5 results about "Selfing" patented technology

The invention relates to the technical field of tussah genetic breeding, in particular to a tussah egg color genetic marker assisted breeding method which comprises the following steps: S1, hybridizing: hybridizing a yellow tussah variety as a female parent and a brown tussah variety as a male parent to obtain F1-generation tussah; s2, backcrossing: taking the F1-generation tussah as a male parent and performing backcrossing on the F1-generation tussah and the yellow-egg-color female parent to obtain BC1-generation tussah; s3, selfing: selecting the BC1-generation tussah with yellow egg color for selfing at least one generation to obtain BC1Fn-generation tussah with stable yellow egg color, and completing breeding; wherein n represents the number of selfing generations. The method is simple and efficient, and the novel tussah germplasm can be obtained within 4-5 years.

The application relates to a method for efficiently creating a far-cross hybrid between Chinese cabbage and the genus Eruca, which comprises the following steps: taking the selfing line 'ASTRO' of the genus Eruca as a male parent, wherein the selfing line 'ASTRO' has excellent properties such as drought and flood resistance, disease resistance, salt and alkali tolerance, and the like; taking an excellent Chinese cabbage material '72049' as a female parent; spraying magnesium chloride hexahydrate; and then performing intergeneric hybridization. After embryo rescue and colchicine doubling, a doubled material is obtained, and the doubled material is transplanted into a field. Through cytological identification, morphological observation and pollen fertility identification, the doubled material is finally determined as a true hybrid plant.

The application discloses a melon mutant and variety breeding technology with a leaf stalk and a main stem included at an angle less than 45 degrees, relates to the technical field of melon variety breeding, and comprises the following steps: S1, screening excellent melon germplasm resources with inherent genetic traits of small leaf blades and upright leaf stalks as basic breeding parents, and simultaneously considering comprehensive agronomic traits, disease resistance traits and fruit quality traits of the parents; S2, separating offspring populations by means of artificial hybridization or continuous selfing of the screened breeding parents, and constructing a genetic variation basic population; and S3, under the condition of high-density standardized planting of facilities. The application breeds a new melon variety suitable for high-density cultivation, improves land utilization efficiency, group photosynthetic efficiency and unit area yield, simultaneously improves field ventilation and light penetration conditions, and reduces the risk of disease occurrence. Moreover, due to the fact that the outward expansion degree of the whole plant is obviously reduced, the row width is increased, which is beneficial to mechanical spraying, fertilization and weeding operations, and a large number of labor is reduced.

In contrast to meiosis, where meiotic recombination, random segregation of chromosomes and ploidy reduction occurs, Mitosis instead of Meiosis (MiMe), an engineered form of apomeiosis, leads to unreduced clonal gametes in the Solanaceae (night-shades), and, via selfing, to tetraploid offspring that stably inherit genome-wide heterozygosity and plant phenotypes. In the MiMe system, factors involved in meiotic recombination, sister chromatid cohesion, and meiosis II entry are knocked out or knocked down. Engineered clonal unreduced gamete formation by MiMe can be used as a component of synthetic apomixis, and to develop polyploid genome design.

The invention relates to the technical field of traditional Chinese medicinal material breeding, in particular to a ploidy breeding method and system based on multi-dimensional quality fusion of traditional Chinese medicinal materials, which comprises the following steps: screening out a breeding traditional Chinese medicinal material sample set according to a sample characteristic sequence, performing chromosome ploidy regulation on breeding traditional Chinese medicinal material samples to obtain a polyploidy plant, and pretreating the polyploidy plant to obtain a polyploidy breeding sample set; calculating breeding indexes of the homozygous ploidy plants by utilizing a multi-dimensional evaluation model to obtain a breeding index set; identifying superior plants corresponding to the maximum breeding index; performing environmental adaptability detection on the superior plants to obtain target plants; performing selfing or vegetative propagation on the target plants to obtain offspring plants; and carrying out feature analysis on the offspring plants and removing offspring plants which do not accord with a preset offspring feature standard to obtain target offspring plants. The method can solve the problem of inaccurate evaluation criteria in the breeding process of the traditional Chinese medicinal materials at present.