Low-temperature-resistant trachinotus ovatus fry breeding method

Abstract

The invention provides a low-temperature-resistant trachinotus ovatus fry breeding method which comprises the following steps: (1) parent selection and breeding: taking trachinotus ovatus as a female parent, and carrying out intensive culture before insemination; then low-temperature stress culture is carried out under the condition of 18-20 DEG C, and the survival and normal ingestion parent fishes are used for the subsequent breeding step; (2) cross breeding: screening out sexually mature parents for induced spawning to complete artificial insemination; when the larvae are hatched out of membranes, the larvae are transferred into a breeding pond for fry breeding; (3) feeding rotifer and copepod larvae to the newly hatched larvae; when the larvae are transformed into juvenile fishes, feed containing drosera burmannii is fed, and when the juvenile fishes are transformed into juvenile fishes, feed containing eucommia ulmoides vines is fed for at least 15 days. According to the method, the low-temperature resistance of the trachinotus ovatus fries is effectively improved, and the method is easy to operate, low in cost and easy to apply and popularize.

Description

Technical Field

[0001] The invention relates to the technical field of aquaculture, and in particular to a method for cultivating low-temperature-resistant golden pomfret fry. Background Art

[0002] Golden pomfret (Trachinotus ovatus), scientific name Oval pomfret, is an important aquaculture species, belonging to warm-water fish, with delicious meat, rich nutrition and large market demand. However, golden pomfret seedlings are sensitive to water temperature and have poor low temperature tolerance. The optimum growth temperature is 25℃~35℃, and the low temperature tolerance limit is about 14℃. They grow slowly in a low temperature environment, are prone to disease, and have a low survival rate, which affects the breeding efficiency. In the prior art, the method of cultivating low-temperature resistant golden pomfret fry mainly adopts the following methods: Artificial screening: selecting low-temperature resistant individuals from the parent fish population for breeding, but this method is inefficient and the screening results are unstable. Low temperature acclimation: gradually adapting golden pomfret seedlings to the low temperature environment, but this method has a long cycle and the effect is not obvious. Genetic engineering technology: using genetic engineering technology to transform the golden pomfret gene to make it resistant to low temperatures, but this technology is costly and difficult, and has not yet been widely used. Therefore, there is an urgent need to develop an efficient, safe and economical method for cultivating low-temperature-resistant golden pomfret fry. Summary of the invention

[0003] In view of the above problems existing in the prior art, the purpose of the present invention is to provide a method for cultivating low-temperature resistant golden pomfret fry to solve the problems of low efficiency, unclear effect, high cost and high risk in cultivating low-temperature resistant golden pomfret fry.

[0004] In order to solve the above technical problems, the technical solution adopted by the present invention is as follows:

[0005] A method for cultivating low-temperature resistant golden pomfret fry comprises the following steps:

[0006] (1) Parent selection and breeding:

[0007] Using the oval pomfret as the female parent, before fertilization, intensive culture is carried out; then short-term low-temperature stress culture is carried out at 18-20°C, and the broodstock that survives and eats normally are used for subsequent breeding steps;

[0008] (2) Reproduction:

[0009] The sexually mature parents are induced to give birth and artificial insemination is completed; when the fry are hatched, they are transferred to the breeding pond for fry breeding;

[0010] (3) Newly hatched fry are fed with rotifers and copepod larvae; when the fry metamorphose into juveniles, they are fed with feed containing Myrica chinensis; when the juveniles metamorphose into juveniles, they are fed with feed containing Eucommia ulmoides for at least 15 days.

[0011] Preferably, the short-term low temperature stress culture time is 24 hours.

[0012] Preferably, in the feed containing brocade, the content of brocade is 40% to 43.5%.

[0013] Preferably, in the feed containing Eucommia ulmoides, the content of Eucommia ulmoides is 24.1% to 25%.

[0014] Preferably, the feed containing Jindiluo contains the following ingredients, in parts by weight: 400-430 parts of fish meal, 70-80 parts of corn protein, 35-40 parts of fish oil, 2-5 parts of brewer's yeast powder, 20-25 parts of glutamine, 10-12 parts of yeast selenium, 2-3 parts of sodium acetate, 6-7 parts of Yunnan red rose, 8-10 parts of γ-aminobutyric acid, and 400-435 parts of Jindiluo.

[0015] Preferably, the feed containing Eucommia ulmoides is: measured by weight, containing the following ingredients: 500-520 parts of fish meal, 50-70 parts of fish oil, 30-40 parts of bran, 60-70 parts of wheat gluten powder, 6-10 parts of brewer's yeast powder, 2-5 parts of shrimp shell powder, 3-4 parts of squid paste, 23-30 parts of wheat germ powder, 25-30 parts of peanut meal, 10-13 parts of palm oil, 3-5 parts of calcium dihydrogen phosphate, and 241-250 parts of Eucommia ulmoides.

[0016] Preferably, during the intensive culture, the feed used is:

[0017] Calculated by mass: 30 parts of fish meal, 1 part of squid, 2 parts of sardines, 2 parts of oysters, 2 parts of lugworms, 5 parts of fish oil, 1 part of vegetable oil, 0.5 part of calcium hydrogen phosphate, 0.5 part of multivitamins, and 0.5 part of multiminerals.

[0018] Preferably, the feed containing Jindiluo is composed of the following components in parts by weight: 430 parts of fish meal, 80 parts of corn protein, 40 parts of fish oil, 2 parts of brewer's yeast powder, 20 parts of glutamine, 10 parts of yeast selenium, 2 parts of sodium acetate, 6 parts of Yunnan red rose, 10 parts of γ-aminobutyric acid, and 400 parts of Jindiluo.

[0019] Preferably, the feed containing Eucommia ulmoides is composed of the following components, by weight: 520 parts of fish meal, 50 parts of fish oil, 30 parts of bran, 70 parts of wheat gluten powder, 6 parts of brewer's yeast powder, 2 parts of shrimp shell powder, 3 parts of squid paste, 23 parts of wheat germ powder, 30 parts of peanut meal, 13 parts of palm oil, 3 parts of monocalcium phosphate, and 250 parts of Eucommia ulmoides.

[0020] Preferably, the parents are Trachinotus ovatus as the female parent and Trachinotus blochii as the male parent.

[0021] The present invention aims at the shortcomings of the existing golden pomfret fry breeding method and makes many improvements. First, in terms of parent selection and breeding, 3-4 years old, 3.5-4 kg, strong and healthy oval pomfret is carefully selected as broodstock, and low temperature stress treatment is carried out to screen out low temperature resistant broodstock. At each stage of fry breeding, bait is fed reasonably, and Jindiluo and Eucommia ulmoides are added to the feed to improve the nutritional metabolism of the fish body, so that the fry can better resist the influence of low temperature, thereby improving the low temperature survival rate of the fry.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The method of the invention is simple to operate, low in cost, and easy to promote and apply. Experimental verification shows that the low temperature resistance and survival rate of the golden pomfret fry cultivated by the method of the invention are significantly improved.

[0024] The invention effectively solves the problem of adaptability of golden pomfret fry when it is cultured in low-temperature areas or transferred from tropical areas to low-temperature areas, is beneficial to the breeding of golden pomfret in low-temperature areas, reduces the temperature control cost of breeding factories, reduces breeding losses, and improves economic benefits.

[0025] Compared with hybrid breeding, transgenic breeding and low temperature stress induction, the method of the present invention is simple to operate and has a short processing cycle, and is more suitable for actual production. DETAILED DESCRIPTION

[0026] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0027] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0028] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0029] The vitamin complex consists of VB1, VB2, VB6, VB12 and VC in equal mass ratios.

[0030] The composite mineral is composed of the following components in parts by weight: 54 parts of calcium carbonate, 12 parts of calcium hydrogen phosphate, 17 parts of table salt, 3 parts of copper methionine, 5 parts of ferrous glycinate, 2 parts of manganese methionine, 6 parts of zinc lysine, and 1 part of calcium iodate.

[0031] The Eucommia ulmoides described in the present invention refers to the root of Parabarium huaitingChun et Tsiang, a plant of the Apocynaceae family. The root is dried, crushed, and then added to the feed.

[0032] The Drosera burmanni Vahl described in the present invention has a Latin name of Drosera burmanni Vahl, which is the whole herb of the herbaceous plant of the Drosera family and the genus Drosera. The whole herb is dried and crushed and then added to the feed.

[0033] The cassia seeds of the present invention are dried mature seeds of Cassia tora L., a leguminous plant, which are crushed and added to feed.

[0034] Example 1 A method for cultivating low-temperature resistant golden pomfret fry

[0035] (1) Parent selection and breeding:

[0036] Select 3-4 years old, 3.5-4 kg, strong and healthy oval pomfret as the female parent and Brandt's pomfret as the male parent;

[0037] Intensive culture was carried out for the first 6 months of cross fertilization of broodstock. Feed was given twice a day, and the daily feeding amount was 5% of the fish body weight. The broodstock feed was: by weight, 30 parts of fish meal, 1 part of squid, 2 parts of sardines, 2 parts of oysters, 2 parts of sandworms, 5 parts of fish oil, 1.5 parts of vegetable oil, 0.5 parts of calcium hydrogen phosphate, 0.5 parts of multivitamins, and 0.5 parts of multiminerals.

[0038] The fish were subjected to stress treatment at 18-20℃ for 24 hours, and the ovate pomfret and the brandt pomfret that survived and ate normally were used as cold-resistant broodstock;

[0039] (2) Hybrid breeding:

[0040] The sexually mature parents are induced to give birth and artificial insemination is completed; when the fry hatch out of the membrane, they are transferred to the breeding pond for fry cultivation;

[0041] (3) Newly hatched fry are fed with rotifers and copepod larvae; when the fry metamorphose into juveniles, they are fed with feed containing nematodes; when the juveniles metamorphose into juveniles, they are fed with feed containing Eucommia ulmoides for at least 15 days. The daily feed intake is 5% to 7% of the fish body weight.

[0042] The feed containing brocade is composed of the following components by weight: 430 parts of fish meal, 80 parts of corn protein, 40 parts of fish oil, 2 parts of brewer's yeast powder, 20 parts of glutamine, 10 parts of yeast selenium, 2 parts of sodium acetate, 6 parts of Yunnan red rose, 10 parts of gamma-aminobutyric acid, and 400 parts of brocade.

[0043] The feed containing Eucommia ulmoides is composed of the following components, by weight: 520 parts of fish meal, 50 parts of fish oil, 30 parts of bran, 70 parts of wheat gluten powder, 6 parts of brewer's yeast powder, 2 parts of shrimp shell powder, 3 parts of squid paste, 23 parts of wheat germ powder, 30 parts of peanut meal, 13 parts of palm oil, 3 parts of calcium dihydrogen phosphate, and 250 parts of Eucommia ulmoides.

[0044] Example 2 A method for cultivating low-temperature resistant golden pomfret fry

[0045] (1) Parent selection and breeding:

[0046] Select 3-4 years old, 3.5-4 kg, strong and healthy oval pomfret as the female parent and Brandt's pomfret as the male parent;

[0047] Intensive culture was carried out for the first 6 months of cross fertilization of broodstock. Feed was given twice a day, and the daily feeding amount was 5% of the fish body weight. The broodstock feed was: by weight, 30 parts of fish meal, 1 part of squid, 2 parts of sardines, 2 parts of oysters, 2 parts of sandworms, 5 parts of fish oil, 1.5 parts of vegetable oil, 0.5 parts of calcium hydrogen phosphate, 0.5 parts of multivitamins, and 0.5 parts of multiminerals.

[0048] The fish were subjected to stress treatment at 18-20℃ for 24 hours, and the ovate pomfret and the brandt pomfret that survived and ate normally were used as cold-resistant broodstock;

[0049] (2) Hybrid breeding:

[0050] The sexually mature parents are induced to give birth and artificial insemination is completed; when the fry hatch out of the membrane, they are transferred to the breeding pond for fry cultivation;

[0051] (3) Newly hatched fry are fed with rotifers and copepod larvae; when the fry metamorphose into juveniles, they are fed with feed containing nematodes; when the juveniles metamorphose into juveniles, they are fed with feed containing Eucommia ulmoides for at least 15 days. The daily feed intake is 5% to 7% of the fish body weight.

[0052] The feed containing brocade is composed of the following components by weight: 400 parts of fish meal, 70 parts of corn protein, 35 parts of fish oil, 5 parts of brewer's yeast powder, 25 parts of glutamine, 12 parts of yeast selenium, 3 parts of sodium acetate, 7 parts of Yunnan red rose, 8 parts of gamma-aminobutyric acid, and 435 parts of brocade.

[0053] The feed containing Eucommia ulmoides is composed of the following components, by weight: 500 parts of fish meal, 70 parts of fish oil, 40 parts of bran, 60 parts of wheat gluten powder, 10 parts of brewer's yeast powder, 5 parts of shrimp shell powder, 4 parts of squid paste, 30 parts of wheat germ powder, 25 parts of peanut meal, 10 parts of palm oil, 5 parts of calcium dihydrogen phosphate, and 241 parts of Eucommia ulmoides.

[0054] Comparative Example 1 A method for cultivating low-temperature resistant golden pomfret fry

[0055] (1) Parent selection and breeding:

[0056] Select 3-4 years old, 3.5-4 kg, strong and healthy oval pomfret as the female and male parents;

[0057] Intensive culture was carried out for the first 6 months of cross fertilization of broodstock. Feed was given twice a day, and the daily feeding amount was 5% of the fish body weight. The broodstock feed was: by weight, 30 parts of fish meal, 1 part of squid, 2 parts of sardines, 2 parts of oysters, 2 parts of sandworms, 5 parts of fish oil, 1.5 parts of vegetable oil, 0.5 parts of calcium hydrogen phosphate, 0.5 parts of multivitamins, and 0.5 parts of multiminerals.

[0058] The golden pomfret was subjected to stress treatment at a temperature of 18-20°C for 24 hours, and the survived and normally fed golden pomfret was used as the cold-resistant broodstock;

[0059] (2) Hybrid breeding:

[0060] Select sexually mature parents for induced spawning and artificial insemination; when the fry are hatched, they are transferred to the breeding pond for fry cultivation;

[0061] (3) Newly hatched fry are fed with rotifers and copepod larvae; when the fry metamorphose into juveniles, they are fed with feed containing nematodes; when the juveniles metamorphose into juveniles, they are fed with feed containing Eucommia ulmoides for at least 15 days. The daily feed intake is 5% to 7% of the fish body weight.

[0062] The feed containing brocade is composed of the following components by weight: 430 parts of fish meal, 80 parts of corn protein, 40 parts of fish oil, 2 parts of brewer's yeast powder, 20 parts of glutamine, 10 parts of yeast selenium, 2 parts of sodium acetate, 6 parts of Yunnan red rose, 10 parts of gamma-aminobutyric acid, and 400 parts of brocade.

[0063] The feed containing Eucommia ulmoides is composed of the following components, by weight: 520 parts of fish meal, 50 parts of fish oil, 30 parts of bran, 70 parts of wheat gluten powder, 6 parts of brewer's yeast powder, 2 parts of shrimp shell powder, 3 parts of squid paste, 23 parts of wheat germ powder, 30 parts of peanut meal, 13 parts of palm oil, 3 parts of calcium dihydrogen phosphate, and 250 parts of Eucommia ulmoides.

[0064] Comparative Example 2 A method for cultivating low-temperature resistant golden pomfret fry

[0065] (1) Parent selection and breeding:

[0066] Select 3-4 years old, 3.5-4 kg, strong and healthy oval pomfret as the female parent and Brandt's pomfret as the male parent;

[0067] Intensive culture was carried out for the first 6 months of cross fertilization of broodstock. Feed was given twice a day, and the daily feeding amount was 5% of the fish body weight. The broodstock feed was: by weight, 30 parts of fish meal, 1 part of squid, 2 parts of sardines, 2 parts of oysters, 2 parts of sandworms, 5 parts of fish oil, 1.5 parts of vegetable oil, 0.5 parts of calcium hydrogen phosphate, 0.5 parts of multivitamins, and 0.5 parts of multiminerals.

[0068] (2) Hybrid breeding:

[0069] The sexually mature parents are induced to give birth and artificial insemination is completed; when the fry hatch out of the membrane, they are transferred to the breeding pond for fry cultivation;

[0070] (3) Newly hatched fry are fed with rotifers and copepod larvae; when the fry metamorphose into juveniles, they are fed with feed containing nematodes; when the juveniles metamorphose into juveniles, they are fed with feed containing Eucommia ulmoides for at least 15 days. The daily feed intake is 5% to 7% of the fish body weight.

[0071] The feed containing brocade is composed of the following components by weight: 430 parts of fish meal, 80 parts of corn protein, 40 parts of fish oil, 2 parts of brewer's yeast powder, 20 parts of glutamine, 10 parts of yeast selenium, 2 parts of sodium acetate, 6 parts of Yunnan red rose, 10 parts of gamma-aminobutyric acid, and 400 parts of brocade.

[0072] The feed containing Eucommia ulmoides is composed of the following components, by weight: 520 parts of fish meal, 50 parts of fish oil, 30 parts of bran, 70 parts of wheat gluten powder, 6 parts of brewer's yeast powder, 2 parts of shrimp shell powder, 3 parts of squid paste, 23 parts of wheat germ powder, 30 parts of peanut meal, 13 parts of palm oil, 3 parts of calcium dihydrogen phosphate, and 250 parts of Eucommia ulmoides.

[0073] Comparative Example 3 A method for cultivating low-temperature resistant golden pomfret fry

[0074] (1) Parent selection and breeding:

[0075] Select 3-4 years old, 3.5-4 kg, strong and healthy oval pomfret as the female parent and Brandt's pomfret as the male parent;

[0076] Intensive culture was carried out for the first 6 months of cross fertilization of broodstock. Feed was given twice a day, and the daily feeding amount was 5% of the fish body weight. The broodstock feed was: by weight, 30 parts of fish meal, 1 part of squid, 2 parts of sardines, 2 parts of oysters, 2 parts of sandworms, 5 parts of fish oil, 1.5 parts of vegetable oil, 0.5 parts of calcium hydrogen phosphate, 0.5 parts of multivitamins, and 0.5 parts of multiminerals.

[0077] The fish were subjected to stress treatment at 18-20℃ for 24 hours, and the ovate pomfret and the brandt pomfret that survived and ate normally were used as cold-resistant broodstock;

[0078] (2) Hybrid breeding:

[0079] The sexually mature parents are induced to give birth and artificial insemination is completed; when the fry are hatched, they are transferred to the breeding pond for fry breeding;

[0080] (3) Newly hatched fry are fed with rotifers and copepod larvae; when the fry metamorphose into juveniles, they are fed with feed containing Cassia seed; when the juveniles metamorphose into juveniles, they are fed with feed containing Eucommia ulmoides for at least 15 days. The daily feed intake is 5% to 7% of the fish body weight.

[0081] The feed containing cassia seeds is composed of the following components in parts by weight: 430 parts of fish meal, 80 parts of corn protein, 40 parts of fish oil, 2 parts of brewer's yeast powder, 20 parts of glutamine, 10 parts of yeast selenium, 2 parts of sodium acetate, 6 parts of Yunnan red rose, 10 parts of gamma-aminobutyric acid, and 400 parts of cassia seeds.

[0082] The feed containing cassia seeds is composed of the following components, by weight: 520 parts of fish meal, 50 parts of fish oil, 30 parts of bran, 70 parts of wheat gluten powder, 6 parts of brewer's yeast powder, 2 parts of shrimp shell powder, 3 parts of squid paste, 23 parts of wheat germ powder, 30 parts of peanut meal, 13 parts of palm oil, 3 parts of calcium dihydrogen phosphate, and 250 parts of cassia seeds.

[0083] Comparative Example 4: A method for cultivating low-temperature resistant golden pomfret fry

[0084] (1) Parent selection and breeding:

[0085] Select 3-4 years old, 3.5-4 kg, strong and healthy oval pomfret as the female parent and Brandt's pomfret as the male parent;

[0086] Intensive culture was carried out for the first 6 months of cross fertilization of broodstock. Feed was given twice a day, and the daily feeding amount was 5% of the fish body weight. The broodstock feed was: by weight, 30 parts of fish meal, 1 part of squid, 2 parts of sardines, 2 parts of oysters, 2 parts of sandworms, 5 parts of fish oil, 1.5 parts of vegetable oil, 0.5 parts of calcium hydrogen phosphate, 0.5 parts of multivitamins, and 0.5 parts of multiminerals.

[0087] The fish were subjected to stress treatment at 18-20℃ for 24 hours, and the ovate pomfret and the brandt pomfret that survived and ate normally were used as cold-resistant broodstock;

[0088] (2) Hybrid breeding:

[0089] The sexually mature parents are induced to give birth and artificial insemination is completed; when the fry are hatched, they are transferred to the breeding pond for fry breeding;

[0090] (3) Newly hatched fry are fed with rotifers and copepod larvae; when the fry metamorphose into juveniles, they are fed with feed A; when the juveniles metamorphose into juveniles, they are fed with feed containing Eucommia ulmoides for at least 15 days. The daily feed amount is 5% to 7% of the fish body weight.

[0091] The feed A is composed of the following components by weight: 430 parts of fish meal, 80 parts of corn protein, 40 parts of fish oil, 2 parts of brewer's yeast powder, 20 parts of glutamine, 10 parts of yeast selenium, 2 parts of sodium acetate, 6 parts of Yunnan red rose, and 10 parts of gamma-aminobutyric acid.

[0092] The feed containing Eucommia ulmoides is composed of the following components, by weight: 520 parts of fish meal, 50 parts of fish oil, 30 parts of bran, 70 parts of wheat gluten powder, 6 parts of brewer's yeast powder, 2 parts of shrimp shell powder, 3 parts of squid paste, 23 parts of wheat germ powder, 30 parts of peanut meal, 13 parts of palm oil, 3 parts of calcium dihydrogen phosphate, and 250 parts of Eucommia ulmoides.

[0093] Comparative Example 5: A method for cultivating low-temperature resistant golden pomfret fry

[0094] (1) Parent selection and breeding:

[0095] Select 3-4 years old, 3.5-4 kg, strong and healthy oval pomfret as the female parent and Brandt's pomfret as the male parent;

[0096] Intensive culture was carried out for the first 6 months of cross fertilization of broodstock. Feed was given twice a day, and the daily feeding amount was 5% of the fish body weight. The broodstock feed was: by weight, 30 parts of fish meal, 1 part of squid, 2 parts of sardines, 2 parts of oysters, 2 parts of sandworms, 5 parts of fish oil, 1.5 parts of vegetable oil, 0.5 parts of calcium hydrogen phosphate, 0.5 parts of multivitamins, and 0.5 parts of multiminerals.

[0097] The fish were subjected to stress treatment at 18-20℃ for 24 hours, and the ovate pomfret and the brandt pomfret that survived and ate normally were used as cold-resistant broodstock;

[0098] (2) Hybrid breeding:

[0099] The sexually mature parents are induced to give birth and artificial insemination is completed; when the fry are hatched, they are transferred to the breeding pond for fry breeding;

[0100] (3) Newly hatched fry are fed with rotifers and copepod larvae; when the fry metamorphose into juveniles, they are fed with feed containing nematodes; when the juveniles metamorphose into juveniles, they are fed with feed A for at least 15 days. The daily feed amount is 5% to 7% of the fish body weight.

[0101] The feed containing brocade is composed of the following components by weight: 430 parts of fish meal, 80 parts of corn protein, 40 parts of fish oil, 2 parts of brewer's yeast powder, 20 parts of glutamine, 10 parts of yeast selenium, 2 parts of sodium acetate, 6 parts of Yunnan red rose, 10 parts of gamma-aminobutyric acid, and 400 parts of brocade.

[0102] The feed A is composed of the following components by weight: 520 parts of fish meal, 50 parts of fish oil, 30 parts of bran, 70 parts of wheat gluten powder, 6 parts of brewer's yeast powder, 2 parts of shrimp shell powder, 3 parts of squid paste, 23 parts of wheat germ powder, 30 parts of peanut meal, 13 parts of palm oil, and 3 parts of monocalcium phosphate.

[0103] Comparative Example 6: A method for cultivating low-temperature resistant golden pomfret fry

[0104] (1) Parent selection and breeding:

[0105] Select 3-4 years old, 3.5-4 kg, strong and healthy oval pomfret as the female parent and Brandt's pomfret as the male parent;

[0106] Intensive culture was carried out for the first 6 months of cross fertilization of broodstock. Feed was given twice a day, and the daily feeding amount was 5% of the fish body weight. The broodstock feed was: by weight, 30 parts of fish meal, 1 part of squid, 2 parts of sardines, 2 parts of oysters, 2 parts of sandworms, 5 parts of fish oil, 1.5 parts of vegetable oil, 0.5 parts of calcium hydrogen phosphate, 0.5 parts of multivitamins, and 0.5 parts of multiminerals.

[0107] The golden pomfret was subjected to stress treatment at a temperature of 18-20°C for 24 hours, and the survived and normally fed golden pomfret was used as the cold-resistant broodstock;

[0108] (2) Hybrid breeding:

[0109] The sexually mature parents are induced to give birth and artificial insemination is completed; when the fry are hatched, they are transferred to the breeding pond for fry breeding;

[0110] (3) Newly hatched fry are fed with rotifers and copepod larvae; when the fry metamorphose into juveniles, they are fed with feed containing Eucommia ulmoides; when the juveniles metamorphose into juveniles, they are fed with feed containing Myrica chinensis for at least 15 days. The daily feed intake is 5% to 7% of the fish body weight.

[0111] The feed containing Eucommia ulmoides is composed of the following components, by weight: 430 parts of fish meal, 80 parts of corn protein, 40 parts of fish oil, 2 parts of brewer's yeast powder, 20 parts of glutamine, 10 parts of yeast selenium, 2 parts of sodium acetate, 6 parts of Yunnan red rose, 10 parts of gamma-aminobutyric acid, and 400 parts of Eucommia ulmoides.

[0112] The feed containing brocade is composed of the following components by weight: 520 parts of fish meal, 50 parts of fish oil, 30 parts of bran, 70 parts of wheat gluten powder, 6 parts of brewer's yeast powder, 2 parts of shrimp shell powder, 3 parts of squid paste, 23 parts of wheat germ powder, 30 parts of peanut meal, 13 parts of palm oil, 3 parts of calcium dihydrogen phosphate, and 250 parts of brocade.

[0113] Seedling cultivation effect detection:

[0114] The activity of aspartate aminotransferase in serum can reflect the damage of fish. When the liver or myocardium is damaged, the activity of aspartate aminotransferase will increase significantly. The serum of golden pomfret was collected and AST was measured using an aspartate aminotransferase (AST / GOT) kit (microplate method).

[0115] 200 fry (i.e., fry that have metamorphosed from young fish to fingerlings and cultured with corresponding feeds for 15 days) were randomly selected from each embodiment and comparative example, and the temperature was gradually lowered from the original culture temperature of 28-30°C to 15-17°C at a rate of 2°C / h, while other culture conditions remained unchanged, and the survival rate after culture at 15-17°C for 72 hours was measured.

[0116] Each group of experiments was repeated 3 times, and the experimental results were expressed as the average value.

[0117] Table 1

[0118]

[0119] In comparative example 1, the oval pomfret was used as the male and female parents, the parents of comparative example 2 were not subjected to low temperature stress treatment, comparative example 3 replaced scutellaria baicalensis and eucommia ulmoides with cassia seed, comparative example 4 did not add scutellaria baicalensis, comparative example 5 did not add eucommia ulmoides, and comparative example 6 replaced scutellaria baicalensis and eucommia ulmoides.

[0120] The results show that the aspartate aminotransferase activity of Examples 1 and 2 is the lowest, indicating that the fish body is less damaged and there is no obvious nutritional metabolic disorder. The golden pomfret fry can survive in large numbers under certain low temperature conditions, and the survival rate reaches more than 93%. The aspartate aminotransferase activity of Comparative Examples 1 to 6 is higher, and the survival rate at low temperature is low. By comparing the results, the following conclusions can be preliminarily drawn: the fry bred by the parents using the corresponding fortified feed and further subjected to low temperature stress can better adapt to low temperature conditions and have a higher survival rate. The addition of Jindi Luo and Eucommia ulmoides can improve the metabolism of the fish body and make the fry more resistant to the influence of low temperature. The seedling breeding method provided by the present invention is more suitable for the offspring of the hybridization of the oval pomfret and the Brandt's pomfret, and can improve the survival rate and low temperature resistance of the fry.

[0121] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for cultivating low-temperature resistant golden pomfret fry, characterized in that: The following steps are involved: (1) Parent selection and breeding: Using the oval pomfret as the female parent, before fertilization, intensive culture is carried out; then short-term low-temperature stress culture is carried out at 18-20°C, and the broodstock that survives and eats normally are used for subsequent breeding steps; (2) Reproduction: The sexually mature parents are induced to give birth and artificial insemination is completed; when the fry are hatched, they are transferred to the breeding pond for fry breeding; (3) Newly hatched fry are fed with rotifers and copepod larvae; when the fry metamorphose into juveniles, they are fed with feed containing Myrica chinensis; when the juveniles metamorphose into juveniles, they are fed with feed containing Eucommia ulmoides for at least 15 days.

2. A method for cultivating low-temperature-resistant golden pomfret fry according to claim 1, characterized in that: The short-term low temperature stress culture time is 24 hours.

3. A method for cultivating low-temperature-resistant golden pomfret fry according to claim 1, characterized in that: In the feed containing brocade, the content of brocade is 40% to 43.5%.

4. A method for cultivating low-temperature-resistant golden pomfret fry according to claim 1, characterized in that: In the feed containing Eucommia ulmoides, the content of Eucommia ulmoides is 24.1%-25%.

5. A method for cultivating low-temperature-resistant golden pomfret fry according to claim 1, characterized in that: The feed containing brocade contains the following ingredients in parts by weight: 400-430 parts of fish meal, 70-80 parts of corn protein, 35-40 parts of fish oil, 2-5 parts of brewer's yeast powder, 20-25 parts of glutamine, 10-12 parts of yeast selenium, 2-3 parts of sodium acetate, 6-7 parts of Yunnan red rose, 8-10 parts of gamma-aminobutyric acid, and 400-435 parts of brocade.

6. A method for cultivating low-temperature-resistant golden pomfret fry according to claim 1, characterized in that: The feed containing Eucommia ulmoides is: measured by weight, containing the following ingredients: 500-520 parts of fish meal, 50-70 parts of fish oil, 30-40 parts of bran, 60-70 parts of wheat gluten powder, 6-10 parts of brewer's yeast powder, 2-5 parts of shrimp shell powder, 3-4 parts of squid paste, 23-30 parts of wheat germ powder, 25-30 parts of peanut meal, 10-13 parts of palm oil, 3-5 parts of calcium dihydrogen phosphate, and 241-250 parts of Eucommia ulmoides.

7. A method for cultivating low-temperature-resistant golden pomfret fry according to claim 1, characterized in that: During the intensive culture, the feed used is: Calculated by mass: 30 parts of fish meal, 1 part of squid, 2 parts of sardines, 2 parts of oysters, 2 parts of lugworms, 5 parts of fish oil, 1.5 parts of vegetable oil, 0.5 parts of calcium hydrogen phosphate, 0.5 parts of multivitamins, and 0.5 parts of multiminerals.

8. A method for cultivating low-temperature-resistant golden pomfret fry according to claim 1, characterized in that: The feed containing brocade is composed of the following components by weight: 430 parts of fish meal, 80 parts of corn protein, 40 parts of fish oil, 2 parts of brewer's yeast powder, 20 parts of glutamine, 10 parts of yeast selenium, 2 parts of sodium acetate, 6 parts of Yunnan red rose, 10 parts of gamma-aminobutyric acid, and 400 parts of brocade.

9. A method for cultivating low-temperature-resistant golden pomfret fry according to claim 1, characterized in that: The feed containing Eucommia ulmoides is composed of the following components, by weight: 520 parts of fish meal, 50 parts of fish oil, 30 parts of bran, 70 parts of wheat gluten powder, 6 parts of brewer's yeast powder, 2 parts of shrimp shell powder, 3 parts of squid paste, 23 parts of wheat germ powder, 30 parts of peanut meal, 13 parts of palm oil, 3 parts of calcium dihydrogen phosphate, and 250 parts of Eucommia ulmoides.

10. A method for cultivating low-temperature resistant golden pomfret fry according to claim 1, characterized in that: The parents are the oval pomfret female parent and the Brandt's pomfret male parent.

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