461 results about "Selfing" patented technology

The invention discloses a breeding method for a corn male sterile line. A male sterile line of corn selfing line is obtained by backcross breeding with corn S-type cytoplasmic male sterile line MD32CGMCC No.8657 as a donor and the corn selfing line as an acceptor. Experiments demonstrate that the breeding method provided by the invention has the following advantages that (1) by combining a marker-assisted selection technology and the backcross breeding technology, the breeding speed of the sterile line is accelerated and Jing 724 sterile line S-jing 724 can be obtained only by three backcross generations; and (2) the S-type sterile line MD32 selected by the breeding method presents stable and thorough sterility in a plurality of genetic backgrounds and is not used in reported studies on corn hybrid male sterile breeding.

The invention discloses a molecular marker in close linkage with rape crotch angle character QTL (Quantitative Trait Loci) and application. The molecular marker is characterized by carrying out hybridization by taking cabbage type rape variety Shanghai oil 19 as a female parent and Purler as a male parent, establishing F2 segregation population through selfing of hybrid F1, and analyzing, thus obtaining a crotch angle locus qBA1.A06; designing a primer by utilizing an Indel marker at the boundary of the crotch angle locus qBA1.A06 so as to detect the parents and the F2 population, thus obtaining a molecular marker BAIndel76 and a molecular marker BAIdenl79 which are in close linkage with the crotch angle character QTL; identifying a rape gene type formed after hybridization of the two parents by utilizing a marker primer, and carrying out auxiliary selection by utilizing the marker, thus greatly increasing the selection efficiency. According to the molecular marker disclosed by the invention, a novel genetic marker is provided for molecular breeding of the rape plant type, and useful information is also provided for accurate positioning on the crotch angle character QTL of the cabbage type rape and map-based cloning on related genes.

Disclosed is a three-line method for mechanical production of hybrid rice seeds. The method is characterized in that a rice variety with hull color different from normal hull color (abnormal color) is selected to hybridize with a maintainer line rice variety with normal hull color so as to obtain maintainer line rice seeds with abnormal hull color, and trans-breeding is performed to obtain sterile line rice seeds with abnormal hull color; and that the obtained sterile line rice seeds with abnormal hull color and restorer line rice seeds with normal hull color are mechanically sown in a mixed manner, mechanical pollen shedding is carried out during the blooming stage, hybrid rice seeds with abnormal hull color and restorer line rice seeds with normal hull color obtained by rice selfing are mechanically harvested, and hybrid seeds and restorer line seeds are separated by use of a color sorter. The method of the invention can be applied to hybrid rice seed production.

The invention relates to a breeding method for introducing corn parthenogernesis by using amiprofos-methyl, and belongs to the field of planting breeding. The method comprises the following steps of: preparing an inductive agent of which the concentration is 5-200mumol/L from the amiprofos-methyl and water; performing introducing treatment: when filaments sprout to grow to about 2.5mm, spraying or daubing the inductive agent to the filaments, and harvesting to obtain homozygous genotype diploid seeds when seeds are mature; and during seeding in the next season, selecting and identifying by using the conventional method to select head progeny rows according with the breeding aim to obtain a homozygous corn selfing line. The breeding method for introducing the corn parthenogernesis by using the amiprofos-methyl has the positive effects of avoiding the artificial pollination, and being simple in method, easy to operate, low in cost, high in efficiency, and capable of being applied in large scale; in addition, the diploid seeds are directly formed and a chromosome doubling process is not required; and in the breeding method, more than 5 generations are shortened in comparison with a breeding method of the conventional corn selfing line.

The invention discloses a breeding method for amphiprotic fertile autotetraploid crucian carps and an establishment method for strains of the crucian carps. The breeding method includes the following steps that allotetraploid crucian carps are selected from crucian carp hybridization F1 offspring with red crucian carps as female parents and megalobrama amblycephala as male parents, female individuals capable of generating ova of different sizes and male individuals capable of generating watery semen are selected from the allotetraploid crucian carps for hasten parturition, insemination is carried out manually through a dry method, running water incubation is conducted on fertilized ova, then the fertilized ova are bred, and the amphiprotic fertile autotetraploid crucian carps are selected from selfing offspring. Selfing is carried out on the male individuals and the female individuals in the obtained autotetraploid crucian carps, and the strains of the amphiprotic fertile autotetraploid crucian carps can be established and obtained through multi-offspring breeding. Ploid hybridization is conducted on the autotetraploid crucian carp colonies obtained through the breeding method and diploid crucian carps, so allotriploid fishes can be bred on a large scale. The strains of the crucian carps obtained through the breeding method are excellent in character, stable in heredity and high in fertilization rate and hatchability, and the breeding method has a great significance in genetic research of fishes.

The invention belongs to the technical field of rape breeding, and discloses a selection breeding method for a Brassica napus-turnip cabbage cytoplasmic male sterility line (CMS). The method is characterized in that an artificially-bred turnip cabbage allotetraploid as a female parent is hybridized with a Brassica napus variety as a male parent, male sterile plants are isolated from the progeny of hybrid selfing, and a Brassica napus-turnip cabbage cytoplasmic male sterility line with genetic stability is obtained by successive backcross breeding. Restorer line and maintainer line relationship and molecular biological methods are employed to prove genetically and on DNA level respectively that the Brassica napus-turnip cabbage cytoplasmic male sterility line has a sterile cytoplasmic type different from those of Polima cytoplasmic male sterility (pol CMS), turnip cytoplasmic male sterility (ogu CMS, kos CMS) and Brassica juncea cytoplasmic male sterility (hau CMS). The invention adds a new rape cytoplasmic male sterility type for China and the rest of the world.

The invention discloses a continuous recessive character backcrossing technology which is characterized by taking a material which contains no target recessive genes and is ready to be improved as a recurrent parent, and taking a material which contains the target recessive genes as a donor parent; taking the recurrent parent as the parent to be hybridized with the donor parent and harvesting hybrid seeds of the first generation F1 and recurrent parent seeds; and planting the hybrid seeds of the first generation F1 and the recurrent parent seeds till the planting of a group containing backcross hybrid seeds of the second of n generations BCn F2 and till the typing into a target recessive gene isogenic line of the recurrent parent. The invention also discloses a method for creating a purpleleaf marker isogenic line by applying the continuous recessive character backcrossing technology and also a method for transferring new purple leaf marker nucleus sterile line by taking the purple leaf marker isogenic line as an intermediate material. In the methods, a selfing process is combined with a next backcrossing process for the treatment in one season, therefore, the time efficiency is improved by about 50 percent, a small number of dominant-gene-controlled unsuitable characters of existing fine-quality parents can be fast improved, and useful recessive characters can be fast imported to the present high-level heredity background.

The invention discloses a molecular breeding method capable of improving length of cotton fibers by using gossypium barbadense chromosome segment introgression line, which is particularly used for crop (cotton) economical character oriented improvement and new variety bredding. An introgression line IL040-A4-1 of which the fiber length is increased obviously is screened out by cotton fiber detection of the gossypium barbadense chromosome segment introgression line. In the method, introgression line is used as a nonrecurrent parent, Xinluzao No.26, which is a variety promoted in cotton area in north of Xinjiang, is used as a recurrent parent are hybridized, hybridizing, two generations of back crossing and three generations of selfing are performed, molecular markers are also used to help to select cotton new lines with high fiber length. A high-quality and high-yield cotton new line named 'Nannong Haidao No.1' was bred by using the method.

The invention relates to a breeding method for colored-flower rape used for creative agriculture. The method comprises the following steps: selecting a mainly cultivated rape variety with superior growth vigor, medium height, good lodging resistance and disease resistance and large petals as a female parent and a cruciferous plant of varieties with colored petals as a male parent, carrying out hybridization and selecting plants with plant properties similar to those of the female parent rape and flower color properties similar to those of the male parent in segregative generations so as to obtain F0 seeds; sowing the F0 seeds, selecting the main inflorescence of excellent individual plants and reserving seeds thereof to obtain F1 seeds; then carrying out mixed seeding of the F1 seeds; starting with F2 plants, selecting plants with target flower colors in an initial flowering stage, carrying out selfing, bagging and seed reserving, and eliminating individual plants of an original rape flower color variety; and carrying out continuous selfing and separating until in few districts, a variety of a same target flower color appears in the whole district after F5 or F6, and carrying out curtain enclosing, serlfing and seed reserving. With the method provided by the invention, peasants both harvest a product (i.e., rapeseeds) and sell the landscape, and the environment is beautified.

The invention discloses a method for transforming a hot-pepper nucleo-cytoplasmic interactive male-sterile line, a homo-maintainer line and a homo-restorer line, which comprises the following steps of: hybridizing by using a hot-pepper selfing line as a male parent and the hot-pepper nucleo-cytoplasmic interactive male-sterile line as a female parent, simultaneously selfing a male parent plant, selecting sterile plants or other inflorescences in 4-50 corresponding male-parent filial single plants and hot-pepper filial generations for paired test crossing when in filial-generation fertility separation, selfing each male-parent single plant, and respectively processing according to the group fertility expression of test crossing generations to select the male-sterile line, the homo-maintainer line and the homo-restorer line. When in filial-generation group fertility separation, the invention not only can be used for transforming the hot-pepper nucleo-cytoplasmic interactive male-sterile line and the homo-maintainer line from the hot-pepper selfing line, but also can be used for breeding the homo-restorer line, expands the selecting space of the restored male parents of other homologous sterile lines and can be used for screening more excellent three-line combined varieties. A hot-pepper hybrid is produced by adopting a male-sterile three-line technology, all the complex pollination procedures are simplified, and the economic benefit is quite remarkable.

The invention relates to a breeding method for a plant, in particular to a breeding method for broccoli, belonging to the technical field of plant cultivation. The breeding method comprises the following steps of: performing continuous selfing and directional selection for at least five generations through collected broccoli material to obtain a self compatible line of the broccoli to serve as a transformation male parent to be hybridized with a cytoplasmic male sterile material; breeding a cytoplasmic male sterile line with superior comprehensive performance and stable genetic character through backcross transformation of five generations; then hybridizing the cytoplasmic male sterile line with stable genetic character with the superior self compatible line of the broccoli; and performing field repeated identification and screening to obtain superior hybridized combined variety. According to the method, an optimal growth and development environment for the broccoli is created though low-temperature treatment of seeds in coordination with related facilities and equipment; double blossom and seed setting are realized in one year; the breeding progress can be quickened; and the seed quality is improved.

The invention discloses a method for rapidly breeding a sweet-waxy double-recessiveness maize selfing line. Self pollination or intraspecific free powder scattering is conducted on existing sweet-waxy maize varieties, seeds with the phenotype being sunken shrinking are selected from clusters obtained through selfing to be planted, and the stable sweet-waxy double-recessiveness maize selfing line is obtained after continuous selfing. According to the method, the sweet-waxy selfing line does not need to be bred by oneself, adopted materials are simple and easy to obtain, operation is easy and convenient, cost is low, and the breeding term is short. The invention further discloses the method for rapidly breeding the sweet-waxy double-recessiveness maize selfing line. Different sweet-waxy maize varieties are respectively planted according to different sweet genes, the interspecific crossing free powder scattering is conducted on varieties the same in sweet gene, the seeds with the phenotype being sunken shrinking are selected, and the stable sweet-waxy double-recessiveness maize selfing line is obtained after continuous selfing. According to the method, gene polymerization is facilitated, the excellent sweet-waxy double-recessiveness selfing line is easier to select, and a good foundation is laid for widening the maize germplasm resources.

The invention discloses a breeding method of radish with purple peel. According to the method, a male sterile plant of a 'dahongpao' radish is used as a sterile source, a six-generation selfing line c152b of a 'cuanxinhong' radish is used as a transformation material, and a male sterile line c152a is obtained by six-generation backcross. A 'baiyuchun' radish is used as an original material, and a high-generation selfing line s-4-3-2-5-1-6-3 with stable inheritable characters can be obtained by seven-generation single plant selfing. The male sterile line c152a is used as a female parent, and the high-generation selfing line s-4-3-2-5-1-6-3 of 'baiyuchun' is used as a male parent, so that one-generation hybrid variety, named as 'ziyu', of the radish with the purple peel can be obtained by hybrid of the male parent and the female parent. According to the obtained variety 'ziyu', the upper half part of the surface peel of a radish is light purple, the lower half part of the surface peel of the radish is white and white like the color of meat, the root peel of the radish is smooth, the peel of the radish is thin, the radish meat is relatively crisp, the content of water in the radish is relatively high, the radish has the flavors of a white radish and a red radish, the yield is high, and the radish with the purple peel can be suitable for cultivation in autumn and cultivation overwintering or in early spring in greenhouses in the middle and lower reach areas of Yangtze river.

The present invention belongs to the technical field of gene engineering, and specially relates to a new corn haploid inducing line and applications thereof. According to the present invention, transgenic corn expressing a fluorescent protein gene, an insect-resistant gene and anti-weeding-agent gene is adopted as the male parent (male), and is subjected to hybridization with a haploid inducing line female parent (female) to generate a hybridization generation, and multiple back cross, screening and selfing are performed to obtain the new corn haploid inducing line; the problems that the haploid is difficult to identify, the accuracy is not high, the speed is slow, the identifying is dependent on experienced researchers and the like in the previous system are solved with the new corn haploid inducing line of the present invention; the rapid identifying can be performed with the simple device without the experienced researchers, the accuracy is high, the time and the labor are saved, the large-scale haploid production can be achieved, and the new corn haploid inducing line is used for corn breeding; and the new corn haploid inducing line has characteristics of insect resistance and weeding agent resistance, is beneficial for field management and improvement of the quantity and the quality of haploid induction.

The invention provides a five-in-one variety breeding method. The five-in-one variety breeding method comprises the following steps: firstly, taking a hybridized combination A*B or a population variety C as a base material, planting the base material in a strong stress environment at high density and large group, continuously carrying out selfing and test mating by using an IPT ((Isopentenyl-Transferases) method, planting the test mating progenies in regions with remarkably different ecological environments and selecting single plants D of which the test mating progenies meet certain standard; secondly, carrying out DH (Doubled Haploid) breeding by taking the D as the material to obtain more than 1000 haploid seeds, doubling the haploids to obtain more than 100 DH pure lines, carry outing target character selection, genetic background restoration selection and homozygosity identification by using an MAS (Marker Assisted Selection) molecule marker assisted breeding technology and selecting a DH pure line F according with a breeding target; thirdly, taking F as parent and assembling the F with the traditional common pure line cultivar to form a hybridized combination, planting the hybridized combination at more than ten test points in different ecological regions and selecting hybridized combination with strong adaptability, high quality and high yield as a hybrid variety G for breeding. According to the five-in-one variety breeding method provided by the invention, the breeding efficiency is increased.

The invention provides a rapid maize breeding and population improvement method, wherein the method includes the following steps: carrying out genotype identification of a basic population by using ahigh-density SNP molecular marker, combining with field phenotype identification, selecting an excellent individual population, and making the excellent individual population rapidly produce a large number of DH lines by using a double-haploid technology; carrying out multi-point field phenotype identification and combining ability determination on the DH population, selecting individual plants with good performance, carrying out molecular marker identification, and analyzing and mining target trait associated marker loci; establishing a whole-genome selection model; screening a plurality of best DH lines with the established whole-genome selection model, and carrying out mixing pollination to form a new basic population; utilizing the double-haploid technology again to make the basic population rapidly produce a large number of DH lines, carrying out phenotypic prediction screening by the whole-genome selection model, carrying out multi-point field phenotype identification on the screened excellent plants, and screening good selfing lines; and circulating the steps to improve the basic population, and carrying out selected breeding of more good selfing lines.

The invention discloses a culturing method of eurytropic bolting-resisting spring Chinese cabbage free microspores. The culturing method comprises the following steps of: (1) taking a high-generation selfing line material which can easily carry out free microspore cultivation and carrying out hybridization on bolting-resisting spring Chinese cabbage materials of the microspores to be cultured to obtain hybridized seeds; and (2) sowing the hybridized seeds into a nutrition pot and naturally vernalizing in a greenhouse; from the end of March to the last ten days of April of the next year, taking flower buds at a bolting and flowering period to carry out the free microspore cultivation according to a conventional method to obtain a regenerated plant. According to the culturing method disclosed by the invention, a simple hybridization method is adopted to hybridize spring Chinese cabbages with different backgrounds in the market and the selfing line material which can easily carry out the free microspore cultivation, so that a donor plant gene type state is effectively improved, and a donor plant selection range of the cultivation of the bolting-resisting spring Chinese cabbage free microspores is obviously expanded; and the culturing method is beneficial for improving the obtaining possibility of double haploid materials of the spring Chinese cabbages with the different backgrounds, and the breeding efficiency of the spring Chinese cabbages is greatly improved.

The invention discloses a corn selfing line genetic transformation method. The corn selfing line genetic transformation method comprises the following steps that a recombinant expression vector containing target genes is introduced into agrobacteria, and recombined agrobacteria are obtained; young embryos of the corn selfing line are infected with the recombined agrobacteria; the young embryos of the corn selfing line are taken and placed in a co-culture medium for co-culture; the young embryos of the corn selfing line are taken and placed in a recovery medium I, a screening medium I, a screening medium II, a recovery medium II and a differential medium in sequence for culture, and seedlings are obtained; the seedlings are taken and placed in a rooting medium for culture, and corn selfing line transformation seedlings are obtained. When the corn selfing line genetic transformation method is applied to corn selfing line genetic transformation, a high transformation rate can be obtained, and great significance is achieved on research work of corn breeding and the corn gene function.