46 results about "Ampicillin" patented technology

The invention discloses a prebiotic composition capable of proliferating intestinal beneficial flora and a preparation method thereof, and the prebiotic composition comprises the following raw materials in parts by weight: 35-45 parts of partially hydrolyzed complex gum, 30-40 parts of green banana resistant starch, 15-25 parts of enzymolysis okra polysaccharide and 3-5 parts of tea polyphenol. According to the composition, the partially hydrolyzed complex gum with specific molecular weight, the green banana resistant starch, the enzymolysis okra polysaccharide and the tea polyphenol are scientifically compounded, so that efficient proliferation of intestinal effective microbial communities and comprehensive and positive regulation and control of intestinal microenvironment are realized, and the composition is prepared by utilizing four components with complementary functions and different effects. The bacillus amyloliquefaciens can effectively proliferate traditional beneficial bacteria such as bifidobacteria and lactobacillus, can effectively nourish butyric acid producing bacteria such as clostridium praeparatum and inhibit growth of harmful bacteria, so that the bacillus amyloliquefaciens has the function of remarkably improving intestinal flora disorder caused by ampicillin, and has the functions of promoting generation of short-chain fatty acid and maintaining intestinal barriers.

The invention provides a method for synthesizing piperacillin with high selectivity. The method comprises the following steps: taking ampicillin as an initial raw material, under anhydrous and alkaline conditions, carrying out selective reaction on side chain amino of ampicillin and triphosgene to generate an isocyanate intermediate, and then coupling the intermediate with N-ethyl-2, 3-diketopiperazine to directly obtain piperacillin. The key point of the invention is that the generation of by-products such as self-coupling urea is effectively inhibited by utilizing the characteristics of moderate reaction activity and high selectivity of the isocyanate intermediate. The method has the advantages of simple route, high selectivity, few byproducts, high yield and the like, and provides a more economical and efficient way for industrial production of piperacillin.

This invention discloses a method for preparing amorphous Cu-MOFs and their applications, relating to the field of metal-organic framework materials technology. The key technical points are: CuCl2 is used as the copper source and 4,4′-bipyridine as the ligand, and Cu-MOFs are synthesized via a solvothermal method. Transmission electron microscopy shows that the obtained material exhibits a typical micron-scale sheet-like morphology. After etching with ammonia, the sample morphology transforms into rough-surfaced nanospheres with a significantly increased pore size, indicating a local rearrangement of its framework structure. X-ray diffraction analysis shows that the original Cu-MOF has high crystallinity, while the diffraction peaks of the etched aCu-MOF essentially disappear, exhibiting amorphous characteristics. As a signal conversion element in a colorimetric sensor, aCu-MOF can achieve reliable detection of ampicillin under neutral conditions. Using thiram as a cofactor, a highly selective thiram colorimetric sensing system can be constructed.

The application discloses application of tegaserod maleate in preparation of an antibacterial drug synergist, and proves that the tegaserod maleate can produce a synergistic antibacterial effect with a plurality of commonly used antibacterial drugs (including tylosin, tetracycline, enrofloxacin, ampicillin and the like), can significantly enhance the antibacterial activity of the antibacterial drugs on gram-negative bacteria, and reduce the drug resistance of bacteria to the antibacterial drugs; the antibacterial composition composed of the tegaserod maleate and the antibacterial drugs can be used for preparing a drug for treating escherichia coli infection, and the synergistic antibacterial mechanism is mainly to destroy the inner and outer membrane permeability of bacteria, regulate the proton motive force of bacteria, inhibit the activity of bacterial efflux pumps, interfere with the energy metabolism homeostasis of bacteria and induce the bacteria to produce oxidative stress. The application provides a new technical strategy for the treatment of clinical escherichia coli infection, and has important clinical application value and industrialization potential.

The invention relates to the technical field of pharmaceutical preparations, in particular to an ampicillin tablet and a preparation method thereof.The method comprises the steps that medicine raw materials of the ampicillin tablet are mixed, granulated, tableted and coated, the medicine raw material mixture is tableted based on initial pressure, the porosity and aperture uniformity are detected to obtain the actual tableting compactness, and the actual tableting compactness is obtained. Determining whether tabletting and coating are carried out based on the actual tabletting compactness to obtain the ampicillin tablet; determining a coating thickness adjustment value based on a comparison result between the dissolution rate of the actual region and the overall standard dissolution rate interval of the ampicillin tablet, and determining atomization pressure and a spraying angle based on the coating thickness adjustment value and an adjustment value difference value; the adjustment initial pressure is determined based on a comparison result of the verification actual dissolution rate and the overall standard dissolution rate intermediate value. According to the method, the actual dissolution rate of the ampicillin tablet is accurately controlled by adjusting the atomization parameters and the initial pressure according to the detection result of the regional dissolution rate, so that the drug effect is uniformly released, and the drug effect exerting reliability is improved.

The application discloses a fermentation method for producing thiamine pyrophosphokinase and belongs to the technical field of bioengineering. The BL21 recombinant engineering bacteria containing pET28a-TPK, pET28a-ProS2-TPK or pET28a-SUMO-TPK are constructed, the seed liquid is prepared, the isopropyl-beta-D-thiogalactoside is used for inducing expression, the bacteria are collected, the cells are broken and the supernatant is collected to obtain the TPK crude enzyme liquid. In the fermentation, the LB / TB culture medium containing 50 μg / mL kanamycin or 100 μg / mL ampicillin is used. The SUMO label is introduced to guide the correct folding of the TPK, so that the soluble expression ratio of the TPK is more than 85%, the ratio is improved by 2 times compared with the common system, the inclusion body ratio is greatly reduced, the process is stable and has good repeatability, and an effective scheme is provided for the industrialized large-scale preparation of the TPK.

The invention discloses a nano-enzyme antibiotic adjuvant as well as a preparation method and application thereof, and belongs to the technical field of nano-drug delivery system antibiosis. The method comprises the following steps: dispersing bovine serum albumin and heme in water, and carrying out ultrasonic oscillation; injecting absolute ethyl alcohol, adding glutaraldehyde, continuing the reaction, centrifuging after the reaction is finished, and collecting a reaction product; and washing the reaction product, carrying out first freeze drying, mixing the product obtained by freeze drying with ampicillin antibiotic, carrying out ultrasonic oscillation, centrifuging, and carrying out second freeze drying to obtain the bovine serum albumin-heme-ampicillin nano-composite. According to the invention, the bovine serum albumin coated heme nano-composite is obtained by a solvent removal method and is embedded with the ampicillin antibiotic, so that good biocompatibility is achieved, and the sterilization efficiency of the ampicillin antibiotic on drug-resistant bacteria is improved.

The application discloses an application of near-infrared controllable Fe, Zn doped tea polyphenol based carbon dot nanometer enzyme in fruit preservation, and belongs to the technical field of agricultural product preservation.The application takes tea polyphenol as a precursor, and prepares Fe, Zn doped carbon dots (Fe, Zn@GTP) nanometer enzyme with excellent antioxidant and antibacterial activity through a microwave assisted method.The Fe, Zn@GTP nanometer enzyme exhibits near-infrared light enhanced peroxidase, superoxide dismutase and catalase catalytic activity and photo-thermal performance.The Fe, Zn@GTP has antibacterial effects on escherichia coli, staphylococcus aureus, methicillin-resistant staphylococcus aureus, ampicillin-resistant escherichia coli and penicillium expansum, and the inhibition rate of the bacterial biofilm treated by the Fe, Zn@GTP is more than 90%.In the preservation experiment, the PVA / Fe, Zn@GTP film can effectively prolong the preservation period of fruits, significantly reduce the occurrence rate of fruit mold and browning, and the deterioration degree of quality indexes.

This invention belongs to the field of pharmaceutical technology, specifically relating to the application of glutamine in the preparation of drugs that inhibit gene mutations in *E. coli*. This application combines glutamine and ampicillin, and after multiple passages, it can inhibit drug-resistance gene mutations in *E. coli*, significantly reducing the mutation probability of drug-resistance genes, decreasing the fold increase in the minimum inhibitory concentration (MIC) of ampicillin after passage, and lowering the survival rate. Animal experiments have also demonstrated that passaged bacteria with added glutamine and ampicillin are more easily cleared by the body, achieving the effect of slowing down the formation of drug-resistance gene mutations in *E. coli* to ampicillin.

The application discloses a glucose oxidase mutant with simultaneously improved thermal stability and acid resistance and antibacterial application thereof, and belongs to the field of genetic engineering and protein engineering. The application takes glucose oxidase AiGODL as a mother to obtain a mutant AiGODL_E148K / F283Y by respectively mutating Glu148 and Phe283 into Lys148 and Tyr283. It is found through experiments that, compared with wild-type glucose oxidase AiGODL, the mutant AiGODL_E148K / F283Y has improved heat resistance and acid resistance, and has the same level of specific activity and catalytic efficiency as AiGODL and the same antibacterial performance as ampicillin. The mutant AiGODL_E148K / F283Y provided by the application has great application prospect in antibacterial and antibiotic substitution.

The application discloses a pSY1A plasmid vector for a wheat germ cell-free expression system, which is constructed by introducing an ampicillin resistance gene, an Ori replicon, a T7 promoter, a translation enhancer, a MCS (multiple cloning site), a 3'-UTR sequence and a T7 terminator sequence on the basis of part of a sequence of an E. coli plasmid pUC19. The pSY1A plasmid vector has a MCS into which an exogenous gene can be inserted, and the MCS and a transcription assembly constitute a transcription module, so that the transcription module can be preserved by preserving the plasmid, and in-vitro transcription of the wheat germ cell-free expression system becomes more convenient and faster.

This invention discloses the application of inulin in enhancing the efficacy of ampicillin against drug-resistant Salmonella, belonging to the field of pharmaceutical biotechnology. The research of this invention shows that inulin itself does not have an inhibitory effect on Salmonella, but when used in combination with ampicillin, it can significantly enhance the inhibitory effect of ampicillin on Salmonella. Both in vitro and in vivo experiments of this invention have yielded the same conclusion. This invention can reduce the use of antibiotics and provides a new research direction for the prevention and control of Salmonella.

In the present invention, the influence by flora of the skin on dermatitis is examined by analyzing Tmem79-knockout model mice, and a bacterium having a suppressive effect on dermatitis is identified, and the present invention relates to a pharmaceutical composition derived from such a bacterium, containing as active components one or more substances selected from: (a) a bacterial cell of an ampicillin-sensitive bacterium, or a constituent component of the bacterium; (b) a culture supernatant of an ampicillin-sensitive bacterium, or a purified product from the culture supernatant; (c) an extract of an ampicillin-sensitive bacterium; and (d) a metabolite of an ampicillin-sensitive bacterium.

The invention belongs to the technical field of biology, and particularly relates to a bifidobacterium longum subsp. Longum FMBL B241768LS, a microbial inoculum and application of the microbial inoculum in hypoglycemic products, the bifidobacterium longum subsp. Longum FMBL B241768LS is preserved in China Center for Type Culture Collection on October 14, 2024, and the preservation number is CCTCC NO: M 20242173; the inhibitory activity of the compound on dipeptidyl peptidase IV is as high as 79.17%; the compound has a relatively good inhibition effect on the activity of alpha-glucosidase and alpha-amylase; the compound shows drug resistance to ciprofloxacin, ampicillin, penicillin G, clindamycin, kanamycin and polymyxin B; a good inhibition effect is achieved on pathogenic bacteria; the strain can be used for preparing medicines, fermented foods, health-care products and food additives for reducing blood sugar and inhibiting pathogenic bacteria, and has a wide application prospect.

The invention discloses a pSY1A plasmid vector for a wheat germ cell-free expression system, which is characterized in that on the basis of partial sequence of escherichia coli plasmid pUC19, an ampicillin resistance gene, an Ori replicon, a T7 promoter, a translation enhancer, MCS multiple cloning sites, a 3 '-UTR sequence and a T7 terminator sequence are introduced to construct the plasmid vector pSY1A. According to the pSY1A plasmid vector provided by the invention, the vector has multiple cloning sites into which exogenous genes can be inserted, and the multiple cloning sites and a transcription component form a transcription module, so that the transcription module can be stored by storing plasmids, and the in-vitro transcription of a wheat germ cell-free expression system becomes more convenient and quicker.

The invention discloses a bacillus amyloliquefaciens genetically engineered bacterium with high antibiotic resistance as well as a construction method and application thereof, the construction of the engineered bacterium comprises the following steps: bacillus amyloliquefaciens is taken as a starting strain, an RNA (Ribonucleic Acid) molecular chaperonin hfq coding gene is knocked out, and the nucleotide sequence of the RNA molecular chaperonin hfq is shown as SEQ ID NO.1. The RNA molecular chaperonin hfq is obtained through cloning, the tolerance of engineering bacteria to erythromycin and ampicillin is increased by 2.44 times and 3.79 times by knocking out the gene or reducing the expression of the gene, and the enhancement of the antibiotic resistance of bacillus is crucial to the threat of drug-resistant bacteria, the improvement of environmental adaptability and the development of biotechnology application. By strengthening the resistance gene, drug-resistant pathogens can be efficiently inhibited, the antibacterial curative effect can be improved, the metabolic activity can be maintained in a complex antibiotic environment, and the pollution repair and industrial fermentation efficiency can be guaranteed.

Disclosed is a method and a device for determining whether a sample contains ampicillin-resistant (AMP-R) non-typhoid Salmonella (NTS). The method comprises detecting, in the sample, the presence of a combination of certain genes or their gene products, wherein the presence of said combination indicates the sample contains AMP-R NTS.

The application discloses a prebiotic composition for proliferating intestinal beneficial bacteria and a preparation method thereof, and comprises the following raw materials in parts by weight: partially hydrolyzed composite glue 35-45 parts, resistant starch of green banana 30-40 parts, enzymatic okra polysaccharide 15-25 parts, and tea polyphenol 3-5 parts. The composition realizes efficient proliferation of intestinal beneficial bacteria and comprehensive and positive regulation of intestinal microenvironment by scientifically compounding partially hydrolyzed composite glue with a specific molecular weight, resistant starch of green banana, enzymatic okra polysaccharide and tea polyphenol. The four components with complementary functions and different effects can not only more efficiently proliferate traditional beneficial bacteria such as bifidobacterium and lactobacillus, but also effectively nourish butyric acid bacteria such as prasrmanella, and inhibit the growth of harmful bacteria, thereby having the functions of significantly improving intestinal flora disorder caused by ampicillin and promoting the generation of short-chain fatty acids and maintaining the intestinal barrier.

The invention relates to the technical field of biochemical engineering, in particular to a process method for producing ectoine through efficient fermentation. According to the method, a culture medium formula of a base material culture medium and trace elements is developed by optimizing the culture medium, and the base material culture medium comprises glucose, ammonium sulfate, ammonium dihydrogen phosphate, citric acid, potassium sulfate, magnesium sulfate, ferrous sulfate, manganese sulfate, ampicillin and streptomycin; the microelements comprise vitamin B1, vitamin B6, NiCl2, CuSO4, CoCl2 and NaMoO4, and the culture medium disclosed by the invention can be used for remarkably improving the metabolic activity of thalli and has a positive effect on improving the yield and the conversion rate of ectoine; according to the method, the yield of ectoine is high through the ectoine synthesis strain, the fermentation dissolved oxygen and the rotating speed are controlled in a segmented mode in the fermentation process, the yield of the ectoine synthesis strain reaches 134.29 g / L and is successfully amplified to the industrial scale, the fermentation content of a 5m < 3 > tank reaches 90.13 g / L, and the method has great industrialization value.

This invention discloses a 4-hydroxyphenylacetic acid 3-hydroxylase mutant, its encoding gene, and its applications. The 4-hydroxyphenylacetic acid 3-hydroxylase mutant is one of the following: glutamine at position 212 of the amino acid sequence shown in SEQ ID NO.1 is mutated to alanine, as shown in SEQ ID NO.3; or glutamic acid at position 216 of the amino acid sequence shown in SEQ ID NO.1 is mutated to alanine, as shown in SEQ ID NO.4. The genetically engineered bacteria (Q212A and E216A) of this invention, after shake-flask fermentation in M9Y medium containing 100 μg / mL ampicillin for 24 h, achieved tanshinone yields of 757.18 mg / L and 744.36 mg / L, respectively. This invention demonstrates good substrate specificity, high catalytic efficiency, simple process, and environmental friendliness in tanshinone biosynthesis.

The invention discloses a method for increasing the yield of extracellular vesicles of lactococcus lactis and application of the method, and belongs to the technical field of biology. The lysozyme and the ampicillin are used for weakening a lactococcus lactis peptidoglycan layer, so that the yield of the natural bacterial extracellular vesicles of the lactococcus lactis is increased. The technological process is simple, and is suitable for industrial amplification and standardized production; the vesicle yield is high, the amplification effect and the purification effect are good, and the method can be used for large-scale preparation of vesicles. The lactococcus lactis extracellular vesicles have the adjuvant efficacy of stimulating the maturation of dendritic cells and the tropism of targeting solid tumor enrichment, and can be used as an immunopotentiator or a sending carrier of vaccines and nano-drugs.

This invention discloses a method based on Dunaliella salina β-carotene isomerase. DsBCI Construction and application of engineered bacteria. This invention discloses for the first time three Dunaliella salina β-carotene isomerases. DsBCI : DsBCI -1, DsBCI -2, DsBCI -3, the amino acid sequence is shown in SEQ ID NO: 1, SEQ ID NO: 3, and SEQ ID NO: 5, and the base sequence of the gene encoding it is shown in SEQ ID NO: 2, SEQ ID NO: 4, and SEQ ID NO: 6. This invention relates to three β-carotene isomerases from Dunaliella salina. DsBCI Construction and application of [the enzyme / organism]. Among them, β-carotene isomerase... DsBCI The vector, based on pETduet-1 containing the T7 promoter and ampicillin resistance, was co-transformed with the W07 plasmid into *E. coli* BL21(DE3) for expression. Three *Dunaliella salina* β-carotene isomerases were verified by measuring the 9-cis-β-carotene content. DsBCI Function and effectiveness of heterologous expression.

The invention discloses a selective culture medium and method for screening or separating aeromonas, and relates to the technical field of microbial culture. The selective culture medium comprises a yeast extract, a nitrogen source, a carbon source, sodium citrate, sodium deoxycholate, sodium thiosulfate, ferric citrate, sodium chloride, agar, an acid-base indicator, ampicillin and diaminopteridine. The method can be used for rapidly and efficiently separating the aeromonas, and provides reliable preposition selection for detection or identification of the aeromonas.

The invention discloses a preparation method and application of a photoelectric self-energized biosensor based on Zr-MOF (at) BiOI, and relates to the technical field of electrochemical sensors. According to the invention, Zr-MOF-coated BiOI / PDA is used as a photoanode, Au-coated Cu-In2S3 is used as a photocathode, the self-powered photoelectrochemical biosensor of a dual-photoelectrode system is constructed, and the sensor shows good sensitivity, reproducibility and reliable stability to ampicillin, and shows excellent performance in analysis of drugs, human serum and urine.

The present application belongs to the technical field of complex enzyme. The present application provides a beta-lactamase complex enzyme capable of degrading various antibiotics, and a fixing method and application thereof. The preparation method of the complex enzyme comprises the following steps: extracting gene fragments of CTX-M type broad-spectrum beta-lactamase and VIM type metal-beta-lactamase from Klebsiella pneumoniae; obtaining the target gene fragments through PCR amplification, and constructing into a basic vector to obtain a recombinant vector; transforming the recombinant vector into host cells for heterologous expression; and through protein induction, SDS-PAGE protein gel electrophoresis and protein purification, the complex enzyme is obtained. The complex enzyme successfully degrades penicillins such as ampicillin, carbapenems such as imipenem, cephalosporins such as cefotaxime and other beta-lactams. The complex enzyme gene is successfully expressed in Escherichia coli, and the optimal fixing condition is obtained. The beta-lactamase complex enzyme is fixed under the optimal fixing condition, and can be repeatedly used.

The application discloses a beta-lactamase and a gene for coding the beta-lactamase, and further discloses an expression cassette, a recombinant carrier, a recombinant cell or a recombinant bacteria, and a construction method of the recombinant bacteria, and further discloses the application of the beta-lactamase, the gene for coding the beta-lactamase, the expression cassette, the recombinant carrier, the recombinant cell or the recombinant bacteria, and the construction method of the recombinant bacteria in removing beta-lactam antibiotic pollution in the environment. The beta-lactamase gene is transformed into escherichia coli, so that the escherichia coli can hydrolyze beta-lactam antibiotics in the environment, including ampicillin, cefazolin, cefotaxime, imipenem and amikacin, and has great application value in eliminating beta-lactam antibiotic pollution in the environment.

The present application relates to natamycin production technical field, specifically to a method for improving natamycin fermentation yield, comprising the following steps: shake flask seed culture: under sterile conditions, brown yellow spore streptomyces spores are coated on agar medium, and placed in a culture box for culture, then the cultured brown yellow spore streptomyces is prepared into spore suspension, and the spore suspension is taken and added into a triangular flask of seed culture medium for culture; fermentation tank fermentation culture: the cultured seed liquid is inoculated into a fermentation culture medium comprising proteose peptone, yeast powder, sodium chloride, magnesium sulfate, glucose, betaine, ampicillin, sodium succinate for stirring culture, and glucose is added during the culture until the fermentation is completed, and natamycin is obtained. The present application significantly improves the yield of brown yellow spore streptomyces producing natamycin by adding betaine, ampicillin and sodium succinate in the fermentation culture medium; the production method is simple to operate and is conducive to large-scale industrialization.

The invention discloses nucleic acid aptamers for specifically recognizing florfenicol, thiamphenicol and chloramphenicol, and the nucleic acid aptamers are base sequences shown as SEQ ID NO.1 or SEQ ID NO.3 in a sequence table, or base sequences with the homology of more than 70% with the SEQ ID NO.1, or base sequences with the homology of more than 40% with the SEQ ID NO.3. The invention further discloses a preparation method of the nucleic acid aptamers for specifically recognizing florfenicol, thiamphenicol and chloramphenicol. Experiments prove that the nucleic acid aptamer can specifically recognize all amide alcohol antibiotics clinically used by veterinarians, and is not interfered by ampicillin and other antibiotics. The compound has wide application in the fields of food safety, environmental safety and the like, can be used for preparing a sensor or a detection kit for specifically recognizing florfenicol, thiamphenicol and chloramphenicol, and has important significance in preliminary screening of amido alcohol antibiotic residues and pollution in large-batch food and environmental samples; and a new recognition element is provided for establishing a rapid, simple, cheap and sensitive florfenicol, thiamphenicol and chloramphenicol detection method at the same time.

The invention provides a preparation method of L-phenylglycine methyl ester hydrochloride, and belongs to the technical field of pharmaceutical chemicals. The preparation method comprises the following steps: deoxidizing a reactor, adding methanol and L-phenylglycine, adding triphosgene in batches at the temperature of-5 to 10 DEG C, and heating to 60 to 70 DEG C for esterification reaction after the triphosgene is completely fed; when it is detected that the purity content of the L-phenylglycine methyl ester hydrochloride is 95% or above, the obtained L-phenylglycine methyl ester hydrochloride mother liquor is concentrated under reduced pressure to be dry; and adding pure water for dissolving, adjusting the pH value to 1.0-3.0, adjusting the temperature to below 20 DEG C, decolorizing, degassing and filtering to obtain an aqueous solution of the L-phenylglycine methyl ester hydrochloride. Compared with a thionyl chloride method for preparation, the method has remarkable advantages in the aspects of production storage, waste gas treatment and the like, the production cycle can be greatly shortened compared with a sulfuric acid method, and the method can be directly used for side chain enzymatic synthesis of beta-lactam antibiotics such as ampicillin, cefalexin and cefaclor.