14 results about "Mustard Greens" patented technology

The invention belongs to the technical field of molecular biology and plant molecular breeding, and particularly relates to a mustard core SNP molecular marker set and a screening method and application thereof. The invention provides a core SNP marker set (33) which is subjected to whole genome re-sequencing screening and experimental verification and is suitable for mustard germplasm resource identification and genetic analysis for the first time. The marker set is high in polymorphism and good in stability, covers the whole genome and can effectively distinguish different leaf mustard germplasms. By utilizing the core marker set and the matched KASP primer, the genetic typing of the leaf mustard germplasm resources can be quickly and accurately performed with high throughput, and the defects that the traditional morphological identification is time-consuming, labor-consuming and poor in accuracy are overcome. The marker set can be used for analyzing the genetic diversity, the population structure and the phylogenetic relationship of the leaf mustard germplasm resources, and a molecular basis is provided for collection, preservation and evaluation of the germplasm resources and breeding parent matching.

The invention discloses a brassica plant drought resistance negative regulation factor BnaA9.TZF5 and application of the brassica plant drought resistance negative regulation factor BnaA9.TZF5. The BnaA9.TZF5 is a transcription factor gene for coding CCCH type zinc finger protein in brassica napus, and a knockout plant obtained by performing targeted editing on the BnaA9.TZF5 through CRISPR / Cas9 shows that the drought resistance is remarkably enhanced along with phenotypic changes such as increase of leaf epidermis wax deposition and reduction of water loss rate; and an overexpression plant of the gene shows that the wax is reduced, the water loss is accelerated and the drought resistance is weakened, so that the BnaA9.TZF5 is a negative regulation factor for the drought resistance of the brassica plant. The BnaA9.TZF5 gene expression is knocked out to improve the drought resistance of brassica plants, the BnaA9.TZF5 gene can be used for molecular breeding of crops such as brassica napus, Chinese cabbage, cabbage and mustard, and a novel drought-resistant improved target gene is provided.

The invention provides a self-adaptive adjustment cabbage head leaf removing device and a cabbage head harvester. The problem that self-adaptive adjustment cannot be carried out according to crop sizes and irregular shapes in the prior art is solved. Comprising a mounting frame, a plurality of adjusting assemblies, an inner cylinder, a flexible assembly and a driving assembly, each adjusting assembly comprises a vertical plate and at least one self-adaptive adjusting part, and the vertical plate is connected with the side wall of a mounting through cavity through the at least one self-adaptive adjusting part; the inner cylinder is located in the mounting through cavity, and a leaf conveying and removing channel is jointly formed between the outer wall of the inner cylinder and the inner wall of an outer cavity defined by the vertical plates of the multiple adjusting assemblies; the flexible assembly comprises first flexible parts and second flexible parts, a plurality of first flexible parts are arranged on the outer wall of the inner cylinder, and a plurality of second flexible parts are arranged on the inner wall of the outer cavity; the driving assembly is used for driving the inner cylinder or the mounting frame to rotate so as to drive the cabbage heads to be conveyed and defoliated from the input end to the output end of the conveying and defoliating channel. The device not only can adapt to cabbage heads with different sizes, but also can remove leaves without damage.

The present utility model relates to a feed formulation for ZAMPEN native chicken (Gallus gallus domesticus) hardening stage characterized by comprising of a basal diet formulation and supplemental feed formulation made from fermented forages. The basal diet formulation comprises: of 1 wt.% molasses, 0.1 wt.% vitamins and minerals, or a combination selected from the group comprising: energy-source component of 4.75 wt.% cassava, 7 wt.%, corn bran, 50 wt.% corn yellow; a crude protein component of 3.9 wt.% copra meal, 20 wt.% fish meal and 12 wt.% soybean meal; and a calcium source component of 0.35 wt.% eggshell, 0.9 wt.% limestone. Whereas the supplemental feed formulation comprises: tricanthera, azolla, duckweed, and vegetable trimmings of pechay (Brassica rapa), mustasa (Brassica juncea), alugbati (Basella alba), kangkong (Ipomoea aquatica), and camote tops (Ipomoea batatas). The said formulation aims to significantly enhance feed intake of the native chicken while reducing feed costs.

This invention discloses an anti-porcine reproductive and respiratory syndrome virus (PRRSV) preparation and its preparation method. The anti-PRRSV preparation comprises traditional Chinese medicine, excipients, and probiotics. The traditional Chinese medicine includes: mustard greens, thistle, bitter melon, and barley sprouts. The excipients include: soybean meal, wheat bran, and molasses. The probiotics include: Bacillus subtilis, Enterococcus faecium, and Saccharomyces cerevisiae. By feeding pigs suffering from PRRSV with the anti-PRRSV preparation of this invention, PRRSV can be antagonized, improving the recovery rate of PRRSV-affected pigs and reducing the mortality rate. The anti-PRRSV preparation of this invention can be used as an antagonist of PRRSV. Furthermore, the anti-PRRSV preparation of this invention can also be used as a feed additive for the prevention or treatment of PRRSV in pigs.

This invention discloses a stem-nodular mustard BjuUbp15 This invention relates to genes and their encoded proteins and their applications, belonging to the field of plant genetic engineering technology. This invention cloned *Strombyx mori* using homologous cloning. BjuUbp15 Genes. Using Agrobacterium-mediated transformation, ... BjuUbp15 Genetic transformation of stem mustard with a gene overexpression vector yielded overexpressing plants. Experimental results showed that... BjuUbp15 The tuberous stems of the gene-overexpressing plants were significantly larger than those of the wild type. Therefore, the tuberous stem mustard of this invention... BjuUbp15 The gene has a positive regulatory effect on the size of the tuberous stem of *Mustela stenoptera*. Therefore, the present invention... BjuUbp15 Genes have great potential for increasing the yield of stem mustard.

The invention discloses a brassica juncea KASP molecular marker combination and application thereof in germplasm resource identification and breeding, and belongs to the technical field of molecular markers. The mustard KASP molecular marker set is developed from 193 mustard germplasm resources based on whole genome re-sequencing, the mustard KASP molecular marker can quickly, accurately and effectively identify and evaluate germplasm resource materials or bred varieties, and the problems of resource redundancy, inconvenient storage and management, intellectual property disputes and the like caused by accumulation of a large amount of germplasm can be solved. The molecular marker is provided for genetic map construction, gene positioning and molecular assisted breeding.

The invention belongs to the technical field of food additives, and particularly relates to shepherd's purse essence as well as a preparation method and application thereof. According to the capsella bursa-pastoris essence provided by the invention, all the raw materials are compounded together, and the ideal capsella bursa-pastoris essence effect is achieved by adjusting a reasonable proportion. The capsella bursa-pastoris essence has the characteristic flavor of capsella bursa-pastoris, the fragrance has the characteristic of being natural, soft and vivid, the flavor of the capsella bursa-pastoris is enriched, and the product quality is improved. The capsella bursa-pastoris essence disclosed by the invention is fresh in taste and lasting in fragrance. The preparation method is simple and does not need complex equipment.

The invention relates to the technical field related to plant genetic engineering, in particular to application of brassica juncea BjuWRKY75 in clubroot resistance of brassica napus L. The BjuWRKY75 gene (the CDS sequence of the BjuWRKY75 gene is as shown in SEQ ID NO.1) is successfully cloned from a clubroot-resistant brassica juncea L. material R13, a 2 * 35S overexpression vector is constructed, and the brassica juncea L. clubroot-resistant brassica juncea L. clubroot-resistant brassica juncea L. clubroot-resistant BjuWRKY75 gene is obtained. The gene is introduced into brassica napus by using an agrobacterium-mediated genetic transformation technology, and experiments prove that the over-expression of the gene can significantly improve the resistance of the brassica napus to the clubroot No.4 physiological race, which shows that the number of tumors at the root of a transgenic plant is significantly reduced; therefore, a brand new gene resource and an effective breeding way are provided for overcoming the technical bottlenecks that the existing brassica napus anti-clubroot resources are rare and the resistance source is single.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LT303 and application thereof.The lactobacillus plantarum strain LT303 is separated from an acid material, and a fermentation product obtained after fermentation of the strain and leaf mustard has the antibacterial effect on salmonella enteritidis, staphylococcus aureus and / or listeria monocytogenes; the content of sweet amino acids, namely threonine, alanine, serine, glycine and proline, of the pickled Chinese cabbages prepared by fermentation of the strain LT303 is remarkably high, and the fermented pickled Chinese cabbages are the most sweet and fresh in taste and the most abundant in sweet amino acid content; besides, the texture of the pickled Chinese cabbages prepared by adding the mango juice, the pitaya juice and the siraitia grosvenorii juice is enhanced, and the content of sweet amino acids is more remarkably increased, so that the LT303 strain can be used for preparing the pickled Chinese cabbages which are more sweet in taste and more unique in flavor, fermentation liquor has the value of researching and developing antibacterial peptides, and new raw materials and thoughts are provided for increasing the additional value of products in the later period.

This utility model discloses a windproof and lodging-preventing support device for planting dried mustard greens, belonging to the field of agricultural planting auxiliary technology. It includes a main support rod, an adjusting rod, a base, adjusting legs, and multiple fixing components. The base with spiked claws is fixed to the bottom of the main support rod, and the adjusting legs with spiked claws are hinged to the rear. The main support rod and the adjusting rod are connected by locking fasteners. Both have adjusting grooves on their surfaces, and the fixing components slide within these grooves to secure the dried mustard green stems. This device, with its adjustable height support structure and multiple sets of sliding fixing components, can adapt to the support needs of dried mustard greens at different growth stages, effectively preventing lodging, improving planting stability, and is convenient to install and adjust, making it highly practical.

The invention relates to the technical field related to gene engineering, in particular to application of brassica juncea BjuB01. JAL34 in clubroot resistance of brassica napus. The CDS sequence of the gene BjuB01. JAL34 is as shown in SEQ ID NO.1, and the coded amino acid sequence of the gene BjuB01. JAL34 is as shown in SEQ ID NO.2. The invention also relates to application of the gene BjuB01. JAL34 in clubroot resistance of brassica napus. According to the invention, the gene is over-expressed in a model plant arabidopsis thaliana and an important commercial crop brassica napus through a genetic transformation technology, so that the resistance of the gene to plasmodiophora brassicae No.4 physiological race can be remarkably improved; the BjuB01. JAL34 gene and the protein coded by the BjuB01. JAL34 gene can be used for culturing broad-spectrum and durable clubroot-resistant brassica napus and other new species of cruciferae crops, and have important theoretical value and wide application prospect.

The invention discloses a fermentation method of serofluid pickled Chinese cabbages, and belongs to the technical field of bioengineering fermentation. According to the method, lactobacillus plantarum and Pichia fermentans are used for synergistically fermenting the slurry-water pickled Chinese cabbages, compound inoculation is carried out according to the ratio of (1: 10)-(1: 1), and fermentation is carried out at the temperature of 30 DEG C for 5 days. According to the method, the accumulation of organic acids in the serofluid pickled Chinese cabbages is promoted through the interaction between the fermented pichia pastoris and the lactobacillus plantarum. Compared with single strain fermentation, the variety and coordination of volatile flavor substances in the synergistic fermentation serofluid pickled Chinese cabbage are obviously improved, and esters, ketones and phenols in the serofluid pickled Chinese cabbage are obviously accumulated. The method is beneficial to shortening the fermentation period of the serofluid pickled Chinese cabbage and improving the flavor quality of the serofluid pickled Chinese cabbage.

The utility model discloses a portable tribute vegetable airing clamp, including pole body one and pole body two, pole body one and pole body two set into same specification size, pole body two top fixedly connected with two rows of fixed rods, and pole body one is tightly attached in the top of fixed rod and uses the rope to fix tightly the both ends of pole body one and pole body two, through the design of multiple rows of fixed rods on pole body two, can directly be inserted in the fixed rod on tribute vegetable one end, then through the rope, pole body one and pole body two are assembled and fixed, can realize that multiple tribute vegetables are hung on the steel pipe support in disposable batch, when collecting vegetables, after taking down airing clamp one by one, unfasten the rope and take down the airing tribute vegetable, need no longer hang and air by the laborer, reduce its labor force and can improve its airing speed, especially applicable to the scene of tribute vegetable harvest volume.