The invention discloses a preparation method of a seaweed biological soil conditioner with a saline and alkaline land improvement function. The soil conditioner is prepared with a compound microbial fermentation technology from raw materials including Enteromorpha, kelp and oyster shell powder, has the functions of seaweed fertilizer, microbial fertilizer and organic fertilizer, contains rich seaweed functional active ingredients and large quantities of probiotics, can effectively regulate microbial balance of soil, decompose organic or inorganic constituents such as organic matters, mineral substances and the like in the soil, eliminate soil hardening, desertification, salinization and the like caused by abuse of chemical fertilizer all year round and improve the soil effectively, has the specific function of improving the stress resistance of crops, and improves the crop capacity; the soil conditioner can especially promote release of the organic matters in the soil, improves the physicochemical structure of salinity soil, improves the soil aggregate and permeability, improves the soil fertility, improves the grain yield increase capacity of a saline and alkaline land and is an excellent saline and alkaline land soil conditioner and a crop growth promoter.