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Methods for extracting astilbin and dihydroquercetin from engelhardia roxburghiana wall leaves and application of extractives thereof

A technology of dihydroquercetin and astilbin, which is applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of low product yield and complicated operation steps, and achieve high extraction rate and content. , Simple operation, low cost effect

Inactive Publication Date: 2011-11-09
广州医学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the methods of extracting astilbin from the leaves of Huangqi are different, they all use adsorption resin, polyamide column or silica gel column chromatography, and the operation steps are relatively cumbersome.
Dihydroquercetin is usually extracted from larch. It is extracted by polyamide column or liquid-liquid extraction method, and then purified by recrystallization. Usually, decolorization treatment is required, and the yield of the obtained product is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: Extraction method of astilbin and dihydroquercetin

[0021] 1. Extraction: Grind the dried chrysanthemum leaves into coarse powder, add 6 times the amount of 95% ethanol aqueous solution for reflux extraction for the first time, reflux extraction for 2 hours after boiling, and collect the extract; add 5 times the amount of 60% ethanol for the second time The aqueous solution was boiled and then refluxed for 4 hours, and the extract was collected; the two extracts were combined, concentrated under reduced pressure at 60°C until there was no alcohol smell, the volume of the concentrated solution was 0.8 times the volume of the medicinal material, and the precipitate was removed by centrifugation;

[0022] 2. Degreasing: Extract the centrifuged medicinal solution twice with 1.2 times the amount of petroleum ether;

[0023] 3. Extraction: extract the degreased mother liquor with 1.5 times the amount of ethyl acetate for 4 times, combine the ethyl acetate layer...

Embodiment 2

[0026] Embodiment 2: Extraction method of astilbin and dihydroquercetin

[0027] 1. Extraction: Grind the dried chrysanthemum leaves into coarse powder, add 8 times the amount of 95% ethanol aqueous solution for the first time for reflux extraction, reflux extraction for 3 hours after boiling, and collect the extract; add 6 times the amount of 60% ethanol for the second time Aqueous solution, reflux after boiling for 3 hours, collect the extract; add 5 times the amount of 60% ethanol aqueous solution for the third time, boil and reflux for 3 hours, collect the extract; combine the extracts obtained three times, and depressurize at 60°C Concentrate until there is no alcohol smell, the volume of the concentrated solution is 1 times the volume of the medicinal material, and the precipitate is removed by centrifugation;

[0028] 2. Degreasing: Extract the centrifuged medicinal liquid with 2 times the amount of petroleum ether in 3 times;

[0029] 3. Extraction: the degreased moth...

Embodiment 3

[0032] Embodiment 3: Extraction method of astilbin and dihydroquercetin

[0033] 1. Extraction: Grind the dried yellow bark leaves into coarse powder, add 12 times the amount of 80% ethanol aqueous solution for the first time for reflux extraction, reflux extraction for 4 hours after boiling, and collect the extract; add 5 times the amount of 50% ethanol for the second time The aqueous solution was boiled and refluxed for 5 hours, and the extract was collected; the two extracts were combined, concentrated under reduced pressure at 60°C until there was no alcohol smell, the volume of the concentrate was twice the volume of the medicinal material, and the precipitate was removed by centrifugation;

[0034] 2. Degreasing: Extract the centrifuged medicinal solution twice with 4 times the amount of petroleum ether;

[0035] 3. Extraction: extract the degreased mother liquor with 4 times the amount of ethyl acetate in 4 times, combine the ethyl acetate layers, concentrate under redu...

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Abstract

The invention relates to a method for extracting astilbin and dihydroquercetin from engelhardia roxburghiana wall leaves and application of extractives thereof. The method for extracting the astilbin from the engelhardia roxburghiana wall leaves comprises the steps of: extracting by refluxing ethanol, degreasing by using ligarine, extracting by using ethyl acetate, blending two organic solvents with different polarities in proportion, and performing recrystallization so as to obtain the astilbin; and the method for extracting the dihydroquercetin from the engelhardia roxburghiana wall leaves mainly comprises the steps of: firstly, obtaining the astilbin by using the provided method, and then hydrolyzing the astilbin to obtain the dihydroquercetin. The methods provided by the invention are beneficial to simplification of the traditional operation processes such as resin absorption, polyamide column or silicagel column chromatography and the like, and have the characteristics of simple, convenient and feasible process, simplicity for operation, lower cost and higher extraction efficiency and content; and after being measured with the HPLC (High Performance Liquid Chromatography) method, the contents of the astilbin and the dihydroquercetin are both more than 90%, therefore the method is suitable for industrialized production.

Description

technical field [0001] The invention relates to a method for extracting and separating astilbin and dihydroquercetin from the leaves of the plant Huangqi (Engelhardia roxburghiana Wall). Background technique [0002] Engelhardia roxburghiana Wall, a plant of the genus Huangqi in the family Juglandaceae, is a commonly used Chinese herbal medicine in my country. It is mainly distributed in Guangdong, Guangxi, Yunnan and other provinces. , damp heat jaundice, hernia abdominal pain. Huangqi leaves are often used as tea, which has many functions such as inhibiting obesity, anti-inflammation, anti-allergic reaction, lowering blood sugar, preventing high blood pressure and cancer. Astilbin is one of the main active ingredients isolated from Huangqi, which has significant antibacterial, anticancer, and hypoglycemic activities; in the cardiovascular system, it not only has hypolipidemic and anti-atherosclerotic effects, but also has myocardial protection. effect. In addition, astil...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08C07D311/32
Inventor 季红袁牧
Owner 广州医学院
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