28 results about "Ampicillin" patented technology

The invention provides a method for synthesizing piperacillin with high selectivity. The method comprises the following steps: taking ampicillin as an initial raw material, under anhydrous and alkaline conditions, carrying out selective reaction on side chain amino of ampicillin and triphosgene to generate an isocyanate intermediate, and then coupling the intermediate with N-ethyl-2, 3-diketopiperazine to directly obtain piperacillin. The key point of the invention is that the generation of by-products such as self-coupling urea is effectively inhibited by utilizing the characteristics of moderate reaction activity and high selectivity of the isocyanate intermediate. The method has the advantages of simple route, high selectivity, few byproducts, high yield and the like, and provides a more economical and efficient way for industrial production of piperacillin.

This invention discloses a method for preparing amorphous Cu-MOFs and their applications, relating to the field of metal-organic framework materials technology. The key technical points are: CuCl2 is used as the copper source and 4,4′-bipyridine as the ligand, and Cu-MOFs are synthesized via a solvothermal method. Transmission electron microscopy shows that the obtained material exhibits a typical micron-scale sheet-like morphology. After etching with ammonia, the sample morphology transforms into rough-surfaced nanospheres with a significantly increased pore size, indicating a local rearrangement of its framework structure. X-ray diffraction analysis shows that the original Cu-MOF has high crystallinity, while the diffraction peaks of the etched aCu-MOF essentially disappear, exhibiting amorphous characteristics. As a signal conversion element in a colorimetric sensor, aCu-MOF can achieve reliable detection of ampicillin under neutral conditions. Using thiram as a cofactor, a highly selective thiram colorimetric sensing system can be constructed.

The application discloses application of tegaserod maleate in preparation of an antibacterial drug synergist, and proves that the tegaserod maleate can produce a synergistic antibacterial effect with a plurality of commonly used antibacterial drugs (including tylosin, tetracycline, enrofloxacin, ampicillin and the like), can significantly enhance the antibacterial activity of the antibacterial drugs on gram-negative bacteria, and reduce the drug resistance of bacteria to the antibacterial drugs; the antibacterial composition composed of the tegaserod maleate and the antibacterial drugs can be used for preparing a drug for treating escherichia coli infection, and the synergistic antibacterial mechanism is mainly to destroy the inner and outer membrane permeability of bacteria, regulate the proton motive force of bacteria, inhibit the activity of bacterial efflux pumps, interfere with the energy metabolism homeostasis of bacteria and induce the bacteria to produce oxidative stress. The application provides a new technical strategy for the treatment of clinical escherichia coli infection, and has important clinical application value and industrialization potential.

The invention relates to the technical field of pharmaceutical preparations, in particular to an ampicillin tablet and a preparation method thereof.The method comprises the steps that medicine raw materials of the ampicillin tablet are mixed, granulated, tableted and coated, the medicine raw material mixture is tableted based on initial pressure, the porosity and aperture uniformity are detected to obtain the actual tableting compactness, and the actual tableting compactness is obtained. Determining whether tabletting and coating are carried out based on the actual tabletting compactness to obtain the ampicillin tablet; determining a coating thickness adjustment value based on a comparison result between the dissolution rate of the actual region and the overall standard dissolution rate interval of the ampicillin tablet, and determining atomization pressure and a spraying angle based on the coating thickness adjustment value and an adjustment value difference value; the adjustment initial pressure is determined based on a comparison result of the verification actual dissolution rate and the overall standard dissolution rate intermediate value. According to the method, the actual dissolution rate of the ampicillin tablet is accurately controlled by adjusting the atomization parameters and the initial pressure according to the detection result of the regional dissolution rate, so that the drug effect is uniformly released, and the drug effect exerting reliability is improved.

The application discloses a fermentation method for producing thiamine pyrophosphokinase and belongs to the technical field of bioengineering. The BL21 recombinant engineering bacteria containing pET28a-TPK, pET28a-ProS2-TPK or pET28a-SUMO-TPK are constructed, the seed liquid is prepared, the isopropyl-beta-D-thiogalactoside is used for inducing expression, the bacteria are collected, the cells are broken and the supernatant is collected to obtain the TPK crude enzyme liquid. In the fermentation, the LB / TB culture medium containing 50 μg / mL kanamycin or 100 μg / mL ampicillin is used. The SUMO label is introduced to guide the correct folding of the TPK, so that the soluble expression ratio of the TPK is more than 85%, the ratio is improved by 2 times compared with the common system, the inclusion body ratio is greatly reduced, the process is stable and has good repeatability, and an effective scheme is provided for the industrialized large-scale preparation of the TPK.

The application discloses an application of near-infrared controllable Fe, Zn doped tea polyphenol based carbon dot nanometer enzyme in fruit preservation, and belongs to the technical field of agricultural product preservation.The application takes tea polyphenol as a precursor, and prepares Fe, Zn doped carbon dots (Fe, Zn@GTP) nanometer enzyme with excellent antioxidant and antibacterial activity through a microwave assisted method.The Fe, Zn@GTP nanometer enzyme exhibits near-infrared light enhanced peroxidase, superoxide dismutase and catalase catalytic activity and photo-thermal performance.The Fe, Zn@GTP has antibacterial effects on escherichia coli, staphylococcus aureus, methicillin-resistant staphylococcus aureus, ampicillin-resistant escherichia coli and penicillium expansum, and the inhibition rate of the bacterial biofilm treated by the Fe, Zn@GTP is more than 90%.In the preservation experiment, the PVA / Fe, Zn@GTP film can effectively prolong the preservation period of fruits, significantly reduce the occurrence rate of fruit mold and browning, and the deterioration degree of quality indexes.

This invention belongs to the field of pharmaceutical technology, specifically relating to the application of glutamine in the preparation of drugs that inhibit gene mutations in *E. coli*. This application combines glutamine and ampicillin, and after multiple passages, it can inhibit drug-resistance gene mutations in *E. coli*, significantly reducing the mutation probability of drug-resistance genes, decreasing the fold increase in the minimum inhibitory concentration (MIC) of ampicillin after passage, and lowering the survival rate. Animal experiments have also demonstrated that passaged bacteria with added glutamine and ampicillin are more easily cleared by the body, achieving the effect of slowing down the formation of drug-resistance gene mutations in *E. coli* to ampicillin.

The application discloses a glucose oxidase mutant with simultaneously improved thermal stability and acid resistance and antibacterial application thereof, and belongs to the field of genetic engineering and protein engineering. The application takes glucose oxidase AiGODL as a mother to obtain a mutant AiGODL_E148K / F283Y by respectively mutating Glu148 and Phe283 into Lys148 and Tyr283. It is found through experiments that, compared with wild-type glucose oxidase AiGODL, the mutant AiGODL_E148K / F283Y has improved heat resistance and acid resistance, and has the same level of specific activity and catalytic efficiency as AiGODL and the same antibacterial performance as ampicillin. The mutant AiGODL_E148K / F283Y provided by the application has great application prospect in antibacterial and antibiotic substitution.

The application discloses a pSY1A plasmid vector for a wheat germ cell-free expression system, which is constructed by introducing an ampicillin resistance gene, an Ori replicon, a T7 promoter, a translation enhancer, a MCS (multiple cloning site), a 3'-UTR sequence and a T7 terminator sequence on the basis of part of a sequence of an E. coli plasmid pUC19. The pSY1A plasmid vector has a MCS into which an exogenous gene can be inserted, and the MCS and a transcription assembly constitute a transcription module, so that the transcription module can be preserved by preserving the plasmid, and in-vitro transcription of the wheat germ cell-free expression system becomes more convenient and faster.

This invention discloses the application of inulin in enhancing the efficacy of ampicillin against drug-resistant Salmonella, belonging to the field of pharmaceutical biotechnology. The research of this invention shows that inulin itself does not have an inhibitory effect on Salmonella, but when used in combination with ampicillin, it can significantly enhance the inhibitory effect of ampicillin on Salmonella. Both in vitro and in vivo experiments of this invention have yielded the same conclusion. This invention can reduce the use of antibiotics and provides a new research direction for the prevention and control of Salmonella.

In the present invention, the influence by flora of the skin on dermatitis is examined by analyzing Tmem79-knockout model mice, and a bacterium having a suppressive effect on dermatitis is identified, and the present invention relates to a pharmaceutical composition derived from such a bacterium, containing as active components one or more substances selected from: (a) a bacterial cell of an ampicillin-sensitive bacterium, or a constituent component of the bacterium; (b) a culture supernatant of an ampicillin-sensitive bacterium, or a purified product from the culture supernatant; (c) an extract of an ampicillin-sensitive bacterium; and (d) a metabolite of an ampicillin-sensitive bacterium.

The invention discloses a pSY1A plasmid vector for a wheat germ cell-free expression system, which is characterized in that on the basis of partial sequence of escherichia coli plasmid pUC19, an ampicillin resistance gene, an Ori replicon, a T7 promoter, a translation enhancer, MCS multiple cloning sites, a 3 '-UTR sequence and a T7 terminator sequence are introduced to construct the plasmid vector pSY1A. According to the pSY1A plasmid vector provided by the invention, the vector has multiple cloning sites into which exogenous genes can be inserted, and the multiple cloning sites and a transcription component form a transcription module, so that the transcription module can be stored by storing plasmids, and the in-vitro transcription of a wheat germ cell-free expression system becomes more convenient and quicker.

Disclosed is a method and a device for determining whether a sample contains ampicillin-resistant (AMP-R) non-typhoid Salmonella (NTS). The method comprises detecting, in the sample, the presence of a combination of certain genes or their gene products, wherein the presence of said combination indicates the sample contains AMP-R NTS.

This invention discloses a method based on Dunaliella salina β-carotene isomerase. DsBCI Construction and application of engineered bacteria. This invention discloses for the first time three Dunaliella salina β-carotene isomerases. DsBCI : DsBCI -1, DsBCI -2, DsBCI -3, the amino acid sequence is shown in SEQ ID NO: 1, SEQ ID NO: 3, and SEQ ID NO: 5, and the base sequence of the gene encoding it is shown in SEQ ID NO: 2, SEQ ID NO: 4, and SEQ ID NO: 6. This invention relates to three β-carotene isomerases from Dunaliella salina. DsBCI Construction and application of [the enzyme / organism]. Among them, β-carotene isomerase... DsBCI The vector, based on pETduet-1 containing the T7 promoter and ampicillin resistance, was co-transformed with the W07 plasmid into *E. coli* BL21(DE3) for expression. Three *Dunaliella salina* β-carotene isomerases were verified by measuring the 9-cis-β-carotene content. DsBCI Function and effectiveness of heterologous expression.

The present application belongs to the technical field of complex enzyme. The present application provides a beta-lactamase complex enzyme capable of degrading various antibiotics, and a fixing method and application thereof. The preparation method of the complex enzyme comprises the following steps: extracting gene fragments of CTX-M type broad-spectrum beta-lactamase and VIM type metal-beta-lactamase from Klebsiella pneumoniae; obtaining the target gene fragments through PCR amplification, and constructing into a basic vector to obtain a recombinant vector; transforming the recombinant vector into host cells for heterologous expression; and through protein induction, SDS-PAGE protein gel electrophoresis and protein purification, the complex enzyme is obtained. The complex enzyme successfully degrades penicillins such as ampicillin, carbapenems such as imipenem, cephalosporins such as cefotaxime and other beta-lactams. The complex enzyme gene is successfully expressed in Escherichia coli, and the optimal fixing condition is obtained. The beta-lactamase complex enzyme is fixed under the optimal fixing condition, and can be repeatedly used.

The application discloses a beta-lactamase and a gene for coding the beta-lactamase, and further discloses an expression cassette, a recombinant carrier, a recombinant cell or a recombinant bacteria, and a construction method of the recombinant bacteria, and further discloses the application of the beta-lactamase, the gene for coding the beta-lactamase, the expression cassette, the recombinant carrier, the recombinant cell or the recombinant bacteria, and the construction method of the recombinant bacteria in removing beta-lactam antibiotic pollution in the environment. The beta-lactamase gene is transformed into escherichia coli, so that the escherichia coli can hydrolyze beta-lactam antibiotics in the environment, including ampicillin, cefazolin, cefotaxime, imipenem and amikacin, and has great application value in eliminating beta-lactam antibiotic pollution in the environment.

The present application relates to natamycin production technical field, specifically to a method for improving natamycin fermentation yield, comprising the following steps: shake flask seed culture: under sterile conditions, brown yellow spore streptomyces spores are coated on agar medium, and placed in a culture box for culture, then the cultured brown yellow spore streptomyces is prepared into spore suspension, and the spore suspension is taken and added into a triangular flask of seed culture medium for culture; fermentation tank fermentation culture: the cultured seed liquid is inoculated into a fermentation culture medium comprising proteose peptone, yeast powder, sodium chloride, magnesium sulfate, glucose, betaine, ampicillin, sodium succinate for stirring culture, and glucose is added during the culture until the fermentation is completed, and natamycin is obtained. The present application significantly improves the yield of brown yellow spore streptomyces producing natamycin by adding betaine, ampicillin and sodium succinate in the fermentation culture medium; the production method is simple to operate and is conducive to large-scale industrialization.

The invention discloses nucleic acid aptamers for specifically recognizing florfenicol, thiamphenicol and chloramphenicol, and the nucleic acid aptamers are base sequences shown as SEQ ID NO.1 or SEQ ID NO.3 in a sequence table, or base sequences with the homology of more than 70% with the SEQ ID NO.1, or base sequences with the homology of more than 40% with the SEQ ID NO.3. The invention further discloses a preparation method of the nucleic acid aptamers for specifically recognizing florfenicol, thiamphenicol and chloramphenicol. Experiments prove that the nucleic acid aptamer can specifically recognize all amide alcohol antibiotics clinically used by veterinarians, and is not interfered by ampicillin and other antibiotics. The compound has wide application in the fields of food safety, environmental safety and the like, can be used for preparing a sensor or a detection kit for specifically recognizing florfenicol, thiamphenicol and chloramphenicol, and has important significance in preliminary screening of amido alcohol antibiotic residues and pollution in large-batch food and environmental samples; and a new recognition element is provided for establishing a rapid, simple, cheap and sensitive florfenicol, thiamphenicol and chloramphenicol detection method at the same time.

The present application relates to a kind of H5N1 nucleoprotein prokaryotic expression vector and the method for its nucleoprotein induced expression, belong to the field of bioengineering, the H5N1 nucleoprotein base sequence optimized by in vitro synthesis in the present application, by enzyme cutting site BamHI and XhoI, the H5N1 nucleoprotein optimized base sequence is constructed into PGEX-6P-1 plasmid and obtains H5N1 nucleoprotein prokaryotic expression vector;After being transformed into BL21 competent bacteria by H5N1 nucleoprotein prokaryotic expression vector, inoculate in LB solid culture medium with ampicillin resistance and culture, pick single bacterial clone and inoculate into LB liquid culture medium with ampicillin resistance and culture, finally, IPTG culture induction is obtained, and the nucleoprotein fusion protein of induced expression is obtained, and the nucleoprotein of H5N1 is well expressed, and the fusion protein is obtained, which provides the basis for studying more significant, more durable immunity and effective H5N1 vaccine.

The invention belongs to the technical field of genetically engineered bacteria, and particularly discloses apramycin genetically engineered bacteria with genetic stability as well as a construction method and application of the apramycin genetically engineered bacteria. The construction method comprises the following steps: by taking a plasmid containing an xylE gene and a tsnR gene as a template, obtaining a linearized vector skeleton without a promoter; the method comprises the following steps: inserting a streptomyces indicus promoter sequence into a linearized vector skeleton to enable the promoter to be located at the upstream of an xylE gene and a tsnR gene, and constructing a double-reporter gene expression vector; transferring the expression vector into streptomyces through conjugation to obtain a conjugate; the genetically stable strain is obtained through ultraviolet mutagenesis in combination with catechol chromogenic screening. The biological value is increased by more than 40% compared with that of an original strain, the value retention rate of 94.5% or above is kept in continuous 10 generations of long-term passage experiments, and a powerful technical support is provided for industrial stable production of anti-mycoplasma active substances.

The invention relates to the field of biological medicine, in particular to a method for detecting plasmid copy number in engineering bacteria by qPCR (quantitative polymerase chain reaction). The method comprises the following steps: constructing standard plasmids, establishing methodology, carrying out methodology verification on the method, and carrying out plasmid copy number detection on the strain by using the method. The standard plasmid comprises a reference gene alaA and to-be-detected genes bla and Kan, the ratio of the reference gene alaA to the to-be-detected genes bla to Kan is 1: 1: 1, and the standard plasmid can be used for detecting the copy number of engineered plasmids containing ampicillin resistance genes or kanamycin resistance genes in engineered strains.

The invention provides an acetic acid culture medium for high-density culture of yarrowia lipolytica and a preparation method of the acetic acid culture medium. The culture medium is prepared from the following components in concentration: 1 to 30 g / L of anhydrous sodium acetate, 0.05 to 1 g / L of histidine proline, 0.5 to 41.40 g / L of ammonium sulfate, 0.5 to 5 g / L of monopotassium phosphate, 0.01 to 0.5 g / L of anhydrous magnesium sulfate, 0.05 to 0.1 g / L of ampicillin and the balance of water. According to the method, the treated histidine proline solution is creatively added into the traditional acetic acid culture medium, so that the acetic acid stress is effectively relieved, and the growth and viable count of the yarrowia lipolytica are remarkably improved.

Provided are a tetragenococcus halophilus with an inhibitory effect on bacillus cereus and a use thereof. The preservation number of the tetragenococcus halophilus is GDMCC 64945. The tetragenococcus halophilus is isolated from fermented bean curd, and colonies of the tetragenococcus halophilus are milky white, small and round on a solid medium; The tetragenococcus halophilus is Gram-positive, and when observed under a microscope, the morphology thereof is mostly diplococci or tetrads, with no chain-like formations. The strain is sensitive to eight antibiotics such as ampicillin, gentamicin and kanamycin, and has no drug resistance gene and virulence gene. A bacterial suspension of the strain has a good inhibitory effect on a bacillus cereus strain isolated from the fermented bean curd, and when the strain is applied to a fermented bean curd fermentation system, the growth of bacillus cereus in the system can be effectively inhibited, and the content of enterotoxin in the system is reduced.

The invention discloses a Rongchang pig source ZH30 strain and application thereof, and belongs to the technical field of agricultural livestock feed additives. The ZH30 strain provided by the invention has bacteriostatic activity on a plurality of common pig pathogenic bacteria such as escherichia coli, staphylococcus aureus, salmonella typhimurium, salmonella pullorum and enterococcus faecalis, is wide in antibacterial spectrum, and is beneficial to reducing the load of pathogenic bacteria in intestinal tracts of pigs. Meanwhile, the ZH30 strain is sensitive to streptomycin, gentamicin and ampicillin, infectious microbe pollution can be conveniently controlled through a conventional antibiotic means in the fermentation and preparation production process, and meanwhile the drug resistance spreading risk is reduced. As a functional strain of a probiotic preparation for pigs, the ZH30 strain disclosed by the invention can improve the intestinal health of pigs and improve the mechanical barrier integrity of the intestinal tracts, has potential application values of reducing the diarrhea risk of piglets and improving the feed utilization rate and the production performance, and is of great significance to the development of animal husbandry and feed industry.

The invention belongs to the technical field of microorganisms, and particularly relates to a Bifidobacterium longum subsp. Longum FMBL B250365 MQ which is preserved in the China Center for Type Culture Collection on September 18, 2025, has the preservation number of CCTCC M 20252060, can utilize oligosaccharides such as fructo-oligosaccharide, galactooligosaccharide, maltooligosaccharide, soybean oligosaccharide and the like, and can be used for preparing a feed additive, a feed additive and a feed additive. Good tolerance to acid and cholate is achieved; the compound is sensitive to ampicillin, streptomycin, tetracycline, chloramphenicol, vancomycin, erythromycin, clindamycin and gentamicin; the drug resistance to kanamycin is realized; the caenorhabditis elegans has antioxidant and bacteriostatic capabilities, and the average life of caenorhabditis elegans is prolonged by 18.65%; the compound can be used for preparing anti-aging and life-prolonging medicines, and has a wide application prospect.

The invention discloses a Lactococcus gasseri HN1 strain and an application of the Lactococcus gasseri HN1 strain. The Lactococcus garviae HN1 strain disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 67511. The HN1 strain can resist acid and bile salt and is good in safety, supernate of the HN1 strain has an obvious bacteriostatic effect on aquatic / zoonosis pathogenic bacteria such as vibrio harveyi, escherichia coli, vibrio anguillarum, aeromonas hydrophila, staphylococcus aureus, vibrio parahaemolyticus and edwardsiella tarda, and the HN1 strain can be prepared into antibacterial drugs and used for preventing and treating related diseases. Besides, the HN1 strain is very sensitive to ampicillin and tetracycline, is insensitive to antibacterial drugs such as rifampicin and the like, and also can be combined with insensitive or drug-resistant antibacterial drugs to better prevent and treat diseases caused by pathogenic bacterium infection.

This invention provides a recombinant plasmid for expressing the Bacillus subtilis resistance marker gene without antibiotic resistance, its construction method, and its application. This invention utilizes Bacillus subtilis SCK6 supercompetent cells to directly construct a Bsggt recombinant expression plasmid that does not contain the Ori replication origin site of Escherichia coli or the ampicillin resistance gene. This results in a smaller free plasmid expression system, providing a stable expression tool for heterologous proteins in production applications. The use of Cas9n-AID base editing technology in this invention simplifies the strain screening process. The constructed resistance marker-free expression plasmid is smaller than traditional shuttle plasmids, and no inducing agent is needed during expression. Because the expression plasmid is free of antibiotic resistance markers, the growth rate is significantly increased during culture due to the absence of antibiotic pressure. High expression without antibiotic introduction is more suitable for food safety-grade production applications.