The invention discloses a glucosamine chondroitin collagen tablet which comprises raw materials and auxiliary materials. The raw materials contain the following ingredients, by weight, 175-185 parts of D-glucosamine hydrochloride, 195-205 parts of calcium carbonate, 80-90 parts of chondroitin sulfate, 55-65 parts of collagen and 0.4-0.5 part of vitamin D3. The auxiliary materials contain starch and magnesium stearate, and the total weight of the auxiliary materials is 170-180 parts. The invention also discloses a preparation method of the glucosamine chondroitin collagen tablet. The preparation method comprises steps as follows: receiving of raw materials, feeding, crushing, sieving, weighing, mixing, preparation of a soft material, pelletizing, drying, particle shaping, total blending, tabletting and the like. The glucosamine chondroitin collagen tablet provided by the invention is prepared by a scientific technological method according to a certain weight ratio. By the use of the glucosamine chondroitin collagen tablet, osteoporosis can be prevented and treated safely and effectively, and bone mineral density can be increased.