Method for breeding ruditapes philippinarum

Abstract

The invention discloses a method for breeding ruditapes philippinarum. The method includes steps of selecting individuals with full gonads as parents in ruditapes philippinarum sexual maturity periods; taking gonad samples of the parents; performing microscope examination on the gonad samples in fresh seawater; selecting first certain individuals as female parents and selecting second certain individuals as male parents; placing acquired egg liquid in fresh seawater for 15-45min; activating the egg liquid; placing sperms in the successfully activated egg liquid, performing fertilization in the successfully activated egg liquid, specifically, placing 3-5 sperms around each egg and performing fertilization in a stirring mode; hatching the eggs in a slight inflation manner; cultivating offspring according to a convention process for cultivating the ruditapes philippinarum to acquire large quantities of juvenile mollusks and adults. The first certain individuals have eggs which are microscopically perfectly circular, the eggs are gray black and have complete egg envelopes, the diameters of the eggs are consistent, the diameters of the eggs of northern colonies of the first certain individuals are 70.0+ / -0.5 micrometers, and the diameters of the eggs of southern colonies of the first certain individuals are 60.0+ / -0.5 micrometers. The second certain individuals have the sperms all of which jump microscopically. The method has the advantages of simplicity and convenience in operation, easiness in popularization and the like.

Description

technical field [0001] The invention relates to the technical field of shellfish genetics and breeding in marine agriculture, in particular to a breeding method for Philippine clams. Background technique [0002] Philippine clams Ruditapes philippinarum Distributed along the coasts of the Pacific Ocean and the Indian Ocean, it has the characteristics of short breeding cycle, low investment and high income (Zhang Guofan & Yan Xiwu, 2010). my country is a big breeding country for Philippine clams, with an annual output of about 3 million tons of Philippine clams, accounting for about 30% of my country's shellfish production, about 20% of the total marine aquaculture output, and accounting for more than 90% of the world's total output (Chinese fishery Association, 2009). Fertilization biology is an important part of developmental biology, which is of great significance both in theory and production practice. It can provide a theoretical basis for breeding fine varieties. [...

AI Technical Summary

Problems solved by technology

However, the fertilization rate of sperm eggs in this method is low. It has been proved by experiments that when the sperm and eggs of shellfish are obtained will directly affect the fertilization rate, but the timing of obtaining eggs and sperm is not disclosed in the above method; at the same time, the method Two kinds of chemicals are used to promote the maturation of the eggs. Although the maturation can be partially achieved, there are still drug residues, which have a certain toxic effect on the fertilization, and the fertilization rate is low (only about 40%). The malformation rate of stage embryos is high (30-50%); and, although a small number of D-shaped larvae can be obtained by the above method, the survival ability of the larvae is poor, and metamorphic spat have not yet been obtained.