6 results about "Oospore" patented technology

The invention belongs to the field of gene engineering, and discloses a pythium ultimum PuRWP1 gene and application thereof as a molecular target in prevention and treatment of diseases caused by pythium ultimum. The invention provides a gene PuRWP1 which is from pythium ultimum and is used for controlling a sexual development stage and oospore formation, the gene is composed of 3026 nucleotides, a protein coded by the PuRWP1 gene is composed of 217 amino acids, and knockout of the PuRWP1 gene can lead to remarkable reduction of sporulation ability of a mutant and reduction of spore activity, so that the mutant can be used for preparing a new mutant. The gene can be used as a molecular target to be applied to disease control of oomycetes. The function of the pythium ultimum PuRWP1 gene is verified, and a new direction is provided for further utilizing the gene to regulate and control the physiological functions of oomycetes such as pythium ultimum and preventing and treating oomycetes diseases.

The present application relates to the biological field, and particularly to a soybean Phytophthora psFBP21 protein, a psFBP21 gene and biological materials and application thereof.The amino acid sequence of the soybean Phytophthora psFBP21 protein provided in the present application is shown as SEQ ID NO.2; and the nucleotide sequence of the coding gene is shown as SEQ ID NO.1.Through experiments, it is proved that the soybean Phytophthora psFBP21 protein provided in the present application plays an important role in the growth and development of the soybean Phytophthora itself, and specifically, after the protein is deleted, the mycelium growth of the soybean Phytophthora is slowed down, the number of oospores is reduced, the pathogenicity is reduced, and the like.The above conclusion provides a technical basis for exploring the development and pathogenic molecular mechanism of the soybean Phytophthora, and provides a molecular target for the future research and development of a new fungicide.

The application discloses a kind of egg worm grass special environment climate incubator and process method thereof, belong to the technical field of fungi cultivation, including the following steps: the bird egg inoculated with cordyceps strain is placed in incubator, the temperature in the box is controlled to be the temperature of colonization, humidity is the humidity of colonization, close illumination and send air with the wind speed of colonization, so that cordyceps spore is oriented to germinate inside bird egg;Real-time monitoring of carbon dioxide concentration in the box, dynamically adjust temperature, humidity and ventilation frequency according to the comparison result of monitoring value and multiple preset concentration threshold, so that mycelium proliferates in bird egg;By constructing directional airflow coupling carbon dioxide dynamic feedback control mechanism, the eggshell micro-domain gas environment is fundamentally optimized, low-speed directional air supply forms stable water vapor pressure difference and oxygen concentration gradient on both sides of eggshell stoma, guiding spore germ tube to invade unidirectionally along stoma channel.

The application belongs to the field of genetic engineering, and discloses a final pythium PuRWP1 gene and application of the gene as a molecular target in disease prevention caused by the final pythium. The application provides a gene PuRWP1 from the final pythium for controlling a sexual development stage and oospore formation. The gene is composed of 1184 nucleotides. A protein coded by the PuRWP1 gene is composed of 627 amino acids. Knockout of the PuRWP1 gene can cause a significant decrease in sporulation capacity and a decrease in spore activity of a mutant. The gene can be used as a molecular target in disease prevention of oomycetes. The application verifies the function of the final pythium PuRWP1 gene, and provides a new direction for further regulating physiological functions of the final pythium and other oomycetes and preventing oomycete diseases by using the gene.

The application belongs to the technical field of genetic engineering, and specifically discloses a gene PoRWP2 for improving the production of Pythium oligandrum oospores and an application thereof. The application analyzes and transforms the Pythium oligandrum PoRWP2 gene, which is composed of 837 nucleotides, and the protein coded by the gene contains 278 amino acids, and can regulate the sexual development and oospore formation process of Pythium oligandrum. The application verifies the function of the PoRWP2 gene, provides a new direction for regulating the physiological function of Pythium oligandrum by using the gene and improving the sporulation capacity of the biocontrol bacteria, and provides a theoretical basis and technical support for developing biocontrol agents based on Pythium oligandrum and carrying out biological control of oomycete diseases.

The application discloses a gene PlIRP1 and application thereof in prevention and treatment of litchi blight, and belongs to the technical field of green prevention and control of crop diseases.The application applies a protoplast transformation technology based on polyethylene glycol and a CRISPR / Cas9 knockout strategy, constructs a PlIRP1 knockout vector, and uses PEG-mediated protoplast transformation and a homologous recombination method to knockout the gene PlIRP1 from litchi blight Peronophythora litchii, so as to obtain a knockout mutant.Compared with a wild type, the mutant has obviously weakened pathogenicity and oospore production.The application proves that PlIRP1 is necessary for sexual compatibility and infection pathogenicity of the litchi blight Peronophythora litchii.The research is helpful to further clarify the pathogenic molecular mechanism of the litchi blight Peronophythora litchii, and provides a target gene for development of an effective fungicide.