166 results about "Colchicine" patented technology

The invention relates to colchicine transdermal delivery gel and a preparation method thereof. The colchicine transdermal delivery gel is prepared from the following components in percentage by weight: 0.3 percent to 0.7 percent of colchicine, 70.0 percent to 60.0 percent of a gel matrix, 29.5 percent to 39.5 percent of a dissolution promoter and 0 percent to 10 percent of a penetration enhancer. Wherein the gel matrix is prepared by dissolving a thickening agent in a mixed solvent of water and ethanol, the volume ratio of the water is 70%-10%, and the volume ratio of the ethanol is 30%-90%. According to the colchicine transdermal drug delivery gel provided by the invention, the colchicine transdermal drug delivery gel which is simple in formula and convenient to prepare is provided, and non-toxic and non-irritant materials are used, so that the irritation of colchicine oral preparations and injections is avoided, the toxic and side effects can be reduced, and relatively high drug delivery efficiency can be achieved.

Oral liquid colchicine formulations are described herein. Methods of using the oral liquid colchicine formulations are also provided.

The invention discloses a colchicine external preparation with high intradermal residual quantity and percutaneous transmittance as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. The invention provides a colchicine external preparation with high intradermal residue and transdermal transmittance. The colchicine external preparation comprises: a) colchicine or a pharmaceutically acceptable salt thereof; b) a penetration enhancer; c) pharmaceutically acceptable excipients and / or excipients; the penetration enhancer is at least one of polyglycerol oleate or isosorbide dimethyl ether. The drug loading capacity and the percutaneous transmittance of the colchicine external preparation disclosed by the invention are remarkably improved. When polyglycerol oleate is selected as a penetration enhancer to prepare colchicine gel, the intradermal retention volume can reach 358.08 micrograms within 20 hours. When isosorbide monomethyl ether is selected as a penetration enhancer to prepare ethosome gel, the accumulated penetration amount can reach 106.87 mu g / cm < 2 >, and the intradermal residual amount can reach 90.58 mu g or above.

The invention relates to the technical field of biology, and particularly discloses a sample treatment method for oat karyotype detection, which comprises the following steps: S1, root tip pretreatment: taking root tip tissues after germination of oat seeds, and treating the root tip tissues in an N2O gas environment containing 0.05-0.1% colchicine for 2-3 hours, then transferring into a glacial acetic acid-ethanol mixed solution which is precooled to 4 DEG C and fixing for 15-20 minutes; s2, performing enzymolysis treatment: cutting the fixed root tip tissue to be 1-2mm, soaking the cut root tip tissue into enzymatic hydrolysate, and performing enzymolysis at 35-38 DEG C for 30-40 minutes; according to the method, the treatment time and pressure of the root tip tissue in the N2O gas environment containing 0.05%-0.1% of colchicine are controlled to be 0.5-0.8 MPa, and the treatment temperature is controlled to be 20-25 DEG C. According to the method, stagnation in the middle stage of cell division is effectively promoted, so that the dispersion effect of chromosomes is remarkably improved; the improved stationary liquid not only can effectively fix the chromosome morphology and prevent the chromosome morphology from changing in the subsequent treatment process, but also can significantly inhibit microbial contamination and prolong the preservation time of the sample.

The application provides a method for inducing poly-podophylli rhizoma by using clump buds, comprising the following steps: S1, cutting stem segments from a podophylli rhizoma mother plant, soaking and cleaning to obtain explants; S2, disinfecting and cleaning the explants, inoculating into a clump bud induction medium to obtain podophylli rhizoma clump buds; S3, inoculating the clump buds into doubling liquid I, washing with a liquid clump bud induction medium after culture, and then transferring into doubling liquid II; S4, taking out the clump buds, cleaning, inoculating into a clump bud induction medium, and then inoculating into a bud proliferation medium; S5, waiting until 1-2 cm, screening out potential mutant strains through phenotypes, inoculating into the bud proliferation medium for subculture, and obtaining mutant plants; and S6, rooting culture of the mutant plants, and after root systems grow, planting and raising seedlings, and then poly-podophylli rhizoma plants are obtained. In the research, suitable concentration of colchicine is used for induction treatment twice, and through stage concentration switching and intermittent recovery strategies, the poly-ploid induction efficiency and explant survival are effectively considered.

The invention belongs to the technical field of exosomes, and discloses an exosome preparation method and application, the exosome preparation method comprises the following steps: S1, culturing target cells in an induction medium, and collecting the cells and a culture solution; s2, separating the culture solution to obtain supernate A and cell precipitate; s3, performing chromatographic purification and concentration on the supernate A to obtain the exosome; in the step S1, colchicine is also added in the process of culturing the target cells to synchronize the cell cycle; a polycaprolactone-gelatin composite microcarrier is suspended in the induction culture medium, and nicotinamide, an epidermal growth factor (EGF) and pyruvic acid are contained in the induction culture medium; the method further comprises a step S30 before the step S3, resuspending the cell precipitate obtained in the step S2 with a vesicle protection solution containing trehalose, sucrose and EDTA to obtain a supernatant B, and then combining the supernatant A with the supernatant B. According to the preparation method provided by the invention, the total secretion amount of target cells is large, the intracellular vesicle-exosome precursor can be collected, and the obtained exosome is high in concentration and low in impurity content.

This invention provides a 2-sulfonylpyrimidine-4-amide compound and its uses, belonging to the field of antitumor drug technology. The compound of this invention exhibits excellent antitumor activity targeting the colchicine site of tubulin, demonstrating antiproliferative activity against tumor cells such as HeLa, HepG2, H1299, and MCF-7 at the micromolar level, and significantly inhibiting colony formation in a dose-dependent manner. Furthermore, the compound inhibits tubulin polymerization and disrupts the microtubule network of H1299 cells in vitro, inducing cell cycle arrest in the G2 / M phase and apoptosis. More importantly, the compound can inhibit angiogenesis in HUVECs in vitro. The compound of this invention has great potential in the preparation of microtubule destabilizing agents targeting the colchicine site.

In one aspect, the disclosure relates to a method for treating clonal hematopoiesis of indeterminate potential (CHIP) in a subject, the method including at least the step of administering to the subject a therapeutically effective amount of an inhibitor of receptor activator of nuclear factor κB ligand (RANKL), such as denosumab, a therapeutically effective amount of an anti-inflammatory agent, such as colchicine, or both. In a further aspect, performing the method reduces growth of one or more clones or causes the size of one or more clones to remain constant. In another aspect, each month of performing the method can result in a further reduction of annual clonal growth rate. In a still further aspect, performing the method further treats or prevents a cardiometabolic disease, a cardiovascular disease, or a myeloproliferative disease or disorder. Also disclosed are methods for reducing or maintaining clonal hematopoiesis risk score (CHRS) in a subject.

The present invention relates to a pharmaceutical composition, quasi-drug composition, or cosmetic composition for the prevention, improvement, or treatment of skin diseases, comprising as an active ingredient a tubulin inhibitor and one or more compounds selected from the group consisting of calcium, colchicine, tapinarof, fingolimod, tofacitinib, and pharmaceutically acceptable salts thereof. The combination therapy of the present invention can be used as an active ingredient that simultaneously exhibits activities such as skin moisturizing, whitening, wrinkle improvement, acne relief, skin barrier strengthening, or keratinocyte differentiation, along with the prevention, treatment, or improvement of skin diseases.

Prodrugs of colchicine, de-acetyl colchicine, colcemid, and nocodazole are provided as therapies for the treatment of diseases ameliorated by the inhibition of microtubule polymerization.

The invention discloses a method for creating offspring of buckwheat interspecific hybridization. According to the invention, the immature embryo can be successfully obtained through artificial hybridization by taking wild buckwheat as a female parent and tartary buckwheat as a male parent; in order to solve the problems that the induction rate of an immature embryo on a callus induction culture medium is low and the differentiation rate is low when the callus is subjected to differentiation culture in a differentiation culture medium, the callus induction culture medium and the differentiation culture medium are optimized and screened; the obtained callus induction culture medium and the obtained differentiation culture medium remarkably improve the callus induction rate and the differentiation rate during differentiation culture, regenerated plants are obtained at high frequency, colchicine doubling is performed on the regenerated plants on the basis, and a doubled interspecific hybridization new germplasm is obtained by combining morphological and cytological identification. The method is applied to breeding of high-yield stress-resistant, high-flavone, perennial and other breakthrough new varieties or serves as a hybrid bridge parent, and the excellent characters of stress resistance, high flavone, perennial and the like of wild buckwheat are introduced into buckwheat cultivation.

The present invention relates to a pharmaceutical composition suitable for intraarticular injection comprising an anesthetic and an immediate release or controlled release dosage form comprising colchicine. It also relates to a pharmaceutical composition in powder form comprising an anesthetic and an immediate release or controlled release dosage form comprising colchicine wherein the controlled release dosage form comprising colchicine is in particulate form. It also relates to a pharmaceutical composition in powder form comprising an anesthetic and an immediate release or controlled release dosage form comprising colchicine wherein the controlled release dosage form comprising colchicine is in particulate form, relates to a pharmaceutical composition, to a kit and to the use of said pharmaceutical composition or kit in the treatment of crystal-related and non-crystal-related acute inflammatory arthritis, in particular crystal-related arthropathy or sudden or acute forms of tendinitis and bursitis, by intra-articular injection of said pharmaceutical composition into the joint, and to the use of said pharmaceutical composition or kit in the treatment of crystal-related and non-crystal-related acute inflammatory arthritis, in particular crystal-related arthropathy or sudden or acute forms of tendinitis and bursitis, and to provide pain relief immediately and throughout the treatment.

Provided herein are devices, systems, methods, and compositions, including compositions for coupling to a surface of an implantable article, the compositions comprising an anticoagulant comprising a direct factor Xa inhibitor and at least one of (a) an antiplatelet drug, (b) an anticoagulant comprising a direct factor IIa inhibitor, and (c) an anti-inflammatory drug comprising colchicine.

This invention provides a method for inducing chromosome doubling in rice using ammonia-sulfuron-methyl and its application, belonging to the field of rice breeding technology. In this invention, after culturing callus tissue from rice seeds or embryos, the callus tissue is inoculated into a doubling medium containing 0.005 g / L ammonia-sulfuron-methyl for further culture. Following recovery culture, differentiation culture, and rooting culture, root tips are taken from the plants, and chromosome counting is performed under a microscope. Plants with doubled chromosome numbers are selected as tetraploid rice. Compared to the colchicine-induced chromosome doubling method, this method is more efficient and safer. The successful development of this method provides a new approach for rice breeding and new variety creation.

A preparation method of a colchicine (COL) hydrogel microneedle (MN) is provided, including the following steps: dissolving an acrylamide (AM), N,N′-bis(acryloyl) cysteamine (BACA), and Irgacure 2959 in ultrapure water to obtain a clear gel solution; pouring the clear gel solution into a polydimethylsiloxane (PDMS) mold, conducting low-speed centrifugation, and conducting an ultrasonic treatment to eliminate air bubbles; irradiating the PDMS mold under ultraviolet light, and air-drying in an oven to obtain a hydrogel MN; and adding a COL solution to the hydrogel MN, allowing swelling, air-drying, and demolding. The present disclosure overcomes the shortcoming that cross-linking points are unevenly distributed in the ordinary hydrogel MNs. The hydrogel MN prepared by the present disclosure has a complete needle shape, a neat matrix arrangement, cross-linking points evenly distributed in a network, and excellent mechanical toughness and a superior swelling capacity.

The invention belongs to the field of biological medicine, and discloses a colchicine oral solution and a preparation technology thereof.The colchicine oral solution is prepared from, by mass g / volume ml, 0.005%-0.02% of colchicine, 5%-15% of solvent, 0.85%-1.0% of pH regulator, 0.10%-0.20% of sweetening agent, 0.10%-0.20% of flavoring agent, 0.004%-0.016% of coloring agent and the balance purified water, and the pH of the system is controlled to be 5.7-6.6. According to the invention, by controlling the pH value of the solution system, the stability of the oral solution can be improved under the condition of not using a thickening agent, and a filter membrane can be used for filtering and sterilizing because the thickening agent is not used, so that the use of a bacteriostatic agent is avoided, and the safety of the medicine is improved; in addition, single-dose packaging is adopted, and the problem of microbial contamination of products is thoroughly solved.

The invention discloses a group of biomarkers for predicting preventive response of gout patients to colchicine and application of the biomarkers, and relates to the technical field of biomedicine. The marker provided by the invention comprises Sm d42: 3, Cis-13-docosenic acid (erucic acid), Creatinine (creatinine), Gitoxin (hydroxydigitonin) and Trichosanic acid (tricosic acid). The invention also provides a kit and a detection system which are prepared by applying the marker and are used for predicting the preventive response of the gout patient to colchicine. Through the biomarker, the kit and the prediction system, whether the colchicine is effective in preventing gout attack can be accurately predicted, and more accurate preventive treatment is facilitated.

The invention relates to the technical field of cell biology, and discloses a chromosome aberration test method which comprises the following steps: inoculating CHL cells into a plate with the diameter of 100mm, and culturing in a carbon dioxide incubator at 37 DEG C for 24 hours; 4 hours before the cells are harvested, adding a colchicine solution to block the cells in a mitosis metaphase; adding 0.075 mol / L KCL solution for resuspending the cells, and putting the cells into a water bath of about 37 DEG C for hypotonic for 30 minutes to expand the cells; 5 min before the hypotonic treatment is finished, 1 mL of fixing liquid is added for pre-fixing, after uniform mixing, 200 G centrifugation is conducted for 5-7 min, and supernate is discarded; slowly adding 5mL of fresh stationary liquid, uniformly mixing, fixing in a water bath at about 37 DEG C for 30 minutes, reversing uniformly mixed cells every 10 minutes in the process to fully fix, centrifuging at 300G for 10 minutes, discarding supernate, and fixing twice by the same method; adding a set amount of stationary liquid to resuspend the cells, and fixing overnight at 4 DEG C; the chromosome aberration test method provided by the invention solves the technical problem that the chromosome aberration condition in the genetic toxicology test is not easy to observe in the prior art.

The invention relates to a preparation method and application of colchicine-loaded polydopamine nano-particles, belongs to the technical field of nano-drug delivery, and solves one of the problems of serious sudden drug release, poor targeting property, low bioavailability, large toxic and side effects and the like of an existing colchicine nano-carrier preparation. Comprising the following steps: preparing a macrophage membrane suspension and a platelet membrane suspension for later use; mixing the macrophage membrane suspension, the platelet membrane suspension and the hollow polydopamine nanoparticles to obtain a first mixed solution, and performing ultrasonic treatment on the first mixed solution to obtain a membrane-coated first mixed solution; dispersing the first mixed solution subjected to film coating treatment into a colchicine aqueous solution to obtain a second mixed solution, performing ultrasonic treatment on the second mixed solution, and loading colchicine into hollow polydopamine nanoparticles by utilizing an electrostatic adsorption effect; and at normal temperature, carrying out centrifugal treatment on the second mixed solution subjected to ultrasonic treatment, discarding the supernate, and collecting the precipitate, namely the colchicine-loaded polydopamine nano-particles.

The application discloses application of campion flower flavones in preparation of anti-hepatic fibrosis drugs, and an extraction method of the campion flower flavones, which comprises the following steps: reflux extraction of campion flowers by using ethanol to obtain an extract; recovery of ethanol from the extract under reduced pressure until there is no alcohol smell to obtain campion flower extract; and elution of the campion flower extract through a resin column, and then elution of the resin column by using distilled water and ethanol solution in sequence, recovery of ethanol, and evaporation to dryness to obtain the campion flower flavones. The campion flower flavones have the effect of improving hepatic fibrosis, and with the increase of the drug concentration, the indexes related to liver injury of mice are significantly reduced, and the high-dose campion flower flavones can achieve a similar effect to that of the positive drug colchicine. In addition, the in-vitro experiment shows that the campion flower flavones can induce mitochondrial oxidative stress of hepatic stellate cells, inhibit the activation of the hepatic stellate cells, and reduce collagen deposition in the liver and indexes of serum liver injury, thereby providing a new drug selection for the treatment of hepatic fibrosis.

The invention discloses a method for in-vitro induction of tetraploid grapes, and belongs to the technical field of grape tetraploid induction, and the method comprises the following steps: S1, taking tender diploid grape tissue culture seedlings, removing terminal buds, cutting into stem segments, and pre-culturing for 3-5 days; s2, performing colchicine induction on the pre-cultured stem segments, and inoculating the stem segments into a first culture medium for first culture after the induction is finished; s3, after the first culture is completed, each tissue culture seedling is cut into stem segments, the stem segments are transferred into a second culture medium for second culture, and finally the grape tetraploid tissue culture seedlings are obtained. The invention provides an induction method of tetraploid grapes, which provides a practical basis for polyploid breeding work of grapes. By means of ploidy breeding, the defects that diploid grapes are small in fruit size, large in seed number and poor in resistance are overcome.

The present invention provides a method for inducing Arundo donax polyploids. Through the induction method of the present invention, on the basis of rapid propagation of Arundo donax by tissue culture, the culture medium can be scientifically formulated, the culture conditions can be reasonably adjusted, and Arundo donax polyploids with excellent traits can be obtained quickly and efficiently, effectively reducing the incidence of chimeras. The present invention uses the tillering tissue mass of clustered buds as the induction material, which enriches the existing Arundo donax polyploid induction materials compared with the traditional Arundo donax polyploid induction materials. By using colchicine at a specific concentration to induce the tillering tissue mass, the induction rate of Arundo donax polyploids is increased, the incidence of chimeras is effectively reduced, time and costs are saved, and a new way is provided for the breeding of new Arundo donax varieties.

The invention relates to the technical field of eucommia ulmoides breeding. The invention provides a breeding method and an identification method of triploid eucommia ulmoides, which comprises the following steps: taking eucommia ulmoides branches, cutting, treating with colchicine solution, and culturing to obtain adventitious buds; cultivating the adventitious buds to obtain seedlings, performing ploidy identification on the seedlings, and screening out tetraploid plants; taking the tetraploid plant as a female parent, hybridizing with the diploid male parent, and harvesting triploid eucommia ulmoides seeds. The invention provides the method for efficiently and stably obtaining the homozygous tetraploid eucommia ulmoides clone, the problems of common chimeras and unstable materials in chemical induction are solved, and reliable parents are provided for cross breeding. A set of complete technical system for directionally creating the triploid eucommia ulmoides by taking tetraploid as a female parent and diploid as a male parent (reverse crossing) is established, and the technical blank from creation of a polyploid parent to breeding of a new triploid variety is filled.

The present invention discloses a method for inducing tetraploid olives, belonging to the field of olive breeding. The induction method provided by the present invention comprises treating the growth points between the cotyledons of sterile tissue-cultured olive seedlings with an induction solution, wherein the induction solution is a mixed solution containing 0.1% to 0.5% colchicine and 0.02% to 0.04% oryzalin, prepared using a 0.2% dimethyl sulfoxide (DMSO) aqueous solution as a solvent. The method of the present invention can effectively induce the production of tetraploid olives, while achieving a relatively low material mortality rate and a high material ploidy variation rate.

The invention belongs to the technical field of medical compounds, and relates to preparation of a colchicine binding site inhibitor prodrug and nano-drug with ROS response and application of the colchicine binding site inhibitor prodrug and nano-drug in anti-tumor and anti-inflammatory drugs. The prodrug compound is obtained by coupling a colchicine binding site inhibitor (CBSI) and biotin through a thioketal chain responded by ROS (reactive oxygen species). According to the invention, CBSIs and biotin are connected through ROS-responsive thioketal to synthesize a CBSIs prodrug, and then the CBSIs prodrug is self-assembled through a nano precipitation method to prepare the nano drug of the CBSIs prodrug. The novel nano-drug can obviously improve the water solubility of the CBSIs prodrug. When being applied, the compound can target tumor or inflammation parts and is split by high-concentration ROS in tumor or inflammation tissues, so that CBSIs are released to exert pharmacological activity, and meanwhile, the influence on normal tissues is reduced.

The invention belongs to the technical field of biological medicines, and particularly discloses application of colchicine in preparation of a medicine for treating hypertrophic cardiomyopathy. Researches show that after colchicine is used for treatment, pathological changes such as cardiac fibrosis of hypertrophic cardiomyopathy are relieved. Further experimental results show that after colchicine treatment, the proportion of pro-inflammatory macrophages in the cardiac hypertrophy tissue is reduced, the IL-6 level is reduced, and it is prompted that the anti-inflammatory ability of colchicine can improve the microenvironment of the cardiac hypertrophy tissue, so that fibrosis is relieved, and the cardiac function is improved. The invention proves the clinical transformation potential of colchicine in the treatment of cardiac hypertrophy patients. If the medicine is applied clinically in the future, the risk of sudden cardiac death is expected to be reduced by relieving cardiac fibrosis, and a new treatment choice is provided for improving the prognosis of a hypertrophic cardiomyopathy patient.

The invention discloses application of belly-expanding hippocampus submicron powder in preparation of a composition for preventing and treating hepatic fibrosis. The Ccl4-induced liver fibrosis mouse liver and spleen indexes can be remarkably reduced, AST and ALT levels in serum are reduced, Masson dyeing and Rius Red dyeing observe that collagen fiber deposition of the treated liver tissue is reduced, damage to the liver lobular structure is relieved, and meanwhile the hydroxyproline content in the liver tissue is remarkably reduced. The effect of the invention is equivalent to or superior to that of a positive drug colchicine, the advantage of multi-target intervention is embodied, the safety is high, and a new way is provided for developing a novel traditional Chinese medicine for preventing and treating hepatic fibrosis.

The invention belongs to the technical field of crop variety breeding in modern agriculture, and discloses a method for creating homozygous tetraploid rice by using haploid anther, which makes full use of the characteristic that the number of chromosomes in cells subjected to meiosis is halved, and microspores formed by meiosis are subjected to anther culture at a proper development stage, so that the homozygous tetraploid rice is created. The method comprises the following steps of: a, selecting a diploid rice line with excellent characters as a parent material, and carrying out continuous secondary induction by colchicine to form a callus, and differentiating to emerge seedlings to form a stable homozygous tetraploid rice line; b, selecting anther with microspores in the late uninucleate stage during observation; c, inducing anther calluses; e, differentiating the anther calluses to emerge seedlings; f, seedling callus differentiation and rooting; and g, determining the authenticity of a homozygous tetraploid plant formed by anther culture through morphological and cytological technologies (flow cytometry). The method is simple, low in cost and easy to operate.

The invention provides an improved euchiloglanis kishinouyei chromosome preparation method, which comprises the following steps: gradually raising the temperature and injecting PHA twice: in the euchiloglanis kishinouyei temporary culture process, raising the water temperature from 18 DEG C to 20 DEG C within 12 hours, injecting a first needle of PHA, raising the temperature to 22.5-23 DEG C after 12 hours, injecting a second needle of PHA, and keeping the water temperature at 22.5-23 DEG C unchanged; after the PHA is injected for the second time for 3 hours, colchicine is injected; sampling: washing the head and kidney in normal saline, and collecting cells; 0.5% of KCl is used for hypotonic treatment; fixing with a Carnoy's fixing solution, and centrifuging; dropping by a cold dropping method and naturally drying; and carrying out Giemsa dyeing and flaking. According to the method, the euchiloglanis kishinouyei somatic cell proliferation is promoted through heating and twice PHA injection, so that the metaphase division phase is increased, the form is clear and dispersed, and the karyotype quality and the preparation success rate of the chromosome are remarkably improved.

The present invention relates to a method for saving the national Class I rare and endangered plant, Adiantum spp., from extinction. The method first involves physically treating the Adiantum spp. plants, primarily by uniformly isolating the Adiantum spp. plants that have undergone pre-double treatment under physical space isolation and special environmental condition control. After completing the uniform physical space isolation and special environmental condition control, when the shoots grow to a height of 0.5 cm to 2.5 cm, the shoots of the Adiantum spp. plants are sprayed with a chromosome mutagenesis mixed solvent solution (0.4% to 0.8% amoxifen + 0.01% to 0.05% colchicine + 1 to 3 mg / L CaCl2·2H2O + 0.5 to 1.0 g / L P2O5 + 1.0 g / L to 2 g / L aloe extract), and a control group is set up at the same time. The present invention induces new varieties with both excellent appearance and traits in the shortest possible time, achieving the preservation, creation, and utilization of national Class I rare and endangered plant germplasm resources and protecting biodiversity. At the same time, a new method is provided to avoid the extinction of the germplasm resources of the national first-class rare and endangered plant, the iron wire of lotus leaves.