819 results about "Genetically engineered" patented technology

Methods and systems are provided for generating and utilizing a genetically engineered bacterium comprising a modification in glnD, wherein said modification is selected from the group consisting of: deletion of the entire gene, deletion of substantially the entire gene, deletion of an ACT domain, deletion of more than 50% of an ACT domain, deactivation of an ACT domain, and deactivation of an UTase domain.

The invention relates to fusarium venanii with high protein conversion rate and application of the fusarium venanii to improvement of hypha protein satiety, and belongs to the field of genetic engineering technology and food biotechnology. The fusarium venenatum FCPD disclosed by the invention is preserved in the China Center for Type Culture Collection on December 6, 2024, and the preservation number of the fusarium venenatum FCPD is CCTCC (China Center for Type Culture Collection) NO: M 20242752. The glucose consumption of a substrate for producing mycelia with the same dry weight by the fusarium venanii is reduced by 40-60% compared with that of a wild type, the protein conversion rate is improved by 70-80%, the grease content is improved by 90-110%, the gastric semi-emptying time of in-vitro simulated digestion is prolonged by 10-30%, and the quality of fusarium venanii mycelium protein is remarkably improved. Genetic engineering modification work can be further developed, and fusarium strains with high substrate conversion rate, high grease content and high satiety potential can be cultivated.

The invention discloses an application of IbWRKY26 protein and a coding gene thereof in regulation and control of sweet potato soft rot resistance, and belongs to the technical field of plant genetic engineering. The invention provides an application of the IbWRKY26 protein in any one of the following items: (1) an application in regulation and control of sweet potato soft rot; (2) application in preparation of products for regulating and controlling sweet potato soft rot; (3) application in soft rot resistant sweet potato breeding; (4) application in cultivation of soft rot resistant sweet potatoes; the amino acid sequence of the IbWRKY26 protein is as shown in SEQ ID NO. 2. Experiments prove that compared with a wild type sweet potato, the soft rot disease resistance of the overexpressed IbWRKY26 gene is remarkably improved. The IbWRKY26 protein and the coding gene thereof provided by the invention have important theoretical significance and application value in the research on the soft rot resistance of sweet potatoes.

The invention discloses an effect of Collagen in relieving vomitoxin injury of bovine rumen epithelial cells by vitamin E, and belongs to the technical field of genetic engineering, the Collagen is COL1A1 protein, COL2A1 protein and COL5A1 protein; the amino acid sequence of the COL1A1 protein is as shown in SEQ ID NO. 19; the amino acid sequence of the COL2A1 protein is as shown in SEQ ID NO. 20; the amino acid sequence of the COL5A1 protein is as shown in SEQ ID NO. 21. According to the effect of Collagen in relieving bovine rumen epithelial cells damaged by vomitoxin through vitamin E, it is found that vitamin E can effectively protect the cell membrane integrity of the bovine rumen epithelial cells and can relieve cell viability decline, oxidative stress, immune and inflammatory reactions and the cell apoptosis rate caused by DON; vitamin E can promote ECM receptor effect and protein digestion and absorption ways by adjusting Collagen protein so as to protect rumen epithelial cells of dairy cows.

The present invention relates to a genetically engineered strain of Saccharomyces cerevisiae for the synthesis of 7-DHC exocytosis, and a construction method and application thereof. The present invention constructs a recombinant S.cerevisiae strain sc13 that can efficiently transport 7-DHC to the extracellular space. When it is used for 500mL shake flask biphasic fermentation, the total production of 7-DHC reaches 28.189mg / g (secretion amount 11.701mg / g), compared with the control sc1 strain, the total production of 7-DHC increases by 14.54 times, and the total extracellular secretion increases by 13.77 times, wherein the secretion production of extracellular 7-DHC accounts for 41.51%. The recombinant strain constructed by the present invention has stronger exocrine ability and higher yield, provides a guiding idea for the synthesis of 7-DHC and the simplification of the separation and extraction of 7-DHC, and has broad application prospects.

The present disclosure is in the field of plant genetic engineering. Specifically, the invention relates to application of a mutant GmLn protein and an encoding gene thereof in regulation and control of soybean yield, in particular to but not limited to a method for obtaining soybeans capable of being densely planted, a method for detecting the soybeans capable of being densely planted, related primers and kits, and further relates to soybean plants capable of being densely planted and a method for improving soybean planting density. According to the method provided by the invention, the soybean target sequence is subjected to gene editing by expressing the gene editing tool in the soybean, so that the soybean which is improved in yield and can be densely planted is obtained.

The invention belongs to the technical field of genetic engineering and agricultural biology, and provides application of dsRNA for specifically inhibiting RpHsp22.6 gene expression in preparation of insecticides, and the nucleotide sequence of the dsRNA is shown as SEQ ID NO.12. The invention further provides a preparation method of the dsRNA. The invention also provides a method for relieving soybean blue syndrome and provides a small heat shock protein RpHsp22.6 of Emblycoris lividipennis. According to the invention, the problem of how to more effectively and more environmentally prevent and treat the plant diseases and insect pests of the Adelphocoris indica is solved, and the effect of reducing the pathogenicity of the Adelphocoris indica to the soybeans is achieved.

The invention provides a method for constitutive expression of foreign protein by escherichia coli Nissle 1917, and belongs to the technical field of genetically engineered bacteria. The invention provides a method for constitutive expression of foreign protein by Escherichia coli Nissle 1917 (EcN), which is characterized in that EcN is used as a chassis strain, an engineering strain for constitutive expression of the foreign protein is constructed by transforming a covert plasmid, and high-efficiency expression of the foreign protein can be realized without induction of an inducer and antibiotic pressure. By utilizing the method disclosed by the invention, the EcN engineering bacterium for constitutive expression of the foreign protein RtSOD is successfully constructed, constitutive efficient expression of the high-stability RtSOD protein in the EcN engineering bacterium can be realized without using antibiotic pressure and inducer induction, and the EcN engineering bacterium can be used as a potential viable bacterium medicine for preventing and treating oxidative stress related diseases and has a wide application prospect. And the method has important significance on the practical application of the EcN engineering strain.

The invention belongs to the technical field of plant genetic engineering molecular breeding, and particularly relates to application of wheat TabHLH93 protein or a coding gene thereof in regulation and control of plant grain development. According to the invention, a binary expression vector capable of realizing ectopic expression of the TabHLH93 gene is constructed and is genetically transformed into common wheat to obtain a progeny with high expression of the TabHLH93 gene, so that overexpression of the TabHLH93 gene is realized, and it is verified that the TabHLH93 has a function of regulating and controlling the size of wheat grains and also has an important regulation and control effect on agronomic characters such as wheat plant height, tiller number, spike length and thousand seed weight. A candidate gene with an important application prospect is provided for wheat high-yield genetic improvement, and the gene has important significance for guaranteeing grain safety.

The invention provides a branched chain amino acid transaminase gene and application thereof, and belongs to the field of gene engineering. According to the invention, the branched chain amino acid transaminase gene HvBCAT5 is found for the first time, and the gene has obvious positive correlation with the accumulation of valine (Val), isoleucine (Ile) and leucine (Leu) of crops. The invention provides an important target gene HvBCAT5 for crop quality improvement, and has important value for increasing the content of branched chain amino acid of crops and improving dietary nutrition. The HvBCAT5 gene as well as a recombinant vector, a recombinant bacterium and a transgenic plant thereof have good application prospects in the fields of food and feed industry.

The invention discloses application of an OsCPN10a gene in regulation and control of vigor, grain shape and drought tolerance of rice seeds, and belongs to the technical field of rice gene engineering. The invention particularly relates to the research of the OsCPN10a gene in the aspects of regulating and controlling the germination rate, the germination potential, the germination index, the grain length, the grain width, the thousand grain weight, the drought tolerance of the rice seeds and the like. Experimental results show that the grain shape of the OsCPN10a knockout mutant seed is obviously lengthened and widened, and the grain shape of the OsCPN10a overexpression seed is opposite; the yield of the Ocpn10a mutant is higher than that of a TNG67 plant; overexpression of the OsCPN10a has the potential of improving the drought resistance of the rice. The invention reveals that OsCPN10a affects genetic mechanisms such as rice seed vigor, grain length, grain width and storability, provides important gene resources and theoretical basis for genetic improvement of rice, and is beneficial to cultivation of a new rice variety with better grain shape and higher yield.

The invention provides an efficient fermentation production method and application of acid type sophorolipid, and the production method comprises the following steps: (1) in a growth stage, adding a composite nitrogen source and growth factors into a basic culture medium to promote the growth of zymophyte and realize high-density fermentation, the zymophyte being candida utilis subjected to genetic engineering modification; (2) in the product synthesis stage, mixed syrup and vegetable oil are used as carbon sources, and the concentration of glucose in a fermentation tank is maintained at 1-5g / L and the concentration of residual oil is maintained at 1-5g / L by regulating and controlling the material supplementing rate. The fermentation production method provided by the invention solves the problems of slow thallus growth, long fermentation period, low production intensity, uncontrollable product composition, more byproducts, peculiar smell of fermentation tail gas and the like in the existing sophorolipid production process, and has industrial application value.

The invention discloses application of a peanut AhNF-YC9 gene in improving the oil content and salt tolerance of plants, and belongs to the technical field of gene engineering. The nucleotide sequence of the peanut AhNF-YC9 gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the peanut AhNF-YC9 gene is as shown in SEQ ID NO: 2. The AhNF-YC9 gene is used for constructing a plant expression vector to transform peanuts, so that the AhNF-YC9 gene is excessively expressed in the peanuts, and the oil content of obtained transgenic peanut seeds can be increased by 4.0% at most compared with that of non-transgenic seeds; after stress treatment with 350mM NaCl, the salt tolerance of the transgenic peanut is obviously higher than that of a non-transgenic plant. Therefore, the peanut AhNF-YC9 gene has an important application prospect in improving the oil content and the salt tolerance of the plant.

The invention provides a recombinant chicken antibacterial peptide aiming at drug-resistant staphylococcus aureus, and belongs to the technical field of veterinarians. The recombinant chicken antibacterial peptide is expressed through genetic engineering recombinant escherichia coli. Three natural chicken antibacterial peptide active regions are fused, and two disulfide bonds are introduced to stabilize the structure of the recombinant chicken antibacterial peptide and enhance the affinity of the recombinant chicken antibacterial peptide to staphylococcus aureus outer membrane protein. The minimum inhibitory concentration of the recombinant chicken antibacterial peptide to the drug-resistant staphylococcus aureus is obviously lower than that of three natural chicken antibacterial peptides, and the formation of a biomembrane of a USA300 strain of the drug-resistant staphylococcus aureus can be thoroughly inhibited when the minimum inhibitory concentration is 8 mu g / mL. The recombinant chicken antibacterial peptide has a good antibacterial effect on different drug-resistant staphylococcus aureus separated from a chicken house, and can effectively solve the infection problem of the drug-resistant staphylococcus aureus in the chicken house.

The invention discloses an antigen fusion protein and application thereof in preparation of a live vaccine for preventing infectious bursal disease. Specifically disclosed are antigen fusion proteins comprising a VP2 protein, a trimer tag, and a C3d protein. The invention also discloses recombinant Eimeria heap constructed by using the antigen fusion protein and application of the recombinant Eimeria heap in preparation of IBDV live vaccines. The oocyst of the recombinant Eimeria heap can effectively induce humoral immune response aiming at IBDV as a vaccine active ingredient, the level of an induced antibody is obviously higher than that of an insect strain expressed by a single VP2 antigen, viruses can be more effectively neutralized, the immune protection efficacy is improved, the safety is high, and the oocyst has the potential of serving as a live vector genetic engineering vaccine. The IBDV live vaccine can be orally taken through drinking water or feed, vaccination is simple, large-scale immunization of chicken flocks can be achieved, and the IBDV live vaccine is economical and efficient and has a wide prospect of being applied to prevention and control of the IBDV of the chicken flocks.

The invention relates to the technical field of genetic engineering and biological agents, in particular to a hybrid peptide with immunoregulation and anti-oxidation functions and a preparation method and application of the hybrid peptide. The polypeptide provided by the invention has immunoregulation and antioxidation functions at the same time, and in a normal or immunosuppression state, the polypeptide can significantly improve the immunologic function of a body and reduce the damage of immunosuppression to the body; the oxidation resistance of cells and organisms can be enhanced; meanwhile, the polypeptide has the advantages of low cytotoxicity, high safety, simplicity and convenience in preparation and low cost, can be used as an ideal immunomodulator and an antioxidant, is widely applied to the fields of medicines, foods, health care, feeds, nutrition and the like of human and animals, and has very good application potential and value.

The invention discloses a rice large-scale metabolism regulator DGP1 and crop metabolism quality design. The invention finds that the structural domain of the rice DGP1 family protein TIGR01589 targets a plurality of metabolic enzymes and promotes the metabolic enzymes to be transferred to a cell nucleus; the structural domain also has an intervention effect on a light breathing pathway by changing the composition of a glycine decarboxylase compound in mitochondria. Based on a molecular mechanism of DGP1, a chimeric protein ST containing a conservative structural domain and coupled with a chloroplast positioning signal is designed, and genetic engineering modification of pakchoi is realized. The modification strategy not only can guide substrate flow of a central glycolysis pathway to flavonoid biosynthesis, but also can promote accumulation of saccharides derived from photosynthetic products in leaves.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a soybean low-phosphorus-tolerant gene GmVQ32 in improving low-phosphorus stress tolerance of plants. The soybean low-phosphorus-tolerant gene GmVQ32 disclosed by the invention can be used for enhancing the root growth and phosphorus absorption efficiency of plants by positively regulating the response of plant root systems to low-phosphorus stress, so that the adaptability of the plants to a low-phosphorus environment is improved.

The invention discloses an application of a sweet potato transcription factor IbERF114 and a coding gene thereof in regulation and control of sweet potato soft rot resistance, and belongs to the technical field of gene engineering. Experiments prove that compared with a wild sweet potato plant, a transgenic sweet potato plant over-expressing the IbERF114 gene and an interference sweet potato plant interfering the IbERF114 gene have the advantages that the resistance of the transgenic sweet potato plant to the soft rot is obviously enhanced, the resistance of the interference sweet potato plant is obviously reduced, and the IbERF114 protein and the coding gene thereof play a key regulation role in the soft rot resistance of the sweet potato. The discovery of functions of the protein and the coding gene thereof has important production values for improving the disease resistance of sweet potatoes, creating excellent disease-resistant parent elements and cultivating high-resistance varieties.

The invention relates to the technical field of genetic engineering, in particular to recombinant humanized collagen and a preparation method thereof. The recombinant humanized III-type collagen provided by the invention can be efficiently and stably expressed under three expression systems of yeast, escherichia coli and CHO cells, and has biological activity superior to that of commercially available collagen products. The recombinant humanized III-type collagen disclosed by the invention has structural characteristics and functional activity similar to those of natural collagen, so that the recombinant humanized III-type collagen has wide application prospects in the aspects of biomedical materials, tissue engineering products, cosmetics, foods, health care products or medicines, medical instruments, medical cosmetology and the like.

The application belongs to the technical field of genetic engineering, and discloses a kind of saccharomyces cerevisiae for producing rare ginsenoside by seaweed biomass and a construction method and application thereof.The saccharomyces cerevisiae has the following characteristics: overexpression of agarase, neojuncanohydrolase, hydroxymethylglutaryl coenzyme A reductase, isopentenyl diphosphate delta isomerase, dammaradienol synthase, protopanaxadiol synthase, cytochrome P450 reductase, protopanaxatriol synthase and glycosyltransferase.The application combines enzymolysis of seaweed biomass with fermentation of rare ginsenoside, which not only endows wild-type yeast with the ability to degrade seaweed biomass that it originally does not have, but also effectively improves the yield of squalene and downstream terpenes (rare ginsenoside Rh1) by overexpression of tHMG1 and IDI1.The saccharomyces cerevisiae can effectively utilize seaweed biomass to obtain squalene and rare ginsenoside Rh1, and has the characteristics of simplicity, economy and ecological friendliness, can convert cheap biomass into high-value products, and provides a way for the development of marine bioeconomy.

The invention belongs to the field of plant genetic engineering, and particularly relates to application of an allele of a rice leaf included angle gene OsPUB77. The invention provides application of rice leaf included angle alleles OsPUB77R and OsPUB77I, and the application is at least one of the following applications: regulating and controlling a rice leaf included angle and improving a rice plant type; the nucleotide sequence of the rice leaf included angle allele OsPUB77R is as shown in SEQ ID NO: 1; the nucleotide sequence of the rice leaf included angle allele OsPUB77I is as shown in SEQ ID NO: 3. The rice containing the allele OsPUB77R is small in leaf included angle and compact in plant type; the rice containing the allele OsPUB77I is large in leaf included angle and loose in plant type.

The present invention relates to crop plants, particularly to plants of the Solanaceae family including tomato (Solarium lycopersicum) plants genetically engineered to have reduced expression and / or activity of SnRK2.3 protein and producing higher yield compared to a corresponding crop plant not engineered to have reduced expression and / or activity of the SnRK2.3 protein.

The invention discloses a method for regulating and controlling soft rot resistance of sweet potatoes by utilizing an IbHD2C gene and application of the method, and belongs to the technical field of gene engineering. The invention discloses an application of an IbHD2C gene (the nucleotide sequence is shown as SEQ ID NO.3) and an encoding protein (the amino acid sequence is shown as SEQ ID NO.4) thereof in regulation and control of soft rot resistance. Experiments prove that the resistance of sweet potatoes to soft rot can be remarkably improved by interfering the IbHD2C gene, and the resistance is reduced by overexpressing the gene. The invention provides a new target for disease-resistant breeding of sweet potatoes, solves the problems of dependence on chemical agents and lack of disease-resistant varieties in the prior art, and has the advantages of high efficiency, environmental protection and sustainability.

The invention discloses a method for efficiently obtaining pepper transgenic hairy roots by using agrobacterium rhizogenes. Belongs to the technical field of plant genetic engineering. The method takes zanthoxylum bungeanum stem nodes as receptors, obtains hairy roots by agrobacterium rhizogenes mediation, and comprises the following steps: constructing an expression vector; performing agrobacterium tumefaciens transformation; preparing a transgenic receptor; carrying out explant infection; inducing hairy roots; transplanting the transgenic hairy root plant; and identifying the transgenic hairy roots. The method has the advantages of stable system, high conversion rate, simplicity in operation, workload saving and the like.

The invention discloses a xylanase mutant with synchronously improved thermal stability and pH tolerance and application of the xylanase mutant, and belongs to the field of gene engineering and protein engineering. According to the invention, xylanase AbXyL10A from Agaricus bisporus var burnettii is taken as a female parent, and two amino acid sites of Thr234 and Arg268 are subjected to mutation, so that two types of xylanase mutants T234N and R268L are obtained. Compared with wild type xylanase, the thermal stability and pH tolerance of the two xylanase mutants are obviously improved. In the aspect of specific activity, when the beech xylan is used as a substrate, the specific activity of the two xylanase mutants is obviously improved compared with that of wild type xylanase. The invention provides a theoretical reference for the improvement of xylanase, and the obtained xylanase mutant with synchronously improved thermal stability and pH tolerance has a huge application prospect in the aspects of animal feed and biomass degradation sugar production.

The invention relates to the technical field of gene engineering, in particular to a tomato SlHXK1 gene and application of the tomato SlHXK1 gene to improvement of weak light tolerance of tomato seedlings. The nucleotide sequence of the tomato SlHXK1 gene is as shown in SEQ ID No. 1. Research finds that the overexpression of the SlHXK1 gene can improve the activity of sucrose anabolic enzymes in tomato stems and increase distribution of dry substances to leaves, so that the content of non-structural carbohydrates in tomato leaves under weak light is increased, the content of carotenoids and soluble proteins in the leaves is increased, and the photosynthetic ability of the leaves is enhanced; therefore, the adverse effect of weak light on dry matter accumulation of tomato seedlings is reduced, the accumulation rate of dry matter of leaves after illumination recovery is promoted, and the weak light tolerance of tomato plants is improved. Therefore, the invention provides a new gene and method for improving the weak light tolerance of the tomatoes, and has important significance for guaranteeing high yield, high quality and annual supply of the tomatoes in China.

The invention provides a biological fermentation preparation system process of 5-hydroxytryptophan, the system comprises a fermentation culture medium preparation and inoculation module, a fermentation tank, a sensor data acquisition module, a data processing unit and a single-chip microcomputer, and the process comprises strain optimization, fermentation, fermentation completion and product extraction. Wherein during fermentation, an efficient monitoring and control system is established, the fermentation environment is accurately controlled, changes of various key parameter data in the fermentation process are obtained, all the key parameter data are gathered through a data acquisition module and transmitted to a single-chip microcomputer, and the single-chip microcomputer automatically adjusts the working state of corresponding equipment according to fermentation parameters set by an algorithm. Through combination of genetic engineering strain optimization, an intelligent control system, membrane filtration and a reverse osmosis purification technology, the yield and purity of 5-HTP are remarkably improved, the production cost is effectively reduced, and the production period is shortened. Compared with the traditional process, the method has obvious advantages in multiple aspects, and has wide industrial application potential.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a TaMGD3 gene in improvement of plant starch quality and cultivation of a high-starch transgenic plant, including application of the TaMGD3 gene in promotion of wheat grain starch synthesis, application of the TaMGD3 gene in cultivation of the high-starch and / or high-amylopectin transgenic plant, and application of the TaMGD3 gene in improvement of plant starch quality and cultivation of the high-starch and / or high-amylopectin transgenic plant. The invention also discloses an application of the TaMGD3 gene in improvement of plant starch characteristics. A nucleotide sequence of the TaMGD3 gene is shown as SEQ ID NO. 1. The invention also provides a cultivation method of transgenic wheat. In wheat cultivation, wheat amylopectin quality improvement is realized through overexpression of the gene shown in SEQ ID NO.1. The TaMGD3 gene can improve the starch content of wheat grains, influence the particle size distribution of the wheat grain starch, improve the amylopectin proportion, improve the peak viscosity and the relative crystallinity of the starch, and facilitate the improvement of the cooking quality related to the wheat starch.

The invention discloses a fusion antibacterial peptide BMAP18-BSN37 and application thereof, and belongs to the technical field of gene engineering. The nucleotide sequence of the fusion antibacterial peptide BMAP18-BSN37 is as shown in SEQ ID NO. 1, and the amino acid sequence of the fusion antibacterial peptide BMAP18-BSN37 is as shown in SEQ ID NO. 2. The fusion antibacterial peptide BMAP18-BSN37 gene is inserted into a pNZ8148 expression vector to construct a recombinant expression vector pUBB, so that protein aggregation or degradation in cells can be avoided, and the survival rate of strains and the stability of protein expression are improved. The method comprises the following steps: transferring a plasmid pUBB into a lactic acid bacteria NZ9000 strain through electrotransformation, and screening a recombinant lactic acid bacteria strain capable of expressing a fusion protein BMAP18-BSN37 to obtain a recombinant strain NZ-BB; the recombinant lactic acid bacteria NZ-BB strain shows a remarkable prevention effect in an animal infection model, and can effectively reduce bacterial colonization and relieve pathological injury; the Salmonella strain has the potential of being used as an animal feed additive, can reduce the use of antibiotics and chemicals, slow down the development of bacterial drug resistance, improve animal immunity and slow down intestinal inflammation caused by salmonella, and provides support for effectively preventing the health of livestock and poultry.