331 results about "Genetically engineered" patented technology

The present invention relates to crop plants, particularly to plants of the Solanaceae family including tomato (Solarium lycopersicum) plants genetically engineered to have reduced expression and / or activity of SnRK2.3 protein and producing higher yield compared to a corresponding crop plant not engineered to have reduced expression and / or activity of the SnRK2.3 protein.

The invention provides a method for strengthening plant microorganism combined remediation of heavy metal contaminated soil, and relates to the technical field of soil remediation. The method for strengthening the plant and microorganism combined remediation of the heavy metal contaminated soil comprises the following steps: a) selecting and planting pioneer plants with tolerance to target heavy metal; b) applying a compound microbial agent containing at least two functional microorganisms, wherein the functional microorganisms comprise plant growth-promoting bacteria and microorganisms forming a symbiotic relationship with plants; c) loading the functional microorganisms on a biochar carrier, and applying the biochar carrier to soil; d) selectively adding an exogenous chelating agent according to the effective state concentration of the heavy metals in the soil; and e) selectively carrying out genetic engineering modification on the functional microorganisms or pioneer plants according to repair requirements. The technology is high in flexibility, optimization combination can be carried out according to different heavy metal types, combined pollution and soil conditions, and the advantages of high efficiency, environmental safety and long-term sustainability of the remediation process are achieved.

The application provides a preparation method and application of oat active peptides for promoting GLP-1 secretion, wherein the oat active peptides contain any one or more of peptide segments NDQRGEII, KQGDVIALPA, PFVQQQQ and PSEQYQPYPEQQEPFVQ. The preparation method of the oat active peptides comprises enzymolysis, chemical synthesis or genetic engineering. The oat active peptides can significantly promote the secretion of GLP-1 by intestinal endocrine cells, and have the advantages of safe and non-toxic side effects, tolerance to gastrointestinal digestive enzyme hydrolysis and easy absorption. The application has simple operation, low requirement for equipment, is suitable for industrial large-scale production, and can be used for the development of new drugs for controlling blood sugar or food for assisting in controlling blood sugar, drugs for controlling body weight or food for assisting in controlling body weight and the like.

The invention discloses application of GmERF5 in regulation and control of soybean grain size, and belongs to the technical field of gene engineering. The amino acid sequence of the encoding protein of the gene GmERF5 is as shown in SEQ ID NO.1, the CDS sequence of the gene is as shown in SEQ ID NO.2, and the full-length nucleotide sequence of the gene is as shown in SEQ ID NO.3. A series of experiments find and verify that the gene or the encoded protein thereof has a positive regulation effect on the size of soybean seeds, and by improving the expression level of the gene or the encoded protein thereof, the weight, the length, the width and the cross section area of the soybean seeds are increased, so that a new thought is provided for cultivating high-yield soybeans.

Described herein are genetically engineered enzymes having amylase enzyme activity, compositions comprising the enzymes, and methods of making and using the enzymes. The genetically engineered amylase enzymes are useful in many different applications such as laundry detergents, dish washing detergents, and cleaning products for homes, industry, vehicle care, baking, animal feed, pulp and paper processing, starch processing, brewing, and ethanol production.

The invention discloses application of an FYVE4 gene in improving nitrogen deficiency nutrition stress tolerance of plants. Belongs to the technical field of plant genetic engineering. The invention provides nucleotide and corresponding amino acid sequences and specific application thereof. The invention proves for the first time that the arabidopsis thaliana gene AtFYVE4 regulates the nitrogen deficiency stress response of the plant through an autophagy way. It is found that the arabidopsis thaliana nitrogen deficiency nutrition stress regulation related gene regulates the autophagy activity of arabidopsis thaliana through interaction with the autophagy protein AtATG1C, and then regulates the tolerance of plants to nitrogen deficiency nutrition stress. The application of the AtFYVE4 gene can provide support for drought resistance research of grain crops such as corn, rice, wheat and the like and other crops.

The application discloses a sequence of Medicago truncatula AP2 / ERF transcription factor ERM1 and application of the sequence in improving mycorrhizal colonization efficiency of leguminous plants and promoting mycorrhizal symbiosis, and relates to the technical field of plant genetic engineering. ERM1 The gene has a significant promoting effect on the symbiosis of alfalfa and arbuscular mycorrhizal fungi, and the application has important significance for clarifying the biological function of ERM1 in the symbiosis of leguminous plants and mycorrhizal fungi, thereby regulating the symbiosis between plants and mycorrhizal fungi, and provides a research foundation and scientific basis for improving the symbiosis efficiency of leguminous crops by using biological strengthening means.

This invention provides a dengue / Zika virus genetically engineered vaccine and its application. The dengue / Zika virus vaccine comprises an open reading frame encoding envelope protein domain III (EDIII) and the non-structural protein NS1, and displays EDIII monomers in the delivery vector shell. Immunization with this vaccine primarily induces a type-specific antibody response, reducing the production of cross-antibodies and thus effectively avoiding or eliminating the risk of antibody-dependent enhancement of infection (ADE). This vaccine can be used to prevent dengue virus and Zika virus infection.

The invention relates to the technical field of gene engineering, in particular to application of a knockout SlABCB13 gene in increasing the soluble sugar content of tomato fruits. The function of the SlABCB13 gene is deleted by mediating gene editing, so that the content of soluble sugar (cane sugar, fructose and glucose) and the content of soluble solids in tomato fruits are remarkably increased, and the SlABCB13 participates in sugar transport of the tomato fruits. Meanwhile, the mutant does not influence related yield indexes such as plant height, stem diameter, fruit size, fruit setting number and single-plant fruit yield, and a new thought is provided for the problem that yield and quality are difficult to consider in existing tomato breeding.

According to the HMO concentrated solution and the preparation method thereof, the mass concentration of HMO in the HMO concentrated solution is 60% or above, the HMO concentrated solution does not contain genetically engineered microorganisms, nucleic acid molecules derived from the genetically engineered microorganisms and protein and organic solvents, the purity of the HMO is at least 95%, and the content of the HMO in the HMO concentrated solution is more than 10%. The grain size of 90% of crystals in the HMO concentrated solution is not greater than 150 microns. The preparation method comprises the following steps: separating and purifying HMO fermentation liquor prepared by a microbial fermentation method, concentrating to 60 wt% or above, cooling to-10-10 DEG C within 0.5-2 hours, keeping stirring in the cooling process, cooling to a target temperature, and continuously keeping stirring for 1-4 hours. According to the invention, the stability of the high-concentration HMO concentrated solution in a refrigeration state can be realized.

The invention discloses wheat TaPAB1 protein and application of related biological materials of the wheat TaPAB1 protein in the aspect of improving the grain weight of wheat. A gene for coding the TaPAB1 protein is named as a TaPAB1 gene. According to the invention, the gene TaPAB1 is cloned from a wheat cultivation variety, Chinese spring, and is over-expressed in a wheat variety Kenong 199 (KN199) by utilizing a gene engineering technology, so that the thousand seed weight, the grain length, the plant height, the ear length and the yield of a TaPAB1 over-expressed plant are obviously increased compared with those of a wild type. The invention provides a research basis for increasing the wheat yield and breeding high-yield varieties.

The application belongs to the technical field of genetic engineering, and particularly relates to a soybean unconventional small peptide miPEP160b, a gene and application thereof. The application provides a soybean unconventional small peptide miPEP160b, and an amino acid sequence of the soybean unconventional small peptide miPEP160b is shown as SEQ ID NO. 2. The soybean unconventional small peptide miPEP160b can regulate symbiotic nodule formation of legume plants, can be used as a gene resource for biological breeding, and can be used for cultivating new varieties of legume plants with improved nodule formation ability and nitrogen fixation efficiency by using biological materials related to the soybean unconventional small peptide miPEP160b, thereby providing a new idea for soybean biological breeding and agricultural production application. In addition, the soybean unconventional small peptide miPEP160b can be used as a plant stimulant, can promote nodule formation ability and nitrogen fixation efficiency of soybeans by spraying around the roots of drip irrigation soybeans, and can also promote growth and development of soybean plants.

Disclosed herein is a genetically engineered yeast cell capable of producing xylitol, the genetically engineered yeast cell characterized by a genetic modification resulting in overexpression of a natural enzyme having xylitol-5-phosphate phosphatase (X5PP) activity and / or an exogenous polynucleotide sequence encoding an enzyme having xylitol-5-phosphate phosphatase (X5PP) activity. The genetically engineered yeast cell may additionally be engineered to overexpress a native RPE enzyme, to express an exogenous XPDH enzyme, to express an exogenous XKS enzyme, and / or to express an exogenous XDH enzyme.

The invention relates to a plant genetic engineering technology, in particular to a method for producing insect pheromone precursor substances in soybeans. Specifically, transgenic soybeans for expressing exogenous calyx flower plant thiolipase and insect desaturase are obtained through a genetic engineering technology, the transgenic soybeans can synthesize insect pheromone precursor substance cis-11-hexadecenoic acid, and therefore the biological efficiency and feasibility of plant factory pheromone production are proved. Furthermore, as the soybean is used as an oil crop with high acre yield, the soybean naturally contains high-content palmitic acid (C16: 0), the acre yield of the insect pheromone precursor substance cis-11-hexadecenoic acid is predicted to be far higher than that of other plants, and the plant is an ideal biological factory for mass production of the insect pheromone precursor substance cis-11-hexadecenoic acid.

Disclosed herein are genetically engineered Kluyveromyces marxianus cells capable of producing D-allulose with increased talitol formation. The engineered cell may comprise a genetic modification resulting in overexpression of a native talitol dehydrogenase enzyme; an exogenous polynucleotide sequence encoding an allulose-6-phosphate 3-epimerase enzyme at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:249-256, 258, and 259; and an exogenous polynucleotide sequence encoding an allulose-6-phosphate phosphatase enzyme at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:87, 89, 190, 123, 105, 107, 115, 83, 95, 113, 117, 119, 121, 127, 131, 137, 145, 169, 173, 179, and 183.

The invention discloses a gene related to sweet potato vine length and application of the gene in cultivation of short-vine sweet potatoes, and belongs to the technical field of gene engineering. The gene code is an amino acid sequence as shown in SEQ ID NO. 2. By improving the expression of the gene, the vine length of a sweet potato plant can be shortened, the stem internode of the sweet potato is shortened, and the leaves are denser. The gene is expected to regulate and control the vine length and / or internode length of sweet potatoes and improve germplasm resources of sweet potatoes, and has high economic and application values.

The invention discloses a phytase mutant with improved enzymatic activity and thermal stability as well as a preparation method and application thereof, and relates to the technical field of gene engineering and enzyme engineering. The amino acid sequence of the phytase mutant (named as Y111C / Q113L) is as shown in SEQ ID NO. 1. According to the invention, site-directed mutagenesis is carried out on amino acid residues which may influence the enzymatic activity and thermal stability of phytase YrAPPA in phytase YrAPPA, and mutants Y111C and Q113L with significantly improved enzymatic activity and thermal stability are constructed and screened. On the basis, the two-site mutant Y111C / Q113L with the phytase activity and the thermal stability further improved is further constructed and obtained. The enzyme activity and thermal stability of the phytase mutant provided by the invention are obviously improved, and the phytase mutant has a good application prospect in the field of animal feed additives.

The invention discloses a potato protein with good emulsibility, gelling property and foamability, and belongs to the fields of biology and genetic engineering. According to the invention, the designed potato protein realizes recombinant expression in pichia pastoris, and it is verified that the recombinant expressed protein can form a secondary structure and can form hydrogel at a concentration of 60 g / L. The invention also provides an application of the prepared potato protein as a food additive, shows emulsifying and gelling capabilities superior to those of commercial potato protein, and has huge value and potential in the fields of food and tissue engineering.

The invention provides application of FveABI4 in regulating and controlling the size of forest strawberry fruits, and belongs to the technical field of genetic engineering. Researches find that overexpression of FveABI4 can increase the size of fruits, promote the ripening process of the fruits, improve accumulation of soluble sugar in the fruits and reduce the content of citric acid in the fruits; and the reverse effect can be achieved by knocking down or knocking out the FveABI4. Therefore, the fruit size is regulated and controlled by intervening the expression of the FveABI4, and the ripening process and the sugar acidity of the fruits can be controlled at the same time, so that the problems that the inherent fruits of the forest strawberries are small and the separation of excellent characters of traditional breeding is serious can be ideally solved, and the application potential of the forest strawberries in the aspects of edible characters, processing characters, ornamental characters and the like is fully released.

This invention relates to the cassava MeABF gene and its applications, belonging to the field of plant genetic engineering and breeding technology. This invention discloses a cassava MeABF gene (… MeABF3 The application of cassava in regulating stress resistance was disclosed in detail. MeABF3 The gene can respond to drought and low temperature stress, positively regulating the drought resistance and cold tolerance of cassava. Utilizing the present invention... MeABF3 Genetically bred stress-resistant cassava materials can significantly improve the survival and growth performance of cassava under drought and low temperature conditions, expand the planting range of cassava, provide a molecular basis for high and stable cassava yield, and have important application prospects and economic value.

The invention discloses an efficient preparation and application method of antibacterial peptide special for livestock and poultry, and belongs to the technical field of bioengineering and livestock and poultry breeding. Aiming at the problems that the existing antibacterial peptide preparation process is complicated, the yield is low, the targeting property is poor, the antibacterial peptide is easily degraded by gastrointestinal tracts of livestock and poultry in application, and the balance of intestinal flora is damaged, the pichia pastoris modified by genetic engineering is adopted as an expression host, and the efficient secretory expression of the antibacterial peptide is realized by optimizing a two-stage fermentation process and a culture medium formula. Complex purification steps are not needed, the yield of the antibacterial peptide reaches 85% or above, the activity retention rate is increased by 40%, the intestinal pathogenic bacteria of livestock and poultry can be specifically inhibited, no damage is caused to probiotics, the antibacterial peptide can be prepared into feed additives, oral preparations and the like to be applied to livestock and poultry breeding, antibiotic dependence is reduced, the breeding cost is reduced, and remarkable economic and ecological benefits are achieved.

The present disclosure provides genetically engineered microorganisms for the simultaneous fermentation of pentose and hexose sugars, for example, glucose and xylose. The microorganisms can be modified to express AtSWEET polypeptides, LST1 polypeptides, mutants thereof, homologs thereof or combinations thereof. Also provided are methods of co-fermenting hexose and pentose sugars, methods of increasing the conversion of lignocellulosic biomass via microbial fermentation, and methods of generating biofuel.

The invention relates to the technical field of plant genetic engineering and agricultural planting, in particular to application and method of an SlGRXC9 gene in improvement of tomato cadmium stress tolerance, the number of the SlGRXC9 gene is Solyc07g053550, the core coding region sequence of the SlGRXC9 gene is SEQ ID NO.1, and the coded protein sequence is SEQ ID NO.2; through construction of SlGRXC9 overexpression transgenic tomatoes, heterologous expression of protein encoded by the gene in plant cells or use of the gene as a molecular breeding target, the system activity of antioxidant enzymes such as SOD, POD, CAT and GR in tomatoes can be enhanced, cadmium stress induced H2O2 and O2-accumulation and membrane lipid peroxidation damage are reduced, and absorption and accumulation of the tomatoes to cadmium ions are not affected; according to the method, the growth ability and physiological stability of the tomatoes under cadmium stress are remarkably improved, the risk of nutrient imbalance is avoided, the hereditary stability is high, and the method can be widely applied to safe production of the tomatoes in cadmium-contaminated soil and creation of stress-tolerant germplasm.

The application discloses application of an apple MdMYS1 gene in regulation of wax content of plant fruits and leaves and belongs to the technical field of plant genetic engineering. The application separates a MdMYS1 gene with high expression in an apple variety with high wax content, and the nucleotide sequence of the MdMYS1 gene is shown as SEQ ID NO. 1. Subcellular localization shows that a transcription factor expressed by the MdMYS1 gene is located on a cell nucleus. Experimental results show that overexpression of the MdMYS1 gene can significantly improve the wax content of apple fruits and leaves by promoting biosynthesis of the wax, and the MdMYS1 gene plays a key role in regulation of the wax content of apple fruits and leaves. The application provides an efficient and rapid way for apple breeding, provides gene materials for improvement of apple quality, and has wide application prospects in improvement of economic and ecological benefits of apple planting.

The invention belongs to the field of gene engineering, and discloses a method for rapidly evaluating the plant growth capacity of transgenic herbicide-resistant cotton in the seedling stage, and the method comprises the following steps: S1, sowing transgenic cotton seeds to be detected; s2, when cotton cotyledons are completely flattened, glyphosate with the working concentration of 0 time or 2 times or 4 times is sprayed; s3, 7 days after spraying, respectively taking the overground part and rhizosphere soil of the cotton, and detecting the dry weight of the overground part and the water content and microbiome of the rhizosphere soil; and S4, judging the plant growth capability of the transgenic herbicide-resistant cotton in the seedling stage. The method provided by the invention is a rapid, standardized and repeatable seedling stage plant growth ability evaluation method, is suitable for multi-material and large-scale determination, is easy to popularize and apply in the agricultural field, and has urgent and important practical significance for accelerating glyphosate-resistant cotton variety breeding and industrial application.

The invention discloses a recombinant bacterium for producing mevalonic acid by taking glucose and acetone as co-substrates as well as a construction method and application of the recombinant bacterium, and belongs to the technical field of genetic engineering. The problems that an existing mevalonic acid biosynthesis mode is low in carbon atom economy, limited in theoretical yield and the like are solved. According to the invention, acetone carboxylase, acetoacetyl coenzyme A synthetase, 3-hydroxy-3-methylglutaryl coenzyme A reductase and hydroxymethyl glutaryl-CoA synthetase are subjected to heterologous expression in escherichia coli with an acetyl coenzyme A acetyltransferase gene atoB and a histidine protein kinase gene atoS knocked out, and self carbonic anhydrase is over-expressed; and a new way for synthesizing MVA by using glucose and acetone as a co-substrate is constructed. According to the method, one molecule of CO2 can be fixed in the biosynthesis of mevalonic acid, the yield of carbon atoms in the synthesis process of mevalonic acid is increased, the problem of low yield of carbon atoms in the synthesis process of mevalonic acid by taking glucose as a single substrate is solved, and a new thought is provided for constructing a carbon neutralization type biological manufacturing technology.