5 results about "Sexual maturity" patented technology

This invention discloses a method for large-scale breeding of all-male salmon and trout, belonging to the field of fish sex regulation breeding technology. The method includes: collecting fertilized eggs from different families of the same species of salmon and trout, artificially incubating them to obtain fry, and inducing sex reversal in the fry by using 17β-estradiol when they begin feeding; subsequently, raising the fry to sexual maturity, selecting individuals with a female phenotype, and then using sdY as a sex identification gene, selecting pseudo-females with genotype XY by quantitative real-time PCR; pairing the sexually mature pseudo-females with genotype XY with normal males with genotype XY, and obtaining super-males with genotype YY through artificial insemination; pairing the sexually mature super-males with females with genotype XX, and producing all-male salmon and trout with genotype XY on a large scale through artificial insemination. This invention overcomes the problem that existing technologies cannot achieve large-scale breeding of all-male salmon and trout.

The application belongs to the technical field of animal model construction, and relates to a construction method and application of a zebrafish aging model. The construction method comprises the following steps: 1, sgRNA, Cas9 protein and a homologous recombination knock-in plasmid containing fluorescent protein A and a knock-in gene are transferred into zebrafish embryos, development is cultured to sexual maturity, F0-I is obtained, and a knock-in strain I is obtained through screening; 2, sgRNA, Cas9 protein and a homologous recombination knock-in plasmid containing fluorescent protein B and a knock-in gene are transferred into zebrafish embryos, development is cultured to sexual maturity, F0-II is obtained, and a knock-in strain II is obtained through screening; 3, the knock-in strain I and the knock-in strain II are mated, zebrafish with fluorescent protein A and fluorescent protein B simultaneously expressed in the heart are screened from the offspring, and a wrn gene knockout homozygote, that is, a zebrafish aging model, is obtained. The construction method is more convenient to breed, significantly reduces breeding costs and the workload of screening and breeding, and has good application value.

The application discloses application of an IRS1 gene promoter region SNP site as a pig sexual maturity molecular genetic marker. The present application takes the IRS1 gene promoter region as a research object, and studies the correlation between the IRS1 gene promoter region and sow sexual maturity by using molecular and cell biology methods: it is found that g.128307611C>A and g.128307308C>T are significantly correlated with the age of sexual maturity of Duhei pig populations; then, the influence of different genotypes of the above sites on the activity of the IRS1 gene promoter is studied by transfecting different vectors. Further, the sow ovarian granulosa cells are taken as experimental materials, and experimental techniques such as transcriptome sequencing, total iron colorimetry and malondialdehyde colorimetry are used to study the influence of the IRS1 gene on the transcription level and ferroptosis of the sow ovarian granulosa cells, and it is found that the IRS1 gene influences the different genes of the cell transcriptome, and is mainly enriched in signal pathways such as iron ion homeostasis, and inhibits the ferroptosis of the cells.

This invention provides a method for rapid propagation of superior grass carp families, belonging to the field of aquaculture and fish genetic breeding technology. The method includes: screening for disease-resistant superior families from established grass carp families; isolating and enriching reproductive stem cells from these superior families; transplanting the grass carp family reproductive stem cells into the abdominal cavity or genital ridge region of recipient fish with short sexual maturity cycles; cultivating the recipient fish to sexual maturity, producing sperm, and then fertilizing the sperm with grass carp eggs to obtain F1 generation offspring; performing molecular identification on the offspring to confirm that their genetic material originates from the donor family. This invention can shorten the propagation cycle of superior grass carp families from 4-5 years to approximately 1.5 years, achieving precise and efficient transfer of superior traits, and providing an effective technical means to shorten the grass carp breeding cycle and cultivate superior grass carp varieties.

This invention discloses a method for constructing a zebrafish model of fatty liver based on glucokinase overexpression and its application. The method includes the following steps: using cDNA as a template, a zebrafish GCK gene fragment is obtained by PCR amplification; the vector fragment and the GCK gene fragment are homologously recombined using a homologous recombinase to obtain a recombinant plasmid; the recombinant plasmid is transformed into competent cells, and an endotoxin-free plasmid is extracted as a zebrafish GCK expression vector; a microinjection system is used to inject the plasmid into one-cell-stage fertilized eggs; after the fertilized eggs hatch into juveniles, fluorescent juveniles are selected for further culture until sexual maturity; sexually mature males and females are paired to spawn, and a stably heritable GCK-overexpressing zebrafish strain is selected from the offspring. This invention can obtain a zebrafish strain with stable high GCK expression, which can be used as a zebrafish model of fatty liver based on GCK overexpression.