15853 results about "Agricultural science" patented technology

The present invention provides cotton plants, cotton tissues, and cotton seeds that include the MON15985 event, which confers resistance to Lepidopteran insect damage. Also provided are assays for detecting the presence of the MON15985 event based on the DNA sequence of the recombinant construct inserted into the cotton genome that resulted in the MON15985 event and/or the genomic sequences flanking the insertion site.

A multi-zone landscape irrigation and lighting system includes a personal computer equipped with a detachable wireless remote, and a multi-station controller operating a plurality of irrigation valves and lighting circuits in accordance with schedules wirelessly transmitted to it by the computer through the remote. When detached from the computer, the remote can wirelessly operate selected irrigation or lighting zones manually. The computer has an intuitive graphic user interface capable of using actual zone photographs to identify individual zones in a strip of zone icons. The icon strip is movable past a lens-like window, the zone icon under the lens at a given time determining the zone currently programmable. A residential system can be operated by a contractor from the street, and the system can be programmed by e-mail from the contractor's shop. Fully automatic operation may be derived from data obtained over the Internet.

A method, and systems implementing such method, for reducing methane emissions by ruminants. The method includes providing a feed dispenser for feeding ruminants one or more nutrient supplements, and the feed dispenser includes a gas analyzer proximate to where the ruminant places its head. The method includes determining a particular ruminant has accessed the feed dispenser such as by reading an identifier from an RFID ear tag and operating the feed dispenser to provide a ration of methane-controlling nutrient supplement. The method includes using the gas analyzer to determine levels of carbon dioxide and methane and operating a data analyzing station to determine a ratio of methane to carbon dioxide and modify the type or amount of nutrient supplement for the ruminant for a next feeding to control methane production or achieve an animal production goal, such as by selectively operating a hopper with two or more supplements compartments.

The present invention relates to systems and methods for monitoring agricultural products. In particular, the present invention relates to monitoring fruit production, plant growth, and plant vitality.

The invention discloses a CAR-T transgene vector based on replication defective recombinant lentivirus. The CAR-T transgene vector comprises an original nuclear replicon pUCOri sequence, a resistance gene AmpR sequence containing ampicillin, a virus replicon SV40 Ori sequence, a lentivirus packaging cis element, ZsGreen1 green fluorescent protein, an IRES ribosome binding sequence, a human EF1 alpha promoter , a chimeric antigen receptor of second-generation CAR or third-generation CAR and a regulating element, wherein the original nuclear replicon pUCOri sequence is used for plasmid replication; the resistance gene AmpR sequence is used for massively proliferating target strains; the virus replicon SV40 Ori sequence is used for enhancing replication in eukaryocyte; the lentivirus packaging cis element is used for lentivirus packaging; the ZsGreen1 green fluorescent protein is used for expressing green fluorescent for eukaryocyte; the IRES ribosome binding sequence is used for jointly transcribing and expressing protein; the human EF1 alpha promoter is used for conducting eukaryotic transcription on antigen receptor genes; the chimeric antigen receptor is used for forming the second-generation CAR or the third-generation CAR integrating recognition, transfer and start; the regulating element is used for enhancing expression efficiency of transgenes and used after eWPRE-enhanced type woodchuck hepatitis b virus is transcribed. Besides, the invention further discloses a construction method and application of the vector. By means of the CAR-T transgene vector and the construction method and application of the vector, secretion of cell factors and an in vitro killing effect of CAR-T cells can be remarkably improved, and the clinical treatment effect is remarkable.

The invention discloses a processing method of nutritional composite rice. The processing method comprises the steps of: firstly, crushing raw materials, such as paddy rice and coarse cereals, in a formula, then adding water according to proportion for mixation, feeding the well mixed mixture into a rice making machine to prepare rice grain shaped composite rice, cooling, sterilizing by microwaves, cooling again, finally feeding into a finished product warehouse, and packaging according to requirements. The nutritional composite rice disclosed by the invention not only preserves the nutritionof rice and wheat, but also contains the nutrition of various coarse cereals, and simultaneously, is good in mouth feel, easy to steam and cook, simple in process and easy to operate.

A system for controlling crop transfer from a self-propelled agricultural harvesting machine into the loading container of a hauling vehicle includes a transfer device assigned to the harvesting machine from which the crop emerges in the form of a crop discharge flow and a detection device which detects the position of the loading container relative to the harvesting machine and transmits it to a control unit. The control unit actuates at least one actuator assigned to the transfer device in such a way that the crop discharge flow lands within the loading container in order to fill it with crop. The control unit generates a control signal (S) for the hauling vehicle that specifies a position of the hauling vehicle relative to the harvesting machine that is suitable for transfer into the loading container.

The invention provides a distributed-network automatic irrigation control system and an irrigation control method thereof, wherein the system carries out automatic irrigation control on agricultural, forest, commercial, industrial or urban green areas by using the combined technical means such as intensively setting and monitoring a distributed irrigation process by a central control computer, controlling the distributed irrigation process by a wireless terminal controller, a wireless network communication technique and the like; and in addition, the system also enables the data processing pressure of the central control computer to be decomposed by using a distributed irrigation control method which is implemented through the steps of intensively setting the irrigation process by the central control computer, and independently controlling each unit irrigation process by the wireless terminal controllers according to the setting, thereby avoiding a mass of real-time communication data transmission, ensuring that the system can smoothly carry out data processing and communication, improving the execution efficiency and management efficiency of the system, decreasing the construction requirements of system hardware and wireless communication networks, and enhancing the economical efficiency and feasibility of the system applied in large-scale irrigations.

The invention provides a NP chip used for identifying rice variety. The chip comprises 3072 molecular markers, and the 3072 molecular markers are shown in SEQ ID NO. 0001- SEQ ID NO. 3072. The invention also provides a preparation method for the above SNP chip, and the preparation method comprises the steps: acquiring the SNP molecular markers from rice cultivar resequencing data, screening the SNP molecular markers and employing the screened molecular markers to design a probe and prepare the SNP chip. The invention also provides application of the above SNP chip to rice variety authenticity and identity identification. The provided application of the combination of the 3072 SNP molecular markers helps to greatly improve the detection accuracy, shorten the detection time and substantially improve our country identification technology level on rice variety.

The invention discloses a standardized cultivation method of super double cropping rice. Currently, the super double cropping rice is cultivated still according to a traditional method for cultivating ordinary rice, yield is not stable, the quality is not high, and grain safety and income increasing of farmers are seriously affected. The standardized cultivation method includes the technical measures of selection of varieties, selection of environment of producing area, robust seedlings cultivation, field cultivation and management, integrated control over plant diseases and insect pests and the like, and systematized standardized cultivation technology integration research of the super double cropping rice is carried out. According to a test result after 56 thousand of hm2 is popularized and applied, the yield of 667 m<2> reaches 456.67 kg/ 667 m<2> averagely, the yield increase range reaches 42.57 kg/ 667m<2> averagely, the rate of growth reaches 10.27% averagely, the increased income of every 667 m<2> reaches 100.58 RMB/ 667 m<2> averagely, the cost for using chemical pesticide to control diseases, pests and the like is lowered, and the cost is saved by 6.5-7.2 RMB/ 667 m<2>. According to the technical standard, the production of the super rice is indexed and scientized, the technical operability is high, the application effect is good, the standardized cultivation method is deeply popular by mass farmers, and the gained economic benefit, social benefits and ecological benefits are remarkable.

The present invention discloses a new-type snuff and its preparation method. It is made up by using dried tobacco leaf, dried natural vanilla and functional auxiliary material according to the mixing ratio of 1:0.8:0.2 through a certain preparation process. It can greatly reduce nicotine, tar and other harmful matter contents.

The invention discloses a cow fine breeding method based on the RFID technology. The method includes the following steps that 1, the milk production of cows is recorded through an automatic milk production recording subsystem where the RFID technology and milking are combined; 2, automatic clustering management is performed on the cows through an automatic cow isolation door based on the RFID technology; 3, appropriate nutritious daily ration is prepared for cow groups through a TMR monitoring subsystem based on the RFID technology; 4, cow movement and oestrus sensors are installed on cow neckbands so as to monitor movement conditions of the cows in real time. In the cow fine breeding method, the cow breeding management business relates to various fields of milking management and fodder management, and the cow fine breeding method is applied and deployed from the aspects of automatic milk production counting, clustering management, fodder management and oestrus hybridization management according to different business functions of cow farms.

The invention discloses a pleurotus geesteranus medium and a preparation method thereof. The recipe of the medium comprises (by weight) 60-75% of cotton seed hull or wheat straw or rice straw or corn cob, 7-15% of weed tree sawdust, 8-15% of wheat bran or rice bran, 3-8% of corn flour, 0.5-1.5% of sucrose, 1-1.5% of dried gypsum, 2-2.5% of quicklime, 0.1-0.3% of potassium dihydrogen phosphate and 0.1-0.2% of magnesium sulfate. The preparation method of the medium comprises the following steps: dissolving the quicklime, the dried gypsum, the potassium dihydrogen phosphate and the magnesium sulfate into water; adding the cotton seed hull or the wheat straw or the rice straw or the corn cob and the weed tree sawdust for prewetting; then adding the rice bran, the corn flour, the wheat bran and the sucrose and mixing evenly; and returning the medium having been used for planting the pleurotus geesteranus to field and soil in situ. The technical scheme can cause the pleurotus geesteranus to grow better and avoids the defect of no special medium for the growth of the pleurotus geesteranus in the prior art.

A method for breeding beef cattle and mutton sheep standardly on a large scale through photovoltaic facilities includes the four parts that first, a photovoltaic facility greenhouse is built, maize seedlings or high-quality green forage grass Zea mexicana is produced throughout the year to be used as green fodder of beef cattle and mutton sheep; second, the beef cattle and the mutton sheep are bred and bio-organic fertilizer is produced standardly on a large scale through the photovoltaic facility greenhouse; third, photovoltaic power generation is performed for self use, and excessive electricity is connected into the state grid; fourth, a beef cattle and mutton sheep traceable system is built, and foods are made safe and sanitary. Through the method, not only can solar resources be effectively utilized to output clean and green energy, but also efficient planting and breeding can be achieved, by-products are processed into organic fertilizer, and a new path is provided for green agricultural production. Because a full-frame high-standard support system is adopted for the photovoltaic agricultural greenhouse and photovoltaic modules are arranged on the greenhouse, the space of the greenhouse can be effectively utilized, power utilized in the greenhouse can be supplied through a photovoltaic power station, and equivalently, stereoscopic production is formed on the land with the same area.

The invention belongs to the field of plant biotechnology, and in particular relates to a rice thousand kernel weight gene tgw6 mutant as well as a preparation method and application thereof. According to the invention, by designing a specific TGW6 site and by site-directed editing a TGW6 gene for regulating and controlling rice thousand kernel weight by virtue of CRISPR/Cas9 technology, a set of rice tgw6-deleted mutant new germplasm, namely Cas-tgw6-a, Cas-tgw6b or Cas-tgw6c, having an important application value can be obtained, and the mutant can cause significant influence to the thousand kernel weight of rice and can improve rice thousand kernel weight by more than 5%. The tgw6 mutant is applicable to rice high-yield and stable-yield breeding.

The invention relates to a determining method for indicating salt tolerance of different varieties of barleys at seedling stages and belongs to the field of agriculture. The determining method includes adopting a water culturing manner to simulate salt environment artificially, investigating salt damage degree of samples to be tested under the condition of salt stress compared with the blank control condition by measuring seedling height, root length and fresh weight of each seedling of different treatment, and finally determining salt tolerance of the variety. The determining method can be widely applied to screening and evaluation of plant stress resistance genetic resources, particularly used for screening of barley salt-tolerance variety resources, salt-tolerance QTL (quantitative trait locus) genetic analysis, salt-tolerance gene positioning and seed selection of salt-tolerance barley varieties. The determining method has the advantages of simplicity, easiness in mastering and reliable results, the whole salt-tolerance determining process is under control and is slightly affected by the environment, requirements for equipment are low so that only one common illumination culturing box is required.

The invention discloses a new gene purE1 which has high degradability on quinoline. Experiment shows that by using the purE1 gene to transform a microbe, the microbe can obtain degradation activity on quinoline, and the maximal degradation concentration of the degradation activity of the microbe provided by the purE1 gene and its coded protein is up to 850 mg/L. The invention has very important practical application value for the treatment of quinoline contaminants.

The invention discloses a compiling vector of a kiwi fruit gene AcPDS based on CRISPR-Cas9 as well as a construction method and application thereof. The compiling vector of the kiwi fruit gene AcPDS based on CRISPR-Cas9 established can quickly and simply perform efficient site-specific mutagenesis on the kiwi fruit gene, so that the blank of a gene fixed point compiling technology in kiwi fruit is filled. Experimental results verify that by means of agrobacterium-mediated kiwi fruit genetic transformation, the compiling vector of the kiwi fruit gene AcPDS based on CRISPR-Cas9 performs site-specific mutagenesis on two target spots of the kiwi fruit gene AcPDS successfully, and meanwhile, an albefactedphenotype is induced.

The invention discloses a Chinese wolfberry picking machine which comprises a transmission mechanism and a main frame body (3), wherein the main frame body (3) is a door-shaped frame, the lower part of which is provided with a vertical channel, a picking frame and a shaking mechanism are arranged on the upper part of the main frame body (3), a Chinese wolfberry collecting mechanism is arranged on the lower part of the vertical channel of the main frame body (3), and subsequently, a horizontal conveying mechanism (17), a lifting mechanism (13) and a collecting box (12) are arranged in sequence. Compared with the prior art, by using a multipoint evenly-distributed shaking type full-automatic picking mechanism, the picking efficiency is greatly increased, the complete picking rate of fresh fruits of Chinese wolfberry is high, only ripe fruits are harvested in an operating process, and damage cannot be caused to unripe fruits. Through actual calculation, the damage rate to the fruits is not larger than 5% in summer and not larger than 8% in autumn, the complete picking rate is more than 85%, and the working efficiency of each machine can reach 1 to 3 tons every day and is equivalent to more than 30 times that of artificial picking.

The invention discloses a selenium-enriched pleurotus eryngii planting method and a culture medium, comprising the following steps of confecting the culture medium, bagging, sterilizing, inoculating, fungus growing, bud hastening, producing mushroom and harvesting. The materials of the culture medium is manufactured according the weight portions of 70 to 90 portions of cotton seed shell, 8 to 10 portions of bran, 2 to 3 portions of corn meal, 0.6 to 1.2 portions of sucrose, 0.6 to 1.2 portions of gypsum, 4.5 to 6.5 portions of lime, 100 to 140 portions of water and 0.0011 to 0.0014 portion of sodium selenite. The method of the invention domesticates and cultivates the pleurotus eryngii seeds capability of absorbing and enriching selenium to ensure that the selenium-enriched pleurotus eryngii achieves the balanced production and is on the market all year, which improves the output and increases the economic benefit.

The invention relates to a fertilizing device for planting forage grass in the animal husbandry, in particular to a high-efficiency fertilizing device for planting forage grass in the animal husbandry. The invention aims at providing the high-efficiency fertilizing device for planting the forage grass in the animal husbandry, which is high in fertilizing efficiency and convenient in operation and is time-saving and labor-saving. In order to achieve the aim, the invention provides the high-efficiency fertilizing device for planting the forage grass in the animal husbandry. The high-efficiency fertilizing device comprises a bottom plate, wheels, a first guide sleeve, a bracket, a special-shaped rack, a second guide sleeve, a first rack, a first gear, a left frame, a drawing wire, a swinging rod, a spring, a sector-shaped gear, a connecting rod, a first motor, a rotary disc, a clamping block, a connecting rod, a first supporting rod, a piston, a cylinder body, an installing plate, a fixed rod, a fixed plate and a spraying nozzle and the like; the wheels are arranged at the bottom part of the bottom plate in a manner of bilateral symmetry. The fertilizing device provided by the invention has the advantages that the fertilizing efficiency is high, the operation is convenient, and the time and the labor are saved; the fertilizing device plays an important role all the time, not only has good fertilizing effect, but also improve the working efficiency.

The invention relates to a rice salt-tolerant gene OsRR22 mutant, an encoded amino acid sequence thereof, a plant and a making method of the mutant, and belongs to the technical field of plant biology. A CRISPR/Cas9 technology is sued to edit self-selected rice variety WDR58 with rice salt-tolerant gene OsRR22 to obtain a rice salt-tolerant gene OsRR22 function deleted mutant new germplasm WDR58-cas-1 with important application values. The mutant can significantly increase the salt tolerance of rice, and can be applied to high-yielding salt-tolerant rice breeding.

The invention provides a dairy cow feed which can improve the production performance of dairy cows. The dairy cow feed comprises a feed carrier, rumen corrosion inhibitors, microorganism feed additives, sugarbeet, mineral substance additives, vitamin additives, enzyme additives, and Chinese medicine composition additives. According to the dairy cow feed for improving the production performance of dairy cows, by adding the Chinese medicine feed additives, the immune of the dairy cow is improved, so that the disease resistance and the survival rate of the dairy cow are improved, the production performance is improved, and meanwhile the milk performance of the dairy cow and the milk quality are improved, so that milk of good quality can be obtained.

The invention provides an automatic seedling taking manipulator for a plug seedling transplanter. The automatic seedling taking manipulator comprises a seedling taking manipulator and a seedling taking end effector (7), the integral structure is a Cartesian motion system, the seedling taking end effector (7) is driven by a motor (4-7) to shift back and forth along an X-axis in an X-Y plane to align with a hole to be prepared to take a seedling and align with a flowerpot to be prepared to plant the seedling, different air suction and air exhaust modes of the upper end and the lower end of a rodless cylinder (5) drive the seedling taking end effector (7) to slide up and down along a Y-axis in the X-Y plane to take the seedling from a hole dish (1) and plant the seedling in the flowerpot (8). The automatic seedling taking manipulator can replace hand labor of a man, the seedling is taken out from a hole of a source dish and transplanted in a target growing dish or the flowerpot, the integral structure is simple, the performance is reliable, and the automatic seedling taking manipulator is applicable to automatically transplanting the plug seedlings of different specifications.

The invention relates to a lossless testing device for nitrogen nutritive index of crop leaves based on canopy reflection spectrum. It belongs to technology field of crop production, which is specially used in online detecting and precious fertilizing guidance of crop field production. It is mainly composed of bracket, spectrum signal collection and host computer. Spectrum signal collection adopts design of 4 waveband and 8 channels. Hardware system is composed of analog signals conditioning module, A/D transfer module, chip module, memory module, display module, keyboard module, communication module and power management module. Software system is programmed by C51 chip language. Detection of nitrogen content, nitrogen accumulation and leaf area index of canopy not only ensures high detecting precision, but also provides three data indexes of different facets to analyze nitrogen nutritive level of crop, which guarantees reliability and stability of detecting results. It can be applied in field operation, online lossless detecting, field online manage and guidance for agricultural production personnel.

The present invention discloses a method for monitoring growth of farmland crops based on a drone. The method for monitoring the growth of the farmland crops mainly completes the operation of monitoring the growth of the crops through orientation cruise of the drone and fixed point shooting of the imaging device carried by the drone. The present invention saves a large amount of costs for recruiting and using the work force and time through combining the drone remote sensing system with the modern agricultural detecting techniques without observing growth conditions of the crops in the farmland by the farmer regarding large scale integration farmlands in the modern agriculture. Meanwhile, it can be convenient to check and compare the image data recorded by the computer at any time, thereby more accurately analyzing actual growth conditions of the crops in the farmland, and facilitating unified planning and management on the farmland.

The invention provides a production method for Fbxo40 gene knockout pigs. The production method comprises the steps that a CRISPR-Cas9 targeting vector and a PGK-Neo resistant gene are jointly transfected into a porcine embryonic fibroblast to obtain a G418-resistant positive monoclonal cell, an Fbxo40 gene in the positive monoclonal cell generates insertion or deletion mutation, a reading frame generates frame shift and stops in advance, then the obtained cell is cloned to serve as a donor cell for nuclear transfer, and an oocyte serves as a receptor oocyte for nuclear transfer; cloned embryos are obtained through a somatic cell nuclear transfer technique, the high-quality cloned embryos are transferred into the fallopian tube of an oestrous sow, and the Fbxo40 gene knockout pigs are obtained through whole-period development. According to the production method, the Fbxo40 knockout pigs are efficiently obtained through a CRISPR-Cas9 gene editing technique at the low cost, and animal models are supplied to research of muscle development and diseases related to the muscles.

The invention discloses a method for performing target knockout on paddy rice dwarfing gene SD1 by using CRISPR/Cas9 technology. The method comprises the following steps: according to the design principle of CRISPR/Cas9, determining target sites, edited by a CRISPR/Cas9 system, in a paddy rice dwarfing gene SD1 encoding region, designing a primer according to the sequence of the target sites, constructing a CRISPR/Cas9 carrier, converting paddy rice callus tissues by using an agrobacterium tumefaciens mediated method, and screening and identifying to finally obtain a SD1-mutated dwarfing paddy rice strain without transgenic DNA segments. The method is applied to breeding of the dwarfing paddy rice strain, so that the hybridization breeding work can be omitted, and the breeding period of the dwarfing paddy rice strain can be greatly shortened.

The invention relates to a simplified fertilizer application method of wheat and corn crop rotation anniversary, which is changed into an anniversary fertilizer application method that fertilizer application is carried out twice in a wheat season and fertilizer application is carried out once in a corn season from a traditional habitual fertilizer application method that the fertilizer application is carried out twice or three times in a wheat season and fertilizer application is carried out more than twice in a corn season. Wheat slow release fertilizer and corn slow release fertilizer are applied on crops, which not only can plan and consider the nutrient requirement of the anniversary of two-crop crops and ensure dependable crop and increasing yield of the crops but also can simplify fertilizer application times to a great extend and save labor force.

A method for quickly improving the variety of paddy rice to increase the content of moderate straight-chain starch and maltha in paddy rice by molecular marker aided selection includes hybridizing, backcross, quality identification, and molecular marker detection. Its advantages are short period (5 generations) and no change of other characteristics.