19 results about "Astilbin" patented technology

The invention discloses a fingerprint spectrum construction method of a traditional Chinese medicine composition containing salvia miltiorrhiza and astragalus membranaceus. The construction method comprises the following steps: taking a test solution and a reference solution of the traditional Chinese medicine composition for detection; obtaining content information of a reference substance of the traditional Chinese medicine composition according to a detection result; wherein the traditional Chinese medicine composition comprises salvia miltiorrhiza, radix polygonati officinalis, caulis clematidis armandii, ligusticum, liquorice, bighead atractylodes rhizome, rhizoma smilacis glabrae, radix saposhnikoviae, radix rehmanniae recen, radix ophiopogonis and astragalus membranaceus, and the reference substances are caffeic acid, liquiritin, rehmannia glutinosa libosch D, cimicifugin, astilbin, salvianolic acid B and ammonium glycyrrhizinate. The detection method is simple, accurate, efficient, good in stability, high in precision, good in reproducibility, convenient and easy to master, energy can be effectively saved, the analysis cost is reduced, and the technical content is not disclosed in the prior art.

The invention provides a pharmaceutical composition for treating prostatitis and a preparation method thereof. The pharmaceutical composition is prepared from the following raw materials in parts by weight: 20 to 60 parts of palmatine, 20 to 60 parts of paeoniflorin, 10 to 30 parts of formononetin, 20 to 60 parts of astilbin and 10 to 40 parts of stigmasterol. In-vitro cell tests and in-vivo animal experiments prove that the pharmaceutical composition has the effect of relieving the chronic prostatitis reaction.

The invention belongs to the field of medicines, and particularly relates to a quality detection method of a clearing and deacidification mixture. According to the invention, a characteristic spectrum method for simultaneously detecting 10 main active components (paeoniflorin, puerarin, gentiopicroside, loganic acid, astilbin, benzoylpaeoniflorin, resveratrol, rhein, rhizoma dioscoreae septemlobae A and rhizoma dioscoreae septemlobae B) in the mixture is established for the first time, the limitation of single component quality control is overcome, and the overall quality of a medicine can be evaluated more comprehensively and objectively; by optimizing a sample pretreatment process and chromatographic gradient elution conditions, interference of a complex matrix is effectively eliminated, and good separation of each component is ensured. The method is high in specificity, negative, free of interference and capable of accurately recognizing target components.

The present application belongs to the field of biochemistry and health food, and particularly relates to a method for extracting astilbin by using a natural eutectic solvent and application. The present application uses a novel environment-friendly solvent, a natural eutectic solvent (choline chloride-lactic acid), to extract astilbin, and the extraction rate of astilbin is twice as high as that of ethanol extraction. The mechanism of extracting astilbin by using the natural eutectic solvent is obtained by using a molecular dynamics simulation technology. The natural eutectic solvent extract of astilbin is obtained by using a simulated digestion system, and the bioavailability of astilbin in the natural eutectic solvent extract is 84.1% higher than that of ethanol extract, and the antioxidant capacity is 5 times higher than that of ethanol extract. The protection of the natural eutectic solvent in the simulated digestion solves the problem of low bioavailability of active ingredients in each stage of digestion.

The present disclosure discloses a method for constructing a chicory poria complex fingerprint, including (1) preparing a sample solution of a chicory poria complex, (2) preparing a mixed control solution using chlorogenic acid, 6'-O-β-D-glucosylgentiopicroside, swerthiamarin, gentiopicroside, swelloside, 3-O-caffeoylshikimic acid, neoastilbin, astilbin, rutin, isoastilbin, isochlorogenic acid C, quercitrin, kaempferol-3-O-rutinoside, and narcissine, and (3) injecting the sample solution and the mixed control solution into a high-performance liquid chromatograph, respectively, and performing a similarity analysis on the resulting chromatograms to obtain a chicory poria complex fingerprint. The present disclosure also provides a chicory poria complex fingerprint constructed by the construction method and its application to identifying and / or evaluating the quality of chicory poria complexes. The disclosed chicory poria compound fingerprint can comprehensively, objectively, and accurately detect and evaluate the quality of chicory poria compound, thereby ensuring safe and effective medication for patients.

The invention relates to the technical field of pharmacy, and provides a preparation process of tendon-relaxing and detoxifying granules, which comprises the following steps: step 1, decocting medicines, and adding water for decocting; step 2, adding conventional decoction medicines, decocting, and then filtering to obtain filtrate for later use; step 3, taking medicine residues in the step 2, adding water, decocting, adding uncaria, decocting, and filtering to obtain filtrate for later use; step 4, mixing the medicine liquid in the step 2 and the medicine liquid in the step 3, concentrating under reduced pressure to obtain thick paste, drying under reduced pressure, crushing, and sieving with a 60-mesh sieve to obtain medicine powder; and 5, taking other auxiliary materials, uniformly mixing, adding the medicinal powder in the step 4, uniformly mixing, and granulating. By optimizing the extraction efficiency, the gastrodin content reaches 34.717 mg / g, the astilbin extraction rate reaches 5.944 mg / g, the particle performance is greatly improved, the forming rate is improved to 93.97% from 83.95%, the particle dissolution time is shorter than 5 minutes, and the requirements of pharmacopeia are met. The 80% ethanol wetting agent reduces the moisture absorption rate to 9.60% and improves the storage stability. And multi-component collaborative optimization is realized through an AHP-EWM weight method.

The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a compound traditional Chinese medicine composition for treating skin warts and condyloma and application thereof. Raw materials of the compound traditional Chinese medicine composition comprise the following components: astilbin, sophoramine, houttuynia cordata, honeycomb, podophyllotoxin, cumin and brucea javanica. A preparation method comprises the following steps: first, extracting brucea javanica oil by pressing brucea javanica to obtain residues; then, adding houttuynia cordata, honeycomb, cumin and the residues into water to obtain decoction by boiling, adding tannase into the decoction to obtain enzyme hydrolysate by enzymolysis, inactivating, and finally, uniformly mixing and dispersing the enzyme hydrolysate, brucea javanica oil, astilbin, sophoramine and podophyllotoxin to obtain the compound traditional Chinese medicine composition. The application has the synergistic effect which is significantly better than that of the original traditional Chinese medicine prescription, and has a good application market prospect for treating skin warts and condyloma.

The application discloses a quality detection method of a Yishen Qingli Huoxue recipe, which comprises preparation of a test sample solution, preparation of a standard solution, preparation of a standard curve and content determination. The application screens an optimal test sample preparation method, a preparation method of a control sample and optimal chromatographic conditions and mass spectrometric analysis conditions through a large number of experiments, and establishes a content determination method of the Yishen Qingli Huoxue recipe, which can simultaneously determine the contents of 11 compounds with different structural types, such as resveratrol, rhein, formononetin, aloe-emodin, emodin, chlorogenic acid, polydatin, astilbin, hyperoside, calycosin-7-glucoside and beta-ecdysterone. The method is favorable for comprehensively monitoring the quality of the Yishen Qingli Huoxue recipe and guaranteeing clinical curative effects. Moreover, the method has the advantages of good stability, high accuracy, good repeatability and high precision.

The application discloses a kind of construction methods of Jujing compound fingerprint, comprising: (1) Jujing compound is made into test solution;(2) using chlorogenic acid, 6'-O-β-D-glucosyl gentiopicroside, swertiamarin, gentiopicroside, swertisin, 3-O-coffeicoyl shikimic acid, astilbin, astilbinin, rutin, isofraxin, isochlorogenic acid C, quercitrin, kaempferol-3-O-rutinoside and zephyranthine preparation mixed reference solution;(3) respectively test solution and mixed reference solution are injected into high performance liquid chromatograph, the similarity analysis is carried out to the obtained chromatogram, and Jujing compound fingerprint is obtained.The application also provides Jujing compound fingerprint obtained by the construction method and its application in identifying and / or evaluating the quality of Jujing compound.The Jujing compound fingerprint of the application can comprehensively, objectively and accurately detect and evaluate the quality of Jujing compound, so as to ensure the safety and effectiveness of patient medication.

The invention discloses a traditional Chinese medicine composition for treating animal gout and application of the traditional Chinese medicine composition, and belongs to the technical field of animal medicine. The core active components of the traditional Chinese medicine composition comprise astilbin, tanshinone IIA and tetrahydrocurcumin, wherein the preferable mass ratio of all the components is 4: 1: 3. Pharmacodynamic studies show that the composition has a significant synergistic effect, can effectively reduce the blood uric acid level of animals with hyperuricemia, and synergistically inhibits the activity of xanthine oxidase (XOD). Compared with existing chemical drugs, the pharmaceutical composition disclosed by the invention has the advantages that through synergistic combination of the drugs, the toxic and side effects and the possibility of drug resistance are effectively reduced while the concentration of uric acid is efficiently reduced, a safe, efficient and economic brand-new scheme is provided for treating animal gout, and the pharmaceutical composition has important application value.

The present application relates to the technical field of kidney disease treatment, and specifically provides application of astilbin in stimulating mesenchymal stem cells in preparation of a medicine for treating diabetic nephropathy. The present application provides application of mesenchymal stem cells in preparation of a medicine for treating kidney disease, wherein 5-20 μg / ml of astilbin is added to cell culture solution during subculture of the mesenchymal stem cells. The present application first discovers that the traditional Chinese medicine monomer astilbin can be used to pretreat bone marrow mesenchymal stem cells with differentiation capacity and potential for treating diabetes, greatly enhancing the treatment effect of bone marrow mesenchymal stem cells on diabetic nephropathy. Animal models also confirm that the astilbin can significantly reduce the urine protein level of a mouse model of diabetic nephropathy, improve kidney function, and significantly reduce the histopathological score of kidney tissue, thereby significantly reducing kidney damage and having a significant therapeutic effect.

The invention belongs to the technical field of traditional Chinese medicines, and provides a pharmaceutical composition for cervical and vaginal lesions as well as a preparation method and application of the pharmaceutical composition. The pharmaceutical composition is prepared from the following components in parts by weight: 0.01 to 1 part of astilbin, 7.5 to 30 parts of radix sophorae flavescentis, 10 to 35 parts of herba houttuyniae, 7.5 to 30 parts of honeycomb, 6 to 24 parts of dysosma versipellis, 8 to 24 parts of fructus cnidii and 2.5 to 5 parts of oleic acid. The pharmaceutical composition disclosed by the invention has a prominent treatment effect on vagina or cervical lesions, especially vaginitis and vagina and cervical squamous intraepithelial neoplasia caused by HPV (Human Papilloma Virus).

The application discloses a traditional Chinese medicine combined medicine for treating gout of animals and application thereof, and belongs to the technical field of animal medicine. The core active components of the traditional Chinese medicine combined medicine are composed of astilbin, tanshinone II A and tetrahydrocurcumin, wherein the preferred mass ratio of the components is 4:1:3. Pharmacodynamic researches show that the composition has a significant synergistic effect, can effectively reduce the blood uric acid level of hyperuricemia animals, and synergistically inhibit xanthine oxidase (XOD) activity. Compared with existing chemical medicines, the application reduces the toxic side effects and drug resistance possibility while ensuring efficient reduction of uric acid concentration through synergistic combination of the medicines, provides a safe, efficient and economical new scheme for treating gout of animals, and has important application value.

The invention discloses application of astilbin in preparation of a medicine for treating myocardial infarction, and belongs to the technical field of biological medicines. Experiments prove that astilbin can remarkably inhibit ventricular pathological remodeling after MI mouse infarction and improve the heart function, and the effect of astilbin is closely related to regulation and control of EGFR and other core targets, intervention of PI3K-AKT signal channels, further inhibition of inflammatory response, reduction of oxidative stress, reduction of myocardial cell apoptosis and inhibition of myocardial fibrosis. The invention preliminarily clarifies the action mechanism and material basis of AST for inhibiting post-infarction ventricular pathological remodeling, and provides theoretical support for clinical application of AST.

The application discloses a use of astilbin in preparation of a medicine for improving secondary brain injury after cerebral hemorrhage. The application is proved by a behavior experiment, a Perls staining experiment, a Nissl staining experiment, a Tunel staining experiment, an Evans blue penetration experiment, an ELISA experiment and an immunofluorescence staining experiment. The experiment proves that astilbin can improve the prognosis of secondary brain injury such as edema, iron deposition and neuron necrosis and apoptosis after cerebral hemorrhage by promoting Treg cells to be enriched in the brain tissue around the brain hematoma, secreting anti-inflammatory factors and creating an environment for inhibiting inflammation, and reduce the nerve function injury caused by cerebral hemorrhage. The application provides an experimental basis and a theoretical basis for astilbin to treat secondary brain injury after cerebral hemorrhage.

This invention discloses a method for constructing a fingerprint spectrum of a traditional Chinese medicine composition containing Danshen and Astragalus. The method includes the following steps: taking a test solution and a reference solution of the traditional Chinese medicine composition for detection; obtaining the content information of the reference standard of the traditional Chinese medicine composition based on the detection results; wherein the traditional Chinese medicine composition includes Danshen, Polygonatum odoratum, Akebia trifoliata, Ligusticum striatum, Glycyrrhiza uralensis, Atractylodes macrocephala, Smilax glabra, Saposhnikovia divaricata, Rehmannia glutinosa, Ophiopogon japonicus, and Astragalus membranaceus; and the reference standard is caffeic acid, glycyrrhizin, rehmannia glutinosa D, cimicifugain, astilbin, salvianolic acid B, and ammonium glycyrrhizate. The detection method of this invention is simple, accurate, efficient, stable, precise, reproducible, convenient, and easy to master. It can effectively save energy and reduce analytical costs, while this technology is not disclosed in the prior art.

The invention discloses an alpha-L-rhamnosidase mutant capable of efficiently converting astilbin and application of the alpha-L-rhamnosidase mutant, and belongs to the technical field of gene engineering and enzyme engineering. The amino acid sequence of the alpha-L-rhamnosidase mutant is shown as SEQ ID NO.13. The relative enzyme activity of the mutant is 14.08 times that of a wild type, the enzyme activity in high-density fermentation can reach 1800 U / mL, the conversion rate of 95% or above can be achieved within 48-72 hours within the wide substrate concentration range of 5-50 g / L, and the production efficiency of dihydroquercetin is remarkably improved. The invention provides an efficient and green biocatalyst for preparing dihydroquercetin by an enzyme method, overcomes the limitations of the existing extraction method and chemical synthesis method in the aspects of efficiency, environmental protection and cost, and has a good industrial application prospect.

The invention discloses a preparation method of a detoxification and leucorrhea arresting mixture, the mixture prepared by the method, and a quality control method and application of the mixture. The mixture is characterized in that in every 1mL of the mixture, the content of psoralen (C11H6O3) is not less than 14.89 [mu] g, and the content of astilbin (C21H22O11) is not less than 0.54 mg. The preparation method comprises the integrated steps of extracting medicinal materials, concentrating an extracting solution until the relative density is 1.06-1.10 at the temperature of 60 DEG C, standing and purifying, and adding stevioside and sodium benzoate in a specific proportion to prepare liquid and form. The invention also establishes a comprehensive quality control system covering thin-layer chromatography identification of multiple medicines including ovateleaf holly bark, pericarpium citri reticulatae, liquorice and plantain herb and content determination of the two-index components. In-vitro and clinical evidences show that the mixture has antibacterial and anti-inflammatory effects and has a good application prospect in treating gynecological diseases related to human papilloma virus (HPV) infection.

The present application belongs to the field of medicine, and particularly relates to a quality detection method of a Shengqing Jiangsuan mixture. The present application firstly establishes a characteristic spectrum method for simultaneously detecting 10 main active components (paeoniflorin, puerarin, gentiopicroside, loganin acid, astilbin, benzoylpaeoniflorin, resveratrol, rhein, pachymaran A and pachymaran B) in the mixture, overcomes the limitation of single component control quality, and can more comprehensively and objectively evaluate the overall quality of the medicine. Through optimizing the sample pretreatment process and the chromatographic gradient elution conditions, the present application effectively eliminates the interference of the complex matrix, and ensures the good separation of each component. The method has strong specificity, no interference of negative, and can accurately identify the target component.