by using a natural eutectic solvent and application. The present application uses a novel environment-friendly solvent, a natural eutectic solvent (choline chloride-lactic acid), to extract astilbin, and the extraction rate of astilbin is twice as high as that of ethanol extraction. The mechanism of extracting astilbin by using the natural eutectic solvent is obtained by using a molecular dynamics simulation technology. The natural eutectic solvent extract of astilbin is obtained by using a simulated digestion system, and the bioavailability of astilbin in the natural eutectic solvent extract is 84.1% higher than that of ethanol extract, and the antioxidant capacity is 5 times higher than that of ethanol extract. The protection of the natural eutectic solvent in the simulated digestion solves the problem of low bioavailability of active ingredients in each stage of digestion.

The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a compound traditional Chinese medicine composition for treating skin warts and condyloma and application thereof. Raw materials of the compound traditional Chinese medicine composition comprise the following components: astilbin, sophoramine, houttuynia cordata, honeycomb, podophyllotoxin, cumin and brucea javanica. A preparation method comprises the following steps: first, extracting brucea javanica oil by pressing brucea javanica to obtain residues; then, adding houttuynia cordata, honeycomb, cumin and the residues into water to obtain decoction by boiling, adding tannase into the decoction to obtain enzyme hydrolysate by enzymolysis, inactivating, and finally, uniformly mixing and dispersing the enzyme hydrolysate, brucea javanica oil, astilbin, sophoramine and podophyllotoxin to obtain the compound traditional Chinese medicine composition. The application has the synergistic effect which is significantly better than that of the original traditional Chinese medicine prescription, and has a good application market prospect for treating skin warts and condyloma.

The application discloses a quality detection method of a Yishen Qingli Huoxue recipe, which comprises preparation of a test sample solution, preparation of a standard solution, preparation of a standard curve and content determination. The application screens an optimal test sample preparation method, a preparation method of a control sample and optimal chromatographic conditions and mass spectrometric analysis conditions through a large number of experiments, and establishes a content determination method of the Yishen Qingli Huoxue recipe, which can simultaneously determine the contents of 11 compounds with different structural types, such as resveratrol, rhein, formononetin, aloe-emodin, emodin, chlorogenic acid, polydatin, astilbin, hyperoside, calycosin-7-glucoside and beta-ecdysterone. The method is favorable for comprehensively monitoring the quality of the Yishen Qingli Huoxue recipe and guaranteeing clinical curative effects. Moreover, the method has the advantages of good stability, high accuracy, good repeatability and high precision.

The application discloses a traditional Chinese medicine combined medicine for treating gout of animals and application thereof, and belongs to the technical field of animal medicine. The core active components of the traditional Chinese medicine combined medicine are composed of astilbin, tanshinone II A and tetrahydrocurcumin, wherein the preferred mass ratio of the components is 4:1:3. Pharmacodynamic researches show that the composition has a significant synergistic effect, can effectively reduce the blood uric acid level of hyperuricemia animals, and synergistically inhibit xanthine oxidase (XOD) activity. Compared with existing chemical medicines, the application reduces the toxic side effects and drug resistance possibility while ensuring efficient reduction of uric acid concentration through synergistic combination of the medicines, provides a safe, efficient and economical new scheme for treating gout of animals, and has important application value.

This invention discloses a method for constructing a fingerprint spectrum of a traditional Chinese medicine composition containing Danshen and Astragalus. The method includes the following steps: taking a test solution and a reference solution of the traditional Chinese medicine composition for detection; obtaining the content information of the reference standard of the traditional Chinese medicine composition based on the detection results; wherein the traditional Chinese medicine composition includes Danshen, Polygonatum odoratum, Akebia trifoliata, Ligusticum striatum, Glycyrrhiza uralensis, Atractylodes macrocephala, Smilax glabra, Saposhnikovia divaricata, Rehmannia glutinosa, Ophiopogon japonicus, and Astragalus membranaceus; and the reference standard is caffeic acid, glycyrrhizin, rehmannia glutinosa D, cimicifugain, astilbin, salvianolic acid B, and ammonium glycyrrhizate. The detection method of this invention is simple, accurate, efficient, stable, precise, reproducible, convenient, and easy to master. It can effectively save energy and reduce analytical costs, while this technology is not disclosed in the prior art.

The invention discloses an alpha-L-rhamnosidase mutant capable of efficiently converting astilbin and application of the alpha-L-rhamnosidase mutant, and belongs to the technical field of gene engineering and enzyme engineering. The amino acid sequence of the alpha-L-rhamnosidase mutant is shown as SEQ ID NO.13. The relative enzyme activity of the mutant is 14.08 times that of a wild type, the enzyme activity in high-density fermentation can reach 1800 U / mL, the conversion rate of 95% or above can be achieved within 48-72 hours within the wide substrate concentration range of 5-50 g / L, and the production efficiency of dihydroquercetin is remarkably improved. The invention provides an efficient and green biocatalyst for preparing dihydroquercetin by an enzyme method, overcomes the limitations of the existing extraction method and chemical synthesis method in the aspects of efficiency, environmental protection and cost, and has a good industrial application prospect.