Composition for regulating micro-ecological balance of skin and application of composition in cosmetics

Abstract

The invention discloses a composition for regulating micro-ecological balance of skin and application of the composition in cosmetics, and the composition is prepared from the following components in percentage by mass: 5-15% of capryloyl hydroximic acid, 0.1-2% of glycerol caprylate and 5-15% of ethylhexylglycerin. The composition can synergistically inhibit harmful bacteria, has a poor inhibition effect on beneficial bacteria, effectively adjusts the proportion of the beneficial bacteria to the harmful bacteria of the skin, further effectively adjusts the micro-ecological balance of the skin, reconstructs the micro-ecological environment of the skin, and is beneficial to the health of the skin.

Description

Technical Field

[0002] The invention belongs to the field of cosmetics, and in particular relates to a composition for regulating skin microecological balance and application thereof in cosmetics. Background Art

[0004] As the largest organ covering the human body, the skin not only shoulders the important task of protecting internal tissues from external damage, but also plays a key role in maintaining the body's water balance and sensing environmental stimuli. In recent years, research in the field of skin microecology has attracted increasing attention from scientists, who have found that the microbial community on the surface of the skin, namely the skin microbiome, has an extremely important impact on skin health. The balance of the skin microbiome is closely related to the health of the skin. Once the microecology is out of balance, it may cause a variety of skin problems, including but not limited to acne, eczema, and sensitive skin.

[0005] In the current skin care and cosmetics market, the use of many products may have an adverse effect on the balance of the skin microbiome. When the skin microbiome is out of balance, its barrier function will be damaged, leading to the growth of harmful bacteria. The overgrowth of these harmful bacteria will induce a series of skin problems, such as excessive skin oil secretion, acne and pimples.

[0006] Strengthening the stability of the resident bacterial flora of the skin and regulating the balance of the bacterial flora can achieve the prevention and treatment of skin problems. However, in the current technical means, there is still a lack of compositions that can effectively regulate the balance of skin microecology. Therefore, it is of great significance to develop a composition that performs well in regulating the balance of skin microecology. Summary of the invention

[0008] In view of the deficiencies in the above-mentioned prior art, the present invention provides a composition for regulating the balance of skin microecology and its application in cosmetics. The composition can synergistically inhibit harmful bacteria, but has a poor inhibitory effect on beneficial bacteria, effectively regulates the ratio of beneficial bacteria and harmful bacteria in the skin, and further effectively regulates the balance of skin microecology, rebuilds the skin microecological environment, and is beneficial to skin health.

[0009] The object of the present invention is to provide a composition for regulating the balance of skin microecology, which comprises, by mass percentage, 5-15% of caprylhydroxamic acid, 0.1-2% of glyceryl caprylate, and 5-15% of ethylhexylglycerol.

[0010] Preferably, the composition for regulating skin microecological balance comprises, by mass percentage, 5.5-14% caprylhydroxamic acid, 0.5-1.5% glyceryl caprylate, and 6.4-14% ethylhexylglycerin.

[0011] Preferably, the composition for regulating the balance of skin microecology comprises, by mass percentage, 10% caprylhydroxamic acid, 1.0% glyceryl caprylate, and 10% ethylhexylglycerin.

[0012] Preferably, the composition for regulating skin microecological balance comprises a balance of solvent.

[0013] Preferably, the solvent is selected from at least one of 1,2-propylene glycol, 1,3-propylene glycol, 1,2-butylene glycol, 1,3-butylene glycol, 1,4-butylene glycol, 1,2-pentanediol, 1,5-pentanediol, isopentyl glycol, isohexylene glycol, 1,2-hexanediol, dipropylene glycol, glycerol and methylpropylene glycol.

[0014] Another object of the present invention is to provide a composition for regulating skin microecological balance and its application in cosmetics.

[0015] Preferably, the composition has the effect of regulating skin microecology.

[0016] Preferably, the cosmetic is selected from any one of a facial mask liquid, a toner, an essence water, and an emulsion.

[0017] Preferably, the amount of the composition is 0.4% to 0.8% of the cosmetic in terms of mass percentage.

[0018] More preferably, the amount of the composition is 0.6% of the cosmetic by mass percentage.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The composition of the present invention can synergistically inhibit harmful bacteria, but has a poor inhibitory effect on beneficial bacteria, effectively regulates the ratio of beneficial bacteria to harmful bacteria in the skin, and further effectively regulates the balance of skin microecology, rebuilds the skin microecological environment, and is beneficial to skin health. DETAILED DESCRIPTION

[0022] In order to enable those skilled in the art to better understand the technical solutions of the present invention, the technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0023] Example 1: Composition of 10% caprylhydroxamic acid, 1.0% glyceryl caprylate, 10% ethylhexylglycerin and 79% 1,2 propylene glycol.

[0024] Example 2: Composition of 5% caprylhydroxamic acid, 2% glyceryl caprylate, 15% ethylhexylglycerin and 78% 1,2 propylene glycol.

[0025] Example 3: Composition of 15% caprylhydroxamic acid, 0.1% glyceryl caprylate, 5% ethylhexylglycerin and 79.9% 1,2 propylene glycol.

[0026] Comparative Example 1: Composition of 21% octanoylhydroxamic acid and 79% 1,2-propylene glycol.

[0027] Comparative Example 2: Composition of 21% glyceryl caprylate and 79% 1,2 propylene glycol.

[0028] Comparative Example 3: Composition of 21% ethylhexylglycerin and 79% 1,2-propylene glycol.

[0029] The preservatives in Table 1 are the compositions of Examples 1 to 3 and Comparative Examples 1 to 3 respectively; the application system is essence water, and the specific formula and production process are shown in Table 1:

[0030] Table 1. Essence water ingredients

[0031]

[0032] 1. Antibacterial effect test

[0033] Refer to WS∕T 650-2019 "Antibacterial and antibacterial effect evaluation method" 5.1.1 Suspension quantitative antibacterial test for antibacterial effect test.

[0034] 1.1 Test materials

[0035] 1.1.1 Test strains

[0036] The bacteria used in this experiment were Staphylococcus aureus ATCC6538, Propionibacterium acnes ATCC11827, Staphylococcus epidermidis ATCC12228, and Staphylococcus hominis subsp. hominis ATCC27844, all of which were obtained from Guangdong Provincial Microbiological Culture Collection Center.

[0037] 1.1.2 Test reagents

[0038] Dilution: 0.03 mol / L phosphate buffer (PBS) (pH 7.2-7.4), culture medium: Staphylococcus aureus, Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus hominis subsp. hominis were cultured on nutrient agar medium.

[0039] 1.1.3 Test equipment

[0040] Constant temperature water bath, timer, Class II biological safety cabinet, etc.

[0041] 1.2 Test steps

[0042] Take the 24h fresh slant culture of the test bacteria, wash it with PBS, and dilute it with PBS to about 5.0×10 5 CFU / mL~4.5×10 6 CFU / mL bacterial suspension is ready for use. Take a sterile test tube, first add 5.0 mL of the essence water samples of Examples 4 to 6 and Comparative Examples 4 to 6, respectively, place it in a 20℃±1℃ water bath for 5 minutes, then add 0.1 mL of the test bacterial suspension, mix quickly and start timing immediately. After the test bacteria interact with the essence water samples of Examples 4 to 6 and Comparative Examples 4 to 6 for the specified test time of 1 hour, respectively aspirate 1.0 mL of the mixture of the test bacteria and the essence water samples of Examples 4 to 6 and Comparative Examples 4 to 6 to inoculate 2 plates, and pour the culture medium. When the amount of bacteria cannot be counted, make a 10-fold serial dilution with PBS, select the appropriate dilution and aspirate 1.0 mL to inoculate 2 plates for live bacterial culture counting. At the same time, use PBS to replace the essence water samples of Examples 4 to 6 and Comparative Examples 4 to 6, respectively, and conduct parallel tests as positive controls. The number of colonies recovered in the positive control is 1.0×10 4 CFU / mL~9.0×10 4 CFU / mL. Take the same batch of PBS and culture medium as negative control. All test samples and control samples are cultured at 36℃±1℃, and the bacterial propagules are cultured for 48h to observe the final results. The test is repeated 3 times and the inhibition rate is calculated.

[0043] 1.3 Calculation of antibacterial rate

[0044]

[0045] Where:

[0046] X—inhibition rate, %;

[0047] A0—the amount of bacteria recovered in the positive control group, in CFU / mL;

[0048] A1—The amount of bacteria recovered in the test group, in CFU / mL.

[0049] 1.4 Result determination

[0050] If the inhibition rate is ≥50%~90%, it is judged to have an antibacterial effect; if the inhibition rate is ≥90%, it is judged to have a strong antibacterial effect.

[0051] The test results are shown in Table 2 below:

[0052] Table 2. Antibacterial effect test results of the essence water of Examples 4 to 6 and Comparative Examples 4 to 6.

[0053] Sample name Testing time Staphylococcus aureus inhibition rate Propionibacterium acnes inhibition rate Staphylococcus epidermidis inhibition rate Staphylococcus aureus subsp. Example 4 1h 99.7% 99.9% 18.3% 35.6% Example 5 1h 92.6% 91.3% 28.9% 29.7% Example 6 1h 96.4% 90.5% 22.5% 33.9% Comparative Example 4 1h 24.3% 18.2% 21.1% 19.3% Comparative Example 5 1h 12.7% 15.9% 15.2% 21.0% Comparative Example 6 1h 14.4% 11.3% 23.4% 24.1%

[0054] It can be clearly seen from the antibacterial effect test results that the essence water of Examples 4 to 6 has a strong inhibitory effect on the skin-harmful bacteria Staphylococcus aureus and Propionibacterium acnes, and the inhibition rates are all greater than 90%, but has almost no inhibitory effect on the skin-beneficial bacteria Staphylococcus epidermidis and Staphylococcus hominis subspecies human, and the inhibition rates are all less than 50%; the essence water of Comparative Examples 4 to 6 has comparable inhibitory effects on the four microorganisms, does not show selectivity, and cannot regulate the microorganisms on the skin; This shows that the compositions of Examples 1 to 3 have obvious selectivity in their inhibitory effects on the skin's beneficial bacteria and harmful bacteria, can regulate the skin's microecology, and are beneficial to skin health.

[0055] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in the relevant field should understand that after reading the specification of this application, the technicians can still modify or replace the specific implementation mode of the present invention with equivalents, but these modifications or changes do not deviate from the scope of protection of the pending claims of the present application.

Claims

1. A composition for regulating skin microecological balance, characterized in that: Calculated by mass percentage, it includes 5-15% caprylhydroxamic acid, 0.1-2% glyceryl caprylate, and 5-15% ethylhexylglycerol.

2. The composition according to claim 1, characterized in that Calculated by mass percentage, it includes 5.5-14% caprylhydroxamic acid, 0.5-1.5% glyceryl caprylate, and 6.4-14% ethylhexylglycerol.

3. The composition according to claim 2, characterized in that Calculated by mass percentage, it includes 10% caprylhydroxamic acid, 1.0% glyceryl caprylate, and 10% ethylhexylglycerin.

4. The composition according to any one of claims 1 to 3, characterized in that The composition also includes a balance of solvent.

5. The composition according to claim 4, characterized in that The solvent is selected from at least one of 1,2-propylene glycol, 1,3-propylene glycol, 1,2-butylene glycol, 1,3-butylene glycol, 1,4-butylene glycol, 1,2-pentanediol, 1,5-pentanediol, isoprene glycol, isohexanediol, 1,2-hexanediol, dipropylene glycol, glycerol, and methylpropylene glycol.

6. Use of the composition for regulating skin microecological balance according to any one of claims 1 to 4 in cosmetics.

7. The use according to claim 6, characterized in that The composition has the effect of regulating skin microecology.

8. The use according to claim 6, characterized in that The cosmetic is selected from any one of a facial mask liquid, a toner, an essence water, and an emulsion.

9. The use according to claim 6, characterized in that In terms of mass percentage, the amount of the composition is 0.4% to 0.8% of the cosmetic.

10. The use according to claim 9, characterized in that In terms of mass percentage, the amount of the composition is 0.6% of the cosmetic.

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