120 results about "Colchicine" patented technology

The invention discloses a colchicine external preparation with high intradermal residual quantity and percutaneous transmittance as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. The invention provides a colchicine external preparation with high intradermal residue and transdermal transmittance. The colchicine external preparation comprises: a) colchicine or a pharmaceutically acceptable salt thereof; b) a penetration enhancer; c) pharmaceutically acceptable excipients and / or excipients; the penetration enhancer is at least one of polyglycerol oleate or isosorbide dimethyl ether. The drug loading capacity and the percutaneous transmittance of the colchicine external preparation disclosed by the invention are remarkably improved. When polyglycerol oleate is selected as a penetration enhancer to prepare colchicine gel, the intradermal retention volume can reach 358.08 micrograms within 20 hours. When isosorbide monomethyl ether is selected as a penetration enhancer to prepare ethosome gel, the accumulated penetration amount can reach 106.87 mu g / cm < 2 >, and the intradermal residual amount can reach 90.58 mu g or above.

The application provides a method for inducing poly-podophylli rhizoma by using clump buds, comprising the following steps: S1, cutting stem segments from a podophylli rhizoma mother plant, soaking and cleaning to obtain explants; S2, disinfecting and cleaning the explants, inoculating into a clump bud induction medium to obtain podophylli rhizoma clump buds; S3, inoculating the clump buds into doubling liquid I, washing with a liquid clump bud induction medium after culture, and then transferring into doubling liquid II; S4, taking out the clump buds, cleaning, inoculating into a clump bud induction medium, and then inoculating into a bud proliferation medium; S5, waiting until 1-2 cm, screening out potential mutant strains through phenotypes, inoculating into the bud proliferation medium for subculture, and obtaining mutant plants; and S6, rooting culture of the mutant plants, and after root systems grow, planting and raising seedlings, and then poly-podophylli rhizoma plants are obtained. In the research, suitable concentration of colchicine is used for induction treatment twice, and through stage concentration switching and intermittent recovery strategies, the poly-ploid induction efficiency and explant survival are effectively considered.

The invention belongs to the technical field of exosomes, and discloses an exosome preparation method and application, the exosome preparation method comprises the following steps: S1, culturing target cells in an induction medium, and collecting the cells and a culture solution; s2, separating the culture solution to obtain supernate A and cell precipitate; s3, performing chromatographic purification and concentration on the supernate A to obtain the exosome; in the step S1, colchicine is also added in the process of culturing the target cells to synchronize the cell cycle; a polycaprolactone-gelatin composite microcarrier is suspended in the induction culture medium, and nicotinamide, an epidermal growth factor (EGF) and pyruvic acid are contained in the induction culture medium; the method further comprises a step S30 before the step S3, resuspending the cell precipitate obtained in the step S2 with a vesicle protection solution containing trehalose, sucrose and EDTA to obtain a supernatant B, and then combining the supernatant A with the supernatant B. According to the preparation method provided by the invention, the total secretion amount of target cells is large, the intracellular vesicle-exosome precursor can be collected, and the obtained exosome is high in concentration and low in impurity content.

This invention provides a 2-sulfonylpyrimidine-4-amide compound and its uses, belonging to the field of antitumor drug technology. The compound of this invention exhibits excellent antitumor activity targeting the colchicine site of tubulin, demonstrating antiproliferative activity against tumor cells such as HeLa, HepG2, H1299, and MCF-7 at the micromolar level, and significantly inhibiting colony formation in a dose-dependent manner. Furthermore, the compound inhibits tubulin polymerization and disrupts the microtubule network of H1299 cells in vitro, inducing cell cycle arrest in the G2 / M phase and apoptosis. More importantly, the compound can inhibit angiogenesis in HUVECs in vitro. The compound of this invention has great potential in the preparation of microtubule destabilizing agents targeting the colchicine site.

In one aspect, the disclosure relates to a method for treating clonal hematopoiesis of indeterminate potential (CHIP) in a subject, the method including at least the step of administering to the subject a therapeutically effective amount of an inhibitor of receptor activator of nuclear factor κB ligand (RANKL), such as denosumab, a therapeutically effective amount of an anti-inflammatory agent, such as colchicine, or both. In a further aspect, performing the method reduces growth of one or more clones or causes the size of one or more clones to remain constant. In another aspect, each month of performing the method can result in a further reduction of annual clonal growth rate. In a still further aspect, performing the method further treats or prevents a cardiometabolic disease, a cardiovascular disease, or a myeloproliferative disease or disorder. Also disclosed are methods for reducing or maintaining clonal hematopoiesis risk score (CHRS) in a subject.

The present invention relates to a pharmaceutical composition, quasi-drug composition, or cosmetic composition for the prevention, improvement, or treatment of skin diseases, comprising as an active ingredient a tubulin inhibitor and one or more compounds selected from the group consisting of calcium, colchicine, tapinarof, fingolimod, tofacitinib, and pharmaceutically acceptable salts thereof. The combination therapy of the present invention can be used as an active ingredient that simultaneously exhibits activities such as skin moisturizing, whitening, wrinkle improvement, acne relief, skin barrier strengthening, or keratinocyte differentiation, along with the prevention, treatment, or improvement of skin diseases.

Prodrugs of colchicine, de-acetyl colchicine, colcemid, and nocodazole are provided as therapies for the treatment of diseases ameliorated by the inhibition of microtubule polymerization.

The invention discloses a method for creating offspring of buckwheat interspecific hybridization. According to the invention, the immature embryo can be successfully obtained through artificial hybridization by taking wild buckwheat as a female parent and tartary buckwheat as a male parent; in order to solve the problems that the induction rate of an immature embryo on a callus induction culture medium is low and the differentiation rate is low when the callus is subjected to differentiation culture in a differentiation culture medium, the callus induction culture medium and the differentiation culture medium are optimized and screened; the obtained callus induction culture medium and the obtained differentiation culture medium remarkably improve the callus induction rate and the differentiation rate during differentiation culture, regenerated plants are obtained at high frequency, colchicine doubling is performed on the regenerated plants on the basis, and a doubled interspecific hybridization new germplasm is obtained by combining morphological and cytological identification. The method is applied to breeding of high-yield stress-resistant, high-flavone, perennial and other breakthrough new varieties or serves as a hybrid bridge parent, and the excellent characters of stress resistance, high flavone, perennial and the like of wild buckwheat are introduced into buckwheat cultivation.

The present invention relates to a pharmaceutical composition suitable for intraarticular injection comprising an anesthetic and an immediate release or controlled release dosage form comprising colchicine. It also relates to a pharmaceutical composition in powder form comprising an anesthetic and an immediate release or controlled release dosage form comprising colchicine wherein the controlled release dosage form comprising colchicine is in particulate form. It also relates to a pharmaceutical composition in powder form comprising an anesthetic and an immediate release or controlled release dosage form comprising colchicine wherein the controlled release dosage form comprising colchicine is in particulate form, relates to a pharmaceutical composition, to a kit and to the use of said pharmaceutical composition or kit in the treatment of crystal-related and non-crystal-related acute inflammatory arthritis, in particular crystal-related arthropathy or sudden or acute forms of tendinitis and bursitis, by intra-articular injection of said pharmaceutical composition into the joint, and to the use of said pharmaceutical composition or kit in the treatment of crystal-related and non-crystal-related acute inflammatory arthritis, in particular crystal-related arthropathy or sudden or acute forms of tendinitis and bursitis, and to provide pain relief immediately and throughout the treatment.

Provided herein are devices, systems, methods, and compositions, including compositions for coupling to a surface of an implantable article, the compositions comprising an anticoagulant comprising a direct factor Xa inhibitor and at least one of (a) an antiplatelet drug, (b) an anticoagulant comprising a direct factor IIa inhibitor, and (c) an anti-inflammatory drug comprising colchicine.

This invention provides a method for inducing chromosome doubling in rice using ammonia-sulfuron-methyl and its application, belonging to the field of rice breeding technology. In this invention, after culturing callus tissue from rice seeds or embryos, the callus tissue is inoculated into a doubling medium containing 0.005 g / L ammonia-sulfuron-methyl for further culture. Following recovery culture, differentiation culture, and rooting culture, root tips are taken from the plants, and chromosome counting is performed under a microscope. Plants with doubled chromosome numbers are selected as tetraploid rice. Compared to the colchicine-induced chromosome doubling method, this method is more efficient and safer. The successful development of this method provides a new approach for rice breeding and new variety creation.

The invention belongs to the field of biological medicine, and discloses a colchicine oral solution and a preparation technology thereof.The colchicine oral solution is prepared from, by mass g / volume ml, 0.005%-0.02% of colchicine, 5%-15% of solvent, 0.85%-1.0% of pH regulator, 0.10%-0.20% of sweetening agent, 0.10%-0.20% of flavoring agent, 0.004%-0.016% of coloring agent and the balance purified water, and the pH of the system is controlled to be 5.7-6.6. According to the invention, by controlling the pH value of the solution system, the stability of the oral solution can be improved under the condition of not using a thickening agent, and a filter membrane can be used for filtering and sterilizing because the thickening agent is not used, so that the use of a bacteriostatic agent is avoided, and the safety of the medicine is improved; in addition, single-dose packaging is adopted, and the problem of microbial contamination of products is thoroughly solved.

The invention relates to the technical field of cell biology, and discloses a chromosome aberration test method which comprises the following steps: inoculating CHL cells into a plate with the diameter of 100mm, and culturing in a carbon dioxide incubator at 37 DEG C for 24 hours; 4 hours before the cells are harvested, adding a colchicine solution to block the cells in a mitosis metaphase; adding 0.075 mol / L KCL solution for resuspending the cells, and putting the cells into a water bath of about 37 DEG C for hypotonic for 30 minutes to expand the cells; 5 min before the hypotonic treatment is finished, 1 mL of fixing liquid is added for pre-fixing, after uniform mixing, 200 G centrifugation is conducted for 5-7 min, and supernate is discarded; slowly adding 5mL of fresh stationary liquid, uniformly mixing, fixing in a water bath at about 37 DEG C for 30 minutes, reversing uniformly mixed cells every 10 minutes in the process to fully fix, centrifuging at 300G for 10 minutes, discarding supernate, and fixing twice by the same method; adding a set amount of stationary liquid to resuspend the cells, and fixing overnight at 4 DEG C; the chromosome aberration test method provided by the invention solves the technical problem that the chromosome aberration condition in the genetic toxicology test is not easy to observe in the prior art.

The invention relates to a preparation method and application of colchicine-loaded polydopamine nano-particles, belongs to the technical field of nano-drug delivery, and solves one of the problems of serious sudden drug release, poor targeting property, low bioavailability, large toxic and side effects and the like of an existing colchicine nano-carrier preparation. Comprising the following steps: preparing a macrophage membrane suspension and a platelet membrane suspension for later use; mixing the macrophage membrane suspension, the platelet membrane suspension and the hollow polydopamine nanoparticles to obtain a first mixed solution, and performing ultrasonic treatment on the first mixed solution to obtain a membrane-coated first mixed solution; dispersing the first mixed solution subjected to film coating treatment into a colchicine aqueous solution to obtain a second mixed solution, performing ultrasonic treatment on the second mixed solution, and loading colchicine into hollow polydopamine nanoparticles by utilizing an electrostatic adsorption effect; and at normal temperature, carrying out centrifugal treatment on the second mixed solution subjected to ultrasonic treatment, discarding the supernate, and collecting the precipitate, namely the colchicine-loaded polydopamine nano-particles.

The application discloses application of campion flower flavones in preparation of anti-hepatic fibrosis drugs, and an extraction method of the campion flower flavones, which comprises the following steps: reflux extraction of campion flowers by using ethanol to obtain an extract; recovery of ethanol from the extract under reduced pressure until there is no alcohol smell to obtain campion flower extract; and elution of the campion flower extract through a resin column, and then elution of the resin column by using distilled water and ethanol solution in sequence, recovery of ethanol, and evaporation to dryness to obtain the campion flower flavones. The campion flower flavones have the effect of improving hepatic fibrosis, and with the increase of the drug concentration, the indexes related to liver injury of mice are significantly reduced, and the high-dose campion flower flavones can achieve a similar effect to that of the positive drug colchicine. In addition, the in-vitro experiment shows that the campion flower flavones can induce mitochondrial oxidative stress of hepatic stellate cells, inhibit the activation of the hepatic stellate cells, and reduce collagen deposition in the liver and indexes of serum liver injury, thereby providing a new drug selection for the treatment of hepatic fibrosis.

The invention relates to the technical field of eucommia ulmoides breeding. The invention provides a breeding method and an identification method of triploid eucommia ulmoides, which comprises the following steps: taking eucommia ulmoides branches, cutting, treating with colchicine solution, and culturing to obtain adventitious buds; cultivating the adventitious buds to obtain seedlings, performing ploidy identification on the seedlings, and screening out tetraploid plants; taking the tetraploid plant as a female parent, hybridizing with the diploid male parent, and harvesting triploid eucommia ulmoides seeds. The invention provides the method for efficiently and stably obtaining the homozygous tetraploid eucommia ulmoides clone, the problems of common chimeras and unstable materials in chemical induction are solved, and reliable parents are provided for cross breeding. A set of complete technical system for directionally creating the triploid eucommia ulmoides by taking tetraploid as a female parent and diploid as a male parent (reverse crossing) is established, and the technical blank from creation of a polyploid parent to breeding of a new triploid variety is filled.

The present invention discloses a method for inducing tetraploid olives, belonging to the field of olive breeding. The induction method provided by the present invention comprises treating the growth points between the cotyledons of sterile tissue-cultured olive seedlings with an induction solution, wherein the induction solution is a mixed solution containing 0.1% to 0.5% colchicine and 0.02% to 0.04% oryzalin, prepared using a 0.2% dimethyl sulfoxide (DMSO) aqueous solution as a solvent. The method of the present invention can effectively induce the production of tetraploid olives, while achieving a relatively low material mortality rate and a high material ploidy variation rate.

The invention belongs to the technical field of biological medicines, and particularly discloses application of colchicine in preparation of a medicine for treating hypertrophic cardiomyopathy. Researches show that after colchicine is used for treatment, pathological changes such as cardiac fibrosis of hypertrophic cardiomyopathy are relieved. Further experimental results show that after colchicine treatment, the proportion of pro-inflammatory macrophages in the cardiac hypertrophy tissue is reduced, the IL-6 level is reduced, and it is prompted that the anti-inflammatory ability of colchicine can improve the microenvironment of the cardiac hypertrophy tissue, so that fibrosis is relieved, and the cardiac function is improved. The invention proves the clinical transformation potential of colchicine in the treatment of cardiac hypertrophy patients. If the medicine is applied clinically in the future, the risk of sudden cardiac death is expected to be reduced by relieving cardiac fibrosis, and a new treatment choice is provided for improving the prognosis of a hypertrophic cardiomyopathy patient.

The invention discloses application of belly-expanding hippocampus submicron powder in preparation of a composition for preventing and treating hepatic fibrosis. The Ccl4-induced liver fibrosis mouse liver and spleen indexes can be remarkably reduced, AST and ALT levels in serum are reduced, Masson dyeing and Rius Red dyeing observe that collagen fiber deposition of the treated liver tissue is reduced, damage to the liver lobular structure is relieved, and meanwhile the hydroxyproline content in the liver tissue is remarkably reduced. The effect of the invention is equivalent to or superior to that of a positive drug colchicine, the advantage of multi-target intervention is embodied, the safety is high, and a new way is provided for developing a novel traditional Chinese medicine for preventing and treating hepatic fibrosis.

The invention provides an improved euchiloglanis kishinouyei chromosome preparation method, which comprises the following steps: gradually raising the temperature and injecting PHA twice: in the euchiloglanis kishinouyei temporary culture process, raising the water temperature from 18 DEG C to 20 DEG C within 12 hours, injecting a first needle of PHA, raising the temperature to 22.5-23 DEG C after 12 hours, injecting a second needle of PHA, and keeping the water temperature at 22.5-23 DEG C unchanged; after the PHA is injected for the second time for 3 hours, colchicine is injected; sampling: washing the head and kidney in normal saline, and collecting cells; 0.5% of KCl is used for hypotonic treatment; fixing with a Carnoy's fixing solution, and centrifuging; dropping by a cold dropping method and naturally drying; and carrying out Giemsa dyeing and flaking. According to the method, the euchiloglanis kishinouyei somatic cell proliferation is promoted through heating and twice PHA injection, so that the metaphase division phase is increased, the form is clear and dispersed, and the karyotype quality and the preparation success rate of the chromosome are remarkably improved.

The present invention relates to a method for saving the national Class I rare and endangered plant, Adiantum spp., from extinction. The method first involves physically treating the Adiantum spp. plants, primarily by uniformly isolating the Adiantum spp. plants that have undergone pre-double treatment under physical space isolation and special environmental condition control. After completing the uniform physical space isolation and special environmental condition control, when the shoots grow to a height of 0.5 cm to 2.5 cm, the shoots of the Adiantum spp. plants are sprayed with a chromosome mutagenesis mixed solvent solution (0.4% to 0.8% amoxifen + 0.01% to 0.05% colchicine + 1 to 3 mg / L CaCl2·2H2O + 0.5 to 1.0 g / L P2O5 + 1.0 g / L to 2 g / L aloe extract), and a control group is set up at the same time. The present invention induces new varieties with both excellent appearance and traits in the shortest possible time, achieving the preservation, creation, and utilization of national Class I rare and endangered plant germplasm resources and protecting biodiversity. At the same time, a new method is provided to avoid the extinction of the germplasm resources of the national first-class rare and endangered plant, the iron wire of lotus leaves.

The invention belongs to the technical field of pharmaceutical preparations, and particularly discloses a colchicine external nano latex agent as well as a preparation method and application thereof.The colchicine external nano latex agent is prepared from the following raw materials in percentage by mass: 0.1%-1.2% of colchicine, 0.5%-25% of an oil phase matrix, 2%-20% of a surfactant, 2%-15% of a cosurfactant, 0%-15% of a transdermal absorption enhancer, 3%-3.5% of a gel matrix, 0%-0.5% of a pH regulator and the balance of water, totaling 100%. According to the colchicine external nano latex agent, the optimal prescription is obtained by applying a pseudo-ternary phase diagram and combining with D-optimal mix design, the particle size of the nano emulsion is stably controlled to be about 14 nm, and the small particle size means faster Brownian movement and stronger penetration of skin cuticle gaps or improvement of the infiltration capacity through appendages such as hair follicles, so that the colchicine external nano latex agent has a good application prospect. The skin penetration efficiency is higher than that of other prescriptions with larger particle sizes, and the nanoemulsion with small particle size hardly shows lag time.

Pharmaceutical compositions of colchicine for once-a-day oral administration are provided. The formulations comprise a sustained-release component and an optional immediate-release component, the compositions of which can be selectively adjusted, respectively, to release the active ingredient along a pre-determined or desired release profile. Methods of treating or preventing cardiovascular disease and / or inflammatory disease in mammalian subjects comprising the administration of the novel formulations disclosed herein are also provided.

The invention relates to the technical field of traditional Chinese medicine propagation and quality improvement, and discloses a homeostatic pseudobulb-based bletilla striata autotetraploid induction and purification method, according to the research, a homeostatic subculture pseudobulb is treated by colchicine, an autotetraploid (2n = 4x = 64) is successfully induced, and the induction rate reaches 26.65%. A bud eye purification strategy is innovatively adopted, so that the chimera rate is reduced to 3.35%, and the ploidy stability is verified through flow cytometry and chromosome counting. Tetraploid plants show typical'canalization 'characteristics, and the pseudobulb multiplication coefficient is obviously higher than that of diploid plants. The established pseudobulb induction-bud eye purification-ploidy stabilization system provides an efficient normal form for polyploidy breeding of endangered medicinal orchid plants, and the tetraploid material has the characteristics of growth vigor enhancement and sustainable propagation and has great industrialization potential.

The present application relates to a transdermal patch containing colchicine and a preparation method and use thereof. The transdermal patch comprises a matrix layer, wherein the matrix layer comprises an active ingredient colchicine, a hot melt pressure sensitive adhesive and optionally other pharmaceutically acceptable adjuvants, wherein the content of the colchicine is 0.1% to 5%, the content of the hot melt pressure sensitive adhesive is 1.9% to 99.9%, and the content of the other pharmaceutically acceptable adjuvants is 0% to 98% based on the total weight of the matrix layer. The transdermal patch containing colchicine of the present application has no local irritation during and after application, no gastrointestinal side effects, high patient compliance, strong drug permeability, good therapeutic effect, and can be used for preventing and / or treating gout and / or pericarditis.

The invention belongs to the technical field of pharmaceutical preparations, particularly discloses a colchicine and lidocaine eutectic mixture as well as a preparation method and application thereof, and aims to solve the problems that colchicine is poor in transdermal effect, large in gastrointestinal tract side effect after oral administration and insufficient to meet the anti-inflammatory and analgesic requirements of acute gout in time. According to the invention, colchicine and lidocaine are mixed according to a molar ratio of 0.1: 0.9-0.9: 0.1 to prepare a eutectic mixture with a melting point of 46.1-64.7 DEG C through a solvent-assisted grinding method, and the eutectic mixture is also prepared into an external pharmaceutical composition. The solubility and transdermal effect of the mixture are remarkably improved, colchicine and lidocaine can be locally and efficiently delivered to lesion joints, the complementary and synergistic effects of the two drugs on anti-inflammatory and analgesic mechanisms are fully played, meanwhile, the gastrointestinal toxic and side effects of oral administration are avoided, the safety is good, the skin tolerance is good, and the application prospect is wide. The medicine is suitable for treating acute gouty arthritis.

This invention discloses a uric acid-lowering drug composition, which comprises one or more of the following: levocarnitine, levocarnitine derivatives, pharmaceutically acceptable salts of levocarnitine or its derivatives, glucocorticoids, colchicine, nonsteroidal anti-inflammatory drugs, IL-1β inhibitors, drugs that inhibit uric acid production, drugs that promote uric acid excretion, and uricase. The composition has the effects of lowering uric acid, protecting liver and kidney function, and inhibiting leukopenia.

The invention discloses a colchicine tablet with high stability, which comprises colchicine, a filler, an adhesive, a disintegrating agent, an antioxidant, an opacifying agent, a flow aid and a lubricant, and the antioxidant is alpha-tocopherol; the invention also provides a preparation method of the colchicine tablet. The colchicine and the antioxidant alpha-tocopherol are dissolved in ethanol and then are granulated, so that the colchicine is prevented from being decomposed in water, the increase of related substances can be effectively reduced, and the stability of the colchicine tablet is improved. The compressibility and hardness of the colchicine tablet can be obviously improved by adding the microcrystalline cellulose into the prescription. In addition, a small amount of opacifying agent titanium dioxide is added into the prescription, so that the storage stability of the colchicine tablet can be obviously improved.

The present application relates to the technical field of plant breeding, and discloses a method for efficiently inducing peony subterranean bud polyploidy and application thereof, comprising subterranean bud disinfection, cell synchronization, minimally invasive pretreatment, preparation of CS- Alg / PPy photothermal response slow-release microspheres, loading of composite inducer, microsphere implantation and photothermal regulation induction, polyploidy identification screening and transplanting, wherein the composite inducer comprises colchicine, 6-BA and graphene quantum dots, can block chromosome separation, realize cell chromosome doubling, regulate growth point cell division, simultaneously repair cell damage, improve bud survival rate, assist photothermal regulation to realize precise drug release, and promote expression of polyploid plant excellent traits. The method has high induction rate, high homozygosity and high bud survival rate, is suitable for cultivation of ornamental, cut flower, medicinal and stress-resistant peonies, and is advanced and practical in technology.

The invention provides a microtubule inhibitor with antiviral activity and application of the microtubule inhibitor, and belongs to the field of biological medicine. The microtubule inhibitor is a compound as shown in a formula I, a salt or a stereoisomer thereof. A microtubule inhibitor small molecule compound of a colchicine binding site is found through research, and the microtubule inhibitor small molecule compound has an excellent inhibition effect on microtubule polymerization and is even superior to colchicine. It is found through research that the compound has excellent antiviral activity on various coronaviruses, and especially the compound W66 has excellent antiviral activity. The invention provides more candidate compounds for antiviral microtubule inhibitors, and has a good application prospect. Formula I.