75 results about "Beneficial bacteria" patented technology

The application discloses a method for continuously fermenting urine to produce safe fertilizer, which comprises the following steps: in the first fermentation, adding environment-friendly enzyme into a container, then collecting fresh urine and adding molasses, and carrying out fermentation in a sealed manner; after the fermentation is completed, reserving part of the fermentation liquor as a substrate; and in the subsequent batches, directly adding fresh urine and molasses into the container with the substrate to carry out fermentation, so as to realize continuous circulation. The method can inhibit the hydrolysis process of urea in the urine in the early stage by timely adding environment-friendly enzyme (or the fermentation liquor of the last time) and molasses into the fresh urine to carry out fermentation, thereby greatly reducing the ammonia nitrogen volatilization amount, and the pathogenic bacteria in the urine can be inhibited to a very low level in a short period of time. Except that the environment-friendly enzyme needs to be added in the first fermentation, the environment-friendly enzyme does not need to be added in the subsequent batches of fermentation, and only a small amount of molasses needs to be added. A large amount of beneficial bacteria such as lactic acid bacteria and yeast and metabolic products such as lactic acid and acetic acid are generated in the fermentation process, so that the urine is converted into microbial bacterial fertilizer.

This invention discloses a method for extracting soluble dietary fiber from Polygonatum sibiricum residue, belonging to the field of traditional Chinese medicine extraction technology. This invention provides a method for extracting soluble dietary fiber from Polygonatum sibiricum residue with high yield, and discovers that this soluble dietary fiber can effectively resist oxidation, significantly improve the richness and uniformity of gut microbiota, improve gut microbiota composition imbalance, promote the proliferation of beneficial bacteria, inhibit the excessive growth of harmful bacteria, and play a protective role against intestinal damage by regulating the structure of intestinal microbiota and maintaining microecological stability, showing broad application prospects.

This application discloses a sheep milk probiotic formula milk powder that can regulate intestinal flora and its preparation method. By weight, it contains the following raw material components: 600-700 parts sheep milk base, 5-15 parts compound probiotic powder, 30-60 parts prebiotic composition, 10-30 parts functional additives, and 5-20 parts nutritional fortifiers. This compound formula combines multiple probiotics and optimizes the strain ratio to achieve a synergistic colonization effect. The probiotics are prepared using a freeze-drying process, effectively improving problems caused by flora imbalance. Simultaneously, multiple compound prebiotics provide nutritional support for the proliferation of beneficial bacteria, synergistically enhancing the intestinal regulatory effect. Added functional components such as lactoferrin, chitosan, β-glucan, and poria cocos polysaccharide make it suitable for long-term conditioning consumption by sub-healthy individuals and those with weak digestive systems. The raw sheep milk undergoes vacuum distillation to remove the muttony odor. A process of wet powdering followed by aseptic dry mixing of probiotics is used, resulting in a more stable overall quality of the finished product.

The application provides bifidobacterium longum MG-G763 for improving constipation, and belongs to the technical field of microorganisms. The bifidobacterium longum MG-G763 provided by the application can effectively relieve constipation by integrating multiple effects such as nerve regulation, immune regulation, barrier repair and flora regulation, and systematically restoring intestinal function homeostasis. The bifidobacterium longum MG-G763 can significantly improve the defecation particle number and defecation weight of constipated mice, shorten the time for defecating the first black stool, improve the small intestinal transport rate of the mice, effectively improve and relieve the defecation condition of the constipated mice, and significantly increase the amount of intestinal beneficial bacteria and reduce the amount of harmful bacteria, thereby promoting intestinal health.

This invention discloses a probiotic liquid core ball cultured under polysaccharide confinement, its preparation method, and its application in improving children's intestinal flora. Belonging to the field of probiotic preparation technology, it employs an extrusion method combined with calcium alginate cross-linking technology, using xanthan gum and other thickening polysaccharides to optimize the types and concentrations of polysaccharides to construct the confinement space. The effects of different oxygen conditions on the encapsulation of different proportions of *Lactobacillus rhamnosus* LRa05 and *Bifidobacterium animalis* BLa80 were investigated to obtain the optimal probiotic liquid core ball cultured under polysaccharide confinement. This process is simple, uses safe raw materials, and after confinement culture, the viable bacteria count is ≥10⁹ CFU / g. It can directionally increase the abundance of beneficial bacteria in children's intestines, inhibit opportunistic pathogens, enhance flora diversity and stability, and is suitable for children's dietary scenarios, providing a safe and efficient intestinal flora regulation solution.

A beverage containing dead beneficial bacteria and at least one of fruit juice and vegetable juice, the dead bacteria concentration of the beneficial bacteria being 5 hundred million / L or more.

The application discloses a brain hemorrhage prognosis improvement method based on intestinal flora regulation and relates to the technical field of intestinal microecology.The application comprises the following steps: detecting feces, starting intervention if the enterococcus abundance and neuroinflammatory metabolic markers exceed the standard, stopping the use of conventional nutrient solution, perfusing a special component containing sugar analogs, blocking the energy uptake of enterococcus, simultaneously providing a carbon source for beneficial bacteria, synchronously perfusing a microsphere suspension containing a molecular capture function, adsorbing and fixing proinflammatory metabolic waste in the intestinal tract, blocking the blood entry of the proinflammatory metabolic waste, perfusing live bacteria with different functions three times in time sequence, sequentially completing the acidification of the environment, constructing a bacterial membrane barrier and repairing the intestinal epithelium, and re-detecting after each round of intervention, selecting the maintenance period or adjusting the intensity and then recycling according to the decrease of enterococcus and markers.The application can limit the competitive advantage of enterococcus in the utilization of carbon sources after brain hemorrhage by sequentially implementing competitive inhibition of the phosphotransferase system and multi-species ecological niche occupation.

This invention discloses a plant Priestia megaterium This invention relates to strain H1-8 and its applications, belonging to the field of beneficial bacteria technology. The invention provides a strain... Priestia megaterium Strain H1-8 was deposited at the China Center for Type Culture Collection (CCTCC) on December 18, 2025, with accession number CCTCC M 20252958. This strain exhibits high salt and alkali tolerance; it has the ability to reduce salinity (12.56%); and it also possesses nitrogen-fixing, potassium-solubilizing, organic phosphorus-solubilizing, inorganic phosphorus-solubilizing, protease-producing, and IAA-producing growth-promoting abilities. Under both normal and salt-alkali stress conditions, it significantly promotes the growth of both above-ground and below-ground parts of cucumber plants, increasing cucumber yield and improving cucumber quality. Furthermore, it can significantly promote the growth of horticultural plants such as tomatoes.

The present application aims to provide a preparation method of rice bran fermented peptide for goose feed, which comprises the following steps: fermenting fresh rice bran to obtain an enzymatic fermentation compound, and feeding the goose with the enzymatic fermentation compound, so that the small peptides and intestinal beneficial bacteria produced can provide better nutrition, are easy to absorb, improve the disease resistance rate, and can avoid oxidation of the rice bran and prolong the storage period of the rice bran.

This invention discloses an integrated soil regeneration granule for indigenous bacteria activation and soil improvement, and its preparation method. The granule employs a two-component (A / B) layered structure, consisting of a disintegration trigger core, an A-component habitat layer, and a B-component functional layer from the inside out. The disintegration trigger core contains citric acid microcapsules coated with gelatin-gum arabic, which, in synergy with sodium bicarbonate, achieves precise gas production and disintegration over 12 months, propelling microbial communities and nutrients to deeper soil layers. The A-component habitat layer constructs a gradient porous structure, inhibiting pore blockage and efficiently capturing indigenous bacteria. The B-component functional layer relies on biochar and humic acid for nutrient supply, and incorporates oyster shell powder to construct a pH buffering system. This granule can both capture and colonize beneficial indigenous bacteria, and can also be artificially and directionally implanted with beneficial bacteria, serving as an excellent carrier for microbial fertilizers to enhance their efficacy. It achieves synergistic effects of saline-alkali soil improvement, microbial activation, and soil structure reconstruction, specifically addressing the shortcomings of similar technologies in Japan, and has a wider range of applications.

This invention relates to the field of knowledge graph technology, specifically disclosing a knowledge graph-based system for predicting gut microbiota colonization effects and recommending nutrients. The system includes: knowledge graph construction, data acquisition, microbiota game modeling, dynamic competitive evolutionary reasoning, Nash equilibrium calculation, colonization probability prediction, and a strategic nutrient recommendation output device. It identifies microbiota interactions by constructing a heterogeneous semantic network, describes the strategic change paths of the microbiota under resource conditions using evolutionary game theory algorithms, and identifies the system's stable state and risks by combining Nash equilibrium points. By introducing a dynamic competitive network based on evolutionary game theory, this invention can accurately predict the outcome of microbiota competition, avoid resource competition risks, guide the gut towards a stable state dominated by beneficial bacteria, and improve the accuracy, safety, and regulatory effect of nutritional intervention.

This invention belongs to the field of pharmaceutical technology, and relates to the use of a combination of sour cherry powder and celery seed powder, and complexes containing this composition. Specifically, the sour cherry powder and celery seed powder composition is used in the preparation of drugs for treating or regulating intestinal flora imbalance caused by hyperuricemia. The preparation method of this composition is as follows: pitting sour cherries, freezing them separately with liquid nitrogen, grinding them into powder using a pulverizer, and sieving them separately to obtain sour cherry powder and celery seed powder; mixing the obtained sour cherry powder and celery seed powder at a ratio of (0.5~3):1. This invention discusses the diversity, richness, and evenness of intestinal flora through diversity analysis, community bar chart analysis, and Venn diagram analysis. The results show that this composition can increase beneficial bacteria (Prevotella, Mulliez, Lactobacillus, etc.), reduce harmful bacteria (Alternaria), improve the structure of intestinal flora, and maintain the balance of intestinal flora.

This invention discloses a plant Bacillus marisflavi This invention relates to the ASR-60 strain and its applications, belonging to the field of beneficial bacteria technology. The invention provides a strain... Bacillus marisflavi The ASR-60 strain was deposited at the China Center for Type Culture Collection on July 14, 2025, with accession number CCTCC M 20251592. The strain exhibits high salt and alkali tolerance and the ability to reduce soil salinity by 18.67%. It also possesses growth-promoting abilities such as nitrogen fixation, potassium solubilization, organic phosphorus solubilization, inorganic phosphorus solubilization, protease production, and cellulase production. Under both normal and salt-alkali stress conditions, it can significantly promote the photosynthetic capacity of cucumber plants, the growth of both above-ground and underground parts, increase cucumber yield, and improve cucumber quality.

This invention provides a composition comprising milk thistle extract and Akkella myxophilus. This composition can rapidly increase the abundance of Akkella myxophilus in the intestinal environment, promote its proliferation in the intestine, increase the variety and abundance of beneficial intestinal bacteria, colonize beneficial bacteria, and eliminate harmful bacteria. It has the effects of improving intestinal microecology, relieving fatigue, protecting the liver, reducing fat, relieving hangovers, anti-inflammatory, antioxidant, anti-aging, and beautifying the skin.

The application discloses a kind of small molecule slow carbon bacterial fertilizer special for crab culture and a preparation method thereof, and belongs to the technical field of fertilizer preparation and aquaculture.The small molecule slow carbon bacterial fertilizer raw materials include, by weight: 60-70 parts of non-resistant chicken manure, 30-40 parts of corn bran, 5-10 parts of Sophora flavescens seedling, 0.2-0.4 parts of fermentation bacteria, 0.2-0.4 parts of EM bacteria, 0.3-0.5 parts of chitosan, 0.3-0.5 parts of seaweed extract, 4-6 parts of magnesium chloride hexahydrate, and 2-5 parts of diatomite.The preparation method includes raw material pretreatment, fermentation material mixing, fermentation, aging, crushing and screening, nutrient additive and EM bacteria addition, and packaging and storage.The application balances the carbon-nitrogen ratio, nitrogen-phosphorus ratio, calcium-magnesium ratio and silicon nutrients in water, constructs a "water grass-algae-microorganism" microecological balance system, inhibits green moss and blue algae, kills ciliates, proliferates beneficial bacteria, improves crab immunity, realizes water quality control, substrate improvement, water grass maintenance and growth promotion, and is suitable for crab pond mouth whole-cycle breeding, while realizing agricultural waste resource utilization.

The invention provides a composition for improving the intestinal bacterial phase and prolonging the colonization rate of beneficial bacteria in the intestinal tract, the composition comprises Bifidobacterium lactis GKK2, and the composition can be used for improving the intestinal bacterial phase, promoting the colonization rate of the beneficial bacteria in the intestinal tract and prolonging the activity of the beneficial bacteria in the intestinal tract.

The present application relates to the field of probiotics and prebiotics, and in particular to the use of Citrus aurantium, Chichorium intybus, Elsholtzia stauntoni and Hamamelis virginiana as prebiotic mimics (pseudobiotics) that are both medicinal and edible. We first demonstrated that the water extracts of these medicinal and edible herbs significantly improved the constipation symptoms of model mice, improved the pathological damage of the colon of constipated mice, significantly increased the expression of proteins related to intestinal peristalsis and maintenance of the intestinal barrier, promoted the colonization of beneficial bacteria in the intestines of constipated mice, and exhibited effects similar to prebiotics. Therefore, any one of Citrus aurantium, Chichorium intybus, Elsholtzia stauntoni and Hamamelis virginiana or a combination thereof, or a mixture prepared with other substances, can be used to treat constipation in mice, and the mechanism of action may be related to promoting intestinal peristalsis, enhancing the function of the intestinal barrier, and increasing the abundance of beneficial bacteria in the intestines.

The application provides a strain for preventing and treating finger disease of bergamot Bacillus subtilis The application of B702 aims to solve the technical problem that the prevention method of finger disease of bergamot in the prior art is easy to leave residues and pollute the environment. The pathogenic bacteria causing the finger disease of bergamot is identified as Alternaria alternata through tissue separation, pathogenicity determination and morphological and molecular biological (rDNA-ITS and ATPase gene) identification. A bacterial strain with significant antagonistic effect is isolated and screened from the rhizosphere soil of healthy bergamot Bacillus subtilis The B702 is identified as Bacillus subtilis through 16S rRNA gene sequence analysis. The plate confrontation method determination result shows that the Bacillus subtilis strain Bacillus subtilis The average inhibition rate of the B702 to the pathogenic bacteria reaches 92.5% or more, the effect is stable and significant, the strain is a naturally existing plant rhizosphere beneficial bacteria, is safe to human and livestock, does not pollute the environment, provides a new resource for developing green and efficient biological pesticides, and has important significance for realizing the safe production of bergamot.

The application belongs to the technical field of biological medicine, and particularly relates to a traditional Chinese medicine composition and application of intestinal probiotics induced by the traditional Chinese medicine composition in gouty arthritis. The application provides application of intestinal probiotics induced by improved compound Jiawei Ermiaosan in preparation of a medicine for treating gouty arthritis. The improved compound Jiawei Ermiaosan is composed of atractylodes rhizome, Chinese cork tree, coix seed, chingmao grass, Chinese goldthread, dried tangerine or orange peel, mirabilite, sedge, sargentclematis, prepared radix falcaliae and psyllium seed. The composition can regulate the microecological proportion of intestinal flora, eliminate pathogenic bacteria, increase beneficial bacteria, and effectively treat gouty arthritis.

The present invention relates to the field of probiotics, in particular to an Akkermansia muciniphila MG784 for alleviating constipation, promoting defecation, improving intestinal barrier function and intestinal immunity, and its uses. The Akkermansia muciniphila MG784 provided by the present invention has the deposition number DSM 35549. The Akkermansia muciniphila MG784 provided by the present invention can increase the levels of motilin (MTL), gastrin (GAS), and acetylcholine in the gastrointestinal tract, promote intestinal peristalsis, improve the small intestine propulsion rate, and ameliorate defecation time and fecal output. Furthermore, Akkermansia muciniphila MG784 can also alleviate colonic mucosal inflammation, improve the thickness of the digestive tract muscular layer, modulate the structure of the intestinal microbiota, significantly increase the abundance of beneficial bacteria, and decrease the abundance of harmful bacteria.

This invention relates to the application of a traditional Chinese medicine composition in the preparation of a drug for treating intestinal injury. The traditional Chinese medicine composition comprises: 50-150 parts of ginseng, 60-180 parts of polygonatum, 40-90 parts of atractylodes, 30-58 parts of sophora flavescens, 60-180 parts of ophiopogon japonicus, 60-110 parts of rehmannia glutinosa, 40-90 parts of polygonum multiflorum, 60-180 parts of cornus officinalis, 40-90 parts of poria cocos, 35-58 parts of eupatorium fortunei, 35-58 parts of coptis chinensis, 35-90 parts of anemarrhena asphodeloides, 35-58 parts of epimedium, 40-110 parts of salvia miltiorrhiza, 60-180 parts of kudzu root, 80-140 parts of litchi seed, and 40-90 parts of lycium chinense root bark. The above-mentioned traditional Chinese medicine composition can inhibit the expression of TNF, AGE-RAGE, TLR and IL-17 and the infiltration of macrophages. It also helps to regulate the proliferation and differentiation of intestinal cells, restore beneficial bacteria in the intestine, and significantly improve the barrier function of the intestine, thereby effectively improving the damaged intestinal function.

This invention belongs to the field of microbial technology, specifically disclosing a probiotic composition and its application. The probiotic composition provided by this invention includes at least two of the following: prebiotics, vitamin K, and zinc, as well as *Bifidobacterium longum* subsp. infantis i18. The probiotic composition provided by this invention can not only effectively promote the growth and development of premature mice, but also optimize the intestinal flora structure and promote intestinal development in premature infants by reducing the abundance of harmful bacteria and increasing the relative abundance of beneficial bacteria; simultaneously, it can downregulate the levels of pro-inflammatory factors TNF-α and IL-6, reducing intestinal inflammation and lowering the risk of necrotizing enterocolitis in premature infants. This invention can be applied to the preparation of products that improve the intestinal flora of premature infants, promote their growth, or prevent intestinal inflammation.

The application discloses application of forskolin and / or isoforskolin in preparation of a medicine for preventing and / or treating colitis, and belongs to the technical field of medicines.The medicine can significantly improve the disease phenotype of colitis, including relieving weight loss, reducing disease activity index score, improving colon length shortening and histopathological damage.The mechanism of action involves multiple targets: repairing intestinal mucosal barrier function, reducing local inflammation of the colon, and regulating intestinal flora balance, restoring the abundance of beneficial bacteria and inhibiting the proliferation of pathogenic bacteria.The application provides a candidate drug with anti-inflammatory, barrier repair and flora regulation functions for the treatment of colitis, and expands the medical use of forskolin or isoforskolin.

This invention discloses a plant Rossellomorea marisflavi The XJ3-14 strain and its application belong to the field of beneficial bacteria technology. This invention provides... Rossellomorea marisflavi The XJ3-14 strain was isolated for the first time and deposited at the China Center for Type Culture Collection on April 3, 2025, with accession number CCTCC M 2025698. It exhibits good salt and alkali tolerance and can grow well on media containing 16% NaCl or pH 12. It also has the ability to reduce alkali, fix nitrogen, solubilize potassium, dissolve organic phosphorus, dissolve inorganic phosphorus, produce protease and cellulase. Under normal conditions and salt and alkali stress conditions, it can significantly promote the photosynthetic capacity of cucumber plants, the growth of above-ground and underground parts, increase cucumber yield, improve cucumber quality, and can also significantly promote the growth of tomato and other plant seedlings.

The application discloses a construction method of a microbial-metabolite-host regulation network of summer South China calf based on Lactobacillus reuteri, and belongs to the technical field of microorganisms. The construction method comprises the following steps: collecting rumen, fecal sample and whole blood sample of the summer South China calf; obtaining microbial layer data through 16S rRNA sequencing, obtaining metabolite layer data through non-target metabolomics, and obtaining host gene / pathway layer data through whole blood transcriptomics; performing correlation analysis on multi-omics data to obtain a microbial-metabolite-host regulation correlation network. The regulation network significantly improves the growth performance of the summer South China calf, relieves diarrhea and enhances the digestive absorption function by improving the abundance of gastrointestinal beneficial bacteria, promoting the secretion of short-chain fatty acids and activating the host immune antioxidant pathway. The application realizes the precise mechanized application of probiotics by clarifying the regulation correlation among the three, and provides a mechanism-application integrated scheme for healthy breeding of calves.

This invention, entitled "A Composition Containing Pentadecanoic Acid, Parabacterium Species Outer Membrane Vesicles Containing the Composition, and Their Applications," belongs to the field of probiotics and microbial preparations technology. The technical problem this invention aims to solve is that beneficial live bacteria have weak colonization ability in the gut, are easily affected by the gastrointestinal environment, exhibit unstable individual responses, and struggle to simultaneously address immune regulation and barrier repair. This invention provides a composition containing pentadecanoic acid, Parabacterium Species-derived outer membrane vesicles, and their applications. The outer membrane vesicles are derived from *Parabacterium difficile* and are rich in pentadecanoic acid, palmitic acid, and octadecanoic acid. The composition and outer membrane vesicles prepared by this invention can significantly inhibit macrophage M1 polarization and reduce pro-inflammatory cytokines. TNF-α , IL-6 and IL-1 β It can lower the expression level of [something], reduce intracellular reactive oxygen species levels, improve mitochondrial function, significantly alleviate symptoms of colitis induced by sodium dextran sulfate, regulate the intestinal flora structure, and increase the abundance of beneficial bacteria.

This invention relates to the field of antimicrobial peptide technology, specifically to an antimicrobial peptide for maintaining oral flora balance, its preparation method, and its application. The antimicrobial peptide for maintaining oral flora balance according to this invention includes any one of the following polypeptides (a)-(e): (a) a polypeptide whose amino acid sequence includes the amino acid sequence shown in SEQ ID NO. 1; (b) an amino acid sequence composed of amino acid residues shown in SEQ ID NO. 1; (c) a polypeptide formed by substituting and / or deleting and / or adding one or more amino acid residues to the amino acid sequence defined in (a) or (b); (d) a polypeptide having 99% or more, 95% or more, 90% or more, 85% or more, or 80% or more homology to the amino acid sequence defined in (a) or (b); (e) a polypeptide obtained by modifying the N-terminus and / or C-terminus of any one of the polypeptides defined in (a)-(d). The antimicrobial peptides of this invention, used to maintain oral flora balance, exhibit antibacterial and bactericidal activity against oral pathogens while not affecting the normal growth of beneficial bacteria. This balance is maintained, protecting gum health and freshening breath. These antimicrobial peptides are suitable for preparing oral antibacterial agents and related oral care products, regulating the balance of the oral microecology, and thus used to prevent and treat oral diseases, especially dental caries, periodontitis, oral mucosal diseases, and oral cancer. The antimicrobial peptides of this invention are relatively short, making chemical synthesis easier and allowing for direct synthesis into high-purity products. They also exhibit clear effects and have wide applications.

This invention discloses a method for de novo design of antifungal peptides, the obtained peptides, and their applications. The method includes: S1 peptide library retrieval and amino acid preference analysis; S2 peptide design based on multiple parameters; S3 AI high-throughput screening of the peptide library to obtain candidate antifungal peptides. The amino acid sequences of the mentioned antifungal peptides are also disclosed in this invention as shown in SEQ ID Nos: 1 to 3. The antifungal peptides developed by this invention can specifically target and kill fungi such as Malassezia furfur without inhibiting the growth of other beneficial bacteria such as Propionibacterium acnes, thereby effectively regulating the skin surface microecological balance and maintaining skin health; solving the problem that current antimicrobial peptides, due to their broad-spectrum antibacterial properties, can disrupt the skin microecological balance and cause more serious skin diseases.