Method for doubling corn haploid by herbicide and special herbicide of method

A herbicide, haploid technology, applied in herbicides and algicides, botanical equipment and methods, chemicals for biological control, etc., can solve problems such as loss, dead seedlings, deformities, etc. , the effect of the simple method

Active Publication Date: 2013-04-24
CHINA AGRI UNIV
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Problems solved by technology

Maize is the only crop that uses genetic methods to breed haploids on a large scale, but its natural and chemical doubling rates are still low, resulting in the loss of many haploid materials with excellent agronomic and yield traits due to failure to double, resulting in large Loss
[0003] At present, colchicine is mainly used for chemi

Method used

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  • Method for doubling corn haploid by herbicide and special herbicide of method
  • Method for doubling corn haploid by herbicide and special herbicide of method
  • Method for doubling corn haploid by herbicide and special herbicide of method

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Effect test

Embodiment 1

[0032] Embodiment 1, utilize special-purpose herbicide to double the corn haploid

[0033] (1) Obtaining Haploid Grain of Maize

[0034] The maize haploid induction line Stock6 (Garst Seed Co, USA) was used as the male parent, and Xianyu 335 (Dunhuang Seed Industry Pioneer Fine Breed Co., Ltd.) was used as the female parent for hybridization ( figure 1 ). After the seeds are mature and dried, the seeds of the purple top and white embryo are selected and set aside.

[0035] (2) Identification of maize haploid

[0036] 1. Identification of genetic markers

[0037] Based on the Navajo genetic marker system, the purple top and purple embryo of the grain are the dominant markers. The harvested hybrid seeds were identified one by one. The seeds of the purple-topped and white embryos were haploid, the seeds of the purple-topped purple embryos were diploid in normal hybridization, and the white-topped and white embryos were diploid seeds polluted by pollen. There are few embryole...

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Abstract

The invention discloses a method for doubling corn haploid by a herbicide and the special herbicide of the method. According to the method for doubling the corn haploid, the corn haploid is processed by the special herbicide to obtain corn diploid; and an active ingredient of the special herbicide is any one of the following chemical compounds: amiprophos-methl, propyzamide and trifluralin. The special herbicide consists of the active ingredient of the special herbicide, accessory ingredients and water; and the accessory ingredients consist of dimethyl sulfoxide and TWEEN-80 or dimethyl sulfoxide, TWEEN-80 and thienone. The special herbicide provided by the invention causes small environment pollution and is low in toxicity and residue and prone to degradation, and the dead rate of seedlings is 0 after the corn haploid is processed by a center dropping method with the special herbicide. The method is simple, convenient and efficient, and the doubling rate can reach 27.9%; and the doubling rate of the corn haploid processed by a bud immersion method with the special herbicide can reach 17.9%, and the dead rate of seedlings is obviously lower than that of the seedlings processed by colchicine. The method and the herbicide are suitable for large-scale operation, and can be applicable to large-scale breeding of the corn haploid.

Description

technical field [0001] The invention relates to a method for doubling corn haploid by using herbicide and special herbicide. Background technique [0002] In recent years, the large-scale application of maize haploid breeding technology based on induced lines has become one of the three core technologies of modern maize breeding comparable to molecular marker-assisted breeding technology and transgenic technology. Maize is the only crop that uses genetic methods to breed haploids on a large scale, but its natural and chemical doubling rates are still low, resulting in the loss of many haploid materials with excellent agronomic and yield traits due to failure to double, resulting in large Loss. [0003] At present, colchicine is mainly used for chemical doubling of corn haploids. This method has many disadvantages: it is easy to form chimeras during processing; it leads to abnormal growth, dead seedlings, and deformities of plants; the processing method is cumbersome and is ...

Claims

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Application Information

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IPC IPC(8): A01H3/04A01N57/06A01N37/18A01N33/18A01P13/00
Inventor 李建生惠国强赵贤容杜何为杨小红郑艳萍
Owner CHINA AGRI UNIV
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