114 results about "Oospore" patented technology

The invention discloses technology for culturing a unisexual hybrid scallop by utilizing a purple scallop and a bay scallop, which relates to shellfish breeding technology for aquiculture. The technology comprises the steps of: utilizing two congeneric hermaphroditic scallops with same chromosome number; ripening the two scallops respectively to achieve the synchronous maturity; and then synchronously inducing the two scallops to spawn and spermiate; and acquiring sperms and ovum of the two scallops respectively for interspecies cross-insemination to culture the unisexual hybrid scallop. Obtained offsprings have separated sexes, have males and females, and also have hermaphroditic individuals; ovum from female individuals in the hybrid offsprings can be inseminated by any parental sperm and normally grow; and the hermaphroditic individuals in the hybrid offsprings can be used for quickly purifying a strain.

An artificial breeding and culturing method for horseshoe crabs includes: mixing eggs of horseshoe crabs with bodily fluid containing sperms, and allowing the mixture to stand until fertilization; putting the fertilized eggs into filtered seawater to be incubated, and filling oxygen until the eggs are incubated into one-year-old horseshoe crabs; breeding the one-year-old horseshoe crabs at the temperature of from 28 DEG C to 30 DEG C until the crabs slough and develop into two-year-old horseshoe crabs; breeding the two-year-old horseshoe crabs at the temperature of from 20 DEG C to 28 DEG C, and using an air pump to fill oxygen; taking out the two-year-old horseshoe crabs and placing into another container containing bait regularly each day, changing the filtered seawater once a day untilthe crabs are bred into five-year-old crabs; breeding the horseshoe crabs which are five years old or of different ages at the temperature of from 20 DEG C to 28 DEG C, and filling oxygen; taking outthe crabs and breeding in another container containing bait regularly each day, and changing the filtered seawater once a day; and taking out the crabs in breeding and breeding in a container or cement pit with oyster fragments until the crabs are bred into adult horseshoe crabs.

A method for culturing two hybrid varieties of fish includes such steps as providing wuchangfish (or red-scale carp) as female parent and topmouth culter (or red crucian carp) as male parent, injecting oxytocic, taking the mature ova, artificial insemination, incubation, culturing black fry by feeding soybean milk for 10-14 days, and culturing for 4-6 months. Its advantages are unique shape, high growth speed, and high resistance to stress.

The invention discloses a method for inducing gynogenesis of cyprinus carpio L. by using megalobrama amblycephala sperms. The method comprises the following steps of: firstly, selecting a female gynogenesis of cyprinus carpio L. and a male megalobrama amblycephala as parent fishes, secondly, performing artificial induced spawning to obtain megalobrama amblycephala sperms and cyprinus carpio L. spawns; and finally, uniformly mixing ultraviolet-inactivated megalobrama amblycephala seminal sperms and the cyprinus carpio L. spawns, scattering the mixture on a mesh which is flatly paved in water, after 5 minutes, placing activated spawns in cold water of 5 DEG C to perform cold shock treatment for 18 to 25 minutes, and incubating embryos after the cold shock treatment at 21 to 23 DEG C in water to obtain diploid gynogenesis cyprinus carpio L. According to the method, the measures of activating the gynogenesis of the cyprinus carpio L. spawns by using the inactivated megalobrama amblycephala seminal sperms and doubling genomes are used, so that the induced descendants only contain genes of female parents, the homozygosis of genes can be accelerated, and the method has important biological significance in the aspects of color inheritance law study and genetic breeding of the cyprinus carpio L.

The invention relates to an abduction method of a cynoglossus semilaevis diploid fry that is manually induced to be spawned and developed from female nucleus, comprising manually induced spawning of a parent fish, genetic inactivation of sperms, and an induction and identification method of the female nucleus developed diploid fry. The invention firstly establishes a method to obtain large batches of unfertilized ovum by manually induced spawning of the cynoglossus semilaevis, and builds up a method that the cynoglossus semilaevis sperms and lateolabrax japonicus sperms are adopted to induce the developing of the female nucleus of the cynoglossus semilaevis and obtain the female nucleus diploid fry, thus sieving effective manually induced spawning hormones kinds and dosages, and sieving cold shock beginning time and temperature and time that are suitable for the development of the female nucleus of the cynoglossus semilaevis, and the identification method of the female nucleus developed fry of the cynoglossus semilaevis is established. The insemination rate of the ovum of the cynoglossus semilaevis obtained by the method is 60 to 89 percent, the hatch ability is 34 to 79 percent, and the induction rate of the female nucleus developed fry is 0.4 to 6.3 percent. The invention is characterized by advancement, high effect, practicability and reliability, and has important application value and wide popularization prospect on the breeding of fry kinds of the cynoglossus semilaevis, sex control and hologynic breeding.

The invention relates to a method for optimal seedling selection and breeding of novel carassius auratus gibelio series, which comprises the following steps: (1) extracting an egg of a Fangzheng crucian carp; (2) extracting a sperm of a Xingguo red carp; (3) stimulating the female nucleus of the egg of the crucian carps for development to generate an offspring through the sperm of the Xingguo red carp; (3) extracting a sperm of a grass carp and a sperm of a megalobrama amblycephala, and extracting an egg of the offspring generated in the previous step; (5) stimulating the female nucleus of the egg of the offspring generated Step (3) for development with the sperm of the grass carp and the sperm of the megalobrama amblycephala; and (6) breeding the improved seedling. The invention has the following technical effects that: 1) the novel carassius auratus gibelio series with genetic stability can be obtained; 2) the sporozoon resistance of the bred novel carassius auratus gibelio series can be significantly improved; 3) the growth speed of the bred novel carassius auratus gibelio series is high, and the intestinal length / full length is increased, thereby facilitating the improvement of the digestibility; and 4) the thickness of the dorsal muscles and the tightness of the scales of the bred novel carassius auratus gibelio series are improved, the survival rate in the long-distance transport is more than 95 percent, the visceral mass is reduced, the body length is increased, and the intramuscular bones are decreased.

The invention relates to a molecule detection technique of cayenne pepper phytophthora capsici pectin lyase (Pcpel) 1 genes, belonging to the field of molecular biology. A pair of specific primers aredesigned and synthesized by the cayenne (sweet) pepper phytophthora capsici pectin lyase (Pcpel) 1 genes, the specificity of a plurality of tested bacteria species is verified by PCR, and then the sensitivity of the specific primers is verified by carrying out quantitative molecule detection for a soil cayenne pepper phytophthora oospore and a zoospore; and on the basis, the qualitative and quantitative molecule detection and the early warning of cayenne pepper phytophthora capsici contained in cayenne pepper diseased field soil, diseased stems, diseased leaves and diseased fruits are carriedout. The invention can detect and early warn the cayenne pepper phytophthora capsici in different periods and pathopoiesia characters thereof, thereby being convenient to establish a comprehensive prevention and control technology strategy, determining and selecting an optimal prevention and control period to carry out comprehensive prevention and control and high-efficiency management for the cayenne pepper phytophthora capsici, really controlling the infection and the harm of pathogenic bacteria from a source and enhancing the comprehensive prevention and control effect of the cayenne (sweet) pepper phytophthora capsici.

A method capable of promoting stripped shellfish eggs to be mature in vitro is characterized by first stripping off mature shellfish to obtain eggs and sperm, filtering and washing the eggs after stripping, then washing the eggs after being treated by ammonia water at a concentration of 0.005-0.01% for 10-15min and then by 5-hydroxytryptamine at a concentration of 0.001-0.002M for 30-45min, finishing artificial fertilization after combination of the eggs and sperm, which are promoted to be mature in vitro and enabling fertilized eggs to hatch and grow to normal D-type larvae. The shellfish is abalone, bloody clams, sinonovacula constricta, clams, pearl shells or mussels. In the method, the complementary actions of the ammonia water and 5-hydroxytryptamine are adopted, therefore, the respective side effects of the ammonia water and 5-hydroxytryptamine are reduced, the fertilization rate and hatchability are improved and the deformity is less. The method is simple in operation and can be applied to seedling production.

The invention provides a method for breeding tridentiger trigonocephalus by artificial induced spawning and insemination, which relates to the artificial propagation of gobies. The method required tobe provided for artificially breeding the tridentiger trigonocephalus comprises the steps of ripe fish screening, maturity acceleration, insemination, incubation and larval fish culture, wherein the maturity acceleration of ripe fishes uses luteotropin to release hormone analogues, domperidone maleate and chorionic gonadotropin, and is characterized in that the three hormones are used twice for the maturity acceleration; in the maturity acceleration, the water temperature of culture is between 18 and 21 DEG C, the salinity is between 2 and 6, the dissolved oxygen is between 3.5 and 6.5mg / L, and the pH is between 7.5 and 9.0; and after the second time of the maturity acceleration, in 8 to 10 hours, mature ova and mature sperms are selected for artificial insemination which comprises the following steps that: the activated sperms are poured on the ova for sufficient contact so as to obtain fertilized ova; the fertilized ova are placed in a hatchery pond for incubation; and the hatched larval fish is placed in a culture pond for incubation, and chlorella and yolk powder of eggs are placed in water from the third day after the larval fish is hatched to feed food for the larval fish. The method is used for the artificial breeding of the tridentiger trigonocephalus.

The invention relates to a patinopecten yessoensis ternary cross breeding method. Firstly the artificial cultivation accelerated ripening is implemented for the Russian patinopecten yessoensis. Secondly the artificial cultivation accelerated ripening is implemented for the Chinese patinopecten yessoensis. At the same time, the gonad maturation is achieved. A heating mode is utilized to the above patinopecten yessoensis for induced spawning, and the sperms and the eggs are respectively collected. The hybridization is implemented between the Chinese female scallop eggs and the Russian male scallop sperms or between the Chinese male scallop sperms and the Russian female scallop eggs, thus the first filial generation Chinese-Russian or Russian-Chinese two sorts of patinopecten yessoensis colonies which can be cultivated within two years with the conventional suspended longline farming method. The Janpanese patinopecten yessoensis and the Chinese-Russian or the Russian-Chinese patinopecten yessoensis are taken as the parents for the artificial cultivation accelerated ripening. At the same time, the gonad maturation is achieved. A heating mode is utilized to stimulate the induced spawning, and the sperms and the eggs collected are respectively selected for the implementation of hybridization. Thereby the multiple Russian-Chinese-Japanese, Japanese-Chinese-Russian or Janpanese-Russian-Chinese patinopecten yessoensis colonies are obtained, which can be cultivated with the conventional suspended longline farming method. The method is simple, practical with low cost. The patinopecten yessoensis antiviral ability is enhanced. Moreover the survival rate and the livability are high. The offspring heterozygosity is also enhanced through the group fertilization.

The invention discloses a method for carrying out incubation on neolissochilus benasi through artificial fertilization, which belongs to the technical field of fish product breeding. The method comprises the following steps of: injecting DOM (domperidone maleate) and LRH-A2 (lutein releasing hormone A2) into gonadosomatic neolissochilus benasi (1mg of DOM and 1 mu g of LRH per kilogram of neolissochilus benasi are injected in per kilogram of gonadosomatic neolissochilus benasi), wherein the injection volume is halved for male neolissochilus benasi, and the effect time is 15 hours at a water temperature of 22 DEG C; extruding ovums and sperms of the gonadosomatic neolissochilus benasi into a container, stirring the ovums and the sperms for 30 seconds by using chicken feather, then adding clear water at a temperature of 22 DEG C into the container (water preferably just submerges the ovums and the sperms), after stirring the obtained mixture for 20 seconds by using the chicken feather, carrying out washing three times by using clear water, then taking the obtained fertilized ovums for backup incubation; putting the fertilized ovums into an aqueous captan solution (0.15 ppm) to soak for 15 minutes, then putting the fertilized ovums into a water-filled dissecting tray and a shallow-water basin (in which the water level is preferably 3-5 cm higher than the ovums) for incubation, wherein 3000-5000 ovums with an ovum diameter of 2.2-2.8 mm are put in each basin, the position of a water-filled container is 50-80 cm higher than the dissecting tray and the shallow-water basin; sucking water by using a small-sized oxygen hose and conveying the water to the dissecting tray and the shallow-water basin to carry out incubation for 120 hours so as to get fish fries, wherein the temperature of the water is 22-23 DEG C, and the pH value of the water is 7.8-8.5. The method disclosed by the invention has the advantages of low cost, easiness in operation, and high incubation rate.

The present invention relates to an artificial propagation method for veraspei moseri. Its characteristic is to process microscopy examination by artificial taking semen method during October to November, if male fish has been developmental maturation and the quality of semen are good, taking the developmental maturation semen to preserve into liquid nitrogen; each December starting temperature control, to next March early starting rising temperature to 8 DEG C with the rate of 1 DEG every ten days; selecting good gonadal developmental veraspei moseri to process artificial insemination by extruding eggs dried method, mixing the eggs together with extruding semen and freezing semen, injecting sea water to fertilize, the fertilized eggs are put into net cage of hatching tank or hatching pond to manage. The hatching rate is 64 percent to 75 percent generally.

The invention relates to the technology of chromosome operation, in particular to a method for inducing turbot homogenesis gynogenesis fry by using the theory that heredity inactivated sperms are used to perform artificial insemination and a fertilized ovum is restrained from cleavage through hydrostatic pressure. Sexually mature turbot parent fishes are selected, seminal fluid and spawn are collected respectively, insemination is performed on the seminal fluid and the spawn in a volume ratio of 1:100-200, and a control group is formed. The surplus seminal fluid is diluted by a precooled Ringer solution, and sperms are inactivated by ultraviolet rays. Insemination is performed on the inactivated seminal fluid and ova in a volume ratio of 1:10-20. In the 10-20 min before the first fertilized ovum crack appears, the fertilized ovum is placed in a hydrostatic press pressurizing vessel, and processing is performed by the hydrostatic pressure of 55-75 MPa for 6-8 min. Through the method, the 100% turbot homogenesis gynogenesis fry can be inducing obtained efficiently, stably and reliably.

The invention relates to an artificial fertilization and incubation method of sinocyclocheilus grahami, which belongs to the technical field of fishery product culture. The method comprises the steps of dry fertilization and artificial incubation. Intramuscular injection is performed on sexually matured parent sinocyclocheilus grahami with DOM with the use level of 1 mg for per kilogram body weight and luteinizing hormone releasing hormone A2 with the use level of 1 mug for per kilogram body weight; 24 hours later, the mature germ cell and the mature sperm are slightly squeezed out from the abdomens of female sinocyclocheilus grahami and male sinocyclocheilus grahami respectively; the germ cell and the sperm are slightly stirred for 30 seconds in a vessel by chicken feather; clear water is added into the germ cell and the sperm and stirring is performed for 20 seconds; the germ cell and the sperm are cleaned for three times by clear water; the germ cell and the sperm are uniformly spilled onto a palm sheet which is cleaned and sterilized and put into 5 ppm mould solution to be dipped and sterilized for 15 munities, then the germ cell and the sperm are taken out and put into a 20 m or 50 incubating pond to be incubated; an oxygen pump is used for oxygenation to performing incubation under the condition of 18 to 20 DEG C water temperature and 7.5 to 8.0 pH, and after 150 hours, the incubation is accomplished. The method has the advantages that the cost is low, the operation is simple, the method can be put into industrial and intensive production, and the fertilization rate and the incubation rate are high.

The invention relates to a molecule detection technique of cayenne pepper phytophthora capsici polygalacturonase (Pcipg) 5 genes, belonging to the field of molecular biology. A pair of specific primers are designed and synthesized by the cayenne (sweet) pepper phytophthora capsici polygalacturonase (Pcipg) 5 genes, the specificity of a plurality of tested bacteria species is verified by PCR, and then the sensitivity of the specific primers is verified by carrying out quantitative molecule detection for a soil cayenne pepper phytophthora oospore and a zoospore; and on the basis, the qualitativeand quantitative molecule detection and the early warning of cayenne pepper phytophthora capsici contained in cayenne pepper diseased field soil, diseased stems, diseased leaves and diseased fruits are carried out. The invention can detect and early warn the cayenne pepper phytophthora capsici in different periods and pathopoiesia characters thereof, thereby being convenient to establish a comprehensive prevention and control technology strategy, determining and selecting an optimal prevention and control period to carry out comprehensive prevention and control and high-efficiency managementfor the cayenne pepper phytophthora capsici, really controlling the infection and the harm of pathogenic bacteria from a source and enhancing the comprehensive prevention and control effect of the cayenne (sweet) pepper phytophthora capsici.

A crossbreed method for different geographical species parti-colour abalone, which relates to a genetic improved method for parti-colour abalone, especially relates to a crossbreed method using different geographical species parti-colour abalone which comprises, putting the abalone in 16-18Deg C. sea water and elevating temperature to make the parent abalone accelerate. The bait comprises gracilaria 100, Ulva lactuca 30-60, pinnatifida 20-50, spirulina 1-15. Injecting 5-hydroxytryptamine to the head or abdominal feet of the parti-colour abalone, and making the lay eggs and discharge spermium synchronous with a different geographical parti-colour abalone. Making artificial insemination and getting filial generation. and guaranteeing the survival rate during the line haul, making a two species parent parti-colour abalones mature in a same time by using reasonable bait breeding; Using ejecting 5-hydroxytryptamine to make the gamete discharge in a same time and overcome the physiological difference of gamete discharging and sexual maturity because of geographic isolation of different geographical species, and making the gonadal mature and gamete discharge at the same time.

The invention discloses a gynogenesis method of grass carp and application thereof. The method comprises the following steps: taking the diploid sperms of a properly inactivated allotetraploid cruciancarp as a stimulus to stimulate mature eggs of the grass carp, after activating for 2-3 min or fertilizing for 20-22 min, carrying out the water-cooling shock treatment for 10-12 min at 4-6 DEG C, and hatching and controlling the temperature by to obtain the gynogenesis grass carp restraining the discharge of a second polar body or the first cleavage. The method greatly improves the hatching rateand the survival rate of the gynogenesis diploid grass carp, simplifies the preparation process, and can prepare a gynogenesis grass carp colony with good characters and stable heredity.

The present invention relates to method and apparatus for artificial catfish breeding and fertilized egg hatching in circular path. Sexually mature female and male catfish over 3 ages are injected with foreign hormone to induce ovulation and spermiation for artificial fertilization, and the fertilized egg is suspended in flowing water with dissolved oxygen over 6 mg / L inside a circular path for hatching. The hatching apparatus has circular or elliptic pond with circular path. The present invention can realize the large scale production of catfish fly and each of the circular path can hatch fertilized eggs over 10 million in the fertilization rate and hatching rate over 95 %.

A process for culturing the seedlings of Sargassum fusiforme by inducing reproduction and controlling fertilization includes such steps as providing seawater tank, choosing the seed sargassum fusiforme with male and female receptacles, controlling circulating time, illumination intensity and temp for synchronous mature and discharge of sperm and egg, fertilization, collecting fertilized eggs, inoculating the young sporophytes to attachment, and culturing in seawater at 20-25 deg.C for 1-2 months.

The invention discloses a method for inducing gynogenetic diploid of grouper, which comprises the following steps of: diluting semen of the grouper, and radiating the semen by using ultraviolet rays to make the heritability of the semen deactivated; mixing with mature eggs of the grouper; after the eggs are fertilized, transferring to seawater for cold shock treatment to double genomes and generate the gynogenetic diploid; and transferring to seawater, and managing according to common grouper fry to obtain the gynogenetic diploid of the grouper. The offspring generated by induction only contains the gene of the female parent, the homozygosis of good genes can be quickened, and good hereditary features are quickly fixed; and the method can be applied to genetic analysis of the grouper, and has wide application prospect in the aspects of culture and breeding of the grouper.

The invention provides a primer for detecting the soybean phytophthora, and a kit and a method thereof. The primer and the detecting method for detecting the soybean phytophthora have high accuracy, high specificity and high sensitivity, and can detect the various propagules of the soybean phytophthora such as hypha, oospore, zoospore and the like. The whole technique is fast, sensitive and accurate, which has important significance in the prevention of foreign soybean phytophthora, the detection of pathogenic bacteria in domestic affected area, the international trade and the like.

The invention discloses a cultivation method of a gynogenesis megalobrama amblycephala, which comprises the following steps of: firstly selecting and breeding a female megalobrama amblycephala and a male erythroculter ilishaeformis bleeker as parent fishes; then carrying out artificial induced spawning to obtain the sperms of the erythroculter ilishaeformis bleeker and the ripe ovums of the megalobrama amblycephala; mixing the erythroculter ilishaeformis bleeker sperms inactivated by ultraviolet rays with the ripe ovums of the megalobrama amblycephala; 3-4 minutes later, placing the ovums activated at 0-4 DEG C for cold shock treatment for 20-30 minutes; and after incubating embryos treated by cold shock at 23-24 DEG C, obtaining diploid gynogenesis megalobrama amblycephalas through a flow type cell DNA content determination method and a chromosome ploidy detection method of peripheral blood cell culturing. The cultivation method of the gynogenesis megalobrama amblycephala has the advantages of short cultivation time, fast growth speed, high incubation rate, excellent offspring property, and the like, and has great importance on aspects of fish inheritance breeding and biological evolution researches.

The invention discloses a method for breeding ruditapes philippinarum. The method includes steps of selecting individuals with full gonads as parents in ruditapes philippinarum sexual maturity periods; taking gonad samples of the parents; performing microscope examination on the gonad samples in fresh seawater; selecting first certain individuals as female parents and selecting second certain individuals as male parents; placing acquired egg liquid in fresh seawater for 15-45min; activating the egg liquid; placing sperms in the successfully activated egg liquid, performing fertilization in the successfully activated egg liquid, specifically, placing 3-5 sperms around each egg and performing fertilization in a stirring mode; hatching the eggs in a slight inflation manner; cultivating offspring according to a convention process for cultivating the ruditapes philippinarum to acquire large quantities of juvenile mollusks and adults. The first certain individuals have eggs which are microscopically perfectly circular, the eggs are gray black and have complete egg envelopes, the diameters of the eggs are consistent, the diameters of the eggs of northern colonies of the first certain individuals are 70.0+ / -0.5 micrometers, and the diameters of the eggs of southern colonies of the first certain individuals are 60.0+ / -0.5 micrometers. The second certain individuals have the sperms all of which jump microscopically. The method has the advantages of simplicity and convenience in operation, easiness in popularization and the like.

The invention provides a cultivation method for hybridized all-female golden trout fries. An androgen is utilized to induce golden trout parent fish in a mature period so as to obtain golden trout pseudo-milter, steelhead parent fish eggs are fertilized with golden trout pseudo-milter sperms, hatching is performed, and the hybridized all-female golden trout fries are obtained. The eyed rate of the fertilized eggs during a hatching period is over 81%, the fry hatching rate is over 92%, the female rate of the obtained fries is 100% by detection, the rate of golden trout (with golden yellow body color) of the fries is over 90%, the weight gaining rate is over 122% after the fries are cultivated for 30 days, and the survival rate is over 98%. Compared with fries generated by purebred golden trout mating, the cultivation method for the hybridized all-female golden trout fries maintains the golden trout rate to be unchanged, enables the golden trout fries having double advantages of being hybridized and all-female to be obtained, and has good economic benefit and market value.

A method for promoting the sex gland of parent stumpnose bream to be mature and ovulation includes such steps as culturing the parent stumpnose bream in the netted case in nutritive sea region, culturing them in a circulated filter-type reproduction pool when the cocycte is greater than 400 microns in diameter, and injecting synthetic luriberin (LHRH-A2) and chorionic gonadotropin (HCG).

A method for inducing the gynogenesis diploid of large yellow croaker includes such steps as UV irradiating to the diluted sperm of large yellow croaker to deactivate its heredity, mixing it with the ripe ova, starting the gynogenesis of ova, transferring the inseminated ova to seawater, cold shock or static water pressure treating to generate gynogenesis diploid, transferring the inseminated gynogenesis ova in seawater, and ordinary management to obtain the gynogenesis diploid fry.

The invention discloses a natural reeves shad breeding method, which includes the following steps: building of a culture system; parent fish rearing and nutrition fortification; setting of laying nests; laying and fertilized egg collection; hatch of fertilized eggs; fish larva rearing; fry rearing. The natural reeves shad breeding method described by the invention has the advantages that obtained fries are well-developed, and have strong constitutions, moreover, eggs and parent fishes cannot be injured during laying, the fertilization rate of eggs is high, and the survival rate of fish larvae is greatly increased.

The invention discloses a selection method of breeding stichopus japonicus in stichopus japonicus cross breeding. The method comprises the following steps that (1) good breeding stichopus japonicus is collected and screened; (2) the screened breeding stichopus japonicus is placed into a holding pool to be subjected to the maturation acceleration culture through controlling the breeding stichopus japonicus density, the water temperature and the water quality; (3) the breeding stichopus japonicus beginning to enter the breeding period is subjected to miscarriage experiments, the breeding stichopus japonicus is stimulated for spermiation and ovulation, the sex discrimination is carried out, meanwhile, the mass discharge is inhibited, and in addition, male and female individuals are placed into different culture pools to be respectively cultured; (4) the breeding stichopus japonicus is cultured again for two days, the male and female individuals are quantificationally selected according to the requirement on the third day, and are placed into containers for mass discharge, and independent seminal fluid and ova can be obtained; (5) cross breeding or family culture is carried out. The method has the advantages that the fertilization rate of the ova is improved, and the breeding stichopus japonicus waste in the large-scale breeding production is reduced, the fertilization rate and the cross breeding rate are improved, the combination of the sperms and the ova of the stichopus japonicus of the same strain is avoided, and the technical support is provided for the cross breeding of the stichopus japonicus.