433 results about "Ampicillin" patented technology

The invention discloses a CAR-T transgene vector based on replication defective recombinant lentivirus. The CAR-T transgene vector comprises an original nuclear replicon pUCOri sequence, a resistance gene AmpR sequence containing ampicillin, a virus replicon SV40 Ori sequence, a lentivirus packaging cis element, ZsGreen1 green fluorescent protein, an IRES ribosome binding sequence, a human EF1 alpha promoter , a chimeric antigen receptor of second-generation CAR or third-generation CAR and a regulating element, wherein the original nuclear replicon pUCOri sequence is used for plasmid replication; the resistance gene AmpR sequence is used for massively proliferating target strains; the virus replicon SV40 Ori sequence is used for enhancing replication in eukaryocyte; the lentivirus packaging cis element is used for lentivirus packaging; the ZsGreen1 green fluorescent protein is used for expressing green fluorescent for eukaryocyte; the IRES ribosome binding sequence is used for jointly transcribing and expressing protein; the human EF1 alpha promoter is used for conducting eukaryotic transcription on antigen receptor genes; the chimeric antigen receptor is used for forming the second-generation CAR or the third-generation CAR integrating recognition, transfer and start; the regulating element is used for enhancing expression efficiency of transgenes and used after eWPRE-enhanced type woodchuck hepatitis b virus is transcribed. Besides, the invention further discloses a construction method and application of the vector. By means of the CAR-T transgene vector and the construction method and application of the vector, secretion of cell factors and an in vitro killing effect of CAR-T cells can be remarkably improved, and the clinical treatment effect is remarkable.

The invention pertains to the technical field of biologic degrading for chemical pesticide, and relates to a bacterial strain that can efficiently degrade Pyrethroid, Organophosphorus and vermectins pesticides and the application of the bacterial strain. The bacterial strain is an Acinetobacter sp. JCX22D bacterial strain, the biologic fature of the bacterial strain is Gram's negative, free from flagella, in form of short rod, is of esterase and lipase activity, free from oxidase activity, and can take use glucose and fructose. The bacterial strain is resistant to Ampicillin and low-concentration Spectinomycin and nalectin, and not resistant to rifamide. The degrading efficiency of the bacterial straine is more than 72% for high-efficiency cypermethrin, cyhalothrin, decamethrim, bifenthrin pesticid, is more than 70% for diazinon, parathion-methyl, phoxime and chlorpyrifos, and is 90% for vermectins. The bacterial strain can be used for biologically degrading above pesticides, and biologically recovering or cleaning any water body, soil and agricultural products that are polluted by pesticides.

This invention discloses a method for preparing ampicillin molecularly imprinted polymer microspheres. The method comprises: preparing 3-6% poly(vinyl alcohol) solution, mixing functional monomers, template molecules, initiator, pore-foaming agent and crosslinker, ultrasonicating, adding into poly(vinyl alcohol) 1788 solution, stirring, stirring in 70 deg.C water for 60 min, filtering, ultrasonicating, washing with double distilled water twice, methanol three times and acetone twice (10 min each time), removing the template molecules, and vacuum-drying at 50 deg.C to obtain ampicillin molecularly imprinted polymer microspheres. The method has such advantages as simple process and high efficiency, and can prepare ampicillin molecularly imprinted polymer microspheres with high molecular recognition ability. When used in solid phase extraction, the ampicillin molecularly imprinted polymer microspheres have such advantages as high column efficiency and high selectivity. The ampicillin molecularly imprinted polymer microspheres can be used for selective separation and high-efficiency enrichment of penicillin in food matrix, such as honey.

A Bacillus strain characterized by (i): sensitivity for ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol; (ii) antimicrobial activity against E. coli and Clostridium perfringens; and (iii) a sporulation percentage of at least 80 when measured after 2 days of incubation. The invention further relates to a method for selecting such strains. Many of the identified strains according to the invention are of the species Bacillus amyloliquefaciens. Some of the Bacillus amyloliquefaciens were further identified as Bacillus amyloliquefaciens subsp. amyloliquefaciens whereas others were identified as amyloliquefaciens subsp. plantarum. A Bacillus strain of the invention may be used as a feed additive to animal feed where it has a probiotic effect.

The invention discloses a method, a special kit and test paper strips for detecting beta-lactam antibiotics based on penicillin-binding protein. The receptor kit used for detecting beta-lactam antibiotics comprises: protein represented by SEQ ID NO: 1, enzyme labeled ampicillin and a standard substance solution, wherein the standard substance is penicillin G. The kit and colloidal gold test paper strips provided by the invention have advantages of high sensitivity, high accuracy, high precision, low cost, simple operation, short detection period, simple storage, and long shelf-life. The kit and colloidal gold test paper strips are suitable for various work units. With the kit and colloidal gold test paper strips, simultaneous and rapid detections of large batches of samples can be realized, and on-site high flux rapid detections can be realized. Therefore, the kit, the colloidal gold test paper strips and the method provided by the invention play an important role in the detections of beta-lactam antibiotics.

The invention provides a recombinant vector used for carrying out foreign gene secretory expression in an auxotrophic kluyveromyces marxianus strain as well as a preparation method and application of the recombinant vector. The recombinant vector comprises ampicillin resistance genes, a PKD1 vector, inulase promoters, signal peptides, multiple cloning sites, inulase terminators, nutritional gene promoters and a nutritional gene open reading flame according to the sequence. The constructed recombinant vector used for carrying out foreign gene secretory expression and the preparation method of the recombinant vector can be used for constructing a transformant to achieve secretory expression of foreign genes.

The invention relates to a genetic-engineering antibacterial peptide, as well as a preparation method and application thereof. The preparation method comprises the following steps: according to the characteristics of the amino acid sequence of Magainin and Cecropin P and LL-37 which are antibacterial peptide, a novel antibacterial peptide gene is designed; the sequence of the gene is synthesized and transformed to be expressed in pichia to form antibacterial-peptide gene transformation pichia engineering bacteria, and to be expressed in a fermentor to achieve the effects of high-density cultivation and efficient expression. Fermentation broth is separated and purified to be an antibacterial peptide product which can be applied to feed and food additives and be used as injection. The antibacterial peptide has the advantages of good antibacterial activity to most gram-negative bacteria and gram-positive bacteria, in particular better effects of inhibiting and killing ampicillin-resistant bacteria and kanamycin-resistant bacteria.

The invention relates to a forming method of a self-assembled passivation layer of a signal probe chain substitution-based aptamer electrochemical sensor (SD-EAB) for detecting ampicillin and sulfadimethoxine, and an electrochemical sensor thereof. A preparation method of ampicillin SD-EAB comprises the following steps: hybridizing a capture probe with the terminal modified by a mercapto group with a ferrocene labeled aptamer signal probe complementing with the capture probe, fixing the obtained hybridized probe on a gold electrode through self-assembling and closing the electrode by using OEG6-OMe as a self-assembled passivation layer molecule. The steps of a preparation method of sulfadimethoxine SD-EAB are similar to above steps. The problem of unable detection of ampicillin and sulfadimethoxine of MCH self-assembled passivation layers commonly used in SD-EAB in the prior art is solved in the invention.

The invention pertains to the technical field of bio-treatment of environmental pollutants, particularly relates to a bacterium for degrading o-nitrobenzaldehyde and the applications thereof in bio-treatment of waste water and remediation of soil environmental pollution. The degrading bacterium of o-nitrobenzaldehyde is the strain ND1 of Alcaligenes sp. The bacterium can degrade o-nitrobenzaldehyde with high efficiency under aerobic condition, and utilize albocarbon, p-nitrophenol, o-nitrophenol, ortho-aminophenol, toluene, p-dimethylaminobenzaldehyde, 2, 4-dinitrophenol, para hydroxy benzoic acid, diphenylamine, benzoic acid, xylene and a plurality of other aromatic compounds. The Alcaligenes sp.ND1 is sensitive to five common antibiotics of tetracycline, kanamycin, streptomycin, chloromycetin and ampicillin, thus providing safe guarantees in the application thereof in the bio-treatment of waste water and remediation of soil environmental pollution.

The preparation process of sodium azlocillin includes the following steps: 1. adding triethylamine slowly into water solution of ampicillin at 0-4 deg.c and stirring to clarify the reaction liquid; 2. adding 2-imidazolidinyl ketacyl chloride into the solution at 0-4 deg.c while dropping water solution of Na2CO3 or NaHCO3 to control pH 7.5-8.5 and performing condensation; 3. adding ethyl acetate into the reaction mixture at 0-5 deg.c and regulating pH value to 1.7-1.9; 4. separating to obtain organic phase, and eliminating heat source and impurity to obtain crystallization liquid; and 5. adding ethyl acetate solution of sodium isocaprylate to crystallize, separating and drying to obtain powdered white sodium azlocillin. The process has convenient operation, mild reaction condition, high yield and high product quality.

The invention discloses a method for preparing piperacillin acid. The method comprises the following steps: adding ampicillin, water and a buffer solution with the pH of 6.0-9.0 into a reactor; adding EDPC into the reactor, meanwhile, adding an alkaline regulator to control the pH to be 6.0-9.0, and reacting for 30-60 minutes in the temperature range of 0 to 10 DEG C while carrying out heat preservation; and adding a solvent to crystallize, controlling the crystallizing point to be 15+/-2 DEG C, dropwise adding an acidic regulator to regulate the end pH to be 1.5-2.0, carrying out crystal growing for 1 hour in the temperature range of 0 to 10 DEG C, and then, filtrating, washing and drying crystals, thereby obtaining the piperacillin acid finished product. According to the method, during acylation, water is used as a solvent, and the buffer solution is added, so that synthetic reaction for piperacillin acid is inhibited from going towards a reverse reaction direction, the yield of piperacillin acid is increased, and the purity of the product is improved.

The present invention relates to a novel nitrile glycoside of Formula I named NIAZIRIDIN and to analogues and derivatives thereof. The present invention also relates to a process for the isolation of a novel nitrile glycoside of Formula I below named NIAZIRIDIN and its derivatives and analogues by bioactivity-guided fractionation from the pods of Moringa oleifera. The present invention particularly relates to the bioenhancing activity of the novel nitrile glycoside of Formula I below named NIAZIRIDIN and its derivatives and analogues in enhancing bioactivity of commonly used antibiotics such as rifampicin, tetracycline and ampicillin against Gram (+) and (-) bacteria. The biomolecule also enhances the absorption of drugs, vitamins and nutrients through the gastro-intestinal membrane increasing their bio-availability. Therefore niaziridin can be used in combination therapy with drugs and nutrients resulting in reduced drug associated toxicity, reduced cost and duration of chemotherapy.

The invention relates to an enzyme immune agent box for detecting ampicillin G, which comprises: enzyme mark plate which coats ampicillin G antibody or antigen, enzyme mark material, ampicillin G standard solution, base material color developing solution, compression cleaning liquid, ending solution, compression twin solution and antibody working solution. The invention also discloses a method for applying the detecting method, which comprises: first doing sample front process, then using the agent box to detect, at last analyzing the detected result.

The invention discloses a multimeric protein having the effect of brain targeting, and a preparation method and usage thereof. The method comprises the steps: firstly, expressing cholera toxin B subunit and a fusion protein EGFP-CTA2-TAT of three proteins: an enhanced green fluorescent protein, a cholera toxin A subunit and cell-penetrating peptide in escherichia coli by incompatible double plasmid systems to obtain CTB gene by PCR amplification; cloning the gene segment into a carrier pET-28a to obtain a recombinant plasmid pET-28a-CTB; using wild type CTA2, EGFP and TAT amino acid sequences as templates, inserting 3 enzyme cutting sites and linkers between EGFP and CTA2, and optimizing to obtain codons suitable for expression in escherichia coli; cloning the gene segment into a carrier PET-22b (+) to obtain recombinant plasmid PET-22b-EGFP-CTA2-TAT; using different resistances of PET-28a-CTB and PET-22b-EGFP-CTA2-TAT, and co-transforming the different resistances of the PET-28a-CTB and the PET-22b-EGFP-CTA2-TAT into escherichia coli BL21, to obtain engineering bacteria after screening under double resistance selection pressure of penbritin and kanamycin. CTB5/EGFP-CTA2-TAT chimeric proteins can be obtained after inducible expression of the engineering bacteria by IPTG. The invention further discloses the preparation method and usage of the protein.

The invention relates to reductive chromium ion bacillus thuringiensis YB-03 and a cultivation method and application of the reductive chromium ion bacillus thuringiensis YB-03. According to the invention, the separated reductive chromium ion bacillus thuringiensis YB-03 strain with CGMCC (China General Microbiological Culture Collection Center) NO.5653 not only can reduce Cr<6+>, but also can generate resistance to Ag<+>, Hg<2+>, Cu<2+>, Zn<2+>, Co<2+>, Mn<2+> at different degrees, is insensitive to penbritin and streptomycin and has important application value in treatment of chromium pollution.

The invention provides a recombinant vector for expressing a histidine-tag-fused foreign gene in a Kluyveromyces marxianus nutritional deficient strain, and a preparation method and application of the recombinant vector. The recombinant vector sequentially comprises an ampicillin resistance gene, a PKD1 vector sequence, an inulase promotor, multiple cloning sites, an inulase terminator, a nutritional gene promotor and a nutritional gene open reading frame, and further comprises histidine tags located at C ends or N ends of the multiple cloning sites. The recombinant vector provided by the invention can be used for building a transformant to realize expression of the foreign gene.

The invention discloses a fabrication method of an ampicillin sensor, and belongs to the technical field of novel nanometer functional material and biological sensing analysis. A nickel-iron thermometal layered hydroxide nanosheet array is fabricated on a disposable throwable electrode, an electronic medium-containing polydopamine thin film and a molecular imprinting polymer taking ampicillin as atemplate molecule polymer are directly and sequentially fabricated on the nickel-iron thermometal layered hydroxide nanosheet array by an in-situ growth method according to large specific surface area, high-activity hydroxyl functional group and amino functional group of polydopamine. After the template molecule is eluted, the original position of the template molecule is changed to holes, and the molecule imprinting polymer of the template molecule is eluted. Therefore, the ampicillin sensor is fabricated and completed.

The invention relates to an artificial chromosome transfer vector for recombinant herpesvirus-of-turkey bacteria, and belongs to the field of biological pharmacy. The artificial chromosome transfer vector for the recombinant herpesvirus-of-turkey bacteria is constructed by the following steps of: amplifying a xanthine-guanine phosphotransferase gene (gpt gene) in E.coli DH5alpha chromosome DNA, connecting the xanthine-gpt gene and a CMV promoter in plasmid pEGFP and poly (A), inserting the connected body into plasmid pUAB to construct recombinant clone plasmid pUAB-gpt with Egpt; and preparing a high-copy BAC vector basic functional sequence by using a pUC18 clone single-copy BAC vector, and inserting the high-copy BAC vector basic functional sequence into the pUAB-gpt to obtain the artificial chromosome transfer vector for the recombinant herpesvirus-of-turkey bacteria pUAB-gpt-BAC, wherein the pUAB-gpt-BAC contains a core sequence BAC-gpt of a gpt gene expression box and has double resistances of ampicillin and chloramphenicol. The constructed transfer vector has the application prospect for developing recombinant HVT live virus vector gene engineering vaccine.

The invention relates to a preparation method for Azlocillin sodium, which belongs to the synthetic technical field of antibiotics. The preparation method comprises the following steps: preparing a solution used for a condensation reaction, drawing dichloromethane into a reactor, putting ampicillin into the reactor with stirring, controlling the weight ratio of dichloromethane to ampicillin, thencooling and controlling the reduced temperature, then regulating the pH value to alkalescence by a pH regulator to obtain an solution used for condensation reaction; performing a condensation reaction; extracting and separating; crystallizing; drying; performing a salifying reaction; decoloring; freezing and drying; crushing. The invention has the advantages that the preparation method is capableof simplifying the operation, reducing the usage of the chemical solutions and the risks on environmental pollution; and has simple operation; can effectively avoid the loss caused by chromatography,raise the yield of Azlocillin sodium; and has mild salifying reaction condition, reduce the risk that Azlocillin sodium can be decomposed in alkali. The content of a single impurity of the obtained Azlocillin sodium dried product is lower than 0.6 percent and the content of total impurity of the obtained Azlocillin sodium dried product is lower than 1 percent which are obviously superior to the pharmacopoeia standard.