29 results about "Cucumber seed" patented technology

The invention discloses a cucumber 1K liquid phase breeding chip and a special single nucleotide polymorphism variation site thereof. The 1K liquid phase breeding chip provided by the invention comprises a probe combination for detecting 1121 single nucleotide polymorphism variation sites in a cucumber genome, and the position information of a probe corresponding to each single nucleotide polymorphism variation site in a cucumber reference genome is shown in a table 3 in the specification. Single nucleotide polymorphism variation sites involved in the liquid phase breeding chip provided by the invention have the advantages of high polymorphism, good repeatability, stable and reliable marking, convenient statistics and the like, and a DNA fingerprint database for identifying the authenticity of cucumber varieties can be established based on the liquid phase breeding chip. The method can be used for cucumber variety authenticity, early breeding and identification of cucumber varieties and identification of cucumber seed resources, provides technical support for enrichment of cucumber germplasm resources, breeding of new varieties and protection of the new varieties, and has a wide application prospect.

The invention discloses a bacillus velezensis strain and application thereof. The invention discloses bacillus velezensis WLH (bacillus velezensis), and the preservation number of the bacillus velezensis WLH is CCTCC (China Center for Type Culture Collection) NO: M 20251238. The bacillus velezensis WLH is screened from rhizosphere of cucumber, the WLH is a biocontrol bacterium, can inhibit growth of pathogenic bacteria of cucumber root rot and fusarium wilt by degrading phenolic acid in soil and promote growth of cucumber plants, and the strain can relieve autotoxicity caused by the phenolic acid, inhibit the cucumber root rot and fusarium wilt and promote growth of cucumber plants. The promotion of crop growth comprises the capability of producing indoleacetic acid (IAA) and the capability of solubilizing phosphate, the promotion of germination of cucumber seeds under the stress of mixed phenolic acid and pathogenic bacteria, and the promotion of growth of cucumber seedlings under the stress of mixed phenolic acid and pathogenic bacteria. Soil continuous cropping obstacles generated in cucumber planting are effectively relieved, and a new basis is provided for microbial control of continuous cropping obstacles.

This invention proposes a high-nutrient-density multigrain composite low-temperature slow-fermentation dough, relating to the food industry. It comprises dry powder and water accounting for 50-60% of the dry powder's mass. The dry powder, by weight, includes the following ingredients: 400-500 parts high-gluten flour, 20-30 parts tricolor quinoa flour, 20-30 parts oat flour, 20-30 parts brown rice flour, 20-30 parts black rice flour, 1-2 parts sunflower seed flour, 1.5-5 parts pumpkin seed flour, 1.5-5 parts black sesame powder, and 1-5 parts cucumber seed flour. This formula contains whole grains such as quinoa flour, oat flour, and brown rice flour, retaining the bran and germ structure of the grains. It has high nutritional value and a low glycemic index.

This invention provides a traditional Chinese medicine composition and its application, belonging to the field of biomedical technology. The traditional Chinese medicine composition includes chicory, Tian Shan violet, fennel, rhubarb, dodder seed, chicory seed, ox tongue grass, cucumber seed, watermelon seed, rose petals, and mare's milk raisins. This composition is a targeted, dialectical formula for clearing the liver and promoting bile secretion. Its core advantage lies in its precise alignment with the physical characteristics of people in southern Xinjiang. Through the clever combination of four distinctive ingredients—cucumber seed, watermelon seed, rose petals, and mare's milk raisins—it achieves a high degree of compatibility between efficacy and constitution. This composition adheres to the core efficacy of clearing the liver and promoting bile secretion while also addressing the needs of people in southern Xinjiang for moisturizing, regulating qi, and protecting the spleen and stomach. This precise match between efficacy and constitution makes it a high-quality formula suitable for the people of southern Xinjiang and highly practical.

This utility model relates to the field of seed screening technology, and in particular to a cucumber seed screening and filtering device, comprising a filter box. Hydraulic rods are fixedly connected to the top left and right sides of the filter box. A connecting plate is fixedly connected to the output end of each hydraulic rod. A screen is fixedly connected to the end of the connecting plate away from the hydraulic rod. A drive motor is fixedly connected to the middle of the left side of the bottom of the filter box. A linkage shaft is rotatably connected to the middle of the right side of the lower part of the filter box. Through the design and cooperation of the hydraulic rods and the stepper motor, the hydraulic rods drive the screen in the filter box to slide up and down, supporting the cucumber seeds soaking in water and preventing the sunken seeds from falling to the bottom of the filter box. The stepper motor drives the screen located at the top of the filter box to move left and right cyclically inside the filter box, facilitating the quick retrieval of empty and shriveled seeds floating on the water surface, thus improving the efficiency of cucumber seed soaking and screening.

The invention belongs to the technical field of crop disease prevention and control, and particularly relates to a planting method for preventing cucumber fruit blotch germs and green mottle viruses, which comprises the following steps: acquiring soil and crop historical pathogen data, environment monitoring data and cucumber seed batch information of a planting area; querying supply chain quarantine records of the seeds, and carrying out standardization treatment on the seeds; after planting, the disease probability of each growth stage is predicted through a risk assessment algorithm, a dynamic monitoring plan is generated in combination with a fuzzy mapping algorithm, plant samples are collected, PCR detection of melon bacterial fruit blotch and RT-PCR detection of cucumber green mottle mosaic virus are carried out, and a quarantine report is issued. Based on the monitoring plan, the quarantine report and the real-time environment data, judging a comprehensive risk level, triggering a corresponding field intervention scheme, and recording an execution result to form an intervention log; after planting is finished, a prevention and control efficiency report is generated. Therefore, the problems of insufficient disease detection, lack of intervention measures and the like in the prior art are solved.

The invention relates to the field of plant molecular biology, in particular to application of a CsERF113L gene or a biological material containing the CsERF113L gene in regulation and control of stress resistance and yield of cucumbers. The cDNA (complementary deoxyribonucleic acid) sequence of the CsERF113L gene is as shown in SEQ ID NO. 1. The CsERF113L gene is knocked out in cucumber by adopting a CRISPR-Cas9 gene editing technology, the antioxidant capacity and photosynthetic capacity of cucumber plants are remarkably enhanced, the saline-alkaline resistance is remarkably improved, the root growth is accelerated, the cucumber yield is remarkably increased, and knockout strains and over-expression strains have opposite trends. The result shows that the CsERF113L gene affects the regulation and control mechanism of cucumber stress resistance. The CsERF113L gene has important guiding significance and application value for guiding widening of cucumber cultivation conditions in actual production, improvement of cucumber high yield and stress resistance and cucumber seed selection and breeding.

The invention discloses a liver-protecting traditional Chinese medicine composition as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The liver-protecting traditional Chinese medicine composition comprises a traditional Chinese medicine aqueous extract and fruit vinegar, and the traditional Chinese medicine comprises the following components in parts by weight: 2-10 parts of cichorium glandulosum seeds, 10-20 parts of fennel, 2-10 parts of celery seeds, 2-10 parts of white rapeseeds, 2-10 parts of melon seeds, 2-10 parts of watermelon seeds, 2-10 parts of cucumber seeds, 2-10 parts of purslane seeds and 10-20 parts of cichorium glandulosum roots. The traditional Chinese medicine composition disclosed by the invention can intervene in liver injury through a multi-target and multi-channel synergistic effect, and has good anti-hepatitis and anti-hepatic fibrosis effects.

The invention discloses a cucumber CsCEPR1 gene, a promoter and application of the cucumber CsCEPR1 gene in regulating and controlling the size of cucumber seeds. The CsCEPR1 gene of the cucumber is identified and confirmed for the first time, and it is found that the expression level of the CsCEPR1 promoter is reduced due to deletion of a 29bp basic group on the CsCEPR1 promoter, so that the sizes of seeds and fruits of the cultivated cucumber are increased. The method comprises the following steps: constructing a transgenic vector pCAMBIA1305.4-CsCEPR1 of a CsCEPR1 gene, transforming a plasmid into a cucumber to obtain a transgenic plant of the CsCEPR1, carrying out field selfing pollination, molecular identification and phenotype identification to prove that the CsCEPR1 gene has the function of regulating and controlling the sizes of cucumber seeds and fruits, and when the expression of the CsCEPR1 gene is enhanced, the cucumber seeds are obviously reduced and have serious dormancy, and the fruits are obviously shortened.

This invention discloses a method for regulating cucumber seed germination at low temperatures. CsGAI Mutated genes and their applications. This invention constructs a targeted gene... CsGAI The gene editing vector pCas9CsGAI was used to transform cucumbers, and the resulting plasmid was then transformed into cucumbers. CsGAI Genetically modified plants were used for field hybridization and pollination, molecular identification, and phenotypic identification to prove that when CsGAI CsGAI CsGAI CsGAI CsGAI CsG When gene function is lost or weakened, the relative germination rate, relative germination potential, and relative germination index of cucumbers increase, and their cold tolerance improves. This invention lays the foundation for revealing the genetic and biological mechanisms of cold tolerance during cucumber germination, provides new gene resources for the cultivation of cold-tolerant cucumbers, and is of great significance for cucumber cold research and the breeding of new cold-tolerant varieties.

The invention belongs to the technical field of crop cultivation and breeding, and particularly discloses a system and method for predicting the germination rate of cucumber seeds based on a hyperspectral imaging technology. The method comprises the following steps: acquiring materials in Dai nationality autonomous prefecture in Xishuangbanna of Yunnan province, performing seed reproduction in a specified base to obtain experimental seeds, performing serial number scanning on the seeds harvested in 2021-2023 to obtain spectral information, and acquiring images with the wavelength of 400-1000nm by using a specific hyperspectral imaging system. The method comprises the following steps: constructing a data set after marking the germination condition through a germination experiment, preprocessing a spectrum by applying at least one method such as multivariate scatter correction, extracting a characteristic wavelength by adopting an algorithm such as non-information variable elimination, predicting by virtue of a KNN or LogitBoost model, and evaluating by using indexes such as accuracy and the like, thereby effectively predicting the germination rate of the Xishuangbanna cucumber seeds.

The invention discloses a KASP marker closely linked with a cucumber fruit length regulation gene CsERF1a and application of the KASP marker. The SNP primer combination provided by the invention comprises a first primer group and / or a second primer group, each primer group consists of three primer sequences and is used for amplifying an SNP (Single Nucleotide Polymorphism) site; the nucleotide sequences of the primers are sequentially shown as SEQ ID NO: 1 to SEQ ID NO: 6. The SNP primer combination can be used for early identification in the seed or seedling stage of cucumbers, so that screening of materials of different fruit length types is accelerated, and technical support is provided for cucumber fruit length breeding. The method provided by the invention has the advantages of high throughput, accuracy, low cost, simplicity in operation, manpower and material resource saving and the like, and has a very wide application prospect.

The invention discloses a cucumber seed coating agent and a seed coating method, and belongs to the technical field of cucumber coating. Specific materials are selected as coating agent components to coat cucumber seeds, and multi-layer coated seeds are prepared; the coating agent can realize a phosphorus-nitrogen release sequence to accurately and dynamically match nutrient requirements of cucumber seeds in different stages after sowing, so that the emergence rate of the cucumber seeds is improved, good growth of root systems and overground parts is promoted, cucumber seedlings with good growth conditions are obtained through cultivation, and the coating agent has good application prospects in the technical field of cucumber cultivation.

The invention relates to the technical field of application of microorganisms, in particular to a phosphate solubilizing bacterium BPC41 and application thereof. The salt-tolerant bacteria are separated from the saline-alkali soil by adopting a soil dilution coating method and a plate streaking method, and the salt-tolerant bacteria BPC41 are separated and identified from the Tangshan saline-alkali soil by re-screening based on the phosphate solubilizing capacity of the salt-tolerant bacteria. A comprehensive characteristic determination result shows that the strain has excellent saline-alkali tolerance and a multi-element growth-promoting function, specifically, the strain can efficiently decompose organic phosphorus, dissolve inorganic phosphorus and fix nitrogen, and meanwhile has the growth-promoting characteristics of secreting indoleacetic acid and forming a biological membrane. A germination test result shows that the BPC41 strain not only can promote germination of red bean seeds, lettuce seeds, cucumber seeds, wheat seeds and watermelon seeds, but also can promote growth of soybean seed buds under salt stress and relieve salt stress. Pot experiment results show that the plant growth can be promoted under the saline-alkali stress condition.

The present invention belongs to the field of crop planting technology and specifically relates to the use of a silver-silicon nanocomposite material to promote cucumber seed germination and growth under drought stress. The synthesized Ag@SiO2 NPs of the present invention have the advantages of simple process, low cost, high yield, and good material dispersibility. They can significantly promote cucumber seed germination and growth under drought stress, are simple to operate, have significant effects, are low cost, and are easily disseminated.

The invention relates to the technical field of functional food, and particularly discloses composite dietary therapy powder for improving intestinal motility disorder during pregnancy and a preparation method thereof, and the composite dietary therapy powder comprises the following components: composite nutrient fibers, cucumber seeds, sweet almonds, black sesames, red dates, banana composite slices, carrots, crystal sugar and a nutritional microbial agent; wherein the added composite nutrient fibers are subjected to enzymolysis through raw materials such as lily bulbs and the like, and then standing fermentation is performed, so that macromolecules and anti-nutrients can be decomposed, and the content of active ingredients is increased; the added nutritional microbial agent is decomposed through oligosaccharide and casein to form a load system, the strain is pre-activated to improve the activity and increase the content of active substances such as exopolysaccharides, the proliferation activity of probiotics is high, and the intestinal health is improved; the added banana composite slices decompose tannic acid through a process, and the content of oligosaccharide and other substances is increased; according to the invention, multiple nutritional ingredients take effect synergistically, the proportion of the ingredients is regulated and controlled, and the intestinal motility disorder of pregnant women during pregnancy is comprehensively improved from the aspects of hormone regulation, flora regulation, nutrition supplement and the like.

The invention discloses a soybean compound fermentation composition for treating climacteric syndrome as well as a preparation method and application thereof, relates to the technical field of special medical foods, and aims to solve the problem that a composition for treating climacteric syndrome, which is high in bioavailability and remarkable in effect, is lacked in the prior art. The preparation method is technically characterized by comprising the following steps: 1, respectively preparing a ginseng liquid medicine, a rehmannia liquid medicine, a rhizoma polygonati liquid medicine and a radix puerariae liquid medicine; 2, respectively adding soybeans into each liquid medicine, soaking until the liquid medicine is completely absorbed, sterilizing, respectively inoculating fermentation strains for fermentation, drying and crushing to obtain a ginseng and fermented soybean compound fermented product, a rehmannia and fermented soybean compound fermented product, a sealwort and fermented soybean compound fermented product and a kudzu vine root and fermented soybean compound fermented product; 3, soaking rhizoma polygonati and goat milk for multiple times, steaming, fermenting and drying to prepare a milk rhizoma polygonati fermented product; and 4, crushing and mixing the compound fermented product, the milk sealwort fermented product and the cucumber seeds to obtain the soybean compound fermented composition for treating the climacteric syndrome.

The application discloses a cucumber seed oil extract, a preparation method and application thereof, cosmetics and medicines. The method comprises the following steps: providing dried cucumber seeds; seed oil extraction, comprising squeezing the dried cucumber seeds or performing supercritical carbon dioxide extraction after crushing to obtain crude cucumber seed oil; seed oil purification, comprising removing water-soluble organic acids in the crude cucumber seed oil to obtain an oil phase liquid; deodorization, comprising deodorizing the purified oil phase liquid to obtain deodorized crude cucumber seed oil; degreasing, comprising removing solid esters in the deodorized crude cucumber seed oil to obtain degreased crude cucumber seed oil; and impurity removal and drying of the degreased crude cucumber seed oil to obtain the cucumber seed oil extract. The preparation method of the cucumber seed oil extract is capable of obtaining the cucumber seed oil extract through drying, seed oil extraction, seed oil purification, deodorization, degreasing, impurity removal and drying in sequence, and has the advantages of simple operation, high yield, low cost, stable product quality, green and environment-friendly process and suitability for large-scale production.

The application discloses a cucumber 1K liquid-phase breeding chip and a single nucleotide polymorphism variation site special for the same. The 1K liquid-phase breeding chip provided by the application comprises a probe combination for detecting 1121 single nucleotide polymorphism variation sites in a cucumber genome, and the position information of the probe corresponding to each single nucleotide polymorphism variation site in the cucumber reference genome can be seen from Table 3 in the specification. The single nucleotide polymorphism variation site involved in the liquid-phase breeding chip provided by the application has the advantages of high polymorphism, good repeatability, stable and reliable marker, convenience in statistics and the like. The application can also establish a DNA fingerprint database for identifying the authenticity of the cucumber variety based on the liquid-phase breeding chip, and can be used for the authenticity of the cucumber variety, early selection and identification of the cucumber variety, identification of the cucumber seed resource, and provides technical support for the enrichment of the cucumber germplasm resource, selection of new varieties and protection of new varieties, and has a wide application prospect.

The invention discloses trichoderma asperellum YB02, a fungus residue organic fertilizer prepared from the trichoderma asperellum YB02 and application of the trichoderma asperellum YB02 to prevention and treatment of fusarium oxysporum, belongs to the technical field of microorganisms, and particularly relates to trichoderma asperellum YB02 which is preserved in China General Microbiological Culture Collection Center on December 24, 2025, the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.3687. The preservation number is CGMCC (China General Microbiological Culture Collection Center) The invention relates to application of trichoderma asperellum YB02 in preparation of a preparation for preventing and treating fusarium oxysporum. The trichoderma asperellum YB02 can be used for preventing and treating fusarium oxysporum. The invention discloses trichoderma asperellum YB02, the trichoderma asperellum YB02 is used for preparing a mushroom dreg organic fertilizer, amplification propagation of fusarium oxysporum in the mushroom dreg organic fertilizer can be effectively inhibited, and the prepared mushroom dreg organic fertilizer can promote germination of cucumber seeds and effectively prevent and treat cucumber fusarium wilt.

The present application relates to the technical field of traditional Chinese medicine, and provides a traditional Chinese medicine composition capable of promoting bone fracture healing, which comprises the following components in parts by weight: Cordyceps militaris 25-35 parts, raspberry 20-25 parts, cucumber seed 15-22 parts, perilla seed 12-18 parts, radish seed 10-15 parts, bovine collagen peptide powder 8-12 parts, angelica 5-10 parts, and safflower 1-5 parts. The technical scheme solves the problem of poor effect of traditional Chinese medicine composition in promoting bone fracture healing.

The utility model provides a non-crushing type melon and fruit seed taking device, and belongs to the technical field of agricultural seed taking. By means of the structural arrangement that the seed taking pipe and the stirring frame rotate in the device shell, the rotating seed taking pipe can take seeds of cucumbers, the situation that the amount of cucumber seed taking waste is large is avoided, the pressure of waste treatment is reduced, the stirring frame can drive water flow to conduct stirring, and melon pulp, jelly and germs on the surfaces of the seeds can be conveniently removed; through the structural arrangement that the threaded rod rotates on one side of the fixing block, the threaded rod can drive the two adjusting blocks to move oppositely or oppositely, and then the device can be suitable for cucumbers of different sizes. The seed taking device comprises a device shell, a driving motor, a fixed block and a seed taking assembly, a cavity is formed in the device shell, a driving motor is fixedly installed on one side of the device shell through bolts, a fixing block is fixedly arranged on the top face of the device shell, and a seed taking assembly is arranged in the device shell and located below the fixing block.

The invention relates to a traditional Chinese medicine composition for promoting fracture healing. The traditional Chinese medicine composition is prepared from, by weight, 30-15 g of panax notoginseng leaves, 40-25 g of cucumber seeds, 20-12 g of eucommia ulmoides, 12-6 g of peach kernels, 15-7 g of smoked plums, 12-5 g of cloves, 11-4 g of cinnamon, 12-6 g of radix polygonati officinalis, 12-7 g of licorice roots and 30-20 g of impatiens balsamina. The medicine composition mostly adopts medicinal and edible non-toxic medicinal materials and non-scarce medicinal materials which are easy to plant and obtain, animal components are not used as medicines, a synergistic scheme is formed by optimizing compatibility and integrating the medicinal materials for activating blood, tonifying liver and kidney and strengthening muscles and bones, callus formation is promoted, pain is relieved and bone repair is accelerated through the synergistic effect, and the curative effect is good. Meanwhile, the medicine activates and awakens the strong recovery capability of a human body recovery system, and diseases are cured. The medicine formula is suitable for adjuvant therapy of various stages of fracture.

The utility model discloses a seedling raising device for cucumber seeds, and relates to the technical field of seedling raising devices. The seedling raising device for cucumber seeds comprises a seedling raising tray, a seedling raising tray and a seedling raising tray, wherein a plurality of seedling raising holes are formed in the seedling raising tray in a rectangular array; the seedling raising plate is elastically connected with the bottom plate; the hole plate is located in the seedling raising hole, and when the seedling raising tray is pressed downwards, the hole plate moves upwards in the seedling raising hole; when seedlings and soil in the seedling holes need to be taken out, only the seedling tray needs to be pressed downwards, so that the hole plate ascends in the seedling holes, the seedlings are jacked upwards, the seedlings are loosened in the seedling holes, and then the seedlings can be taken out of the seedling holes conveniently. Therefore, the situation that the seedlings are separated from the soil when the seedlings are taken out of the seedling holes is effectively avoided, the seedling taking quality of cucumber seedling raising is improved, meanwhile, the device can be repeatedly used, and the seedling raising cost is saved.

The invention relates to the technical field of microorganisms, and particularly discloses a bacillus P6 as well as a fungicide and application thereof, the bacillus P6 is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 15, 2025, the preservation number is CGMCC NO.34218, and the preservation number is CGMCC NO.34218; the bacillus P6 provided by the invention has an obvious growth-promoting effect on plants, which is mainly embodied in that the bacillus P6 has an obvious growth-promoting effect on germination of cucumber seeds, has an obvious growth-promoting effect on growth of apple seedlings and has an obvious growth-promoting effect on cherry seedlings at the same time.

The application discloses a microbial agent for promoting growth of cucumbers, a preparation method and application thereof, and belongs to the technical field of agricultural microorganisms. The microbial agent comprises the following components: denitrifying achromobacter, Bradyrhizobium elkanii and Azotobacter vinelandii, and the volume ratio of the three is 2:1:1. The bacteria with growth promotion are reasonably combined and used, so that the mixed bacterial flora of the application is superior to a single strain in terms of growth promotion effect on cucumbers, and the synergistic effect among the bacterial strains is more adaptable in the actual soil rhizosphere complex environment. Through determination of the number of bacterial strains and mixing of specific proportions, the growth of cucumber seeds is stably and effectively promoted, the root elongation is promoted, the flowering and fruit setting rate is improved, and the plant height, plant biomass and chlorophyll content of cucumber seedlings are improved. In addition, the microbial agent can help improve the growth quality and yield of plants, reduce environmental pollution, has wide application potential in the fields of agriculture and ecology, and is worth promoting.

This utility model provides a cucumber seed peeling device for processing cucumber seed powder, including a scraping mechanism, a drying mechanism, and a filtering mechanism. The drying mechanism is located at the lower end of the scraping mechanism, and the filtering mechanism is located at the left end of the scraping mechanism and the drying mechanism. The scraping mechanism includes a scraping conveyor belt, a scraping base plate, a scraping motor, a scraping air pump, a feed box, and scraping blades. The scraping conveyor belt is located at the upper end of the scraping base plate, and the scraping motor is installed on the front end of the scraping conveyor belt. This design solves the problem that in the original device, cucumbers are also crushed, resulting in too many cucumbers and cucumber seeds mixed together, leading to poor separation efficiency, significant waste, and high cucumber seed production costs. This utility model designs a scraping mechanism that can separate the scraped pulp from the cucumbers first, effectively improving the efficiency of subsequent cucumber seed separation, and the cucumbers can be retained more intact, reducing waste in production and lowering the production cost of cucumber seeds.

The invention provides a medicinal and edible nutritional composition for reinforcing and strengthening bones and application of the medicinal and edible nutritional composition in preparation of a bone nutrition preparation, the composition is composed of deer blood powder, peach gum, cucumber seeds, turmeric and epigallocatechin gallate, can improve serum osteocalcin, promote fracture healing and improve osteoporosis, and has nutritional effects of reinforcing and strengthening bones.

The invention relates to a cucumber seed specific promoter pSEP5 and application thereof. The invention further discloses an expression vector pCAMBIA2300-enhance35S-pSEP5-DsRed2-d35S-eGFP containing a candidate promoter pSEP5, the expression vector is characterized in that restriction enzymes BamHI and EcoRI are used for carrying out linear treatment on a vector pCAMBIA2300-enhance35S-LTP2-DsRed2-d35S-eGFP, the vector pCAMBIA2300-enhance35S-LTP2-DsRed2-d35S-eGFP is used for constructing a vector pCAMBIA2300-enhance35S-pSEP5-DsRed2-d35S-eGFP with fragments pSEP5 and fragments DsRed2 through a homologous recombination method The invention also discloses application of the cucumber seed specific promoter pSEP5 in driving expression of a cucumber SPT system seed fluorescent module and in cucumber hybrid seed production.