Bifidobacterium lactis-containing fermented dairy product and application thereof

A technology for fermented dairy products and Bifidobacterium lactis is applied to the application field of fermented dairy products containing Bifidobacterium lactis, so as to achieve the effects of improving immunity and preventing and treating osteoporosis.

Active Publication Date: 2020-04-07
INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, after investigation, there are few technical reports on the us

Method used

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  • Bifidobacterium lactis-containing fermented dairy product and application thereof
  • Bifidobacterium lactis-containing fermented dairy product and application thereof
  • Bifidobacterium lactis-containing fermented dairy product and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: Bifidobacterium lactis BL-99 and performance measurement thereof

[0045] The Bifidobacterium lactis BL-99 of the present invention is from Shanghai Jiaoda Only Co., Ltd., and is isolated from the intestinal tract of infants. The strain was preserved in China General Microorganism Culture Collection and Management Center CGMCC on April 26, 2018 (Address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences), taxonomic name: Bifidus lactis Bacillus (Bifidobacterium lactis); the deposit number is CGMCC No.15650.

[0046] 1. Taxonomic characteristics of Bifidobacterium lactis BL-99

[0047] Physical and chemical test results:

[0048]

[0049] 16S rRNA gene sequence sequencing results (SEQ ID No.1):

[0050] GCTCCCCCACAAGGGTCGGGCCACCGGCTTCGGGTGCTACCCACTTTCATGACTTGACGGGCGGTGTGTACAAGGCCCGGGAACGCATTCACCGCGGCGTTGCTGATCCGCGATTACTAGCGACTCCGCCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGACCGGTTTTCAGC...

Embodiment 2

[0170] Embodiment 2: the dairy product that contains the starter fermentation preparation of Bifidobacterium lactis BL-99

[0171] Preparation of Bifidobacterium lactis single-bacteria starter: refer to Figure 6 In the shown fermentation process, Bifidobacterium lactis BL-99 (that is, Bifidobacterium lactis with preservation number CGMCC No. 15650) is anaerobically cultured in TPY liquid medium. TPY liquid medium (g / L): hydrolyzed casein 10.0, soybean peptone 5.0, yeast powder 2.0, glucose 5.0, L-cysteine ​​0.5, dipotassium hydrogen phosphate 2.0, magnesium chloride 0.5, zinc sulfate 0.25, calcium chloride 0.15, ferric chloride 0.0001, Tween 80 1.0, pH 6.5±0.1. After primary, secondary and expanded culture, the fermentation broth was centrifuged at 4°C and 2500rpm for 10min to collect the bacteria. The collected bacteria were freeze-dried to obtain BL-99 active bacteria powder as a single-bacteria starter, and stored below -18°C.

[0172] Mix the above-mentioned single-bac...

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Abstract

The present invention discloses a bifidobacterium lactis-containing fermented dairy product and an application thereof, and specifically provides the fermented dairy product. The fermented dairy product contains bifidobacterium lactis with a preservation number of CGMCC No.15650. The bifidobacterium lactis with the preservation number of CGMCC No.15650 can be present in the fermented dairy productas an active bacterium or inactivated form. The fermented dairy product has effects of preventing osteoporosis, adjusting balance of intestinal flora, etc.

Description

technical field [0001] The invention relates to a fermented milk product containing probiotics and its application, in particular to a fermented milk product containing Bifidobacterium lactis and its application in preventing osteoporosis and the like. Background technique [0002] Osteoporosis is a systemic metabolic skeletal disease characterized by decreased bone mass and destruction of bone microstructure. It is manifested by increased bone fragility, which can easily induce fractures. About 200 million people worldwide suffer from osteoporosis. As the incidence of hip fractures due to osteoporosis is increasing worldwide, the elderly population is largely affected by fracture risk. The total number of people with disabilities worldwide is 5.8 million per year. Fifty-one percent of this figure was due to fractures, mainly in Europe and the Americas. Consequently, osteoporotic fractures are considered to be an important cause of mortality and morbidity in developed co...

Claims

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Application Information

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IPC IPC(8): A23C9/123C12N1/20C12R1/01
CPCA23C9/1234C12N1/20A23V2400/531
Inventor 洪维鍊刘伟贤赵雯孙婷何剑刘红娟
Owner INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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