71results about How to "Efficient method" patented technology

The invention provides a fucosyl transferase polypeptide, which can be applied to the synthesis of human milk oligosaccharides (HMOs), and belongs to the technical field of enzyme engineering. The fucosyltransferase polypeptide provided by the invention comprises polypeptides with at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99% and 100% same amino acid sequences as polypeptides shown in amino acid sequences such as SEQ ID NO: 1-25. According to the transferase polypeptide disclosed by the invention, the specificity of a polypeptide catalytic substrate is also improved while the ability of the polypeptide as shown in SEQ ID NO: 1 to catalyze and synthesize 2 '-FL is improved.

The invention relates to the technical field of heat exchangers, in particular to a plate heat exchanger cleaning system, a plate heat exchanger and a cleaning method. The fluid monitoring devices are installed at an inlet and an outlet of the plate heat exchanger respectively, connected with the control device and used for monitoring fluid data of the inlet and the outlet of the plate heat exchanger and transmitting the data to the control device. The high-pressure cleaning device is arranged between plate channels of the plate heat exchanger, is connected with the control device and is used for acquiring an instruction of the control device and cleaning the plate channels of the plate heat exchanger; the control device is used for receiving the fluid data of the fluid monitoring device and generating a cleaning instruction to the high-pressure cleaning device. Real-time monitoring and a response mechanism are combined, the fluid monitoring device, the high-pressure cleaning device and the control device are combined for control, the self-cleaning function of the plate heat exchanger is achieved, and the problems that an existing plate heat exchanger is difficult to clean and low in efficiency are solved.

The invention provides fluorescent conversion type red fluorescent powder for display as well as a preparation method and application thereof, and belongs to the technical field of light-emitting display materials. Metal Sr, metal Al and metal Eu are mixed and smelted according to the stoichiometric ratio and then ground to prepare alloy powder, lithium aluminum hydride powder and the alloy powder are ground, mixed, calcined and ground again, and the red fluorescent powder for laser display with high brightness under blue light excitation is prepared. According to the invention, an alloying method is combined with a nitriding method, and the molar ratio (greater than 70%) of Eu < 2 + > (radiative transition) in all Eu elements in the prepared fluorescent powder product is greatly increased, so that the prepared red fluorescent powder has high color purity, high luminous efficiency and thermal stability, and meanwhile, a sample has an ultrahigh saturation threshold value under the excitation of blue laser; the material can be applied to a fluorescence conversion type laser display technology, and has huge potential for laser display devices.

The invention relates to a series of GDP-fucose synthase polypeptides which can be applied to synthesis of GDP-fucose which is a precursor of human milk oligosaccharides (HMOs), and belongs to the technical field of enzyme engineering. In certain embodiments, the polypeptide capable of catalyzing the conversion of GDP-4-keto-6-deoxymannose into GDP-fucose comprises a polypeptide of which the amino acid sequence is at least 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99% and 100% identical to the amino acid sequence of SEQ ID NO: 1 and / or the amino acid sequence of the polypeptide M1-14. The invention provides a series of polypeptides for synthesizing GDP-fucose.

The invention provides a direct current charging pile charging method, system and device based on face recognition and a medium, and belongs to the technical field of charging piles. According to the scheme, the camera module is installed in the charging pile, the face image is collected in real time, and the main control module carries out face recognition through the YOLOv4 model. In the charging starting stage, the recognized face features are recorded, the charging pile is locked, face information is continuously detected during charging, and unlocking is conducted if the face information is matched with the face information. After charging is finished, charging is stopped, and unlocking is conducted. In addition, a charging abnormity detection function is introduced in the scheme, and charging abnormity is monitored in real time and processing measures are taken by collecting BMS and CCS data of the electric vehicle. Meanwhile, a face detection algorithm is optimized, an improved CSPDarkNet-53 is adopted as a backbone network, features are extracted through a CSPLayer2Conv convolution block, a PANet is replaced with an MFPN, and CCAM is introduced to enhance feature attention, so that the accuracy and robustness of the algorithm are improved, and the safety and convenience of the charging process are improved.

The invention relates to activity and fitness renewal. The present disclosure generally relates to navigating, viewing, and sharing campaign and fitness data, and interacting with fitness and / or campaign applications. In some examples, the scrolling activity data is based on content being displayed. In some examples, activity data for a friend may be viewed. In some examples, a notification and fitness data for a friend's completed fitness is received and displayed. In some examples, activity data received from friends is viewed and managed. In some examples, fitness data for multi-segment fitness is displayed in a three-dimensional stack on a map. In some examples, the fitness application operates in a restricted mode until a touch input having a characteristic intensity greater than a threshold intensity is received.

This invention discloses a method for preparing and applying a sodium borohydride-modified self-supporting anode for AEM water electrolyzers. Firstly, an amorphous ternary metal hydroxyl oxide precursor is grown in situ on a nickel substrate via room temperature liquid phase deposition. The process involves the presence of Co in solution. 2+ Fe 3+ Ni ions are introduced through slight corrosion of the nickel foam matrix. 2+ The ions, the three, and the OH- produced by hydrolysis ⁻ Co-deposition occurs under the influence of dissolved oxygen, leading to the self-assembly of amorphous nanosheet structures on the substrate surface. Subsequently, a mild chemical reduction treatment with sodium borohydride solution is used to obtain a structurally stable and high-performance self-supporting electrode. The method described in this embodiment is mild, simple, low-cost, and has good versatility.

This invention discloses a method for determining the content of Ophiopogonin C and Ophiopogonin D in Shengmai Yin soft capsules. The method involves simultaneous determination of the content of Ophiopogonin C and Ophiopogonin D in Shengmai Yin soft capsules using UPLC-MS / MS. An Agilent C18 column is used, with the mobile phase being a mixture of an aqueous solution containing 0.05% formic acid and 2 mmol / L ammonium acetate: acetonitrile-methanol (40:60, V / V) (30:70), at a flow rate of 0.3 mL / min. ESI-mode is used, with MRM monitoring of ion pairs. The test sample is prepared by reflux of 80% methanol for 15 min. This method is highly specific, sensitive, rapid, and accurate, effectively controlling the quality of Shengmai Yin soft capsules and filling the gap in the original standard for the quantitative determination of Ophiopogonin C and Ophiopogonin D.

The invention relates to a yeast gene modified cell containing GDP-fucose synthase polypeptide, which can be applied to synthesis of human milk oligosaccharide, and belongs to the technical field of gene engineering. According to the technical scheme, the yeast gene modified cell contains a heterologous nucleic acid sequence of a GDP-fucose synthase active polypeptide. The synthetase active polypeptide is obtained by modifying at least one site of the 20th site to the 29th site, the 40th site to the 53th site, the 75th site to the 81st site, the 95th site, the 148th site to the 158th site, the 193th site, the 215th site, the 219th site to the 21st site, the 227th site to the 232 site, the 239th site to the 241st site, the 259th site to the 264th site, the 301st site to the 302 site, the 306th site and the 310th site to the 316th site of an amino acid sequence shown in SEQ ID NO: 1. Compared with an original strain, the yeast gene modified cell has higher activity of synthesizing fucosyllactose, and a safe and feasible technical scheme is provided for industrial production of fucosyllactose.

The invention discloses a method for enhancing low-temperature biological denitrification of activated sludge, which comprises the following steps: operating a traditional nitrification and denitrification activated sludge reactor at 15 DEG C for 30 days, supplementing a matrix according to inlet water, then transferring to 4 DEG C to stress for 30 days, and supplementing any matrix at the stage, finally, the reactor is continuously operated according to a traditional nitrification and denitrification mode under the condition that the temperature is recovered to 10-15 DEG C, the nitrification effect of the reactor can be improved by 30.31%, the total nitrogen removal rate is improved by 28.57%, and an efficient and stable regulation and control method is provided for improving activated sludge biological nitrogen removal at low temperature.

This invention relates to the field of alloy purification technology, specifically to a method, system, and aluminum alloy welding material for preparation of self-purifying aluminum alloy welding material. The method for preparing the self-purifying aluminum alloy welding material includes the following steps: cold pressing a mixture of aluminum alloy powder and refining agent powder to obtain a cold-pressed billet; hot pressing the cold-pressed billet to obtain a hot-pressed aluminum ingot; removing impurities from the surface of the hot-pressed aluminum ingot, followed by preheating to obtain a preheated ingot; and extruding the preheated ingot into wire. This invention uses powder metallurgy to prepare aluminum alloy welding material with self-purifying function, extending the degassing method of aluminum alloy smelting to the aluminum alloy welding process. This relaxes the strict requirements on the hydrogen content of the aluminum alloy base material, achieving a weld with lower hydrogen content. The method is more efficient, more stable, and less expensive.

This invention belongs to the field of agricultural biotechnology, specifically relating to a plant constitutive promoter OsSULTR2;2pro and its applications. The nucleotide sequence of the promoter OsSULTR2;2pro is shown in SEQ ID NO.1. The promoter OsSULTR2;2pro of this invention is an endogenous constitutive promoter for rice, capable of driving efficient and stable expression of target genes in plant callus tissue, roots, stems, leaves during vegetative growth, as well as young panicles and seeds, and can replace existing non-plant-derived promoters. It has significant application value in the field of plant genetic engineering and can effectively reduce the potential safety risks of transgenic plants caused by the introduction of exogenous DNA.

The invention belongs to the field of organic synthesis, and discloses a method for realizing selective synthesis of cyano-substituted isoxazoline and 3H-pyrrole-3-ketone through temperature regulation, which comprises the following steps: by taking alkynyl ketoxime as a raw material, copper trifluoromethanesulfonate as a catalyst and cyanobenzo iodo-oxacyclopentanone (CBX reagent) as a cyanation reagent, reacting at the temperature of 20-50 DEG C under the condition that the temperature is 20-50 DEG C, thereby obtaining the cyano-substituted isoxazoline and 3H-pyrrole-3-ketone with the cyano-substituted isoxazoline and 3H-pyrrole-3-ketone. And carrying out tube sealing reaction in an inert gas atmosphere to obtain the cyano-substituted isoxazoline. When the temperature is increased to 100 DEG C to 120 DEG C, the reaction time is prolonged, and the cyano-substituted 3H-pyrrole-3-ketone is obtained. According to the invention, two different cyano-substituted nitrogen-containing heterocyclic compounds are selectively synthesized through temperature regulation and control and free radical cascade reaction of different paths. The reaction catalyst is less in dosage, the substrate is wide in application range, the operation is simple, and the method is efficient.

The application discloses a proteinase and coding gene and application for improving the resistance of potato to late blight, and belongs to the technical field of genetic engineering and plant disease-resistant breeding. The amino acid sequence of the proteinase StRD21 is shown as SEQ ID NO. 2, or a derivative sequence with the same disease-resistant function. The nucleotide sequence of the coding gene is shown as SEQ ID NO. 1. The application further provides a specific primer pair for cloning the gene, a potato recombinant overexpression vector containing the gene and a transgenic host cell. By introducing the StRD21 gene into potato and making the StRD21 gene overexpress, the resistance of potato to late blight can be significantly enhanced. The application provides an effective gene resource and a biotechnological means for cultivating a new variety of disease-resistant potato.

The invention discloses a circular hole detection and binocular matching method considering peripheral features. Comprising the following steps: constructing a three-dimensional part model according to structure parameters of a to-be-detected part, and constructing a data set through a rendering method by utilizing the three-dimensional part model; constructing a circular hole detection and binocular matching model, and training by using the data set to obtain a trained circular hole detection and binocular matching model; the circular hole detection and binocular matching model comprises a feature extraction backbone network, a feature encoder, two parallel feature decoders and a feature segmentation module, and the two feature decoders are respectively a detection decoder and a matching decoder; and acquiring an actual binocular image of a to-be-detected part, and inputting the actual binocular image into the trained round hole detection and binocular matching model to obtain a round hole detection result. According to the method, the position relation between the circular holes and peripheral features such as the environment can be effectively utilized, and the accuracy and robustness of binocular matching and segmentation in a complex scene are improved.

This invention provides a road network-level pavement technical condition detection path planning method, comprising: determining the current mileage marker coordinates corresponding to the road network to be detected based on the number of detection vehicles; determining all detection road segments corresponding to the current mileage marker coordinates based on the current mileage marker coordinates; determining recommended detection road segments from all detection road segments; traversing all remaining mileage marker coordinates, and determining the planned path corresponding to the road network to be detected based on the recommended detection road segments. The road network-level pavement technical condition detection path planning method provided by this invention divides the road network to be detected into several detection road segments with mileage marker coordinates as nodes, and uses these detection road segments as basic traversal units to traverse all detection road segments, ultimately determining the final planned path. This method avoids the drawback of detection vehicles traversing numerous backtracks, reducing detection time and labor costs; furthermore, this method is more scientific and efficient.

The invention discloses an electroencephalogram emotion recognition method and device based on comparative learning and a graph neural network. The method comprises the steps that firstly, data preprocessing is conducted on electroencephalogram signals, baseline signals are removed, and time sequence segmentation and baseline effect removal are conducted; then constructing a node feature extraction module, and extracting time and space features by using time convolution and depth separable convolution; next, sampling and aggregating neighbor node features on the complete undirected graph by using a GraphSAGE algorithm, and generating graph-level features through a full connection layer; then, constructing a comparative learning module, generating a triple sample pair according to the graph-level features, carrying out triple comparative learning, and selecting a semi-hard negative sample for training; and finally, constructing a classifier through a full connection layer and a softmax function to perform emotion classification. According to the method, the time and space features and the graph structure information of the electroencephalogram signals are effectively extracted and utilized through the method combining comparative learning and the graph neural network, and the accuracy of emotion recognition is improved.

This disclosure relates to user interfaces for audio media controls. A method includes displaying a first media information affordance representing a first media item in response to detecting an indication that a physical proximity between an electronic device and an external device satisfies a proximity condition. The electronic device receives a first input representing a selection of the first media information affordance. In response to receiving the first input: in accordance with a determination that the first input is of a first type of input, initiating a process to play back the first media item; and in accordance with a determination that the first input is of a second type of input that is different from the first type of input, displaying a second media information affordance representing the first media item.

The invention discloses a method for synthesizing functional polyether in a multi-proton system, which comprises the following steps: mixing an epoxy monomer, an amino alcohol / acid compound, organic alkali and alkyl boron for polymerization reaction to obtain carbamate / amide / oligopeptide functional polyether. According to the invention, the hydroxyl is preferentially activated in a multi-proton system by utilizing an acidic reversal phenomenon of an NH type proton group and the hydroxyl in a double-component organic catalytic system, so that a polymerization reaction is only carried out at a hydroxyl / carboxyl terminal; and finally, carbamate / amide / oligopeptide functional polyether with controllable molecular weight and a clear end group structure can be synthesized in a site selective manner through a one-step reaction, and the method is simple, convenient, efficient, controllable and suitable for large-scale industrial application.

This invention discloses an immune cell with enhanced anti-tumor effect, its preparation method, and its application. Experiments using this invention show that... SERTAD1 CD8 gene overexpression + T cells can enhance their function in anti-tumor immunotherapy. SERTAD1 Gene overexpression can downregulate CD8 + The expression of immune checkpoint molecules on T cells upregulates the expression of mRNAs of tumor-killing cytokines. In the MC38-OVA subcutaneous tumor model, Naïve CD8 cells were extracted from OT-I mice. + T cells were infected with a virus to overexpress SERTAD1, followed by adoptive transfer to mice. The results showed that SERTAD1 overexpression enhanced CD8 expression. + T cells kill MC38-OVA cells, inhibit the growth of MC38-OVA tumor cells, and overexpress SERTAD1 CD8. + The tumor volume and weight in the T-cell adoptive group were significantly smaller than those in the control group.

The invention relates to a method and interface for home media control. The present disclosure relates generally to interfaces and techniques for media playback on one or more devices. According to some embodiments, an electronic device includes a display, one or more processors, and a memory. The electronic device receives a user input and, in response to receiving the user input, displays a multi-device interface on the display, the multi-device interface comprising: one or more indicators associated with a plurality of available playback devices and media playback conditions of the plurality of available playback devices, the plurality of available playback devices are connected to the device and are available to initiate playback of media from the device.

The invention relates to a method and interface for home media control. The present disclosure relates generally to interfaces and techniques for media playback on one or more devices. According to some embodiments, an electronic device includes a display, one or more processors, and a memory. The electronic device receives a user input and, in response to receiving the user input, displays a multi-device interface on the display, the multi-device interface comprising: one or more indicators associated with a plurality of available playback devices and media playback conditions of the plurality of available playback devices, the plurality of available playback devices are connected to the device and are available to initiate playback of media from the device.

This invention discloses a rapid test card for ABO, RhD blood typing and sickle cell anemia, comprising a card holder, a card cover, an ABD test strip, and an SCD test strip. The ABD and SCD test strips are installed in the card holder, and the card cover is affixed to the card holder. This invention can simultaneously detect ABO, RhD blood typing, and SCD. By improving sample processing, test point layout, and test strip design, it overcomes the shortcomings of existing technologies, significantly improving the accuracy, simplicity, and efficiency of the test, making it particularly suitable for point-of-care testing.

The invention discloses a method for improving seed germination under a salt stress condition and application thereof, and belongs to the technical field of crop production. According to the method provided by the invention, the germination ability of the seeds under the salt stress condition can be remarkably improved, the germination rate, germination potential and germination index of the watermelon seeds and the muskmelon seeds under the salt stress condition simulated by 250mM NaCl can be remarkably improved by soaking the seeds with 10 mu M methyl caffeate, and the germination of the watermelon seeds and the muskmelon seeds under the normal condition is not influenced; seedlings generated by the seeds treated by the method provided by the invention grow robustly, and compared with seedlings grown under normal conditions, obvious abnormity is not observed, so that the method provided by the invention is safe and has no toxic effect on plant growth; the provided method is simple, effective and low in cost, and can be used for improving the seed germination capacity under the salt stress condition so as to improve the utilization capacity of the saline-alkali soil and improve the crop yield and quality contribution of the saline-alkali soil.

The invention relates to the technical field of nylon, and in particular to a core-shell structure modified nylon plastic and a preparation method thereof. The core-shell structure modified nylon plastic comprises the following raw materials in parts by weight: 100-150 parts of nylon, 20-40 parts of silicon copolymer polycarbonate, 5-9 parts of dimethyl silicone oil, 10-15 parts of a core-shell structure modifier, 0.5-1 parts of a vulcanizer and 0.5-1.5 parts of a dispersant; wherein the core-shell structure modifier is a core-shell structure with olefinic silica microspheres as cores and single-end hydrogen-containing silicone oil as shells, and the olefinic silica microspheres are obtained by grafting vinyl tris(β-methoxyethoxy) silane onto silica microspheres. The core-shell structure modified nylon plastic of the invention can be used for manufacturing high-performance engineering plastics, such as automotive parts, electronic devices and the like, and has broad market application prospects.

The application discloses a star map blind restoration method when star points are in elliptic arc trajectories under compound motion with three-axis angular velocities all being non-zero, which comprises the following steps: S1, elliptic fitting is performed on the elliptic arc trajectories of the star points; S2, elliptic arc trajectory and circular arc trajectory transformation based on projection transformation is performed; S3, circular arc trajectory and straight line segment trajectory transformation based on polar coordinate transformation is performed; S4, straight line segment trajectory restoration based on motion blurred image blind restoration is performed; S5, coordinate inverse transformation and projection inverse transformation are performed to obtain the final restoration result of the star map with the original trajectory being the elliptic arc trajectory. The application realizes the star map blind restoration of the elliptic arc trajectory by transforming the elliptic arc trajectory into the circular arc trajectory through the projection transformation, transforming the circular arc trajectory into the straight line segment trajectory through the polar coordinate transformation, and then realizing the star map restoration of the elliptic arc trajectory through the straight line segment restoration and the coordinate inverse transformation.

The invention relates to a method and interface for home media control. The present disclosure relates generally to interfaces and techniques for media playback on one or more devices. According to some embodiments, an electronic device includes a display, one or more processors, and a memory. The electronic device receives a user input and, in response to receiving the user input, displays a multi-device interface on the display, the multi-device interface comprising: one or more indicators associated with a plurality of available playback devices and media playback conditions of the plurality of available playback devices, the plurality of available playback devices are connected to the device and are available to initiate playback of media from the device.

The invention belongs to the field of semiconductors, and particularly relates to a method for reducing the K value of a low-K silicon oxide material. The method comprises the following steps: (1) transferring a wafer deposited with a low-K silicon oxide material to a reaction cavity; (2) preheating the wafer on which the low-K silicon oxide material is deposited; (3) igniting the remote plasma device by using argon; and (4) gradually reducing the flow of the argon and gradually increasing the flow of the hydrogen until the flow of the argon is zero, so that the low-K silicon oxide material is continuously treated by the hydrogen. According to the method, the configuration of carbon in the low-K silicon oxide material is changed through the remote hydrogen plasma, so that the K value of the low-K silicon oxide material is reduced. The remote hydrogen plasma technology is a brand new method for reducing the K value of the low-K silicon oxide material, the method is simple, direct and efficient and does not need chemical additives, new pores cannot be introduced into the low-K silicon oxide material in the process, and the method provides a new solution for solving the problem of the low-K silicon oxide material.

The present disclosure generally relates to managing health data of a patient. In some embodiments, the disclosed technology includes displaying a graphical representation of data, the graphical representation of data including a first plot corresponding to a first data set and a second plot corresponding to a second data set. An input directed to the first plot is detected, and in response, a plurality of user interface objects is displayed, the plurality of user interface objects including a first user interface object or a second user interface object. The first user interface object is associated with the first plot and based on a first variable, the first variable being selected based on a location of the input, and the second user interface object is associated with the second plot and based on a second variable, the second variable being selected based on the location of the first input.

The invention relates to a shaft-bearing bush part friction wear performance rapid test method, which utilizes a shaft-bearing bush friction wear testing machine to carry out experiments, adopts a finished product bearing bush in practical application, and uses a straight shaft to simulate a crankshaft journal, and the material and the surface treatment process of the straight shaft are consistent with those of the crankshaft journal. The test is carried out under the conditions of low speed (1-500rpm) and heavy load (5-120MPa), so that the shaft-bearing bush can relatively slide in a boundary lubrication or mixed lubrication state, rapid and stable wear is realized, and the method can rapidly evaluate the friction wear performance of the shaft-bearing bush at the part test level. The device has high flexibility, and shafts and bearing bushes of different materials, different sizes and different surface treatment processes can be replaced according to different requirements. The test result can be quickly obtained, the stability and repeatability are good, and a reliable method is provided for testing the friction and wear performance of the shaft-bearing bush. The application of the method can enhance the performance analysis and optimization of the shaft-bearing bush system.