Method for doubling corn haploid by herbicide and special herbicide of method

Abstract

The invention discloses a method for doubling corn haploid by a herbicide and the special herbicide of the method. According to the method for doubling the corn haploid, the corn haploid is processed by the special herbicide to obtain corn diploid; and an active ingredient of the special herbicide is any one of the following chemical compounds: amiprophos-methl, propyzamide and trifluralin. The special herbicide consists of the active ingredient of the special herbicide, accessory ingredients and water; and the accessory ingredients consist of dimethyl sulfoxide and TWEEN-80 or dimethyl sulfoxide, TWEEN-80 and thienone. The special herbicide provided by the invention causes small environment pollution and is low in toxicity and residue and prone to degradation, and the dead rate of seedlings is 0 after the corn haploid is processed by a center dropping method with the special herbicide. The method is simple, convenient and efficient, and the doubling rate can reach 27.9%; and the doubling rate of the corn haploid processed by a bud immersion method with the special herbicide can reach 17.9%, and the dead rate of seedlings is obviously lower than that of the seedlings processed by colchicine. The method and the herbicide are suitable for large-scale operation, and can be applicable to large-scale breeding of the corn haploid.

Description

technical field [0001] The invention relates to a method for doubling corn haploid by using herbicide and special herbicide. Background technique [0002] In recent years, the large-scale application of maize haploid breeding technology based on induced lines has become one of the three core technologies of modern maize breeding comparable to molecular marker-assisted breeding technology and transgenic technology. Maize is the only crop that uses genetic methods to breed haploids on a large scale, but its natural and chemical doubling rates are still low, resulting in the loss of many haploid materials with excellent agronomic and yield traits due to failure to double, resulting in large Loss. [0003] At present, colchicine is mainly used for chemical doubling of corn haploids. This method has many disadvantages: it is easy to form chimeras during processing; it leads to abnormal growth, dead seedlings, and deformities of plants; the processing method is cumbersome and is ...

AI Technical Summary

Problems solved by technology

Maize is the only crop that uses genetic methods to breed haploids on a large scale, but its natural and chemical doubling rates are still low, resulting in the loss of many haploid materials with excellent agronomic and yield traits due to failure to double, resulting in large Loss

[0003] At present, colchicine is mainly used for chemical doubling of corn haploids. This method has many disadvantages: it is easy to form chimeras during processing; it leads to abnormal growth, dead seedlings, and deformities of plants; the processing method is cumbersome and is not suitable for large-scale applications; Known as highly toxic, easy to cause pollution to the environment

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