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35116 results about "Agricultural science" patented technology

Agricultural science is a broad multidisciplinary field of biology that encompasses the parts of exact, natural, economic and social sciences that are used in the practice and understanding of agriculture. (Veterinary science, but not animal science, is often excluded from the definition.)

Special biological fish-fertilizer for aquaculture and preparation method thereof

The invention discloses a special biological fish fertilizer and a preparation method for the fertilizer used in aquiculture; wherein, the biological fish fertilizer mainly comprises raw materials as follows: microbiological preparation, inorganic fertilizers, fermentation organics, micro-mineral amino acid chelate, mineral composites, and water quality improver. The preparation method for the special biological fish fertilizer in aquiculture comprises steps as follows: microbiological preparation is produced; fermentation organics is then produced, and the micro-mineral amino acid chelate is produced, eventually the finished products are made. The special biological fish fertilizer has the advantages of limiting the growth and reproduction of the harmful algae, promoting the large-scale growth and reproduction of the suitable food for fish, effectively improving the water quality and purifying the water environment, strengthening the fish immunity, reducing the diseases, reducing cost due to the small use level, convenient use, mild odor, no pollution to the water, and environment protection. By combining the modern bioengineering technique, low-temperature drying technique, spray-drying technique, and super-fine crushing techniques, the preparation method has the advantages of stable quality for the fish fertilizers and lower overall cost.
Owner:WUHAN KEYANG BIOTECH

Bio-organic fertilizer produced by using livestock manure

The invention provides an efficient active bio-organic fertilizer produced by using livestock manure and a preparation method thereof. The preparation method comprises the following steps: pre-treating the livestock manure, straw and peat; mixing the livestock manure, straw and peat at a weight ratio of (3-5):(1-2):(1-2); adding a composite ageing bactericide based on the condition that the weight ratio of the mixture to the high-concentrated organic material ageing bactericide is (5000-10000):(1-2), evenly mixing, and then carrying out composting fermentation for 7-10 days, wherein during the composting fermentation, the material needs to be stirred 1-2 times each day; and mixing the material decayed by fermentation with a functional composite bactericide which is generated by single fermentation at a weight ratio of (5000-1000):(1-2), granulating and drying at low temperature so as to obtain the high-efficiency and active bio-organic fertilizer. The process is relatively simple, is easy to operate, and can be used for lowering the product cost is low, saving energy and turning wastes into wealth, thereby being beneficial to promotion of benign ecological circulation and environment conservation; and by utilizing the fertilizer, the quality of the product is good, thereby obviously improving the yield and quality of agricultural products.
Owner:FOUR FRIENDS OF CHENGDU CHEM IND

CAR-T transgene vector based on replication defective recombinant lentivirus and construction method and application of CAR-T transgene vector

The invention discloses a CAR-T transgene vector based on replication defective recombinant lentivirus. The CAR-T transgene vector comprises an original nuclear replicon pUCOri sequence, a resistance gene AmpR sequence containing ampicillin, a virus replicon SV40 Ori sequence, a lentivirus packaging cis element, ZsGreen1 green fluorescent protein, an IRES ribosome binding sequence, a human EF1 alpha promoter , a chimeric antigen receptor of second-generation CAR or third-generation CAR and a regulating element, wherein the original nuclear replicon pUCOri sequence is used for plasmid replication; the resistance gene AmpR sequence is used for massively proliferating target strains; the virus replicon SV40 Ori sequence is used for enhancing replication in eukaryocyte; the lentivirus packaging cis element is used for lentivirus packaging; the ZsGreen1 green fluorescent protein is used for expressing green fluorescent for eukaryocyte; the IRES ribosome binding sequence is used for jointly transcribing and expressing protein; the human EF1 alpha promoter is used for conducting eukaryotic transcription on antigen receptor genes; the chimeric antigen receptor is used for forming the second-generation CAR or the third-generation CAR integrating recognition, transfer and start; the regulating element is used for enhancing expression efficiency of transgenes and used after eWPRE-enhanced type woodchuck hepatitis b virus is transcribed. Besides, the invention further discloses a construction method and application of the vector. By means of the CAR-T transgene vector and the construction method and application of the vector, secretion of cell factors and an in vitro killing effect of CAR-T cells can be remarkably improved, and the clinical treatment effect is remarkable.
Owner:SHANGHAI UNICAR THERAPY BIOPHARM TECH CO LTD

Method for obtaining temperature-sensitive sterile line by performing site-specific mutagenesis on P/TMS12-1 through CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 system

The invention discloses a method for obtaining a temperature-sensitive sterile line by performing site-specific mutagenesis on P/TMS12-1 through a CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 system. The method comprises the following steps: cloning and controlling a Pei'ai 64S temperature-sensitive sterile major gene P/TMS12-1 fragment; designing a target sequence according to the P/TMS12-1 sequence; constructing a pU3-gRNA carrier of the target-containing sequence fragment; constructing a pCRISPR/Cas9 carrier; obtaining a positive transgenic seedling by utilizing the pCRISPR/Cas9 carrier containing the target sequence fragment; screening a mutant plant from the positive transgenic seeding; performing subculture planting on the mutant plant to obtain the temperature-sensitive sterile line without transgenic components. According to the method disclosed by the invention, the CRISPR/Cas9 system is utilized to completely inactivate P/TMS12-1 non-coding RNA, and the temperature-sensitive sterile line without transgenic components is artificially cultivated. The method disclosed by the invention has the advantages of being strong in purposiveness, small in genome damages and capable of avoiding transgenic interference.
Owner:SOUTH CHINA AGRI UNIV

Planting method of Chinese trichosanthes

The invention relates to a planting method of Chinese trichosanthes, belonging to the technical field of plantation and comprising the steps of: variety breeding, seedling reproduction, transplanting, field management, disease and pest control, harvesting and processing, wherein the variety breeding step includes the sub-steps of the tests in different production places, amplification and verification tests, resistance test, field pest investigation and trichosanthes nematode control; the seedling reproduction step includes the sub-steps of seed reproduction and root reproduction; the transplanting step includes the sub-steps of land selection and preparation, ridging, reproduction and planting, planting method implementation and field management; the land selection and preparation step includes the sub-steps of detection on chemical characteristic of soil, basic fertilizer application and disinfection; the reproduction and planting step includes the sub-steps of seed bud selection, rood cutting and breeding, planting and shed putting up; the planting method implementation step includes the sub-steps of planning density control and staminiferous plant matching; the filed management step includes the sub-steps of intertillagement and weeding, top application, shed putting up, pruning and cold prevention, in particular to intertillagement and weeding, root airing, intercrop, earthing, vine supporting and racking, top application, pruning, flower and fruit protection, chemical control and foliage application; and the disease and pest control step includes the sub-steps of control of diseases and pest and control method implementation.
Owner:BOZHOU HUQIAO PHARMA
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