227 results about "Chemical coupling" patented technology

Normal cells, such as fibroblasts or other tissue or organ cell types, are genetically engineered to express biologically active, therapeutic agents, such as proteins that are normally produced in small amounts, for example, MIS, or other members of the TGF-beta family Herceptin(TM), interferons, andanti-angiogenic factors. These cells are seeded into a matrix for implantation into the patient to be treated. Cells may also be engineered to include a lethal gene, so that implanted cells can be destroyed once treatment is completed. Cells can be implanted in a variety of different matrices. In a preferred embodiment, these matrices are implantable and biodegradable over a period of time equal to or less than the expected period of treatment, when cells engraft to form a functional tissue producing the desired biologically active agent. Implantation may be ectopic or in some cases orthotopic. Representative cell types include tissue specific cells, progenitor cells, and stem cells. Matrices can be formed of synthetic or natural materials, by chemical coupling at the time of implantation, using standard techniques for formation of fibrous matrices from polymeric fibers, and using micromachining or microfabrication techniques. These devices and strategies are used as delivery systems via standard or minimally invasive implantation techniques for any number of parenterally deliverable recombinant proteins, particularly those that are difficult to produce in large amounts and / or active forms using conventional methods of purification, for the treatment of a variety of conditions that produce abnormal growth, including treatment of malignant and benign neoplasias, vascular malformations (hemangiomas), inflammatory conditions, keloid formation, abdominal or plural adhesions, endometriosis, congenital or endocrine abnormalities, and other conditions that can produce abnormal growth such as infection. Efficacy of treatment with the therapeutic biologicals is detected by determining specific criteria, for example, cessation of cell proliferation, regression of abnormal tissue, or cell death, or expression of genes or proteins reflecting the above.

Normal cells, such as fibroblasts or other tissue or organ cell types, are genetically engineered to express biologically active, therapeutic agents, such as proteins that are normally produced in small amounts, for example, MIS, or other members of the TGF-beta family Herceptin(TM), interferons, andanti-angiogenic factors. These cells are seeded into a matrix for implantation into the patient to be treated. Cells may also be engineered to include a lethal gene, so that implanted cells can be destroyed once treatment is completed. Cells can be implanted in a variety of different matrices. In a preferred embodiment, these matrices are implantable and biodegradable over a period of time equal to or less than the expected period of treatment, when cells engraft to form a functional tissue producing the desired biologically active agent. Implantation may be ectopic or in some cases orthotopic. Representative cell types include tissue specific cells, progenitor cells, and stem cells. Matrices can be formed of synthetic or natural materials, by chemical coupling at the time of implantation, using standard techniques for formation of fibrous matrices from polymeric fibers, and using micromachining or microfabrication techniques. These devices and strategies are used as delivery systems via standard or minimally invasive implantation techniques for any number of parenterally deliverable recombinant proteins, particularly those that are difficult to produce in large amounts and / or active forms using conventional methods of purification, for the treatment of a variety of conditions that produce abnormal growth, including treatment of malignant and benign neoplasias, vascular malformations (hemangiomas), inflammatory conditions, keloid formation, abdominal or plural adhesions, endometriosis, congenital or endocrine abnormalities, and other conditions that can produce abnormal growth such as infection. Efficacy of treatment with the therapeutic biologicals is detected by determining specific criteria, for example, cessation of cell proliferation, regression of abnormal tissue, or cell death, or expression of genes or proteins reflecting the above.

The invention relates to a biodegradable stent composite material and a preparation method thereof. The degradable material has a multi-gradient composite structure, and comprises a matrix made of a medical metal or an alloy thereof, a chemical coupling layer attached to the surface of the matrix, a high polymer transition layer attached to the surface of the chemical coupling layer and a degradable high polymer functional layer fixed on the surface of the high polymer transition layer. The stent composite material has high mechanical property, degradation property and biocompatibility, also can absorb and carry needed therapeutic medicaments, and meets the requirement on clinical treatment. The degradable composite material can be used as stent materials of esophagi, biliary ducts, intestinal tracts, urethrae, tracheae and other non-vessel lumens and blood vessels, and also can be used for manufacturing artificial bones, bone nails, bone connectors, bone sutures, anchors for suture, intervertebral discs, hemostatic clamps, hemostatic forceps, hemostatic plates, hemostatic screws, tissue adhesives, sealants and other medical devices and products.

The invention discloses a temperature-humidity-stress-chemical coupling test platform for porous dielectric material. The test platform comprises a closed container, a load-bearing frame, a mechanical test system, an environment simulating system and a data acquiring system; the load-bearing structure is fixedly arranged at upper and lower two ends of the closed container; the mechanical test system comprises a loading device and a power device; the loading device is arranged in the enclosed container; the environment simulating system comprises a temperature simulating device and a humidity simulating device; the environment simulating system further comprises a chemical simulating device; the chemical simulating device comprises a chemical gas generator and a gas pipeline; the outlet of the gas pipeline is connected in the closed container; and a gas analyzer used for monitoring gas component and concentration is further arranged in the closed container. By adopting the test platform provided by the invention, the temperature and humidity changes in atmosphere environment and the influence of gas containing chemical substance to the long-term mechanical property of material can be simulated; and the test platform is feasible for mechanical multi-field coupling research on materials.

The invention discloses a full-separating method of high-purity rare earth oxide from low-yttrium middle-heavy rare earth rare earth ore, which is characterized by the following: adopting enriched yttrium rare earth ore as raw material; producing high-purity Y2O3, La2O3, Ce2O3, Pr5O11, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Tb4O7, Dy2O3, Er2O3 and Y2O3 through ultrasonic extracting-electrochemical, quantivalence-changing and chemical coupling technique; obtaining the product.

The invention relates to an immobilized enzyme biocatalyst and the manufacturing method and application. The immobilized enzyme biocatalyst regards tubular hollow silica dioxide dielectric hole material as a carrier external surface, inner surface and micropore in the wall of which are fixed biological enzyme molecule. The method includes physical adsorption cast investment or chemical coupling / crosslinking method; the biocatalyst is provided with good enzyme dispersibility and high carrying quantity, high recovery ratio of enzymatic activity, low enzyme flow rate. The invention can fix penicillin acylating enzyme, glucose oxidase, peroxidase, cytase and so on, which also can be used for removing sugar in protein, removing sugar in total egg, removing oxygen in food , microbiological sensing device, antibiosis and disinfection reaction, wherein immobilized penicillin acylating enzyme biocatalyst can be used to hydrolyze penicillin potassium and manufacture 6-APA.

The invention relates to a CT real-time scanning triaxial stress, seepage and chemical coupling rheology test system. The system includes a confining pressure chamber (A), an axial pressure chamber (B) and a test chamber (C), the confining pressure chamber (A) comprises a confining pressure chamber cylinder (1) and a confining pressure chamber pedestal (2), and the confining pressure cylinder (1) and the confining pressure cylinder pedestal (2) are fixed and sealed through a bottom screw (3); and the axial pressure chamber (B) includes an axial piston (4), an axial pressure cylinder and an axial pressure cylinder top cover (6), and the system also includes a seepage part, a triaxial stress closed-loop part and a chemical coupling part. The dimension of the triaxial pressure chamber of the test system is small, so the problem of large dimension of the triaxial pressure chamber is solved; and three closed-loop servo metering pumps are adopted to respectively precisely control the confining pressure, the axial pressure and the osmotic pressure, and can provide the pressure sources for MHC coupling rheology test, so a problem that present hydraulic stations are not suitable for long time work is solved, and test data is reasonable and accurate.

The invention discloses a method for testing concrete performance under pressure stress-chemical coupling effect and a device thereof. The method applies a pressure stress to a test piece through a prestressing mode, and provides specific chemical corrosion action through a matched sealing device so as to apply a pull force to concrete and apply common chemical action at the same time. The internal stress of the concrete is monitored through a pressure sensor, the pressure stress is kept constant through secondary tension prestressing force, and the sealing device is arranged on the middle part of the concrete test piece to provide the chemical action, so the corrosion action of the chemical medium on a loading device is avoided; and a sealed box can provide one or more chemical erosion environments of carbonization, sulfate erosion, alkali-aggregate reaction, chloride ion erosion and corrosion. The device has a string structure, and can conveniently apply the pressure stress-chemical coupling effects of different conditions to a plurality of concrete test pieces.

A blood purification sorbent for antibody removal belongs to the technical field of biomedicine, which consists of the two parts of solid-phase carrier material and a petunidin fixed on the carrier by chemical coupling. The molecular structure of the petunidin is shown as above, wherein, one atom among A, B and C is N, the others are C; n is 0-2. The blood purification sorbent can adsorb the antibody component in plasma and autoantibodies such as rheumatoid factor, antinuclear antibody, etc. in heavy load, has limited nonspecific adsorption to other plasma components such as seralbumin, etc., and also has low preparation cost and stable physicochemical property. The material can be used as adsorption filler of a blood purification device for removing the autoantibody and immune complex in the plasma.

The invention discloses a fluorine-containing functional diamine monomer with a large conjugated structure as well as a synthesis method and an application thereof. The synthesis method comprises the following steps of: firstly allowing di-halogenated fluorine to react with halogenated nitrobenzene, linking halogen atoms with other chemical groups to form a large conjugated system, and finally reducing binitro into diamine; or firstly carrying out a chemical coupling reaction between two active hydrogen atoms in fluorine to form the large conjugated structure, carrying out a Suzuki reaction on the large conjugated structure and two halogen atoms to obtain the diamine, and finally obtaining the novel fluorine-containing functional diamine monomer. By means of the specific structure of the diamine monomer, the polymer obtained by the diamine monomer has the advantage of good solubility, and the diamine monomer and the polymer can also achieve certain photoelectric properties; and the synthesis method has the advantages of simple process and easy purification procedure, thus being applicable to industrial production. The fluorine-containing functional diamine monomer has obvious characteristics of luminescence aggregation and induction, thus the diamine monomer can be used for synthesizing high-performance functional polymers such as polyamide, polyimide, polyamideimide, polyesterimide and the like.

The present invention provides a device and method for synthesizing nucleic acid-templated combinatorial chemical libraries. The device includes a splitting filter having an array of immobilized capture nucleic acids and a chemical coupling filter having an array of non-specific binding features, wherein the plates are positioned to provide for alignment between the capture sites of the first filter and the non-specific binding features of the second filter plate. The molecules bound to the splitting filter can be transferred to the chemical coupling filter and then reacted with site-specific reagents to chemically modify the bound molecules.

The invention provides a rock-soil body shearing rheometer considering seepage-stress-chemical coupling. The rock-soil body shearing rheometer considering seepage-stress-chemical coupling comprises a test bench, a shearing box assembly, a loading assembly and a displacement-stress monitoring assembly; a large-size test sample can be placed in a shearing box unit, chemical liquid with a certain osmotic pressure is provided through a water pump, the test sample is dried through a hot air circulating unit, and seepage of the chemical liquid with different osmotic pressure and dry-wet circulation of the test sample are realized; normal pressure is loaded by weights, tangential pressure is loaded by a rigid spring, a vibration exciter acts on a spring so as to generate disturbance on tangential stress, and the deformation amount of the rigid spring is maintained by controlling a linear displacement mechanism to guarantee stability of the tangential stress; the displacement-stress monitoring assembly cooperates with a corresponding data acquisition system to monitor the displacement of a lower shearing box and the tensile force of the test sample. The rock-soil body shearing rheometer considering seepage-stress-chemical coupling can research the problems about rock-soil body disturbed by power, underwater, chemical corrosion, dry-wet circulation and long-term stability of single or combined action of stress.

The invention belongs to the technical field of criminal science, and particularly relates to an aldehyde group modification method of surface functionalization nanometer upconversion materials, wherein the aldehyde group modification method is used for display of latent fingerprints. Aldehyde group reacts with amino acid residue in the latent fingerprints, is deposited on the fingerprints through chemical coupling, and can emit visible colored fluorescence under radiating of 980 nanometer infrared light, and therefore the latent fingerprints on the surface of an object can be cleared displayed. The surface functionalization nanometer upconversion materials have the advantages that the using method is simple and easy to carry out, functionalization particles can be stored for a long time, background interference is little, and the like.

The invention discloses a temperature-regulated phase material microcapsule carrier immobilized enzyme and a preparation method for the same, and relates to the technical field of biology. The preparation method comprises the following steps: preparing a microcapsule carrier obtained by cladding an organic phase change material with a crystalline titanium dioxide / magnetic particle composite material, performing carboxylation modification on the microcapsule carrier, and implementing enzyme immobilization by adopting a chemical coupling method, so as to finally obtain the temperature-regulated phase material microcapsule carrier immobilized enzyme. According to the temperature-regulated phase material microcapsule carrier immobilized enzyme and the preparation method for the same, heat can be stored and released by the microcapsule carrier of the immobilized enzyme through a phase change material capsule core, so that micro-environmental temperature around the carrier can be regulated, the temperature application range of enzymatic reaction can be widened, and the heat stability, the storage stability and the cycling stability of the immobilized enzyme can be enhanced. In addition, the temperature-regulated phase change material microcapsule carrier can be used for immobilizing different types of enzymes, and is wide in application range.

The invention discloses an antibody coating process and a myohemoglobin determination reagent kit made with the same. The problem that in the prior art, bridging of various chemical substances is generally adopted to improve the antibody and microsphere coupling efficiency, and consequently an antibody microsphere preparation process is more complex and higher in cost is solved. The process includes the steps that firstly, microspheres and an activator are dissolved in a buffer solution with the pH of 6.0 respectively; secondly, carboxyl on the surfaces of the microspheres is activated with an activator to obtain activated microspheres; thirdly, antibodies are added into the activated microspheres for coupling; fourthly, a buffer solution with the pH of 8.0 is added into the activated microspheres coupled with the antibodies to continue a reaction; fifthly, after the reaction is completed, a sealing agent is added to complete sealing. The process has the advantages that the chemical coupling efficiency is greatly improved, the operation steps are simplified, and the production cost is reduced.

The invention relates to a blood purification adsorbent which is used for eliminating antibody and the preparation method in the field of biomedical materials. The blood purification adsorbent comprises two parts of vector matrix and ligand, wherein, the ligand is fixed at the vector matrix by chemical coupling. The invention is characterized by taking benzoic acid molecular as the immobilized ligand of the blood purification adsorbent. The preparation method of the blood purification adsorbent takes cross-linking polysaccharide such as agarose, etc. as the vector matrix and prepares the adsorbent material by four steps of activation, arm hook, coupling matrix and post-processing. The invention has the advantages that: the invention can adsorb the antibody components in the plasma with high load; the invention has the adsorption limitation on nonspecific other plasma components such as serum albumin, etc.; the preparation cost is low; and the physicochemical properties are stable. The material can be used for eliminating the self antibody and the immune complex in the plasma as the adsorption filler of a blood purification device.

The invention discloses a rock temperature-permeation-stress-chemical coupling multifunctional test system and operation method thereof. The multifunctional test system comprises a vertical loading device (1), a chemical solution isolating device, a pressure control device, a pressure chamber (4), a volume change determinator (5), a temperature control device (6) and a computer (7); multiple geo-technical test operations are completed by controlling the vertical loading device (1), the chemical solution isolating device, the pressure control device, the pressure chamber (4), the volume change determinator (5) and the temperature control device (6) through the computer (7). The multifunctional test system disclosed by the invention is reasonable in structure design, simple in operation, wide in application range, high in automation degree, capable of realizing overall process automatic control of multiple geo-technical tests, high in stability, and long in service life.

The invention discloses an immobilized laccase microballoon carrier and its preparation method and use. The preparation method comprises carrying out phosphorylation modification on polyvinyl alcohol microballoons to obtain the immobilized laccase microballoon carrier. The immobilized laccase microballoon carrier can undergo chemical coupling and chelating reactions with laccase. The immobilized laccase microballoon carrier can be used for industrial wastewater treatment, can be used in the field of environment monitoring, and has the characteristics of large specific surface area, small particle size, good biocompatibility, stable physical and chemical properties and low cost.

The invention discloses a preparation method of a natural active substance constructed polymer composite medicine and application thereof in inhibiting angiogenesis. The polymer composite medicine is formed by introducing natural active small molecule substances on carboxyl of heparin or derivatives thereof by a chemical coupling method. According to the polymer composite medicine, the dissolubility of the natural active small molecule substances can be improved, the toxic and side effects are reduced, the problem of bleeding after the heparin or derivatives thereof are massively used can be improved, and the effect of inhibiting angiogenesis when the heparin or derivatives thereof are used independently can be reinforced. The polymer composite medicine is simple in preparation process, easy to operate, can reduce cost and improve efficiency, and can easily realize industrialization.

Disclosed is a method for refolding a protein or peptide that does not contain essential disulfides and that contains at least one free cysteine residue. Also disclosed are polymer IFN-γ conjugates that have been created by the chemical coupling of polymers such as polyethylene glycol moieties to IFN-γ, particularly via a free cysteine in the protein. Also disclosed are analogs of bioactive peptides that may be used to create longer acting versions of the peptides, including analogs of glucagon, glucagon-like peptide-1 (GLP-1), GLP-2, Gastric inhibitory peptide (GIP), PYY, exendin, ghrelin, gastrin, amylin, and oxyntomodulin.

The invention relates to a nanometer photosensitizer for photodynamic antisepsis, a preparation method and an application thereof. According to the invention, a photosensitizer grafted chitosan or a derivative thereof is acquired through the chemical coupling reaction of a photosensitizer and chitosan or chitosan derivative; such amphiphilic polymer can be self-assembled to form the nanometer photosensitizer; the hydrophobicity of a prototype photosensitizer is improved by the nanometer photosensitizer and the stability and biocompatibility thereof can be promoted; besides, the nanometer photosensitizer with positive charges is formed in a physiological solution by the photosensitizer grafted chitosan or the derivative thereof, pathogenic microorganisms can be better taken under the effectof electrostatic interaction, oxygen molecules in surroundings are converted into singlet oxygen under the effect of visible light / near-infrared light, and the singlet oxygen can be used for photodynamic antisepsis.

The invention discloses a cement reinforcing agent, a preparation method, and application thereof. The cement reinforcing agent is synthesized by using an amino silane coupling agent for modifying a wet-process-produced white carbon black product through a chemical coupling reaction. Relative to 100 parts by weight of wet-process white carbon black, the chemical modification process needs 500-1300 parts by weight of deionized water, 1-15 parts of a silane coupling modifier, 1-2 parts of an anionic surfactant, and 10-30 parts of an organic solvent. A national standard GB / T17671-1999 method is employed, the addition amount of the product is only 1.0-2.0 parts relative to the cement mass, and compared with 7-day strength of black cement, the strength is improved by about 30 times.

Disclosed is a method for refolding a protein or peptide that does not contain essential disulfides and that contains at least one free cysteine residue. Also disclosed are polymer IFN-γ conjugates that have been created by the chemical coupling of polymers such as polyethylene glycol moieties to IFN-γ, particularly via a free cysteine in the protein. Also disclosed are analogs of bioactive peptides that may be used to create longer acting versions of the peptides, including analogs of glucagon, glucagon-like peptide-1 (GLP-1), GLP-2, Gastric inhibitory peptide (GIP), PYY, exendin, ghrelin, gastrin, amylin, and oxyntomodulin.

The invention discloses a manufacturing method of a drug vessel support with antibody immobilized on the surface of the support. The manufacturing method comprises the following steps: 1) preparing polyester high polymer with carboxyl functional groups by using a synthesis or hydrolysis mode; 2) coating drug loading polyester high polymer on the surface of the support; and 3) immobilizing the antibody with the function of capturing endothelial progenitor cells on the surface of the support body by using a chemical coupling mode. In respect of the drug vessel support with antibody immobilized on the surface of the support, polyester high polymer with carboxyl functional groups is taken as the support drug loading coating to obtain the drug support the surface of which is subject to carboxylation, so that the surface of the support has both biocompatible compatibility and biological activity, and the carboxyl functionalized surface of the support provides abundant binding sites for loading effective dosage of antibody in the next step. Meanwhile, the support has a drug carrying function and an endothelial cell capturing function, thereby playing excellent roles in treating restenosis and promoting endothelial cell repairing.

The invention discloses a blue quantum dot composite particle and a method for preparing the same, a photoelectric element and a photoelectric device. The blue quantum dot composite particle comprises a core and a shell that covers the core, wherein the core is a blue quantum dot, the shell is formed of a polymer, and the surface of the shell is chemically modified with gold nano-granules. The method for preparing the blue quantum dot composite particle comprises: coating a polymer on the surface of a blue quantum dot via seed emulsion polymerization; modifying the polymer via hydrolysis so as to make the surface thereof have a carboxyl functional group; and combining a gold nano-granule having an amino functional group on its surface with a blue quantum dot having a carboxyl functional group on its surface via chemical coupling, and obtaining a blue quantum dot composite particle. The fluorescence efficiency of the blue quantum dot composite particle according to the invention is very high.

The invention discloses an experimental method for measuring the high-temperature mechanical-chemical coupling of a material by utilizing a nanoindentor, and belongs to the technical field of engineering materials, structural deformation and experiments in mechanics. The experimental method is technically characterized by comprising the following steps: performing an indentation experiment on a polished surface of a test piece after the temperature reaches a target temperature by utilizing a high-temperature environment provided by a nanoindentor, so as to obtain an indentation on the surface of the test piece, and maintaining the contact between a pressing head and the indentation, so that the material is oxidized in a certain time under the stress conditions; and analyzing and measuring the tensile stress of a pressing head action area on the surface of the material and the oxide layer thickness of a compressive stress area by virtue of a transmission electron microscope and an energy dispersion X-ray spectrometer after the experiment is ended, and calculating the oxidation rate of the material in different stress states. According to the experimental method disclosed by the invention, the oxidation rate of the material in the different stress states is measured under the micron and nano scales according to the specific experimental analysis result of the nanoindentor, and the mechanical-chemical coupling process and performance of the material are evaluated.

The invention relates to a preparation method for a molecularly imprinted polymer microballoon with a hydrophilic high-molecular brush. The molecularly imprinted polymer microballoon with the hydrophilic high-molecular brush is prepared by performing chemical coupling reaction on a molecularly imprinted polymer microballoon with the surface containing a C=C bond and a hydrophilic macro-molecule chain transferring agent in the presence of a common radial initiator. The molecularly imprinted polymer microballoon has the advantages of simple synthetic method, wide application scope, definite product structure and the like. The obtained molecularly imprinted polymer microballoon with the surface containing the hydrophilic high-molecular brush has wide application prospect in a plurality of fields such as medical clinic immunoassay, food safety, environment monitoring, biological technology and the like.

An optical biomodule for detecting a disease specific biomarker, utilizing an enhanced fluorescence emission (due to integration of one or more three-dimensional (3-D) protruded structures) in a fluidic container, upon chemical binding of a disease specific biomarker binder with its corresponding disease specific biomarker is disclosed. The three-dimensional (3-D) protruded structure can be coupled with a photonic crystal / metamaterial / metamaterial of Epsilon-Near-Zero (ENZ) of a suitable wavelength range. Furthermore, an enhanced fluorescence emission can be modulated by a synthetic Cas13 protein chemically coupled with a CRISPR RNA (crRNA) and a quenched fluorescent reporter. Alternatively, a suitable synthetic substitute of a synthetic Cas13 protein chemically coupled with a CRISPR RNA (crRNA) containing a targeting sequence can also be utilized.

The invention discloses a stress-chemical coupling visualization test system of interbedded salt rock, which includes a specimen holder, an axial chemical solution adding system, a confining pressure loading system, and a nuclear magnetic resonance imaging system; the holder includes a tubular device body, upper plug, lower plug, upper plug, lower plug, tubular sealing sleeve, heating layer, etc.; the test system of the present invention can conveniently control and adjust the pressure, temperature, The confining pressure and the temperature environment, and with the help of the nuclear magnetic resonance imaging system, can visually analyze the seepage path and seepage amount of oil in the bedding zone of the interbedded salt rock under a certain confining pressure condition; it can simulate when the surrounding rock is under stress After cracking and damage under action, liquid solution infiltrates along the cracks to produce different degrees of corrosion on salt rocks or interlayers, weakens the surrounding rocks, and promotes new cracks to produce this coupling superposition effect, revealing the stress-chemistry of bedding in the salt cavern oil storage process Coupling effects and permeability.