Genetic engineering antibiotic peptides as well as preparation method and application thereof

A technology of genetic engineering and antimicrobial peptides, applied in the field of bioengineering drug production

Active Publication Date: 2009-06-10
山东华辰制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Separation and purification of fermentatio

Method used

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  • Genetic engineering antibiotic peptides as well as preparation method and application thereof
  • Genetic engineering antibiotic peptides as well as preparation method and application thereof
  • Genetic engineering antibiotic peptides as well as preparation method and application thereof

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Experimental program
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Embodiment Construction

[0008] Technical scheme of the present invention is as follows:

[0009] 1. Genetically engineered antimicrobial peptides

[0010] The genetically engineered antimicrobial peptide of the present invention has the following sequence:

[0011] GCTCTC6

[0012] GAGAAAAGAG GTATTGGTAA GTTTTTGCAT TCTGCTAAGA AGTTTGGTAA GGCTTTTGTT 66

[0013] GGTGAGATTA TGAACTCTGA GAAAAGATCT TGGTTGTCTA AAACTGCTAA AAAATTGGAA 126

[0014] AATTCTGCTA AAAAGCGTAT TTCTGAAGGT ATTGCTATTG CTATTCAAGG TGGTCCACGT 186

[0015] GAGAAAAGAT TGTTGGGTGA CTTCTTCAGA AAGTCCAAGG AAAAGATCGG TAAGGAGTTC 246

[0016] AAGAGAATCG TCCAGAGAAT CAAGGACTTC TTGAGGAACT TGGTCCCAAG AACTGAATCC 306

[0017] CAATGATCTA GAAGT 321

[0018] 2. Preparation of genetically engineered antimicrobial peptides

[0019] The preparation method of the genetic engineering antibacterial peptide of the present invention, the steps are as follows:

[0020] 1) Synthetic antimicrobial peptide g...

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Abstract

The invention relates to a genetic-engineering antibacterial peptide, as well as a preparation method and application thereof. The preparation method comprises the following steps: according to the characteristics of the amino acid sequence of Magainin and Cecropin P and LL-37 which are antibacterial peptide, a novel antibacterial peptide gene is designed; the sequence of the gene is synthesized and transformed to be expressed in pichia to form antibacterial-peptide gene transformation pichia engineering bacteria, and to be expressed in a fermentor to achieve the effects of high-density cultivation and efficient expression. Fermentation broth is separated and purified to be an antibacterial peptide product which can be applied to feed and food additives and be used as injection. The antibacterial peptide has the advantages of good antibacterial activity to most gram-negative bacteria and gram-positive bacteria, in particular better effects of inhibiting and killing ampicillin-resistant bacteria and kanamycin-resistant bacteria.

Description

technical field [0001] The invention relates to the preparation of a novel genetic engineering antibacterial peptide, which belongs to the technical field of bioengineering drug production in modern agricultural technology. Background technique [0002] Antimicrobial peptides are small molecule polypeptides with biological activity induced by organisms, with a molecular weight of about 2000-7000 and composed of 20-60 amino acid residues. Most of these active peptides have the characteristics of strong alkalinity, thermal stability and broad-spectrum antibacterial properties. The first antimicrobial peptide discovered in the world was a polypeptide with antibacterial activity produced by the chrysalis of the silkworm chrysalis, named Cecropins, in 1980 by Swedish scientist G. Boman et al. by injecting Bacillus vaginalis and E. In the following years, people successively discovered and isolated peptides with antibacterial activity from bacteria, fungi, amphibians, insects, hi...

Claims

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Application Information

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IPC IPC(8): C07K14/00C12N15/11C12N15/81C12P21/04A23K1/16A23L1/305A23K20/147A23L33/18
Inventor 姜正军陈溥言刘刚许振龙苏晓明崔丽萍王茂超柳少杰刘璐璐
Owner 山东华辰制药有限公司
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