86 results about "Chemical groups" patented technology

A patch to be placed over an injury or defect in tissue in a biological structure having a lumen and preventing migration of contents of the biological structure through the injury or the defect. The patch includes an adhesive layer configured to adhere to the biological tissue via formation of hydrogen or covalent bonds with chemical groups present in the biological tissue and a backing layer coupled to and coextensive with the adhesive layer, the backing layer configured to be impervious to the contents.

The present invention discloses novel molecules consisting of N-methyl-D-aspartate receptor (NMDAR) antagonists-polymer conjugates having a general structure D-(X-Poly-T)n, wherein D is an high affinity, i.e., (+)-, (−)-, or (±)-dizocilpine, or a low affinity, i.e., (+)-, (−)-, or (±)-methadone, CNS active NMDAR antagonist, n is an integer comprised between 1 and 6. X is a stable (enzymatically and / or hydrolytically under physiological conditions) linker comprising a covalent bond or a chain of atoms that covalently attaches a small molecule NMDAR antagonist drug moiety to the Poly derivative. Poly is a covalently bonded chain of repeating monomer units that form a polymer or an oligomer backbone of synthetic or natural origin. T, if present, is either another molecule of D, or a terminal group of Poly, represented by any suitable chemical group which, depending upon preference, is unreactive or reactive with other chemical moieties, or has a targeting property.

The invention provides a sealing material for wet tissues as well as a preparation method and application of the sealing material. The sealing material comprises a hydrophobic oil phase and temperature-sensitive microspheres, the temperature-sensitive microspheres are dispersed in the hydrophobic oil phase to form a heterogeneous system; the temperature-sensitive microsphere is of a core-shell structure, and the core-shell structure comprises an inner core and an outer shell wrapping the inner core; the inner core comprises a temperature-sensitive cross-linked network and polysaccharide capable of being subjected to ionic cross-linking, and the shell is a porous shell body network and is formed by carrying out copolymerization reaction on a natural polymer derivative capable of being subjected to photo-crosslinking and a comonomer containing an active chemical group. The temperature-sensitive microspheres with core-shell structures are designed to cooperate with the hydrophobic oil phase, rapid hemostasis of dynamic and bleeding wet tissues is realized through a dual drainage mechanism of instantaneous drainage of the hydrophobic oil phase and active pumping of the temperature-sensitive inner core, and then stable chemical bonding is formed through cross-linking of the shell and tissue protein, so that the rapid hemostasis of the dynamic and bleeding wet tissues is realized. And the wet tissue is sealed.

The invention discloses a kit and a device for detecting acinetobacter baumannii. The kit comprises a first primer, a second primer, recombinase polymerase, Cas12a protein, crRNA and ssDNA, wherein the sequence of the first primer is as shown in SEQ ID NO.1; the sequence of the second primer is as shown in SEQ ID NO. 2; the sequence of the crRNA is as shown in SEQ ID NO.3, the crRNA is modified with a light-sensitive chemical group for preventing the combination of the crRNA and target nucleic acid, and the light-sensitive chemical group falls off from the crRNA when irradiated by light with a predetermined wavelength; the sequence of the ssDNA is as shown in SEQ ID NO.4, one end of the ssDNA is modified with a fluorophore, and the other end of the ssDNA is modified with a quenching group. By adopting the kit or the device provided by the invention, the acinetobacter baumannii can be accurately detected, the working efficiency can be improved, and the risk of cross contamination can be reduced.

An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.

The invention belongs to the technical field of fuel cells, and particularly relates to a gas diffusion layer structure with durability and high conductivity and a preparation method and application thereof. The prepared gas diffusion layer comprises a metal ion capture layer, a substrate layer and a microporous layer which are sequentially stacked, the metal ion capture layer contains a metal ion capture material, and the metal ion capture material is selected from one or more of polyethyleneimine, polyacrylic acid, polyvinyl alcohol, chitosan and a perfluorosulfonic acid ionomer; specifically, a metal ion capture layer is constructed on a substrate layer close to one side of a flow channel, the conductivity of the gas diffusion layer is improved by using a method of strong interaction force between a high electronegativity chemical group and metal ions, and meanwhile, the metal ions are efficiently captured in a broad-spectrum manner, so that the gas diffusion layer has a wide application prospect.

A patch to be placed over an injury or defect in tissue in a biological structure having a lumen and preventing migration of contents of the biological structure through the injury or the defect. The patch includes an adhesive layer configured to adhere to the biological tissue via formation of hydrogen or covalent bonds with chemical groups present in the biological tissue and a backing layer coupled to and coextensive with the adhesive layer, the backing layer configured to be impervious to the contents.

Nucleic acid enzymes such as DNAzymes are provided, including nucleic acid enzymes that can be used for detection or quantification of a cofactor, such as a metal ion, in a cell. Methods for concurrent detection of mRNA from cells are also provided and can benefit from modifications to the nucleic acid enzyme such as, e.g., inclusion of a polyadenine (poly-A) tail and / or nonnative chemical groups. The methods may utilize SNAIL probes for spatial transcriptomics, combining SNAIL probes with DNAzymes for spatial metallomics, e.g., to image metal ions spatially.

The instant invention concerns a process for fragmenting a polymer chain, comprising an oxidative degradation step wherein the polymer chain is contacted with a reduced form of a metal M and an oxidant such as dioxygen, and wherein the polymer carries covalently bound chemical groups acting as a ligand of said reduced form of the metal M.The invention also relates to methods making use of this fragmentation process as a first step of a degradation process of the polymer especially useful for avoiding the negative impact of a polymer to the environment. In this connection, the invention relates to the use of polymers carrying chemical groups acting as a ligand for a reduced form of a metal M, as a polymer biodegradable in an environment wherein the reduced form of a metal M is present.

The invention relates to a hemoglobin oxygen carrier based on small molecule modification as well as a preparation method and application thereof. The hemoglobin oxygen carrier comprises hemoglobin and N-fluoromethoxycarbonyl diphenylalanine, the hemoglobin forms the hemoglobin oxygen-carrying carrier through the non-covalent bond acting force between the hemoglobin and the N-fluoromethoxycarbonyl diphenylalanine. According to the hemoglobin oxygen carrier disclosed by the invention, a co-assembly strategy of N-fluoromethoxycarbonyl diphenylalanine and hemoglobin is rapidly implemented by taking a non-covalent bond acting force as a driving force. The hemoglobin oxygen-carrying carrier prepared by the process greatly shortens the stabilization time of hemoglobin molecules on the basis of ensuring the stability of molecular chemical groups, has good biocompatibility and injectable application characteristics on the basis of the characteristics of non-covalent acting force, and provides a new foundation for research and development of a new generation of hemoglobin oxygen-carrying carriers. And an engineering design thought and a theoretical basis are provided.

To provide a new specific SPECT radiopharmaceutical with high sensitivity for the detection of FAP protein expression in the tumour microenvironment, which is based on boronPro-type inhibitors (molecular-target radiopharmaceuticals).SOLUTION: Provided is a new fibroblast activation protein (iFAP) inhibitory radiopharmaceutical based on ((R)-1-((6-hydrazinylnicotinoyl)-D-alanyl)pyrrolidin-2-yl)boronic acid (HYNIC-iFAP), wherein ethylenediamine diacetic acid is used to complete the coordination sphere of the radiometal, combining the conventional use of HYNIC as a chelating agent for the radiometal 99mTc, and wherein the nitrogens of the hydrazine of HYNIC act as chemical groups that promote the interaction of the HYNIC-iFAP molecule with phenylalanine, glutamic acid, and serine in the active centre of fibroblast activation protein (FAP).SELECTED DRAWING: None

The invention discloses an ultrathin electromagnetic shielding layer based on a magnetron sputtering technology and a preparation method thereof, and the method comprises the steps: carrying out the oxygen plasma etching of the surface of a flexible substrate through a reactive ion etching technology, the reactive ion etching power ranges from 100 W to 140 W, so that an activated substrate surface chemical group is formed on the surface of the flexible substrate; a seed layer is formed on the etched surface of the flexible substrate in a sputtering mode through the magnetron sputtering technology, the thickness of the seed layer ranges from 1 nm to 100 nm, and the roughness of the seed layer is 10 nm or below; forming a layer of shielding metal film on the surface of the seed layer through sputtering by using a magnetron sputtering technology, wherein the thickness of the shielding metal film is less than 1 [mu] m; wherein the sputtering temperature in the magnetron sputtering process ranges from 100 DEG C to 150 DEG C.

The application discloses a blood glucose detection electrode, a nucleic acid detection electrode and a preparation method. The vertical graphene is grown by a PECVD method and has a three-dimensional petal-like structure. The vertical graphene under the structure is more solid than planar graphene. The vertical graphene can be used for grafting of chemical groups such as carboxyl groups, amino groups, aldehyde groups and hydroxyl groups in a plasma mode. The groups are introduced by the method, the structure and the conductivity of the vertical graphene are not damaged, the nucleic acid and the antibody are more favorable to occupy active sites, more nucleic acid and antibody are loaded, the electrochemical detection signal is improved, and in addition, the step of sealing can be omitted, and interference is avoided. The method can realize industrial production.

The present invention discloses a preparation method and application of a water repellent with self-recovering water repellency after washing, belonging to the field of environmentally friendly polymer technology. The present invention utilizes the unsaturated double bonds contained in the surface of modified titanium dioxide to undergo free radical polymerization with the acrylic ester monomers used in the synthesis of the water repellent, and the titanium dioxide and the main skeleton of the water repellent are connected by chemical groups, so that the titanium dioxide particles and the water repellent polymer are stably present in the water repellent emulsion, and the water repellent has high film fastness. The light-heat conversion ability of nano titanium dioxide and its contribution to the roughness of the fabric surface make the finished fabric have water repellency such as high static water contact angle, high hydrostatic pressure resistance, and high spray resistance. The fabric does not need to be dried after washing, and the spray resistance level can be effectively restored by conventional natural drying. At the same time, the ultraviolet shielding performance of nano titanium dioxide gives the fabric treated with the water repellent prepared by the present invention an anti-ultraviolet function.

The application relates to the technical field of organic synthesis, and discloses an acyl silicon reagent as well as a preparation method and application thereof. The acyl silicon reagent provided by the application has reducible chemical groups such as halogen and ester groups, can react with various amine compounds to generate amide compounds, has a wide application substrate range and high selectivity; the synthesis method of the amide compound can efficiently and highly selectively synthesize polypeptides, polymers and other amide compounds under the condition of anhydrous organic solvents or organic solvent aqueous solutions, and the yield of the amide compound is high.

The invention provides a compound, the general formula of which is A-L-B. In the general formula, A is a chemical group which specifically binds or activates STING (stimulating interferon gene) protein; b is a chemical group specifically combined with LC3 (microtubule-associated protein-1 light chain 3) protein; l is a chemical linker. The compound can effectively mediate the degradation of STING protein and is used for treating inflammatory diseases.

The application belongs to the technical field of organic optoelectronic material preparation and application, and discloses a boron-nitrogen heterocyclic derivative based on a fusion donor, preparation and application thereof.The organic material takes a boron-nitrogen heterocyclic ring containing different numbers of ring-forming boron atoms as a core, and is obtained by extending the molecular skeleton through the conjugation and ring of the peripheral chemical groups of the fusion donor.The boron-nitrogen heterocyclic derivative based on the fusion donor has excellent light and thermal stability, good electrochemical stability, and excellent electroluminescent performance, and can be applied to an organic electroluminescent device as a host material or a sensitizing agent material or a luminescent material, so that higher device efficiency, stability and color purity can be obtained.

This invention provides a method for protein functionalization involving post-translational modification in a manner that is selective, reliable, and under the possibility of benign, moderate redox, enabling the addition of reactive functional side chains. [Solution] The present invention relates to photoredox-mediated functionalization of proteins at chemical groups via radical generation and CC bond formation using specific boronate and sulfone precursor compounds. The present invention also relates to functionalized proteins that can be produced by this method, and the specific boronate and sulfone precursor compounds themselves.

A method for coating a substrate comprising at least one chemical group is provided by contacting the substrate surface with a compound comprising at least one carbon-carbon double bond or a mixture of a first monomer M1 and a second monomer M2. Then, a reaction is initiated by the reaction of the photoactive group and the carbon-carbon double bond by subjecting at least a portion of the formed complex to actinic radiation, wherein the wavelength of the actinic radiation is suitable for being absorbed by the photoactive group, to form a covalent bond. The method is largely resistant to inhibition by impurities in the substrate surface. Greater uniformity of the elastic modulus of the entire substrate and the added layer can be achieved. When an additional topcoat is applied, the resulting surface has high hardness, and the scratch resistance is improved. When a top sheet is applied, the abrasion resistance is improved.

The application discloses a kind of multicolor fluorescent emission lignin-based carbon dots and preparation method and application thereof.The preparation method of the multicolor fluorescent emission lignin-based carbon dots includes the following steps: (1) lignin and organic acid are added to water, microwave treatment is carried out, and A carbon dots are obtained;(2) A carbon dots and oxidizing agent are added to water to carry out oxidation reaction, and B carbon dots are obtained;(3) B carbon dots are added to water, and esterification reaction is carried out by adding alcohol compound and acidic catalyst, and C carbon dots are obtained;(4) C carbon dots and reducing agent are added to water in proportion to carry out reduction reaction, and D carbon dots are obtained;(5) D carbon dots and alkaline catalyst are added to water, and alkaline hydrolysis reaction is carried out, and the multicolor fluorescent emission lignin-based carbon dots are obtained.The lignin-based carbon dots prepared by the application can realize blue, green, yellow and red fluorescent emission by microwave digestion treatment and surface chemical group regulation method.

The invention discloses a silicon nanowire water photovoltaic device, a preparation method and a water photovoltaic cell. The silicon nanowire water photovoltaic device comprises an n-type silicon nanowire layer, a top electrode arranged on the top surface of the n-type silicon nanowire layer and a bottom electrode arranged on the bottom surface of the n-type silicon nanowire layer, wherein Si-OH groups on the surface of the n-type silicon nanowire layer are at least partially substituted by chemical bonds or chemical groups with the electron withdrawing capability weaker than that of the Si-OH groups. According to the silicon nanowire water photovoltaic device, the preparation method and the water photovoltaic cell provided by the invention, Si-OH groups on the surfaces of the silicon nanowires are at least partially replaced by chemical bonds or chemical groups with relatively weak electron attraction capability, so that the number of free electrons in the silicon nanowires can be remarkably increased through the replacement; and when microcirculation caused by water evaporation enables a charge carrier in the silicon nanowire to move directionally, more electrons can participate in the process, so that the hydrovolt effect is enhanced, and the electrical property of the silicon nanowire hydrovolt device is remarkably improved.

The application provides a sealing material for wet tissues, a preparation method and application thereof, and the sealing material comprises a hydrophobic oil phase and temperature-sensitive microspheres; the temperature-sensitive microspheres are dispersed in the hydrophobic oil phase to form a heterogeneous system; the temperature-sensitive microspheres have a core-shell structure, the core-shell structure comprises a core and a shell covering the core; the core comprises a temperature-sensitive cross-linked network and an ion-cross-linkable polysaccharide, and the shell is a porous shell body network and is formed by a copolymerization reaction of a light-cross-linkable natural polymer derivative and a co-monomer containing an active chemical group. The application realizes rapid hemostasis on dynamic and bleeding wet tissues through a double drainage mechanism of the hydrophobic oil phase instantaneously draining liquid and the temperature-sensitive core actively pumping, and realizes sealing on the wet tissues through the shell and tissue protein cross-linking to form stable chemical adhesion.

The invention discloses a hydrogel complexing agent and application thereof in separation of main protein of royal jelly. The hydrogel complexing agent comprises a poly (N-isopropylacrylamide) hydrogel matrix, a network topology structure is formed in the poly (N-isopropylacrylamide) hydrogel, and the pore diameter of pores of the network topology structure is 18-25 nm. A network topological structure with holes of 18-25 nm is formed in the hydrogel complexing agent, and the network topological structure is matched with a three-dimensional structure of the royal jelly major protein, so that the hydrogel complexing agent can be used as a royal jelly major protein trap; the network topology structure is convenient for the royal jelly main protein to enter the holes, and the chemical groups in the holes can form supramolecular interaction with the royal jelly main protein, so that the capture of the hydrogel complexing agent on the royal jelly main protein is realized, and a simple, low-cost and high-yield method is provided for separating the main protein from the royal jelly.

The present application provides methods of functionalizing an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal or by culturing an organ or tissue in a bioreactor containing such nutrient. The present application also provides methods of selectively functionalizing extracellular matrix (ECM) of an organ or tissue of a mammal by administering a nutrient (e.g., peracetylated N-azido galactosamine Ac4GalNAz) to the mammal. In some aspects, the present application provides a decellularized scaffold of a mammalian organ or tissue comprising an extracellular matrix, wherein the extracellular matrix of the decellularized scaffold is functionalized with a chemical group that is reactive in a bioorthogonal chemical reaction, such as an azide chemical group. The present application also provides biological prosthetic mesh and mammalian organs and tissues for transplantation prepared according to the methods of the application.

This invention provides methods for producing a polymer particle which contains unusually high negative charges on the surface of the particle. Preferably, the polymer is pharmaceutically acceptable. The negative charges can be conferred by chemical groups such as carboxyl, sulfonate, nitrate, fluorate, chloride, iodide, persulfate, and many others, with carboxyl group being preferred. The invention also provides polymer particle produced by the methods of the invention.

The invention discloses extracellular matrix mimetic peptide modified silk fibroin hydrogel as well as a preparation method and application thereof in organoid culture. The preparation method of the extracellular matrix mimetic peptide modified silk fibroin hydrogel comprises the following steps: S1, preparing an extracellular matrix ECM mimetic peptide modified by a click chemical group; and S2, preparing the ECM mimic peptide modified silk fibroin hydrogel by utilizing a click chemical means. Compared with a conventional organoid culture system, the preparation method of the silk fibroin hydrogel modified by the extracellular matrix mimic peptide, provided by the invention, can orthogonally regulate and control the rigidity, the ligand type and the ligand density of the hydrogel, and provides a personalized and customizable extracellular matrix environment for three-dimensional culture and organoid culture of cells.

The present invention relates to the field of producing protein conjugates. The invention relates to a novel method for producing a protein conjugate comprising a protein of interest (POI) bearing a chemical moiety and a payload comprising a complementary chemical moiety. The said method comprises the extracellular expression of the POI, contacting the POI in the cell culture supernatant with the payload, allowing the formation of a covalent bond between the POI and the payload in a cell culture supernatant, thereby creating a protein conjugate, and purifying said conjugate.

The invention relates to a preparation method and application of sugar-responsive protamine insulin, which comprises the following steps: modifying a chemical group on cysteine of protamine, connecting with a phenylboronic acid compound to form a compound, and mixing with insulin to prepare the sugar-responsive protamine insulin. The sugar-responsive insulin preparation can realize an intelligent sugar-responsive regulation mechanism. Specifically, the insulin can be slowly released at a normal blood glucose level and is accelerated to be released at a hyperglycemia state, so that the blood glucose is effectively regulated and controlled, and the occurrence of hypoglycemia is avoided.

The invention discloses a hemostatic sponge preparation method, which comprises: S1, dissolving methacrylic anhydride modified gelatin in deionized water to obtain a methacrylic acid esterified gelatin solution; s2, dissolving methacrylic anhydride modified methyl cellulose in deionized water to obtain a methacrylic acid esterified methyl cellulose solution; s3, mixing the metacrylic acid esterified gelatin solution and the metacrylic acid esterified methyl cellulose solution, and then adding a photoinitiator for photo-crosslinking to construct hydrogel with a multi-network structure; s4, the hydrogel is placed in a freeze-drying machine for freeze-drying treatment, and the hemostatic sponge of a porous structure is formed; the invention also discloses the hemostatic sponge prepared by the preparation method of the hemostatic sponge, the hemostatic sponge prepared by the preparation method of the hemostatic sponge provided by the invention has good degradability, and hydrogel with a multi-network structure is formed by introducing a double-bond chemical group and utilizing photo-crosslinking. The mechanical property and the water absorption performance of the material are remarkably improved, and platelet adhesion and hemostasis promotion are facilitated.