The invention relates to a
gene regulation and control method for improving wheat scab resistance based on an STTM mediated tRF-Lys silencing technology, belongs to the technical field of
gene engineering, and aims to inhibit tRF-Lys through the STTM technology, realize obvious improvement of wheat scab resistance and have multiple innovation values. Firstly, the strategy directly aims at
pathogen infection key targets, the
disease resistance of wheat ears is remarkably improved,
toxin accumulation is effectively reduced, and a new way is provided for guaranteeing the
grain yield and quality. And secondly, endogenous non-coding
RNA is regulated and controlled by a
gene editing means, so that the use of
chemical agents is avoided, the environmental burden is reduced, the ethical dispute of the traditional
transgenic technology is avoided, and the development trend of green
agriculture is met. Besides, the research discloses a new function of tRF-Lys in wheat-gibberellic
disease interaction, enriches a
disease-resistant gene resource
library, and provides a theoretical basis and
technical support for
crop disease-resistant breeding.