2052 results about "Bacteriocides" patented technology

The invention belongs to the technical field of medicinal chemistry, and particularly relates to quinolinone compounds, a preparation method and application of the quinolinone compounds in prevention and treatment of plant diseases. Compared with the prior art, the quinolinone compound is synthesized and applied to plant disease control, and the technical problem that the bacterial disease treatment effect of a chemical bactericide is reduced due to the fact that the resistance of original bacteria to the bactericide is enhanced in the plant bacterial disease treatment process is solved.

The invention relates to the technical field of daily chemical products, in particular to a scalp care composition and application thereof. From the perspective of scalp care, selenium disulfide and piroctone olamine salt are taken as bactericides and compounded with bactericidal and anti-dandruff components to form the organic and integrated anti-dandruff and scalp conditioning composition, and the composition has the effects of removing dandruff, relieving itching, soothing and controlling oil and can effectively relieve the problems of dry dandruff and fungal dandruff. The scalp care composition is added into the shampoo, serves as a main component of the multi-effect repairing and dandruff removing shampoo, can effectively solve the problems of oily dandruff, dry dandruff, scalp itching, scalp hair greasy, scalp sub-health and the like, and has the effects of reducing scalp inflammatory response, promoting updating of scalp cutin, inhibiting generation of scalp redundant sebum and the like; the nursing dimension is more comprehensive, and the application range is wider.

The present invention relates to silica aerogels with a high to very high loading (60-90% w / w) of encapsulated biocidal and / or biorepellant compounds and to methods of making and using such aerogels in anti-fouling compositions, which are especially suitable for marine use.

The invention discloses an inorganic modified super-long salt-spray-resistant water-based paint and a preparation method thereof, and relates to the technical field of water-based industrial paints. The inorganic modified super-long salt-spray-resistant water-based paint at least comprises the following raw materials in parts by mass: 60-70 parts of water-based acrylic resin, 0.1-0.5 part of a bactericide, 0.2-0.4 part of a wetting agent, 0.5-1 part of a dispersing agent, 0.1-0.3 part of a defoaming agent, 0.5-1 part of a pH regulator, 8-12 parts of nano silicon dioxide coated polymerized calcium phosphate, 0.5-2 parts of modified zinc phosphate, 8-10 parts of silk mica powder, 2-4 parts of a coalescing agent and 10-20 parts of water. And 0.5 to 1 part of a polyurethane thickening agent. The inorganic modified super-long salt-fog-resistant water-based paint prepared by the invention has super-long salt fog resistance, high base material adhesion and low ion dissolution amount, and the hardness and water resistance of a coating are also improved.

The invention provides a pharmaceutical composition containing a pyrazole ether compound and application of the pharmaceutical composition, the pharmaceutical composition comprises an effective component A and an effective component B. The effective component A is a pyrazole ether compound with a structure shown in a formula I; and the effective component B is selected from any one or a combination of at least two of azole bactericides, mitochondrial electron transport chain compound III inhibitor bactericides, SDHI bactericides, multi-site contact type bactericides or other bactericides. In the invention, the effective component A and the effective component B have a synergistic effect, so that the pharmaceutical composition has higher biological activity and favorable or wider active spectrum, can reduce the application amount of compounds and compositions required for effectively controlling phytopathogens, and has longer lasting activity.

The invention relates to the technical field of cutting fluids, and discloses a high-temperature alloy cutting fluid and a preparation method thereof, the high-temperature alloy cutting fluid comprises the following components: 30%-50% of base oil, 5%-10% of organic alkali, 5%-10% of an antirust agent, 4%-10% of synthetic ester, 15%-20% of an anti-wear reagent at extreme pressure, 0.5%-1.5% of a corrosion inhibitor, 7%-15% of an emulsifier, 2%-4% of a bactericide, 0.05%-0.1% of a defoaming agent, and the balance of water; the extreme pressure anti-wear agent is composed of a chlorination extreme pressure anti-wear agent and a vulcanization extreme pressure anti-wear agent. According to the invention, the ester-based base oil, the organic alkali, the synthetic ester and the extreme pressure anti-wear agent composed of the chlorination extreme pressure anti-wear agent and the vulcanization extreme pressure anti-wear agent are combined to form a continuous high-strength lubricating film, and the continuous high-strength lubricating film is compounded with additives such as the organic acid antirust agent, the corrosion inhibitor and the bactericide; therefore, the antirust corrosion inhibition performance of the cutting fluid on workpieces and machine tools and the long-term use safety and environmental protection performance are improved during processing.

The invention provides bacillus pseudomycoides BP88 and application of the bacillus pseudomycoides BP88. The preservation number of the bacillus pseudomycoides BP88 is CCTCC (China Center For Type Culture Collection) NO.20241800. The invention further provides a preparation method of the bacillus pseudomycoides BP88. The bacillus pseudomycoides has a good disease prevention effect on tobacco root rot and has a remarkable growth promoting effect on tobacco plants. The strain has good theoretical research value and application prospect, can be used as a good test material for researching interaction and action mechanism of soil bacteria and plants, can also be used for research and development of soil bio-fertilizers and biological bactericides, can also become a good carrier of multifunctional engineering bacteria for disease prevention, growth promotion and the like, and has broad application prospects. And a new strain resource is provided for biological prevention and control of tobacco root rot.

The present disclosure relates to a fungicidal composition. More particularly, the present disclosure relates to a granular fungicidal composition comprising a copper compound and a benzamide fungicide. The present disclosure also relates to a process for preparing the fungicidal composition and a method for controlling growth of fungal phytopathogens using the same.

The invention relates to the technical field of fracturing fluids, and particularly discloses a high-performance water-based fracturing fluid and a preparation method thereof. A preparation method of a high-performance water-based fracturing fluid comprises the following steps: 1) dissolving a compound as shown in a formula I in a solvent, then adding a cyclodextrin derivative and hydroxyproline, mixing, then adding tetrabutylphosphorus hydroxide, and continuously and uniformly mixing to prepare a precursor solution; (2) adding a compound as shown in a formula II into the precursor solution, mixing, reacting, then adding maleimide or a maleimide derivative, heating to 60-70 DEG C, and continuously reacting for 2.5-4 hours to obtain a precursor; and 3) uniformly mixing water and guanidine gum to prepare a base solution, adding the cross-linking agent, the precursor, the clay stabilizer, the gel breaker, the bactericide and the pH regulator in a stirring state, and uniformly mixing to obtain the high-performance water-based fracturing fluid. The prepared high-performance water-based fracturing fluid has the advantages of thorough gel breaking and small solid-phase damage.

The invention discloses a pesticide slow-release granule suitable for simultaneous sowing of pesticide and plant seeds. The pesticide sustained-release granule has double coating layers, the first coating layer takes a pesticide effective component A as a core and a nano calcium carbonate carrier material as a shell, and nano calcium carbonate is used for carrying out first-layer coating on a bactericide effective component through a physical adsorption method; the second coating layer is formed by mixing the first coating layer and the pesticide active ingredient B as a core and taking materials such as attapulgite as a shell. The sustained-release granule has good sustained-release performance, can stably release effective components in a long time, prolongs the pesticide effect period, reduces frequent pesticide application operation, and further improves the pesticide utilization rate.

The invention relates to the technical field of sewage treatment, in particular to a CCRO high-efficiency water treatment system and a working method thereof. Comprising a sewage tank, a water supply pump, a scale inhibitor injection module, a sodium hydrogen sulfite injection module, a bactericide injection module, a security filter, a static mixer, a high-pressure pump, a reverse osmosis membrane assembly, a reaction tank, a circulating pump, a cleaning pump, a water production tank and a concentration device which are connected through pipelines, the sewage tank, the water supply pump, the security filter, the static mixer, the high-pressure pump, the reverse osmosis membrane assembly, the reaction tank, the circulating pump, the cleaning pump and the water production tank are all provided with an outlet and an inlet, and the reverse osmosis membrane assembly is also provided with a drain outlet; the sewage pool, the water supply pump, the static mixer, the security filter, the high-pressure pump, the reverse osmosis membrane assembly and the water producing pool are sequentially connected through the outlet and the inlet through pipelines; the problem that an existing CCRO water treatment system is insufficient in automation degree can be effectively solved, the sewage treatment efficiency and quality are improved, the operation and maintenance cost is reduced, and wide application prospects are achieved.

The invention provides a fluopicolide preparation method and a fluopicolide preparation device, and relates to the technical field of pesticide bactericide production. A first feeding pipe and a second feeding pipe are mounted at the left end of the first reaction tank, and the right end of the first reaction tank is connected with a second reaction tank through a first discharging pipe; raw materials participating in the reaction are cheap and easy to obtain, the product quality and yield stability are high, the reaction conditions are mild, and the device and the preparation method are suitable for large-scale production in workshops; when the device discharges, powder adhered to the inner wall of the drying tank is automatically scraped and cleaned, so that the device has better cleaning and using effects and better maintenance efficiency; the problems that multiple chemical substances need to be introduced to participate in the reaction, the reaction process is complex, later impurity removal and purification are not facilitated, the production cost is relatively high, the reaction speed is slow, the reaction effect is poor, and the device and the preparation method are not suitable for large-scale production in workshops are solved.

Mobile or stationary air filter (L) or room fan (RL) according to Figure 1, comprising an outer wall (5), a (2) for generating an airflow (3) which is guided through an air filter material (1.1), an air inlet (2.1) and an air outlet (2.2), wherein the air filter material (1.1) contains poly(styrenesulfonic acid) (sulfonic acid biocide) (1.3) cross-linked with divinylbenzene and / or an acidic salt (1.3.1) (sulfonic acid biocide salt) of the sulfonic acid biocide (1.3) with biocidal cations, wherein the sulfonic acid biocide (1.3) and / or sulfonic acid biocide salt (1.3.1)(i) are positively and / or materially bonded to and / or in inert carriers and, in the fixed state, are in direct contact with carrier materials (1.1.7) on and / or in which biocidal Metal microparticles of a mean particle size d50 of 0.1 µm to <1,000 µm are fixed, and / or (ii) with carrier materials (1.1.7) on and / or in which biocidal metal microparticles of a mean particle size d50 of 0.1 µm to <1.000 µm are fixed, are mixed; its use and methods for the production and environmentally friendly disposal of air filter materials (1.1).

This invention relates to the field of water treatment technology, specifically to a reverse osmosis membrane cleaning agent composed of a non-oxidizing bactericide and an enzyme, and its application. Its main purpose is to remove biofouling from reverse osmosis membranes and restore membrane flux. This patent discloses a novel reverse osmosis membrane cleaning agent formulation. Considering the different pH requirements of the cleaning agent in actual production, different types of bioactive hydrolytic enzymes are selected to disrupt the structure of bacterial cell walls and their extracellular polymers. A reducing agent and a non-oxidizing bactericide are combined to enhance the cleaning effect of the reverse osmosis membrane. For different cleaning purposes, non-oxidizing bactericides suitable for acidic and alkaline cleaning are formulated. Sodium benzoate and benzalkonium chloride show good application prospects in the removal of reverse osmosis membrane fouling.

The invention discloses an application of an sscle13g095080 gene and a protein coded by the sscle13g095080 gene in regulation and control of pathogenicity of sclerotinia sclerotiorum. The nucleotide sequence of the sscle13g095080 gene is as shown in SEQ ID NO: 1 (Sequence Identity Number 1). The invention discovers that the sclerotinia sclerotiorum sscle13g095080 gene is related to the pathogenicity of sclerotinia sclerotiorum, and the pathogenicity of sclerotinia sclerotiorum can be obviously reduced by knocking out the sclerotinia sclerotiorum sscle13g095080 gene Therefore, the sscle13g095080 gene can be used as an effective target for preventing and treating the sclerotiniose, compounds capable of preventing gene expression and protein expression, modification and positioning of the gene can be screened, occurrence of the sclerotiniose can be effectively controlled, and development of novel bactericides is facilitated.

The invention relates to a sterilizing, corrosion-inhibiting and scale-inhibiting foaming agent for foam drainage and a preparation method thereof, and belongs to the technical field of oil and gas development. The sterilizing, corrosion-inhibiting and scale-inhibiting foaming agent comprises the following components in percentage by mass: 1%-2% of a triazole bactericide, 3%-5% of a scale inhibitor, 0.2%-1% of polyvinyl alcohol, 10%-18% of a bis-imidazoline sulfonated quaternary ammonium salt zwitterionic surfactant, 5%-10% of benzene sulfonate, 5%-10% of Ninol and the balance of water. The foaming agent is good in foaming performance, the prepared foam liquid is stable in foam, very high in liquid carrying capacity and good in salt resistance, temperature resistance and oil resistance, compared with a foaming agent in the prior art, the foaming agent is better in performance, and the foaming agent is suitable for different oil and gas wells, shale gas wells and other environments. And besides foaming performance, the detergent also has excellent sterilization, corrosion inhibition and scale inhibition effects, realizes multiple effects by one agent, and is very strong in comprehensive performance.

The invention provides an insecticidal pesticide synergistic additive containing plant essential oil and a preparation method thereof, and belongs to the technical field of pesticide synergists. The insecticidal pesticide synergistic aid containing the plant essential oil comprises a synergist matrix, a penetrating agent, an emulsifying agent, a thickening agent, an anti-freezing agent and the plant essential oil. The main component of the synergistic additive is esterified vegetable oil or fatty acid alkyl ester, and the synergistic additive has the advantages of low toxicity, difficult generation of drug resistance of target organisms, environmental friendliness and the like, and can be mixed with a penetrant, an emulsifier and a thickener for use, so that the wetting and infiltration capacity of pesticide liquid can be remarkably enhanced, a wax layer structure can be destroyed, the permeability of the pesticide liquid can be improved, and the pesticide effect can be improved. The added plant essential oil belongs to a natural active substance, has the characteristics of being green and environment-friendly, and can be matched with different pesticide formulations such as insecticides, bactericides, herbicides and the like for use.

The invention provides an environment-friendly self-repairing single-component waterborne polyurethane waterproof coating and a preparation method thereof. The environment-friendly self-repairing single-component waterborne polyurethane waterproof coating is prepared from the following components in percentage by mass: 35 to 45 percent of waterborne hyperbranched polyurethane resin, 10 to 15 percent of titanium dioxide, 15 to 20 percent of barium sulfate, 5 to 10 percent of modified quartz powder, 0.1 to 1 percent of a thickening agent, 0.2 to 0.3 percent of a sterilizing agent, 3.5 to 6.5 percent of an auxiliary agent and the balance of water. The waterproof coating contains certain fluorine atoms, so that a paint film has relatively stable long-acting hydrophobic performance after being dried, and the paint film can be kept clean and self-cleaning for a long time; in addition, the modified quartz powder has the effect of enhancing the mechanical property and stability of the paint film; and meanwhile, the paint film forms a compact network structure in the drying process, so that the paint film has relatively good flexibility and relatively high hardness. In addition, the components contain more active groups, so that the paint film can be self-repaired.

The invention discloses a glass fiber with high strength and strong tensile property and a manufacturing method thereof, the glass fiber is composed of 10-15 parts by weight of pyrophyllite, 10-15 parts by weight of quartz sand, 10-15 parts by weight of limestone, 10-15 parts by weight of dolomite, 10-15 parts by weight of borocalcite, 10-15 parts by weight of szaibelyite, 10-15 parts by weight of bactericide, 10-15 parts by weight of anti-ultraviolet agent, 10-15 parts by weight of graphene, 10-15 parts by weight of Kevlar fiber, 10-15 parts by weight of PBO fiber, 10-15 parts by weight of titanium dioxide and 10-15 parts 8-12 parts of quartz sand; 3-8 parts of limestone; 4-8 parts of dolomite; 10 to 18 parts of borocalcite; 11 to 17 parts of szaibelyite; 2-6 parts of a bactericide; and 3-8 parts of an anti-ultraviolet agent. The titanium dioxide and the aluminum oxide are added into the raw materials, the overall strength of the prepared glass fiber can be effectively improved, the graphene, the Kevlar fiber and the PBO fiber are added into the raw materials, the overall tensile property of the prepared glass fiber can be effectively improved, and the bactericide is added into the raw materials, so that the service life of the glass fiber is prolonged. By adding the anti-ultraviolet agent into the raw materials, the overall antibacterial property of the prepared glass fiber can be effectively improved, and the overall anti-ultraviolet property of the prepared glass fiber can be effectively improved by adding the anti-ultraviolet agent into the raw materials.

The invention discloses application of phloroglucinol compounds to inhibition of fusarium plant pathogenic fungi and prevention and treatment of related plant diseases, and belongs to the technical field of agricultural bactericides. In order to solve the technical problems that an existing method for preventing and treating plant diseases caused by fusarium is poor in effect and has defects, 15 compounds are extracted from dryopteris fragrans, and structural identification shows that the 15 compounds belong to phloroglucinol compounds. Then, the in-vitro bacteriostatic effects of the 15 compounds on fusarium oxysporum, fusarium graminearum and fusarium laminatum are detected, the compounds 5, 6, 7, 8, 10, 11, 13 and 14 are found to have bacteriostatic effects on fusarium fungi, and the compound 10 (aspidinol B) has the strongest in-vitro inhibitory effect on the three fusarium fungi; furthermore, an in-vivo antibacterial experiment proves that the compound 10 has a good prevention and treatment effect on pathogenic fungal diseases of three fusarium plants.

The invention belongs to the technical field of novel pesticides, and particularly provides a bactericide suspending agent and a preparation method thereof.The effective component of the bactericide suspending agent is prothioconazole, and on the basis of the effective component of the bactericide suspending agent, microencapsulated oxidized vegetable oil accounting for 5-15% of the weight of the whole bactericide is added to serve as a synergistic auxiliary agent. The prothioconazole bactericide has the advantages that the adhesion capacity of the prothioconazole bactericide on the surfaces of crops can be obviously improved, the adverse effects caused by environmental factors such as rain wash are reduced, and the absorption of the bactericide is facilitated, so that the synergistic effect is achieved.

The invention relates to the technical field of agricultural planting, in particular to a method for preventing and treating watermelon fusarium wilt by using a green bactericide, which comprises the following steps: preparing the green bactericide which comprises the following components in percentage by mass: 18-25% of composite plant source active component consisting of water lettuce extract, enteromorpha polysaccharide and field horsetail flavone; 20%-30% of an antagonistic bacterium compound preparation, wherein the antagonistic bacterium compound preparation comprises bacillus pumilus and trichoderma; 38%-45% of a nano-carrier, wherein a chitosan-sodium alginate compound is used as an embedding material; and the balance of auxiliary components. Applying the green bactericide to watermelon plants; through component optimization and a synergistic effect, compared with the prior art, the composition has the remarkable advantages that the safety is high, all the components are from natural plants or microorganisms, no chemical pesticide residue exists, the composition is healthy to human bodies and environmentally friendly, and the problems of drug resistance and soil pollution caused by chemical agents can be avoided.

The invention relates to a biological long-acting bactericide and a preparation method and application thereof.The biological long-acting bactericide can release a quaternary ammonium salt type sophorolipid sterilization unit with the sterilization effect according to needs, the sterilization time is effectively prolonged, the recycling performance of the bactericide is improved, and the biotoxicity of the bactericide is reduced; the excessive use of the bactericide is avoided to a great extent; in addition, the biological long-acting bactericide also has excellent substrate adhesion and washing resistance, and is beneficial to inhibition of adhesion of bacteria on the surface of a material, so that the surface of the material washed by water still keeps relatively high antibacterial ability.

The invention discloses an E3 ubiquitin ligase gene ulA, the nucleotide sequence of which is shown as SEQ ID NO: 1. The gene is knocked out from fusarium oxysporum by using a homologous recombination method to obtain a mutant strain ulA; compared with a wild strain, the mutant hulA has the advantages that the spore yield is reduced, the spore germination is delayed, the response capability to various abiotic stresses is reduced, the pathogenicity to panax notoginseng plants is reduced, and the sensitivity to six bactericides is increased, and experimental results show that the gene plays an important role in pathogenicity, drug resistance and the like of fusarium oxysporum. The gene can be used as a target gene for developing an anti-fusarium oxysporum preparation.

The invention provides an intelligent carbon source adding method and system for sewage treatment and a storage medium, and relates to the technical field of intelligent planning. The carbon source adding process is accurately regulated and controlled by comprehensively analyzing the action effect of target heavy metal and target bactericide and utilizing regression and optimization technologies; meanwhile, an intelligent neutralizing agent and carbon source adjustment strategy is provided by combining actual error judgment, so that the maximization of denitrification efficiency and the optimal configuration of system resources are realized; the precision and stability of carbon source feeding are improved, and a technical foundation is laid for long-term sustainable operation of sewage treatment.

The invention discloses a method for jointly degrading a dicarboximide bactericide through a bacterium-enzyme medium system. According to the method, Pseudomonas sp. F13, laccase and a laccase medium are adopted for jointly degrading the dicarboximide bactericide and metabolites of the dicarboximide bactericide. The preservation number of the pseudoarthrobacter sp. F13 in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M 20241866, and the preservation number of the pseudoarthrobacter sp. F13 in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M 20241866. Experimental results show that under the condition that the pH is 4.8 and 6.0, 100% degradation of the dicarboximide fungicide can be achieved through the method, accumulation of 3, 5-DCA is not detected, the method provides a new way for degradation of procymidone and toxic intermediate metabolite 3, 5-DCA, and the method is simple, easy to operate and suitable for market popularization and utilization.

The present invention relates to the technical field of inorganic multi-color coatings, and specifically relates to a preparation method and apparatus for an inorganic multi-color coating, and a use. The preparation method comprises the following raw materials: base paint: water, cellulose, a defoaming agent, a dispersing agent, a wetting agent, a luminescent material, an inorganic hybrid emulsion, an odorless pH regulator, and a bactericide; granulation liquid: water, a bactericide, xanthan gum, protective colloid powder, an odorless pH regulator, nanocellulose, and a defoaming agent; and a continuous phase emulsion: an inorganic hybrid emulsion, a bactericide, inorganic gel, deionized water, an odorless pH regulator, a thickening agent, and a defoaming agent. In the present invention, a mixture of CaAl2O4 calcium aluminate and BaAlO4 barium aluminate is added to serve as a luminescent material, the luminescent material can absorb and store solar energy by means of a specific mode when exposed to sunlight during the daytime, and at night, the internally stored energy is converted into optical radiation to be released, thereby realizing a light-emitting effect and realizing the luminous function of the coating.

The invention relates to the technical field of sewage treatment, in particular to a composition for degrading triazole bactericides and application of the composition. The invention provides a composition for degrading triazole bactericides. The composition comprises persulfate and a catalyst, the catalyst takes foamed nickel as a carrier and is loaded with a cobalt-nickel layered bimetallic oxide. The cobalt-nickel layered bimetallic oxide is rich in oxygen vacancies, and has a larger specific surface area, higher stability and a higher oxidation reduction rate. In the process of activating sulfate by the cobalt-nickel layered bimetallic oxide, a large amount of free radical sulfate free radical # imgabs0 # superoxide free radical # imgabs1 # and non-free radical singlet oxygen (1O2) are generated, and triazole bactericides are synergistically degraded based on a free radical path and a non-free radical path.

The invention belongs to the technical field of fine chemical engineering, and particularly discloses a method for preparing a broad-spectrum bactericide 2-n-butylbenzo [d] isothiazole-3 (2H)-ketone by a molecular editing method. The preparation method comprises the following specific steps: taking 3H-1, 2-benzodisulfophenol-3-ketone and n-butylamine as raw materials, taking Selectfluor as an additive, adding the raw materials and the additive into a mixed solvent, and carrying out heating and stirring reaction, so as to obtain the target product 2-n-butylbenzo [d] isothiazole-3 (2H)-ketone (BBIT). The method is simple and convenient to operate, the raw materials are easy to obtain, the yield is high, thiophenol raw materials or excessive alkali are prevented from being used, and potential application value is achieved.

The invention discloses a high-survival-rate grafting method for grafting pinus sylvestris to pinus koraiensis in arid regions, which comprises the following steps: selecting pinus sylvestris stock with the growth period of 4-6 years, flatly cutting below a terminal bud branch point to form a cross section, downwards and obliquely cutting twice from the cross section to form a triangular pyramid-shaped chimeric structure, cutting a semi-lignified pinus koraiensis scion with a terminal bud, and grafting the semi-lignified pinus koraiensis scion with the terminal bud to obtain the pinus sylvestris scion. After main buds are reserved and lateral buds are cut off, two oblique triangular xylem sections are obliquely cut out from the lower portion of spores through two cutters, and a double-slope wedge-shaped joint body is formed; when the rootstock and the scion are cut, the cutting blade is immersed in an aqueous solution containing a plant growth regulator and a bactericide; the double-slope wedge-shaped joint body is inserted into the triangular pyramid-shaped embedding structure, so that the scion medullary layer and the stock medullary layer are overlapped; wrapping the interface with a composite hydrogel film containing a temperature-sensitive polymer and an antibacterial component; shading treatment is carried out after grafting is completed. The method can significantly improve the survival rate of grafted seedlings.