571results about How to "Quick sorting" patented technology

The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. In certain embodiments the methods make use of extension probes containing an abasic residue or a damaged base and employ agents appropriate to cleave linkages between a nucleoside and an abasic residue and / or agents appropriate to remove a damaged base from a nucleic acid. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to beads, which are immobilized in or on a semi-solid support. The invention further provides sets of labeled extension probes containing phosphorothiolate linkages or trigger residues that are suitable for use in the method. In addition, the invention includes performing multiple sequencing reactions on a single template by removing initializing oligonucleotides and extended strands and performing subsequent reactions using different initializing oligonucleotides. The invention further provides efficient methods for preparing templates, particularly for performing sequencing multiple different templates in parallel. The invention also provides methods for performing ligation and cleavage. The invention also provides new libraries of nucleic acid fragments containing paired tags, and methods of preparing microparticles having multiple different templates (e.g., containing paired tags) attached thereto and of sequencing the templates individually. The invention also provides automated sequencing systems, flow cells, image processing methods, and computer-readable media that store computer-executable instructions (e.g., to perform the image-processing methods) and / or sequence information. In certain embodiments the sequence information is stored in a database.

The present invention provides assay systems and related methods for determining genetic abnormalities in mixed samples comprising cell free DNA from both normal and putative genetically atypical cells. Exemplary mixed samples for analysis using the assay systems of the invention include samples comprising both maternal and fetal cell free DNA and samples that contain DNA from normal cells and circulating cancerous cells.

The present disclosure provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles typically comprise steps of extension, ligation, and cleavage. In certain embodiments, the methods make use of extension probes containing phosphorothiolate linkages and agents capable of cleaving such linkages. Methods of determining information about a sequence using at least two distinguishably labeled probe families are provided, as are methods of performing multiple sequencing reactions on a single template. Automated sequencing systems, flow cells, image processing methods, and computer-readable media that store computer-executable instructions and / or sequence information that can be used in accordance with such methods are also provided. In certain embodiments, blocking oligonucleotides are provided to facilitate sequencing using disclosed methods.

The present invention provides assay systems and related methods for determining genetic abnormalities in mixed samples comprising cell free DNA from both normal and putative genetically atypical cells. Exemplary mixed samples for analysis using the assay systems of the invention include samples comprising both maternal and fetal cell free DNA and samples that contain DNA from normal cells and circulating cancerous cells.

A method for retrofitting DNA in a single-copy or high-copy vector, such as a fosmid or BAC, whereby an artificial transposon is used to introduce a conditional multi-copy origin of replication (“ori”) into the DNA in said vector. Following random in vitro or in vivo transposition of the ori-containing transposon into DNA in the single-copy or low-copy vector, the resulting insertion clones are introduced into a special host strain that contains a gene which encodes a polypeptide required for replication from the multi-copy ori. However, since the gene for this polypeptide is expressed from a tightly-regulated inducible promoter, the polypeptide is not expressed in the absence of inducer. On addition of inducer to the culture medium, the host cell synthesizes the polypeptide, which in turn activates replication from the multi-copy ori, thereby increasing the amount of clone DNA synthesized by the cell.

The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to immobilized beads. The invention further provides sets of labeled probes containing phosphorothiolate linkages. In addition, the invention includes performing multiple sequencing reactions on a single template by removing initializing oligonucleotides and extended strands and performing subsequent reactions using different initializing oligonucleotides.

A system and method for online, essentially instantaneous analyses of ore compositions on a moving belt use laser-induced break-down spectroscopy (LIBS), wherein intensity ratios of emission lines characteristic of specific minerals or ions are calculated on areas of the moving belt, with elevated contents of identifying ions or species being detectable. The ratios are derived from ions present in the sample that differentiate a first substance from a second substance, thus allowing sorting of the samples. The substance may include an ion, an element, or a compound.

The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to immobilized beads. The invention further provides sets of labeled probes containing phosphorothiolate linkages. In addition, the invention includes performing multiple sequencing reactions on a single template by removing initializing oligonucleotides and extended strands and performing subsequent reactions using different initializing oligonucleotides.

The present invention relates to the field of property or real estate. In one form, the invention relates to the rating of property. In another form, the present invention relates to the ranking of property based on various inputs into the system and method of the present invention. The rating and / or ranking is determined in accordance with a numerical value.

The invention discloses a query method, a query system and a query device based on vertical search. The method comprises the following steps of: acquiring the query information of a user; retrieving a category model which is matched with the query information in a category model library according to the query information, and generating a first query result according to the retrieved category model, wherein the category model comprises goods categories corresponding to keywords which are input by the user; searching the goods category which is matched with the query information in a goods library according to the query information to generate a second query result; and combining the first query result and the second query result to generate a final query result. By the invention, relevancy between the query results of the user and the query intention of the user is improved, and user experience is improved.

The invention is directed to methods for determining clonotypes and clonotype profiles in assays for analyzing immune repertoires by high throughput nucleic acid sequencing of somatically recombined immune molecules. In one aspect, the invention comprises generating a clonotype profile from an individual by generating sequence reads from a sample of recombined immune molecules; forming from the sequence reads a sequence tree representing candidate clonotypes each having a frequency; coalescing with a highest frequency candidate clonotype any lesser frequency candidate clonotypes whenever such lesser frequency is below a predetermined value and whenever a sequence difference therebetween is below a predetermined value to form a clonotype. After such coalescence, the candidate clonotypes is removed from the sequence tree and the process is repeated. This approach permits rapid and efficient differentiation of candidate clonotypes with genuine sequence differences from those with experimental or measurement errors, such as sequencing errors.

The invention discloses a method and device for determining pushed information. The pushed information is data information for describing a pushed object, the method comprises the steps of determining a reference pushed object according to user information and / or website content information when detecting that a user browses website contents; determining at least one object cluster corresponding to the reference pushed object, wherein the object cluster is a set of a plurality of pushed objects obtained through the clustering algorithm of a text correlation value and a behavior correlation value of the pushed objects; obtaining a target key word corresponding to at least one object cluster, and obtaining the pushed information from a information pushing system according to the target key word. The method and device for determining the pushed information can improve the consistency of the pushed information and the potential requirement of a user and improve precision of information pushing.

Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.

The invention discloses an on-line rapid non-destructive detection and sorting device for slightly damaged fresh dates. The device comprises a mechanical conveying device, a rotation device, a visible near-infrared spectrum acquisition device and a sorting device, wherein the mechanical conveying device is used for conveying fresh dates to a transmission roller at the station of the visible near-infrared spectrum acquisition device; the rotation device is used for enabling the roller to drive the fresh dates to rotate for 360 degrees; the position information of the fresh dates is judged through a sensor; the position information is transmitted to a micro optical fiber spectrometer through an optical fiber; the acquired spectrum information is transmitted to a model for judging whether the fresh dates are slightly damaged or not in computer system software; a signal that whether the fresh dates are slightly damaged is transmitted to a PLC (programmable logic controller) through the software in a computer; an electromagnetic control valve and a whole sorting execution mechanism which is connected with an air compressor are controlled by the PLC, and thus automatic sorting on slightly damaged fresh dates and complete fresh dates is achieved. By adopting the device, rapid, non-destructive, dynamic and real-time detection and sorting on slightly damaged fresh dates can be achieved.

A method for determining a genome sequence comprising the steps of digesting the genome with at least one first restriction endonuclease, ligating at least one adaptor to the restriction fragments of the first subset, selectively amplifying the first set of adaptor-ligated restriction fragments using a first primer combination wherein at least a first primer contains a first selected sequence at the 3′ end of the primer sequence, comprising 1-10 selective nucleotides, repeating these steps with at least a second primer combinations wherein the primer contains a different second selected sequence, fragmenting each of the subsets of amplified adaptor-ligated restriction fragments to generate sequencing libraries, determine the nucleotide sequence of the fragments, aligning the sequence of the fragments in each of the libraries to generate contigs, repeating these steps for one second and / or further restriction endonucleases, aligning the contigs obtained for each of the second and / or further restriction endonucleases to provide for a sequence of the genome.

The invention discloses a method for arranging steering buses in sequence in a bus stop at the inner side of a road. The method comprises the steps that according to collected bus real-time information, time when one bus reaches a downstream intersection is predicted, passenger delay caused by steering and a signal phase sequence of the bus is analyzed, so that an optimal scheme of the bus sequence is generated, a driving waiting zone and a signal control light are arranged at the bus stop at the inner side of the road, and therefore the bus operation sequence is effectively adjusted. According to the method for arranging the steering buses in sequence in the bus stop at the inner side of the road, from the perspective of bus operation time and with the combination of bus operation experience data and real-time collected data, the time when one bus reaches the downstream intersection is accurately predicted, passenger delay caused by the driving sequence of the steering buses is accurately judged according to an original green light interval time table, and the reliable steering bus sequence is formed; the driving waiting zone is arranged at the bus stop, so that under the condition of linkage control of an outlet portion and the signal light of the driving waiting zone, the buses are rapidly and effectively lined, total delay of the steering buses in the intersection is reduced, prior passage of the buses at the signal intersection of the downstream is guaranteed, and the operation efficiency of the buses is effectively improved.

The invention discloses a logistic commodity sorting device and system. The logistic commodity sorting device comprises an upper computer processing center, a conveying belt device, an automatic scanner device, a signal detection device, a sorting power component device and separating channels. When logistic commodities are placed on the conveying belt device, the automatic scanner device reads information of the logistic commodities and transmits the information to the upper computer processing center, the signal detection device transmits the information to the upper computer processing center when detecting the information of the logistic commodities, the upper computer processing center sends action commands according to the obtained information, the sorting power component device executes the action commands and pushes the corresponding commodities into the corresponding separating channels, and therefore the logistic sorting process can be realized. According to the technical scheme, the logistic commodities can be effectively, quickly and accurately sorted. The logistic commodity sorting device and system are suitable for the sorting work of medium-small logistic sorting stations and can improve the logistic sorting efficiency, lower the labor intensity and lower the labor cost.

The invention relates to an evaluation method of the power supply reliability of a distribution network based on demand response. The method includes steps: 1) building a demand response model based on time-of-use power price; 2) building an improved load optimization model based on demand response; 3) performing optimization and classification of an optimized load curve by employing a bisection method, and solving the optimal classification number; and 4) establishing an improved cluster load analysis reliability evaluation algorithm based on the bisection method; and changing a cluster load interval into a monotonic increasing trend for convenient calculation. According to the method, the problem of the evaluation of the power supply reliability of the distribution network based on demand response is effectively solved.

The invention discloses a micro luminous display device and a manufacturing method thereof. The manufacturing method comprises the following steps: providing a tank containing a liquid; disposing at least a carrying plate with several recessed regions in the tank, and the carrying plate being immersed in the liquid; dropping several lighting devices into the liquid, wherein each of the lighting devices includes at least two conductive pads, and one of the two conductive pads includes a magnetic material; applying a magnetic field for the lighting devices and the lighting devices will dispose within the recessed regions of the carrying plate; removing the carrying plate with the lighting devices out of the tank, and electrically assembling the lighting devices to an array substrate.

The invention discloses a hair ring split charging machine. At present, ring packaging is manually operated, and the packaging efficiency is low. The hair ring split charging machine comprises a feeding hopper, a rotation mechanism, a transverse moving screening mechanism, a material dividing device, counters and a discharging device; the rotation mechanism is arranged at the bottom of a discharging hopper, the transverse moving screening mechanism is arranged below the rotation mechanism, and raw materials are evenly discharged; three split strips are arranged between a conveying belt mechanism of the material dividing device and a screen of the transverse moving screening mechanism, the position, between every two adjacent split strips, of the output end of the conveying belt mechanism is provided with one counter, and the discharging device is arranged below the counters and comprises a discharging barrel, first rotating shafts, second rotating shafts and a flow combining part; and the discharging barrel is divided into two discharging chambers, first discharging clamping rings are fixed to the first rotating shafts in the discharging chambers, clamping grooves in the tops of the first discharging clamping rings are fixed to first baffles, second discharging clamping rings are fixed to the second rotating shafts, and two second baffles are fixed in two clamping grooves in the bottoms of the second discharging clamping rings. A gap of flexible material split charging in industrial production is filled up by means of the hair ring split charging machinej.

The invention relates to the bearing trench automatic selecting system. It aims to the big scatter of inside and outside liners of the bearing in machining, randomly selecting with low matching rate issue, starting with categorization of dimensions of the trenches, selecting rolling ball and the inner liner based on the dimension of the trench size. Currently, manual selection is used with big precision and efficiency change rate. It comprises the selection glide path on the fixed frame, drive mechanism fixed on the frame, support and drive mechanism fixed on the said frame, storage cabin installed inside the frame. It is selected based on the automatic measurement of the inside and outside trench dimensions.

The invention discloses a rib marking method, device and equipment and a training method of an image segmentation model, and the method comprises the steps: obtaining a to-be-identified image; Whereinthe image to be identified comprises a CT image; Inputting the to-be-identified image into an image segmentation model to obtain a segmentation result; Wherein the segmentation result comprises a ribsegmentation result and a rib center line segmentation result; And performing rib sorting and marking based on the segmentation result. According to the invention, rib adhesion and fracture conditions can be well handled, and each rib can be quickly sorted and marked.

The invention discloses a sorting robot of a laser cutting machine and a part sorting method of the sorting robot and belongs to the field of machine tools. A Y-axis support seat comprise a Y-axis longitudinal support seat and a Y-axis transverse support seat; the Y-axis longitudinal support seat is matched with a rail on an X-axis support seat through a sliding mechanism; a Z-axis support seat and the Y-axis transverse support seat are connected through a connecting piece; a C-axis support seat is matched with the Z-axis support seat; the C-axis support seat is driven by a driving device and is connected with a sorting tool; the sorting tool comprises a master panel; a plurality of air cylinders are arranged on the master panel in an array; and each air cylinder is separately connected with a sucking disc. The sorting robot of the laser cutting machine can replace the laser cutting machine for manual sorting, can identify a finished product cut by laser, and rapidly sort and stack the cut finished product.

The present invention discloses a flash conversion layer control method based on request classification. The method comprises the following steps: Step S1: setting a plurality of address mapping cachetables in a memory according to an operation request of the file system and an address request frequency; Step S2: obtaining the operation request of the file system in the flash conversion layer andparsing the operation request to determine the operation request type; Step S3: searching the logical page address of the operation request in multiple address mapping cache tables in different priority orders according to the operation request type until hitting the corresponding address mapping entry; and Step S4: updating the memory address mapping cache tables according to the operation request result. Compared with the prior art, according to the method disclosed by the present invention, the request is divided in a more fine-grained manner, quickly hitting the mapping entry is facilitated, and when removing the mapping entry, the classified removal can be carried out to accelerate the removal speed, the mapping entries that must be updated can be quickly sorted, and the update of the mapping entries that does not require to be updated is avoided.

A mail processing system including a franking machine for franking mail items, which franking machine is connected to a first server that is itself connected to a second server in communication with a sorting machine, the franking machine including scanner means for scanning the destination addresses borne on the mail items as said mail items are inserted, storage means for storing the digital images of the addresses scanned in this way, in association with respective unique identification numbers, and communications means for periodically transferring the digital images as indexed in this way to the first server, the first or second server further including means for extracting determined data from the digital images, which data, associated with said unique identification numbers, is suitable for enabling said sorting machine to be controlled automatically on receiving said franked mail items.