156 results about "Chemical coupling" patented technology

Provided are a thermal-hydrological-mechanical-chemical coupling exploitation experimental device for a deep oil and gas reservoir, and a simulation system. The device includes a loading bin, a sample holding bin, a true triaxial pressure loading mechanism arranged on the loading bin, a temperature control system arranged on the true triaxial pressure loading mechanism, and a monitoring system having a detection element arranged in the rock sample and the sample holding bin. A sample entry port is formed in one side surface of the loading bin. The sample holding bin has one end slidably arranged in the sample entry port, can be sealed and connected with the sample entry port, and is configured for placing and fixing a rock sample. Multiple through holes are uniformly formed on an outer end cover of the sample holding bin and are configured for detecting and adding water, gas, and oil.

The invention discloses a stem cell exosome based on targeted fusion polypeptide modification, a preparation method and application of the stem cell exosome in treating male erectile dysfunction. According to the exosome, a specific targeting fusion polypeptide (sequence: EPLQLKM-GGGS-DPPV) is anchored on the surface through a chemical coupling technology, so that the targeting property of the exosome on cavernosum smooth muscle cells is enhanced; meanwhile, an anti-TrkA monoclonal antibody (TrkA-2E7-HL) is loaded through an electroporation method, and a fibrosis pathway mediated by a nerve growth factor (NGF) is inhibited. The exosome induces secretion of the umbilical cord mesenchymal stem cells by adopting an anti-oxidation culture medium, so that the anti-oxidation activity of the exosome is remarkably improved. Experiments show that in a diabetes ED rat model, the erectile function (ICP / MAP ratio) of the modified exosome is improved to 0.79 + / -0.05, the fibrosis area is reduced to 9.2 + / -1.6%, and the NO secretion level is improved by 3.5 times compared with that of a common exosome. Through triple mechanisms of polypeptide targeting, antibody synergy and oxidation resistance, effective reversion of the ED pathological process is realized, and the polypeptide has remarkable novelty and clinical transformation potential.

The invention relates to the technical field of material surface engineering, and discloses a plastic surface treatment process to solve the problems that in the prior art, a plastic surface coating is poor in adhesive force and single in function, and a traditional treatment method is not environmentally friendly. The process sequentially comprises the following steps: pre-treating a base material, and obtaining a clean and stress-free base material through ultrasonic cleaning and thermodynamic stress elimination; performing low-temperature plasma surface activation, performing physical bombardment and chemical modification on the plastic surface by utilizing plasma of specific process gas in a vacuum environment, introducing a large number of active functional groups, and remarkably improving the surface energy; and preparing and applying the functional nano coating, namely forming firm covalent bonds by using the coupling agent in a coating solution containing functional nano particles and a coupling agent and the anchor points, so that the chemical coupling of the functional coating and the plastic substrate is realized.

The invention relates to the technical field of wind power generation materials, in particular to a rubber insulating material and a sheath material for wind power generation. The rubber insulating material for wind power generation comprises an insulating inner layer, an interface transition layer and a sheath outer layer, in the rubber insulating material for wind power generation and the sheath material, through double mechanisms of chemical coupling of a plant-based diluent and nano-cellulose and physical adsorption of expanded graphite, the insulating inner layer has excellent heat resistance and mechanical strength on the basis of keeping the heat resistance and mechanical strength of the material; the processing viscosity of the epoxy resin system is reduced; the outer layer of the sheath improves the mechanical property and salt mist erosion resistance of the material through the chemical anchoring of plant fibers and resin and the barrier effect of the nanosheet layer; meanwhile, the natural latex-cellulose nanocrystalline composite network of the interface transition layer strengthens the interface compatibility of the insulating layer and the sheath layer, and avoids the interlayer separation risk, thereby improving the reliability and service life of the material in an extreme environment of offshore wind power equipment.

The invention relates to preparation of 10-hydroxycamptothecine modified polypeptide and a research on cytotoxicity of the 10-hydroxycamptothecine modified polypeptide. The method comprises the following steps: synthesizing SA-7 polypeptide by adopting an F-moc solid-phase synthesis method, and combining the SA-7 polypeptide with 10-hydroxycamptothecine through chemical coupling reaction to obtain the SA-7-HCPT compound. C18 reversed-phase high performance liquid chromatography (RP-HPLC) is used for purification, and the molecular weight is identified to be 1898Da by using mass spectrum. A further cytotoxicity test shows that the SA-7-HCPT shows relatively strong anticancer activity to HepG2, and the toxicity of the SA-7-HCPT to HepG2 is remarkably superior to that of 10-HCPT to HepG2. Meanwhile, the toxicity of SA-7-HCPT to normal liver cells LO2 is relatively low, and the SA-7-HCPT shows good selectivity. Therefore, the SA-7-HCPT has a relatively good clinical application prospect.

The invention provides an electro-chemical coupling collaborative explosion near-field shock wave pressure measuring device and method. The device comprises a fiber grating sensing system; comprising an FBG optical fiber sensor, the FBG optical fiber sensor extends into an explosion cavity, the FBG optical fiber sensor is fixed on a flange of the explosion cavity, the FBG optical fiber sensor is connected with a circulator through an optical fiber patch cord, the circulator is connected with a laser light source, and the circulator is further connected with a coupler; the coupler is connected with the signal demodulator, and optical signals of the FBG optical fiber sensor are introduced into the signal demodulator; the signal demodulator is further connected with the photoelectric detector, the coupler is further connected with the photoelectric detector, and the photoelectric detector is connected with the oscilloscope to take electricity. According to the invention, strong electromagnetic pulse interference accompanied by explosion is overcome, and the impact wave pressure generated by metal wire electric explosion and micro-explosive chemical explosion can be more accurately measured. The technical problems of strong interference signals, overlarge sensor size and the like in the measurement of the explosion impact pressure of the tiny explosive quantity in the cavity are solved.

The invention discloses a monatomic electrocatalyst of a metal polyphenol network as well as a preparation method and application of the monatomic electrocatalyst. The preparation method comprises the following steps: adding an alkaline solution of Pluronic F127; polyphenol and formaldehyde are added for a cross-linking reaction; dropwise adding a metal salt solution, stirring and mixing, carrying out a hydrothermal reaction, carrying out centrifugal separation to obtain a precipitate, and washing and drying to obtain the monatomic catalyst. According to the method, polyphenol containing a large number of hydroxyl groups is taken as a ligand, coordination with metal ions is realized through formaldehyde-assisted crosslinking, a reaction path is precisely regulated and controlled through hydrogen bond engineering, a CO2 reduction reaction path is changed from a traditional * CO path to a low-energy barrier formyl path, C-C chemical coupling and hydrogen evolution / deuterium evolution side reaction are synchronously inhibited, and the reaction efficiency is improved. The selectivity of methane or deuterated methane is obviously improved, the operation stability for ultra-long time is realized, and an effective solution is provided for synthesis of methane and deuterated methane with high additional value.

The invention relates to the technical field of ceramic glaze production, in particular to interlayer chemical coupling long-acting noctilucent ceramic glaze as well as a preparation method and application thereof. The glaze is of a multi-layer composite structure and sequentially comprises a bottom-layer combined glaze layer, a core noctilucent functional glaze layer and a photochromic protective cover glaze layer from inside to outside. The core layer adopts a glaze system containing niobium pentoxide to realize chemical coupling with the bottom layer, and adopts a core-shell structure long afterglow luminescent material to realize white light adjustability; and the protective cover glaze layer adopts a WO3 / TiO2 / CeO2 composite system. The glaze is prepared through a two-step method of high-temperature primary firing and low-temperature secondary firing, and the obtained glaze is high in interlayer bonding strength, high in weather resistance, long in luminous life, capable of adapting to ambient light and suitable for safety sign products in extreme environments.

The invention relates to an RGD (arginine-glycine-aspartic acid) peptide modified mercaptododeborane derivative and a synthesis method, the derivative utilizes RGD peptide to perform chemical coupling modification on mercaptododeborane anion salt (BSH), and the BSH and the RGD peptide are connected through a hydrophilic intermediate connector with a double bond at one end and carboxyl at the other end. A hydrophilic chain is selectively introduced to serve as an intermediate connector, a series of compounds are synthesized through a convenient and efficient sulfydryl-alkene click reaction and an amino-carboxyl amidation condensation reaction and are purified, various cationic BSH salts and RGD peptide are connected, and targeted modification of the RGD peptide on an existing boron carrying agent BSH is achieved. The derivative obtained by the invention is not reported before, has the potential to improve the tumor targeting ability of the existing boron carrying agent, and is expected to be used as a drug compound for BNCT treatment.

The invention discloses a method for detecting silicon dioxide in kaolin based on a nanoprobe. The method comprises the following steps: grinding a kaolin sample, mixing with deionized water, performing ultrasonic dispersion, and centrifuging to obtain a kaolin sample solution; the preparation method comprises the following steps: preparing titanium dioxide nano-particles with amino groups modified on the surfaces by adopting a hydrothermal method, and modifying a silane coupling agent on the surfaces of the nano-particles by adopting a chemical coupling method to form a functionalized nano-probe; adding the functionalized nanoprobe into a kaolin sample solution; the nanoprobe is combined with silicon dioxide in a sample to form a nanoprobe-silicon dioxide compound; collecting a nano probe-silicon dioxide compound; reacting the nanoprobe-silicon dioxide compound with a fluorescent marker, and combining the fluorescent marker with silicon dioxide in the compound; and detecting the fluorescence intensity, and calculating the content of silicon dioxide in the sample according to the standard curve. The method for detecting silicon dioxide in kaolin is high in sensitivity, high in selectivity, simple and convenient to operate and wide in application.

The invention provides a method for rapidly detecting fluorescently-labeled allergens for hygienic products, and relates to the technical field of detection of hygienic products. The rapid fluorescently-labeled allergen detection method for the hygienic products comprises the following steps: combining a fluorescently-labeled substance with an allergen specific antibody through a chemical coupling method to form a stable fluorescently-labeled antibody compound; fixing the fluorescence labeled antibody on the surface of a reaction chamber of a micro-fluidic chip; introducing a hygienic product sample or an extract thereof into the chip, and combining the hygienic product sample or the extract thereof with the fixed fluorescently-labeled antibody to form an'allergen-antibody-fluorescently-labeled 'immune complex; a detection area is irradiated by an excitation light source, fluorescence intensity is monitored in real time by a photoelectric sensor, and a detection result is generated by combining a data analysis module. According to the invention, the rapid and high-sensitivity detection of allergens in hygienic products is realized by combining a fluorescence labeled antibody with a microfluidic technology.

The invention belongs to the technical field of immunology, and discloses a manganese salt adjuvant system based on dextran particle capsule shrinkage and a preparation method and application thereof.The manganese salt adjuvant system comprises beta-dextran particles GPs of a hollow spherical shell structure from yeast and manganese hydroxide colloid encapsulated in the dextran particles, the preparation method comprises the following steps: soaking the GPs in a MnCl2 solution, after the inner cavity of the GPs fully absorbs the MnCl2 solution, separating the GPs adsorbing MnCl2, then adding the GPs into a NaOH solution, forming stable manganese hydroxide colloid by manganese ions in the inner cavity of the GPs, and washing after separation to obtain the dextran particle encapsulated manganese salt adjuvant system. The GPMnOH particles carry various antigens at the same time in various modes such as electrostatic adsorption, chemical coupling or hydrophobic interaction, and an ideal carrier is provided for co-delivery immunization of the antigens and adjuvants.

The invention provides a hydrogen sensor as well as a preparation method and application thereof, and particularly relates to the technical field of sensors. The hydrogen sensor comprises a ceramic substrate electrode and a gas-sensitive layer, the gas-sensitive layer is arranged on the surface of the ceramic substrate electrode, the gas-sensitive layer comprises a composite material formed by doping a semiconductor material with precious metal, the surface of the composite material is coated with a hydrophobic membrane, and the surface of the hydrophobic membrane is coated with a metal layer. The hydrophobic membrane is formed by chemical coupling reaction of hydrophobic silane and hydroxyl groups. According to the hydrogen sensor, by adopting the specific semiconductor sensing material and the hydrophobic membrane, the adaptability and the stability of the hydrogen sensor in a high-temperature alkali liquor corrosion environment are greatly improved, the hydrogen sensor can reliably work for a long time in a severe environment, the replacement frequency of the sensor is reduced, and the use cost is reduced.

The invention discloses a pulverized coal selective oxygen group alkylation modification method, which starts from fire coal, and changes the molecular configuration of pulverized coal through a mechanical force chemical coupling alkylation reagent selective modification method, thereby influencing the migration and conversion path of fuel nitrogen in raw coal, realizing the directional regulation and control of the nitrogen-containing product of the fire coal, enhancing the homogeneous and heterogeneous reduction of NOx, and improving the combustion efficiency. And finally, ultra-low nitrogen emission of the fire coal is realized.

The application discloses a preparation method and application of a conjugate targeting pelvic autonomic nerves, wherein the conjugate is NE-RH formed by coupling of rhein and norepinephrine, the conjugate can realize precise targeting of norepinephrine neurons, improve the accumulation effect of RH in the norepinephrine neurons, and further enhance the nerve protection effect of RH. The conjugate has a molecular weight of less than 1000 Da, can help to penetrate the blood-brain barrier, and can avoid the defects of being easily removed by a mononuclear phagocyte system and having a short half-life. The production process of the conjugate is simple, the chemical coupling reaction is clear, the yield can reach 38%, and the purity of the product is greater than 99%, so that the conjugate is convenient to popularize and use. The conjugate and the preparation method have important significance for the damage repair of pelvic autonomic nerves.

The present application relates to the technical field of biotechnology, and particularly relates to a D-dimer chemiluminescence assay kit. The present application provides a D-dimer chemiluminescence assay kit, which comprises a magnetic particle suspension (immunomagnetic beads) coated with D-dimer antibody 1, alkaline phosphatase-labeled D-dimer antibody 2 (enzyme-labeled antibody) and a calibrant. The immunomagnetic beads and the enzyme-labeled antibody are prepared by chemical coupling and stored in a buffer containing a soluble high molecular material, which can effectively improve the detection sensitivity and repeatability. The kit can be used in combination with a substrate solution containing a chromogenic substrate such as adamantane and its derivative AMPPD or APS-5 for a full-automatic immune test system, and the content of D-dimer in plasma or whole blood can be determined by a chemiluminescence instrument. The kit has high detection sensitivity and good repeatability.

The embodiment of the application provides a kind of high temperature contact-corrosion coupling model construction method, determine the type of high temperature contact corrosion and corrosion internal mechanism;Build force-chemical coupling theory model;Solve the force-chemical coupling theory model using finite element algorithm;Based on the result obtained by solving, build high temperature contact-corrosion coupling finite element model.The scheme, based on irreversible thermodynamics, establishes a force-chemical full-coupling high-temperature oxidation corrosion theory model considering the interaction of contact pressure, reactant (gaseous) diffusion and chemical reaction;The high-temperature corrosion model is realized by finite element using the pre-set UEL module, which can accurately simulate the corrosion evolution law, corrosion morphology characteristics, chemical reaction dynamic process in the contact area during high-temperature contact corrosion process, and the change of contact pressure with reaction process.Meanwhile, it can also accurately present the distribution of stress, strain, contact pressure, reactant and product concentration and other physical fields.

The present invention relates to a chemical coupling linker, and an antibody-drug conjugate prepared with the linker. The present invention further relates to a use of the antibody-drug conjugate in treating related disease of tumors, etc.

The invention relates to a fly ash-based hierarchical pore molecular sieve composite material as well as a preparation method and application thereof, and belongs to the technical field of molecular sieve composite materials. In order to solve the problems that an existing fly ash based molecular sieve is single in pore structure, can only adsorb single pollutants and is complex in preparation process, the invention provides a preparation method of a fly ash based hierarchical pore molecular sieve composite material. The method comprises the following steps: carrying out mechanical-chemical coupling activation on fly ash, carrying out alkali fusion activation treatment, preparing a colloidal precursor, carrying out hydrothermal crystallization treatment, carrying out post-treatment on a crystallized product, and carrying out metal ion modification treatment. A micropore-mesopore-macropore three-level pore channel is constructed, the mass transfer efficiency and the adsorption activity are improved, double active sites are formed through metal modification, heavy metal and CO2 can be synchronously adsorbed, and the CO2 capacity is improved by 10-15% through heavy metal loading. The one-step method is adopted for preparation, the process is simplified, the cost is low, high consumption of fly ash is achieved, and the benefits of solid waste recycling and multi-pollutant cooperative treatment are achieved.

The invention relates to the technical field of new energy hydrogen production and chemical coupling application, and discloses a natural gas hydrogen-doped pipeline conveying system which comprises an energy supply layer, a chemical production layer, a digital integrated platform and a dispatching control module. The energy supply layer integrates photovoltaic power, wind power, nuclear power and a power grid standby power supply through a direct-current micro-grid, is matched with a sodium ion flow battery energy storage system, and supplies energy to the water electrolysis hydrogen production system of the non-noble metal catalyst and porous electrode structure. In the chemical production layer, pipeline steel or double-layer alloy steel is selected as a hydrogen-doped pipeline according to the proportion, a hydrogen concentration monitoring and sound wave leakage detection system is arranged, the hydrogen-doped safety upper limit of high pressure of 10% and low pressure of 30% is set, and a chemical hydrogen using system is connected through a gas distribution valve set and adjusted by an intelligent module. The digital platform is based on monitoring of the Internet of Things, hydrogen doping is optimized by means of a fuzzy PID algorithm and digital twinning, and risks are early warned. The scheduling module combines wind and light prediction and real-time electricity price, optimizes the start and stop of the electrolytic cell, the hydrogen doping ratio and the conveying pressure, and achieves efficient and stable conveying.

The invention discloses an aptamer sensor for detecting a novel psychoactive substance ketamine and application of the aptamer sensor. Belongs to the technical field of poison detection. The aptamer sensor for detecting the novel psychoactive substance ketamine is successfully established by taking a two-dimensional nano material as a fluorescence quencher and a carrier, chemically coupling the two-dimensional nano material with a double-chain nucleic acid aptamer through a liquid-phase amidation reaction and carrying out surface passivation treatment, so that the chemical coupling efficiency is greatly improved; and the stability and the sensitivity of the sensor are enhanced. In addition, when the sensor is introduced into complex biological matrixes such as blood, urine and saliva, the system does not display non-specific signals, and compared with a physical adsorption system, the KET in complex biological fluid can be reliably detected, and a new thought and method are provided for reliable detection of other novel psychoactive substances.

The invention discloses an astaxanthin liposome-coating probiotic compound system based on an interface free radical quenching and bidirectional coupling mechanism and application of the astaxanthin liposome-coating probiotic compound system in oxidative stress protection, and belongs to the technical field of biological medicines and probiotic preparations. According to the preparation method, probiotics with an iron ion mediated sodium alginate-guar gum coating are constructed, and the probiotics and astaxanthin nano-liposomes form an interface protection layer in a hydrogen peroxide stress microenvironment. Wherein astaxanthin protects the key enzyme activity of a thallus energy metabolism pathway, and the thallus continuously consumes a hydrogen peroxide substrate in the environment through an enzyme system of the thallus. According to the composite system disclosed by the invention, under the condition of no chemical coupling, synergistic enhancement of antioxidant defense and thallus activity can be realized through physical blending, the survival rate and colonization ability of probiotics in gastrointestinal stress are remarkably improved, and an innovative solution is provided for targeted intervention of inflammatory bowel diseases and development of functional probiotic preparations.

The invention belongs to the technical field of electrochemical synthesis and isotope labeling, and particularly discloses a method for preparing a deuterated compound through electrochemical coupling and a special catalyst thereof. According to the invention, a carbon-hydrogen bond halogenation-dehalogenation deuteration process is coupled, a specific noble metal nitrogen-doped carbon catalyst is screened and applied as a cathode and anode catalytic material, a brand new electrochemical system is constructed for the first time, and under the environment-friendly reaction conditions of completely no solvent and no supporting electrolyte, through electrochemical driving, the electrochemical performance of the electrochemical system is improved. The selective halogenation of carbon-hydrogen bonds of the anode and the dehalogenation deuteration reaction of the cathode are synchronously realized, the two half reactions are successfully and efficiently coupled in the same reactor, and the deuterated organic compound with high additional value is directly synthesized. According to the method, a large amount of organic solvents and electrolyte salts used in traditional organic synthesis are abandoned, the environmental load and post-treatment difficulty of the reaction are remarkably reduced, and the method has the outstanding advantages of being simple in process, environmentally friendly and high in atom economy.

The application discloses a kind of low reflection high adhesive photo surface treatment method, successively contain photo surface plasma treatment, UV film solution coating solidification, film outer surface plasma treatment etc., core is in UV film solution adding silane coupling agent type passivator;Photo surface uses atmospheric pressure plasma treatment, film outer surface uses low temperature plasma treatment, by controlling plasma treatment power, speed, working gas etc., realize the micro-nano rough structure construction of photo and film outer surface.The application utilizes double plasma treatment to realize physical roughening and reduce reflection, realizes the stability and chemical coupling of plasma activated surface by passivator, greatly improves the bonding strength of photo and film layer, and finally prepared film encapsulation photo film layer transmittance is greater than or equal to 90%, 60 degree glossiness is 10~30GU, while realizing low reflection effect without losing transparency, also has excellent wear resistance, bending performance.

The application belongs to the technical field of molecular imaging and nanobody of tumor diagnosis and treatment, and discloses a Trop2 targeted nanobody fluorescent probe and a preparation method and application thereof. The nanobody T4 or ABDT4 capable of specifically recognizing TROP2 is connected with near-infrared two-region fluorescent dye through a chemical coupling mode to obtain a nanobody fluorescent probe with TROP2 targeting capability. The application realizes selective recognition at a molecular level by introducing the nanobody capable of specifically recognizing TROP2, which is helpful to improve the fluorescent contrast between tumors and normal tissues. Stable probe products are obtained through a controllable coupling and purification process, which is beneficial to repeated preparation and subsequent popularization and application.

The invention relates to the technical field of lithium sulfide, and particularly provides a lithium sulfide material, a preparation method thereof and a solid-state battery. The lithium sulfide material comprises: a matrix, wherein the matrix is a lithium-containing crystalline sulfide; the shell layer coats at least part of the surface of the substrate, the shell layer comprises lithium halide, the transition layer is positioned between the shell layer and the substrate, the transition layer comprises a Li-S-X bond or a Li-(P / Si)-X bond, and X is halogen. According to the transition layer, chemical coupling of a matrix and a lithium halide shell layer is achieved on the atomic and chemical bond level, the adhesive force of the shell layer is enhanced, and the problem that interface chemistry and crystal lattices are not matched is solved; and meanwhile, the shell layer provides a transmission channel for lithium ions and plays a chemical passivation role on the surface of the matrix, so that migration of soluble sulfide and generation of related side reactions are inhibited, the solid-solid interface impedance is reduced through the structure, and the cycling stability, the rate capability and the environmental tolerance of the material are improved.

The invention provides a biotinylated Angiopep-2 modified targeting nano material as well as a preparation method and application of the biotinylated Angiopep-2 modified targeting nano material. According to the preparation method of the biotinylated Angiopep-2 modified targeting nano-material, Angiopep-2 is directionally anchored through a high-affinity biotin-streptavidin system, ligand conformation inactivation caused by chemical coupling is avoided, targeting activity is maintained, the used Angiopep-2 can help nanoparticles to be specifically recognized and combined and mediate the nanoparticles to cross a blood brain barrier to enter the brain, and the targeting nano-material can be used for preparing the targeted nano-material. The drug concentration in brain tissues is increased, so that the cure rate of brain diseases is improved, the brain targeting efficiency is far higher than that of transferrin and lactoferrin, and the application value is great; by entrapping curcumin and quercetin, a hydrophilic shell is provided, so that the water solubility of curcumin and quercetin is improved, and the bioavailability of curcumin and quercetin is improved.

The application discloses a kind of magnetic nanoparticles for atherosclerosis diagnosis and treatment.It is core-shell structure, the inner core is Fe₃O₄ magnetic nano material, shell is the drug-loading layer of biodegradable polymer material, the drug-loading layer is encapsulated with analog movement polypeptide K271la, and the surface of nano particle is covalently coupled with CD36 targeting antibody.Its preparation method mainly includes: by complex emulsification-solvent volatilization method, Fe₃O₄ nanoparticle and K271la polypeptide are co-encapsulated in one step, form nanoparticle, and then CD36 antibody is modified on the surface of particle by chemical coupling.The nanoparticle of the application has the ability of active targeting (CD36 antibody) and passive targeting (magnetic response), can be efficiently enriched in plaque, and deliver therapeutic polypeptide to macrophages, activate lipophagy to remove lipid;It can be used as magnetic resonance imaging contrast agent, realizes diagnosis and treatment integration, and provides a new strategy for solving the problem of low efficiency of atherosclerosis targeted therapy and lack of effective monitoring means.

The invention discloses a preparation method of a p-tau231 monoclonal antibody, the p-tau231 monoclonal antibody and an antibody variable region, and relates to the technical field of antibodies, the preparation method comprises the following steps: polypeptide design and synthesis: designing and synthesizing a phosphorylated polypeptide and a non-phosphorylated control polypeptide based on the 224th-238th amino acid sequence of tau protein, the phosphorylated polypeptide sequence is CKKVAVVRT (p) PPKSPSS, the phosphorylated polypeptide sequence is CKKVAVVRT (p) PPKSPSS, the phosphorylated polypeptide sequence is CKKVAVVRT (p) PPKSPSS, and the non-phosphorylated control polypeptide sequence is CKKVAVVRT (p) PPKSPSS; threonine (Thr231) at the 231st site is subjected to phosphorylation; the sequence of the non-phosphorylated polypeptide is CKKVAVVRTPPKSPSS, and the sequence of the non-phosphorylated polypeptide is Immunogen coupling: introducing cysteine residues to the N end of the phosphorylated polypeptide, and connecting the phosphorylated polypeptide with carrier protein KLH through a sulfydryl chemical coupling method to form an immunogen compound; and preparation of the conjugate for detection: introducing cysteine residues to the N ends of the phosphorylated polypeptide and the non-phosphorylated polypeptide, and respectively coupling with a carrier protein BSA to form the antigen complex for detection. The p-tau231 monoclonal antibody prepared by the technical scheme of the invention can be specifically combined with a p-tau231 antigen, has no specific reaction with non-phosphorylated polypeptide and tau polypeptide, and can be applied to early diagnosis of AD (Alzheimer's disease).